Base de dados : MEDLINE
Pesquisa : B01.050.500.802.080 [Categoria DeCS]
Referências encontradas : 33 [refinar]
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  1 / 33 MEDLINE  
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[PMID]:27419263
[Au] Autor:El-Demerdash A; Moriou C; Martin MT; Rodrigues-Stien Ade S; Petek S; Demoy-Schneider M; Hall K; Hooper JN; Debitus C; Al-Mourabit A
[Ad] Endereço:Institut de Chimie des Substances Naturelles, CNRS UPR 2301, Univ. Paris-Sud, Université Paris-Saclay , 1, Avenue de la Terrasse, 91198 Gif-sur-Yvette, France.
[Ti] Título:Cytotoxic Guanidine Alkaloids from a French Polynesian Monanchora n. sp. Sponge.
[So] Source:J Nat Prod;79(8):1929-37, 2016 Aug 26.
[Is] ISSN:1520-6025
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Four bicyclic and three pentacyclic guanidine alkaloids (1-7) were isolated from a French Polynesian Monanchora n. sp. sponge, along with the known alkaloids monalidine A (8), enantiomers 9-11 of known natural product crambescins, and the known crambescidins 12-15. Structures were assigned by spectroscopic data interpretation. The relative and absolute configurations of the alkaloids were established by analysis of (1)H NMR and NOESY spectra and by circular dichroism analysis. The new norcrambescidic acid (7) corresponds to interesting biosynthetic variation within the pentacyclic core. All compounds exhibited antiproliferative and cytotoxic efficacy against KB, HCT116, HL60, MRC5, and B16F10 cancer cells, with IC50 values ranging from 4 nM to 10 µM.
[Mh] Termos MeSH primário: Alcaloides/isolamento & purificação
Alcaloides/farmacologia
Antineoplásicos/isolamento & purificação
Antineoplásicos/farmacologia
Axinella/química
Guanidinas/isolamento & purificação
Guanidinas/farmacologia
[Mh] Termos MeSH secundário: Alcaloides/química
Animais
Antineoplásicos/química
Ensaios de Seleção de Medicamentos Antitumorais
Guanidinas/química
Células HCT116
Células HL-60
Seres Humanos
Concentração Inibidora 50
Células KB
Biologia Marinha
Ressonância Magnética Nuclear Biomolecular
Polinésia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkaloids); 0 (Antineoplastic Agents); 0 (Guanidines)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170502
[Lr] Data última revisão:
170502
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160716
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jnatprod.6b00168


  2 / 33 MEDLINE  
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[PMID]:24493364
[Au] Autor:Margarucci L; Monti MC; Esposito R; Tosco A; Hamel E; Riccio R; Casapullo A
[Ad] Endereço:Dipartimento di Farmacia, Università degli Studi di Salerno, Via Giovanni Paolo II 132, 84084 Fisciano, Italy. casapullo@unisa.it.
[Ti] Título:N-Formyl-7-amino-11-cycloamphilectene, a marine sponge metabolite, binds to tubulin and modulates microtubule depolymerization.
[So] Source:Mol Biosyst;10(4):862-7, 2014 Apr.
[Is] ISSN:1742-2051
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The importance of protein-small molecule interaction in drug discovery, medicinal chemistry and biology has driven the development of new analytical methods to disclose the whole interactome of bioactive compounds. To accelerate targets discovery of N-formyl-7-amino-11-cycloamphilectene (CALe), a marine bioactive diterpene isolated from the Vanuatu sponge Axinella sp., a chemoproteomic-based approach has been successfully developed. CALe is a potent anti-inflammatory agent, modulating NO and prostaglandin E2 overproduction by dual inhibition of the enhanced inducible NO synthase expression and cyclo-oxygenase-2 activity, without any evidence of cytotoxic effects. In this paper, several isoforms of tubulin have been identified as CALe off-targets by chemical proteomics combined with bio-physical orthogonal approaches. In the following biological analysis of its cellular effect, CALe was found to protect microtubules against the colcemid depolymerizing effect.
