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  1 / 176 MEDLINE  
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[PMID]:28873985
[Au] Autor:Badino SF; Bathke JK; Sørensen TH; Windahl MS; Jensen K; Peters GHJ; Borch K; Westh P
[Ad] Endereço:Research Unit for Functional Biomaterials, Department of Science and Environment, INM, Roskilde University, 1 Universitetsvej, Build. 28 C, DK-4000, Roskilde, Denmark.
[Ti] Título:The influence of different linker modifications on the catalytic activity and cellulose affinity of cellobiohydrolase Cel7A from Hypocrea jecorina.
[So] Source:Protein Eng Des Sel;30(7):495-501, 2017 Jul 01.
[Is] ISSN:1741-0134
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Various cellulases consist of a catalytic domain connected to a carbohydrate-binding module (CBM) by a flexible linker peptide. The linker if often strongly O-glycosylated and typically has a length of 20-50 amino acid residues. Functional roles, other than connecting the two folded domains, of the linker and its glycans, have been widely discussed, but experimental evidence remains sparse. One of the most studied cellulose degrading enzymes is the multi-domain cellobiohydrolase Cel7A from Hypocrea jecorina. Here, we designed variants of Cel7A with mutations in the linker region to elucidate the role of the linker. We found that moderate modification of the linker could result in significant changes in substrate affinity and catalytic efficacy. These changes were quite different for different linker variants. Thus, deletion of six residues near the catalytic domain had essentially no effects on enzyme function. Conversely, a substitution of four glycosylation sites near the middle of the linker reduced substrate affinity and increased maximal turnover. The observation of weaker binding provides some support of recent suggestions that linker glycans may be directly involved in substrate interactions. However, a variant with several inserted glycosylation sites near the CBM also showed lower affinity for the substrate compared to the wild-type, and we suggest that substrate interactions of the glycans depend on their exact location as well as other factors such as changes in structure and dynamics of the linker peptide.
[Mh] Termos MeSH primário: Catálise
Celulose 1,4-beta-Celobiosidase/química
Hypocrea/enzimologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos/genética
Celulase/química
Celulose/química
Celulose 1,4-beta-Celobiosidase/genética
Cinética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9004-34-6 (Cellulose); EC 3.2.1.4 (Cellulase); EC 3.2.1.91 (Cellulose 1,4-beta-Cellobiosidase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE
[do] DOI:10.1093/protein/gzx036


  2 / 176 MEDLINE  
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[PMID]:28860192
[Au] Autor:Goedegebuur F; Dankmeyer L; Gualfetti P; Karkehabadi S; Hansson H; Jana S; Huynh V; Kelemen BR; Kruithof P; Larenas EA; Teunissen PJM; Ståhlberg J; Payne CM; Mitchinson C; Sandgren M
[Ad] Endereço:From DuPont Industrial Biosciences, Archimedesweg 30, Leiden 2333CN, The Netherlands, frits.goedegebuur@dupont.com.
[Ti] Título:Improving the thermal stability of cellobiohydrolase Cel7A from by directed evolution.
[So] Source:J Biol Chem;292(42):17418-17430, 2017 Oct 20.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Secreted mixtures of cellulases are able to efficiently degrade cellulosic biomass to fermentable sugars at large, commercially relevant scales. Cel7A, cellobiohydrolase I, from glycoside hydrolase family 7, is the workhorse enzyme of the process. However, the thermal stability of Cel7A limits its use to processes where temperatures are no higher than 50 °C. Enhanced thermal stability is desirable to enable the use of higher processing temperatures and to improve the economic feasibility of industrial biomass conversion. Here, we enhanced the thermal stability of Cel7A through directed evolution. Sites with increased thermal stability properties were combined, and a Cel7A variant (FCA398) was obtained, which exhibited a 10.4 °C increase in and a 44-fold greater half-life compared with the wild-type enzyme. This Cel7A variant contains 18 mutated sites and is active under application conditions up to at least 75 °C. The X-ray crystal structure of the catalytic domain was determined at 2.1 Å resolution and showed that the effects of the mutations are local and do not introduce major backbone conformational changes. Molecular dynamics simulations revealed that the catalytic domain of wild-type Cel7A and the FCA398 variant exhibit similar behavior at 300 K, whereas at elevated temperature (475 and 525 K), the FCA398 variant fluctuates less and maintains more native contacts over time. Combining the structural and dynamic investigations, rationales were developed for the stabilizing effect at many of the mutated sites.
