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[PMID]:28039140
[Au] Autor:Yang H; Shi P; Liu Y; Xia W; Wang X; Cao H; Ma R; Luo H; Bai Y; Yao B
[Ad] Endereço:Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China.
[Ti] Título:Loop 3 of Fungal Endoglucanases of Glycoside Hydrolase Family 12 Modulates Catalytic Efficiency.
[So] Source:Appl Environ Microbiol;83(6), 2017 Mar 15.
[Is] ISSN:1098-5336
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Glycoside hydrolase (GH) family 12 comprises enzymes with a wide range of activities critical for the degradation of lignocellulose. However, the important roles of the loop regions of GH12 enzymes in substrate specificity and catalytic efficiency remain poorly understood. This study examined how the loop 3 region affects the enzymatic properties of GH12 glucanases using EG12A from P1 and EG (PDB 1KS4) from Acidophilic and thermophilic EG12A had the highest catalytic efficiency ( / , 3,001 and 263 ml/mg/s toward lichenin and carboxymethyl cellulose sodium [CMC-Na], respectively) known so far. Based on the multiple-sequence alignment and homology modeling, two specific sequences (FN and STTQA) were identified in the loop 3 region of GH12 endoglucanases from fungi. To determine their functions, these sequences were introduced into EG12A, or the counterpart sequence STTQA was removed from EG. These modifications had no effects on the optimal pH and temperature or substrate specificity but changed the catalytic efficiency ( / ) of these enzymes (in descending order, EG12A [100%], EG12A-FN [140%], and EG12A-STTQA [190%]; EG [100%] and EGΔSTTQA [41%]). Molecular docking and dynamic simulation analyses revealed that the longer loop 3 in GH12 may strengthen the hydrogen-bond interactions between the substrate and protein, thereby increasing the turnover rate ( ). This study provides a new insight to understand the vital roles of loop 3 for GH12 endoglucanases in catalysis. Loop structures play critical roles in the substrate specificity and catalytic hydrolysis of GH12 enzymes. Three typical loops exist in these enzymes. Loops 1 and 2 are recognized as the catalytic loops and are closely related to the substrate specificity and catalytic efficiency. Loop 3 locates in the -1 or +1 subsite and varies a lot in amino acid composition, which may play a role in catalysis. In this study, two GH12 glucanases, EG12A and EG, which were mutated by introducing or deleting partial loop 3 sequences FN and/or STTQA, were selected to identify the function of loop 3. It revealed that the longer loop 3 of GH12 glucanases may strengthen the hydrogen network interactions between the substrate and protein, consequently increasing the turnover rate ( ). This study proposes a strategy to increase the catalytic efficiency of GH12 glucanases by improving the hydrogen network between substrates and catalytic loops.
[Mh] Termos MeSH primário: Aspergillus niger/enzimologia
Celulase/metabolismo
Glicosídeo Hidrolases/metabolismo
Lignina/metabolismo
Neosartorya/enzimologia
Domínios Proteicos/genética
[Mh] Termos MeSH secundário: Aspergillus niger/genética
Aspergillus niger/metabolismo
Catálise
Celulase/genética
Glucanos/metabolismo
Glicosídeo Hidrolases/genética
Ligações de Hidrogênio
Simulação de Acoplamento Molecular
Simulação de Dinâmica Molecular
Neosartorya/genética
Neosartorya/metabolismo
Especificidade por Substrato
beta-Glucanas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glucans); 0 (beta-Glucans); 11132-73-3 (lignocellulose); 9005-53-2 (Lignin); EC 3.2.1.- (Glycoside Hydrolases); EC 3.2.1.4 (Cellulase); M7F3LRN53I (lichenin)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170101
[St] Status:MEDLINE


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[PMID]:27680770
[Au] Autor:Rajachan OA; Kanokmedhakul K; Sanmanoch W; Boonlue S; Hannongbua S; Saparpakorn P; Kanokmedhakul S
[Ad] Endereço:Natural Products Research Unit, Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand.
