Base de dados : MEDLINE
Pesquisa : B01.268.250 [Categoria DeCS]
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  1 / 98 MEDLINE  
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[PMID]:28545140
[Au] Autor:Klein C; Terrao M; Clayton C
[Ad] Endereço:Centre for Molecular Biology of Heidelberg University, DKFZ-ZMBH Alliance, Heidelberg, Germany.
[Ti] Título:The role of the zinc finger protein ZC3H32 in bloodstream-form Trypanosoma brucei.
[So] Source:PLoS One;12(5):e0177901, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Kinetoplastids rely heavily on post-transcriptional mechanisms for control of gene expression, with regulation of mRNA processing, translation and degradation by RNA-binding proteins. ZC3H32 is a cytosolic mRNA-binding protein with three non-canonical CCCH zinc finger domains. It is much more abundant in bloodstream-form Trypanosoma brucei than in procyclic forms. Tethering of ZC3H32 to a reporter mRNA suppressed translation and resulted in mRNA degradation, and deletion analysis suggested that this activity was present in both the N- and C-terminal domains, but not the central zinc finger-containing domain. Tandem affinity purification, however, revealed no interaction partners that might account for this activity. RNASeq analyses did not yield any evidence for sequence-specific binding or regulation of specific mRNAs. The presence of ZC3H32 homologues in monogenetic and free-living Euglenids also argues against a role in developmental regulation, although its function may have diverged in evolution. T. brucei ZC3H32 might be implicated in basal mRNA metabolism, with this role perhaps being taken over by another protein in procyclic forms.
[Mh] Termos MeSH primário: Proteínas de Protozoários/química
Proteínas de Protozoários/metabolismo
RNA Mensageiro/metabolismo
Trypanosoma brucei brucei/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Sítios de Ligação
Euglênidos/metabolismo
Regulação da Expressão Gênica
Domínios Proteicos
RNA de Protozoário/metabolismo
Análise de Sequência de RNA
Trypanosoma brucei brucei/metabolismo
Dedos de Zinco
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Protozoan Proteins); 0 (RNA, Messenger); 0 (RNA, Protozoan)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170526
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0177901


  2 / 98 MEDLINE  
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[PMID]:27678215
[Au] Autor:Hrdá S; Hroudová M; Vlcek C; Hampl V
[Ad] Endereço:Department of Parasitology, Faculty of Science, Charles University, Prague, 128 43, Czech Republic.
[Ti] Título:Mitochondrial Genome of Prasinophyte Alga Pyramimonas parkeae.
[So] Source:J Eukaryot Microbiol;64(3):360-369, 2017 May.
[Is] ISSN:1550-7408
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Prasinophytes are a paraphyletic assemblage of nine heterogeneous lineages in the Chlorophyta clade of Archaeplastida. Until now, seven complete mitochondrial genomes have been sequenced from four prasinophyte lineages. Here, we report the mitochondrial genome of Pyramimonas parkeae, the first representative of the prasinophyte clade I. The circular-mapping molecule is 43,294 bp long, AT rich (68.8%), very compact and it comprises two 6,671 bp long inverted repeat regions. The gene content is slightly smaller than the gene-richest prasinophyte mitochondrial genomes. The single identified intron is located in the cytochrome c oxidase subunit 1 gene (cox1). Interestingly, two exons of cox1 are encoded on the same strand of DNA in the reverse order and the mature mRNA is formed by trans-splicing. The phylogenetic analysis using the data set of 6,037 positions assembled from 34 mtDNA-encoded proteins of 48 green algae and plants is not in compliance with the branching order of prasinophyte clades revealed on the basis of 18S rRNA genes and cpDNA-encoded proteins. However, the phylogenetic analyses based on all three genomic elements support the sister position of prasinophyte clades Pyramimonadales and Mamiellales.
