Base de dados : MEDLINE
Pesquisa : B01.300.107.575 [Categoria DeCS]
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  1 / 1169 MEDLINE  
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[PMID]:29248352
[Au] Autor:Li D; Luong TTM; Dan WJ; Ren Y; Nien HX; Zhang AL; Gao JM
[Ad] Endereço:Shaanxi Key Laboratory of Natural Products & Chemical Biology, Shaanxi Engineering Center of Bioresource Chemistry & Sustainable Utilization, College of Chemistry & Pharmacy, Northwest A&F University, Yangling 712100, China.
[Ti] Título:Natural products as sources of new fungicides (IV): Synthesis and biological evaluation of isobutyrophenone analogs as potential inhibitors of class-II fructose-1,6-bisphosphate aldolase.
[So] Source:Bioorg Med Chem;26(2):386-393, 2018 01 15.
[Is] ISSN:1464-3391
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Several recently identified antifungal compounds share the backbone structure of acetophenones. The aim of the present study was to develop new isobutyrophenone analogs as new antifungal agents. A series of new 2,4-dihydroxy-5-methyl isobutyrophenone derivatives were prepared and characterized by H, C NMR and MS spectroscopic data. These products were evaluated for in vitro antifungal activities against seven plant fungal pathogens by the mycelial growth inhibitory rate assay. Compounds 3, 4a, 5a, 5b, 5e, 5f and 5g showed a broad-spectrum high antifungal activity. On the other hand, for the first time, these compounds were also assayed as potential inhibitors against Class II fructose-1,6-bisphosphate aldolase (Fba) from the rice blast fungus, Magnaporthe grisea. Compounds 5e and 5g were found to exhibit the inhibition constants (Ki) for 15.12 and 14.27 µM, respectively, as the strongest competitive inhibitors against Fba activity. The possible binding-modes of compounds 5e and 5g were further analyzed by molecular docking algorithms. The results strongly suggested that compound 5g could be a promising lead for the discovery of new fungicides via targeting Class II Fba.
[Mh] Termos MeSH primário: Antifúngicos/farmacologia
Produtos Biológicos/farmacologia
Butirofenonas/farmacologia
Inibidores Enzimáticos/farmacologia
Frutose-Bifosfato Aldolase/antagonistas & inibidores
Magnaporthe/efeitos dos fármacos
[Mh] Termos MeSH secundário: Antifúngicos/síntese química
Antifúngicos/química
Produtos Biológicos/síntese química
Produtos Biológicos/química
Butirofenonas/síntese química
Butirofenonas/química
Relação Dose-Resposta a Droga
Inibidores Enzimáticos/síntese química
Inibidores Enzimáticos/química
Frutose-Bifosfato Aldolase/metabolismo
Magnaporthe/enzimologia
Magnaporthe/crescimento & desenvolvimento
Testes de Sensibilidade Microbiana
Simulação de Acoplamento Molecular
Estrutura Molecular
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Biological Products); 0 (Butyrophenones); 0 (Enzyme Inhibitors); EC 4.1.2.13 (Fructose-Bisphosphate Aldolase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171218
[St] Status:MEDLINE


  2 / 1169 MEDLINE  
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[PMID]:28742127
[Au] Autor:Galhano R; Illana A; Ryder LS; Rodríguez-Romero J; Demuez M; Badaruddin M; Martinez-Rocha AL; Soanes DM; Studholme DJ; Talbot NJ; Sesma A
[Ad] Endereço:Disease & Stress Biology Dept. John Innes Centre, Norwich, United Kingdom.
[Ti] Título:Tpc1 is an important Zn(II)2Cys6 transcriptional regulator required for polarized growth and virulence in the rice blast fungus.
[So] Source:PLoS Pathog;13(7):e1006516, 2017 Jul.
