Base de dados : MEDLINE
Pesquisa : B01.050.500.802.360 [Categoria DeCS]
Referências encontradas : 27 [refinar]
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  1 / 27 MEDLINE  
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[PMID]:27100857
[Au] Autor:Olsen EK; Søderholm KL; Isaksson J; Andersen JH; Hansen E
[Ad] Endereço:MabCent-SFI, ‡Department of Chemistry, and §Marbio, UiT The Arctic University of Norway , N-9037 Tromsø, Norway.
[Ti] Título:Metabolomic Profiling Reveals the N-Acyl-Taurine Geodiataurine in Extracts from the Marine Sponge Geodia macandrewii (Bowerbank).
[So] Source:J Nat Prod;79(5):1285-91, 2016 May 27.
[Is] ISSN:1520-6025
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A metabolomic approach was used to identify known and new natural products from the marine sponges Geodia baretti and G. macandrewii. G. baretti is known to produce bioactive natural products such as barettin (1), 8,9-dihydrobarettin (2), and bromobenzisoxazolone barettin (3), while secondary metabolites from G. macandrewii are not reported in the literature. Specimens of the two sponges were collected from different sites along the coast of Norway, and their extracts were analyzed using UHPLC-HR-MS. Metabolomic analyses revealed that extracts from both species contained barettin (1) and 8,9-dihydrobarettin (2), and all samples of G. baretti contained higher amounts of both compounds compared to G. macandrewii. The analysis of the MS data also revealed that samples of G. macandrewii contained a compound that was not present in any of the G. baretti samples. This new compound was isolated and identified as the N-acyl-taurine geodiataurine (4), and it was tested for antioxidant, anticancer, and antibacterial properties.
[Mh] Termos MeSH primário: Geodia/química
Taurina/análise
[Mh] Termos MeSH secundário: Animais
Produtos Biológicos/isolamento & purificação
Biologia Marinha
Metabolômica
Testes de Sensibilidade Microbiana
Estrutura Molecular
Noruega
Ressonância Magnética Nuclear Biomolecular
Peptídeos Cíclicos/química
Peptídeos Cíclicos/isolamento & purificação
Peptídeos Cíclicos/farmacologia
Taurina/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (8,9-dihydrobarettin); 0 (Biological Products); 0 (Peptides, Cyclic); 0 (barettin); 0 (bromobenzisoxazolone barettin); 1EQV5MLY3D (Taurine)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170605
[Lr] Data última revisão:
170605
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160422
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jnatprod.5b00966


  2 / 27 MEDLINE  
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[PMID]:26970856
[Au] Autor:Edge KJ; Johnston EL; Dafforn KA; Simpson SL; Kutti T; Bannister RJ
[Ad] Endereço:Evolution and Ecology Research Centre, School of Biological and Environmental Sciences, University of New South Wales, Sydney, NSW 2052, Australia. Electronic address: katelyn.edge@environment.nsw.gov.au.
[Ti] Título:Sub-lethal effects of water-based drilling muds on the deep-water sponge Geodia barretti.
[So] Source:Environ Pollut;212:525-534, 2016 May.
[Is] ISSN:1873-6424
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Offshore oil and gas activities can result in the discharge of large amounts of drilling muds. While these materials have generally been regarded as non-toxic to marine organisms, recent studies have demonstrated negative impacts to suspension feeding organisms. We exposed the arctic-boreal sponge Geodia barretti to the primary particulate components of two water-based drilling muds; barite and bentonite. Sponges were exposed to barite, bentonite and a natural reference sediment at a range of total suspended solid concentrations (TSS = 0, 10, 50 or 100 mg/L) for 12 h after which we measured a suite of biomarker responses (lysosomal membrane stability, lipid peroxidation and glutathione). In addition, we compared biomarker responses, organic energy content and metal accumulation in sponges, which had been continuously or intermittently exposed to suspended barite and natural sediment for 14 d at relevant concentrations (10 and 30 mg TSS/L). Lysosomal membrane stability was reduced in the sponges exposed to barite at 50 and 100 mg TSS/L after just 12 h and at 30 mg TSS/L for both continuous and intermittent exposures over 14 d. Evidence of compromised cellular viability was accompanied by barite analysis revealing concentrations of Cu and Pb well above reference sediments and Norwegian sediment quality guidelines. Metal bioaccumulation in sponge tissues was low and the total organic energy content (determined by the elemental composition of organic tissue) was not affected. Intermittent exposures to barite resulted in less toxicity than continuous exposure to barite. Short term exposures to bentonite did not alter any biomarker responses. This is the first time that these biomarkers have been used to indicate contaminant exposure in an arctic-boreal sponge. Our results illustrate the potential toxicity of barite and the importance of assessments that reflect the ways in which these contaminants are delivered under environmentally realistic conditions.
