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[PMID]:28372538
[Au] Autor:Oeser B; Kind S; Schurack S; Schmutzer T; Tudzynski P; Hinsch J
[Ad] Endereço:Institut für Biologie und Biotechnologie der Pflanzen, Westfälische Wilhelms-Universität, D-48143, Münster, Germany.
[Ti] Título:Cross-talk of the biotrophic pathogen Claviceps purpurea and its host Secale cereale.
[So] Source:BMC Genomics;18(1):273, 2017 04 04.
[Is] ISSN:1471-2164
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The economically important Ergot fungus Claviceps purpurea is an interesting biotrophic model system because of its strict organ specificity (grass ovaries) and the lack of any detectable plant defense reactions. Though several virulence factors were identified, the exact infection mechanisms are unknown, e.g. how the fungus masks its attack and if the host detects the infection at all. RESULTS: We present a first dual transcriptome analysis using an RNA-Seq approach. We studied both, fungal and plant gene expression in young ovaries infected by the wild-type and two virulence-attenuated mutants. We can show that the plant recognizes the fungus, since defense related genes are upregulated, especially several phytohormone genes. We present a survey of in planta expressed fungal genes, among them several confirmed virulence genes. Interestingly, the set of most highly expressed genes includes a high proportion of genes encoding putative effectors, small secreted proteins which might be involved in masking the fungal attack or interfering with host defense reactions. As known from several other phytopathogens, the C. purpurea genome contains more than 400 of such genes, many of them clustered and probably highly redundant. Since the lack of effective defense reactions in spite of recognition of the fungus could very well be achieved by effectors, we started a functional analysis of some of the most highly expressed candidates. However, the redundancy of the system made the identification of a drastic effect of a single gene most unlikely. We can show that at least one candidate accumulates in the plant apoplast. Deletion of some candidates led to a reduced virulence of C. purpurea on rye, indicating a role of the respective proteins during the infection process. CONCLUSIONS: We show for the first time that- despite the absence of effective plant defense reactions- the biotrophic pathogen C. purpurea is detected by its host. This points to a role of effectors in modulation of the effective plant response. Indeed, several putative effector genes are among the highest expressed genes in planta.
[Mh] Termos MeSH primário: Claviceps/genética
Flores/microbiologia
Doenças das Plantas/microbiologia
Secale/microbiologia
[Mh] Termos MeSH secundário: Claviceps/metabolismo
Resistência à Doença/genética
Flores/genética
Flores/metabolismo
Regulação Fúngica da Expressão Gênica
Regulação da Expressão Gênica de Plantas
Genes Fúngicos
Genes de Plantas
Interações Hospedeiro-Patógeno
Secale/genética
Secale/metabolismo
Transcriptoma
Fatores de Virulência/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Virulence Factors)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171120
[Lr] Data última revisão:
171120
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170405
[St] Status:MEDLINE
[do] DOI:10.1186/s12864-017-3619-4


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[PMID]:28350362
[Au] Autor:Dänicke S
[Ad] Endereço:Institute of Animal Nutrition, Friedrich-Loeffler-Institute (FLI), Federal Research Institute for Animal Health, Bundesallee 50, 38116 Braunschweig, Germany. sven.daenicke@fli.de.
[Ti] Título:Ergot Alkaloids in Fattening Chickens (Broilers): Toxic Effects and Carry over Depending on Dietary Fat Proportion and Supplementation with Non-Starch-Polysaccharide (NSP) Hydrolyzing Enzymes.
[So] Source:Toxins (Basel);9(4), 2017 Mar 28.
