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[PMID]:28372209
[Au] Autor:Akanbi TO; Barrow CJ
[Ad] Endereço:Centre for Chemistry and Biotechnology, Deakin University, Locked Bag 20000, Geelong, VIC, Australia.
[Ti] Título:Candida antarctica lipase A effectively concentrates DHA from fish and thraustochytrid oils.
[So] Source:Food Chem;229:509-516, 2017 Aug 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The fatty acid selectivity of Candida antarctica lipase A (CAL-A) was applied to produce DHA concentrate by controlling the rate and extent of hydrolysis. Calcium was utilized to achieve a higher degree of hydrolysis. CAL-A was not regioselective but rather fatty acid selective, showing sequential selectivity for saturated, monounsaturated and polyunsaturated fatty acids in the order of increasing double bonds. Based on its strong initial preference for saturates, CAL-A was used to concentrate 82% docosahexaenoic acid (DHA) and 11% omega-6 docosapentaenoic acid (DPA-n6) after partial hydrolysis of algal oil. Thermomyces lanuginosus (TL 100L) lipase was used to partially remove DPA-n6, further concentrating DHA to 89%. CAL-A was immobilized on octadecyl-activated resin without altering its fatty acid selectivity.
[Mh] Termos MeSH primário: Candida/enzimologia
Ácidos Docosa-Hexaenoicos
Peixes/metabolismo
Lipase/metabolismo
Óleos/química
Estramenópilas/química
[Mh] Termos MeSH secundário: Animais
Eurotiales/enzimologia
Proteínas Fúngicas/metabolismo
Hidrólise
Óleos/metabolismo
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungal Proteins); 0 (Oils); 25167-62-8 (Docosahexaenoic Acids); EC 3.1.1.3 (Lipase)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170515
[Lr] Data última revisão:
170515
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170405
[St] Status:MEDLINE


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[PMID]:28194717
[Au] Autor:Sadighi A; Motevalizadeh SF; Hosseini M; Ramazani A; Gorgannezhad L; Nadri H; Deiham B; Ganjali MR; Shafiee A; Faramarzi MA; Khoobi M
[Ad] Endereço:Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, Kingston, RI, 02881, USA.
[Ti] Título:Metal-Chelate Immobilization of Lipase onto Polyethylenimine Coated MCM-41 for Apple Flavor Synthesis.
[So] Source:Appl Biochem Biotechnol;182(4):1371-1389, 2017 Aug.
[Is] ISSN:1559-0291
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:An enzyme immobilized on a mesoporous silica nanoparticle can serve as a multiple catalyst for the synthesis of industrially useful chemicals. In this work, MCM-41 nanoparticles were coated with polyethylenimine (MCM-41@PEI) and further modified by chelation of divalent metal ions (M = Co , Cu , or Pd ) to produce metal-chelated silica nanoparticles (MCM-41@PEI-M). Thermomyces lanuginosa lipase (TLL) was immobilized onto MCM-41, MCM-41@PEI, and MCM-41@PEI-M by physical adsorption. Maximum immobilization yield and efficiency of 75 ± 3.5 and 65 ± 2.7% were obtained for MCM@PEI-Co, respectively. The highest biocatalytic activity at extremely acidic and basic pH (pH = 3 and 10) values were achieved for MCM-PEI-Co and MCM-PEI-Cu, respectively. Optimum enzymatic activity was observed for MCM-41@PEI-Co at 75 °C, while immobilized lipase on the Co-chelated support retained 70% of its initial activity after 14 days of storage at room temperature. Due to its efficient catalytic performance, MCM-41@PEI-Co was selected for the synthesis of ethyl valerate in the presence of valeric acid and ethanol. The enzymatic esterification yield for immobilized lipase onto MCM-41@PEI-Co was 60 and 53%, respectively, after 24 h of incubation in n-hexane and dimethyl sulfoxide media. Graphical Abstract Divalent metal chelated polyethylenimine coated MCM-41 (MCM-41@PEI-M) was used for immobilization of Thermomyces lanuginosa lipase catalyzing green apple flavor preparation.