[Mh] Termos MeSH primário: Celulose 1,4-beta-Celobiosidase
Proteínas Fúngicas
Temperatura Alta
Hypocrea
[Mh] Termos MeSH secundário: Celulose 1,4-beta-Celobiosidase/química
Celulose 1,4-beta-Celobiosidase/genética
Cristalografia por Raios X
Evolução Molecular Direcionada
Estabilidade Enzimática/genética
Proteínas Fúngicas/química
Proteínas Fúngicas/genética
Hypocrea/enzimologia
Hypocrea/genética
Simulação de Dinâmica Molecular
Domínios Proteicos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungal Proteins); EC 3.2.1.91 (Cellulose 1,4-beta-Cellobiosidase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171027
[Lr] Data última revisão:
171027
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170902
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M117.803270


  3 / 176 MEDLINE  
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[PMID]:28244592
[Au] Autor:Badino SF; Christensen SJ; Kari J; Windahl MS; Hvidt S; Borch K; Westh P
[Ad] Endereço:Research Unit for Functional Biomaterials, Department of Science and Environment, INM, Roskilde University, 1 Universitetsvej, Build. 28C, DK-4000, Roskilde, Denmark.
[Ti] Título:Exo-exo synergy between Cel6A and Cel7A from Hypocrea jecorina: Role of carbohydrate binding module and the endo-lytic character of the enzymes.
[So] Source:Biotechnol Bioeng;114(8):1639-1647, 2017 Aug.
[Is] ISSN:1097-0290
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Synergy between cellulolytic enzymes is essential in both natural and industrial breakdown of biomass. In addition to synergy between endo- and exo-lytic enzymes, a lesser known but equally conspicuous synergy occurs among exo-acting, processive cellobiohydrolases (CBHs) such as Cel7A and Cel6A from Hypocrea jecorina. We studied this system using microcrystalline cellulose as substrate and found a degree of synergy between 1.3 and 2.2 depending on the experimental conditions. Synergy between enzyme variants without the carbohydrate binding module (CBM) and its linker was strongly reduced compared to the wild types. One plausible interpretation of this is that exo-exo synergy depends on the targeting role of the CBM. Many earlier works have proposed that exo-exo synergy was caused by an auxiliary endo-lytic activity of Cel6A. However, biochemical data from different assays suggested that the endo-lytic activity of both Cel6A and Cel7A were 10 -10 times lower than the common endoglucanase, Cel7B, from the same organism. Moreover, the endo-lytic activity of Cel7A was 2-3-fold higher than for Cel6A, and we suggest that endo-like activity of Cel6A cannot be the main cause for the observed synergy. Rather, we suggest the exo-exo synergy found here depends on different specificities of the enzymes possibly governed by their CBMs. Biotechnol. Bioeng. 2017;114: 1639-1647. © 2017 Wiley Periodicals, Inc.
[Mh] Termos MeSH primário: Celulose/química
Proteínas Fúngicas/química
Hypocrea/enzimologia
[Mh] Termos MeSH secundário: Sítios de Ligação
Sinergismo Farmacológico
Ativação Enzimática
Complexos Multienzimáticos
Ligação Proteica
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungal Proteins); 0 (Multienzyme Complexes); 9004-34-6 (Cellulose); OP1R32D61U (microcrystalline cellulose)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170301
[St] Status:MEDLINE
[do] DOI:10.1002/bit.26276


  4 / 176 MEDLINE  
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[PMID]:28026938
[Au] Autor:Kari J; Kont R; Borch K; Buskov S; Olsen JP; Cruyz-Bagger N; Väljamäe P; Westh P
[Ad] Endereço:Research Unit for Functional Biomaterials, Roskilde University , Roskilde, Denmark.