[Is] ISSN:2072-6651
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Ergot alkaloids (EA) are mycotoxins produced by . EA-toxicity is poorly characterized for fattening chickens. Therefore, a dose-response study was performed to identify the lowest, and no observed adverse effect levels (LOAEL and NOAEL, respectively) based on several endpoints. Non-starch-polysaccharide (NSP) cleaving enzyme addition and dietary fat content were additionally considered as factors potentially influencing EA-toxicity. Feed intake was proven to respond most sensitively to the EA presence in the diets. This sensitivity appeared to be time-dependent. While LOAEL corresponded to a total dietary EA content of 5.7 mg/kg until Day 14 of age, it decreased to 2.03 mg/kg when birds were exposed for a period of 35 days. Consequently, NOAEL corresponded to an EA content of 2.49 mg/kg diet until Day 14 of age, while 1.94 mg/kg diet applied until Day 35 of age. Liver lesions indicating enzyme activities in serum were increased after 14 days of exposure. Dietary fat content and NSP-enzyme supplementation modified EA toxicity in an interactive manner. The EA residues in serum, bile, liver and breast meat were <5 ng/g suggesting a negligible carry over of intact EA.
[Mh] Termos MeSH primário: Ração Animal/toxicidade
Alcaloides de Claviceps/toxicidade
[Mh] Termos MeSH secundário: Alanina Transaminase/sangue
Animais
Aspartato Aminotransferases/sangue
Bile/química
Galinhas/sangue
Galinhas/crescimento & desenvolvimento
Galinhas/metabolismo
Claviceps
Dieta/veterinária
Gorduras na Dieta/análise
Suplementos Nutricionais
Enzimas
Alcaloides de Claviceps/sangue
Alcaloides de Claviceps/farmacocinética
Hidrólise
Fígado/efeitos dos fármacos
Fígado/metabolismo
Fígado/patologia
Masculino
Glândulas Mamárias Animais/metabolismo
Carne/análise
Nível de Efeito Adverso não Observado
Polissacarídeos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Dietary Fats); 0 (Enzymes); 0 (Ergot Alkaloids); 0 (Polysaccharides); EC 2.6.1.1 (Aspartate Aminotransferases); EC 2.6.1.2 (Alanine Transaminase)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170329
[St] Status:MEDLINE


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[PMID]:28257512
[Au] Autor:Comte A; Gräfenhan T; Links MG; Hemmingsen SM; Dumonceaux TJ
[Ad] Endereço:Agriculture and Agri-Food Canada Saskatoon Research and Development Centre, Saskatoon, Saskatchewan, Canada.
[Ti] Título:Quantitative molecular diagnostic assays of grain washes for Claviceps purpurea are correlated with visual determinations of ergot contamination.
[So] Source:PLoS One;12(3):e0173495, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We examined the epiphytic microbiome of cereal grain using the universal barcode chaperonin-60 (cpn60). Microbial community profiling of seed washes containing DNA extracts prepared from field-grown cereal grain detected sequences from a fungus identified only to Class Sordariomycetes. To identify the fungal sequence and to improve the reference database, we determined cpn60 sequences from field-collected and reference strains of the ergot fungus, Claviceps purpurea. These data allowed us to identify this fungal sequence as deriving from C. purpurea, and suggested that C. purpurea DNA is readily detectable on agricultural commodities, including those for which ergot was not identified as a grading factor. To get a sense of the prevalence and level of C. purpurea DNA in cereal grains, we developed a quantitative PCR assay based on the fungal internal transcribed spacer (ITS) and applied it to 137 samples from the 2014 crop year. The amount of Claviceps DNA quantified correlated strongly with the proportion of ergot sclerotia identified in each grain lot, although there was evidence that non-target organisms were responsible for some false positives with the ITS-based assay. We therefore developed a cpn60-targeted loop-mediated isothermal amplification assay and applied it to the same grain wash samples. The time to positive displayed a significant, inverse correlation to ergot levels determined by visual ratings. These results indicate that both laboratory-based and field-adaptable molecular diagnostic assays can be used to detect and quantify pathogen load in bulk commodities using cereal grain washes.
[Mh] Termos MeSH primário: Chaperoninas/genética
Claviceps/isolamento & purificação
Grãos Comestíveis/microbiologia
[Mh] Termos MeSH secundário: Claviceps/classificação
Claviceps/patogenicidade
Código de Barras de DNA Taxonômico
Grãos Comestíveis/genética
Sementes/genética
Sementes/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 3.6.1.- (Chaperonins)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170829
[Lr] Data última revisão:
170829
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170304
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0173495


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[PMID]:27765252
[Au] Autor:Shimshoni JA; Cuneah O; Sulyok M; Krska R; Sionov E; Barel S; Meller Harel Y
[Ad] Endereço:Kimron Veterinary Institute, Department of Toxicology, Bet Dagan 50250, Israel. Electronic address: jakobshimshoni@gmail.com.
