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[PMID]:29192424
[Au] Autor:Heras-Mosteiro J; Monge-Maillo B; Pinart M; Lopez Pereira P; Reveiz L; Garcia-Carrasco E; Campuzano Cuadrado P; Royuela A; Mendez Roman I; López-Vélez R
[Ad] Endereço:Department of Preventive Medicine and Public Health & Immunology and Microbiology, Rey Juan Carlos University, Avda. Atenas s/n, Alcorcón, Madrid, Spain, 28922.
[Ti] Título:Interventions for Old World cutaneous leishmaniasis.
[So] Source:Cochrane Database Syst Rev;12:CD005067, 2017 12 01.
[Is] ISSN:1469-493X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Cutaneous leishmaniasis, caused by a parasitic infection, is considered one of the most serious skin diseases in many low- and middle-income countries. Old World cutaneous leishmaniasis (OWCL) is caused by species found in Africa, Asia, the Middle East, the Mediterranean, and India. The most commonly prescribed treatments are antimonials, but other drugs have been used with varying success. As OWCL tends to heal spontaneously, it is necessary to justify the use of systemic and topical treatments. This is an update of a Cochrane Review first published in 2008. OBJECTIVES: To assess the effects of therapeutic interventions for the localised form of Old World cutaneous leishmaniasis. SEARCH METHODS: We updated our searches of the following databases to November 2016: the Cochrane Skin Specialised Register, CENTRAL, MEDLINE, Embase, and LILACS. We also searched five trials registers and checked the reference lists of included studies for further references to relevant randomised controlled trials (RCTs). We wrote to national programme managers, general co-ordinators, directors, clinicians, WHO-EMRO regional officers of endemic countries, pharmaceutical companies, tropical medicine centres, and authors of relevant papers for further information about relevant unpublished and ongoing trials. We undertook a separate search for adverse effects of interventions for Old World cutaneous leishmaniasis in September 2015 using MEDLINE. SELECTION CRITERIA: Randomised controlled trials of either single or combination treatments in immunocompetent people with OWCL confirmed by smear, histology, culture, or polymerase chain reaction. The comparators were either no treatment, placebo/vehicle, and/or another active compound. DATA COLLECTION AND ANALYSIS: Two review authors independently assessed trials for inclusion and risk of bias and extracted data. We only synthesised data when we were able to identify at least two studies investigating similar treatments and reporting data amenable to pooling. We also recorded data about adverse effects from the corresponding search. MAIN RESULTS: We included 89 studies (of which 40 were new to this update) in 10,583 people with OWCL. The studies included were conducted mainly in the Far or Middle East at regional hospitals, local healthcare clinics, and skin disease research centres. Women accounted for 41.5% of the participants (range: 23% to 80%). The overall mean age of participants was 25 years (range 12 to 56). Most studies lasted between two to six months, with the longest lasting two years; average duration was four months. Most studies were at unclear or high risk for most bias domains. A lack of blinding and reporting bias were present in almost 40% of studies. Two trials were at low risk of bias for all domains. Trials reported the causative species poorly.Here we provide results for the two main comparisons identified: itraconazole (200 mg for six to eight weeks) versus placebo; and paromomycin ointment (15% plus 10% urea, twice daily for 14 days) versus vehicle.In the comparison of oral itraconazole versus placebo, at 2.5 months' follow up, 85/125 participants in the itraconazole group achieved complete cure compared to 54/119 in the placebo group (RR 3.70, 95% CI 0.35 to 38.99; 3 studies; 244 participants). In one study, microbiological or histopathological cure of skin lesions only occurred in the itraconazole group after a mean follow-up of 2.5 months (RR 17.00, 95% CI 0.47 to 612.21; 20 participants). However, although the analyses favour oral itraconazole for these outcomes, we cannot be confident in the results due to the very low