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[PMID]:29324232
[Au] Autor:Aksoy S
[Ad] Endereço:Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT 06510, USA. Electronic address: serap.aksoy@yale.edu.
[Ti] Título:Insect Gut Microbiota: Accessories to the Bite.
[So] Source:Cell Host Microbe;23(1):8-9, 2018 01 10.
[Is] ISSN:1934-6069
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The Leishmania parasite is transmitted via the bite of a sand fly. In this issue of Cell Host & Microbe, Dey et al. (2018) report that sand fly gut microbiota are also transferred to the bite site, promoting neutrophil recruitment and parasite dissemination to distal organs.
[Mh] Termos MeSH primário: Microbioma Gastrointestinal
Psychodidae/parasitologia
[Mh] Termos MeSH secundário: Animais
Seres Humanos
Insetos Vetores/parasitologia
Leishmania
[Pt] Tipo de publicação:JOURNAL ARTICLE; COMMENT
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180223
[Lr] Data última revisão:
180223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180112
[St] Status:MEDLINE


  2 / 6555 MEDLINE  
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Cotrim, Paulo Cesar
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[PMID]:28454918
[Au] Autor:Monteiro LM; Löbenberg R; Ferreira EI; Cotrim PC; Kanashiro E; Rocha M; Chung MC; Bou-Chacra N
[Ad] Endereço:Pharmacy Department, Faculty of Pharmaceutical Sciences, University de São Paulo, São Paulo, Brazil.
[Ti] Título:Targeting Leishmania amazonensis amastigotes through macrophage internalisation of a hydroxymethylnitrofurazone nanostructured polymeric system.
[So] Source:Int J Antimicrob Agents;50(1):88-92, 2017 Jul.
[Is] ISSN:1872-7913
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Dextran-coated poly (n-butyl cyanoacrylate) nanoparticles (PBCA-NPs) were prepared and were evaluated for enhanced delivery of a promising anti-Leishmania drug candidate, hydroxymethylnitrofurazone (NFOH), to phagocytic cells. Currently available chemotherapy for leishmaniasis, such as pentavalent antimonials, presents low safety and efficacy. Furthermore, widespread drug resistance in leishmaniasis is rapidly emerging. To overcome these drawbacks, the use of nanosized delivery systems can reduce systemic drug toxicity and increase the drug concentration in infected macrophages, therefore improving treatment of leishmaniasis. PBCA-NPs containing NFOH (PBCA-NFOH-NPs) were prepared by an anionic emulsion polymerisation method. The z-average and polydispersity index (PDI) were determined by photon correlation spectroscopy, the zeta potential by microelectrophoresis and the entrapment efficiency by HPLC. Cytotoxicity was determined using macrophages from BALB/c mice. Efficacy tests were performed using Leishmania amazonensis promastigotes and amastigotes. The z-average of PBCA-NFOH-NPs was 151.5 ± 61.97 nm, with a PDI of 0.104 ± 0.01, a zeta potential of -10.1 ± 6.49 mV and an entrapment efficiency of 64.47 ± 0.43%. Efficacy in amastigotes revealed IC values of 0.33 µM and 31.2 µM for the nanostructured and free NFOH, respectively (95-fold increase). The cytotoxicity study indicated low toxicity of the PBCA-NFOH-NPs to macrophages. The selectivity index was 370.6, which is 49-fold higher than free NFOH (7.6). Such findings indicated that improved efficacy could be due to NP internalisation following site-specific drug delivery and reactivation of immune protective reactions by the NP components. Thus, PBCA-NFOH-NPs have the potential to significantly improve the treatment of leishmaniasis, with reduced systemic side effects.
