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[PMID]:28916922
[Au] Autor:Li Y; Tong L; Deng L; Liu Q; Xing Y; Wang C; Liu B; Yang X; Xu M
[Ad] Endereço:National Maize Improvement Center of China, China Agricultural University, Beijing, 100193, People's Republic of China.
[Ti] Título:Evaluation of ZmCCT haplotypes for genetic improvement of maize hybrids.
[So] Source:Theor Appl Genet;130(12):2587-2600, 2017 Dec.
[Is] ISSN:1432-2242
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:KEY MESSAGE: The elite ZmCCT haplotypes which have no transposable element in the promoter could enhance maize resistance to Gibberella stalk rot and improve yield-related traits, while having no or mild impact on flowering time. Therefore, they are expected to have great value in future maize breeding programs. A CCT domain-containing gene, ZmCCT, is involved in both photoperiod response and stalk rot resistance in maize. At least 15 haplotypes are present at the ZmCCT locus in maize germplasm, whereas only three of them are found in Chinese commercial maize hybrids. Here, we evaluated ZmCCT haplotypes for their potential application in corn breeding. Nine resistant ZmCCT haplotypes that have no CACTA-like transposable element in the promoter were introduced into seven elite maize inbred lines by marker-assisted backcrossing. The resultant 63 converted lines had 0.7-5.1 Mb of resistant ZmCCT donor segments with over 90% recovery rates. All converted lines tested exhibited enhanced resistance to maize stalk rot but varied in photoperiod sensitivity. There was a close correlation between the hybrids and their parental lines with respect to both resistance performance and photoperiod sensitivity. Furthermore, in a given hybrid A5302/83B28, resistant ZmCCT haplotype could largely improve yield-related traits, such as ear length and 100-kernel weight, resulting in enhanced grain yield. Of nine resistant ZmCCT haplotypes, haplotype H5 exhibited excellent performance for both flowering time and stalk rot resistance and is thus expected to have potential value in future maize breeding programs.
[Mh] Termos MeSH primário: Hibridização Genética
Fotoperíodo
Melhoramento Vegetal
Proteínas de Plantas/genética
Zea mays/genética
[Mh] Termos MeSH secundário: Elementos de DNA Transponíveis
Resistência à Doença/genética
Flores/fisiologia
Gibberella
Haplótipos
Fenótipo
Regiões Promotoras Genéticas
Zea mays/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Transposable Elements); 0 (Plant Proteins)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170917
[St] Status:MEDLINE
[do] DOI:10.1007/s00122-017-2978-1


  2 / 330 MEDLINE  
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[PMID]:28880897
[Au] Autor:Tian H; Wang H; Hui X; Wang Z; Drijber RA; Liu J
[Ad] Endereço:Key Laboratory of Plant Nutrition and Agro-environment in Northwest China, Ministry of Agriculture/College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi, China.
[Ti] Título:Changes in soil microbial communities after 10 years of winter wheat cultivation versus fallow in an organic-poor soil in the Loess Plateau of China.
[So] Source:PLoS One;12(9):e0184223, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Agricultural management methods, such as cultivation or fallowing, have led to significant changes in soil fertility and hence, crop yield. Such changes may have stemmed from changes in soil microbial communities and associated biogeochemical processes. This phenomenon is particularly true in organic-poor soil in the Loess Plateau of China. In this study, we examined three existing soil management regimes as part of a 10-year field experiment and evaluated their effects on fungal and bacterial community structures by performing high-throughput 454 pyrosequencing. These management regimes were (i) fertilized winter wheat (Triticum aestivum L.) (FW), (ii) continuous natural fallow with weeds but without crop grown (NF), and (iii) continuous bare fallow without weeds or crop grown (BF). After 10 years, soil organic carbon (SOC), microbial biomass carbon (MBC), and available potassium (K) concentrations were highest in NF. Soil N behaved differently, with BF obtaining the highest nitrate nitrogen (N). Meanwhile, slight differences in total N (TN) were observed among FW, NF, and BF. Available phosphorus (P) was highest and available K was lowest in FW. Microbial communities were dominated by Ascomycota (59.1% of fungal sequences), and Acidobacteria, Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria (75.7% of bacterial sequences) in FW, NF and BF at the phylum level. Soil management regimes did not affect the fungal and bacterial richness and diversity but significantly modified their community compositions. Compared with FW, the abundances of Ascomycota (fungi phylum) and Alternaria, Gibberella, and Emericella (fungi genus) were increased by NF, whereas the values of Chaetomium, Humicola, and Cryptococcus (fungi genus) were decreased by BF. The abundances of Verrucomicrobia (bacteria phylum), and Steroidobacter (bacteria genus) were increased by NF, and Bacteroides (bacteria genus) was increased by BF. Canonical correspondence analysis showed that SOC, available P, and TN might be the key factors in community formation. Therefore, the decadal absence of plants (BF) affected soil fertility by increased available K and nitrate N, whileas natural fallow (NF) affected soil fertility by increased SOC, available K, and MBC, and they all changed fungal and bacterial community compositions.
