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  1 / 1169 MEDLINE  
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[PMID]:28562088
[Au] Autor:Yoshida S; Suzuki T; Furuno H; Aboshi T; Murayama T; Koseki T; Shiono Y
[Ad] Endereço:a Faculty of Agriculture, Department of Food, Life, and Environmental Science , Yamagata University , Yamagata , Japan.
[Ti] Título:Spectroscopic characterisation of two polyketide metabolites from Cylindrocarpon sp. from driftwood.
[So] Source:Nat Prod Res;32(1):60-64, 2018 Jan.
[Is] ISSN:1478-6427
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Two new polyketides, (5R,7R,9R)-7,9-dihydroxy-5-decanolide and (4E,6R,9R)- 6,9-dihydroxydec-4-enoic acid (2), were isolated from rice cultures of Cylindrocarpon sp. SY-39 discovered during screening of driftwood at a Shonai coast area in Yamagata, Japan. The structures of 1 and 2 were determined using a variety of spectroscopic methods. Compound 2 exhibited moderate antimicrobial activity against Staphylococcus aureus NBRC 13276 and Aspergillus clavatus F 318a at a concentration of 50 µg per disk.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Antifúngicos/farmacologia
Hypocreales/química
Policetídeos/química
[Mh] Termos MeSH secundário: Antibacterianos/química
Antifúngicos/química
Aspergillus/efeitos dos fármacos
Japão
Espectroscopia de Ressonância Magnética
Estrutura Molecular
Staphylococcus aureus/efeitos dos fármacos
Estereoisomerismo
Madeira/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Antifungal Agents); 0 (Polyketides)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170601
[St] Status:MEDLINE
[do] DOI:10.1080/14786419.2017.1332616


  2 / 1169 MEDLINE  
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[PMID]:28467379
[Au] Autor:Zhong J; Cheng CY; Gao BD; Zhou Q; Zhu HJ
[Ad] Endereço:Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Nongda Road 1, Furong District, Changsha 410128, China. wzzhtx@sina.com.
[Ti] Título:Mycoviruses in the Plant Pathogen Ustilaginoidea virens Are Not Correlated with the Genetic Backgrounds of Its Hosts.
[So] Source:Int J Mol Sci;18(5), 2017 May 03.
[Is] ISSN:1422-0067
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:, the causal agent of rice false smut, is one of the most devastating grain diseases that causes loss of yield in most rice-growing areas worldwide. In this study, we performed a dsRNA screen to isolate mycoviruses from 35 strains. The results revealed that 34 of the tested isolates were infected by various dsRNA elements, displaying highly viral diversity and mixed infections. We characterized a 5.3 kbp dsRNA from a typical isolate containing dsRNA segments with sizes ranging from 0.5 to 5.3 kbp. Sequence analysis of its genomic properties indicated that it is a novel victorivirus, named RNA virus 5 (UvRV5), that belongs to the family . RT-PCR detection was performed and indicated that not all the dsRNA bands that were 5.3 kbp in size contained UvRV5. Moreover, the genetic relatedness of all the strains was estimated according to phylogenetic analysis of the partial intergenic spacer region (IGS) sequences. However, concordance was not found between the dsRNA profiles and the IGS-based genetic relatedness of their host fungi.
[Mh] Termos MeSH primário: Micovírus/genética
Interações Hospedeiro-Patógeno/genética
Hypocreales/genética
Hypocreales/virologia
Oryza/microbiologia
Doenças das Plantas/microbiologia
Totiviridae/genética
[Mh] Termos MeSH secundário: Patrimônio Genético
Genoma Fúngico
Genoma Viral
Filogenia
RNA de Cadeia Dupla/isolamento & purificação
Análise de Sequência de RNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Double-Stranded)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE


  3 / 1169 MEDLINE  
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[PMID]:29304036
[Au] Autor:Oren E; Klingeman W; Gazis R; Moulton J; Lambdin P; Coggeshall M; Hulcr J; Seybold SJ; Hadziabdic D
[Ad] Endereço:Department of Entomology and Plant Pathology, University of Tennessee, Knoxville, TN, United States of America.