[Mh] Termos MeSH primário: Cátions Bivalentes/química
Enzimas Imobilizadas/química
Lipase/química
Nanopartículas/química
Ácidos Pentanoicos/síntese química
Polietilenoimina/química
Dióxido de Silício/química
[Mh] Termos MeSH secundário: Adsorção
Técnicas de Química Sintética
Enzimas Imobilizadas/metabolismo
Esterificação
Eurotiales/enzimologia
Concentração de Íons de Hidrogênio
Lipase/metabolismo
Modelos Moleculares
Conformação Proteica
Propriedades de Superfície
Temperatura Ambiente
Valeratos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cations, Divalent); 0 (Enzymes, Immobilized); 0 (MCM-41); 0 (Pentanoic Acids); 0 (Valerates); 0 (ethyl valerate); 7631-86-9 (Silicon Dioxide); 9002-98-6 (Polyethyleneimine); EC 3.1.1.3 (Lipase); GZK92PJM7B (n-pentanoic acid)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170908
[Lr] Data última revisão:
170908
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170215
[St] Status:MEDLINE
[do] DOI:10.1007/s12010-017-2404-9


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[PMID]:27909876
[Au] Autor:Nadar SS; Rathod VK
[Ad] Endereço:Department of Chemical Engineering, Institute of Chemical Technology, Matunga (E), Mumbai, 400019, India.
[Ti] Título:Sonochemical Effect on Activity and Conformation of Commercial Lipases.
[So] Source:Appl Biochem Biotechnol;181(4):1435-1453, 2017 Apr.
[Is] ISSN:1559-0291
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The enzyme under lower-intensity ultrasonic irradiation leads to favourable conformational changes, thereby enhancing its activity. The augmentation of activity of ultrasound-treated enzyme is strongly dependent on ultrasound intensity, duty cycle and exposure time, which was investigated for commercial lipases. Thermomyces lanuginosus (TL) lipase showed a 1.3-fold enhanced activity after irradiating at 22 kHz and 11.38 W cm with 50 % duty cycle for 25-min ultrasonic treatment and 1.5-fold enhanced activity was observed for lipozyme (candida antarctica lipase B (CALB)) lipase, at 22 kHz and 15.48 W cm with 66.67 % duty cycle for 20-min ultrasonic treatment. After sonication, thermodynamic parameters viz. E , ΔH, ΔS and ΔG were evaluated and values were found to be significantly lower for both lipases. In addition, the changes in secondary structure due to sonication were investigated by using Fourier transform infrared (FT-IR), which revealed increase in a certain number of random coiled structure, loss of ß-sheets, ß-turns and α-helix content in TL lipase and CALB lipase. Also, fluorescence spectroscopy exhibited the increased number of tryptophan on surface of both lipases. Moreover, particle size distribution after sonication also helped to improve surface area and enhanced mass transfer, which contributed to improvement in lipase activity.
[Mh] Termos MeSH primário: Lipase/química
Lipase/metabolismo
Ondas Ultrassônicas
[Mh] Termos MeSH secundário: Eurotiales/enzimologia
Cinética
Tamanho da Partícula
Estrutura Secundária de Proteína
Termodinâmica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 3.1.1.3 (Lipase)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170425
[Lr] Data última revisão:
170425
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161203
[St] Status:MEDLINE
[do] DOI:10.1007/s12010-016-2294-2


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[PMID]:27631633
[Au] Autor:Stevenson A; Hamill PG; Medina Á; Kminek G; Rummel JD; Dijksterhuis J; Timson DJ; Magan N; Leong SL; Hallsworth JE
[Ad] Endereço:Institute for Global Food Security, School of Biological Sciences, MBC, Queen's University Belfast, Belfast, BT9 7BL, Northern Ireland.
[Ti] Título:Glycerol enhances fungal germination at the water-activity limit for life.
[So] Source:Environ Microbiol;19(3):947-967, 2017 Mar.
