Base de dados : MEDLINE
Pesquisa : B01.050.500.802.175 [Categoria DeCS]
Referências encontradas : 8 [refinar]
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  1 / 8 MEDLINE  
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[PMID]:26225985
[Au] Autor:Roel M; Rubiolo JA; Ternon E; Thomas OP; Vieytes MR; Botana LM
[Ad] Endereço:Departamento de Farmacología, Facultad de Veterinaria, Universidad de Santiago de Compostela (USC), Campus Lugo, 27002 Lugo, Spain. maria.roel@usc.es.
[Ti] Título:Crambescin C1 Exerts a Cytoprotective Effect on HepG2 Cells through Metallothionein Induction.
[So] Source:Mar Drugs;13(8):4633-53, 2015 Jul 27.
[Is] ISSN:1660-3397
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The Mediterranean marine sponge Crambe crambe is the source of two families of guanidine alkaloids known as crambescins and crambescidins. Some of the biological effects of crambescidins have been previously reported while crambescins have undergone little study. Taking this into account, we performed comparative transcriptome analysis to examine the effect of crambescin-C1 (CC1) on human tumor hepatocarcinoma cells HepG2 followed by validation experiments to confirm its predicted biological activities. We report herein that, while crambescin-A1 has a minor effect on these cells, CC1 protects them against oxidative injury by means of metallothionein induction even at low concentrations. Additionally, at high doses, CC1 arrests the HepG2 cell cycle in G0/G1 and thus inhibits tumor cell proliferation. The findings presented here provide the first detailed approach regarding the different effects of crambescins on tumor cells and provide a basis for future studies on other possible cellular mechanisms related to these bioactivities.
[Mh] Termos MeSH primário: Fatores Biológicos/farmacologia
Citoproteção/efeitos dos fármacos
Metalotioneína/metabolismo
Pirimidinas/farmacologia
[Mh] Termos MeSH secundário: Alcaloides/farmacologia
Animais
Linhagem Celular Tumoral
Proliferação Celular/efeitos dos fármacos
Crambe (Esponja)/metabolismo
Fase G1/efeitos dos fármacos
Células Hep G2
Seres Humanos
Fase de Repouso do Ciclo Celular/efeitos dos fármacos
Transcriptoma/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Alkaloids); 0 (Biological Factors); 0 (Pyrimidines); 142605-06-9 (crambescin C1); 9038-94-2 (Metallothionein)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150731
[St] Status:MEDLINE
[do] DOI:10.3390/md13084633


  2 / 8 MEDLINE  
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[PMID]:24419288
[Au] Autor:Pérez-López P; Ternon E; González-García S; Genta-Jouve G; Feijoo G; Thomas OP; Moreira MT
[Ad] Endereço:Department of Chemical Engineering, School of Engineering, University of Santiago de Compostela, 15782, Santiago de Compostela, Spain. Electronic address: paula.perez@usc.es.
[Ti] Título:Environmental solutions for the sustainable production of bioactive natural products from the marine sponge Crambe crambe.
[So] Source:Sci Total Environ;475:71-82, 2014 Mar 15.
[Is] ISSN:1879-1026
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Crambe crambe is a Mediterranean marine sponge known to produce original natural substances belonging to two families of guanidine alkaloids, namely crambescins and crambescidins, which exhibit cytotoxic and antiviral activities. These compounds are therefore considered as potential anticancer drugs. The present study focuses on the environmental assessment of a novel in vivo process for the production of pure crambescin and crambescidin using sponge specimens cultured in aquarium. The assessment was performed following the ISO 14040 standard and extended from the production of the different mass and energy flows to the system to the growth of the sponge in indoor aquarium and further periodic extraction and purification of the bioactive compounds. According to the results, the two stages that have a remarkable contribution to all impact categories are the purification of the bioactive molecules followed by the maintenance of the sponge culture in the aquarium. Among the involved activities, the production of the chemicals (particularly methanol) together with the electricity requirements (especially due to the aquarium lighting) are responsible for up to 90% of the impact in most of the assessed categories. However, the contributions of other stages to the environmental burdens, such as the collection of sponges, considerably depend on the assumptions made during the inventory stage. The simulation of alternative scenarios has led to propose improvement alternatives that may allow significant reductions ranging from 20% to 70%, mainly thanks to the reduction of electricity requirements as well as the partial reuse of methanol.