[Mh] Termos MeSH primário: Inibidores de Ciclo-Oxigenase 2/química
Diterpenos/metabolismo
Microtúbulos/metabolismo
Óxido Nítrico Sintase Tipo II/antagonistas & inibidores
Tubulina (Proteína)/metabolismo
[Mh] Termos MeSH secundário: Animais
Axinella/metabolismo
Ciclo-Oxigenase 2/biossíntese
Inibidores de Ciclo-Oxigenase 2/metabolismo
Demecolcina/farmacologia
Diterpenos/química
Óxido Nítrico Sintase Tipo II/biossíntese
Isoformas de Proteínas/efeitos dos fármacos
Proteômica
Moduladores de Tubulina/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cyclooxygenase 2 Inhibitors); 0 (Diterpenes); 0 (N-formyl-7-amino-11-cycloamphilectene); 0 (Protein Isoforms); 0 (Tubulin); 0 (Tubulin Modulators); EC 1.14.13.39 (NOS2 protein, human); EC 1.14.13.39 (Nitric Oxide Synthase Type II); EC 1.14.99.1 (Cyclooxygenase 2); EC 1.14.99.1 (PTGS2 protein, human); Z01IVE25KI (Demecolcine)
[Em] Mês de entrada:1412
[Cu] Atualização por classe:161025
[Lr] Data última revisão:
161025
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140205
[St] Status:MEDLINE
[do] DOI:10.1039/c3mb70315k


  3 / 33 MEDLINE  
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[PMID]:24268066
[Au] Autor:Haber M; Ilan M
[Ad] Endereço:Department of Zoology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel.
[Ti] Título:Diversity and antibacterial activity of bacteria cultured from Mediterranean Axinella spp. sponges.
[So] Source:J Appl Microbiol;116(3):519-32, 2014 Mar.
[Is] ISSN:1365-2672
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:AIMS: Evaluation of the diversity and antibacterial activity of bacteria cultivated from Mediterranean Axinella sponges and investigating the influence of culture conditions on antibacterial activity profiles of sponge bacteria. METHODS AND RESULTS: Based on 16S rRNA gene sequence analysis, the 259 bacteria isolated from the three Mediterranean Axinella sponges A. cannabina, A. verrucosa and A. polypoides belonged to 41 genera from the four phyla Actinobacteria, Bacteroidetes, Firmicutes and Proteobacteria and included five potential newly cultured genera. In antagonistic streak assays, 87 isolates (34%) from 13 genera showed antibacterial activity towards at least one of the 10 environmental and laboratory test bacteria. The extracts and filtrates of 22 isolates grown under three different culture conditions were less often active as the isolates in the corresponding antagonistic streak assays. Changes in antibacterial activity profiles were isolate- and culture condition-specific. CONCLUSIONS: Axinella sponges are a good source to cultivate phylogenetic diverse and hitherto novel bacteria, many of which with antibacterial activity. Analysis of induced antibacterial activities might enhance the role of sponge bacteria in efforts to isolate new antibiotics in the future. SIGNIFICANCE AND IMPACT OF THE STUDY: This study was the first to investigate the diversity and antibacterial activity of bacteria isolated from A. cannabina and A. verrucosa. It highlights the potential importance of induced activity and the need for employing multiple culture conditions in antibacterial screening assays of sponge-associated bacteria.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Axinella/microbiologia
Bactérias/classificação
[Mh] Termos MeSH secundário: Actinobacteria/isolamento & purificação
Animais
Antibacterianos/biossíntese
Bactérias/isolamento & purificação
Bactérias/metabolismo
Bacteroidetes/isolamento & purificação
Biodiversidade
Filogenia
Proteobactérias/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents)
[Em] Mês de entrada:1510
[Cu] Atualização por classe:150202
[Lr] Data última revisão:
150202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131126
[St] Status:MEDLINE
[do] DOI:10.1111/jam.12401


  4 / 33 MEDLINE  
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[PMID]:23061787
[Au] Autor:Huang R; Peng Y; Zhou X; Yang X; Liu Y
[Ad] Endereço:Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, PR China.