[Mh] Termos MeSH primário: Geodia/efeitos dos fármacos
Sedimentos Geológicos/química
Poluentes Químicos da Água/toxicidade
[Mh] Termos MeSH secundário: Animais
Sulfato de Bário
Bentonita
Indústrias Extrativas e de Processamento
Resíduos Industriais
Lisossomos
Metais/química
Metais/metabolismo
Noruega
Poluentes Químicos da Água/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Industrial Waste); 0 (Metals); 0 (Water Pollutants, Chemical); 1302-78-9 (Bentonite); 25BB7EKE2E (Barium Sulfate)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170904
[Lr] Data última revisão:
170904
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160314
[St] Status:MEDLINE


  3 / 27 MEDLINE  
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[PMID]:26695619
[Au] Autor:Olsen EK; Hansen E; W K Moodie L; Isaksson J; Sepcic K; Cergolj M; Svenson J; Andersen JH
[Ad] Endereço:MabCent-SFI, UiT The Arctic University of Norway, Breivika, N-9037, Tromsø, Norway.
[Ti] Título:Marine AChE inhibitors isolated from Geodia barretti: natural compounds and their synthetic analogs.
[So] Source:Org Biomol Chem;14(5):1629-40, 2016 Feb 07.
[Is] ISSN:1477-0539
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Barettin, 8,9-dihydrobarettin, bromoconicamin and a novel brominated marine indole were isolated from the boreal sponge Geodia barretti collected off the Norwegian coast. The compounds were evaluated as inhibitors of electric eel acetylcholinesterase. Barettin and 8,9-dihydrobarettin displayed significant inhibition of the enzyme, with inhibition constants (Ki) of 29 and 19 µM respectively towards acetylcholinesterase via a reversible noncompetitive mechanism. These activities are comparable to those of several other natural acetylcholinesterase inhibitors of marine origin. Bromoconicamin was less potent against acetylcholinesterase, and the novel compound was inactive. Based on the inhibitory activity, a library of 22 simplified synthetic analogs was designed and prepared to probe the role of the brominated indole, common to all the isolated compounds. From the structure-activity investigation it was shown that the brominated indole motif is not sufficient to generate a high acetylcholinesterase inhibitory activity, even when combined with natural cationic ligands for the acetylcholinesterase active site. The four natural compounds were also analysed for their butyrylcholinesterase inhibitory activity in addition and shown to display comparable activities. The study illustrates how both barettin and 8,9-dihydrobarettin display additional bioactivities which may help to explain their biological role in the producing organism. The findings also provide new insights into the structure-activity relationship of both natural and synthetic acetylcholinesterase inhibitors.