[Mh] Termos MeSH primário: Alcaloides/análise
Produtos Biológicos/análise
Conservação dos Recursos Naturais/métodos
Crambe (Esponja)/crescimento & desenvolvimento
Pesqueiros/métodos
Guanidinas/análise
[Mh] Termos MeSH secundário: Animais
Crambe (Esponja)/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Alkaloids); 0 (Biological Products); 0 (Guanidines)
[Em] Mês de entrada:1409
[Cu] Atualização por classe:140205
[Lr] Data última revisão:
140205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140115
[St] Status:MEDLINE


  3 / 8 MEDLINE  
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[PMID]:24071658
[Au] Autor:Öztürk B; de Jaeger L; Smidt H; Sipkema D
[Ad] Endereço:Wageningen University, Laboratory of Microbiology, Dreijenplein 10, 6703 HB Wageningen, The Netherlands.
[Ti] Título:Culture-dependent and independent approaches for identifying novel halogenases encoded by Crambe crambe (marine sponge) microbiota.
[So] Source:Sci Rep;3:2780, 2013 Sep 27.
[Is] ISSN:2045-2322
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Sponges harbour microbial communities that contribute to the genetic and metabolic potential of their host. Among metabolites produced by sponge-associated microbial communities, halogenated compounds are of special interest because of their biotechnological potential. In this study, we have examined the diversity of the cultivable fraction of the marine demosponge Crambe crambe microbiota. Application of complementary cultivation methods yielded 107 bacterial isolates, some of which may be sponge-specific based on their phylogenetic analysis. Among these, Psychrobacter sp. was found to contain a putative halogenase gene. In addition to the culture-dependent approach for discovering halogenase genes, a cDNA library was constructed to determine the diversity of halogenase genes expressed in situ by the C. crambe microbiota. To this end, seventeen putative tryptophan halogenase cDNA sequences were identified, most of which were only remotely related to known halogenase genes, indicating the potential for novel bioactive compounds being produced by the C. crambe microbiota.
[Mh] Termos MeSH primário: Bactérias/genética
Crambe (Esponja)/microbiologia
Microbiota
[Mh] Termos MeSH secundário: Animais
Bactérias/classificação
Bactérias/enzimologia
Variação Genética
Dados de Sequência Molecular
Oxirredutases/genética
Filogenia
RNA Bacteriano
RNA Ribossômico 16S
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Bacterial); 0 (RNA, Ribosomal, 16S); EC 1.- (Oxidoreductases); EC 1.- (tryptophan halogenase)
[Em] Mês de entrada:1406
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130928
[St] Status:MEDLINE
[do] DOI:10.1038/srep02780


  4 / 8 MEDLINE  
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[PMID]:24002533
[Au] Autor:Croué J; West NJ; Escande ML; Intertaglia L; Lebaron P; Suzuki MT
[Ad] Endereço:1] UPMC Univ. Paris 06, UMR 7621, LOMIC, UMR 7232, BIOM, UMS 2348 (Plate-forme Bio2Mar), Observatoire Océanologique, F-66650 Banyuls-sur-Mer, France [2] CNRS, UMR 7621, LOMIC, F-66650, Observatoire Océanologique, Banyuls-sur-Mer, France.
[Ti] Título:A single betaproteobacterium dominates the microbial community of the crambescidine-containing sponge Crambe crambe.
[So] Source:Sci Rep;3:2583, 2013.
[Is] ISSN:2045-2322
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Crambe crambe is a marine sponge that produces high concentrations of the pharmacologically significant pentacyclic guanidine alkaloids (PGAs), Crambescines and Crambescidines. Although bio-mimetic chemical synthesis of PGAs suggests involvement of microorganisms in their biosynthesis, there are conflicting reports on whether bacteria are associated with this sponge or not. Using 16S rRNA gene pyrosequencing we show that the associated bacterial community of C. crambe is dominated by a single bacterial species affiliated to the Betaproteobacteria. Microscopy analysis of sponge tissue sections using a specific probe and in situ hybridization confirmed its dominance in the sponge mesohyl and a single microbial morphology was observed by transmission electron microscopy. If confirmed the presence of a simple bacteria community in C. crambe makes this association a very pertinent model to study sponge-bacteria interactions and should allow further research into the possible implication of bacteria in PGA biosynthesis.