[Ti] Título:A new taurine derivative from South China Sea marine sponge Axinella sp.
[So] Source:Nat Prod Res;27(17):1537-41, 2013.
[Is] ISSN:1478-6427
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A new (1) and two known (2 and 3) acylated taurine derivatives were isolated from the South China Sea marine sponge Axinella sp. The structures of the compounds were determined on the basis of spectral analysis. Compounds 1-3 did not inhibit the growth of hepatoma carcinoma cell (HepG2), lung carcinoma cell (A549), human breast carcinoma (MCF-7), no-small cell lung cancer (NCI-H460) and human nasopharyngeal carcinoma cell lines.
[Mh] Termos MeSH primário: Axinella/química
Taurina/química
Taurina/farmacologia
[Mh] Termos MeSH secundário: Animais
Antineoplásicos/química
Antineoplásicos/farmacologia
Linhagem Celular Tumoral
Proliferação Celular/efeitos dos fármacos
China
Seres Humanos
Células MCF-7
Estrutura Molecular
Oceanos e Mares
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antineoplastic Agents); 1EQV5MLY3D (Taurine)
[Em] Mês de entrada:1404
[Cu] Atualização por classe:130906
[Lr] Data última revisão:
130906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:121016
[St] Status:MEDLINE
[do] DOI:10.1080/14786419.2012.733389


  5 / 33 MEDLINE  
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[PMID]:22954064
[Au] Autor:Dresch RR; Zanetti GD; Irazoqui FJ; Sendra VG; Zlocowski N; Bernardi A; Rosa RM; Battastini AM; Henriques AT; Vozári-Hampe MM
[Ad] Endereço:Postgraduation Program in Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Rio Grande do Sul, 90610-000, Porto Alegre, RS, Brazil. rrdresch@gmail.com
[Ti] Título:Staining tumor cells with biotinylated ACL-I, a lectin isolated from the marine sponge, Axinella corrugata.
[So] Source:Biotech Histochem;88(1):1-9, 2013 Jan.
[Is] ISSN:1473-7760
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Axinella corrugata lectin 1 (ACL-1) was purified from aqueous extracts of the marine sponge, Axinella corrugata. ACL-1 strongly agglutinates native rabbit erythrocytes. The hemagglutination is inhibited by N-acetyl derivatives, particularly N, N', N"-triacetylchitotriose, N-acetyl-D-glucosamine, N-acetyl-D-mannosamine and N-acetyl-D-galactosamine. We investigated the capacity of biotinylated ACL-1 to stain several transformed cell lines including breast (T-47D, MCF7), colon (HT-29), lung (H460), ovary (OVCAR-3) and bladder (T24). ACL-I may bind to both monosaccharides and oligosaccharides of tumor cells, N-acetyl-D-galactosamine, and N-acetyl-D- glucosamine glycan types. The lectins are useful, not only as markers and diagnostic parameters, but also for tissue mapping in suspicious neoplasms. In addition, they provide a better understanding of neoplasms at the cytological and molecular levels. Furthermore, the use of potential metastatic markers such as lectins is crucial for developing successful tools for therapy against cancer. We observed that biotinylated ACL-I stains tumor cells and may hold potential as a probe for identifying transformed cells and for studying glycan structures synthesized by such cells.
[Mh] Termos MeSH primário: Axinella/química
Lectinas/química
Neoplasias/química
Coloração e Rotulagem/métodos
[Mh] Termos MeSH secundário: Animais
Biotinilação
Linhagem Celular Tumoral
Cromatografia de Afinidade/métodos
Neoplasias/patologia
Coelhos
Ratos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Lectins)
[Em] Mês de entrada:1306
[Cu] Atualização por classe:121219
[Lr] Data última revisão:
121219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120908
[St] Status:MEDLINE
[do] DOI:10.3109/10520295.2012.717304


  6 / 33 MEDLINE  
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[PMID]:22904228
[Au] Autor:Haber M; Shefer S; Giordano A; Orlando P; Gambacorta A; Ilan M
[Ad] Endereço:Department of Zoology, Tel Aviv University, 69978 Tel Aviv, Israel.
