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[PMID]:29462204
[Au] Autor:Li H; Li M; Yang X; Gui X; Chen G; Chu J; He X; Wang W; Han F; Li P
[Ad] Endereço:Research Center for Translational Medicine at Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai, China.
[Ti] Título:Microbial diversity and component variation in Xiaguan Tuo Tea during pile fermentation.
[So] Source:PLoS One;13(2):e0190318, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Xiaguan Tuo Tea is largely consumed by the Chinese, but there is little research into the microbial diversity and component changes during the fermentation of this tea. In this study, we first used fluorescence in situ hybridization (FISH), next-generation sequencing (NGS) and chemical analysis methods to determine the microbial abundance and diversity and the chemical composition during fermentation. The FISH results showed that the total number of microorganisms ranges from 2.3×102 to 4.0×108 cells per gram of sample during fermentation and is mainly dominated by fungi. In the early fermentation stages, molds are dominant (0.6×102~2.8×106 cells/g, 0~35 d). However, in the late stages of fermentation, yeasts are dominant (3.6×104~9.6×106 cells/g, 35~56 d). The bacteria have little effect during the fermentation of tea (102~103 cells/g, <1% of fungus values). Of these fungi, A. niger (Aspergillus niger) and B. adeninivorans (Blastobotrys adeninivorans) are identified as the two most common strains, based on Next-generation Sequencing (NGS) analysis. Peak diversity in tea was observed at day 35 of fermentation (Shannon-Weaver index: 1.195857), and lower diversity was observed on days 6 and 56 of fermentation (Shannon-Weaver index 0.860589 and 1.119106, respectively). During the microbial fermentation, compared to the unfermented tea, the tea polyphenol content decreased by 54%, and the caffeine content increased by 59%. Theanine and free amino acid contents were reduced during fermentation by 81.1 and 92.85%, respectively.
[Mh] Termos MeSH primário: Fermentação
Chá/microbiologia
[Mh] Termos MeSH secundário: Bactérias/isolamento & purificação
China
Contagem de Colônia Microbiana
Fungos/isolamento & purificação
Sequenciamento de Nucleotídeos em Larga Escala
Hibridização in Situ Fluorescente
Chá/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Tea)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180221
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190318


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[PMID]:29431327
[Au] Autor:Donerian LG; Vodianova MA; Tarasova ZE
[Ti] Título:[Microscopic soil fungi - bioindicators organisms contaminated soil].
[So] Source:Gig Sanit;95(9):891-4, 2016.
[Is] ISSN:0016-9900
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:In the paper there are considered methodological issues for the evaluation of soil biota in terms of oil pollution. Experimental studies have shown that under the exposure of a various levels of oil pollution meeting certain gradations of the state and optimal alteration in microbocenosis in sod-podzolic soils, there is occurred a transformation of structure of the complex of micromycetes and the accumulation of toxic species, hardly typical for podzolic soils - primarily represantatives of the genus Aspergillus (A.niger and A. versicolor), Paecilomyces (P.variotii Bainer), Trichoderma (T.hamatum), the genus of phytopathogens Fusarium (F.oxysporum), dermatophytes of genus Sporothrix (S. schenckii) and dark-colored melanin containing fungi of Dematiaceae family. Besides that there are presented data on the study of microbiocenosis of the urban soil, the urban soil differed from the zone soil, but shaped in similar landscape and climatic conditions, and therefore having a tendency to a similar response from the side of microorganisms inhabiting the soil. Isolated complex of soil microscopic fungi is described by many authors as a complex, characteristic for soils of megalopolises. This allowed authors of this work to suggest that in urban soils the gain in the occurrence of pathogenic species micromycetes also increases against a background of chronic, continuously renewed inflow of petroleum hydrocarbons from various sources of pollution. Because changes in the species composition of micromycetes occurred in accordance with the increasing load of oil, so far as microscopic soil fungi can be recommended as a bioindicator organisms for oil. In the article there is also provided information about the distinctive features of modern DNA identification method of soil microscopic fungi and accepted in our country methodology of isolation of micromycetes with the use of a nutrient Czapek medium.