certainty evidence. More side effects of mild abdominal pain and nausea (RR 2.36, 95% CI 0.74 to 7.47; 3 studies; 204 participants) and mild abnormal liver function (RR 3.08, 95% CI 0.53 to 17.98; 3 studies; 84 participants) occurred in the itraconazole group (as well as reports of headaches and dizziness), compared with the placebo group, but again we rated the certainty of evidence as very low so are unsure of the results.When comparing paromomycin with vehicle, there was no difference in the number of participants who achieved complete cure (RR of 1.00, 95% CI 0.86, 1.17; 383 participants, 2 studies) and microbiological or histopathological cure of skin lesions after a mean follow-up of 2.5 months (RR 1.03, CI 0.88 to 1.20; 383 participants, 2 studies), but the paromomycin group had more skin/local reactions (such as inflammation, vesiculation, pain, redness, or itch) (RR 1.42, 95% CI 0.67 to 3.01; 4 studies; 713 participants). For all of these outcomes, the certainty of evidence was very low, meaning we are unsure about these results.Trial authors did not report the percentage of lesions cured after the end of treatment or speed of healing for either of these key comparisons. AUTHORS' CONCLUSIONS: There was very low-certainty evidence to support the effectiveness of itraconazole and paromomycin ointment for OWCL in terms of cure (i.e. microbiological or histopathological cure and percentage of participants completely cured). Both of these interventions incited more adverse effects, which were mild in nature, than their comparisons, but we could draw no conclusions regarding safety due to the very low certainty of the evidence for this outcome.We downgraded the key outcomes in these two comparisons due to high risk of bias, inconsistency between the results, and imprecision. There is a need for large, well-designed international studies that evaluate long-term effects of current therapies and enable a reliable conclusion about treatments. Future trials should specify the species of leishmaniasis; trials on types caused by Leishmania infantum, L aethiopica, andL donovani are lacking. Research into the effects of treating women of childbearing age, children, people with comorbid conditions, and those who are immunocompromised would also be helpful.It was difficult to evaluate the overall efficacy of any of the numerous treatments due to the variable treatment regimens examined and because RCTs evaluated different Leishmania species and took place in different geographical areas. Some outcomes we looked for but did not find were degree of functional and aesthetic impairment, change in ability to detect Leishmania, quality of life, and emergence of resistance. There were only limited data on prevention of scarring.
[Mh] Termos MeSH primário: Antiprotozoários/uso terapêutico
Itraconazol/uso terapêutico
Leishmaniose Cutânea/terapia
Paromomicina/uso terapêutico
[Mh] Termos MeSH secundário: Adulto
Animais
Anti-Infecciosos/uso terapêutico
Antiprotozoários/administração & dosagem
Terapias Complementares/métodos
Crioterapia/métodos
Extremo Oriente
Feminino
Temperatura Alta/uso terapêutico
Seres Humanos
Itraconazol/administração & dosagem
Terapia a Laser
Leishmania major
Leishmania tropica
Masculino
Meia-Idade
Oriente Médio
Bases para Pomadas/administração & dosagem
Paromomicina/administração & dosagem
Fotoquimioterapia
Ensaios Clínicos Controlados Aleatórios como Assunto
[Pt] Tipo de publicação:JOURNAL ARTICLE; META-ANALYSIS; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Antiprotozoal Agents); 0 (Ointment Bases); 304NUG5GF4 (Itraconazole); 61JJC8N5ZK (Paromomycin)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180123
[Lr] Data última revisão:
180123
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171202
[St] Status:MEDLINE
[do] DOI:10.1002/14651858.CD005067.pub5


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[PMID]:29233048
[Au] Autor:Tonelli M; Gabriele E; Piazza F; Basilico N; Parapini S; Tasso B; Loddo R; Sparatore F; Sparatore A
[Ad] Endereço:a Dipartimento di Farmacia , Università di Genova , Genova , Italy.
[Ti] Título:Benzimidazole derivatives endowed with potent antileishmanial activity.
[So] Source:J Enzyme Inhib Med Chem;33(1):210-226, 2018 Dec.