[Is] ISSN:1553-7374
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The establishment of polarity is a critical process in pathogenic fungi, mediating infection-related morphogenesis and host tissue invasion. Here, we report the identification of TPC1 (Transcription factor for Polarity Control 1), which regulates invasive polarized growth in the rice blast fungus Magnaporthe oryzae. TPC1 encodes a putative transcription factor of the fungal Zn(II)2Cys6 family, exclusive to filamentous fungi. Tpc1-deficient mutants show severe defects in conidiogenesis, infection-associated autophagy, glycogen and lipid metabolism, and plant tissue colonisation. By tracking actin-binding proteins, septin-5 and autophagosome components, we show that Tpc1 regulates cytoskeletal dynamics and infection-associated autophagy during appressorium-mediated plant penetration. We found that Tpc1 interacts with Mst12 and modulates its DNA-binding activity, while Tpc1 nuclear localisation also depends on the MAP kinase Pmk1, consistent with the involvement of Tpc1 in this signalling pathway, which is critical for appressorium development. Importantly, Tpc1 directly regulates NOXD expression, the p22phox subunit of the fungal NADPH oxidase complex via an interaction with Mst12. Tpc1 therefore controls spatial and temporal regulation of cortical F-actin through regulation of the NADPH oxidase complex during appressorium re-polarisation. Consequently, Tpc1 is a core developmental regulator in filamentous fungi, linking the regulated synthesis of reactive oxygen species and the Pmk1 pathway, with polarity control during host invasion.
[Mh] Termos MeSH primário: Proteínas Fúngicas/metabolismo
Magnaporthe/metabolismo
Magnaporthe/patogenicidade
Oryza/microbiologia
Doenças das Plantas/microbiologia
Esporos Fúngicos/crescimento & desenvolvimento
Fatores de Transcrição/metabolismo
Zinco/metabolismo
[Mh] Termos MeSH secundário: Polaridade Celular
Proteínas Fúngicas/genética
Regulação Fúngica da Expressão Gênica
Magnaporthe/genética
Magnaporthe/crescimento & desenvolvimento
Ligação Proteica
Esporos Fúngicos/enzimologia
Esporos Fúngicos/genética
Esporos Fúngicos/metabolismo
Fatores de Transcrição/genética
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungal Proteins); 0 (Transcription Factors); J41CSQ7QDS (Zinc)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171213
[Lr] Data última revisão:
171213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170726
[St] Status:MEDLINE
[do] DOI:10.1371/journal.ppat.1006516


  3 / 1169 MEDLINE  
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[PMID]:29016662
[Au] Autor:Dong L; Liu S; Xu P; Deng W; Li X; Tharreau D; Li J; Zhou J; Wang Q; Tao D; Yang Q
[Ad] Endereço:Agricultural Environment and Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan, China.
[Ti] Título:Fine mapping of Pi57(t) conferring broad spectrum resistance against Magnaporthe oryzae in introgression line IL-E1454 derived from Oryza longistaminata.
[So] Source:PLoS One;12(10):e0186201, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Wild species of the genus Oryza are excellent gene pools for improvement of agronomic traits of Asian cultivated rice. The blast resistance gene Pi57(t) in the introgression line IL-E1454 derived from Oryza longistaminata was previously mapped on rice chromosome 12. Inoculation with 322 Magnaporthe oryzae isolates collected from 6 countries indicated that Pi57(t) conferred broad spectrum resistance against M. oryzae. Two mapping populations consisting of 29070 and 10375 F2 plants derived from the crosses of resistant donor IL-E1454 with susceptible parents RD23 and Lijiangxintuanheigu respectively, were used for fine mapping of Pi57(t) locus. Based on genotyping and phenotyping results of recombinants screened from the two crosses, Pi57(t) was finally mapped to a 51.7-kb region flanked by two molecular markers (STS57-320 and STS57-372) on the short arm and close to the centromere of chromosome 12. Six candidate resistance genes were predicted in the target region according to the reference sequence of Nipponbare. These results could facilitate both marker-assisted selection for disease-resistant breeding and gene cloning.