[Mh] Termos MeSH primário: Clorófitas/genética
Genoma Mitocondrial/genética
Proteínas Mitocondriais/classificação
Proteínas Mitocondriais/genética
Filogenia
[Mh] Termos MeSH secundário: Sequência de Bases
Clorófitas/enzimologia
DNA de Cloroplastos/genética
DNA Mitocondrial/genética
DNA de Plantas
Complexo IV da Cadeia de Transporte de Elétrons/genética
Euglênidos/genética
Éxons/genética
Heterogeneidade Genética
Íntrons/genética
Anotação de Sequência Molecular
Proteínas de Plantas/classificação
Proteínas de Plantas/genética
Plantas/genética
RNA Mensageiro/genética
RNA Ribossômico 18S/genética
Trans-Splicing
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Chloroplast); 0 (DNA, Mitochondrial); 0 (DNA, Plant); 0 (Mitochondrial Proteins); 0 (Plant Proteins); 0 (RNA, Messenger); 0 (RNA, Ribosomal, 18S); EC 1.9.3.1 (Electron Transport Complex IV)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170828
[Lr] Data última revisão:
170828
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160929
[St] Status:MEDLINE
[do] DOI:10.1111/jeu.12371


  3 / 98 MEDLINE  
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[PMID]:27718409
[Au] Autor:Cavalier-Smith T; Chao EE; Vickerman K
[Ad] Endereço:Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS, UK. Electronic address: tom.cavalier-smith@zoo.ox.ac.uk.
[Ti] Título:New phagotrophic euglenoid species (new genus Decastava; Scytomonas saepesedens; Entosiphon oblongum), Hsp90 introns, and putative euglenoid Hsp90 pre-mRNA insertional editing.
[So] Source:Eur J Protistol;56:147-170, 2016 Oct.
[Is] ISSN:1618-0429
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:We describe three new phagotrophic euglenoid species by light microscopy and 18S rDNA and Hsp90 sequencing: Scytomonas saepesedens; Decastava edaphica; Entosiphon oblongum. We studied Scytomonas and Decastava ultrastructure. Scytomonas saepesedens feeds when sessile with actively beating cilium, and has five pellicular strips with flush joints and Calycimonas-like microtubule-supported cytopharynx. Decastava, sister to Keelungia forming new clade Decastavida on 18S rDNA trees, has 10 broad strips with cusp-like joints, not bifurcate ridges like Ploeotia and Serpenomonas (phylogenetically and cytologically distinct genera), and Serpenomonas-like feeding apparatus (8-9 unreinforced microtubule pairs loop from dorsal jaw support to cytostome). Hsp90 and 18S rDNA trees group Scytomonas with Petalomonas and show Entosiphon as the earliest euglenoid branch. Basal euglenoids have rigid longitudinal strips; derived clade Spirocuta has spiral often slideable strips. Decastava Hsp90 genes have introns. Decastava/Entosiphon Hsp90 frameshifts imply insertional RNA editing. Petalomonas is too heterogeneous in pellicle structure for one genus; we retain Scytomonas (sometimes lumped with it) and segregate four former Petalomonas as new genus Biundula with pellicle cross section showing 2-8 smooth undulations and typified by Biundula (=Petalomonas) sphagnophila comb. n. Our taxon-rich site-heterogeneous rDNA trees confirm that Heteronema is excessively heterogeneous; therefore we establish new genus Teloprocta for Heteronema scaphurum.
[Mh] Termos MeSH primário: Euglênidos/citologia
Euglênidos/genética
Proteínas de Choque Térmico HSP90/genética
Edição de RNA/genética
Precursores de RNA/genética
[Mh] Termos MeSH secundário: DNA Ribossômico/genética
Euglênidos/classificação
Íntrons/genética
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Ribosomal); 0 (HSP90 Heat-Shock Proteins); 0 (RNA Precursors)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161009
[St] Status:MEDLINE


  4 / 98 MEDLINE  
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[PMID]:26923034
[Au] Autor:Milanowski R; Guminska N; Karnkowska A; Ishikawa T; Zakrys B
[Ad] Endereço:Department of Molecular Phylogenetics and Evolution, Institute of Botany, Faculty of Biology, University of Warsaw, Warsaw, Poland. milan@biol.uw.edu.pl.
[Ti] Título:Intermediate introns in nuclear genes of euglenids - are they a distinct type?
[So] Source:BMC Evol Biol;16:49, 2016 Feb 29.