[Is] ISSN:1475-6374
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Two sets of benzimidazole derivatives were synthesised and tested in vitro for activity against promastigotes of Leishmania tropica and L. infantum. Most of the tested compounds resulted active against both Leishmania species, with IC values in the low micromolar/sub-micromolar range. Among the set of 2-(long chain)alkyl benzimidazoles, whose heterocyclic head was quaternised, compound 8 resulted about 100-/200-fold more potent than miltefosine, even if the selectivity index (SI) versus HMEC-1 cells was only moderately improved. In the set of 2-benzyl and 2-phenyl benzimidazoles, bearing a basic side chain in position 1, compound 28 (2-(4-chlorobenzyl)-1-lupinyl-5-trifluoromethylbenzimidazole) was 12-/7-fold more potent than miltefosine, but exhibited a further improved SI. Therefore, compounds 8 and 28 represent interesting hit compounds, susceptible of structural modification to improve their safety profiles.
[Mh] Termos MeSH primário: Antiprotozoários/farmacologia
Benzimidazóis/farmacologia
Leishmania infantum/efeitos dos fármacos
Leishmania tropica/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Antiprotozoários/síntese química
Antiprotozoários/química
Benzimidazóis/síntese química
Benzimidazóis/química
Linhagem Celular
Sobrevivência Celular/efeitos dos fármacos
Cercopithecus aethiops
Relação Dose-Resposta a Droga
Seres Humanos
Estrutura Molecular
Testes de Sensibilidade Parasitária
Relação Estrutura-Atividade
Células Vero
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiprotozoal Agents); 0 (Benzimidazoles)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171221
[Lr] Data última revisão:
171221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.1080/14756366.2017.1410480


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[PMID]:28880944
[Au] Autor:Amro A; Al-Dwibe H; Gashout A; Moskalenko O; Galafin M; Hamarsheh O; Frohme M; Jaeschke A; Schönian G; Kuhls K
[Ad] Endereço:Faculty of Pharmacy, Al-Quds University, Abu-Dies, Jerusalem, Palestine.
[Ti] Título:Spatiotemporal and molecular epidemiology of cutaneous leishmaniasis in Libya.
[So] Source:PLoS Negl Trop Dis;11(9):e0005873, 2017 Sep.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Cutaneous leishmaniasis (CL) is a major public health problem in Libya. In this paper, we describe the eco-epidemiological parameters of CL during the armed conflict period from January 2011 till December 2012. Current spatiotemporal distributions of CL cases were explored and projected to the future using a correlative modelling approach. In addition the present results were compared with our previous data obtained for the time period 1995-2008. METHODOLOGY/PRINCIPAL FINDINGS: We investigated 312 CL patients who presented to the Dermatology Department at the Tripoli Central Hospital and came from 81 endemic areas distributed in 10 districts. The patients presented with typical localized lesions which appeared commonly on the face, arms and legs. Molecular identification of parasites by a PCR-RFLP approach targeting the ITS1 region of the rDNA was successful for 81 patients with two causative species identified: L. major and L. tropica comprised 59 (72.8%) and 22 (27.2%) cases, respectively. Around 77.3% of L. tropica CL and 57.7% of L. major CL caused single lesions. Five CL patients among our data set were seropositive for HIV. L. tropica was found mainly in three districts, Murqub (27.3%), Jabal al Gharbi (27.3%) and Misrata (13.7%) while L. major was found in two districts, in Jabal al Gharbi (61%) and Jafara (20.3%). Seasonal occurrence of CL cases showed that most cases (74.2%) admitted to the hospital between November and March, L. major cases from November till January (69.4%), and L. tropica cases mainly in January and February (41%). Two risk factors were identified for the two species; the presence of previously infected household members, and the presence of rodents and sandflies in patient's neighborhoods. Spatiotemporal projections using correlative distribution models based on current case data and climatic conditions showed that coastal regions have a higher level of risk due to more favourable conditions for the transmitting vectors. CONCLUSION: Future projection of CL until 2060 showed a trend of increasing incidence of CL in the north-western part of Libya, a spread along the coastal region and a possible emergence of new endemics in the north-eastern districts of Libya. These results should be considered for control programs to prevent the emergence of new endemic areas taking also into consideration changes in socio-economical factors such as migration, conflicts, urbanization, land use and access to health care.