[Mh] Termos MeSH primário: Mapeamento Cromossômico/métodos
Resistência à Doença/genética
Genoma de Planta
Oryza/genética
[Mh] Termos MeSH secundário: Cruzamentos Genéticos
Loci Gênicos
Marcadores Genéticos
Genótipo
Magnaporthe/crescimento & desenvolvimento
Magnaporthe/patogenicidade
Oryza/microbiologia
Fenótipo
Melhoramento Vegetal
Doenças das Plantas/genética
Doenças das Plantas/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Genetic Markers)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171011
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186201


  4 / 1169 MEDLINE  
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[PMID]:28873459
[Au] Autor:Cen K; Li B; Lu Y; Zhang S; Wang C
[Ad] Endereço:CAS Key Laboratory of Insect Developmental and Evolutionary Biology, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, China.
[Ti] Título:Divergent LysM effectors contribute to the virulence of Beauveria bassiana by evasion of insect immune defenses.
[So] Source:PLoS Pathog;13(9):e1006604, 2017 Sep.
[Is] ISSN:1553-7374
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The lysin motif (LysM) containing proteins can bind chitin and are ubiquitous in various organisms including fungi. In plant pathogenic fungi, a few LysM proteins have been characterized as effectors to suppress chitin-induced immunity in plant hosts and therefore contribute to fungal virulence. The effector mechanism is still questioned in fungus-animal interactions. In this study, we found that LysM proteins are also present in animal pathogenic fungi and have evolved divergently. The genome of the insect pathogen Beauveria bassiana encodes 12 LysM proteins, and the genes were differentially transcribed by the fungus when grown in different conditions. Deletion of six genes that were expressed by the fungus growing in insects revealed that two, Blys2 and Blys5, were required for full fungal virulence. Both proteins could bind chitin and Blys5 (containing two LysM domains) could additionally bind chitosan and cellulose. Truncation analysis of Blys2 (containing five LysM domains) indicated that the combination of LysM domains could determine protein-binding affinity and specificity for different carbohydrates. Relative to the wild-type strain, loss of Blys2 or Blys5 could impair fungal propagation in insect hemocoels and lead to the upregulation of antifungal gene in insects. Interestingly, the virulence defects of ΔBlys2 and ΔBlys5 could be fully restored by complementation with the Slp1 effector from the rice blast fungus Magnaporthe oryzae. In contrast to Slp1 and Blys2, Blys5 could potentially protect fungal hyphae against chitinase hydrolysis. The results of this study not only advance the understanding of LysM protein evolution but also establish the effector mechanism of fungus-animal interactions.
[Mh] Termos MeSH primário: Beauveria/imunologia
Genes Fúngicos/imunologia
Magnaporthe/imunologia
Mucoproteínas/isolamento & purificação
[Mh] Termos MeSH secundário: Magnaporthe/genética
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Mucoproteins); 0 (lysin, gastropoda)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170906
[St] Status:MEDLINE
[do] DOI:10.1371/journal.ppat.1006604


  5 / 1169 MEDLINE  
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[PMID]:28807829
[Au] Autor:Zhang Y; Liang Y; Dong Y; Gao Y; Yang X; Yuan J; Qiu D
[Ad] Endereço:The State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 12 Zhong-guan-cun South Street, Beijing 100081, China.
[Ti] Título:The Magnaporthe oryzae Alt A 1-like protein MoHrip1 binds to the plant plasma membrane.
[So] Source:Biochem Biophys Res Commun;492(1):55-60, 2017 Oct 07.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:MoHrip1, a protein isolated from Magnaporthe oryzae, belongs to the Alt A 1 (AA1) family. mohrip1 mRNA levels showed inducible expression throughout the infection process in rice. To determine the location of MoHrip1 in M. oryzae, a mohrip1-gfp mutant was generated. Fluorescence microscopy observations and western blotting analysis showed that MoHrip1 was both present in the secretome and abundant in the fungal cell wall. To obtain MoHrip1 protein, we carried out high-yield expression of MoHrip1 in Pichia pastoris. Treatment of tobacco plants with MoHrip1 induced the formation of necrosis, accumulation of reactive oxygen species and expression of several defense-related genes, as well as conferred disease resistance. By fusion to green fluorescent protein, we showed that MoHrip1 was able to bind to the tobacco and rice plant plasma membrane, causing rapid morphological changes at the cellular level, such as cell shrinkage and chloroplast disorganization. These findings indicate that MoHrip1 is a microbe-associated molecular pattern that is perceived by the plant immune system. This is the first study on an AA1 family protein that can bind to the plant plasma membrane.