[Mh] Termos MeSH primário: Microbiologia do Solo
Solo/classificação
Triticum
[Mh] Termos MeSH secundário: Alternaria/isolamento & purificação
Ascomicetos/isolamento & purificação
Biomassa
Carbono
Chaetomium/isolamento & purificação
China
Cryptococcus/isolamento & purificação
Emericella/isolamento & purificação
Gibberella/isolamento & purificação
Nitrogênio/análise
Potássio/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Soil); 7440-44-0 (Carbon); N762921K75 (Nitrogen); RWP5GA015D (Potassium)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170908
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184223


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[PMID]:28555262
[Au] Autor:Ma C; Ma X; Yao L; Liu Y; Du F; Yang X; Xu M
[Ad] Endereço:National Maize Improvement Centre of China, China Agricultural University, Beijing, People's Republic of China.
[Ti] Título:qRfg3, a novel quantitative resistance locus against Gibberella stalk rot in maize.
[So] Source:Theor Appl Genet;130(8):1723-1734, 2017 Aug.
[Is] ISSN:1432-2242
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:KEY MESSAGE: A quantitative trait locus  qRfg3 imparts recessive resistance to maize Gibberella stalk rot. qRfg3 has been mapped into a 350-kb interval and could reduce the disease severity index by ~26.6%. Gibberella stalk rot, caused by the fungal pathogen Fusarium graminearum, severely affects maize yield and grain quality worldwide. To identify more resistance quantitative trait loci (QTLs) against this disease, we analyzed a recombinant inbred line (RIL) population derived from a cross between resistant H127R and susceptible C7-2 inbred lines. Within this population, maize resistance to Gibberella stalk rot had high broad-sense heritability. A major QTL, qRfg3, on chromosome 3 was consistently detected across three field trials, accounting for 10.7-19.4% of the total phenotypic variation. Using a progeny-based sequential fine-mapping strategy, we narrowed qRfg3 down to an interval of ~350 kb. We further demonstrated that qRfg3 is a recessive resistance locus to Gibberella stalk rot that reduced the disease severity index by ~26.6%. Both the gene location and recessive genetic mode distinguish qRfg3 from other stalk rot resistance loci. Hence, qRfg3 is valuable as a complement to existing resistance QTLs to improve maize resistance to Gibberella stalk rot.
[Mh] Termos MeSH primário: Resistência à Doença/genética
Gibberella
Doenças das Plantas/genética
Locos de Características Quantitativas
Zea mays/genética
[Mh] Termos MeSH secundário: Mapeamento Cromossômico
Cruzamentos Genéticos
Ligação Genética
Marcadores Genéticos
Genótipo
Fenótipo
Doenças das Plantas/microbiologia
Zea mays/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Genetic Markers)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170531
[St] Status:MEDLINE
[do] DOI:10.1007/s00122-017-2921-5


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[PMID]:27709251
[Au] Autor:Brauner PC; Melchinger AE; Schrag TA; Utz HF; Schipprack W; Kessel B; Ouzunova M; Miedaner T
[Ad] Endereço:Institute of Plant Breeding, Seed Science and Population Genetics (350a), University of Hohenheim, 70593, Stuttgart, Germany.