[Mh] Termos MeSH primário: Antiprotozoários/metabolismo
Leishmania/efeitos dos fármacos
Macrófagos/parasitologia
Nanopartículas/metabolismo
Nitrofurazona/análogos & derivados
[Mh] Termos MeSH secundário: Animais
Sobrevivência Celular/efeitos dos fármacos
Concentração Inibidora 50
Macrófagos/fisiologia
Camundongos Endogâmicos BALB C
Nanopartículas/toxicidade
Nitrofurazona/metabolismo
Testes de Sensibilidade Parasitária
[Pt] Tipo de publicação:JOURNAL ARTICLE; MULTICENTER STUDY
[Nm] Nome de substância:
0 (Antiprotozoal Agents); 0 (hydroxymethylnitrofurazone); X8XI70B5Z6 (Nitrofurazone)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE


  3 / 6555 MEDLINE  
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[PMID]:29326050
[Au] Autor:Nayak A; Akpunarlieva S; Barrett M; Burchmore R
[Ad] Endereço:Institute of Infection, Immunity and Inflammation and Glasgow Polyomics, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK.
[Ti] Título:A defined medium for Leishmania culture allows definition of essential amino acids.
[So] Source:Exp Parasitol;185:39-52, 2018 Feb.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Axenic culture of Leishmania is generally performed in rich, serum-supplemented media which sustain robust growth over multiple passages. The use of such undefined media, however, obscures proteomic analyses and confounds the study of metabolism. We have established a simple, defined culture medium that supports the sustained growth of promastigotes over multiple passages and which yields parasites that have similar infectivity to macrophages to parasites grown in a conventional semi-defined medium. We have exploited this medium to investigate the amino acid requirements of promastigotes in culture and have found that phenylalanine, tryptophan, arginine, leucine, lysine and valine are essential for viability in culture. Most of the 20 proteogenic amino acids promote growth of Leishmania promastigotes, with the exception of alanine, asparagine, and glycine. This defined medium will be useful for further studies of promastigote substrate requirements, and will facilitate future proteomic and metabolomic analyses.
[Mh] Termos MeSH primário: Aminoácidos Essenciais/metabolismo
Meios de Cultura/química
Leishmania/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Anfotericina B/farmacologia
Animais
Antiprotozoários/farmacologia
Concentração Inibidora 50
Leishmania/efeitos dos fármacos
Leishmania donovani/crescimento & desenvolvimento
Leishmania major/crescimento & desenvolvimento
Leishmania mexicana/crescimento & desenvolvimento
Metotrexato/farmacologia
Pentamidina/farmacologia
Inoculações Seriadas
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids, Essential); 0 (Antiprotozoal Agents); 0 (Culture Media); 673LC5J4LQ (Pentamidine); 7XU7A7DROE (Amphotericin B); YL5FZ2Y5U1 (Methotrexate)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180113
[St] Status:MEDLINE


  4 / 6555 MEDLINE  
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[PMID]:29320544
[Au] Autor:González C; León C; Paz A; López M; Molina G; Toro D; Ortiz M; Cordovez JM; Atencia MC; Aguilera G; Tovar C
[Ad] Endereço:Centro de Investigaciones en Microbiología y Parasitología Tropical, CIMPAT, Departamento de Ciencias Biológicas, Universidad de los Andes, Bogotá, Colombia.
[Ti] Título:Diversity patterns, Leishmania DNA detection, and bloodmeal identification of Phlebotominae sand flies in villages in northern Colombia.
[So] Source:PLoS One;13(1):e0190686, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Leishmaniases are neglected tropical diseases exhibiting complex transmission cycles due to the number of parasite species circulating, sand fly species acting as vectors and infected mammals, including humans, which are defined in the New World as accidental hosts. However, current transmission scenarios are changing, and the disease is no longer exclusively related to forested areas but urban transmission foci occur, involving some species of domestic animals as suspected reservoirs. The aim of this study was to determine the transmission cycles in urban environments by evaluating sand fly diversity, detection of Leishmania DNA, and bloodmeal sources through intra and peridomestic collections. The study was carried out in Colombia, in 13 municipalities of Cordoba department, implementing a methodology that could be further used for the evaluation of vector-borne diseases in villages or towns. Our sampling design included 24 houses randomly selected in each of 15 villages distributed in 13 municipalities, which were sampled in two seasons in 2015 and 2016. Sand flies were collected using CDC light traps placed in intra and peridomestic habitats. In addition to the morphological identification, molecular identification through DNA barcodes was also performed. A total of 19,743 sand flies were collected and 13,848 of them (10,268 females and 3,580 males) were used in molecular procedures. Circulation of two known parasite species-Leishmania infantum and Leishmania panamensis was confirmed. Blood source analyses showed that sand flies fed on humans, particularly in the case of the known L. infantum vector, P. evansi; further analyses are advised to evaluate the reservoirs involved in parasite transmission. Our sampling design allowed us to evaluate potential transmission cycles on a department scale, by defining suspected vector species, parasite species present in different municipalities and feeding habits.