[Ti] Título:Anomeric Selectivity and Product Profile of a Processive Cellulase.
[So] Source:Biochemistry;56(1):167-178, 2017 Jan 10.
[Is] ISSN:1520-4995
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cellobiohydrolases (CBHs) make up an important group of enzymes for both natural carbon cycling and industrial deconstruction of lignocellulosic biomass. The consecutive hydrolysis of one cellulose strand relies on an intricate pattern of enzyme-substrate interactions in the long, tunnel-shaped binding site of the CBH. In this work, we have investigated the initial complexation mode with cellulose of the most thoroughly studied CBH, Cel7A from Hypocrea jecorina (HjCel7A). We found that HjCel7A predominantly produces glucose when it initiates a processive run on insoluble microcrystalline cellulose, confirming the validity of an even and odd product ratio as an estimate of processivity. Moreover, the glucose released from cellulose was predominantly α-glucose. A link between the initial binding mode of the enzyme and the reducing end configuration was investigated by inhibition studies with the two anomers of cellobiose. A clear preference for ß-cellobiose in product binding site +2 was observed for HjCel7A, but not the homologous endoglucanase, HjCe7B. Possible relationships between this anomeric preference in the product site and the prevalence of odd-numbered initial-cut products are discussed, and a correlation between processivity and anomer selectivity is proposed.
[Mh] Termos MeSH primário: Celobiose/metabolismo
Celulose 1,4-beta-Celobiosidase/metabolismo
Proteínas Fúngicas/metabolismo
Hypocrea/enzimologia
[Mh] Termos MeSH secundário: Algoritmos
Técnicas Biossensoriais
Celobiose/química
Celulose/análogos & derivados
Celulose/química
Celulose/metabolismo
Celulose 1,4-beta-Celobiosidase/química
Cromatografia Líquida
Cristalografia por Raios X
Proteínas Fúngicas/química
Glucose/química
Glucose/metabolismo
Hypocrea/metabolismo
Isoenzimas/química
Isoenzimas/metabolismo
Cinética
Espectrometria de Massas
Modelos Moleculares
Estrutura Molecular
Ligação Proteica
Domínios Proteicos
Especificidade por Substrato
Tetroses/química
Tetroses/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungal Proteins); 0 (Isoenzymes); 0 (Tetroses); 16462-44-5 (Cellobiose); 38819-01-1 (cellotetraose); 9004-34-6 (Cellulose); EC 3.2.1.91 (Cellulose 1,4-beta-Cellobiosidase); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170522
[Lr] Data última revisão:
170522
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161228
[St] Status:MEDLINE
[do] DOI:10.1021/acs.biochem.6b00636


  5 / 176 MEDLINE  
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[PMID]:27617666
[Au] Autor:Olsen JP; Borch K; Westh P
[Ad] Endereço:Research Unit for Functional Biomaterials, Department of Science and Environment, Roskilde University, INM, 1 Universitetsvej, Build. 28, DK-4000, Roskilde, Denmark.
[Ti] Título:Endo/exo-synergism of cellulases increases with substrate conversion.
[So] Source:Biotechnol Bioeng;114(3):696-700, 2017 Mar.
[Is] ISSN:1097-0290
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Synergy between cellulolytic enzymes is important for their industrial utilization, and numerous studies have addressed the problem of how to optimize the composition of enzyme cocktails with respect to this. The degree of synergy (DS) may change with substrate conversion, and some studies have suggested a maximum in DS early in the process. Here, we systematically investigated interrelationships of DS and conversion in a model system covering a wide range of experimental conditions. The results did not reveal any correlation between DS and contact time, but when plotted against the degree of substrate conversion we saw a systematic increase in DS. We suggest that this is linked to a decreasing reactivity of the substrate. Hence, synergy became increasingly important as the recalcitrance of the remaining substrate grew. Such conversion dependent changes in DS appear to be important both in mechanistic studies and attempts to find industrial enzymes blends with optimal synergy. Biotechnol. Bioeng. 2017;114: 696-700. © 2016 Wiley Periodicals, Inc.