[Ti] Título:Low validation rate of quantitative trait loci for Gibberella ear rot resistance in European maize.
[So] Source:Theor Appl Genet;130(1):175-186, 2017 Jan.
[Is] ISSN:1432-2242
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:KEY MESSAGE: Six quantitative trait loci (QTL) for Gibberella ear rot resistance in maize were tested in two different genetic backgrounds; three QTL displayed an effect in few near isogenic line pairs. Few quantitative trait loci (QTL) mapping studies for Gibberella ear rot (GER) have been conducted, but no QTL have been verified so far. QTL validation is prudent before their implementation into marker-assisted selection (MAS) programs. Our objectives were to (1) validate six QTL for GER resistance, (2) evaluate the QTL across two genetic backgrounds, (3) investigate the genetic background outside the targeted introgressions. Pairs of near isogenic lines (NILs) segregating for a single QTL (Qger1, Qger2, Qger10, Qger13, Qger16, or Qger21) were developed by recurrent backcross until generation BC S . Donor parents (DP) carrying QTL were backcrossed to a susceptible (UH009) and a moderately resistant (UH007) recurrent parent. MAS was performed using five SNP markers covering a region of 40 cM around each QTL. All NILs were genotyped with the MaizeSNP50 assay and phenotyped for GER severity and deoxynivalenol and zearalenone content. Traits were significantly (P < 0.001) intercorrelated. Out of 34 NIL pairs with the UH009 genetic background, three pairs showed significant differences in at least one trait for three QTL (Qger1, Qger2, Qger13). Out of 25 NIL pairs with the UH007 genetic background, five pairs showed significant differences in at least one trait for two QTL (Qger2, Qger21). However, Qger16, Qger10 and Qger13 were most likely false positives. The genetic background possibly affected NIL pairs comparisons due to linkage drag and/or epistasis with residual loci from the DP in non-target regions. In conclusion, validation rates were disappointingly low, which further indicates that GER resistance is controlled by many low-effect QTL.
[Mh] Termos MeSH primário: Resistência à Doença/genética
Gibberella
Doenças das Plantas/genética
Locos de Características Quantitativas
Zea mays/genética
[Mh] Termos MeSH secundário: Mapeamento Cromossômico
Cruzamentos Genéticos
Ligação Genética
Genótipo
Fenótipo
Melhoramento Vegetal
Doenças das Plantas/microbiologia
Polimorfismo de Nucleotídeo Único
Tricotecenos/análise
Zea mays/microbiologia
Zearalenona/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Trichothecenes); 5W827M159J (Zearalenone); JT37HYP23V (deoxynivalenol)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170917
[Lr] Data última revisão:
170917
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161007
[St] Status:MEDLINE
[do] DOI:10.1007/s00122-016-2802-3


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[PMID]:27158946
[Au] Autor:Parker NS; Anderson NR; Richmond DS; Long EY; Wise KA; Krupke CH
[Ad] Endereço:Department of Entomology, Purdue University, West Lafayette, IN, USA.
[Ti] Título:Larval western bean cutworm feeding damage encourages the development of Gibberella ear rot on field corn.
[So] Source:Pest Manag Sci;73(3):546-553, 2017 Mar.
[Is] ISSN:1526-4998
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: A 2 year study was conducted to determine whether western bean cutworm (Striacosta albicosta Smith) (WBC) larval feeding damage increases severity of the fungal disease Gibberella ear rot [Fusarium graminearum (Schwein.) Petch] in field corn (Zea mays L.). The effect of a quinone-outside inhibiting fungicide, pyraclostrobin, on Gibberella ear rot severity and mycotoxin production, both with and without WBC pressure, was also evaluated. The impact of each variable was assessed individually and in combination to determine the effect of each upon ear disease severity. RESULTS: There was a positive correlation between the presence of WBC larvae in field corn and Gibberella ear rot severity under inoculated conditions in the 2 years of the experiment. An application of pyraclostrobin did not impact Gibberella ear rot development when applied at corn growth stage R1 (silks first emerging). CONCLUSION: Feeding damage from WBC larvae significantly increases the development of F. graminearum in field corn. We conclude that an effective integrated management strategy for Gibberella ear rot should target the insect pest first, in an effort to limit disease severity and subsequent mycotoxin production by F. graminearum in kernels. © 2016 Society of Chemical Industry.