[Is] ISSN:1462-2920
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:For the most-extreme fungal xerophiles, metabolic activity and cell division typically halts between 0.700 and 0.640 water activity (approximately 70.0-64.0% relative humidity). Here, we investigate whether glycerol can enhance xerophile germination under acute water-activity regimes, using an experimental system which represents the biophysical limit of Earth's biosphere. Spores from a variety of species, including Aspergillus penicillioides, Eurotium halophilicum, Xerochrysium xerophilum (formerly Chrysosporium xerophilum) and Xeromyces bisporus, were produced by cultures growing on media supplemented with glycerol (and contained up to 189 mg glycerol g dry spores ). The ability of these spores to germinate, and the kinetics of germination, were then determined on a range of media designed to recreate stresses experienced in microbial habitats or anthropogenic systems (with water-activities from 0.765 to 0.575). For A. penicillioides, Eurotium amstelodami, E. halophilicum, X. xerophilum and X. bisporus, germination occurred at lower water-activities than previously recorded (0.640, 0.685, 0.651, 0.664 and 0.637 respectively). In addition, the kinetics of germination at low water-activities were substantially faster than those reported previously. Extrapolations indicated theoretical water-activity minima below these values; as low as 0.570 for A. penicillioides and X. bisporus. Glycerol is present at high concentrations (up to molar levels) in many types of microbial habitat. We discuss the likely role of glycerol in expanding the water-activity limit for microbial cell function in relation to temporal constraints and location of the microbial cell or habitat. The findings reported here have also critical implications for understanding the extremes of Earth's biosphere; for understanding the potency of disease-causing microorganisms; and in biotechnologies that operate at the limits of microbial function.
[Mh] Termos MeSH primário: Fungos/fisiologia
Glicerol/metabolismo
Esporos Fúngicos/fisiologia
Água/metabolismo
[Mh] Termos MeSH secundário: Aspergillus/metabolismo
Ecossistema
Eurotiales/metabolismo
Fungos/metabolismo
Esporos Fúngicos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
059QF0KO0R (Water); PDC6A3C0OX (Glycerol)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170822
[Lr] Data última revisão:
170822
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160916
[St] Status:MEDLINE
[do] DOI:10.1111/1462-2920.13530


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[PMID]:27562192
[Au] Autor:Stevenson A; Hamill PG; Dijksterhuis J; Hallsworth JE
[Ad] Endereço:Institute for Global Food Security, School of Biological Sciences, MBC, Queen's University Belfast, Belfast, BT9 7BL, UK.
[Ti] Título:Water-, pH- and temperature relations of germination for the extreme xerophiles Xeromyces bisporus (FRR 0025), Aspergillus penicillioides (JH06THJ) and Eurotium halophilicum (FRR 2471).
[So] Source:Microb Biotechnol;10(2):330-340, 2017 Mar.
[Is] ISSN:1751-7915
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Water activity, temperature and pH are determinants for biotic activity of cellular systems, biosphere function and, indeed, for all life processes. This study was carried out at high concentrations of glycerol, which concurrently reduces water activity and acts as a stress protectant, to characterize the biophysical capabilities of the most extremely xerophilic organisms known. These were the fungal xerophiles: Xeromyces bisporus (FRR 0025), Aspergillus penicillioides (JH06THJ) and Eurotium halophilicum (FRR 2471). High-glycerol spores were produced and germination was determined using 38 media in the 0.995-0.637 water activity range, 33 media in the 2.80-9.80 pH range and 10 incubation temperatures, from 2 to 50°C. Water activity was modified by supplementing media with glycerol+sucrose, glycerol+NaCl and glycerol+NaCl+sucrose which are known to be biologically permissive for X. bisporus, A. penicillioides and E. halophilicum respectively. The windows and rates for spore germination were quantified for water activity, pH and temperature; symmetry/asymmetry of the germination profiles were then determined in relation to supra- and sub-optimal conditions; and pH- and temperature optima for extreme xerophilicity were quantified. The windows for spore germination were ~1 to 0.637 water activity, pH 2.80-9.80 and > 10 and < 44°C, depending on strain. Germination profiles in relation to water activity and temperature were asymmetrical because conditions known to entropically disorder cellular macromolecules, i.e. supra-optimal water activity and high temperatures, were severely inhibitory. Implications of these processes were considered in relation to the in-situ ecology of extreme conditions and environments; the study also raises a number of unanswered questions which suggest the need for new lines of experimentation.