[Ti] Título:Newly discovered ergot alkaloids in Sorghum ergot Claviceps africana occurring for the first time in Israel.
[So] Source:Food Chem;219:459-467, 2017 Mar 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Sorghum ergot is a disease caused commonly by C. africana. In 2015, ergot was identified for the first time in sorghum fields in Israel, leading to measures of eradication and quarantine. The aims of the study were to identify the ergot species by molecular and ergot alkaloid profile analysis, to determine the ergot alkaloid profile in pure honeydew and in infected sorghum silages and to estimate the safety of sorghum silages as a feed source. C. africana was rapidly and reliably identified by microscopical and molecular analysis. Dihydroergosine was identified as the major ergot alkaloid. Dihydrolysergol and dihydroergotamine were identified for the first time as significant ergot alkaloid components within the C. africana sclerotia, thereby providing for the first time a proof for the natural occurrence of dihydroergotamine. The sorghum silages were found to be safe for feed consumption, since the ergot alkaloids and the regulated mycotoxins were below their regulated limits.
[Mh] Termos MeSH primário: Claviceps/química
Alcaloides de Claviceps/análise
Doenças das Plantas/microbiologia
Sorghum/microbiologia
[Mh] Termos MeSH secundário: Grãos Comestíveis/microbiologia
Ergotaminas/análise
Israel
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ergot Alkaloids); 0 (Ergotamines); 0 (dihydroergosine)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170107
[Lr] Data última revisão:
170107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161022
[St] Status:MEDLINE


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[PMID]:27521625
[Au] Autor:van der Linde EJ; Pesicová K; Pazoutová S; Stodulková E; Flieger M; Kolarík M
[Ad] Endereço:Biosystematics Division, Plant Protection Research Institute, Agricultural Research Council, Private Bag X134, Pretoria 0121, South Africa. Electronic address: VDLindeE@arc.agric.za.
[Ti] Título:Ergot species of the Claviceps purpurea group from South Africa.
[So] Source:Fungal Biol;120(8):917-930, 2016 08.
[Is] ISSN:1878-6146
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Results of a survey and study of the Claviceps purpurea group of species in South Africa are being presented and five new species are described. Morphological descriptions are based on the anamorphs and four nuclear genetic loci. Claviceps fimbristylidis sp. nov. on Fimbristylis complanata was discovered wide-spread across five provinces of the country associated with water and represents the fourth Claviceps species recorded from the Cyperaceae. Claviceps monticola sp. nov. is described from Brachypodium flexum growing in mountain forests in Mpumalanga Province, as well as the northern Drakensberg southwards into the Eastern Cape Province. Claviceps pazoutovae sp. nov. is recorded from Stipa dregeana var. dregeana and Ehrharta erecta var. erecta, also associated with these mountain ranges. Claviceps macroura sp. nov. is recorded from Cenchrus macrourus from the Eastern Cape and Claviceps capensis sp. nov. from Ehrharta villosa var. villosa is recorded from the Western Cape Province. Claviceps cyperi, only recorded from South Africa is included in the study. Ergot alkaloid profiles of all species are provided and showed similarity to C. purpurea. Only C. cyperi and in lesser degree C. capensis, C. macroura, and C. pazoutovae produced ergot alkaloids in clinically significant amounts. Several reported species infect invasive grass species, native to South Africa, and thus represent potentially invasive species.