[Mh] Termos MeSH primário: DNA de Protozoário/genética
Comportamento Alimentar
Variação Genética
Insetos Vetores/parasitologia
Leishmania/genética
Psychodidae/parasitologia
[Mh] Termos MeSH secundário: Animais
Colômbia
Psychodidae/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Protozoan)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180210
[Lr] Data última revisão:
180210
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180111
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190686


  5 / 6555 MEDLINE  
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Alves, Carlos Roberto
Côrtes, Luzia Monteiro de Castro
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[PMID]:29175018
[Au] Autor:Alves CR; Souza RS; Charret KDS; Côrtes LMC; Sá-Silva MP; Barral-Veloso L; Oliveira LFG; da Silva FS
[Ad] Endereço:Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Biologia Molecular e Doenças Endêmicas, Avenida Brasil, 4365, Manguinhos, Rio de Janeiro, RJ, CEP: 21040-360, Brazil. Electronic address: calves@ioc.fiocruz.br.
[Ti] Título:Understanding serine proteases implications on Leishmania spp lifecycle.
[So] Source:Exp Parasitol;184:67-81, 2018 Jan.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Serine proteases have significant functions over a broad range of relevant biological processes to the Leishmania spp lifecycle. Data gathered here present an update on the Leishmania spp serine proteases and the status of these enzymes as part of the parasite degradome. The serine protease genes (n = 26 to 28) in Leishmania spp, which encode proteins with a wide range of molecular masses (35 kDa-115 kDa), are described along with their degrees of chromosomal and allelic synteny. Amid 17 putative Leishmania spp serine proteases, only ∼18% were experimentally demonstrated, as: signal peptidases that remove the signal peptide from secretory pre-proteins, maturases of other proteins and with metacaspase-like activity. These enzymes include those of clans SB, SC and SF. Classical inhibitors of serine proteases are used as tools for the characterization and investigation of Leishmania spp. Endogenous serine protease inhibitors, which are ecotin-like, can act modulating host actions. However, crude or synthetic based-natural serine protease inhibitors, such as potato tuber extract, Stichodactyla helianthus protease inhibitor I, fukugetin and epoxy-α-lapachone act on parasitic serine proteases and are promising leishmanicidal agents. The functional interrelationship between serine proteases and other Leishmania spp proteins demonstrate essential functions of these enzymes in parasite physiology and therefore their value as targets for leishmaniasis treatment.
[Mh] Termos MeSH primário: Leishmania/enzimologia
Leishmania/crescimento & desenvolvimento
Estágios do Ciclo de Vida
Serina Proteases/metabolismo
Inibidores de Serino Proteinase/farmacologia
[Mh] Termos MeSH secundário: Animais
Seres Humanos
Leishmania/classificação
Leishmania/genética
Leishmaniose/tratamento farmacológico
Serina Proteases/química
Serina Proteases/classificação
Serina Proteases/genética
Inibidores de Serino Proteinase/química
Inibidores de Serino Proteinase/uso terapêutico
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Serine Proteinase Inhibitors); EC 3.4.- (Serine Proteases)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE


  6 / 6555 MEDLINE  
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[PMID]:28454881
[Au] Autor:Foroutan M; Khademvatan S; Majidiani H; Khalkhali H; Hedayati-Rad F; Khashaveh S; Mohammadzadeh H
[Ad] Endereço:Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran; Student Research Committee, Urmia University of Medical Sciences, Urmia, Iran. Electronic address: m.foroutan@modares.ac.ir.
[Ti] Título:Prevalence of Leishmania species in rodents: A systematic review and meta-analysis in Iran.
[So] Source:Acta Trop;172:164-172, 2017 Aug.