[Is] ISSN:1471-2148
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Nuclear genes of euglenids contain two major types of introns: conventional spliceosomal and nonconventional introns. The latter are characterized by variable non-canonical borders, RNA secondary structure that brings intron ends together, and an unknown mechanism of removal. Some researchers also distinguish intermediate introns, which combine features of both types. They form a stable RNA secondary structure and are classified into two subtypes depending on whether they contain one (intermediate/nonconventional subtype) or both (conventional/intermediate subtype) canonical spliceosomal borders. However, it has been also postulated that most introns classified as intermediate could simply be special cases of conventional or nonconventional introns. RESULTS: Sequences of tubB, hsp90 and gapC genes from six strains of Euglena agilis were obtained. They contain four, six, and two or three introns, respectively (the third intron in the gapC gene is unique for just one strain). Conventional introns were present at three positions: two in the tubB gene (at one position conventional/intermediate introns were also found) and one in the gapC gene. Nonconventional introns are present at ten positions: two in the tubB gene (at one position intermediate/nonconventional introns were also found), six in hsp90 (at four positions intermediate/nonconventional introns were also found), and two in the gapC gene. CONCLUSIONS: Sequence and RNA secondary structure analyses of nonconventional introns confirmed that their most strongly conserved elements are base pairing nucleotides at positions +4, +5 and +6/ -8, -7 and -6 (in most introns CAG/CTG nucleotides were observed). It was also confirmed that the presence of the 5' GT/C end in intermediate/nonconventional introns is not the result of kinship with conventional introns, but is due to evolutionary pressure to preserve the purine at the 5' end. However, an example of a nonconventional intron with GC-AG ends was shown, suggesting the possibility of intron type conversion between nonconventional and conventional. Furthermore, an analysis of conventional introns revealed that the ability to form a stable RNA secondary structure by some introns is probably not a result of their relationship with nonconventional introns. It was also shown that acquisition of new nonconventional introns is an ongoing process and can be observed at the level of a single species. In the recently acquired intron in the gapC gene an extended direct repeats at the intron-exon junctions are present, suggesting that double-strand break repair process could be the source of new nonconventional introns.
[Mh] Termos MeSH primário: Euglênidos/genética
Genes de Protozoários
Íntrons
[Mh] Termos MeSH secundário: Pareamento de Bases
Sequência de Bases
Éxons
Proteínas de Choque Térmico HSP90/química
Proteínas de Choque Térmico HSP90/genética
Conformação de Ácido Nucleico
Nucleotídeos/genética
Análise de Sequência de DNA
Spliceossomos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (HSP90 Heat-Shock Proteins); 0 (Nucleotides)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:160303
[Lr] Data última revisão:
160303
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160301
[St] Status:MEDLINE
[do] DOI:10.1186/s12862-016-0620-5


  5 / 98 MEDLINE  
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Texto completo SciELO Brasil
[PMID]:26909632
[Au] Autor:Pastich EA; Gavazza S; Casé MC; Florencio L; Kato MT
[Ad] Endereço:Center of Technology and Geoscience, Universidade Federal de Pernambuco, Recife, PE, Brazil.
[Ti] Título:Structure and dynamics of the phytoplankton community within a maturation pond in a semiarid region.
[So] Source:Braz J Biol;76(1):144-53, 2016 Feb.
[Is] ISSN:1678-4375
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:In northeastern Brazil, stabilization ponds are very suitable for wastewater treatment because of the relative great land availability and environmental conditions (e.g., high temperature) favorable for microorganism optimal development. However, blooms of potentially toxic cyanobacteria may affect the use of these treatment ponds due to resulting effluent poor quality. The objective of this study was to evaluate the dynamics of phytoplankton communities and the occurrence of cyanobacteria in a maturation pond located immediately after a series of two ponds. Temperature, dissolved oxygen, pH, BOD, N, and P were measured during a period of four months when samples were collected from the surface and the bottom of 7 sampling points distributed inside the pond. The phytoplankton of collected samples was also identified and classified using a conventional optical microscopy. Analysis of variance and Tukey test were used to evaluate the results. The three phytoplankton divisions found (Cyanophyta, Chlorophyta, and Euglenophyta) did not change considerably through surface and bottom. However, they changed greatly over the sampled months; great dominance of Cyanophyta was found at April and October, while Chlorophyta dominated the lagoon in September. Low superficial organic loads (between 78 and 109 kg BOD.ha-1.d-1) and N:P ≤ 10 were the determinant factors that favored the predominance of Cyanophyta. The presence of two potentially toxic species of Cyanophyta, Oscillatoria sp. and Microcystis aeruginosa, indicates that caution is required when considering the final destination of treated effluent and suggests a need to assess the risks and benefits associated with the use of the treatment technology.