[Mh] Termos MeSH primário: Meios de Cultura/química
Eurotiales/crescimento & desenvolvimento
Esporos Fúngicos/crescimento & desenvolvimento
Temperatura Ambiente
Água/química
[Mh] Termos MeSH secundário: Eurotiales/efeitos dos fármacos
Eurotiales/efeitos da radiação
Glicerol/metabolismo
Concentração de Íons de Hidrogênio
Cloreto de Sódio/metabolismo
Esporos Fúngicos/efeitos dos fármacos
Esporos Fúngicos/efeitos da radiação
Sacarose/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Culture Media); 059QF0KO0R (Water); 451W47IQ8X (Sodium Chloride); 57-50-1 (Sucrose); PDC6A3C0OX (Glycerol)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170704
[Lr] Data última revisão:
170704
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160827
[St] Status:MEDLINE
[do] DOI:10.1111/1751-7915.12406


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[PMID]:27424518
[Au] Autor:Sørensen TH; Windahl MS; McBrayer B; Kari J; Olsen JP; Borch K; Westh P
[Ad] Endereço:NSM, Research Unit for Functional Biomaterials, Roskilde University, Universitetsvej 1, Building 28, DK-4000, Roskilde, Denmark.
[Ti] Título:Loop variants of the thermophile Rasamsonia emersonii Cel7A with improved activity against cellulose.
[So] Source:Biotechnol Bioeng;114(1):53-62, 2017 Jan.
[Is] ISSN:1097-0290
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cel7A cellobiohydrolases perform processive hydrolysis on one strand of cellulose, which is threaded through the enzyme's substrate binding tunnel. The tunnel structure results from a groove in the catalytic domain, which is covered by a number of loops. These loops have been identified as potential targets for engineering of this industrially important enzyme family, but only few systematic studies on this have been made. Here we show that two asparagine residues (N194 and N197) positioned in the loop covering the glucopyranose subsite -4 (recently denoted B2 loop) of the thermostable Cel7A from Rasamsonia emersonii had profound effects on both substrate interactions and catalytic efficacy. At room temperature the double mutant N194A/N197A showed strongly reduced substrate affinity with a water-cellulose partitioning coefficient threefold lower than the wild type. Yet, this variant was catalytically efficient with a maximal turnover about twice as high as the wild type. Analogous but smaller changes were found for the single mutants. Analysis of these changes in affinity and kinetics as a function of temperature, led to the conclusion that replacement of N194 and particularly N197 with alanine leads to faster enzyme-substrate dissociation. Conversely, these residues appeared to have little or no effect on the rate of association. We suggest that the controlled adjustment of the enzyme-substrate dissociation prompts faster cellulolytic enzymes. Biotechnol. Bioeng. 2017;114: 53-62. © 2016 Wiley Periodicals, Inc.
[Mh] Termos MeSH primário: Celulose 1,4-beta-Celobiosidase/genética
Celulose 1,4-beta-Celobiosidase/metabolismo
Celulose/metabolismo
Eurotiales/enzimologia
Proteínas Fúngicas/genética
Proteínas Fúngicas/metabolismo
Engenharia de Proteínas/métodos
[Mh] Termos MeSH secundário: Celulose/análise
Celulose 1,4-beta-Celobiosidase/química
Eurotiales/genética
Proteínas Fúngicas/química
Cinética
Modelos Moleculares
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungal Proteins); 9004-34-6 (Cellulose); EC 3.2.1.91 (Cellulose 1,4-beta-Cellobiosidase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160719
[St] Status:MEDLINE
[do] DOI:10.1002/bit.26050


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[PMID]:27271725
[Au] Autor:Yang W; Jiang Z; Liu L; Lin Y; Wang L; Zhou S
[Ad] Endereço:School of Food Science and Technology in Jiangnan University, Wuxi, Jiangsu, 214122, China.
[Ti] Título:The effect of pentosanase on the solubilisation and degradation of arabinoxylan extracted from whole and refined wheat flour.
[So] Source:J Sci Food Agric;97(3):1034-1041, 2017 Feb.