[Is] ISSN:1873-6254
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Leishmaniasis are diverse group of diseases caused by numerous species of genus Leishmania. Herein we have contrived a systematic review and meta-analysis on the prevalence of Leishmania species in rodents of Iran. For this purpose, following the general methodology recommended for systematic reviews and meta-analysis, six English databases (PubMed, Science Direct, Scopus, Ovid, Web of Science and Google Scholar) and four Persian databases (Magiran, SID, Iran Doc and Iran Medex) were explored during January 1995 till June 2015. Papers were selected based on 8 pre-defined inclusion criteria. During the years, a total number of 4485 different rodents were captured; among which 1291 cases were Leishmania positive. The calculated weighted prevalence of Leishmania species in rodents was 23% (95% CI=18-28). Given geographical zones of Iran, the highest and lowest prevalence rate was belonged to North 50% (95% CI=40-61) and West 11% (95% CI=5-17), respectively. Rhombomys opimus (1766), Meriones lybicus (1258) and Tatera indica (488) were the three most abundant captured rodents, while the highest prevalence of Leishmania species was observed in Nesokia indica 48% (95% CI=42-54) and followed by R. opimus 39% (95% CI=30-47). Egger's regression test was performed to detect publication bias, which revealed it may not have a significant influence on overall weighted prevalence estimate (P=0.317). Meta-regression analysis demonstrated that there is no significant relationship between overall prevalence with sample size (P=0.1) and year of publication (P=0.7). The results showed remarkable prevalence of Leishmania species in rodent reservoirs. In future, adopting a suitable strategy for control and combat with rodents is necessary.
[Mh] Termos MeSH primário: Gerbillinae
Leishmania/isolamento & purificação
Leishmaniose Cutânea/veterinária
Doenças dos Roedores/parasitologia
[Mh] Termos MeSH secundário: Animais
Irã (Geográfico)/epidemiologia
Leishmania/classificação
Leishmaniose Cutânea/epidemiologia
Leishmaniose Cutânea/parasitologia
Prevalência
Doenças dos Roedores/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; META-ANALYSIS; REVIEW
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180202
[Lr] Data última revisão:
180202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE


  7 / 6555 MEDLINE  
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[PMID]:29240765
[Au] Autor:Ponte-Sucre A; Gamarro F; Dujardin JC; Barrett MP; López-Vélez R; García-Hernández R; Pountain AW; Mwenechanya R; Papadopoulou B
[Ad] Endereço:Department of Physiological Sciences, Laboratory of Molecular Physiology, Institute of Experimental Medicine, Luis Razetti School of Medicine, Universidad Central de Venezuela, Caracas, Venezuela.
[Ti] Título:Drug resistance and treatment failure in leishmaniasis: A 21st century challenge.
[So] Source:PLoS Negl Trop Dis;11(12):e0006052, 2017 Dec.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Reevaluation of treatment guidelines for Old and New World leishmaniasis is urgently needed on a global basis because treatment failure is an increasing problem. Drug resistance is a fundamental determinant of treatment failure, although other factors also contribute to this phenomenon, including the global HIV/AIDS epidemic with its accompanying impact on the immune system. Pentavalent antimonials have been used successfully worldwide for the treatment of leishmaniasis since the first half of the 20th century, but the last 10 to 20 years have witnessed an increase in clinical resistance, e.g., in North Bihar in India. In this review, we discuss the meaning of "resistance" related to leishmaniasis and discuss its molecular epidemiology, particularly for Leishmania donovani that causes visceral leishmaniasis. We also discuss how resistance can affect drug combination therapies. Molecular mechanisms known to contribute to resistance to antimonials, amphotericin B, and miltefosine are also outlined.