[Ti] Título:A novel molecular toolkit for rapid detection of the pathogen and primary vector of thousand cankers disease.
[So] Source:PLoS One;13(1):e0185087, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Thousand Cankers Disease (TCD) of Juglans and Pterocarya (Juglandaceae) involves a fungal pathogen, Geosmithia morbida, and a primary insect vector, Pityophthorus juglandis. TCD was described originally from dying Juglans nigra trees in the western United States (USA), but it was reported subsequently from the eastern USA and northern Italy. The disease is often difficult to diagnose due to the absence of symptoms or signs on the bark surface of the host. Furthermore, disease symptoms can be confused with those caused by other biotic and abiotic agents. Thus, there is a critical need for a method for rapid detection of the pathogen and vector of TCD. Using species-specific microsatellite DNA markers, we developed a molecular protocol for the detection of G. morbida and P. juglandis. To demonstrate the utility of the method for delineating TCD quarantine zones, we tested whether geographical occurrence of symptoms and signs of TCD was correlated with molecular evidence for the presence of the cryptic TCD organisms. A total of 1600 drill cores were taken from branch sections collected from three regions (n = 40 trees for each location): California-J. hindsii (heavy disease incidence); Tennessee-J. nigra (mild disease incidence); and outside the known TCD zone (Missouri-J. nigra, no record of the disease). California samples had the highest incidence of the TCD organisms (85%, 34/40). Tennessee had intermediate incidence (42.5%, 17/40), whereas neither organism was detected in samples from Missouri. The low cost molecular protocol developed here has a high degree of sensitivity and specificity, and it significantly reduces sample-processing time, making the protocol a powerful tool for rapid detection of TCD.
[Mh] Termos MeSH primário: Hypocreales/genética
Hypocreales/patogenicidade
Insetos Vetores/genética
Insetos Vetores/microbiologia
Juglans/microbiologia
Doenças das Plantas/microbiologia
Gorgulhos/genética
Gorgulhos/microbiologia
[Mh] Termos MeSH secundário: Animais
California
DNA Fúngico/genética
Técnicas Genéticas
Hypocreales/isolamento & purificação
Repetições de Microssatélites
Missouri
Especificidade da Espécie
Tennessee
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Fungal)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180106
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185087


  4 / 1169 MEDLINE  
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[PMID]:29023475
[Au] Autor:Wang D; Li L; Wu G; Vasseur L; Yang G; Huang P
[Ad] Endereço:Tea Research Institute, Fujian Academy of Agricultural Sciences, Fu'an, Fujian, China.
[Ti] Título:De novo transcriptome sequencing of Isaria cateniannulata and comparative analysis of gene expression in response to heat and cold stresses.
[So] Source:PLoS One;12(10):e0186040, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Isaria cateniannulata is a very important and virulent entomopathogenic fungus that infects many insect pest species. Although I. cateniannulata is commonly exposed to extreme environmental temperature conditions, little is known about its molecular response mechanism to temperature stress. Here, we sequenced and de novo assembled the transcriptome of I. cateniannulata in response to high and low temperature stresses using Illumina RNA-Seq technology. Our assembly encompassed 17,514 unigenes (mean length = 1,197 bp), in which 11,445 unigenes (65.34%) showed significant similarities to known sequences in NCBI non-redundant protein sequences (Nr) database. Using digital gene expression analysis, 4,483 differentially expressed genes (DEGs) were identified after heat treatment, including 2,905 up-regulated genes and 1,578 down-regulated genes. Under cold stress, 1,927 DEGs were identified, including 1,245 up-regulated genes and 682 down-regulated genes. The expression patterns of 18 randomly selected candidate DEGs resulting from quantitative real-time PCR (qRT-PCR) were consistent with their transcriptome analysis results. Although DEGs were involved in many pathways, we focused on the genes that were involved in endocytosis: In heat stress, the pathway of clathrin-dependent endocytosis (CDE) was active; however at low temperature stresses, the pathway of clathrin-independent endocytosis (CIE) was active. Besides, four categories of DEGs acting as temperature sensors were observed, including cell-wall-major-components-metabolism-related (CWMCMR) genes, heat shock protein (Hsp) genes, intracellular-compatible-solutes-metabolism-related (ICSMR) genes and glutathione S-transferase (GST). These results enhance our understanding of the molecular mechanisms of I. cateniannulata in response to temperature stresses and provide a valuable resource for the future investigations.