[Mh] Termos MeSH primário: Poluição Ambiental
Fungos
Poluição por Petróleo
Microbiologia do Solo/normas
[Mh] Termos MeSH secundário: DNA Fúngico/análise
Poluentes Ambientais/efeitos adversos
Poluentes Ambientais/análise
Poluição Ambiental/efeitos adversos
Poluição Ambiental/análise
Poluição Ambiental/prevenção & controle
Fungos/classificação
Fungos/genética
Fungos/isolamento & purificação
Seres Humanos
Poluição por Petróleo/efeitos adversos
Poluição por Petróleo/análise
Poluição por Petróleo/prevenção & controle
Saúde Pública/métodos
Saúde Pública/normas
Federação Russa/epidemiologia
Saúde da População Urbana/normas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Fungal); 0 (Environmental Pollutants)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180213
[St] Status:MEDLINE


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[PMID]:29309432
[Au] Autor:Unkovic N; Dimkic I; Stupar M; Stankovic S; Vukojevic J; Ljaljevic Grbic M
[Ad] Endereço:Department for Algology, Mycology and Lichenology, Institute of Botany and Botanical Garden "Jevremovac", Faculty of Biology, University of Belgrade, Belgrade, Serbia.
[Ti] Título:Biodegradative potential of fungal isolates from sacral ambient: In vitro study as risk assessment implication for the conservation of wall paintings.
[So] Source:PLoS One;13(1):e0190922, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The principal purpose of the study was to evaluate in vitro the potential ability of fungal isolates obtained from the painted layer of frescoes and surrounding air to induce symptoms of fresco deterioration, associated with their growth and metabolism, so that the risk of such deterioration can be precisely assessed and appropriate conservation treatments formulated. Biodegradative properties of the tested microfungi were qualitatively characterized through the use of a set of special agar plates: CaCO3 glucose agar (calcite dissolution), casein nutrient agar (casein hydrolysis), Czapek-Dox minimal medium (pigment secretion); and Czapek-Dox minimal broth (acid and alkali production). Most of the tested isolates (71.05%) demonstrated at least one of the degradative properties, with Penicillium bilaiae as the most potent, since it tested positive in all four. The remaining isolates (28.95%) showed no deterioration capabilities and were hence considered unlikely to partake in the complex process of fungal deterioration of murals via the tested mechanisms. The obtained results clearly indicate that utilization of fast and simple plate assays can provide insight into the biodegradative potential of deteriogenic fungi and allow for their separation from allochthonous transients, a prerequisite for precise assessment of the amount of risk posed by a thriving mycobiota to mural paintings.
[Mh] Termos MeSH primário: Arte
Fungos/metabolismo
[Mh] Termos MeSH secundário: Biodegradação Ambiental
Carbonato de Cálcio
Técnicas In Vitro
Medição de Risco
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
H0G9379FGK (Calcium Carbonate)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180109
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190922


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[PMID]:29441920
[Ti] Título:Pancreatic lipase and -amylase inhibitory activities of plants used in Traditional Chinese Medicine (TCM).
[So] Source:Pharmazie;71(7):420-424, 2016 Jul 07.
[Is] ISSN:0031-7144
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:To find new, plant based drugs for the treatment of obesity and/or diabetes mellitus type 2 through the inhibition of essential digestive enzymes, in vitro tests were carried out on selected plants or fungi with weight-reducing, blood glucose-reducing or related potential, used in Traditional Chinese Medicine (TCM). Aqueous and methanolic extracts of 32 Chinese herbal medicines were assayed for their in vitro inhibitory activity against pancreatic lipase (PL) and α-amylase (PA). PL activity was measured by using an enzymatic in vitro assay based on the hydrolysis kinetics of an oleate ester of 4-methylumbelliferone. For the determination of α-amylase activity an enzyme assay based on the hydrolytic cleavage of a modified starch derivative was used. Our findings have shown that the methanolic extract of Lycopus lucidus Turcz. var. hirtus Regel (Lamiaceae) was a very effective PL inhibitor (IC50: 88.3±4.1 µg/mL). A high anti-amylase activity showed the methanolic extract of Trichosanthes kirilowii Maxim. (Curcurbitaceae, IC50: 248.8±67.3 µg/mL). This work provides a priority list of interesting plants for further study with respect to the treatment of obesity and associated metabolic diseases.