[Ti] Título:Chevalone C analogues and globoscinic acid derivatives from the fungus Neosartorya spinosa KKU-1NK1.
[So] Source:Phytochemistry;132:68-75, 2016 Dec.
[Is] ISSN:1873-3700
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Four meroterpenoids, 1-hydroxychevalone C, 1-acetoxychevalone C, 1,11-dihydroxychevalone C, and 11-hydroxychevalone C and two ester epimers, 2S,4S-spinosate and 2S,4R-spinosate, together with seven known compounds, chevalones B, C, and E, tryptoquivaline, nortryptoquivaline, tryptoquivaline L, and quinadoline A were isolated from the fungus Neosartorya spinosa. Their structures were established based on spectroscopic data analyses. The theoretical ECD spectra of epimers, 2S,4S-spinosate and 2S,4R-spinosate were calculated to support the experimental results of their CD spectra. 1-hydroxychevalone C exhibited antimycobacterial activity against Mycobacterium tuberculosis with a MIC value of 26.4 µM. 1-Acetoxychevalone C and tryptoquivaline showed antimalarial activity against Plasmodium falciparum with IC values of 6.67 and 2.65 µM, respectively. In addition, 1-hydroxychevalone C, 1-acetoxychevalone C, 1,11-dihydroxychevalone C and quinadoline A showed cytotoxicity against KB and NCI-H187 cancer cell lines with IC values in the range of 32.7-103.3 µM.
[Mh] Termos MeSH primário: Antibacterianos/isolamento & purificação
Antimaláricos/isolamento & purificação
Antineoplásicos/isolamento & purificação
Neosartorya/química
Terpenos/isolamento & purificação
[Mh] Termos MeSH secundário: Antibacterianos/química
Antibacterianos/farmacologia
Antimaláricos/química
Antimaláricos/farmacologia
Antineoplásicos/química
Antineoplásicos/farmacologia
Ensaios de Seleção de Medicamentos Antitumorais
Seres Humanos
Indóis/química
Concentração Inibidora 50
Estrutura Molecular
Mycobacterium tuberculosis/efeitos dos fármacos
Plasmodium falciparum/efeitos dos fármacos
Terpenos/química
Terpenos/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Antimalarials); 0 (Antineoplastic Agents); 0 (Indoles); 0 (Terpenes); 0 (chevalone C); CW5S8OP3VO (tryptoquivaline)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170227
[Lr] Data última revisão:
170227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160930
[St] Status:MEDLINE


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[PMID]:27530464
[Au] Autor:Prata-Sena M; Ramos AA; Buttachon S; Castro-Carvalho B; Marques P; Dethoup T; Kijjoa A; Rocha E
[Ad] Endereço:Interdisciplinary Center for Marine and Environmental Research (CIIMAR), University of Porto (UPorto), Porto, Portugal.
[Ti] Título:Cytotoxic activity of Secondary Metabolites from Marine-derived Fungus Neosartorya siamensis in Human Cancer Cells.
[So] Source:Phytother Res;30(11):1862-1871, 2016 Nov.
[Is] ISSN:1099-1573
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Compounds isolated from the marine sea fan-derived fungus Neosartorya siamensis (KUFA 0017), namely, 2,4-dihydroxy-3-methylacetophenon (1), chevalone C (2), nortryptoquivaline (4), tryptoquivaline H (6), tryptoquivaline F (7), fiscalin A (8), epi-fiscalin A (9), epi-neofiscalin A (11) and epi-fiscalin C (13) were tested for anti-proliferative activity by MTT assay, DNA damage induction by comet assay, and induction of cell death by nuclear condensation assay on colon HCT116, liver HepG2 and melanoma A375 cancer cell lines. Compounds 2, 4, 8, 9, 11 and 13 presented IC values ranging from 24 to 153 µM in the selected cell lines. Cell death was induced in HCT116 by compounds 2, 4 and 8. In HepG2, compounds 4, 8, 9 and 11 were able to induce significant cell death. This induction of cell death is possibly not related to genotoxicity because none of the compounds induced significant DNA damage. These results suggest that selected compounds present an interesting anti-proliferative activity and cell death induction, consequently showing potential (specifically epi-fiscalin C) as future leads for chemotherapeutic agents. Further studies on mechanisms of action should ensue. Copyright © 2016 John Wiley & Sons, Ltd.