[Mh] Termos MeSH primário: Acetilcolinesterase/metabolismo
Produtos Biológicos/farmacologia
Inibidores da Colinesterase/farmacologia
Geodia/química
[Mh] Termos MeSH secundário: Animais
Produtos Biológicos/química
Produtos Biológicos/isolamento & purificação
Inibidores da Colinesterase/química
Inibidores da Colinesterase/isolamento & purificação
Seres Humanos
Alcaloides de Indol/química
Alcaloides de Indol/isolamento & purificação
Alcaloides de Indol/farmacologia
Indóis/química
Indóis/isolamento & purificação
Indóis/farmacologia
Peptídeos Cíclicos/química
Peptídeos Cíclicos/isolamento & purificação
Peptídeos Cíclicos/farmacologia
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (8,9-dihydrobarettin); 0 (Biological Products); 0 (Cholinesterase Inhibitors); 0 (Indole Alkaloids); 0 (Indoles); 0 (Peptides, Cyclic); 0 (barettin); 0 (conicamin); EC 3.1.1.7 (Acetylcholinesterase)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151224
[St] Status:MEDLINE
[do] DOI:10.1039/c5ob02416a


  4 / 27 MEDLINE  
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[PMID]:26491222
[Au] Autor:Costantini S; Romano G; Rusolo F; Capone F; Guerriero E; Colonna G; Ianora A; Ciliberto G; Costantini M
[Ad] Endereço:CROM, Istituto Nazionale Tumori "Fondazione G. Pascale", IRCCS, 80131 Napoli, Italy.
[Ti] Título:Anti-Inflammatory Effects of a Methanol Extract from the Marine Sponge Geodia cydonium on the Human Breast Cancer MCF-7 Cell Line.
[So] Source:Mediators Inflamm;2015:204975, 2015.
[Is] ISSN:1466-1861
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Many research groups are working to find new possible anti-inflammatory molecules, and marine sponges represent a rich source of biologically active compounds with pharmacological applications. In the present study, we tested different concentrations of the methanol extract from the marine sponge, Geodia cydonium, on normal human breast epithelial cells (MCF-10A) and human breast cancer cells (MCF-7). Our results show that this extract has no cytotoxic effects on both cell lines whereas it induces a decrease in levels of VEGF and five proinflammatory cytokines (CCL2, CXCL8, CXCL10, IFN-γ, and TNF-α) only in MCF-7 cells in a dose-dependent manner, thereby indicating an anti-inflammatory effect. Moreover, interactomic analysis suggests that all six cytokines are involved in a network and are connected with some HUB nodes such as NF-kB subunits and ESR1 (estrogen receptor 1). We also report a decrease in the expression of two NFKB1 and c-Rel subunits by RT-qPCR experiments only in MCF-7 cells after extract treatment, confirming NF-kB inactivation. These data highlight the potential of G. cydonium for future drug discovery against major diseases, such as breast cancer.
[Mh] Termos MeSH primário: Geodia/química
Metanol/química
[Mh] Termos MeSH secundário: Animais
Neoplasias da Mama/metabolismo
Sobrevivência Celular/efeitos dos fármacos
Feminino
Seres Humanos
Células MCF-7
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
Y4S76JWI15 (Methanol)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151023
[St] Status:MEDLINE
[do] DOI:10.1155/2015/204975


  5 / 27 MEDLINE  
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[PMID]:26222779
[Au] Autor:Carstens BB; Rosengren KJ; Gunasekera S; Schempp S; Bohlin L; Dahlström M; Clark RJ; Göransson U
[Ti] Título:Isolation, Characterization, and Synthesis of the Barrettides: Disulfide-Containing Peptides from the Marine Sponge Geodia barretti.
[So] Source:J Nat Prod;78(8):1886-93, 2015 Aug 28.
[Is] ISSN:1520-6025
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Two disulfide-containing peptides, barrettides A (1) and B (2), from the cold-water marine sponge Geodia barretti are described. Those 31 amino acid residue long peptides were sequenced using mass spectrometry methods and structurally characterized using NMR spectroscopy. The structure of 1 was confirmed by total synthesis using the solid-phase peptide synthesis approach that was developed. The two peptides were found to differ only at a single position in their sequence. The three-dimensional structure of 1 revealed that these peptides possess a unique fold consisting of a long ß-hairpin structure that is cross-braced by two disulfide bonds in a ladder-like arrangement. The peptides are amphipathic in nature with the hydrophobic and charged residues clustered on separate faces of the molecule. The barrettides were found not to inhibit the growth of either Escherichia coli or Staphylococcus aureus but displayed antifouling activity against barnacle larvae (Balanus improvisus) without lethal effects in the concentrations tested.
