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[PMID]:28240390
[Au] Autor:Wang P; Kenyon C; de Hoog S; Guo L; Fan H; Liu H; Li Z; Sheng R; Yang Y; Jiang Y; Zhang L; Xu Y
[Ad] Endereço:Division of Clinical Microbiology, P.U.M.C.H., Beijing, China.
[Ti] Título:A novel dimorphic pathogen, Emergomyces orientalis (Onygenales), agent of disseminated infection.
[So] Source:Mycoses;60(5):310-319, 2017 May.
[Is] ISSN:1439-0507
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:A novel dimorphic fungus, Emergomyces orientalis sp. nov. a close relative of systemic pathogens in the family Ajellomycetaceae (Blastomyces, Histoplasma). The fungus is reported in a 64-year-old male from Shanxi, China. The patient developed disseminated skin lesions, productive cough with fever and showed nodular opacities in his left lung on chest radiography. The patient had no identified cause of immunodeficiency apart from type-2 diabetes mellitus. Clinical, histopathological and mycological characteristics of the agent are given, and its phylogenetic position is determined with multilocus sequence data.
[Mh] Termos MeSH primário: Infecções Fúngicas Invasivas/diagnóstico
Infecções Fúngicas Invasivas/microbiologia
Onygenales/isolamento & purificação
Onygenales/patogenicidade
Filogenia
[Mh] Termos MeSH secundário: Blastomyces/genética
China
DNA Ribossômico
Diabetes Mellitus Tipo 2/complicações
Diabetes Mellitus Tipo 2/microbiologia
Febre/etiologia
Febre/microbiologia
Histoplasma/genética
Seres Humanos
Pulmão/diagnóstico por imagem
Pulmão/microbiologia
Pulmão/patologia
Masculino
Meia-Idade
Tipagem de Sequências Multilocus
Onygenales/classificação
Onygenales/genética
Análise de Sequência de DNA
Pele/microbiologia
Pele/patologia
Tomografia Computadorizada por Raios X
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Ribosomal)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170424
[Lr] Data última revisão:
170424
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170228
[St] Status:MEDLINE
[do] DOI:10.1111/myc.12583


  2 / 81 MEDLINE  
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[PMID]:28176377
[Au] Autor:Dukik K; Muñoz JF; Jiang Y; Feng P; Sigler L; Stielow JB; Freeke J; Jamalian A; Gerrits van den Ende B; McEwen JG; Clay OK; Schwartz IS; Govender NP; Maphanga TG; Cuomo CA; Moreno LF; Kenyon C; Borman AM; de Hoog S
[Ad] Endereço:CBS-KNAW Fungal Biodiversity Centre, Utrecht, The Netherlands.
[Ti] Título:Novel taxa of thermally dimorphic systemic pathogens in the Ajellomycetaceae (Onygenales).
[So] Source:Mycoses;60(5):296-309, 2017 May.
[Is] ISSN:1439-0507
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Recent discoveries of novel systemic fungal pathogens with thermally dimorphic yeast-like phases have challenged the current taxonomy of the Ajellomycetaceae, a family currently comprising the genera Blastomyces, Emmonsia, Emmonsiellopsis, Helicocarpus, Histoplasma, Lacazia and Paracoccidioides. Our morphological, phylogenetic and phylogenomic analyses demonstrated species relationships and their specific phenotypes, clarified generic boundaries and provided the first annotated genome assemblies to support the description of two new species. A new genus, Emergomyces, accommodates Emmonsia pasteuriana as type species, and the new species Emergomyces africanus, the aetiological agent of case series of disseminated infections in South Africa. Both species produce small yeast cells that bud at a narrow base at 37°C and lack adiaspores, classically associated with the genus Emmonsia. Another novel dimorphic pathogen, producing broad-based budding cells at 37°C and occurring outside North America, proved to belong to the genus Blastomyces, and is described as Blastomyces percursus.
[Mh] Termos MeSH primário: Micoses/microbiologia
Onygenales/classificação
Onygenales/genética
[Mh] Termos MeSH secundário: Blastomyces/genética
Chrysosporium/genética
Genoma Fúngico
Histoplasma/genética
Seres Humanos
Microscopia
Micélio/ultraestrutura
Micoses/epidemiologia
América do Norte/epidemiologia
Onygenales/patogenicidade
Onygenales/ultraestrutura
Fenótipo
Filogenia
Análise de Sequência de DNA
África do Sul/epidemiologia
Esporos Fúngicos/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170424
[Lr] Data última revisão:
170424
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170209
[St] Status:MEDLINE
[do] DOI:10.1111/myc.12601