[Mh] Termos MeSH primário: Antineoplásicos/química
Neoplasias/tratamento farmacológico
Neosartorya/química
[Mh] Termos MeSH secundário: Antineoplásicos/farmacologia
Linhagem Celular Tumoral
Citotoxicidade Imunológica
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160818
[St] Status:MEDLINE
[do] DOI:10.1002/ptr.5696


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[PMID]:27503553
[Au] Autor:Sun Q; Wang H; Zhang H; Luo H; Shi P; Bai Y; Lu F; Yao B; Huang H
[Ad] Endereço:Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China; Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, College of Bioengineering, Tianjin University of Science &
[Ti] Título:Heterologous production of an acidic thermostable lipase with broad-range pH activity from thermophilic fungus Neosartorya fischeri P1.
[So] Source:J Biosci Bioeng;122(5):539-544, 2016 Nov.
[Is] ISSN:1347-4421
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Thermophilic Neosartorya fischeri P1 is an excellent lipase producer and harbors seven lipase genes. All genes were found to be functional after heterologous expression in Escherichia coli. One of them, LIP09, showed high-level expression in Pichia pastoris with the yield of 2.0 g/L in a 3.7-L fermentor. Deduced amino acid sequence of LIP09 consists of a putative signal peptide (residues 1-19) and a mature polypeptide (residues 20-562). Compared with other fungal counterparts, purified recombinant LIP09 has some superior properties. It exhibited maximum activity at 60°C and pH 5.0, had broad pH adaptability (>60% activity at pH 3.5-8.0) and stability (retaining >90% activity after incubation at pH 3.0-7.0 for 1 h at 40°C), and was highly thermostable (retaining >96% activity after incubation at 50°C for 30 min). The r-LIP09 had a preference for the medium-chain length p-nitrophenyl esters (C12) rather than short and long-chain length substrates. The high-level expression and excellent properties make LIP09 a potential enzyme candidate in food and feed industries.
[Mh] Termos MeSH primário: Proteínas de Bactérias/biossíntese
Lipase/biossíntese
Neosartorya/enzimologia
Neosartorya/genética
[Mh] Termos MeSH secundário: Adaptação Biológica/genética
Sequência de Aminoácidos
Proteínas de Bactérias/genética
Clonagem Molecular
Estabilidade Enzimática/genética
Escherichia coli
Concentração de Íons de Hidrogênio
Lipase/genética
Pichia/genética
Proteínas Recombinantes/biossíntese
Proteínas Recombinantes/genética
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Recombinant Proteins); EC 3.1.1.3 (Lipase); EC 3.1.1.3 (thermostable lipase)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160810
[St] Status:MEDLINE


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[PMID]:27447650
[Au] Autor:May Zin WW; Buttachon S; Dethoup T; Fernandes C; Cravo S; Pinto MM; Gales L; Pereira JA; Silva AM; Sekeroglu N; Kijjoa A
[Ad] Endereço:ICBAS-Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal. wwmzin.chem.yu@gmail.com.
[Ti] Título:New Cyclotetrapeptides and a New Diketopiperzine Derivative from the Marine Sponge-Associated Fungus Neosartorya glabra KUFA 0702.
[So] Source:Mar Drugs;14(7), 2016 Jul 20.