[Mh] Termos MeSH primário: Dissulfetos/química
Geodia/química
Peptídeos Cíclicos/isolamento & purificação
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Incrustação Biológica/prevenção & controle
Temperatura Baixa
Larva/efeitos dos fármacos
Biologia Marinha
Estrutura Molecular
Ressonância Magnética Nuclear Biomolecular
Peptídeos Cíclicos/química
Conformação Proteica
Técnicas de Síntese em Fase Sólida
Staphylococcus aureus/efeitos dos fármacos
Thoracica/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Disulfides); 0 (Peptides, Cyclic); 0 (barrettide A); 0 (barrettide B)
[Em] Mês de entrada:1511
[Cu] Atualização por classe:150828
[Lr] Data última revisão:
150828
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150730
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jnatprod.5b00210


  6 / 27 MEDLINE  
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[PMID]:24324768
[Au] Autor:Knudby A; Kenchington E; Murillo FJ
[Ad] Endereço:Department of Geography, Simon Fraser University, Burnaby, British Columbia, Canada.
[Ti] Título:Modeling the distribution of Geodia sponges and sponge grounds in the Northwest Atlantic.
[So] Source:PLoS One;8(12):e82306, 2013.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Deep-sea sponge grounds provide structurally complex habitat for fish and invertebrates and enhance local biodiversity. They are also vulnerable to bottom-contact fisheries and prime candidates for Vulnerable Marine Ecosystem designation and related conservation action. This study uses species distribution modeling, based on presence and absence observations of Geodia spp. and sponge grounds derived from research trawl catches, as well as spatially continuous data on the physical and biological ocean environment derived from satellite data and oceanographic models, to model the distribution of Geodia sponges and sponge grounds in the Northwest Atlantic. Most models produce excellent fits with validation data although fits are reduced when models are extrapolated to new areas, especially when oceanographic regimes differ between areas. Depth and minimum bottom salinity were important predictors in most models, and a Geodia spp. minimum bottom salinity tolerance threshold in the 34.3-34.8 psu range was hypothesized on the basis of model structure. The models indicated two currently unsampled regions within the study area, the deeper parts of Baffin Bay and the Newfoundland and Labrador slopes, where future sponge grounds are most likely to be found.
[Mh] Termos MeSH primário: Geodia/fisiologia
Modelos Biológicos
[Mh] Termos MeSH secundário: Animais
Área Sob a Curva
Oceano Atlântico
Clorofila/análise
Geografia
Probabilidade
Salinidade
Temperatura Ambiente
Movimentos da Água
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
1406-65-1 (Chlorophyll); YF5Q9EJC8Y (chlorophyll a)
[Em] Mês de entrada:1409
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131211
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0082306


  7 / 27 MEDLINE  
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[PMID]:23880935
[Au] Autor:Lind KF; Hansen E; Østerud B; Eilertsen KE; Bayer A; Engqvist M; Leszczak K; Jørgensen TØ; Andersen JH
[Ad] Endereço:MabCent-SFI, University of Tromsø, Breivika N-9037 Tromsø, Norway. karianne.lind@uit.no
[Ti] Título:Antioxidant and anti-inflammatory activities of barettin.
[So] Source:Mar Drugs;11(7):2655-66, 2013 Jul 22.
[Is] ISSN:1660-3397
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:In this paper, we present novel bioactivity for barettin isolated from the marine sponge Geodia barretti. We found that barettin showed strong antioxidant activity in biochemical assays as well as in a lipid peroxidation cell assay. A de-brominated synthetic analogue of barettin did not show the same activity in the antioxidant cell assay, indicating that bromine is important for cellular activity. Barettin was also able to inhibit the secretion of the inflammatory cytokines IL-1ß and TNFα from LPS-stimulated THP-1 cells. This combination of anti-inflammatory and antioxidant activities could indicate that barettin has an atheroprotective effect and may therefore be an interesting product to prevent development of atherosclerosis.