[Mh] Termos MeSH primário: Celulase/química
Celulase/metabolismo
Celulose/metabolismo
Proteínas Fúngicas/química
Proteínas Fúngicas/metabolismo
[Mh] Termos MeSH secundário: Celulose/análise
Hidrólise
Hypocrea/enzimologia
Cinética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungal Proteins); 9004-34-6 (Cellulose); EC 3.2.1.4 (Cellulase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160913
[St] Status:MEDLINE
[do] DOI:10.1002/bit.26179


  6 / 176 MEDLINE  
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[PMID]:27377383
[Au] Autor:Gudmundsson M; Hansson H; Karkehabadi S; Larsson A; Stals I; Kim S; Sunux S; Fujdala M; Larenas E; Kaper T; Sandgren M
[Ad] Endereço:Department of Chemistry and Biotechnology, Swedish University of Agricultural Sciences, Box 7015, 750 07 Uppsala, Sweden.
[Ti] Título:Structural and functional studies of the glycoside hydrolase family 3 ß-glucosidase Cel3A from the moderately thermophilic fungus Rasamsonia emersonii.
[So] Source:Acta Crystallogr D Struct Biol;72(Pt 7):860-70, 2016 07.
[Is] ISSN:2059-7983
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The filamentous fungus Hypocrea jecorina produces a number of cellulases and hemicellulases that act in a concerted fashion on biomass and degrade it into monomeric or oligomeric sugars. ß-Glucosidases are involved in the last step of the degradation of cellulosic biomass and hydrolyse the ß-glycosidic linkage between two adjacent molecules in dimers and oligomers of glucose. In this study, it is shown that substituting the ß-glucosidase from H. jecorina (HjCel3A) with the ß-glucosidase Cel3A from the thermophilic fungus Rasamsonia emersonii (ReCel3A) in enzyme mixtures results in increased efficiency in the saccharification of lignocellulosic materials. Biochemical characterization of ReCel3A, heterologously produced in H. jecorina, reveals a preference for disaccharide substrates over longer gluco-oligosaccharides. Crystallographic studies of ReCel3A revealed a highly N-glycosylated three-domain dimeric protein, as has been observed previously for glycoside hydrolase family 3 ß-glucosidases. The increased thermal stability and saccharification yield and the superior biochemical characteristics of ReCel3A compared with HjCel3A and mixtures containing HjCel3A make ReCel3A an excellent candidate for addition to enzyme mixtures designed to operate at higher temperatures.
[Mh] Termos MeSH primário: Eurotiales/enzimologia
beta-Glucosidase/química
beta-Glucosidase/metabolismo
[Mh] Termos MeSH secundário: Cristalografia por Raios X
Eurotiales/química
Eurotiales/metabolismo
Proteínas Fúngicas/química
Proteínas Fúngicas/metabolismo
Glicosilação
Hidrólise
Hypocrea/química
Hypocrea/enzimologia
Hypocrea/metabolismo
Lignina/metabolismo
Modelos Moleculares
Conformação Proteica
Multimerização Proteica
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Fungal Proteins); 11132-73-3 (lignocellulose); 9005-53-2 (Lignin); EC 3.2.1.21 (beta-Glucosidase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171117
[Lr] Data última revisão:
171117
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160706
[St] Status:MEDLINE
[do] DOI:10.1107/S2059798316008482


  7 / 176 MEDLINE  
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[PMID]:27328173
[Au] Autor:Ren F; Zhu S; Wang B; Li L; Liu X; Su R; Che Y
[Ad] Endereço:State Key Laboratory of Toxicology & Medical Countermeasures, Beijing Institute of Pharmacology & Toxicology , Beijing 100850, People's Republic of China.
[Ti] Título:Hypocriols A-F, Heterodimeric Botryane Ethers from Hypocrea sp., an Insect-Associated Fungus.
[So] Source:J Nat Prod;79(7):1848-56, 2016 Jul 22.