[Mh] Termos MeSH primário: Gibberella/fisiologia
Mariposas/fisiologia
Doenças das Plantas/microbiologia
Zea mays/microbiologia
[Mh] Termos MeSH secundário: Animais
Comportamento Alimentar
Gibberella/crescimento & desenvolvimento
Indiana
Larva/fisiologia
Mariposas/crescimento & desenvolvimento
Zea mays/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170407
[Lr] Data última revisão:
170407
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160510
[St] Status:MEDLINE
[do] DOI:10.1002/ps.4313


  6 / 330 MEDLINE  
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[PMID]:28262122
[Au] Autor:Wu YQ; Cao Y; Liu X; Cheng ZH
[Ad] Endereço:Department of Pharmacognosy, School of Pharmacy, Fudan University, Shanghai 201203, China.
[Ti] Título:Regio- and stereo-selective hydroxylations of ingenane diterpenoids by Mortierella ramanniana and Gibberella fujikuroi.
[So] Source:Chin J Nat Med;14(12):939-945, 2016 Dec.
[Is] ISSN:1875-5364
[Cp] País de publicação:China
[La] Idioma:eng
[Ab] Resumo:The regio- and stereo-selective hydroxylations of two ingenane diterpenoids, 20-deoxyingenol (1) and 13-oxyingenol dodecanoat (2), by the filamentous fungi Mortierella ramanniana and Gibberella fujikuroi were investigated in the present study. Four undescribed metabolites (3-6) of substrate 1 and two undescribed metabolites (7 and 8) of substrate 2 were isolated. All the metabolites were identified as hydroxylated ingenane derivatives by extensive NMR and HR-ESI-MS data analyses. All the biotransformed compounds and the substrates were evaluated for their cytotoxicities against three human cancer cell lines, including human colon cancer Caco-2, breast cancer MCF-7, and adriamycin (ADM)-resistant MCF-7/ADM cell lines. All ingenane alcohols (1, and 3-6) displayed no significant cytotoxic activities. The substrate 13-oxyingenol dodecanoat (2) showed moderate cytotoxicity with IC values being 35.59 ± 5.37 µmol·L (Caco-2), 24.04 ± 4.70 µmol·L (MCF-7), and 22.24 ± 5.19 µmol·L (MCF-7/ADM). However, metabolites 7 and 8 displayed no significant cytotoxicity. These results indicated that the hydroxylation at the C-13 aliphatic acid ester of substrate 2 can significantly reduce the cytotoxic activity.
[Mh] Termos MeSH primário: Diterpenos/química
Diterpenos/metabolismo
Gibberella/metabolismo
Mortierella/metabolismo
[Mh] Termos MeSH secundário: Antineoplásicos/química
Antineoplásicos/metabolismo
Biotransformação
Linhagem Celular Tumoral
Seres Humanos
Hidroxilação
Estrutura Molecular
Estereoisomerismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Diterpenes); 0 (ingenane)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170320
[Lr] Data última revisão:
170320
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170307
[St] Status:MEDLINE


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[PMID]:27646209
[Au] Autor:Rios-Iribe EY; Hernández-Calderón OM; Escamilla-Silva EM
[Ad] Endereço:Programa Regional de Posgrado en Biotecnología, Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Sinaloa, Av. de las Américas y Blvd. Universitarios, Ciudad Universitaria, 80013, Culiacán, Sinaloa, Mexico.
[Ti] Título:Kinetic analysis of the uptake of glucose and corn oil used as carbon sources in batch cultures of Gibberella fujikuroi.
[So] Source:World J Microbiol Biotechnol;32(11):182, 2016 Nov.