[Mh] Termos MeSH primário: Biodiversidade
Eutrofização
Fitoplâncton/fisiologia
Tanques
[Mh] Termos MeSH secundário: Líquido Amniótico
Brasil
Clorófitas/fisiologia
Cianobactérias/fisiologia
Euglênidos/fisiologia
Dinâmica Populacional
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1611
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160225
[St] Status:MEDLINE


  6 / 98 MEDLINE  
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[PMID]:26566594
[Au] Autor:Markunas CM; Triemer RE
[Ad] Endereço:Department of Plant Biology, Michigan State University, 612 Wilson Road, 166 Plant Biology Labs, East Lansing, Michigan, 48824.
[Ti] Título:Evolutionary History of the Enzymes Involved in the Calvin-Benson Cycle in Euglenids.
[So] Source:J Eukaryot Microbiol;63(3):326-39, 2016 May.
[Is] ISSN:1550-7408
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Euglenids are an ancient lineage that may have existed as early as 2 billion years ago. A mere 65 years ago, Melvin Calvin and Andrew A. Benson performed experiments on Euglena gracilis and elucidated the series of reactions by which carbon was fixed and reduced during photosynthesis. However, the evolutionary history of this pathway (Calvin-Benson cycle) in euglenids was more complex than Calvin and Benson could have imagined. The chloroplast present today in euglenophytes arose from a secondary endosymbiosis between a phagotrophic euglenid and a prasinophyte green alga. A long period of evolutionary time existed before this secondary endosymbiotic event took place, which allowed for other endosymbiotic events or gene transfers to occur prior to the establishment of the green chloroplast. This research revealed the evolutionary history of the major enzymes of the Calvin-Benson cycle throughout the euglenid lineage and showed that the majority of genes for Calvin-Benson cycle enzymes shared an ancestry with red algae and/or chromophytes suggesting they may have been transferred to the nucleus prior to the acquisition of the green chloroplast.
[Mh] Termos MeSH primário: Evolução Biológica
Euglênidos/enzimologia
Euglênidos/genética
Fotossíntese/fisiologia
[Mh] Termos MeSH secundário: Aldose-Cetose Isomerases/classificação
Aldose-Cetose Isomerases/genética
Aldose-Cetose Isomerases/metabolismo
Teorema de Bayes
Clorófitas/enzimologia
Clorófitas/genética
Clorófitas/fisiologia
Cloroplastos/genética
Enzimas/classificação
Enzimas/genética
Enzimas/metabolismo
Euglênidos/metabolismo
Frutose-Bifosfatase/classificação
Frutose-Bifosfatase/genética
Frutose-Bifosfatase/metabolismo
Transferência Genética Horizontal
Gliceraldeído-3-Fosfato Desidrogenases/classificação
Gliceraldeído-3-Fosfato Desidrogenases/genética
Gliceraldeído-3-Fosfato Desidrogenases/metabolismo
Monoéster Fosfórico Hidrolases/classificação
Monoéster Fosfórico Hidrolases/genética
Monoéster Fosfórico Hidrolases/metabolismo
Fotossíntese/genética
Filogenia
Rodófitas/enzimologia
Simbiose
Triose-Fosfato Isomerase/classificação
Triose-Fosfato Isomerase/genética
Triose-Fosfato Isomerase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Enzymes); EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases); EC 3.1.3.11 (Fructose-Bisphosphatase); EC 3.1.3.2 (Phosphoric Monoester Hydrolases); EC 3.1.3.37 (sedoheptulose-bisphosphatase); EC 5.3.1.- (Aldose-Ketose Isomerases); EC 5.3.1.1 (Triose-Phosphate Isomerase); EC 5.3.1.6 (ribosephosphate isomerase)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170630
[Lr] Data última revisão:
170630
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151115
[St] Status:MEDLINE
[do] DOI:10.1111/jeu.12282


  7 / 98 MEDLINE  
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[PMID]:25976746
[Au] Autor:Bennett MS; Triemer RE
[Ad] Endereço:Department of Plant Biology, Michigan State University, 612 Wilson Rd, Room# 166, Plant Biology Labs, East Lansing, Michigan, 48824, USA.
[Ti] Título:Chloroplast Genome Evolution in the Euglenaceae.
[So] Source:J Eukaryot Microbiol;62(6):773-85, 2015 Nov-Dec.