[Is] ISSN:1520-6025
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The new heterodimeric botryane ethers hypocriols A-F (1-6) and the known compounds 4ß-acetoxy-9ß,10ß,15α-trihydroxyprobotrydial (7), dihydrobotrydial (8), 10-oxodehydrodihydrobotrydial (9), and dehydrobotrydienol (10) were isolated from the solid cultures of an insect-associated fungus Hypocrea sp. The structures of 1-6 were elucidated primarily by NMR experiments. The absolute configuration of 1 was assigned using the modified Mosher method and electronic circular dichroism (ECD) calculations, whereas those for 3-5, and 2 and 6 were deduced via ECD calculations and circular dichroism data, respectively. Compounds 1-6 appear to be the first heterodimeric botryane ethers and showed antiproliferative effects against a small panel of four human tumor cell lines.
[Mh] Termos MeSH primário: Antineoplásicos/isolamento & purificação
Hypocrea/química
Sesquiterpenos/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Antineoplásicos/química
Antineoplásicos/farmacologia
China
Ensaios de Seleção de Medicamentos Antitumorais
Éteres
Células HCT116
Células HeLa
Seres Humanos
Estrutura Molecular
Mariposas/microbiologia
Sesquiterpenos/química
Sesquiterpenos/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (10-oxodehydrodihydrobotrydial); 0 (Antineoplastic Agents); 0 (Ethers); 0 (Sesquiterpenes)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170510
[Lr] Data última revisão:
170510
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160622
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jnatprod.6b00394


  8 / 176 MEDLINE  
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[PMID]:27083360
[Au] Autor:Jonathan MC; van Brussel M; Scheffers MS; Kabel MA
[Ad] Endereço:Laboratory of Food Chemistry, Wageningen University, Bornse Weilanden 9, 6708 WG Wageningen, The Netherlands. Electronic address: melliana.jonathan@wur.nl.
[Ti] Título:Different action patterns of glucoamylases on branched gluco-oligosaccharides from amylopectin.
[So] Source:Carbohydr Polym;143:198-203, 2016 Jun 05.
[Is] ISSN:1879-1344
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A bottleneck in enzymatic starch hydrolysis, like in biofuel industry, is relatively slow degradation of branched structures compared to linear ones. This research aimed to evaluate glucoamylases for their activity towards branched gluco-oligosaccharides. The activity of seven modified glucoamylases and two homologs was compared to that of a reference glucoamylase obtained from a commercial enzyme cocktail 'Distillase® SSF'. All enzymes were evaluated for their activity towards panose (glc(α1-6)glc(α1-4)glc), pullulan and a purified branched gluco-oligosaccharide with a degree of polymerisation of 5 (bDP5) identified as glc(α1-4)[glc(α1-4)glc(α1-6)]glc(α1-4)glc. The enzymes degraded bDP5 differently, which was mainly due to variation in their capability to cleave α-(1→6)-linked or the α-(1→4)-linked glucosyl residue at the non-reducing end of the branched glucosyl residue. By comparing the enzyme activity towards bDP5 with those towards panose and pullulan, it was suggested that the activity towards bDP5 could be estimated only when the activity towards both commercial substrates was evaluated.
[Mh] Termos MeSH primário: Amilopectina/química
Glucana 1,4-alfa-Glucosidase/química
Oligossacarídeos/química
[Mh] Termos MeSH secundário: Sequência de Carboidratos
Glucanos/química
Glucose/análise
Hypocrea/enzimologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glucans); 0 (Oligosaccharides); 33401-87-5 (panose); 8ZQ0AYU1TT (pullulan); 9037-22-3 (Amylopectin); EC 3.2.1.3 (Glucan 1,4-alpha-Glucosidase); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170213
[Lr] Data última revisão:
170213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160417
[St] Status:MEDLINE


  9 / 176 MEDLINE  
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[PMID]:26828743
[Au] Autor:Ding LJ; Yuan W; Li YX; Liao XJ; Sun H; Peng Q; Han BN; Lin HW; Li ZY; Yang F; Xu SH
[Ad] Endereço:a College of Pharmacy , Jinan University , Guangzhou , PR China.
[Ti] Título:Hypocrol A, a new tyrosol derivative from a sponge-derived strain of the fungus Hypocrea koningii.
[So] Source:Nat Prod Res;30(14):1633-8, 2016 Jul.