[Mh] Termos MeSH primário: Lipase/antagonistas & inibidores
Pâncreas/enzimologia
Plantas/química
alfa-Amilases/antagonistas & inibidores
[Mh] Termos MeSH secundário: Fungos/química
Hidrólise
Himecromona/química
Cinética
Lycopus/química
Medicina Tradicional Chinesa
Pâncreas/efeitos dos fármacos
Extratos Vegetais/farmacologia
Trichosanthes/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Extracts); 3T5NG4Q468 (Hymecromone); EC 3.1.1.3 (Lipase); EC 3.2.1.1 (alpha-Amylases)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180215
[St] Status:MEDLINE
[do] DOI:10.1691/ph.2016.6569


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[PMID]:29212440
[Au] Autor:Gdanetz K; Benucci GMN; Vande Pol N; Bonito G
[Ad] Endereço:Department of Plant Biology, Michigan State University, East Lansing, Michigan, 48824, USA.
[Ti] Título:CONSTAX: a tool for improved taxonomic resolution of environmental fungal ITS sequences.
[So] Source:BMC Bioinformatics;18(1):538, 2017 Dec 06.
[Is] ISSN:1471-2105
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: One of the most crucial steps in high-throughput sequence-based microbiome studies is the taxonomic assignment of sequences belonging to operational taxonomic units (OTUs). Without taxonomic classification, functional and biological information of microbial communities cannot be inferred or interpreted. The internal transcribed spacer (ITS) region of the ribosomal DNA is the conventional marker region for fungal community studies. While bioinformatics pipelines that cluster reads into OTUs have received much attention in the literature, less attention has been given to the taxonomic classification of these sequences, upon which biological inference is dependent. RESULTS: Here we compare how three common fungal OTU taxonomic assignment tools (RDP Classifier, UTAX, and SINTAX) handle ITS fungal sequence data. The classification power, defined as the proportion of assigned OTUs at a given taxonomic rank, varied among the classifiers. Classifiers were generally consistent (assignment of the same taxonomy to a given OTU) across datasets and ranks; a small number of OTUs were assigned unique classifications across programs. We developed CONSTAX (CONSensus TAXonomy), a Python tool that compares taxonomic classifications of the three programs and merges them into an improved consensus taxonomy. This tool also produces summary classification outputs that are useful for downstream analyses. CONCLUSIONS: Our results demonstrate that independent taxonomy assignment tools classify unique members of the fungal community, and greater classification power is realized by generating consensus taxonomy of available classifiers with CONSTAX.
[Mh] Termos MeSH primário: DNA Fúngico/genética
DNA Intergênico/genética
Fungos
Sequenciamento de Nucleotídeos em Larga Escala/métodos
Software
[Mh] Termos MeSH secundário: Microbiologia Ambiental
Fungos/classificação
Fungos/genética
Genoma Fúngico/genética
Genômica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Fungal); 0 (DNA, Intergenic)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171208
[St] Status:MEDLINE
[do] DOI:10.1186/s12859-017-1952-x


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[PMID]:27774728
[Au] Autor:Capriotti AL; Cavaliere C; Foglia P; La Barbera G; Samperi R; Ventura S; Laganà A
[Ad] Endereço:Department of Chemistry, University of Rome "La Sapienza", Rome, Italy.
[Ti] Título:Mycoestrogen determination in cow milk: Magnetic solid-phase extraction followed by liquid chromatography and tandem mass spectrometry analysis.
[So] Source:J Sep Sci;39(24):4794-4804, 2016 Dec.