[Is] ISSN:1550-7408
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Over the last few years multiple studies have been published outlining chloroplast genomes that represent many of the photosynthetic euglenid genera. However, these genomes were scattered throughout the euglenophyceaean phylogenetic tree, and focused on comparisons with Euglena gracilis. Here, we present a study exclusively on taxa within the Euglenaceae. Six new chloroplast genomes were characterized, those of Cryptoglena skujai, E. gracilis var. bacillaris, Euglena viridis, Euglenaria anabaena, Monomorphina parapyrum, and Trachelomonas volvocina, and added to six previously published chloroplast genomes to determine if trends existed within the family. With this study: at least one genome has now been characterized for each genus, the genomes of different strains from two taxa were characterized to explore intraspecific variability, and a second taxon has been characterized for the genus Monomorphina to examine intrageneric variability. Overall results showed a large amount of variability among the genomes, though a few trends could be identified both within Euglenaceae and within Euglenophyta. In addition, the intraspecific analysis indicated that the similarity of a genome sequence between strains was taxon dependent, and the intrageneric analysis indicated that the majority of the evolutionary changes within the Euglenaceae occurred intergenerically.
[Mh] Termos MeSH primário: DNA de Cloroplastos/genética
Euglênidos/genética
Evolução Molecular
Genoma de Cloroplastos
[Mh] Termos MeSH secundário: Sequência de Bases
Mapeamento Cromossômico
Sequência Conservada
DNA de Cloroplastos/química
Euglena gracilis/genética
Euglênidos/classificação
Anotação de Sequência Molecular
Fases de Leitura Aberta
Filogenia
Análise de Sequência de DNA
Homologia de Sequência
Sintenia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (DNA, Chloroplast)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:151023
[Lr] Data última revisão:
151023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150516
[St] Status:MEDLINE
[do] DOI:10.1111/jeu.12235


  8 / 98 MEDLINE  
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[PMID]:25779973
[Au] Autor:Kisielewska G; Kolicka M; Zawierucha K
[Ad] Endereço:Department of Animal Taxonomy and Ecology, Faculty of Biology, Adam Mickiewicz University in Poznan, Umultowska 89, 61-614 Poznan, Poland.
[Ti] Título:Prey or parasite? The first observations of live Euglenida in the intestine of Gastrotricha.
[So] Source:Eur J Protistol;51(2):138-41, 2015 Apr.
[Is] ISSN:1618-0429
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Euglenida are an important element in almost all aquatic ecosystems. They are known to parasitize animals such as copepods and flatworms, but have never been found in any other microscopic group. Gastrotrichs, a phylum of small microinvertebrates, are a constant and important element of marine and freshwater ecosystems. During our observation, 72 live gastrotrich specimens were collected from Europe (Poland, Germany) and South America (Brazil) containing active Euglenida of the genus Heteronema. Euglenida were found in the intestine of 10.4%, 52.6% and 1% of gastrotrichs, respectively. Taking into consideration the existing parasitism in euglenoids, it may be hypothesized that they either constitute fortuitous feed in the gastrotrich intestine or they are parasites or commensals. These observations suggest a new, so far unknown type of interaction between Gastrotricha and protists.
[Mh] Termos MeSH primário: Euglênidos/fisiologia
Invertebrados/parasitologia
[Mh] Termos MeSH secundário: Animais
Euglênidos/citologia
Intestinos/parasitologia
Invertebrados/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1512
[Cu] Atualização por classe:150526
[Lr] Data última revisão:
150526
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150318
[St] Status:MEDLINE


  9 / 98 MEDLINE  
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[PMID]:25377266
[Au] Autor:Karnkowska A; Bennett MS; Watza D; Kim JI; Zakrys B; Triemer RE
[Ad] Endereço:Department of Plant Systematics and Geography, Faculty of Biology, University of Warsaw, Warsaw, Poland; Department of Parasitology, Faculty of Science, Charles University in Prague, Prague, Czech Republic.
[Ti] Título:Phylogenetic Relationships and Morphological Character Evolution of Photosynthetic Euglenids (Excavata) Inferred from Taxon-rich Analyses of Five Genes.
[So] Source:J Eukaryot Microbiol;62(3):362-73, 2015 May-Jun.