[Ti] Título:Fulvitalea axinellae gen. nov., sp. nov., a member of the family Flammeovirgaceae isolated from the Mediterranean sponge Axinella verrucosa.
[So] Source:Int J Syst Evol Microbiol;63(Pt 5):1678-83, 2013 May.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The yellow-pigmented, non-motile, Gram-negative, strictly aerobic, rod-shaped bacterial strain VI.18(T) was isolated from the Mediterranean sponge Axinella verrucosa collected off the coast near Sdot Yam, Israel. Results from 16S rRNA gene sequence analysis indicated that the isolate belonged to the family Flammeovirgaceae. The highest nucleotide similarity (91.4 %) occurred with Aureibacter tunicatorum A5Q-118(T). The predominant cellular fatty acids of strain VI.18(T) were iso-C15 : 0 (56.0 %), iso-C17 : 1ω9c (22.8 %) and C16 : 0 (7.4 %) and its major respiratory quinone was MK-7. The DNA G+C content was 47.5 mol%. The strain could readily be distinguished from its phylogenetically closest relatives by phenotypic, physiological and chemotaxonomic properties. On the basis of the data from the present polyphasic study, we propose a novel genus and species within the family Flammeovirgaceae, with the name Fulvitalea axinellae gen. nov., sp. nov. Strain VI.18(T) ( = ATCC BAA-2395(T)  = LMG 26722(T)) is the type strain of Fulvitalea axinellae.
[Mh] Termos MeSH primário: Axinella/microbiologia
Bacteroidetes/classificação
Filogenia
Água do Mar/microbiologia
[Mh] Termos MeSH secundário: Animais
Técnicas de Tipagem Bacteriana
Bacteroidetes/genética
Bacteroidetes/isolamento & purificação
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/análise
Israel
Dados de Sequência Molecular
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Vitamina K 2/análogos & derivados
Vitamina K 2/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (RNA, Ribosomal, 16S); 11032-49-8 (Vitamin K 2); 8427BML8NY (vitamin MK 7)
[Em] Mês de entrada:1307
[Cu] Atualização por classe:130514
[Lr] Data última revisão:
130514
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120821
[St] Status:MEDLINE
[do] DOI:10.1099/ijs.0.044263-0


  7 / 33 MEDLINE  
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[PMID]:22707537
[Au] Autor:Haber M; Shefer S; Giordano A; Orlando P; Gambacorta A; Ilan M
[Ad] Endereço:Department of Zoology, Tel Aviv University, 69978 Tel Aviv, Israel.
[Ti] Título:Aureivirga marina gen. nov., sp. nov., a marine bacterium isolated from the Mediterranean sponge Axinella verrucosa.
[So] Source:Int J Syst Evol Microbiol;63(Pt 3):1089-95, 2013 Mar.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Two bacterial strains, VI.14 and VIII.04(T), were isolated from the Mediterranean sponge Axinella verrucosa collected off the Israeli coast near Sdot Yam. The non-motile, aerobic, Gram-negative isolates were oxidase-negative and catalase-positive, and formed golden-brown colonies on marine agar 2216. The pigment was neither diffusible nor flexirubin-like. Strain VIII.04(T) grew at 15-37 °C, at pH 6.0-9.0, in the presence of 20-50 g NaCl l(-1) and 20-80 g sea salts l(-1), The spectrum was narrower for strain VI.14, with growth at pH 7.0-8.0. and in the presence of 30-50 g NaCl l(-1) and 30-70 g sea salts l(-1). The predominant fatty acid (>50 %) in both strains was iso-C15 : 0, and the major respiratory quinone was MK-6. The DNA G+C content was 30.7 and 31.1 mol% for VIII.04(T) and VI.14, respectively. Results from 16S rRNA sequence similarity and phylogenetic analyses indicated that both strains are closely related to members of the family Flavobacteriaceae within the phylum Bacteroidetes, with as much as 91.7 % 16S rRNA sequence similarity. On the basis of data from the polyphasic analysis, we suggest that the strains represent a novel species in a new genus within the family Flavobacteriaceae, for which the name Aureivirga marina gen. nov., sp. nov. is proposed. Strain VIII.04(T) ( = ATCC BAA-2394(T) = LMG 26721(T)) is the type strain of Aureivirga marina.