[Is] ISSN:1615-9314
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Recently, magnetic solid-phase extraction has gained interest because it presents various operational advantages over classical solid-phase extraction. Furthermore, magnetic nanoparticles are easy to prepare, and various materials can be used in their synthesis. In the literature, there are only few studies on the determination of mycoestrogens in milk, although their carryover in milk has occurred. In this work, we wanted to develop the first (to the best of our knowledge) magnetic solid-phase extraction protocol for six mycoestrogens from milk, followed by liquid chromatography and tandem mass spectrometry analysis. Magnetic graphitized carbon black was chosen as the adsorbent, as this carbonaceous material, which is very different from the most diffuse graphene and carbon nanotubes, had already shown selectivity towards estrogenic compounds in milk. The graphitized carbon black was decorated with Fe O , which was confirmed by the characterization analyses. A milk deproteinization step was avoided, using only a suitable dilution in phosphate buffer as sample pretreatment. The overall process efficiency ranged between 52 and 102%, whereas the matrix effect considered as signal suppression was below 33% for all the analytes even at the lowest spiking level. The obtained method limits of quantification were below those of other published methods that employ classical solid-phase extraction protocols.
[Mh] Termos MeSH primário: Estrogênios/análise
Contaminação de Alimentos/análise
Leite/química
[Mh] Termos MeSH secundário: Animais
Bovinos
Cromatografia Líquida de Alta Pressão
Cromatografia Líquida
Feminino
Fungos/química
Nanotubos de Carbono
Extração em Fase Sólida
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Estrogens); 0 (Nanotubes, Carbon)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE
[do] DOI:10.1002/jssc.201600879


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[PMID]:29370854
[Au] Autor:Bopage NS; Kamal Bandara Gunaherath GM; Jayawardena KH; Wijeyaratne SC; Abeysekera AM; Somaratne S
[Ad] Endereço:Department of Chemistry, The Open University of Sri Lanka, Nawala, Nugegoda, Sri Lanka.
[Ti] Título:Dual function of active constituents from bark of Ficus racemosa L in wound healing.
[So] Source:BMC Complement Altern Med;18(1):29, 2018 Jan 25.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Different parts including the latex of Ficus racemosa L. has been used as a medicine for wound healing in the Ayurveda and in the indigenous system of medicine in Sri Lanka. This plant has been evaluated for its wound healing potential using animal models. The aim of this study was to obtain an insight into the wound healing process and identify the potential wound healing active substance/s present in F. racemosa L. bark using scratch wound assay (SWA) as the in-vitro assay method. METHOD: Stem bark extracts of F. racemosa were evaluated using scratch wound assay (SWA) on Baby Hamster Kidney (BHK 21) and Madin-Darby Canine Kidney (MDCK) cell lines and Kirby Bauer disc diffusion assay on common bacteria and fungi for cell migration enhancing ability and antimicrobial activity respectively. Dichloromethane and hexanes extracts which showed cell migration enhancement activity on SWA were subjected to bioactivity directed fractionation using column chromatography followed by preparative thin layer chromatography to identify the compounds responsible for the cell migration enhancement activity. RESULTS: Dichloromethane and hexanes extracts showed cell migration enhancement activity on both cell lines, while EtOAc and MeOH extracts showed antibacterial activity against Staphylococcus and Bacillus species and antifungal activity against Saccharomyces spp. and Candida albicans. Lupeol (1) and ß-sitosterol (2) were isolated as the potential wound healing active compounds which exhibited significant cell migration enhancement activity on BHK 21 and MDCK cell lines (> 80%) in par with the positive control, asiaticoside at a concentration of 25 µM. The optimum concentration of each compound required for the maximum wound healing has been determined as 30 µM and 35 µM for 1 and 2 respectively on both cell lines. It is also established that lupeol acetate (3) isolated from the hexanes extract act as a pro-drug by undergoing hydrolysis into lupeol in the vicinity of cells. CONCLUSION: Different chemical constituents present in stem bark of Ficus racemosa L show enhancement of cell migration (which corresponds to the cell proliferation) as well as antimicrobial activity. This dual action of F. racemosa stem bark provides scientific support for its traditional use in wound healing.
[Mh] Termos MeSH primário: Anti-Infecciosos/farmacologia
Ficus/química
Extratos Vegetais/farmacologia
Cicatrização/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Anti-Infecciosos/química
Bactérias/efeitos dos fármacos
Linhagem Celular
Cães
Fungos/efeitos dos fármacos
Células Madin Darby de Rim Canino
Testes de Sensibilidade Microbiana
Casca de Planta/química
Extratos Vegetais/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Plant Extracts)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180127
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-018-2089-9


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[PMID]:29361962
[Au] Autor:Fatima I; Kanwal S; Mahmood T
[Ad] Endereço:Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, 45320, Pakistan.