[Is] ISSN:1660-3397
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Two new cyclotetrapeptides, sartoryglabramides A (5) and B (6), and a new analog of fellutanine A (8) were isolated, together with six known compounds including ergosta-4, 6, 8 (14), 22-tetraen-3-one, ergosterol 5, 8-endoperoxide, helvolic acid, aszonalenin (1), (3R)-3-(1H-indol-3-ylmethyl)-3,4-dihydro-1H-1,4-benzodiazepine-2,5-dione (2), takakiamide (3), (11aR)-2,3-dihydro-1H-pyrrolo[2,1-c][1,4]benzodiazepine-5,11(10H,11aH)-dione (4), and fellutanine A (7), from the ethyl acetate extract of the culture of the marine sponge-associated fungus Neosartorya glabra KUFA 0702. The structures of the new compounds were established based on extensive 1D and 2D spectral analysis. X-ray analysis was also used to confirm the relative configuration of the amino acid constituents of sartoryglabramide A (5), and the absolute stereochemistry of the amino acid constituents of sartoryglabramide A (5) and sartoryglabramides B (6) was determined by chiral HPLC analysis of their hydrolysates by co-injection with the d- and l- amino acids standards. Compounds 1-8 were tested for their antibacterial activity against Gram-positive (Escherichia coli ATCC 25922) and Gram-negative (Staphyllococus aureus ATCC 25923) bacteria, as well as for their antifungal activity against filamentous (Aspergillus fumigatus ATCC 46645), dermatophyte (Trichophyton rubrum ATCC FF5) and yeast (Candida albicans ATCC 10231). None of the tested compounds exhibited either antibacterial (MIC > 256 µg/mL) or antifungal activities (MIC > 512 µg/mL).
[Mh] Termos MeSH primário: Dicetopiperazinas/química
Fungos/química
Neosartorya/química
Oligopeptídeos/química
Poríferos/química
[Mh] Termos MeSH secundário: Aminoácidos/química
Animais
Antibacterianos/química
Antibacterianos/farmacologia
Antifúngicos/química
Antifúngicos/farmacologia
Bactérias/efeitos dos fármacos
Benzodiazepinas/química
Benzodiazepinas/farmacologia
Candida albicans/efeitos dos fármacos
Dicetopiperazinas/farmacologia
Ergosterol/química
Ergosterol/farmacologia
Ácido Fusídico/análogos & derivados
Ácido Fusídico/química
Ácido Fusídico/farmacologia
Alcaloides de Indol/química
Alcaloides de Indol/farmacologia
Testes de Sensibilidade Microbiana/métodos
Oligopeptídeos/farmacologia
Estereoisomerismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Anti-Bacterial Agents); 0 (Antifungal Agents); 0 (Diketopiperazines); 0 (Indole Alkaloids); 0 (Oligopeptides); 0 (aszonalenin); 12794-10-4 (Benzodiazepines); 59XE10C19C (Fusidic Acid); MZX54GS8AH (helvolic acid); Z30RAY509F (Ergosterol)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170510
[Lr] Data última revisão:
170510
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160723
[St] Status:MEDLINE


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[PMID]:27438842
[Au] Autor:Prompanya C; Dethoup T; Gales L; Lee M; Pereira JA; Silva AM; Pinto MM; Kijjoa A
[Ad] Endereço:ICBAS-Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal. chadaporn@buu.ac.th.
[Ti] Título:New Polyketides and New Benzoic Acid Derivatives from the Marine Sponge-Associated Fungus Neosartorya quadricincta KUFA 0081.
[So] Source:Mar Drugs;14(7), 2016 Jul 16.