[Mh] Termos MeSH primário: Resistência a Medicamentos
Leishmania/efeitos dos fármacos
Leishmania/patogenicidade
Leishmaniose/tratamento farmacológico
[Mh] Termos MeSH secundário: Anfotericina B/farmacologia
Anfotericina B/uso terapêutico
Antiprotozoários/farmacologia
Antiprotozoários/uso terapêutico
Quimioterapia Combinada
Seres Humanos
Leishmania/genética
Leishmania donovani/efeitos dos fármacos
Leishmania donovani/patogenicidade
Leishmaniose/imunologia
Leishmaniose/parasitologia
Leishmaniose Cutânea/tratamento farmacológico
Leishmaniose Visceral/tratamento farmacológico
Leishmaniose Visceral/parasitologia
Epidemiologia Molecular
Fosforilcolina/análogos & derivados
Fosforilcolina/farmacologia
Fosforilcolina/uso terapêutico
Falha de Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Antiprotozoal Agents); 107-73-3 (Phosphorylcholine); 53EY29W7EC (miltefosine); 7XU7A7DROE (Amphotericin B)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171224
[Lr] Data última revisão:
171224
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171215
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0006052


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[PMID]:29176891
[Au] Autor:de Rezende E; Kawahara R; Peña MS; Palmisano G; Stolf BS
[Ad] Endereço:Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
[Ti] Título:Quantitative proteomic analysis of amastigotes from Leishmania (L.) amazonensis LV79 and PH8 strains reveals molecular traits associated with the virulence phenotype.
[So] Source:PLoS Negl Trop Dis;11(11):e0006090, 2017 Nov.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Leishmaniasis is an antropozoonosis caused by Leishmania parasites that affects around 12 million people in 98 different countries. The disease has different clinical forms, which depend mainly on the parasite genetics and on the immunologic status of the host. The promastigote form of the parasite is transmitted by an infected female phlebotomine sand fly, is internalized by phagocytic cells, mainly macrophages, and converts into amastigotes which replicate inside these cells. Macrophages are important cells of the immune system, capable of efficiently killing intracellular pathogens. However, Leishmania can evade these mechanisms due to expression of virulence factors. Different strains of the same Leishmania species may have different infectivity and metastatic phenotypes in vivo, and we have previously shown that analysis of amastigote proteome can give important information on parasite infectivity. Differential abundance of virulence factors probably accounts for the higher virulence of PH8 strain parasites shown in this work. In order to test this hypothesis, we have quantitatively compared the proteomes of PH8 and LV79 lesion-derived amastigotes using a label-free proteomic approach. METHODOLOGY/PRINCIPAL FINDINGS: In the present work, we have compared lesion development by L. (L.) amazonensis PH8 and LV79 strains in mice, showing that they have different virulence in vivo. Viability and numbers of lesion-derived amastigotes were accordingly significantly different. Proteome profiles can discriminate parasites from the two strains and several proteins were differentially expressed. CONCLUSIONS/SIGNIFICANCE: This work shows that PH8 strain is more virulent in mice, and that lesion-derived parasites from this strain are more viable and more infective in vitro. Amastigote proteome comparison identified GP63 as highly expressed in PH8 strain, and Superoxide Dismutase, Tryparedoxin Peroxidase and Heat Shock Protein 70 as more abundant in LV79 strain. The expression profile of all proteins and of the differential ones precisely classified PH8 and LV79 samples, indicating that the two strains have proteins with different abundances and that proteome profiles correlate with their phenotypes.
[Mh] Termos MeSH primário: Leishmania/classificação
Leishmania/patogenicidade
Leishmaniose/patologia
Proteoma/genética
[Mh] Termos MeSH secundário: Animais
Espectrometria de Massas
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Endogâmicos C57BL
Fenótipo
Proteínas de Protozoários/genética
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Proteome); 0 (Protozoan Proteins)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171219
[Lr] Data última revisão:
171219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0006090


  9 / 6555 MEDLINE  
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[PMID]:29049371
[Au] Autor:Courtenay O; Peters NC; Rogers ME; Bern C
[Ad] Endereço:School of Life Sciences, University of Warwick, Coventry, United Kingdom.
[Ti] Título:Combining epidemiology with basic biology of sand flies, parasites, and hosts to inform leishmaniasis transmission dynamics and control.
[So] Source:PLoS Pathog;13(10):e1006571, 2017 Oct.