[Mh] Termos MeSH primário: Membrana Celular/metabolismo
Proteínas Fúngicas/metabolismo
Magnaporthe/química
Tabaco/citologia
[Mh] Termos MeSH secundário: Membrana Celular/química
Proteínas Fúngicas/biossíntese
Proteínas Fúngicas/imunologia
Proteínas Fúngicas/isolamento & purificação
Magnaporthe/imunologia
Padrões Moleculares Associados a Patógenos
Doenças das Plantas/imunologia
Doenças das Plantas/microbiologia
Imunidade Vegetal
Tabaco/imunologia
Tabaco/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungal Proteins); 0 (Pathogen-Associated Molecular Pattern Molecules)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170816
[St] Status:MEDLINE


  6 / 1169 MEDLINE  
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[PMID]:28806765
[Au] Autor:Li Y; Zhang X; Hu S; Liu H; Xu JR
[Ad] Endereço:Purdue-NWAFU Joint Research Center, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi, China.
[Ti] Título:PKA activity is essential for relieving the suppression of hyphal growth and appressorium formation by MoSfl1 in Magnaporthe oryzae.
[So] Source:PLoS Genet;13(8):e1006954, 2017 Aug.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In the rice blast fungus Magnaporthe oryzae, the cAMP-PKA pathway regulates surface recognition, appressorium turgor generation, and invasive growth. However, deletion of CPKA failed to block appressorium formation and responses to exogenous cAMP. In this study, we generated and characterized the cpk2 and cpkA cpk2 mutants and spontaneous suppressors of cpkA cpk2 in M. oryzae. Our results demonstrate that CPKA and CPK2 have specific and overlapping functions, and PKA activity is essential for appressorium formation and plant infection. Unlike the single mutants, the cpkA cpk2 mutant was significantly reduced in growth and rarely produced conidia. It failed to form appressoria although the intracellular cAMP level and phosphorylation of Pmk1 MAP kinase were increased. The double mutant also was defective in plant penetration and Mps1 activation. Interestingly, it often produced fast-growing spontaneous suppressors that formed appressoria but were still non-pathogenic. Two suppressor strains of cpkA cpk2 had deletion and insertion mutations in the MoSFL1 transcription factor gene. Deletion of MoSFL1 or its C-terminal 93-aa (MoSFL1ΔCT) was confirmed to suppress the defects of cpkA cpk2 in hyphal growth but not appressorium formation or pathogenesis. We also isolated 30 spontaneous suppressors of the cpkA cpk2 mutant in Fusarium graminearum and identified mutations in 29 of them in FgSFL1. Affinity purification and co-IP assays showed that this C-terminal region of MoSfl1 was essential for its interaction with the conserved Cyc8-Tup1 transcriptional co-repressor, which was reduced by cAMP treatment. Furthermore, the S211D mutation at the conserved PKA-phosphorylation site in MoSFL1 partially suppressed the defects of cpkA cpk2. Overall, our results indicate that PKA activity is essential for appressorium formation and proper activation of Pmk1 or Mps1 in M. oryzae, and phosphorylation of MoSfl1 by PKA relieves its interaction with the Cyc8-Tup1 co-repressor and suppression of genes important for hyphal growth.