[Mh] Termos MeSH primário: Betaproteobacteria/fisiologia
Crambe (Esponja)/metabolismo
Crambe (Esponja)/microbiologia
Microbiota
Compostos de Espiro/metabolismo
[Mh] Termos MeSH secundário: Animais
Betaproteobacteria/classificação
Betaproteobacteria/genética
Biodiversidade
Crambe (Esponja)/ultraestrutura
Filogenia
RNA Ribossômico 16S
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S); 0 (Spiro Compounds)
[Em] Mês de entrada:1406
[Cu] Atualização por classe:150423
[Lr] Data última revisão:
150423
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130905
[St] Status:MEDLINE
[do] DOI:10.1038/srep02583


  5 / 8 MEDLINE  
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[PMID]:21544815
[Au] Autor:Ottinger S; Klöppel A; Rausch V; Liu L; Kallifatidis G; Gross W; Gebhard MM; Brümmer F; Herr I
[Ad] Endereço:Molecular OncoSurgery, University of Heidelberg and German Cancer Research Center, Heidelberg, Germany.
[Ti] Título:Targeting of pancreatic and prostate cancer stem cell characteristics by Crambe crambe marine sponge extract.
[So] Source:Int J Cancer;130(7):1671-81, 2012 Apr 01.
[Is] ISSN:1097-0215
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cancer stem cells (CSCs) are suggested as reason for resistance of tumors toward conventional tumor therapy including pancreatic and advanced prostate cancer. New therapeutic agents are urgently needed for targeting of CSCs. Marine sponges harbor novel and undefined compounds with antineoplastic activity but their potential to eliminate CSC characteristics is not examined so far. We collected 10 marine sponges and one freshwater sponge by diving at the seaside and prepared crude methanolic extracts. The effect to established pancreatic and prostate CSC lines was evaluated by analysis of apoptosis, cell cycle, side population, colony and spheroid formation, migratory potential in vitro and tumorigenicity in vivo. While each sponge extract at a 1:10 dilution efficiently diminished viability, Crambe crambe marine sponge extract (CR) still strongly reduced viability of tumor cells at a dilution of 1:1,000 but was less toxic to normal fibroblasts and endothelial cells. CR inhibited self-renewal capacity, apoptosis resistance, and proliferation even in gemcitabine-selected pancreatic cancer cells with acquired therapy resistance and enhanced CSC characteristics. CR pretreatment of tumor cells diminished tumorigenicity of gemcitabine-resistant tumor cells in mice and totally abolished tumor take upon combination with gemcitabine. Our data suggest that CR contains substances, which render standard cancer therapy more effective by targeting of CSC characteristics. Isolation of bioactive metabolites from CR and evaluation in mice are required for development of new CSC-specific chemotherapeutic drugs from a marine sponge.
[Mh] Termos MeSH primário: Protocolos de Quimioterapia Combinada Antineoplásica/farmacologia
Crambe (Esponja)/química
Células-Tronco Neoplásicas/efeitos dos fármacos
Neoplasias Pancreáticas/tratamento farmacológico
Neoplasias da Próstata/tratamento farmacológico
[Mh] Termos MeSH secundário: Animais
Apoptose/efeitos dos fármacos
Caspases/metabolismo
Ciclo Celular/efeitos dos fármacos
Morte Celular/efeitos dos fármacos
Linhagem Celular Tumoral
Movimento Celular/efeitos dos fármacos
Proliferação Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Transformação Celular Neoplásica/efeitos dos fármacos
Células Cultivadas
Desoxicitidina/administração & dosagem
Desoxicitidina/análogos & derivados
Células Endoteliais/efeitos dos fármacos
Células Endoteliais/metabolismo
Feminino
Fibroblastos/efeitos dos fármacos
Fibroblastos/metabolismo
Seres Humanos
Masculino
Camundongos
Camundongos Nus
Células-Tronco Neoplásicas/metabolismo
Células-Tronco Neoplásicas/patologia
Neoplasias Pancreáticas/metabolismo
Neoplasias Pancreáticas/patologia
Neoplasias da Próstata/metabolismo
Neoplasias da Próstata/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0W860991D6 (Deoxycytidine); B76N6SBZ8R (gemcitabine); EC 3.4.22.- (Caspases)
[Em] Mês de entrada:1206
[Cu] Atualização por classe:160303
[Lr] Data última revisão:
160303
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110506
[St] Status:MEDLINE
[do] DOI:10.1002/ijc.26168


  6 / 8 MEDLINE  
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[PMID]:17196674
[Au] Autor:Cebrian E; Uriz MJ
[Ad] Endereço:Centre d'Estudis Avançats de Blanes, CSIC. C/Accés a la Cala St. Francesc, 14, 17300 Blanes, Girona, Spain. emma@ceab.csic.es
[Ti] Título:Contrasting effects of heavy metals and hydrocarbons on larval settlement and juvenile survival in sponges.