[Mh] Termos MeSH primário: Leishmania major/genética
Leishmania tropica/genética
Leishmaniose Cutânea/epidemiologia
Leishmaniose Cutânea/parasitologia
Análise Espaço-Temporal
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Animais
Criança
Pré-Escolar
DNA de Protozoário/genética
Feminino
Seres Humanos
Lactente
Leishmania major/isolamento & purificação
Leishmania tropica/isolamento & purificação
Leishmaniose Cutânea/diagnóstico
Leishmaniose Cutânea/transmissão
Líbia/epidemiologia
Masculino
Meia-Idade
Psychodidae/parasitologia
Roedores/parasitologia
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Protozoan)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170908
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005873


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[PMID]:28719307
[Au] Autor:Jaouadi K; Bettaieb J; Bennour A; Salem S; Rjeibi MR; Chaabane S; Yazidi R; Khabouchi N; Gharbi A; Salah AB
[Ad] Endereço:Laboratory of Medical Epidemiology, Institut Pasteur de Tunis (IPT), Tunis-Belvédère, Tunisia; University Tunis El Manar, Tunisia.
[Ti] Título:First Report on Natural Infection of with in a Classical Focus of in Tunisia.
[So] Source:Am J Trop Med Hyg;97(1):291-294, 2017 Jul.
[Is] ISSN:1476-1645
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In Tunisia, chronic cutaneous leishmaniasis due to is an important health problem. Its spreading has not been fully elucidated. Information on sandfly vectors, as well as their associated species, is of paramount importance since vector dispersion is one of the major factors responsible for pathogen dissemination. Ninety-seven unfed females belonging to the genera and were collected between June and August 2015 using sticky paper traps. Polymerase chain reaction-restriction fragment length polymorphism analysis of the internal transcribed spacer 1and sequencing were used for detection and identification. In total, 650 sandflies were captured and identified (380 males and 270 females). Ninety-seven unfed females were tested for the presence of parasite DNA. Six were found positive for . This novel finding enhances the understanding of the cycle extension of outside its original focus of Tataouine.
[Mh] Termos MeSH primário: DNA de Protozoário/genética
Leishmania major/fisiologia
Leishmania tropica/fisiologia
Phlebotomus/parasitologia
[Mh] Termos MeSH secundário: Animais
DNA Intergênico/genética
Feminino
Interações Hospedeiro-Parasita
Leishmania major/genética
Leishmania tropica/genética
Filogenia
Tunísia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Intergenic); 0 (DNA, Protozoan)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170731
[Lr] Data última revisão:
170731
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170719
[St] Status:MEDLINE
[do] DOI:10.4269/ajtmh.16-0849


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[PMID]:28403153
[Au] Autor:Karakus M; Nasereddin A; Onay H; Karaca E; Özkeklikçi A; Jaffe CL; Kuhls K; Özbilgin A; Ertabaklar H; Demir S; Özbel Y; Töz S
[Ad] Endereço:Ege University, Faculty of Medicine, Department of Parasitology, Izmir, Turkey.
[Ti] Título:Epidemiological analysis of Leishmania tropica strains and giemsa-stained smears from Syrian and Turkish leishmaniasis patients using multilocus microsatellite typing (MLMT).