[Mh] Termos MeSH primário: Proteínas Quinases Dependentes de AMP Cíclico/metabolismo
AMP Cíclico/metabolismo
Proteínas Fúngicas/metabolismo
Magnaporthe/crescimento & desenvolvimento
Oryza/microbiologia
Fatores de Transcrição/metabolismo
[Mh] Termos MeSH secundário: Proteínas Quinases Dependentes de AMP Cíclico/genética
Proteínas Fúngicas/genética
Deleção de Genes
Regulação Fúngica da Expressão Gênica
Interações Hidrofóbicas e Hidrofílicas
Magnaporthe/enzimologia
Magnaporthe/genética
Proteínas Quinases Ativadas por Mitógeno/genética
Proteínas Quinases Ativadas por Mitógeno/metabolismo
Mutação
Fosforilação
Doenças das Plantas/microbiologia
Transdução de Sinais
Fatores de Transcrição/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungal Proteins); 0 (Transcription Factors); E0399OZS9N (Cyclic AMP); EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases); EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170913
[Lr] Data última revisão:
170913
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170815
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1006954


  7 / 1169 MEDLINE  
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[PMID]:28719243
[Au] Autor:Mgonja EM; Park CH; Kang H; Balimponya EG; Opiyo S; Bellizzi M; Mutiga SK; Rotich F; Ganeshan VD; Mabagala R; Sneller C; Correll J; Zhou B; Talbot NJ; Mitchell TK; Wang GL
[Ad] Endereço:First, second, fifth, sixth, ninth, fifteenth, and sixteenth authors: Department of Plant Pathology, The Ohio State University, Columbus; fourth and eleventh authors: Department of Horticulture and Crop science, The Ohio State University, Columbus; third author: State Key Laboratory for Biology of P
[Ti] Título:Genotyping-by-Sequencing-Based Genetic Analysis of African Rice Cultivars and Association Mapping of Blast Resistance Genes Against Magnaporthe oryzae Populations in Africa.
[So] Source:Phytopathology;107(9):1039-1046, 2017 09.
[Is] ISSN:0031-949X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Understanding the genetic diversity of rice germplasm is important for the sustainable use of genetic materials in rice breeding and production. Africa is rich in rice genetic resources that can be utilized to boost rice productivity on the continent. A major constraint to rice production in Africa is rice blast, caused by the hemibiotrophic fungal pathogen Magnaporthe oryzae. In this report, we present the results of a genotyping-by-sequencing (GBS)-based diversity analysis of 190 African rice cultivars and an association mapping of blast resistance (R) genes and quantitative trait loci (QTLs). The 190 African cultivars were clustered into three groups based on the 184K single nucleotide polymorphisms generated by GBS. We inoculated the rice cultivars with six African M. oryzae isolates. Association mapping identified 25 genomic regions associated with blast resistance (RABRs) in the rice genome. Moreover, PCR analysis indicated that RABR_23 is associated with the Pi-ta gene on chromosome 12. Our study demonstrates that the combination of GBS-based genetic diversity population analysis and association mapping is effective in identifying rice blast R genes/QTLs that contribute to resistance against African populations of M. oryzae. The identified markers linked to the RABRs and 14 highly resistant cultivars in this study will be useful for rice breeding in Africa.
[Mh] Termos MeSH primário: Genótipo
Magnaporthe/fisiologia
Oryza/genética
Oryza/imunologia
Doenças das Plantas/imunologia
Doenças das Plantas/microbiologia
[Mh] Termos MeSH secundário: África
Filogenia
Locos de Características Quantitativas
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170719
[St] Status:MEDLINE
[do] DOI:10.1094/PHYTO-12-16-0421-R


  8 / 1169 MEDLINE  
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[PMID]:28542408
[Au] Autor:Li L; Chen X; Zhang S; Yang J; Chen D; Liu M; Zhang H; Zheng X; Wang P; Peng Y; Zhang Z
[Ad] Endereço:Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, and Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China.
[Ti] Título:MoCAP proteins regulated by MoArk1-mediated phosphorylation coordinate endocytosis and actin dynamics to govern development and virulence of Magnaporthe oryzae.