[Mh] Termos MeSH primário: Resposta ao Choque Frio/genética
Regulação Fúngica da Expressão Gênica
Resposta ao Choque Térmico/genética
Hypocreales/fisiologia
[Mh] Termos MeSH secundário: Perfilação da Expressão Gênica
Ontologia Genética
Hypocreales/genética
Reação em Cadeia da Polimerase Via Transcriptase Reversa
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171013
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186040


  5 / 1169 MEDLINE  
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[PMID]:28981527
[Au] Autor:Dymarska M; Grzeszczuk J; Urbaniak M; Janeczko T; Plaskowska E; Stepien L; Kostrzewa-Suslow E
[Ad] Endereço:Department of Chemistry, Faculty of Biotechnology and Food Science, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland.
[Ti] Título:Glycosylation of 6-methylflavone by the strain Isaria fumosorosea KCH J2.
[So] Source:PLoS One;12(10):e0184885, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Entomopathogenic fungi are known for their ability to carry out glycosylation of flavonoids, which usually results in the improvement of their stability and bioavailability. In this study we used a newly isolated strain of the entomopathogenic filamentous fungus Isaria fumosorosea KCH J2 as a biocatalyst. Our aim was to evaluate its ability to carry out the biotransformation of flavonoids and to obtain new flavonoid derivatives. The fungus was isolated from a spider's carcass and molecularly identified using analysis of the ITS1-ITS2 rDNA sequence. As a result of biotransformation of 6-methylflavone two new products were obtained: 6-methylflavone 8-O-ß-D-(4"-O-methyl)-glucopyranoside and 6-methylflavone 4'-O-ß-D-(4"-O-methyl)-glucopyranoside. Chemical structures of the products were determined based on spectroscopic methods (1H NMR, 13C NMR, COSY, HMBC, HSQC). Our research allowed us to discover a new species of filamentous fungus capable of carrying out glycosylation reactions and proved that I. fumosorosea KCH J2 is an effective biocatalyst for glycosylation of flavonoid compounds. For the first time we describe biotransformations of 6-methylflavone and the attachment of the sugar unit to the flavonoid substrate having no hydroxyl group. The possibility of using flavonoid aglycones is often limited by their low bioavailability due to poor solubility in water. The incorporation of a sugar unit improves the physical properties of tested compounds and thus increases the chance of using them as pharmaceuticals.
[Mh] Termos MeSH primário: Flavonas/metabolismo
Hypocreales/metabolismo
[Mh] Termos MeSH secundário: Biotransformação
Espectroscopia de Ressonância Magnética Nuclear de Carbono-13
Cromatografia Líquida de Alta Pressão
Glicosilação
Espectroscopia de Prótons por Ressonância Magnética
Espectrofotometria Ultravioleta
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (6-methylflavone); 0 (Flavones)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171026
[Lr] Data última revisão:
171026
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171006
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184885


  6 / 1169 MEDLINE  
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[PMID]:28772192
[Au] Autor:Sica VP; Rees ER; Raja HA; Rivera-Chávez J; Burdette JE; Pearce CJ; Oberlies NH
[Ad] Endereço:Department of Chemistry and Biochemistry, University of North Carolina at Greensboro, Greensboro, NC 27402, United States.
[Ti] Título:In situ mass spectrometry monitoring of fungal cultures led to the identification of four peptaibols with a rare threonine residue.
[So] Source:Phytochemistry;143:45-53, 2017 Nov.