[Is] ISSN:1550-7408
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Photosynthetic euglenids acquired chloroplasts by secondary endosymbiosis, which resulted in changes to their mode of nutrition and affected the evolution of their morphological characters. Mapping morphological characters onto a reliable molecular tree could elucidate major trends of those changes. We analyzed nucleotide sequence data from regions of three nuclear-encoded genes (nSSU, nLSU, hsp90), one chloroplast-encoded gene (cpSSU) and one nuclear-encoded chloroplast gene (psbO) to estimate phylogenetic relationships among 59 photosynthetic euglenid species. Our results were consistent with previous works; most genera were monophyletic, except for the polyphyletic genus Euglena, and the paraphyletic genus Phacus. We also analyzed character evolution in photosynthetic euglenids using our phylogenetic tree and eight morphological traits commonly used for generic and species diagnoses, including: characters corresponding to well-defined clades, apomorphies like presence of lorica and mucilaginous stalks, and homoplastic characters like rigid cells and presence of large paramylon grains. This research indicated that pyrenoids were lost twice during the evolution of phototrophic euglenids, and that mucocysts, which only occur in the genus Euglena, evolved independently at least twice. In contrast, the evolution of cell shape and chloroplast morphology was difficult to elucidate, and could not be unambiguously reconstructed in our analyses.
[Mh] Termos MeSH primário: Euglênidos/classificação
Euglênidos/genética
Evolução Molecular
Genes de Protozoários
Filogenia
[Mh] Termos MeSH secundário: Biologia Computacional
Euglênidos/citologia
Proteínas de Choque Térmico HSP90/genética
Complexo de Proteína do Fotossistema II/genética
RNA Ribossômico/genética
RNA Ribossômico 18S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (HSP90 Heat-Shock Proteins); 0 (Photosystem II Protein Complex); 0 (RNA, Ribosomal); 0 (RNA, Ribosomal, 18S); 0 (RNA, ribosomal, 26S)
[Em] Mês de entrada:1601
[Cu] Atualização por classe:150428
[Lr] Data última revisão:
150428
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141108
[St] Status:MEDLINE
[do] DOI:10.1111/jeu.12192


  10 / 98 MEDLINE  
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[PMID]:24956802
[Au] Autor:Kamika I; Momba MN
[Ti] Título:Effect of vanadium toxicity at its different oxidation states on selected bacterial and protozoan isolates in wastewater systems.
[So] Source:Environ Technol;35(13-16):2075-85, 2014 Aug.
[Is] ISSN:0959-3330
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This study assesses and compares vanadium toxicity in its different oxidation states towards bacterial isolates (Pseudomonas putida and Bacillus licheniformis) and protozoan isolates (Peranema sp. and Trachelophyllum sp.). The isolates were exposed to various concentrations of V in mixed liquors and their tolerance to V was assessed at 30 degrees C at a pH of 4. The results revealed that the increase in V oxidation state increased its toxicity to bacterial isolates, whereas its toxicity decreased for protozoan isolates. Among the bacterial isolates, P putida was found to be more tolerant to V3+(24h-median lethal concentration (LC50): 390mg/l), V4+(24h-LC50: 230-250mg/l) and V5+(24h-LC50: 180-200mg/l), whereas for the protozoan isolates, Peranema sp. appeared to be more tolerant to V3+(24 h-LC50: 110-120 mg/l), V4+(24 h-LC50: 160-170 mg/l) and V5+(24 h-LC50: 160-200 mg/l). A comparison of both groups of organisms revealed Trachelophyllum sp. as the most sensitive organism to V at its various oxidation states. The visual and spectrophotometric methods used to assess V reduction revealed that P. putida was the only isolate able to reduce V5+, V4+ and V3+ to V2+ in mixed liquor media. Vanadium (+2) in concentrations of approximately 46.46 mg/l, 29.57 m mg/l and 38.01 mg/l found in the media was treated with V3+, V4+ and V5+, respectively, and inoculated with P. putida. This study revealed that the ability of V reduction, adopted with P putida, can be an effective strategy to remove V from polluted environments. This study also showed that the toxicity of V, in terms of its oxidation states, differs from one species to another and in kingdoms.
[Mh] Termos MeSH primário: Vanádio/toxicidade
Águas Residuais/microbiologia
[Mh] Termos MeSH secundário: Bacillus
Cilióforos
Euglênidos
Oxirredução
Pseudomonas putida
Testes de Toxicidade
Vanádio/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Waste Water); 00J9J9XKDE (Vanadium)
[Em] Mês de entrada:1408
[Cu] Atualização por classe:140624
[Lr] Data última revisão:
140624
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140625
[St] Status:MEDLINE



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