[So] Source:PLoS Genet;13(5):e1006814, 2017 May.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Actin organization is a conserved cellular process that regulates the growth and development of eukaryotic cells. It also governs the virulence process of pathogenic fungi, such as the rice blast fungus Magnaporthe oryzae, with mechanisms not yet fully understood. In a previous study, we found that actin-regulating kinase MoArk1 displays conserved functions important in endocytosis and actin organization, and MoArk1 is required for maintaining the growth and full virulence of M. oryzae. To understand how MoArk1 might function, we identified capping protein homologs from M. oryzae (MoCAP) that interact with MoArk1 in vivo. MoCAP is heterodimer consisting of α and ß subunits MoCapA and MoCapB. Single and double deletions of MoCAP subunits resulted in abnormal mycelial growth and conidia formation. The ΔMocap mutants also exhibited reduced appressorium penetration and invasive hyphal growth within host cells. Furthermore, the ΔMocap mutants exhibited delayed endocytosis and abnormal cytoskeleton assembly. Consistent with above findings, MoCAP proteins interacted with MoAct1, co-localized with actin during mycelial development, and participated in appressorial actin ring formation. Further analysis revealed that the S85 residue of MoCapA and the S285 residue of MoCapB were subject to phosphorylation by MoArk1 that negatively regulates MoCAP functions. Finally, the addition of exogenous phosphatidylinositol 4,5-bisphosphate (PIP2) failed to modulate actin ring formation in ΔMocap mutants, in contrast to the wild-type strain, suggesting that MoCAP may also mediate phospholipid signaling in the regulation of the actin organization. These results together demonstrate that MoCAP proteins whose functions are regulated by MoArk1 and PIP2 are important for endocytosis and actin dynamics that are directly linked to growth, conidiation and pathogenicity of M. oryzae.
[Mh] Termos MeSH primário: Proteínas de Capeamento de Actina/metabolismo
Actinas/metabolismo
Endocitose
Proteínas Fúngicas/metabolismo
Magnaporthe/metabolismo
Processamento de Proteína Pós-Traducional
Proteínas Serina-Treonina Quinases/metabolismo
[Mh] Termos MeSH secundário: Proteínas de Capeamento de Actina/genética
Magnaporthe/crescimento & desenvolvimento
Magnaporthe/patogenicidade
Micélio/crescimento & desenvolvimento
Fosfatidilinositol 4,5-Difosfato/metabolismo
Fosforilação
Proteínas Serina-Treonina Quinases/genética
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Actin Capping Proteins); 0 (Actins); 0 (Fungal Proteins); 0 (Phosphatidylinositol 4,5-Diphosphate); EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170628
[Lr] Data última revisão:
170628
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170526
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1006814


  9 / 1169 MEDLINE  
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[PMID]:28521637
[Au] Autor:Kanda Y; Yokotani N; Maeda S; Nishizawa Y; Kamakura T; Mori M
[Ad] Endereço:a Institute of Agrobiological Sciences, NARO (NIAS) , Tsukuba , Japan.
[Ti] Título:The receptor-like cytoplasmic kinase BSR1 mediates chitin-induced defense signaling in rice cells.
[So] Source:Biosci Biotechnol Biochem;81(8):1497-1502, 2017 Aug.
[Is] ISSN:1347-6947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Broad-Spectrum Resistance 1 (BSR1) encodes a rice receptor-like cytoplasmic kinase, and enhances disease resistance when overexpressed. Rice plants overexpressing BSR1 are highly resistant to diverse pathogens, including rice blast fungus. However, the mechanism responsible for this resistance has not been fully characterized. To analyze the BSR1 function, BSR1-knockout (BSR1-KO) plants were generated using a clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system. Experiments using suspension-cultured cells revealed that defense responses including H O production (i.e. oxidative burst) and expression of defense-related genes induced by autoclaved conidia of the rice blast fungus significantly decreased in BSR1-KO cells. Furthermore, a treatment with chitin oligomers which function as microbe-associated molecular patterns (MAMPs) of the rice blast fungus resulted in considerably suppressed defense responses in BSR1-KO cells. These results suggest that BSR1 is important for the rice innate immunity triggered by the perception of chitin.