[Is] ISSN:1660-3397
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Two new pentaketides, including a new benzofuran-1-one derivative (1) and a new isochromen-1-one (5), and seven new benzoic acid derivatives, including two new benzopyran derivatives (2a, b), a new benzoxepine derivative (3), two new chromen-4-one derivatives (4b, 7) and two new benzofuran derivatives (6a, b), were isolated, together with the previously reported 2,3-dihydro-6-hydroxy-2,2-dimethyl-4H-1-benzopyran-4-one (4a), from the culture of the marine sponge-associated fungus Neosartorya quadricincta KUFA 0081. The structures of the new compounds were established based on 1D and 2D NMR spectral analysis, and in the case of compounds 1, 2a, 4b, 5, 6a and 7, the absolute configurations of their stereogenic carbons were determined by an X-ray crystallographic analysis. None of the isolated compounds were active in the tests for antibacterial activity against Gram-positive and Gram-negative bacteria, as well as multidrug-resistant isolates from the environment (MIC > 256 µg/mL), antifungal activity against yeast (Candida albicans ATTC 10231), filamentous fungus (Aspergillus fumigatus ATTC 46645) and dermatophyte (Trichophyton rubrum FF5) (MIC > 512 µg/mL) and in vitro growth inhibitory activity against the MCF-7 (breast adenocarcinoma), NCI-H460 (non-small cell lung cancer) and A375-C5 (melanoma) cell lines (GI50 > 150 µM) by the protein binding dye SRB method.
[Mh] Termos MeSH primário: Ácido Benzoico/química
Fungos/química
Neosartorya/química
Policetídeos/química
Poríferos/química
[Mh] Termos MeSH secundário: Animais
Antibacterianos/química
Antibacterianos/farmacologia
Antifúngicos/química
Antifúngicos/farmacologia
Arthrodermataceae/efeitos dos fármacos
Aspergillus fumigatus/efeitos dos fármacos
Bactérias/efeitos dos fármacos
Ácido Benzoico/farmacologia
Benzopiranos/química
Benzopiranos/farmacologia
Neoplasias da Mama/tratamento farmacológico
Candida albicans/efeitos dos fármacos
Carcinoma Pulmonar de Células não Pequenas/tratamento farmacológico
Linhagem Celular Tumoral
Feminino
Seres Humanos
Neoplasias Pulmonares/tratamento farmacológico
Células MCF-7
Espectroscopia de Ressonância Magnética/métodos
Melanoma/tratamento farmacológico
Testes de Sensibilidade Microbiana/métodos
Policetídeos/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Antifungal Agents); 0 (Benzopyrans); 0 (Polyketides); 8SKN0B0MIM (Benzoic Acid)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170510
[Lr] Data última revisão:
170510
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160721
[St] Status:MEDLINE


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[PMID]:27400964
[Au] Autor:Xue X; Wu Y; Qin X; Ma R; Luo H; Su X; Yao B
[Ad] Endereço:Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, No. 12 South Zhongguancun Street, Beijing, 100081, People's Republic of China.
[Ti] Título:Revisiting overexpression of a heterologous ß-glucosidase in Trichoderma reesei: fusion expression of the Neosartorya fischeri Bgl3A to cbh1 enhances the overall as well as individual cellulase activities.
[So] Source:Microb Cell Fact;15(1):122, 2016 Jul 11.
[Is] ISSN:1475-2859
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The filamentous fungus Trichoderma reesei has the capacity to secret large amounts of cellulase and is widely used in a variety of industries. However, the T. reesei cellulase is weak in ß-glucosidase activity, which results in accumulation of cellobiose inhibiting the endo- and exo-cellulases. By expressing an exogenous ß-glucosidase gene, the recombinant T. reesei cellulase is expected to degrade cellulose into glucose more efficiently. RESULTS: The thermophilic ß-glucosidase NfBgl3A from Neosartorya fischeri is chosen for overexpression in T. reesei due to its robust activity. In vitro, the Pichia pastoris-expressed NfBgl3A aided the T. reesei cellulase in releasing much more glucose with significantly lower amounts of cellobiose from crystalline cellulose. The NfBgl3A gene was hence fused to the cbh1 structural gene and assembled between the strong cbh1 promoter and cbh1 terminator to obtain pRS-NfBgl3A by using the DNA assembler method. pRS-NfBgl3A was transformed into the T. reesei uridine auxotroph strain TU-6. Six positive transformants showed ß-glucosidase activities of 2.3-69.7 U/mL (up to 175-fold higher than that of wild-type). The largely different ß-glucosidase activities in the transformants may be ascribed to the gene copy numbers of NfBgl3A or its integration loci. The T. reesei-expressed NfBgl3A showed highly similar biochemical properties to that expressed in P. pastoris. As expected, overexpression of NfBgl3A enhanced the overall cellulase activity of T. reesei. The CBHI activity in all transformants increased, possibly due to the extra copies of cbh1 gene introduced, while the endoglucanase activity in three transformants also largely increased, which was not observed in any other studies overexpressing a ß-glucosidase. NfBgl3A had significant transglycosylation activity, generating sophorose, a potent cellulase inducer, and other oligosaccharides from glucose and cellobiose. CONCLUSIONS: We report herein the successful overexpression of a thermophilic N. fischeri ß-glucosidase in T. reesei. In the same time, the fusion of NfBgl3A to the cbh1 gene introduced extra copies of the cellobiohydrolase 1 gene. As a result, we observed improved ß-glucosidase and cellobiohydrolase activity as well as the overall cellulase activity. In addition, the endoglucanase activity also increased in some of the transformants. Our results may shed light on design of more robust T. reesei cellulases.