[Ti] Título:Evaluation of biological potential of selected species of family Poaceae from Bahawalpur, Pakistan.
[So] Source:BMC Complement Altern Med;18(1):27, 2018 Jan 24.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Oxidative stress as well as bacterial and fungal infections are common source of diseases while plants are source of medication for curative or protective purposes. Hence, aim of study was to compare the pharmacological potential of seven grass species in two different solvents i.e. ethanol and acetone. METHODS: Preliminary phytochemical tests were done and antioxidant activities were evaluated using ELISA and their IC50 values and AAI (%) were recorded. ANOVA was used for statistical analyses. DNA damage protection assay was done using p1391Z plasmid DNA and DNA bands were analyzed. Antimicrobial activity was done via disc diffusion method and MIC and Activity Index were determined. Cytotoxic activity was carried out using the brine shrimps' assay and LC50 values were calculated using probit analysis program. RESULTS: Phytochemical studies confirmed the presence of secondary metabolites in most of the plant extracts. Maximum antioxidant potential was revealed in DiAEE, DiAAE (AAI- 54.54% and 43.24%) and DaAEE and DaAAE (AAI- 49.13% and 44.52%). However, PoAEE and PoAAE showed minimum antioxidant potential (AAI- 41.04% and 34.11%). SaSEE, DiAEE and ElIEE showed very little DNA damage protection activity. In antimicrobial assay, DaAEE significantly inhibited the growth of most of the microbial pathogens (nine microbes out of eleven tested microbes) among ethanol extracts while DaAAE and ImCAE showed maximum inhibition (eight microbes out of eleven tested microbes) among acetone plant extracts. However, PoAEE and PoAAE showed least antimicrobial activity. F. oxysporum and A. niger were revealed as the most resistant micro-organisms. ImCEA and ImCAE showed maximum cytotoxic potential (LC50 11.004 ppm and 7.932 ppm) as compared to the other plant extracts. CONCLUSION: Fodder grasses also contains a substantial phenols and flavonoids contents along with other secondary metabolites and, hence, possess a significant medicinal value. Ethanol extracts showed more therapeutic potential as compared to the acetone extracts. This study provides experimental evidence that the selected species contains such valuable natural compounds which can be used as medicinal drugs in future.
[Mh] Termos MeSH primário: Antioxidantes/farmacologia
Extratos Vegetais/farmacologia
Poaceae/química
[Mh] Termos MeSH secundário: Animais
Anti-Infecciosos/química
Anti-Infecciosos/farmacologia
Antioxidantes/química
Artemia/efeitos dos fármacos
Bactérias/efeitos dos fármacos
Dano ao DNA/efeitos dos fármacos
Flavonoides/química
Flavonoides/farmacologia
Fungos/efeitos dos fármacos
Paquistão
Fenóis/química
Fenóis/farmacologia
Extratos Vegetais/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Antioxidants); 0 (Flavonoids); 0 (Phenols); 0 (Plant Extracts)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180125
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-018-2092-1


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[PMID]:29386016
[Au] Autor:Ali S; Khan MR; Irfanullah; Sajid M; Zahra Z
[Ad] Endereço:Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, 45320, Pakistan.
[Ti] Título:Phytochemical investigation and antimicrobial appraisal of Parrotiopsis jacquemontiana (Decne) Rehder.