[Mh] Termos MeSH primário: Claviceps/classificação
Claviceps/isolamento & purificação
Microbiologia Ambiental
Alcaloides de Claviceps/análise
[Mh] Termos MeSH secundário: Cromatografia Líquida de Alta Pressão
Claviceps/química
Claviceps/genética
Análise por Conglomerados
DNA Fúngico/química
DNA Fúngico/genética
DNA Ribossômico/química
DNA Ribossômico/genética
Florestas
Componente 7 do Complexo de Manutenção de Minicromossomo/genética
Fator 1 de Elongação de Peptídeos/genética
Filogenia
Análise de Sequência de DNA
África do Sul
Tubulina (Proteína)/genética
Água
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Fungal); 0 (DNA, Ribosomal); 0 (Ergot Alkaloids); 0 (Peptide Elongation Factor 1); 0 (Tubulin); 059QF0KO0R (Water); EC 3.6.4.12 (Minichromosome Maintenance Complex Component 7)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171126
[Lr] Data última revisão:
171126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160814
[St] Status:MEDLINE


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[PMID]:27495979
[Au] Autor:Aboling S; Drotleff AM; Cappai MG; Kamphues J
[Ad] Endereço:Institute for Animal Nutrition, University of Veterinary Medicine, Hannover, Foundation, Bischofsholer Damm, 15, 30173, Hannover, Germany. sabine.aboling@tiho-hannover.de.
[Ti] Título:Contamination with ergot bodies (Claviceps purpurea sensu lato) of two horse pastures in Northern Germany.
[So] Source:Mycotoxin Res;32(4):207-219, 2016 Nov.
[Is] ISSN:1867-1632
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Because the occurrence of Claviceps in European pastures may have been overlooked to cause serious health problem for grazing animals, we documented the degree of Claviceps contamination in two horse pastures and estimated whether the horses could have ingested a critical quantity of alkaloids. We counted the Claviceps sclerotia and determined alkaloid levels using high performance liquid chromatography with fluorescence detection. Depending on the location, the number of sclerotia varied from 0.09 to 0.19 per square meter (central area) and from 0.23 to 55.8 per square meter (border strips). Alkaloid levels in individual sclerotia also varied in different genera of grasses, ranging from 0.98 ± 0.17 µg/kg in Agrostis sp. to 25.82 ± 9.73 µg/kg in Dactylis sp., equivalent to 0.98 µg/kg and 7.26 mg/kg. Sclerotia from Dactylis contained high levels of ergosine (0.209 % ± 0.100 %) and ergocristine (0.374 % ± 0.070 %). Depending on the localization in pastures, alkaloid levels in forage (dry matter, DM) ranged from 16.1 to 45.4 µg/kg in central areas and from 23.9 to 722 µg/kg in border strips. The amount of alkaloids that a horse could have ingested depended on its daily DM uptake, which was higher in the central areas (5.85 kg/day) than in the border strips (2.73 or 0.78 kg/day). In the central areas, this amount of alkaloids ranged from 94.2 to 265.9 µg/day; and in the border strips, from 65.3 (in 2.73 kg DM/day) to as much as 563.8 µg/day (in 0.78 kg DM/day). All these amounts are higher than the European averages for alkaloids ingested by horses via feedstuffs.
[Mh] Termos MeSH primário: Claviceps/química
Claviceps/isolamento & purificação
Contagem de Colônia Microbiana
Alcaloides de Claviceps/análise
Poaceae/microbiologia
[Mh] Termos MeSH secundário: Animais
Cromatografia Líquida de Alta Pressão
Fluorometria
Alemanha
Cavalos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ergot Alkaloids)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171110
[Lr] Data última revisão:
171110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160807
[St] Status:MEDLINE


  7 / 350 MEDLINE  
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[PMID]:27455930
[Au] Autor:Sivagnanam K; Komatsu E; Patrick S; Rampitsch C; Perreault H; Gräfenhan T
[Ti] Título:Rapid Screening of Ergot Alkaloids in Sclerotia by MALDI-TOF Mass Spectrometry.
[So] Source:J AOAC Int;99(4):895-8, 2016 Jul.