[Mh] Termos MeSH primário: Axinella/microbiologia
Flavobacteriaceae/classificação
Filogenia
[Mh] Termos MeSH secundário: Animais
Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/análise
Flavobacteriaceae/genética
Flavobacteriaceae/isolamento & purificação
Dados de Sequência Molecular
Hibridização de Ácido Nucleico
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Vitamina K 2/análogos & derivados
Vitamina K 2/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (RNA, Ribosomal, 16S); 11032-49-8 (Vitamin K 2); 71ANL51TLA (menaquinone 6)
[Em] Mês de entrada:1305
[Cu] Atualização por classe:130308
[Lr] Data última revisão:
130308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120619
[St] Status:MEDLINE
[do] DOI:10.1099/ijs.0.043257-0


  8 / 33 MEDLINE  
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[PMID]:22659504
[Au] Autor:Haber M; Shefer S; Giordano A; Orlando P; Gambacorta A; Ilan M
[Ad] Endereço:Department of Zoology, Tel Aviv University, 69978 Tel Aviv, Israel.
[Ti] Título:Luteivirga sdotyamensis gen. nov., sp. nov., a novel bacterium of the phylum Bacteroidetes isolated from the Mediterranean sponge Axinella polypoides.
[So] Source:Int J Syst Evol Microbiol;63(Pt 3):939-45, 2013 Mar.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel aerobic bacterium, designated strain PIII.02(T), was isolated from a Mediterranean sponge (Axinella polypoides) collected off the Israeli coast near Sdot Yam. The non-motile cells were Gram-staining-negative, oxidase-positive and catalase-positive. The orange pigment of colonies growing on marine agar was neither diffusible nor flexirubin-like. Strain PIII.02(T) grew at 15-35 °C, at pH 6.0-9.0, with 2.0-7.0 % (w/v) NaCl, and with 1.0-8.0 % (w/v) sea salts. The predominant fatty acids were iso-C15 : 0, iso-C16 : 1 H, iso-C16 : 0, C16 : 0, anteiso-C15 : 0 and C16 : 1ω7c. The major respiratory quinone was MK-7. The genomic DNA G+C content of the novel strain was 38.1 mol%. Results from 16S rRNA gene sequence analysis indicated that strain PIII.02(T) was distantly related to established members of the phylum Bacteroidetes. The established species found to be most closely related to the novel strain was Persicobacter diffluens NCIMB 1402(T) (87.6 % 16S rRNA gene sequence similarity). Based on the phenotypic and chemotaxonomic data and the results of the phylogenetic analyses, strain PIII.02(T) represents a novel species of a new genus, for which the name Luteivirga sdotyamensis gen. nov., sp. nov. is proposed. The type strain is PIII.02(T) ( = ATCC BAA-2393(T)  = LMG 26723(T)).
[Mh] Termos MeSH primário: Axinella/microbiologia
Bacteroidetes/classificação
Filogenia
[Mh] Termos MeSH secundário: Animais
Técnicas de Tipagem Bacteriana
Bacteroidetes/genética
Bacteroidetes/isolamento & purificação
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/análise
Israel
Mar Mediterrâneo
Dados de Sequência Molecular
RNA Ribossômico 16S/genética
Água do Mar/microbiologia
Análise de Sequência de DNA
Vitamina K 2/análogos & derivados
Vitamina K 2/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (RNA, Ribosomal, 16S); 11032-49-8 (Vitamin K 2); 8427BML8NY (vitamin MK 7)
[Em] Mês de entrada:1305
[Cu] Atualização por classe:130308
[Lr] Data última revisão:
130308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120605
[St] Status:MEDLINE
[do] DOI:10.1099/ijs.0.043398-0


  9 / 33 MEDLINE  
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[PMID]:23203274
[Au] Autor:Menna M; Aiello A; D'Aniello F; Fattorusso E; Imperatore C; Luciano P; Vitalone R
[Ad] Endereço:The NeaNat Group, Department of Chemistry of Natural Products, University of Naples "Federico II", Via D. Montesano 49, Napoli 80131, Italy. mlmenna@unina.it
[Ti] Título:Further investigation of the Mediterranean sponge Axinella polypoides: isolation of a new cyclonucleoside and a new betaine.