[Is] ISSN:1873-3700
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Peptaibols are an intriguing class of fungal metabolites due both to their wide range of reported bioactivities and to the structural variability that can be generated by the exchange of variable amino acid building blocks. In an effort to streamline the discovery of structurally diverse peptaibols, a mass spectrometry surface sampling technique was applied to screen the chemistry of fungal cultures in situ. Four previously undescribed peptaibols, all containing a rare threonine residue, were identified from a fungal culture (MSX53554), which was identified as Nectriopsis Maire (Bionectriaceae, Hypocreales, Ascomycota). These compounds not only increased the known threonine-containing peptaibols by nearly 20%, but also, the threonine residue was situated in a unique place compared to the other reported threonine-containing peptaibols. After the initial in situ detection and characterization, a large-scale solid fermentation culture was grown. The four peptaibols were isolated and characterized by mass spectrometry. In addition, one of the peptaibols was fully characterized by NMR and amino acid analysis using Marfey's reagent and exhibited moderate in vitro anticancer activity.
[Mh] Termos MeSH primário: Hypocreales/química
Peptaibols/química
Peptaibols/isolamento & purificação
Treonina/química
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Aminoácidos/metabolismo
Antibacterianos/química
Estrutura Molecular
Ressonância Magnética Nuclear Biomolecular
Trichoderma/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Anti-Bacterial Agents); 0 (Peptaibols); 2ZD004190S (Threonine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170804
[St] Status:MEDLINE


  7 / 1169 MEDLINE  
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[PMID]:28700638
[Au] Autor:Ashrafi S; Helaly S; Schroers HJ; Stadler M; Richert-Poeggeler KR; Dababat AA; Maier W
[Ad] Endereço:Institute for Epidemiology and Pathogen Diagnostics, Julius Kühn-Institut (JKI)-Federal Research Centre for Cultivated Plants, Braunschweig, Germany.
[Ti] Título:Ijuhya vitellina sp. nov., a novel source for chaetoglobosin A, is a destructive parasite of the cereal cyst nematode Heterodera filipjevi.
[So] Source:PLoS One;12(7):e0180032, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cyst nematodes are globally important pathogens in agriculture. Their sedentary lifestyle and long-term association with the roots of host plants render cyst nematodes especially good targets for attack by parasitic fungi. In this context fungi were specifically isolated from nematode eggs of the cereal cyst nematode Heterodera filipjevi. Here, Ijuhya vitellina (Ascomycota, Hypocreales, Bionectriaceae), encountered in wheat fields in Turkey, is newly described on the basis of phylogenetic analyses, morphological characters and life-style related inferences. The species destructively parasitises eggs inside cysts of H. filipjevi. The parasitism was reproduced in in vitro studies. Infected eggs were found to harbour microsclerotia produced by I. vitellina that resemble long-term survival structures also known from other ascomycetes. Microsclerotia were also formed by this species in pure cultures obtained from both, solitarily isolated infected eggs obtained from fields and artificially infected eggs. Hyphae penetrating the eggshell colonised the interior of eggs and became transformed into multicellular, chlamydospore-like structures that developed into microsclerotia. When isolated on artificial media, microsclerotia germinated to produce multiple emerging hyphae. The specific nature of morphological structures produced by I. vitellina inside nematode eggs is interpreted as a unique mode of interaction allowing long-term survival of the fungus inside nematode cysts that are known to survive periods of drought or other harsh environmental conditions. Generic classification of the new species is based on molecular phylogenetic inferences using five different gene regions. I. vitellina is the only species of the genus known to parasitise nematodes and produce microsclerotia. Metabolomic analyses revealed that within the Ijuhya species studied here, only I. vitellina produces chaetoglobosin A and its derivate 19-O-acetylchaetoglobosin A. Nematicidal and nematode-inhibiting activities of these compounds have been demonstrated suggesting that the production of these compounds may represent an adaptation to nematode parasitism.
[Mh] Termos MeSH primário: Hypocreales/patogenicidade
Alcaloides de Indol/metabolismo
[Mh] Termos MeSH secundário: Animais
Hifas/crescimento & desenvolvimento
Hypocreales/classificação
Hypocreales/genética
Hypocreales/metabolismo
Oócitos/microbiologia
Filogenia
Tylenchoidea/crescimento & desenvolvimento
Tylenchoidea/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Indole Alkaloids); 50335-03-0 (chaetoglobosins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170713
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0180032


  8 / 1169 MEDLINE  
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[PMID]:28578573
[Au] Autor:Liang X; Nong XH; Huang ZH; Qi SH
[Ad] Endereço:Key Laboratory of Tropical Marine Bio-resources and Ecology/Guangdong Key Laboratory of Marine Materia Medica/RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences , 164 West Xingang Road, Guangzhou, 510301 Guangdong, China.