[Is] ISSN:1573-0972
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:This study determined the specific uptake rate of glucose and corn oil substrates used as carbon sources in batch cultures of Gibberella fujikuroi. We tested three biological models of growth rate: Monod, logistic and lag-exponential. With respect to the substrate consumption rate, we tested two models: constant cell yield (CCY) and law of mass action (LMA). The experimental data obtained from the culture with glucose as substrate correlated satisfactorily with the logistic/LMA model, indicating that the cell yield was variable. In the case of corn oil as carbon source, considering total residual lipids as substrate in the culture broth, the model with the best correlation was the lag-exp/CCY model. The quantification by GC of the three main fatty acids (linoleic, oleic and palmitic) in the culture medium showed a cumulative behavior, with a maximum concentration of each acid at 36 h. We established a more explicit mechanism of the consumption of corn oil, consisting of two stages: generation of fatty acids by hydrolysis and consumption by cellular uptake. The kinetic of hydrolysable lipids was of first order. We found that the hydrolysis rate of corn oil is not a limiting factor for the uptake of fatty acids by the microorganism. We also established, based on the analysis of the identical mathematical structure of consumption kinetics, that the uptake of fatty acids is faster than the uptake of glucose.
[Mh] Termos MeSH primário: Técnicas de Cultura Celular por Lotes/métodos
Óleo de Milho/metabolismo
Gibberella/crescimento & desenvolvimento
Glucose/metabolismo
[Mh] Termos MeSH secundário: Biomassa
Carbono/metabolismo
Meios de Cultura
Cinética
Lipídeos/química
Modelos Logísticos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Culture Media); 0 (Lipids); 7440-44-0 (Carbon); 8001-30-7 (Corn Oil); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160921
[St] Status:MEDLINE
[do] DOI:10.1007/s11274-016-2139-8


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[PMID]:27529072
[Au] Autor:Li Y; Liu S; Qin Y; Xing R; Chen X; Li K; Li P
[Ad] Endereço:Institute of Oceanology, Chinese Academy of Sciences, No. 7 Nanhai Road, Qingdao 266071, China; University of Chinese Academy of Sciences, Beijing 100049, China; Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, No. 7 Nanhai Road, Qingdao 266071, Ch
[Ti] Título:Synthesis of Novel Pyrimethanil Grafted Chitosan Derivatives with Enhanced Antifungal Activity.
[So] Source:Biomed Res Int;2016:8196960, 2016.
[Is] ISSN:2314-6141
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In this study, three pyrimethanil grafted chitosan (PML-g-CS) derivatives were obtained. The structures of the conjugates were confirmed by FT-IR, (1)H NMR, and EA. The grafting ratios were measured by HPLC. Antifungal properties of pyrimethanil grafted chitosan (PML-g-CS) derivatives against the plant pathogenic fungi Rhizoctonia solani and Gibberella zeae were investigated at concentrations of 100, 200, and 400 mg/L. The PML-g-CS derivatives showed enhanced antifungal activity in comparison with chitosan. The PML-g-CS-1 showed the best antifungal activity against R. solani, whose antifungal index was 58.32%. The PML-g-CS-2 showed the best antifungal activity against G. zeae, whose antifungal index was 53.48%. The conjugation of chitosan and pyrimethanil showed synergistic effect. The PML-g-CS derivatives we developed showed potential for further study and application in crop protection.
[Mh] Termos MeSH primário: Quitosana/química
Fungicidas Industriais/química
Pirimidinas/química
[Mh] Termos MeSH secundário: Bioensaio
Cromatografia Líquida de Alta Pressão
Cromatografia em Camada Delgada
Proteção de Cultivos
Produtos Agrícolas/microbiologia
Relação Dose-Resposta a Droga
Gibberella/efeitos dos fármacos
Espectroscopia de Ressonância Magnética
Testes de Sensibilidade Microbiana
Peso Molecular
Rhizoctonia/efeitos dos fármacos
Espectroscopia de Infravermelho com Transformada de Fourier
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungicides, Industrial); 0 (Pyrimidines); 6IA5HP6C8Z (pyrimethanil); 9012-76-4 (Chitosan)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170328
[Lr] Data última revisão:
170328
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160817
[St] Status:MEDLINE
[do] DOI:10.1155/2016/8196960


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[PMID]:27521799
[Au] Autor:Baydoun S; Wahab AT; Bano S; Imad R; Choudhary MI
[Ad] Endereço:University of Iowa Hospital and Clinics, Iowa City, IA 52242, United States.