[Is] ISSN:1060-3271
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Ergot is a common disease of wheat and other cereal grains that is predominantly caused by Claviceps purpurea in the field, often affecting crop yield in addition to the environment. Infected grain can be contaminated with dark sclerotia, which contain fungal metabolites such as ergot alkaloids. The occurrence of ergot alkaloids in cereal grain is a major health concern for humans and livestock. Effective and rapid screening of these mycotoxins is crucial for producers, processors, and consumers of cereal-based food and feed grain. Established methods of ergot alkaloid screening based on LC-MS or GC-MS require laborious processes. A novel method using matrix-assisted laser desorption ionization (MALDI)-time-of-flight (TOF) MS was developed to identify four ergot alkaloids. Using dihydroxybenzoic acid as the matrix, ergosine, ergocornine, ergocryptine, and ergocristine were readily detected in individual sclerotia of C. purpurea. The accuracy of the identified ergot alkaloids was further confirmed by tandem MS analysis. MALDI-TOF MS is suitable for high-throughput screening of ergot alkaloids because it permits rapid and accurate identification, simple sample preparation, and no derivatization or chromatographic separation.
[Mh] Termos MeSH primário: Claviceps/química
Alcaloides de Claviceps/análise
[Mh] Termos MeSH secundário: Ergolinas/análise
Ergotaminas/análise
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos
Espectrometria de Massas em Tandem/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ergolines); 0 (Ergot Alkaloids); 0 (Ergotamines); 3E58HO2T0U (ergocristine); 511-09-1 (ergocryptine); 6WF908487H (ergosine); 7W36B25464 (ergocornine)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160727
[St] Status:MEDLINE
[do] DOI:10.5740/jaoacint.16-0117


  8 / 350 MEDLINE  
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[PMID]:27390873
[Au] Autor:Dopstadt J; Neubauer L; Tudzynski P; Humpf HU
[Ad] Endereço:Institute of Food Chemistry, Westfälische Wilhelms-Universität Münster, Corrensstraße 45, 48149 Münster, Germany.
[Ti] Título:The Epipolythiodiketopiperazine Gene Cluster in Claviceps purpurea: Dysfunctional Cytochrome P450 Enzyme Prevents Formation of the Previously Unknown Clapurines.
[So] Source:PLoS One;11(7):e0158945, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Claviceps purpurea is an important food contaminant and well known for the production of the toxic ergot alkaloids. Apart from that, little is known about its secondary metabolism and not all toxic substances going along with the food contamination with Claviceps are known yet. We explored the metabolite profile of a gene cluster in C. purpurea with a high homology to gene clusters, which are responsible for the formation of epipolythiodiketopiperazine (ETP) toxins in other fungi. By overexpressing the transcription factor, we were able to activate the cluster in the standard C. purpurea strain 20.1. Although all necessary genes for the formation of the characteristic disulfide bridge were expressed in the overexpression mutants, the fungus did not produce any ETPs. Isolation of pathway intermediates showed that the common biosynthetic pathway stops after the first steps. Our results demonstrate that hydroxylation of the diketopiperazine backbone is the critical step during the ETP biosynthesis. Due to a dysfunctional enzyme, the fungus is not able to produce toxic ETPs. Instead, the pathway end-products are new unusual metabolites with a unique nitrogen-sulfur bond. By heterologous expression of the Leptosphaeria maculans cytochrome P450 encoding gene sirC, we were able to identify the end-products of the ETP cluster in C. purpurea. The thioclapurines are so far unknown ETPs, which might contribute to the toxicity of other C. purpurea strains with a potentially intact ETP cluster.
[Mh] Termos MeSH primário: Claviceps
Sistema Enzimático do Citocromo P-450
Proteínas Fúngicas
Genes Fúngicos/fisiologia
Família Multigênica/fisiologia
Piperazinas
[Mh] Termos MeSH secundário: Claviceps/enzimologia
Claviceps/genética
Sistema Enzimático do Citocromo P-450/genética
Sistema Enzimático do Citocromo P-450/metabolismo
Proteínas Fúngicas/genética
Proteínas Fúngicas/metabolismo
Purinas/biossíntese
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungal Proteins); 0 (Piperazines); 0 (Purines); 0 (epipolythiodiketopiperazine); 9035-51-2 (Cytochrome P-450 Enzyme System)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170720
[Lr] Data última revisão:
170720
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160709
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0158945


  9 / 350 MEDLINE  
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[PMID]:26827914
[Au] Autor:Majeská Cudejková M; Vojta P; Valík J; Galuszka P
[Ad] Endereço:Department of Molecular Biology, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University in Olomouc, Slechtitelu 27, 783 71 Olomouc, Czech Republic. Electronic address: maria.cudejkova@upol.cz.