[So] Source:PLoS Negl Trop Dis;11(4):e0005538, 2017 Apr.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Turkey is located in an important geographical location, in terms of the epidemiology of vector-borne diseases, linking Asia and Europe. Cutaneous leishmaniasis (CL) is one of the endemic diseases in a Turkey and according to the Ministry Health of Turkey, 45% of CL patients originate from Sanliurfa province located in southeastern Turkey. Herein, the epidemiological status of CL, caused by L. tropica, in Turkey was examined using multilocus microsatellite typing (MLMT) of strains obtained from Turkish and Syrian patients. A total of 38 cryopreserved strains and 20 Giemsa-stained smears were included in the present study. MLMT was performed using 12 highly specific microsatellite markers. Delta K (ΔK) calculation and Bayesian statistics were used to determine the population structure. Three main populations (POP A, B and C) were identified and further examination revealed the presence of three subpopulations for POP B and C. Combined analysis was performed using the data of previously typed L. tropica strains and Mediterranean and Sanliurfa populations were identified. This finding suggests that the epidemiological status of L. tropica is more complicated than expected when compared to previous studies. A new population, comprised of Syrian L. tropica samples, was reported for the first time in Turkey, and the data presented here will provide new epidemiological information for further studies.
[Mh] Termos MeSH primário: Leishmania tropica/isolamento & purificação
Leishmaniose Cutânea/epidemiologia
Repetições de Microssatélites
[Mh] Termos MeSH secundário: Corantes Azur
Teorema de Bayes
DNA de Protozoário/isolamento & purificação
Variação Genética
Mapeamento Geográfico
Seres Humanos
Leishmania tropica/classificação
Tipagem de Sequências Multilocus
Filogenia
Síria/epidemiologia
Turquia/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Azure Stains); 0 (DNA, Protozoan)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170628
[Lr] Data última revisão:
170628
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170414
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005538


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[PMID]:28285601
[Au] Autor:Baneth G; Yasur-Landau D; Gilad M; Nachum-Biala Y
[Ad] Endereço:School of Veterinary Medicine, Hebrew University, P.O. Box 12, Rehovot, 76100, Israel. gad.baneth@mail.huji.ac.il.
[Ti] Título:Canine leishmaniosis caused by Leishmania major and Leishmania tropica: comparative findings and serology.
[So] Source:Parasit Vectors;10(1):113, 2017 Mar 13.
[Is] ISSN:1756-3305
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Infection and clinical disease associated with Leishmania major and Leishmania tropica, two common agents of human cutaneous leishmaniosis, have rarely been reported in dogs. This study describes dogs infected with these Leishmania spp. prevalent in the Middle East and North Africa, and compares the serological response of dogs infected with Leishmania infantum, L. major or L. tropica to whole promastigote antigen enzyme-linked immunosorbent assay (ELISA) of each species and to rK39 dipstick. RESULTS: Leishmania major infection in a 5-month-old male dog was associated with alopecic and ulcerative periocular and limb skin lesions which responded to allopurinol treatment. Infection was detected by skin and blood polymerase chain reaction (PCR) and confirmed by DNA sequencing but the dog was seronegative. Leishmania tropica infection was detected in a 3-month-old female dog co-infected with Babesia vogeli and Anaplasma platys and with no skin lesions. PCR and DNA sequencing of the blood and parasite culture were positive for L. tropica. Sera from 11 dogs infected with L. infantum, L. major or L. tropica were reactive with all three Leishmania spp. antigens except for sera from a dog with L. major infection. No significant differences were found between reactivity of dog sera to the antigen of the infecting species, or to the other Leishmania spp. antigens. Sera from dogs infected with L. infantum and L. tropica were positive with the rK39 antigen kit, while dogs with L. major infection were seronegative. CONCLUSIONS: Skin lesions in L. major infected dogs from this study and previous reports (n = 2) were ulcerative and located on the muzzle, feet and foot pads and not associated with generalized lymphadenomegaly and splenomegaly. In previous L. tropica infections, skin lesions were proliferative mucocutaneous in young dogs (n = 2), or associated with widespread dermatitis, lymphadenomegaly and splenomegaly in older dogs with similarity to L. infantum infection (n = 2). This study suggests that ELISA serology with whole promastigote antigen is not distinctive between L. infantum, L. major and L. tropica canine infections and that some L. major infections are not seropositive. PCR with DNA sequencing should be used to discriminate between canine infections with these three species.