[Is] ISSN:1097-0010
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The quality improvement capability of pentosanase (Pn) for whole-wheat Chinese steamed bread (CSB) is not as efficient as that for refined CSB. However, the underlying mechanism remains to be elucidated. In this work, water-extractable arabinoxylan (WEAX) and water-unextractable solids (WUS) were extracted from whole and refined wheat flour, and then treated with Pn under the conditions similar to CSB-making. Solubilisation and degradation of arabinoxylan (AX) caused by Pn treatment were determined. RESULTS: WEAX from whole flour exhibited higher molecular weight than that from refined flour before and after the treatment with equivalent Pn. Compared with WUS from refined flour, WUS from whole flour had a much lower dissolution degree but the degradation of AX released from the WUS was more efficiently. Moreover, AX released from WUS for refined flour showed a higher Ara/Xyl ratio and the percentage of residual ferulic acid in WUS decreased more significantly. CONCLUSION: The difference in quality improvement degree for Pn in whole-wheat and refined CSB might be mainly explained by its effect on WUS. That is, Pn contributed much more to the solubilisation of WUS from refined flour but provoked degradation predominantly on AX solubilised from WUS isolated from whole flour. © 2016 Society of Chemical Industry.
[Mh] Termos MeSH primário: Pão/análise
Farinha/análise
Qualidade dos Alimentos
Triticum/química
Grãos Integrais/química
Xilanos/metabolismo
Xilosidases/metabolismo
[Mh] Termos MeSH secundário: Fenômenos Químicos
China
Culinária
Ácidos Cumáricos/análise
Eurotiales/enzimologia
Manipulação de Alimentos
Proteínas Fúngicas/metabolismo
Hidrólise
Fenômenos Mecânicos
Peso Molecular
Pentoses/metabolismo
Solubilidade
Vapor
Especificidade por Substrato
Viscosidade
Xilanos/química
Xilanos/isolamento & purificação
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Coumaric Acids); 0 (Fungal Proteins); 0 (Pentoses); 0 (Steam); 0 (Xylans); 9040-27-1 (arabinoxylan); AVM951ZWST (ferulic acid); EC 3.2.1.- (Xylosidases); EC 3.2.1.- (arabinoxylanase)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170613
[Lr] Data última revisão:
170613
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160609
[St] Status:MEDLINE
[do] DOI:10.1002/jsfa.7833


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[PMID]:27605712
[Au] Autor:Mouhajir A; Matray O; Giraud S; Mély L; Marguet C; Sermet-Gaudelus I; Le Gal S; Labbé F; Person C; Troussier F; Ballet JJ; Gargala G; Zouhair R; Bougnoux ME; Bouchara JP; Favennec L
[Ad] Endereço:L'UNAM Université, Université d'Angers, Groupe d'Etude des Interactions Hôte-Pathogène, Angers, France.
[Ti] Título:Long-Term Rasamsonia argillacea Complex Species Colonization Revealed by PCR Amplification of Repetitive DNA Sequences in Cystic Fibrosis Patients.
[So] Source:J Clin Microbiol;54(11):2804-2812, 2016 Nov.
[Is] ISSN:1098-660X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The aim of this work was to document molecular epidemiology of Rasamsonia argillacea species complex isolates from cystic fibrosis (CF) patients. In this work, 116 isolates belonging to this species complex and collected from 26 CF patients and one patient with chronic granulomatous disease were characterized using PCR amplification assays of repetitive DNA sequences and electrophoretic separation of amplicons (rep-PCR). Data revealed a clustering consistent with molecular species identification. A single species was recovered from most patients. Rasamsonia aegroticola was the most common species, followed by R. argillacea sensu stricto and R. piperina, while R. eburnea was not identified. Of 29 genotypes, 7 were shared by distinct patients while 22 were patient specific. In each clinical sample, most isolates exhibited an identical genotype. Genotyping of isolates recovered from sequential samples from the same patient confirmed the capability of R. aegroticola and R. argillacea isolates to chronically colonize the airways. A unique genotype was recovered from two siblings during a 6-month period. In the other cases, a largely dominant genotype was detected. Present results which support the use of rep-PCR for both identification and genotyping for the R. argillacea species complex provide the first molecular evidence of chronic airway colonization by these fungi in CF patients.