  3 / 81 MEDLINE  
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[PMID]:27079795
[Au] Autor:Humphrey S; Alexander S; Ha HJ
[Ad] Endereço:a Animal Health Laboratory, Ministry for Primary Industries , 66 Ward Street, Wallaceville, Upper Hutt , New Zealand.
[Ti] Título:Detection of Paranannizziopsis australasiensis in tuatara (Sphenodon punctatus) using fungal culture and a generic fungal PCR.
[So] Source:N Z Vet J;64(5):298-300, 2016 Sep.
[Is] ISSN:1176-0710
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:AIMS: To describe the methods used at the Animal Health Laboratory (AHL, Ministry for Primary Industries) to identify Paranannizziopsis australasiensis. METHODS: Skin biopsy samples from two adult male tuatara were submitted to the AHL in March 2014. Approximately half of each sample was processed for fungal culture and incubated on mycobiotic agar containing cycloheximide at 30°C. Following morphological examination of the culture products, DNA was extracted from suspect colonies. PCR was used to amplify the internal transcribed spacer (ITS) region of fungal rRNA using primers ITS1 and ITS4. Positive amplicons were subjected to DNA sequencing and the results were compared to published sequences. In addition, DNA was extracted from the remaining skin samples and the same PCR was carried out to compare the results. RESULTS: After 7 days of incubation, colonies morphologically resembling P. australasiensis were observed. DNA extracted from these isolates tested positive for P. australasiensis by PCR and DNA sequencing. Samples of DNA extracted directly from the infected skin samples tested negative for P. australasiensis using the generic fungal PCR. CONCLUSIONS AND CLINICAL RELEVANCE: Isolation and identification of P. australasiensis was carried out using a combination of fungal culture and molecular testing available at AHL. Results were available in significantly less time than in the past, when isolates had to be sent overseas. PCR and sequencing of fungal isolates is a valuable tool for identification of species that have few, if any, unique macroscopic or microscopic features to aid identification. Further sampling from captive and wild New Zealand reptiles will provide important information on the epidemiology of P. australasiensis, and the conservation and management implications for tuatara and other native reptile species.
[Mh] Termos MeSH primário: Dermatomicoses/veterinária
Onygenales/genética
Répteis/microbiologia
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
DNA Fúngico/genética
Dermatomicoses/microbiologia
Masculino
Onygenales/isolamento & purificação
Reação em Cadeia da Polimerase/veterinária
Pele/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Fungal)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170206
[Lr] Data última revisão:
170206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160416
[St] Status:MEDLINE
[do] DOI:10.1080/00480169.2016.1177472


  4 / 81 MEDLINE  
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[PMID]:27074995
[Au] Autor:Masters NJ; Alexander S; Jackson B; Sigler L; Chatterton J; Harvey C; Gibson R; Humphrey S; Rawdon TG; Spence RP; Ha HJ; McInnes K; Jakob-Hoff R
[Ad] Endereço:a Zoological Society of London , London NW1 4RY , United Kingdom.
[Ti] Título:Dermatomycosis caused by Paranannizziopsis australasiensis in five tuatara (Sphenodon punctatus) and a coastal bearded dragon (Pogona barbata) in a zoological collection in New Zealand.
[So] Source:N Z Vet J;64(5):301-7, 2016 Sep.
[Is] ISSN:1176-0710
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:CASE HISTORY: Health monitoring of tuatara (Sphenodon punctatus) at Auckland Zoo between 2001 and 2009 showed that 58/93 tuatara had been affected by dermatitis of unknown origin. From 2011 onwards, cases of suspected fungal dermatitis underwent extensive diagnostic investigations. CLINCAL FINDINGS: Six cases of dermatomycosis were attributed to Paranannizziopsis australasiensis, five in tuatara and one in a coastal bearded dragon (Pogona barbata). Cases presented typically as raised, yellow to brown encrustations on the skin. Severe cases progressed to necrotising ulcerative dermatitis, and in the bearded dragon to fatal systemic mycosis. Following topical and systemic treatments, lesions resolved in all five tuatara. LABORATORY FINDINGS: Histopathological examination of skin biopsy samples revealed dermatitis with intralesional septate branching hyphae. Fungal culture yielded isolates morphologically resembling Chrysosporium species, and isolates were submitted for molecular confirmation and sequencing of DNA. DIAGNOSIS: All six cases were confirmed as dermatitis due to infection with P. australasiensis, on the basis of fungal culture and DNA sequencing of isolates. CLINICAL RELEVANCE: These are the first reported cases of dermatomycosis associated with P. australasiensis infection in tuatara, and the first cases in which systemic therapeutic agents have been used in the treatment of such disease. Tuatara at the Auckland Zoo are now routinely examined every 3 months and tissue samples from any lesions sent for histopathology and fungal culture. Further work to elucidate the epidemiology and significance of P. australasiensis infections in reptiles in New Zealand is important for both welfare and conservation purposes.
[Mh] Termos MeSH primário: Dermatomicoses/veterinária
Lagartos/microbiologia
Onygenales
Répteis/microbiologia
[Mh] Termos MeSH secundário: Animais
Animais de Zoológico/microbiologia
Dermatomicoses/microbiologia
Feminino
Masculino
Nova Zelândia
Reação em Cadeia da Polimerase/veterinária
Pele/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170206
[Lr] Data última revisão:
170206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160415
[St] Status:MEDLINE
[do] DOI:10.1080/00480169.2016.1177473