[Ti] Título:Microbial-catalysed derivatization of anti-cancer drug exemestane and cytotoxicity of resulting metabolites against human breast adenocarcinoma cell line (MCF-7) in vitro.
[So] Source:Steroids;115:67-74, 2016 Nov.
[Is] ISSN:1878-5867
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Structural transformation of anticancer drug exemestane (1) with fungi Cunninghamella blakesleeana (ATCC 8688A), Curvularia lunata (ATCC 12017), Aspergillus niger (ATCC 10549), and Gibberella fujikuroi (ATCC 10704) yielded eleven metabolites 2-12, in which 2 and 8 were identified as new. Their structures were characterized as 6-methylene-5α-androstane-3ß,16ß,17ß-triol (2), 17ß-hydroxy-6-methyleneandrosta-4-ene-3-one (3), 6α-spiroxirandrost-4-ene-3,17-dione (4), 6-methyleneandrosta-4-ene-3,17-dione (5), 6ß,17ß-dihydroxyandrost-4-en-3-one (6), 17ß-hydroxy-6α-spiroxirandrost-1,4-diene-3-one (7), 17ß-hydroxy-6α-hydroxymethylandrosta-1,4-dien-3-one (8), 6α-hydroxymethylandrosta-1,4-diene-3,17-dione (9), 17ß-hydroxy-6-methyleneandrosta-1,4-diene-3,16-dione (10), 6α-hydroxy-4-androstene-3,17-dione (11), and 6α-hydroxymethylandrost-4-ene-3,17-dione (12). Substrate 1, and its transformed products were evaluated for their cytotoxicity against breast cancer cell line (MCF-7). Compound 3 was found to be moderately active with an IC of 33.43±4.01µM, in comparison to the standard anti-cancer drug, doxorubicin (IC =0.92±0.1µM).
[Mh] Termos MeSH primário: Androstadienos/metabolismo
Androstadienos/farmacologia
Antineoplásicos/metabolismo
Antineoplásicos/farmacologia
Neoplasias da Mama/metabolismo
[Mh] Termos MeSH secundário: Androstadienos/química
Antineoplásicos/química
Aspergillus niger/metabolismo
Sobrevivência Celular/efeitos dos fármacos
Cunninghamella/metabolismo
Fermentação
Gibberella/metabolismo
Seres Humanos
Células MCF-7
Estrutura Molecular
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Androstadienes); 0 (Antineoplastic Agents); NY22HMQ4BX (exemestane)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170830
[Lr] Data última revisão:
170830
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160814
[St] Status:MEDLINE


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[PMID]:27133903
[Au] Autor:Ahmad MS; Zafar S; Yousuf S; Wahab AT; Rahman AU; Choudhary MI
[Ad] Endereço:H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan.
[Ti] Título:Biotransformation of 6-dehydroprogesterone with Aspergillus niger and Gibberella fujikuroi.
[So] Source:Steroids;112:62-7, 2016 Aug.
[Is] ISSN:1878-5867
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Microbial transformation of 6-dehydroprogesterone (1) with Aspergillus niger yielded three new metabolites, including 6ß-chloro-7α,11α-dihydroxypregna-4-ene-3,20-dione (2), 7α-chloro-6ß,11α-dihydroxypregna-4-ene-3,20-dione (3), and 6α,7α-epoxy-11α-hydroxypregna-4-ene-3,20-dione (4), and two known metabolites; 6α,7α-epoxypregna-4-ene-3,20-dione (5), and 11α-hydroxypregna-4,6-diene-3,20-dione (6). Compounds 2, and 3 contain chlorohydrin moiety at C-6, and C-7, respectively. The biotransformation of 1 with Gibberella fujikuroi yielded a known compound, 11α,17ß-dihydroxyandrosta-4,6-dien-3-one (7).
[Mh] Termos MeSH primário: Aspergillus niger/metabolismo
Gibberella/metabolismo
Progesterona/química
Progesterona/metabolismo
[Mh] Termos MeSH secundário: Biotransformação
Estrutura Molecular
Estereoisomerismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
4G7DS2Q64Y (Progesterone)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170705
[Lr] Data última revisão:
170705
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160503
[St] Status:MEDLINE



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