[So] Source:BMC Complement Altern Med;18(1):43, 2018 Jan 31.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Parrotiopsis jacquemontiana (Decne) Rehder. is locally used for skin infections and in wound healing. In this study we have evaluated methanol extract of its leaves and derived fractions against the clinical multi-drug resistant bacterial strains. METHODS: P. jacquemontiana leaves powder extracted with 95% methanol (PJM) and fractionated in escalating polarity of solvents; n-hexane (PJH), chloroform (PJC), ethyl acetate (PJE), n-butanol (PJB) and the remaining as aqueous fraction (PJA). Clinical as well as environmental 19 bacterial strains and 8 fungal strains were screened for minimum inhibitory concentration (MIC) and minimum bactericidal/fungicidal concentration (MBC/MFC). Preliminary phytochemical investigation for various phytochemical classes was also carried out. RESULTS: PJM contained the coumarins, phenols, flavonoids, tannins, alkaloids, glycosides, saponins, sterols, phlobatannins, steroids, phytosterols, triterpenoids, acids, quinones, proteins, vitamin C, betacyanins, oils and resins while anthraquinones, phytosteroids, carbohydrates and anthocyanins were not detected. Disc diffusion assay (1 mg/disc) indicated the sensitivity of all the MDR strains of bacteria with PJM, PJE and PJB, while no inhibition was recorded with PJA. PJH and PJC inhibited the growth of all the strains of Staphylococcus aureus, Pseudomonas aeruginosa and Coagulase negative staphylococci used in this study. Maximum zone of inhibition (35.5 ± 1.32 mm) was obtained with PJM against Staphylococcus lugdenesis MDR (6197). Comparatively lower MIC (8-64 µg/ml) and MBC (32-256 µg/ml) values were recorded for PJM and PJE. In case of fungal strains only PJM, PJE and PJB markedly inhibited the growth and lower MIC (8-128 µg/ml) and MFC (32-512 µg/ml) values were determined for PJM and PJE. CONCLUSION: The remarkable inhibition of various bacterial and fungal strains at low doses of the extract/fractions suggested the strong antibacterial, antifungal and anti-candidal potential of P. jacquemontiana leaves.
[Mh] Termos MeSH primário: Anti-Infecciosos/farmacologia
Hamamelidaceae/química
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Anti-Infecciosos/química
Anti-Infecciosos/isolamento & purificação
Bactérias/efeitos dos fármacos
Fungos/efeitos dos fármacos
Testes de Sensibilidade Microbiana
Extratos Vegetais/química
Extratos Vegetais/isolamento & purificação
Folhas de Planta/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Plant Extracts)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180202
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-018-2114-z


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[PMID]:28669223
[Au] Autor:Chokpaiboon S; Unagul P; Nithithanasilp S; Komwijit S; Somyong W; Ratiarpakul T; Isaka M; Bunyapaiboonsri T
[Ad] Endereço:a National Center for Genetic Engineering and Biotechnology (BIOTEC) , National Science and Technology Development Agency (NSTDA) , Pathum Thani , Thailand.
[Ti] Título:Salicylaldehyde and dihydroisobenzofuran derivatives from the marine fungus Zopfiella marina.
[So] Source:Nat Prod Res;32(2):149-153, 2018 Jan.
[Is] ISSN:1478-6427
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Two salicylaldehyde derivatives (1 and 2), a hydroxymethylphenol (3), five dihydroisobenzofuran (4-8) derivatives, and a 5-chloro-3-deoxyisoochracinic acid (9), together with a known 3-deoxyisoochracinic acid (10) were isolated from the marine fungus Zopfiella marina BCC 18240 (or NBRC 30420). The structures of these compounds were elucidated by extensive spectroscopic analysis. Compound 1 showed weak antituberculous activity against Mycobacterium tuberculosis H37Ra, and antibacterial activity against Bacillus cereus with MIC values of 25 and 12.5 µg/mL, respectively.
[Mh] Termos MeSH primário: Aldeídos/isolamento & purificação
Antibacterianos/isolamento & purificação
Benzofuranos/isolamento & purificação
Fungos/química
[Mh] Termos MeSH secundário: Aldeídos/farmacologia
Antibacterianos/química
Antibacterianos/farmacologia
Bacillus cereus/efeitos dos fármacos
Biologia Marinha
Testes de Sensibilidade Microbiana
Estrutura Molecular
Mycobacterium tuberculosis/efeitos dos fármacos
Análise Espectral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aldehydes); 0 (Anti-Bacterial Agents); 0 (Benzofurans); 17K64GZH20 (salicylaldehyde)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180228
[Lr] Data última revisão:
180228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170704
[St] Status:MEDLINE
[do] DOI:10.1080/14786419.2017.1342083



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