[Ti] Título:Antifungal and Antiviral Cyclic Peptides from the Deep-Sea-Derived Fungus Simplicillium obclavatum EIODSF 020.
[So] Source:J Agric Food Chem;65(25):5114-5121, 2017 Jun 28.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A new linear peptide simplicilliumtide I (1) and four new cyclic peptides simplicilliumtides J-M (2-5) together with known analogues verlamelins A and B (6 and 7) were isolated from the deep-sea-derived fungal strain Simplicillium obclavatum EIODSF 020. Their structures were elucidated by spectroscopic analysis, and their absolute configurations were further confirmed by chemical structural modification, Marfey's and Mosher's methods. Compounds 2, 6, and 7 showed significant antifungal activity toward Aspergillus versicolor and Curvularia australiensis and also had obvious antiviral activity toward HSV-1 with IC values of 14.0, 16.7, and 15.6 µM, respectively. The structure-bioactivity relationship of this type of cyclic peptide was also discussed. This is the first time to discuss the effects of the lactone linkage and the substituent group of the fatty acid chain fragment on the bioactivity of this type of cyclic peptides. This is also the first time to report the antiviral activity of these cyclic peptides.
[Mh] Termos MeSH primário: Antifúngicos/farmacologia
Antivirais/farmacologia
Hypocreales/química
Peptídeos Cíclicos/farmacologia
Água do Mar/microbiologia
[Mh] Termos MeSH secundário: Antifúngicos/química
Antifúngicos/isolamento & purificação
Antivirais/química
Antivirais/isolamento & purificação
Ascomicetos/efeitos dos fármacos
Ascomicetos/crescimento & desenvolvimento
Herpesvirus Humano 1/efeitos dos fármacos
Herpesvirus Humano 1/crescimento & desenvolvimento
Hypocreales/genética
Hypocreales/isolamento & purificação
Hypocreales/metabolismo
Estrutura Molecular
Peptídeos Cíclicos/química
Peptídeos Cíclicos/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Antiviral Agents); 0 (Peptides, Cyclic)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170707
[Lr] Data última revisão:
170707
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170606
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b01238


  9 / 1169 MEDLINE  
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[PMID]:28574707
[Au] Autor:Sun W; Wang A; Xu D; Wang W; Meng J; Dai J; Liu Y; Lai D; Zhou L
[Ad] Endereço:Key Laboratory of Pest Monitoring and Green Management of Ministry of Agriculture, Department of Plant Pathology, College of Plant Protection, China Agricultural University , Beijing 100193, People's Republic of China.
[Ti] Título:New Ustilaginoidins from Rice False Smut Balls Caused by Villosiclava virens and Their Phytotoxic and Cytotoxic Activities.
[So] Source:J Agric Food Chem;65(25):5151-5160, 2017 Jun 28.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Ustilaginoidins are a class of bis-naphtho-γ-pyrones, typically produced by Villosiclava virens, the pathogen of the rice false smut (RFS), which has been one of the most destructive rice fungal diseases. Previously, we found that ustilaginoidins identified from the culture of V. virens on rice medium were less polar than those reported from the RFS balls in general. In this study, we reinvestigated the high-performance liquid chromatography with diode array detection and high-resolution mass spectrometry (HPLC-DAD-HRMS) profile of the ethyl acetate (EtOAc) extract of the RFS balls and found several interesting peaks that correspond to new ustilaginoidins. As a result, eight new and polar congeners, named ustilaginoidins Q-T (1-4), 2,3-dihydroustilaginoidin T (5), and ustilaginoidins U-W (6-8), were isolated. In addition, 17 known ustilaginoidins, including ustilaginoidins K-N (9-12), ustilaginoidin P (13), ustilaginoidin E (14), isochaetochromin B (15), and ustilaginoidins A-J (16-25), were re-isolated. The structures of the new compounds were elucidated by comprehensive analysis of the spectroscopic data. Ustilaginoidins Q (1) and R (2) feature an uncommon 2-hydroxypropyl-substituted skeleton and biogenetically incorporate one more acetate unit than common ustilaginoidins. Ustilaginoidin W (8) is a rare formate-containing bis-naphtho-γ-pyrone. Ustilaginoidins R (2), U (6), B (17), and I (24) showed moderate inhibitory activities toward the radicle or germ elongation of rice seeds. Ustilaginoidins R (2), S (3), V (7), W (8), B (17), C (18), and H-J (23-25) were cytotoxic to the tested human cancer cell lines (HCT116, NCI-H1650, BGC823, Daoy, and HepG2), with IC values in the range of 4.06-44.1 µM.