[Mh] Termos MeSH primário: Doenças do Cão/parasitologia
Leishmania major/imunologia
Leishmania tropica/imunologia
Leishmaniose Cutânea/veterinária
[Mh] Termos MeSH secundário: África do Norte/epidemiologia
Animais
Coinfecção
Doenças do Cão/diagnóstico
Doenças do Cão/imunologia
Cães
Ensaio de Imunoadsorção Enzimática/veterinária
Feminino
Seres Humanos
Israel/epidemiologia
Leishmania infantum/genética
Leishmania infantum/imunologia
Leishmania major/genética
Leishmania major/isolamento & purificação
Leishmania tropica/genética
Leishmania tropica/isolamento & purificação
Leishmaniose Cutânea/diagnóstico
Leishmaniose Cutânea/epidemiologia
Leishmaniose Cutânea/parasitologia
Masculino
Oriente Médio/epidemiologia
Reação em Cadeia da Polimerase/veterinária
Pele/parasitologia
Pele/patologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170314
[St] Status:MEDLINE
[do] DOI:10.1186/s13071-017-2050-7


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[PMID]:28222637
[Au] Autor:Eksi F; Özgöztasi O; Karsligil T; Saglam M
[Ad] Endereço:1 Department of Medical Microbiology, Gaziantep University, Gaziantep, Turkey.
[Ti] Título:Genotyping Leishmania promastigotes isolated from patients with cutaneous leishmaniasis in south-eastern Turkey.
[So] Source:J Int Med Res;45(1):114-122, 2017 Feb.
[Is] ISSN:1473-2300
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Objective Cutaneous leishmaniasis (CL) is a significant disease in south-eastern Anatolia because it is prevalent among Syrian refugees. We identified the causative Leishmania species in CL patients using molecular methods. Methods Novy-MacNeal-Nicolle medium was inoculated with aspirated fluid from suspected CL lesions and tested for amastigotes with Giemsa staining. PCR amplified the internal transcribed spacer 1 (ITS1) of the Leishmania genome in cultures containing Leishmania promastigotes from 100 patients, which were genotyped with a restriction fragment length polymorphism (RFLP) analysis. A phylogenetic tree was constructed from ITS1 sequences of 95 culture fluid samples from these patients. Results Leishmania amastigotes were detected in 92% of cultures with growth. Leishmania promastigotes were typed as Leishmania tropica with both PCR-RFLP and sequencing. Conclusions Identification of L. tropica as the causative agent of CL in our region allows the clinical course to be predicted, and guides treatment decisions and preventive measures.
[Mh] Termos MeSH primário: DNA de Protozoário/genética
Leishmania tropica/genética
Leishmaniose Cutânea/diagnóstico
Estágios do Ciclo de Vida/genética
Filogenia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Corantes Azur
DNA Intergênico/genética
Feminino
Genótipo
Seres Humanos
Leishmania tropica/classificação
Leishmania tropica/crescimento & desenvolvimento
Leishmania tropica/isolamento & purificação
Leishmaniose Cutânea/epidemiologia
Leishmaniose Cutânea/parasitologia
Leishmaniose Cutânea/patologia
Masculino
Tipagem Molecular
Polimorfismo de Fragmento de Restrição
Refugiados
Análise de Sequência de DNA
Pele/parasitologia
Pele/patologia
Turquia/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Azure Stains); 0 (DNA, Intergenic); 0 (DNA, Protozoan)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170223
[St] Status:MEDLINE
[do] DOI:10.1177/0300060516677155


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[PMID]:28111133
[Au] Autor:Abamor ES; Allahverdiyev AM; Bagirova M; Rafailovich M
[Ad] Endereço:Yildiz Technical University, Bioengineering Department, Esenler, Istanbul, Turkey.