[Is] ISSN:1478-6427
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In continuation of our search for new antibacterial and antioxidant metabolites from sponge-derived fungi, one new tyrosol derivative, hypocrol A (1), together with four known congeners, trichodenol B (2), 4-hydroxyphenethyl acetate (3), 4-hydroxyphenethyl tetradecanoate (4) and 1-oleyltyrosol (5), was isolated from the strain Hypocrea koningii PF04. Their planar structures were unequivocally elucidated by spectroscopic methods and comparison with the literature data. All the compounds displayed weak antibacterial activities against Staphylococcus aureus, methicillin-resistant S. aureus and Escherichia coli, whereas compounds 1 and 2 exhibited a moderate antioxidant efficacy in the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay with IC50 values of 48.5 and 97.4 µg/mL, respectively.
[Mh] Termos MeSH primário: Hypocrea/química
Álcool Feniletílico/análogos & derivados
Poríferos/microbiologia
[Mh] Termos MeSH secundário: Animais
Antibacterianos/farmacologia
Antioxidantes/farmacologia
Compostos de Bifenilo
Cromatografia Líquida de Alta Pressão
Escherichia coli/efeitos dos fármacos
Fermentação
Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos
Testes de Sensibilidade Microbiana
Álcool Feniletílico/química
Álcool Feniletílico/isolamento & purificação
Álcool Feniletílico/farmacologia
Picratos
Espectrometria de Massas por Ionização por Electrospray
Espectrofotometria Infravermelho
Espectrofotometria Ultravioleta
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Antioxidants); 0 (Biphenyl Compounds); 0 (Picrates); 0 (hypocrol A); 1AK4MU3SNX (4-hydroxyphenylethanol); DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl); ML9LGA7468 (Phenylethyl Alcohol)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160202
[St] Status:MEDLINE
[do] DOI:10.1080/14786419.2015.1129333


  10 / 176 MEDLINE  
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[PMID]:26477388
[Au] Autor:Bengtsson O; Arntzen MØ; Mathiesen G; Skaugen M; Eijsink VGH
[Ad] Endereço:Norwegian University of Life Sciences, NMBU, Department of Chemistry, Biotechnology and Food Science, P.O. Box 5003, NO-1432 Aas, Norway. Electronic address: oskar.bengtsson@nmbu.no.
[Ti] Título:A novel proteomics sample preparation method for secretome analysis of Hypocrea jecorina growing on insoluble substrates.
[So] Source:J Proteomics;131:104-112, 2016 Jan 10.
[Is] ISSN:1876-7737
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Analysis of the secretomes of filamentous fungi growing on insoluble lignocellulosic substrates is of major current interest because of the industrial potential of secreted fungal enzymes. Importantly, such studies can help identifying key enzymes from a large arsenal of bioinformatically detected candidates in fungal genomes. We describe a simple, plate-based method to analyze the secretome of Hypocrea jecorina growing on insoluble substrates that allows harsh sample preparation methods promoting desorption, and subsequent identification, of substrate-bound proteins, while minimizing contamination with non-secreted proteins from leaking or lysed cells. The validity of the method was demonstrated by comparative secretome analysis of wild-type H.jecorina strain QM6a growing on bagasse, birch wood, spruce wood or pure cellulose, using label-fee quantification. The proteomic data thus obtained were consistent with existing data from transcriptomics and proteomics studies and revealed clear differences in the responses to complex lignocellulosic substrates and the response to pure cellulose. This easy method is likely to be generally applicable to filamentous fungi and to other microorganisms growing on insoluble substrates.
[Mh] Termos MeSH primário: Proteínas de Bactérias/secreção
Sistema Livre de Células/secreção
Hypocrea/metabolismo
Lignina/metabolismo
Proteoma/secreção
Manejo de Espécimes/métodos
[Mh] Termos MeSH secundário: Proliferação Celular/fisiologia
Solubilidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Proteome); 11132-73-3 (lignocellulose); 9005-53-2 (Lignin)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:170729
[Lr] Data última revisão:
170729
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151020
[St] Status:MEDLINE



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