[Mh] Termos MeSH primário: Anti-Inflamatórios/farmacologia
Antioxidantes/farmacologia
Peptídeos Cíclicos/farmacologia
[Mh] Termos MeSH secundário: Animais
Anti-Inflamatórios/química
Antioxidantes/química
Fatores Biológicos/química
Fatores Biológicos/farmacologia
Bromo/metabolismo
Linhagem Celular Tumoral
Geodia/química
Células Hep G2
Seres Humanos
Interleucina-1beta/metabolismo
Biologia Marinha
Peptídeos Cíclicos/química
Poríferos/química
Poríferos/metabolismo
Fator de Necrose Tumoral alfa/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Antioxidants); 0 (Biological Factors); 0 (Interleukin-1beta); 0 (Peptides, Cyclic); 0 (Tumor Necrosis Factor-alpha); 0 (barettin); SBV4XY874G (Bromine)
[Em] Mês de entrada:1404
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130725
[St] Status:MEDLINE
[do] DOI:10.3390/md11072655


  8 / 27 MEDLINE  
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[PMID]:23695240
[Au] Autor:Vergara A; Grassi M; Sica F; Pizzo E; D'Alessio G; Mazzarella L; Merlino A
[Ad] Endereço:Department of Chemical Sciences, University of Naples 'Federico II', Via Cintia, I-80126 Napoli, Italy.
[Ti] Título:A novel interdomain interface in crystallins: structural characterization of the ßγ-crystallin from Geodia cydonium at 0.99 Å resolution.
[So] Source:Acta Crystallogr D Biol Crystallogr;69(Pt 6):960-7, 2013 Jun.
[Is] ISSN:1399-0047
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The ßγ-crystallin superfamily includes highly diverse proteins belonging to all of the kingdoms of life. Based on structural topology, these proteins are considered to be evolutionarily related to the long-lived ßγ-crystallins that constitute the vertebrate eye lens. This study reports the crystallographic structure at 0.99 Å resolution of the two-domain ßγ-crystallin (geodin) from the sponge Geodia cydonium. This is the most ancient member of the ßγ-crystallin superfamily in metazoans. The X-ray structure shows that the geodin domains adopt the typical ßγ-crystallin fold with a paired Greek-key motif, thus confirming the hypothesis that the crystallin-type scaffold used in the evolution of bacteria and moulds was recruited very early in metazoans. As a significant new structural feature, the sponge protein possesses a unique interdomain interface made up by pairing between the second motif of the first domain and the first motif of the second domain. The atomic resolution also allowed a detailed analysis of the calcium-binding site of the protein.
[Mh] Termos MeSH primário: Cristalinas/química
Geodia/química
[Mh] Termos MeSH secundário: Motivos de Aminoácidos
Animais
Sítios de Ligação
Cristalinas/genética
Cristalografia por Raios X
Evolução Molecular
Geodia/genética
Geodia/metabolismo
Modelos Moleculares
Dobramento de Proteína
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Crystallins)
[Em] Mês de entrada:1310
[Cu] Atualização por classe:130522
[Lr] Data última revisão:
130522
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130523
[St] Status:MEDLINE
[do] DOI:10.1107/S0907444913003569


  9 / 27 MEDLINE  
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[PMID]:22573093
[Au] Autor:Tarakanov AO; Fuxe KG; Borroto-Escuela DO
[Ad] Endereço:Russian Academy of Sciences, St. Petersburg Institute for Informatics and Automation, Saint Petersburg, Russia. sasha_tar@hotmail.com
[Ti] Título:Integrin triplets of marine sponges in human brain receptor heteromers.
[So] Source:J Mol Neurosci;48(1):154-60, 2012 Sep.
[Is] ISSN:1559-1166
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Based on our theory, we have discovered main triplets of amino acid residues in cell-adhesion receptors of marine sponges, which appear also as homologies in several receptor heteromers of human brain. The obtained results strengthen our hypothesis that these triplets may "guide-and-clasp" receptor-receptor interactions.