[Ti] Título:Meglumine antimoniate-TiO2@Ag nanoparticle combinations reduce toxicity of the drug while enhancing its antileishmanial effect.
[So] Source:Acta Trop;169:30-42, 2017 May.
[Is] ISSN:1873-6254
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Currently, the treatment of leishmaniasis is increasingly insufficient as current antileishmanial drugs have many disadvantages such as toxic side effects, high cost, and growing drug resistance. In order to overcome these disadvantages, researchers have recently focused on combination therapy by using pentavalent antimonials in conjunction with other antileihmanial compounds. Our previous study found that TiO2@Ag nanoparticles (TiAgNps) demonstrated significant antileishmanial effects. However, a lethal dose of TiAgNps on L. topica promastigotes was found to be toxic for macrophage cells. Moreover, non-toxic concentrations of TiAgNps were ineffective in inhibiting L. topica promastigotes and amastigotes. Thus, we propose the use of TiAgNps in combination with other antileishmanial compounds like meglumine antimoniate (MA) at non-toxic concentrations, which may increase the efficacies of both agents and decrease their toxicities. Therefore, the aim of this study was to determine in vitro and in vivo antileishmanial efficacies of TiAgNps-MA combinations at non-toxic concentrations and develop a new approach for treatment that lowers the toxicities of pentavalent antimonials to minimal levels and enhances their effectiveness. In vitro screening was performed on L. topica promastigote and amastigote-macropage culture by using MTT assay to determine proliferation, perform infection index analysis, and to conduct a Griess reaction for nitric oxide production, while in vivo antileishmanial assays were applied on Balb/c mice with CL models. The results demonstrated that combinations including TiAgNps and MA at non-toxic concentrations were highly efficacious against both promastigotes and amastigotes, while MA application alone did not show any inhibitory effects. It was determined that combination applications decreased the proliferation of L. topica promastigotes 2- to 5-fold in contrast to use of MA alone, and was dependent on concentrations. Moreover, the use of combinations led to inhibition of L. topica amastigotes at rates ranging between 80% and 95%. Additionally, combinations were found to decrease metabolic activities of each form of the parasite at ranges between 7- to 20-fold, causing programmed-cell death and stimulation of macrophages for intensive production of nitric oxide, which is accepted as an important antileishmanial agent (p<0.05). Furthermore, Σ FIC analysis demonstrated that the tested combinations composed little additive, but mostly synergistic effects for inhibition of promastigotes and amastigotes. According to in vivo screening results, the combinations displayed high antileishmanial activities by successfully healing lesions and significantly reducing parasite burdens. Combined, these results show that TiAgNps-MA combinations were much more effective than use of MA alone at non-toxic concentrations and they possess high potential for development of new antileishmanial drugs to fight against leishmaniasis.
[Mh] Termos MeSH primário: Antiprotozoários/efeitos adversos
Antiprotozoários/farmacologia
Leishmaniose/tratamento farmacológico
Leishmaniose/parasitologia
Meglumina/efeitos adversos
Meglumina/farmacologia
Nanopartículas/química
Compostos Organometálicos/efeitos adversos
Compostos Organometálicos/farmacologia
Titânio/química
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Leishmania tropica/efeitos dos fármacos
Leishmania tropica/crescimento & desenvolvimento
Leishmaniose/patologia
Macrófagos/efeitos dos fármacos
Camundongos
Camundongos Endogâmicos BALB C
Nanopartículas/uso terapêutico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiprotozoal Agents); 0 (Organometallic Compounds); 15FIX9V2JP (titanium dioxide); 6HG8UB2MUY (Meglumine); 75G4TW236W (meglumine antimoniate); D1JT611TNE (Titanium)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170124
[St] Status:MEDLINE


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[PMID]:27986151
[Au] Autor:Taylor LA; Gormley R; Kovarik C
[Ad] Endereço:Department of Pathology and Laboratory Medicine, Hospital of the University of Pennsylvania, Philadelphia, Pennsylvania. Electronic address: Laura.Taylor@uphs.upenn.edu.