[Is] ISSN:1553-7374
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Quantitation of the nonlinear heterogeneities in Leishmania parasites, sand fly vectors, and mammalian host relationships provides insights to better understand leishmanial transmission epidemiology towards improving its control. The parasite manipulates the sand fly via production of promastigote secretory gel (PSG), leading to the "blocked sand fly" phenotype, persistent feeding attempts, and feeding on multiple hosts. PSG is injected into the mammalian host with the parasite and promotes the establishment of infection. Animal models demonstrate that sand flies with the highest parasite loads and percent metacyclic promastigotes transmit more parasites with greater frequency, resulting in higher load infections that are more likely to be both symptomatic and efficient reservoirs. The existence of mammalian and sand fly "super-spreaders" provides a biological basis for the spatial and temporal clustering of clinical leishmanial disease. Sand fly blood-feeding behavior will determine the efficacies of indoor residual spraying, topical insecticides, and bed nets. Interventions need to have sufficient coverage to include transmission hot spots, especially in the absence of field tools to assess infectiousness. Interventions that reduce sand fly densities in the absence of elimination could have negative consequences, for example, by interfering with partial immunity conferred by exposure to sand fly saliva. A deeper understanding of both sand fly and host biology and behavior is essential to ensuring effectiveness of vector interventions.
[Mh] Termos MeSH primário: Leishmania/parasitologia
Leishmaniose/epidemiologia
Leishmaniose/transmissão
Parasitos/parasitologia
Psychodidae/parasitologia
[Mh] Termos MeSH secundário: Animais
Interações Hospedeiro-Patógeno/imunologia
Seres Humanos
Insetos Vetores/parasitologia
Leishmaniose/parasitologia
Parasitos/patogenicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171020
[St] Status:MEDLINE
[do] DOI:10.1371/journal.ppat.1006571


  10 / 6555 MEDLINE  
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[PMID]:29031287
[Au] Autor:Soto LA; Caballero N; Fuentes LR; Muñoz PT; Gómez Echevarría JR; López MP; Bornay Llinares FJ; Stanford JL; Stanford CA; Donoghue HD
[Ad] Endereço:Universidad Miguel Hernández de Elche, Alicante, Spain.
[Ti] Título:Leprosy Associated with Atypical Cutaneous Leishmaniasis in Nicaragua and Honduras.
[So] Source:Am J Trop Med Hyg;97(4):1103-1110, 2017 Oct.
[Is] ISSN:1476-1645
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In Central America, few cases of leprosy have been reported, but the disease may be unrecognized. Diagnosis is based on clinical criteria and histology. Preliminary field work in Nicaragua and Honduras found patients, including many children, with skin lesions clinically suggestive of atypical cutaneous leishmaniasis or indeterminate leprosy. Histology could not distinguish these diseases although acid-fast organisms were visible in a few biopsies. Lesions healed after standard antimicrobial therapy for leprosy. In the present study, patients, family members, and other community members were skin-tested and provided nasal swabs and blood samples. Biopsies were taken from a subgroup of patients with clinical signs of infection. Two laboratories analyzed samples, using local in-house techniques. , spp. and were detected using polymerase chain reactions. DNA was detected in blood samples and nasal swabs, including some cases where leprosy was not clinically suspected. spp. were also detected in blood and nasal swabs. Most biopsies contained DNA and coinfection of spp. with occurred in 33% of cases. DNA was also detected and sequenced from Nicaraguan and Honduran environmental samples. In conclusion, leprosy and leishmaniasis are present in both regions, and leprosy appears to be widespread. The nature of any relationship between these two pathogens and the epidemiology of these infections need to be elucidated.
[Mh] Termos MeSH primário: Leishmania/isolamento & purificação
Leishmaniose Cutânea/diagnóstico
Leishmaniose Cutânea/epidemiologia
Hanseníase/diagnóstico
Hanseníase/epidemiologia
Mycobacterium leprae/isolamento & purificação
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Feminino
Honduras/epidemiologia
Seres Humanos
Masculino
Meia-Idade
Nicarágua/epidemiologia
Reação em Cadeia da Polimerase
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171025
[Lr] Data última revisão:
171025
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:171017
[St] Status:MEDLINE
[do] DOI:10.4269/ajtmh.16-0622



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