[Mh] Termos MeSH primário: Encéfalo/fisiologia
Evolução Molecular
Geodia/genética
Integrinas/genética
Receptores de Superfície Celular/genética
[Mh] Termos MeSH secundário: Animais
Seres Humanos
Dados de Sequência Molecular
Filogenia
Receptor de Colecistocinina B/genética
Receptor Tipo 1 de Fator de Crescimento de Fibroblastos/genética
Receptor de Glutamato Metabotrópico 5
Receptor 5-HT1A de Serotonina/genética
Receptor 5-HT1B de Serotonina/genética
Receptor 5-HT1D de Serotonina/genética
Receptores Adrenérgicos alfa 2/genética
Receptores de GABA-B/genética
Receptores de Glutamato Metabotrópico/genética
Homologia de Sequência de Aminoácidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Integrins); 0 (Receptor, Cholecystokinin B); 0 (Receptor, Metabotropic Glutamate 5); 0 (Receptor, Serotonin, 5-HT1B); 0 (Receptor, Serotonin, 5-HT1D); 0 (Receptors, Adrenergic, alpha-2); 0 (Receptors, Cell Surface); 0 (Receptors, GABA-B); 0 (Receptors, Metabotropic Glutamate); 112692-38-3 (Receptor, Serotonin, 5-HT1A); EC 2.7.10.1 (FGFR1 protein, human); EC 2.7.10.1 (Receptor, Fibroblast Growth Factor, Type 1)
[Em] Mês de entrada:1301
[Cu] Atualização por classe:171108
[Lr] Data última revisão:
171108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120511
[St] Status:MEDLINE
[do] DOI:10.1007/s12031-012-9793-6


  10 / 27 MEDLINE  
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[PMID]:22439644
[Au] Autor:Liu WK; Ling YH; Cheung FW; Che CT
[Ad] Endereço:School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, People's Republic of China. ken-liu@cuhk.edu.hk
[Ti] Título:Stellettin A induces endoplasmic reticulum stress in murine B16 melanoma cells.
[So] Source:J Nat Prod;75(4):586-90, 2012 Apr 27.
[Is] ISSN:1520-6025
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Isomalabaricanes are a small class of rearranged triterpenoids obtained from marine sponges. Most of these are cytotoxic to tumor cells, but the underlying mechanism is not clear. In this study, it was demonstrated that stellettin A (1), obtained from Geodia japonica, inhibited the growth of B16F10 murine melanoma cells by the induction of endoplasmic reticulum stress and accumulation of unfolded proteins. Immunoblotting analysis revealed abnormal glycosylation patterns of two melanoma marker proteins, tyrosinase and tyrosinase-related protein 1, and the retention of these proteins in the endoplasmic reticulum. Compound 1 induced the upregulation of the unfolded protein chaperone, glucose-regulated protein 78, in a dose-dependent manner. Increase of autophagosome-associated protein light chain 3 (LC3) in a membrane-bound form (LC3II) and its immunofluorescence co-localization with tyrosinase suggest the possible removal of deglycosylated and unfolded proteins by autophagy of the cells. There was no change in either the expression of the apoptosis marker protein Bcl-2 or the appearance of apoptotic nuclei in 1-treated cells. Taken together, 1 is an endoplasmic reticulum stressor that inhibits the growth of B16 melanoma cells by induction of abnormal protein glycosylation and autophagy.
[Mh] Termos MeSH primário: Antineoplásicos/isolamento & purificação
Antineoplásicos/farmacologia
Estresse do Retículo Endoplasmático/efeitos dos fármacos
Geodia/química
Triterpenos/isolamento & purificação
Triterpenos/farmacologia
[Mh] Termos MeSH secundário: Animais
Antineoplásicos/química
Apoptose/efeitos dos fármacos
Autofagia/efeitos dos fármacos
Relação Dose-Resposta a Droga
Ensaios de Seleção de Medicamentos Antitumorais
Glicosilação/efeitos dos fármacos
Seres Humanos
Biologia Marinha
Melanoma Experimental/metabolismo
Camundongos
Estrutura Molecular
Triterpenos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Triterpenes); 160796-24-7 (stellettin A)
[Em] Mês de entrada:1207
[Cu] Atualização por classe:120427
[Lr] Data última revisão:
120427
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120324
[St] Status:MEDLINE
[do] DOI:10.1021/np2008158



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