[So] Source:Aquat Toxicol;81(2):137-43, 2007 Feb 28.
[Is] ISSN:0166-445X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Metals and polycyclic aromatic hydrocarbons (PAHs) contaminate sediments and waters of coastal areas threatening early stages of invertebrate development. Effects on these stages may largely determine the decline and even disappearance of invertebrate populations in polluted environments. Our study aimed to determine the possible influence of metals (Cu and Cd) and PAHs on larval settlement and consecutive survival of two widespread sponges of the Mediterranean: Crambe crambe and Scopalina lophyropoda. Larvae of both species were exposed to Cu and Cd for a short period during 1 week, and settlement and following (6 months) survival of juvenile were monitored. Short exposures to copper and cadmium at the concentrations used did not affect C. crambe settlement compared with SW control, and no effect on consecutive survival of juveniles was observed. In contrast, short pulses of copper and cadmium at the concentrations used enhanced Scopalina lophyropoda settlement and did not affect the consecutive survival of juveniles with respect to SW controls. Furthermore, experiments designed to assess the effects of short exposures to PAHs and the combined effect of contamination by Cu(2+) and PAHs on larval settlement, were conduced during 10 days on C. crambe larvae. Hydrocarbons, differently than copper and cadmium, inhibited the settlement of sponge larvae to a certain extent. The synergetic negative effect of copper and hydrocarbons on C. crambe settlers may cause a decline of populations in areas with both sources of contamination. The present study provides the only available data on toxicity of copper, cadmium and hydrocarbon toxicants on sponge larval settlement.
[Mh] Termos MeSH primário: Cádmio/toxicidade
Cobre/toxicidade
Hidrocarbonetos Aromáticos Policíclicos/toxicidade
Poríferos/efeitos dos fármacos
Poluentes Químicos da Água/toxicidade
[Mh] Termos MeSH secundário: Animais
Crambe (Esponja)/efeitos dos fármacos
Larva/efeitos dos fármacos
Mar Mediterrâneo
Análise de Sobrevida
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Polycyclic Aromatic Hydrocarbons); 0 (Water Pollutants, Chemical); 00BH33GNGH (Cadmium); 789U1901C5 (Copper)
[Em] Mês de entrada:0704
[Cu] Atualização por classe:161124
[Lr] Data última revisão:
161124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:070102
[St] Status:MEDLINE


  7 / 8 MEDLINE  
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[PMID]:16862426
[Au] Autor:Garcia Camacho F; Chileh T; Cerón García MC; Sánchez Mirón A; Belarbi EH; Chisti Y; Molina Grima E
[Ad] Endereço:Department of Chemical Engineering, University of Almería, 04120 Almería, Spain. fgarcia@ual.es
[Ti] Título:A bioreaction-diffusion model for growth of marine sponge explants in bioreactors.
[So] Source:Appl Microbiol Biotechnol;73(3):525-32, 2006 Dec.
[Is] ISSN:0175-7598
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Marine sponges are sources of high-value bioactives. Engineering aspects of in vitro culture of sponges from cuttings (explants) are poorly understood. This work develops a diffusion-controlled growth model for sponge explants. The model assumes that the explant growth is controlled by diffusive transport of at least some nutrients from the surrounding medium into the explant that generally has a poorly developed aquiferous system for internal irrigation during early stages of growth. Growth is assumed to obey Monod-type kinetics. The model is shown to satisfactorily explain the measured growth behavior of the marine sponge Crambe crambe in two different growth media. In addition, the model is generally consistent with published data for growth of explants of the sponges Disidea avara and Hemimycale columella. The model predicted that nutrient concentration profiles for nutrients, such as dissolved oxygen within the explant, are consistent with data published by independent researchers. In view of the proposed model's ability to explain available data for growth of several species of sponge explants, diffusive transport does play a controlling role in explant growth at least until a fully developed aquiferous system has become established. According to the model and experimental observations, the instantaneous growth rate depends on the size of the explant and all those factors that influence the diffusion of critical nutrients within the explant. Growth follows a hyperbolic profile that is consistent with the Monod kinetics.