[Mh] Termos MeSH primário: Fibrose Cística/complicações
Eurotiales/classificação
Eurotiales/isolamento & purificação
Micoses/diagnóstico
Micoses/epidemiologia
Reação em Cadeia da Polimerase/métodos
[Mh] Termos MeSH secundário: Análise por Conglomerados
Eletroforese
Eurotiales/genética
Genótipo
Seres Humanos
Técnicas Microbiológicas/métodos
Epidemiologia Molecular
Micoses/microbiologia
Sequências Repetitivas de Ácido Nucleico/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160909
[St] Status:MEDLINE


  9 / 122 MEDLINE  
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[PMID]:27527087
[Au] Autor:Steinmann J; Dittmer S; Houbraken J; Buer J; Rath PM
[Ad] Endereço:Institute of Medical Microbiology, University Hospital Essen, University Duisburg-Essen, Essen, Germany Joerg.Steinmann@uk-essen.de.
[Ti] Título:In Vitro Activity of Isavuconazole against Rasamsonia Species.
[So] Source:Antimicrob Agents Chemother;60(11):6890-6891, 2016 Nov.
[Is] ISSN:1098-6596
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The in vitro susceptibilities to the novel triazole isavuconazole and six other antifungal agents of a large collection of Rasamsonia isolates (n = 47) belonging to seven species were determined. Isavuconazole and voriconazole had no in vitro activity (MIC, >32 mg/liter) against isolates of the Rasamsonia argillacea species complex. The echinocandins were the most potent antifungal drugs against all of the isolates tested (minimum effective concentration, ≤0.19 mg/liter).
[Mh] Termos MeSH primário: Eurotiales/efeitos dos fármacos
Nitrilos/farmacologia
Piridinas/farmacologia
Triazóis/farmacologia
[Mh] Termos MeSH secundário: Antifúngicos/farmacologia
Equinocandinas/farmacologia
Eurotiales/isolamento & purificação
Seres Humanos
Testes de Sensibilidade Microbiana
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Echinocandins); 0 (Nitriles); 0 (Pyridines); 0 (Triazoles); 60UTO373KE (isavuconazole)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160817
[St] Status:MEDLINE
[do] DOI:10.1128/AAC.00742-16


  10 / 122 MEDLINE  
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[PMID]:27383606
[Au] Autor:Salgueiro AM; Evtuguin DV; Saraiva JA; Almeida F
[Ad] Endereço:CICECO, Chemistry Department, University of Aveiro, Campus de Santiago, 3810-193, Aveiro, Portugal.
[Ti] Título:High pressure-promoted xylanase treatment to enhance papermaking properties of recycled pulp.
[So] Source:Appl Microbiol Biotechnol;100(23):9885-9893, 2016 Dec.
[Is] ISSN:1432-0614
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Industrially produced bleached recycled pulp (R) comprising essentially hardwood fibres was subjected to enzymatic treatment with endo-xylanase from Thermomyces lanuginosus with or without ultra-high hydrostatic pressure (UHP) pre-treatment at 300-600 MPa for 10 min. The kinetics and the extent of enzymatic hydrolysis after UHP pre-treatment under different conditions have been evaluated by released reducing sugars and the analysis of neutral sugars in pulps, respectively. The changes in surface chemical composition of pulps were assessed by UV-vis diffuse reflectance spectroscopy. UHP-pre-treated R under optimal conditions (400 MPa), with or without posterior enzymatic treatment, was used for the production of handsheets and evaluation of its mechanical properties. It was suggested that enzymatic modification improves significantly the papermaking properties of recycled pulp. These improvements were related with selective removal of xylan bound to impurities and to aggregated cellulose fibrils on the fibre surface, thus favouring the ensuing swelling and inter-fibre bonding in paper. UHP pre-treatment and posterior enzymatic treatment revealed a synergetic effect on the mechanical properties of recycled pulp. This fact was assigned to enhanced accessibility of fibres towards xylanase and by forced hydration and favourable rearrangement of cellulosic fibrils in fibres after UHP pre-treatment. The increase of basic strength properties after UHP-promoted xylanase treatment was up to 30 % being the most pronounced for the tensile strength and the burst resistance.
[Mh] Termos MeSH primário: Endo-1,4-beta-Xilanases/metabolismo
Eurotiales/enzimologia
Pressão Hidrostática
Papel
[Mh] Termos MeSH secundário: Carboidratos/análise
Hidrólise
Cinética
Análise Espectral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carbohydrates); EC 3.2.1.8 (Endo-1,4-beta Xylanases)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170124
[Lr] Data última revisão:
170124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160708
[St] Status:MEDLINE



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