[So] Source:Mar Drugs;10(11):2509-18, 2012 Nov 09.
[Is] ISSN:1660-3397
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:An exhaustive exploration into the metabolic content of the Mediterranean sponge Axinella-polypoides resulted in the isolation of the new betaine 5 and the new cyclonucleoside 8. The structures of the new metabolites were elucidated by spectroscopic methods assisted by computational methods. The analysis also provided evidence that the sponge does not elaborate pyrrole-imidazole alkaloids (PIAs) but, interestingly, it was shown to contain two already known cyclodipeptides, compounds 9 (verpacamide A) and 10.
[Mh] Termos MeSH primário: Axinella/química
Betaína/química
Nucleosídeos/química
Peptídeos Cíclicos/química
[Mh] Termos MeSH secundário: Animais
Betaína/isolamento & purificação
Mar Mediterrâneo
Nucleosídeos/isolamento & purificação
Peptídeos Cíclicos/isolamento & purificação
Análise Espectral
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Nucleosides); 0 (Peptides, Cyclic); 3SCV180C9W (Betaine)
[Em] Mês de entrada:1305
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:121204
[St] Status:MEDLINE
[do] DOI:10.3390/md10112509


  10 / 33 MEDLINE  
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[PMID]:22245532
[Au] Autor:Dresch RR; Lerner CB; Mothes B; Trindade VM; Henriques AT; Vozári-Hampe MM
[Ad] Endereço:Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, 90610-000, Porto Alegre, RS, Brazil. rrdresch@gmail.com
[Ti] Título:Biological activities of ACL-I and physicochemical properties of ACL-II, lectins isolated from the marine sponge Axinella corrugata.
[So] Source:Comp Biochem Physiol B Biochem Mol Biol;161(4):365-70, 2012 Apr.
[Is] ISSN:1879-1107
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Lectin II from the marine sponge Axinella corrugata (ACL-II) was purified by affinity chromatography on rabbit erythrocytic stroma incorporated into a polyacrylamide gel, followed by gel filtration on Ultrogel AcA 44 column. Purified ACL-II is a lectin with an Mr of 80 kDa and 78 kDa, estimated by SDS-PAGE and by FPLC on Superose 12 HR column, respectively. ACL-II mainly agglutinates native rabbit erythrocytes and this hemagglutinating activity is independent of Ca(2+), Mg(2+) and Mn(2+), but is inhibited by d-galactose, chitin and N-acetyl derivatives, with the exception of GalNAc. ACL-II is stable for up to 65 °C for 30 min, with a better stability at a pH range of 2 to 6. In contrast, ACL-I displays a strong mitogenic and cytotoxic effect.
[Mh] Termos MeSH primário: Axinella/química
Lectinas/metabolismo
[Mh] Termos MeSH secundário: Análise de Variância
Animais
Cromatografia de Afinidade
Cromatografia em Gel
Eletroforese em Gel de Poliacrilamida
Eritrócitos/efeitos dos fármacos
Testes de Hemaglutinação
Seres Humanos
Concentração de Íons de Hidrogênio
Lectinas/isolamento & purificação
Lectinas/farmacologia
Coelhos
Corantes de Rosanilina
Fatores de Tempo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Lectins); 0 (Rosaniline Dyes); M1ZRX790SI (coomassie Brilliant Blue)
[Em] Mês de entrada:1206
[Cu] Atualização por classe:160906
[Lr] Data última revisão:
160906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120117
[St] Status:MEDLINE
[do] DOI:10.1016/j.cbpb.2012.01.001



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