[Mh] Termos MeSH primário: Reatores Biológicos
Crambe (Esponja)/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Animais
Crambe (Esponja)/metabolismo
Meios de Cultura/química
Difusão
Cinética
Modelos Biológicos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Culture Media)
[Em] Mês de entrada:0701
[Cu] Atualização por classe:061122
[Lr] Data última revisão:
061122
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:060725
[St] Status:MEDLINE


  8 / 8 MEDLINE  
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[PMID]:16739962
[Au] Autor:Garcia Camacho F; Chileh T; Cerón García MC; Sanchez Mirón A; Belarbi EH; Contreras Gómez A; Molina Grima E
[Ad] Endereço:Department of Chemical Engineering, University of Almería, 04120 Almería, Spain. fgarcia@ual.es
[Ti] Título:Sustained growth of explants from Mediterranean sponge Crambe crambe cultured in vitro with enriched RPMI 1640.
[So] Source:Biotechnol Prog;22(3):781-90, 2006 May-Jun.
[Is] ISSN:8756-7938
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Marine sponges are potential sources of many unique metabolites, including cytotoxic and anticancer compounds. Natural sponge populations are insufficient or inaccessible for producing commercial quantities of metabolites of interest. It is commonly accepted that tissue (fragments, explants, and primmorphs) and in vitro cell cultivation show great potential. However, there is little knowledge of the nutritional requirements of marine sponges to carry out efficient and sustained in vitro culture and progress has been slow. In marine invertebrate fila many unsuccessful attempts have been made with in vitro cultures using typical commercial animal cell media based on sources of dissolved organic carbon (DOC) (e.g., DMEM, RPMI, M199, L-15, etc.). One of the reasons for this failure is the use of hardly identifiable growth promoters, based on terrestrial animal sera. An alternative is the use of extracts from marine animals, since they may contain nutrients necessary for growth. In this work we have cultivated in vitro explants of the encrusting marine sponge Crambe crambe. It is one of the most abundant sponges on the Mediterranean coastline and also possesses an array of potentially active metabolites (crambines and crambescidins). Initially a new approach was developed in order to show consumption of DOC by explants. Thus, different initial DOC concentrations (300, 400, 700 and 1200 mg DOC L(-1)) were assayed. Consumption was evident in all four assays and was more marked in the first 6 h. The DOC assimilation data were adjusted to an empirical model widely used for uptake kinetics of organic dissolved compounds in marine invertebrates. Second, a protocol was established to cultivate explants in vitro. Different medium formulations based on RPMI 1640 commercial medium enriched with amino acids and inorganic salts to emulate seawater salinity were assayed. The enrichment of this medium with an Octopus aqueous extract in the proportions of 10% and 20% (v/v) resulted in an evident sustained long-term growth of C. crambe explants. This growth enhancement produced high metabolic activity in the explants, as is confirmed by the high ammonium and lactate content in the medium a few days after its renewal and by the consumption of glucose. The lactate accumulation increased with the size and age of explants. Prior to these experiments, we successfully developed a robust new alternative method, based on digital image treatment, for accurate determination of the explant apparent volume as growth measure.
[Mh] Termos MeSH primário: Crambe (Esponja)/efeitos dos fármacos
Crambe (Esponja)/crescimento & desenvolvimento
Meios de Cultura/farmacologia
[Mh] Termos MeSH secundário: Animais
Técnicas de Cultura de Células/métodos
Células Cultivadas
Crambe (Esponja)/metabolismo
Relação Dose-Resposta a Droga
Cinética
Mar Mediterrâneo
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Culture Media)
[Em] Mês de entrada:0608
[Cu] Atualização por classe:141120
[Lr] Data última revisão:
141120
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:060603
[St] Status:MEDLINE



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