[Mh] Termos MeSH primário: Celulase/metabolismo
Proteínas Fúngicas/genética
Neosartorya/enzimologia
Proteínas Recombinantes de Fusão/genética
Trichoderma/genética
beta-Glucosidase/genética
[Mh] Termos MeSH secundário: Celobiose/metabolismo
Celulase/genética
Celulose/metabolismo
Proteínas Fúngicas/metabolismo
Regulação Fúngica da Expressão Gênica
Glucose/metabolismo
Neosartorya/genética
Regiões Promotoras Genéticas
Proteínas Recombinantes de Fusão/metabolismo
Trichoderma/metabolismo
beta-Glucosidase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungal Proteins); 0 (Recombinant Fusion Proteins); 16462-44-5 (Cellobiose); 9004-34-6 (Cellulose); EC 3.2.1.21 (beta-Glucosidase); EC 3.2.1.4 (Cellulase); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170313
[Lr] Data última revisão:
170313
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160713
[St] Status:MEDLINE
[do] DOI:10.1186/s12934-016-0520-9


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[PMID]:26972831
[Au] Autor:Bilovol Y; Panaccione DG
[Ad] Endereço:Genetics and Developmental Biology Program, Division of Plant and Soil Sciences, West Virginia University, Morgantown, WV, 26506, USA.
[Ti] Título:Functional analysis of the gene controlling hydroxylation of festuclavine in the ergot alkaloid pathway of Neosartorya fumigata.
[So] Source:Curr Genet;62(4):853-860, 2016 Nov.
[Is] ISSN:1432-0983
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bioactive ergot alkaloids produced by several species of fungi are important molecules in agriculture and medicine. Much of the ergot alkaloid pathway has been elucidated, but a few steps, including the gene controlling hydroxylation of festuclavine to fumigaclavine B, remain unsolved. Festuclavine is a key intermediate in the fumigaclavine branch of the ergot alkaloid pathway of the opportunistic pathogen Neosartorya fumigata and also in the dihydrolysergic acid-based ergot alkaloid pathway of certain Claviceps species. Based on several lines of evidence, the N. fumigata gene easM is a logical candidate to encode the festuclavine-hydroxylating enzyme. To test this hypothesis we disrupted easM function by replacing part of its coding sequences with a hygromycin resistance gene and transforming N. fumigata with this construct. High-pressure liquid chromatography analysis demonstrated that easM deletion mutants were blocked in the ergot alkaloid pathway at festuclavine, and downstream products were eliminated. An additional alkaloid, proposed to be a prenylated form of festuclavine on the basis of mass spectral data, also accumulated to higher concentrations in the easM knockout. Complementation with the wild-type allele of easM gene restored the ability of the fungus to produce downstream compounds. These results indicate that easM encodes an enzyme required for fumigaclavine B synthesis likely by hydroxylating festuclavine. The festuclavine-accumulating strain of N. fumigata may facilitate future investigations of the biosynthesis of dihydrolysergic acid derivatives, which are derived from festuclavine and are the basis for several important drugs.