[Mh] Termos MeSH primário: Citotoxinas/toxicidade
Hypocreales/química
Micotoxinas/toxicidade
Oryza/microbiologia
Doenças das Plantas/microbiologia
[Mh] Termos MeSH secundário: Linhagem Celular Tumoral
Proliferação Celular/efeitos dos fármacos
Cromatografia Líquida de Alta Pressão
Citotoxinas/química
Citotoxinas/isolamento & purificação
Seres Humanos
Hypocreales/fisiologia
Estrutura Molecular
Micotoxinas/química
Micotoxinas/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytotoxins); 0 (Mycotoxins)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170707
[Lr] Data última revisão:
170707
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170603
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b01791


  10 / 1169 MEDLINE  
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[PMID]:28438205
[Au] Autor:Yang WC; Hsu TC; Cheng KC; Liu JR
[Ad] Endereço:Graduate Institute of Food Science and Technology, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Taipei, 10617, Taiwan.
[Ti] Título:Expression of the Clonostachys rosea lactonohydrolase gene by Lactobacillus reuteri to increase its zearalenone-removing ability.
[So] Source:Microb Cell Fact;16(1):69, 2017 Apr 24.
[Is] ISSN:1475-2859
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Mycotoxins are secondary metabolites produced by filamentous fungi that can contaminate agricultural crops in the field as well as during harvest, transportation, processing, or storage. Zearalenone (ZEN), a non-steroidal estrogenic mycotoxin, produced by Fusarium species, has been shown to be associated with reproductive disorders in farm animals and to a lesser extent in hyperoestrogenic syndromes in humans. Thus, the decontamination of ZEN in foods and feeds is an important issue. RESULTS: In this study, the gene encoding ZHD101, a ZEN-degrading enzyme produced by Clonostachys rosea IFO 7063, was cloned into an Escherichia coli-Lactobacillus shuttle vector, pNZ3004, and the resultant plasmid pNZ-zhd101 was then introduced via electroporation into Lactobacillus reuteri Pg4, a probiotic strain isolated from the gastrointestinal tract of broilers. The transformed strain L. reuteri pNZ-zhd101 acquired the capacity to degrade ZEN. In addition, the production of recombinant ZHD101 did not affect cell growth, acid and bile salt tolerance, and had only a minor effect on the adhesion ability of L. reuteri pNZ-zhd101. CONCLUSIONS: To the best of our knowledge, this is the first report of successful expression of a ZEN-degrading enzyme by intestinal lactobacilli.
[Mh] Termos MeSH primário: Hidrolases/genética
Hidrolases/metabolismo
Hypocreales/enzimologia
Lactobacillus reuteri/genética
Lactobacillus reuteri/metabolismo
Zearalenona/metabolismo
[Mh] Termos MeSH secundário: Animais
Aderência Bacteriana
Galinhas/microbiologia
Clonagem Molecular
Escherichia coli/genética
Trato Gastrointestinal/microbiologia
Expressão Gênica
Vetores Genéticos
Hypocreales/genética
Lactobacillus reuteri/enzimologia
Lactobacillus reuteri/crescimento & desenvolvimento
Lactonas
Probióticos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Lactones); 5W827M159J (Zearalenone); EC 3.- (Hydrolases)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171108
[Lr] Data última revisão:
171108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170426
[St] Status:MEDLINE
[do] DOI:10.1186/s12934-017-0687-8



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