[Ti] Título:Quantitative and qualitative transcriptome analysis of four industrial strains of Claviceps purpurea with respect to ergot alkaloid production.
[So] Source:N Biotechnol;33(5 Pt B):743-754, 2016 Sep 25.
[Is] ISSN:1876-4347
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The fungus Claviceps purpurea is a biotrophic phytopathogen widely used in the pharmaceutical industry for its ability to produce ergot alkaloids (EAs). The fungus attacks unfertilized ovaries of grasses and forms sclerotia, which represent the only type of tissue where the synthesis of EAs occurs. The biosynthetic pathway of EAs has been extensively studied; however, little is known concerning its regulation. Here, we present the quantitative transcriptome analysis of the sclerotial and mycelial tissues providing a comprehensive view of transcriptional differences between the tissues that produce EAs and those that do not produce EAs and the pathogenic and non-pathogenic lifestyle. The results indicate metabolic changes coupled with sclerotial differentiation, which are likely needed as initiation factors for EA biosynthesis. One of the promising factors seems to be oxidative stress. Here, we focus on the identification of putative transcription factors and regulators involved in sclerotial differentiation, which might be involved in EA biosynthesis. To shed more light on the regulation of EA composition, whole transcriptome analysis of four industrial strains differing in their alkaloid spectra was performed. The results support the hypothesis proposing the composition of the amino acid pool in sclerotia to be an important factor regulating the final structure of the ergopeptines produced by Claviceps purpurea.
[Mh] Termos MeSH primário: Claviceps/genética
Claviceps/metabolismo
Alcaloides de Claviceps/biossíntese
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Biotecnologia
Proteínas Fúngicas/genética
Proteínas Fúngicas/metabolismo
Expressão Gênica
Perfilação da Expressão Gênica
Genes Fúngicos
Microbiologia Industrial
Estresse Oxidativo
Peptídeo Sintases/genética
Peptídeo Sintases/metabolismo
Polimorfismo de Nucleotídeo Único
Homologia de Sequência de Aminoácidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ergot Alkaloids); 0 (Fungal Proteins); EC 6.3.2.- (Peptide Synthases); EC 6.3.2.- (non-ribosomal peptide synthase)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170813
[Lr] Data última revisão:
170813
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160202
[St] Status:MEDLINE


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[PMID]:26693687
[Au] Autor:Roberts A; Beaumont C; Manzarpour A; Mantle P
[Ad] Endereço:Drug Metabolism and Pharmacokinetics, GlaxoSmithKline, Ware, Herts, SG12 0DP, UK.
[Ti] Título:Purpurolic acid: A new natural alkaloid from Claviceps purpurea (Fr.) Tul.
[So] Source:Fungal Biol;120(1):104-10, 2016 Jan.
[Is] ISSN:1878-6146
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A novel secondary metabolite from the sclerotia of Claviceps purpurea (Fr.) Tul. is described; the structure is based on (1)H and (13)C NMR spectroscopy and electrospray mass spectrometry. It has an elemental composition C10H16N2O7 and is comprised mainly of proline and alanine moieties, although without peptide linkage. Notably, these amino-acids are also components of the cyclic tripeptide side chain of several classic ergoline alkaloids. Designated as purpurolic acid, the new compound is the principal free amino-acid in ergot and its natural abundance exceeds that of the ergoline alkaloids with which it accumulates in parallel during parasitic development. In contrast, it does not accumulate in the fungus in axenic culture, even when ergotamine is synthesised. The extent to which the compound is a metabolite of other ergot fungi worldwide is unknown. Biological activity and metabolic significance also remain unknown, but purpurolic acid could become a biomarker for detection of ergot contamination in agricultural products of temperate latitudes.
[Mh] Termos MeSH primário: Alcaloides/química
Claviceps/metabolismo
[Mh] Termos MeSH secundário: Alcaloides/metabolismo
Claviceps/química
Espectroscopia de Ressonância Magnética
Espectrometria de Massas
Estrutura Molecular
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Alkaloids)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151224
[St] Status:MEDLINE



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