[Mh] Termos MeSH primário: Quitina/imunologia
Resistência à Doença/genética
Regulação da Expressão Gênica de Plantas
Oryza/imunologia
Doenças das Plantas/imunologia
Transdução de Sinais/imunologia
[Mh] Termos MeSH secundário: Sequência de Bases
Sistemas CRISPR-Cas
Técnicas de Cultura de Células
Quitina/genética
Técnicas de Inativação de Genes
Peróxido de Hidrogênio/imunologia
Peróxido de Hidrogênio/metabolismo
Magnaporthe/patogenicidade
Magnaporthe/fisiologia
Oryza/genética
Oryza/microbiologia
Células Vegetais/imunologia
Células Vegetais/metabolismo
Células Vegetais/microbiologia
Doenças das Plantas/genética
Doenças das Plantas/microbiologia
Imunidade Vegetal/genética
Proteínas de Plantas/genética
Proteínas de Plantas/imunologia
Plantas Geneticamente Modificadas
Proteínas Serina-Treonina Quinases/genética
Proteínas Serina-Treonina Quinases/imunologia
Transdução de Sinais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Proteins); 1398-61-4 (Chitin); BBX060AN9V (Hydrogen Peroxide); EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170726
[Lr] Data última revisão:
170726
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170520
[St] Status:MEDLINE
[do] DOI:10.1080/09168451.2017.1325710


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[PMID]:28445532
[Au] Autor:Yadav MK; S A; Ngangkham U; Shubudhi HN; Bag MK; Adak T; Munda S; Samantaray S; Jena M
[Ad] Endereço:ICAR-National Rice Research Institute, Cuttack, Odisha, India.
[Ti] Título:Use of molecular markers in identification and characterization of resistance to rice blast in India.
[So] Source:PLoS One;12(4):e0176236, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Rice blast disease caused by Magnaporthe oryzae is one of the most destructive disease causing huge losses to rice yield in different parts of the world. Therefore, an attempt has been made to find out the resistance by screening and studying the genetic diversity of eighty released rice varieties by National Rice Research Institute, Cuttack (NRVs) using molecular markers linked to twelve major blast resistance (R) genes viz Pib, Piz, Piz-t, Pik, Pik-p, Pikm Pik-h, Pita/Pita-2, Pi2, Pi9, Pi1 and Pi5. Out of which, nineteen varieties (23.75%) showed resistance, twenty one were moderately resistant (26.25%) while remaining forty varieties (50%) showed susceptible in uniform blast nursery. Rice varieties possessing blast resistance genes varied from four to twelve and the frequencies of the resistance genes ranged from 0 to 100%. The cluster analysis grouped the eighty NRVs into two major clusters at 63% level of genetic similarity coefficient. The PIC value for seventeen markers varied from 0 to 0.37 at an average of 0.20. Out of seventeen markers, only five markers, 195R-1, Pi9-i, Pita3, YL155/YL87 and 40N23r corresponded to three broad spectrum R genes viz. Pi9, Pita/Pita2 and Pi5 were found to be significantly associated with the blast disease with explaining phenotypic variance from 3.5% to 7.7%. The population structure analysis and PCoA divided the entire 80 NRVs into two sub-groups. The outcome of this study would help to formulate strategies for improving rice blast resistance through genetic studies, plant-pathogen interaction, identification of novel R genes, development of new resistant varieties through marker-assisted breeding for improving rice blast resistance in India and worldwide.
[Mh] Termos MeSH primário: Resistência à Doença/genética
Genes de Plantas
Marcadores Genéticos/genética
Magnaporthe/patogenicidade
Oryza/genética
[Mh] Termos MeSH secundário: Alelos
Análise por Conglomerados
Frequência do Gene
Variação Genética
Índia
Fenótipo
Doenças das Plantas/genética
Proteínas de Plantas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Genetic Markers); 0 (Plant Proteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170427
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0176236



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