[Mh] Termos MeSH primário: Ergolinas/metabolismo
Alcaloides de Claviceps/metabolismo
Genes Fúngicos
Redes e Vias Metabólicas
Neosartorya/genética
Neosartorya/metabolismo
[Mh] Termos MeSH secundário: Técnicas de Inativação de Genes
Teste de Complementação Genética
Hidroxilação
Família Multigênica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ergolines); 0 (Ergot Alkaloids); 436-41-9 (costaclavin)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160315
[St] Status:MEDLINE


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Texto completo SciELO Chile
[PMID]:26965879
[Au] Autor:Cruz R; Piontelli E
[Ti] Título:[Neosartorya fischeri (Wehmer) Malloch & Cain].
[Ti] Título:Neosartorya fischeri (Wehmer) Malloch & Cain..
[So] Source:Rev Chilena Infectol;33(1):59-60, 2016 Feb.
[Is] ISSN:0717-6341
[Cp] País de publicação:Chile
[La] Idioma:spa
[Mh] Termos MeSH primário: Neosartorya
[Mh] Termos MeSH secundário: Neosartorya/classificação
Neosartorya/citologia
Neosartorya/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1608
[Cu] Atualização por classe:160311
[Lr] Data última revisão:
160311
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160312
[St] Status:MEDLINE


  10 / 88 MEDLINE  
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[PMID]:26935258
[Au] Autor:Zin WW; Prompanya C; Buttachon S; Kijjoa A
[Ti] Título:Bioactive Secondary Metabolites from a Thai Collection of Soil and Marine-Derived Fungi of the Genera Neosartorya and Aspergillus.
[So] Source:Curr Drug Deliv;13(3):378-88, 2016.
[Is] ISSN:1875-5704
[Cp] País de publicação:United Arab Emirates
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Fungi are microorganisms which can produce interesting secondary metabolites with structural diversity. Although terrestrial fungi have been extensively investigated for their bioactive secondary metabolites such as antibiotics, marine-derived fungi have only recently attracted attention of Natural Products chemists. METHODS: Our group has been working on the secondary metabolites produced by the cultures of the fungi of the genera Neosartorya and Aspergillus, collected from soil and marine environments from the tropical region for the purpose of finding new leads for anticancer and antibacterial drugs. RESULTS: This review covers only the secondary metabolites of four soil and six marine-derived species of Neosarorya as well as a new species of marine-derived Aspergillus, investigated by our group. In total, we have isolated fifty three secondary metabolites which can be categorized as polyketides (two), isocoumarins (six), terpenoids (two), meroterpenes (fourteen), alkaloids (twenty eight) and cyclic peptide (one). The anticancer and antibacterial activities of these fungal metabolites are also discussed. CONCLUSION: Among fifty three secondary metabolites isolated, only the alkaloid eurochevalierine and the cadinene sesquiterpene, isolated from the soil fungus N. pseudofisheri, showed relevant in vitro cytostatic activity against glioblastoma (U373) and non-small cell lung cancer (A549) cell lines while the meroditerpene aszonapyrone A exhibited strong antibacterial activity against multidrug-resistant Gram-positive bacteria and also strong antibiofilm activity in these isolates.
[Mh] Termos MeSH primário: Aspergillus/metabolismo
Produtos Biológicos/metabolismo
Neosartorya/metabolismo
[Mh] Termos MeSH secundário: Antibacterianos/metabolismo
Antibacterianos/farmacologia
Antineoplásicos/metabolismo
Antineoplásicos/farmacologia
Produtos Biológicos/farmacologia
Seres Humanos
Água do Mar/microbiologia
Microbiologia do Solo
Tailândia
Microbiologia da Água
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Antineoplastic Agents); 0 (Biological Products)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170215
[Lr] Data última revisão:
170215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160304
[St] Status:MEDLINE



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