[Ti] Título:Leishmania tropica: Combined debulking and imiquimod for the treatment of nonresponsive cutaneous leishmaniasis.
[So] Source:J Am Acad Dermatol;76(1):e13-e14, 2017 Jan.
[Is] ISSN:1097-6787
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Aminoquinolinas/uso terapêutico
Antiprotozoários/uso terapêutico
Procedimentos Cirúrgicos de Citorredução
Leishmania tropica
Leishmaniose Cutânea/tratamento farmacológico
Leishmaniose Cutânea/cirurgia
[Mh] Termos MeSH secundário: Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aminoquinolines); 0 (Antiprotozoal Agents); P1QW714R7M (imiquimod)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161218
[St] Status:MEDLINE


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[PMID]:27498421
[Au] Autor:Azmi K; Krayter L; Nasereddin A; Ereqat S; Schnur LF; Al-Jawabreh A; Abdeen Z; Schönian G
[Ad] Endereço:Al-Quds Nutrition and Health Research Institute, Faculty of Medicine, Al-Quds University, Abu-Deis, West Bank, Palestine; Department of Biochemistry and Molecular Biology, Faculty of Medicine, Al-Quds University, East Jerusalem, Abu-Deis, Palestine. Electronic address: kifaya_alkam@yahoo.com.
[Ti] Título:Increased prevalence of human cutaneous leishmaniasis in Israel and the Palestinian Authority caused by the recent emergence of a population of genetically similar strains of Leishmania tropica.
[So] Source:Infect Genet Evol;50:102-109, 2017 Jun.
[Is] ISSN:1567-7257
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Twelve unlinked microsatellite markers were used to determine the microsatellite profiles of 50 newly and 46 previously typed strains of L. tropica from various Israeli and Palestinian foci. Their microsatellite profiles were compared to those of 99 previously typed strains of L. tropica from 15 countries. Israeli and Palestinian strains of L. tropica fell into three different groups, one of which contained 75 of the 96 Israeli and Palestinian strains. This population separated from all the others at the first hierarchical level by Bayesian statistics and formed a distinct monophyletic group on applying genetic distance and allele frequency analyses. The second cluster contained ten Israeli strains from a specific focus north of the Sea of Galilee, which were previously shown to differ from all other strains of L. tropica in their serological, biochemical and molecular biological parameters. This cluster was closely related to clusters comprising strains of L. tropica from Africa. Four Israeli and five Palestinian strains fell into different genetic entities mostly related to strains from Asian foci of CL. Importation during numerous migrations of humans and, perhaps, infected reservoir animals in the past and, now, through modern travel is the most likely explanation for the existence of so many locally encountered genetic variants of L. tropica in the Israeli-Palestinian region. Geographical and ecological variation may play a role in expanding the genetic heterogeneity once given importations had become established in different foci. Currently, one population is expanding in the area comprising almost all of the Palestinian and Israeli strains of L. tropica isolated since 1996 and investigated in this study, which differ clearly from all other strains of whatsoever origin. This population seems to result from the re-emergence of a previously existing genotype owing to environmental changes and human activities.
[Mh] Termos MeSH primário: DNA de Protozoário/genética
Leishmania tropica/genética
Leishmaniose Cutânea/epidemiologia
Filogenia
[Mh] Termos MeSH secundário: Alelos
Animais
Árabes
Teorema de Bayes
Heterogeneidade Genética
Genótipo
Seres Humanos
Israel/epidemiologia
Leishmania tropica/classificação
Leishmania tropica/isolamento & purificação
Leishmaniose Cutânea/etnologia
Leishmaniose Cutânea/parasitologia
Leishmaniose Cutânea/transmissão
Repetições de Microssatélites
Família Multigênica
Filogeografia
Prevalência
Psychodidae/parasitologia
Viagem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Protozoan)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170921
[Lr] Data última revisão:
170921
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160808
[St] Status:MEDLINE



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