  5 / 81 MEDLINE  
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[PMID]:26758910
[Au] Autor:Nenoff P; Schorlemmer B; Uhrlaß S; Baunacke A; Baunacke A; Friedrichs C; Iffländer J; Syhre E; Schneider A; Krüger C; Maier T
[Ad] Endereço:Labor für medizinische Mikrobiologie, Mölbiser Hauptstraße 8, 04571, Rötha/OT Mölbis, Deutschland. nenoff@mykologie-experten.de.
[Ti] Título:[Onychocola canadensis Sigler in onychomycosis : A new dermatophyte-like mould in Germany].
[Ti] Título:Onychocola canadensis Sigler bei Onychomykose : Ein neuer Dermatophyten-ähnlicher Schimmelpilz in Deutschland..
[So] Source:Hautarzt;67(9):739-49, 2016 Sep.
[Is] ISSN:1432-1173
[Cp] País de publicação:Germany
[La] Idioma:ger
[Ab] Resumo:Moulds or non-dermatophyte moulds (NDM) are being increasingly isolated as causative agent of onychomycoses. Known causes of a NDM-OM are Scopulariopsis brevicaulis, Fusarium, Aspergillus, Acremonium, Neoscytalidium dimidiatum, Arthrographis kalrae, and Chaetomium. In this article, 5 patients with suspected nail infection due to Onychocola canadensis are reported for the first time in Germany. Systemic antifungal agents are not considered to be effective in NDM onychomycosis. In individual cases, however, terbinafine seems to be effective in Onychocola canadensis infection of the nails. Treatment of choice represents, however, nontraumatic nail avulsion using 40 % urea ointment followed by antifungal nail lacquer with ciclopirox olamine or amorolfine.
[Mh] Termos MeSH primário: Naftalenos/uso terapêutico
Onicomicose/diagnóstico
Onicomicose/tratamento farmacológico
Onygenales/isolamento & purificação
[Mh] Termos MeSH secundário: Idoso
Antifúngicos/uso terapêutico
Doenças Transmissíveis Emergentes/diagnóstico
Doenças Transmissíveis Emergentes/tratamento farmacológico
Doenças Transmissíveis Emergentes/microbiologia
Feminino
Alemanha
Seres Humanos
Masculino
Meia-Idade
Onicomicose/microbiologia
Resultado do Tratamento
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Naphthalenes); G7RIW8S0XP (terbinafine)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170916
[Lr] Data última revisão:
170916
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160114
[St] Status:MEDLINE
[do] DOI:10.1007/s00105-015-3755-1


  6 / 81 MEDLINE  
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[PMID]:26745835
[Au] Autor:Guthrie AL; Knowles S; Ballmann AE; Lorch JM
[Ad] Endereço:1 Virginia Zoo, 3500 Granby St., Norfolk, Virginia 23504, USA.
[Ti] Título:Detection of Snake Fungal Disease Due to Ophidiomyces ophiodiicola in Virginia, USA.
[So] Source:J Wildl Dis;52(1):143-9, 2016 Jan.
[Is] ISSN:1943-3700
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Snake fungal disease (SFD) is an emerging disease of wildlife believed to be caused by Ophidiomyces ophiodiicola. Although geographic and host ranges have yet to be determined, this disease is characterized by crusty scales, superficial pustules, and subcutaneous nodules, with subsequent morbidity and mortality in some snake species. To confirm the presence of SFD and O. ophiodiicola in snakes of eastern Virginia, US, we clinically examined 30 free-ranging snakes on public lands from April to October 2014. Skin biopsy samples were collected from nine snakes that had gross lesions suggestive of SFD; seven of these biopsies were suitable for histologic interpretation, and eight were suitable for culture and PCR detection of O. ophiodiicola. Seven snakes had histologic features consistent with SFD and eight were positive for O. ophiodiicola by PCR or fungal culture.
[Mh] Termos MeSH primário: Micoses/veterinária
Onygenales/isolamento & purificação
Serpentes
[Mh] Termos MeSH secundário: Animais
Feminino
Masculino
Micoses/microbiologia
Micoses/patologia
Onygenales/genética
Onygenales/patogenicidade
Pele/microbiologia
Pele/patologia
Virginia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160109
[St] Status:MEDLINE
[do] DOI:10.7589/2015-04-093.1


  7 / 81 MEDLINE  
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[PMID]:26686929
[Au] Autor:Niu S; Si L; Liu D; Zhou A; Zhang Z; Shao Z; Wang S; Zhang L; Zhou D; Lin W
[Ad] Endereço:State Key Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing, 100191, PR China; Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, SOA, Xiamen, 361005, PR China.
[Ti] Título:Spiromastilactones: A new class of influenza virus inhibitors from deep-sea fungus.
[So] Source:Eur J Med Chem;108:229-244, 2016 Jan 27.
[Is] ISSN:1768-3254
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:A new class of phenolic lactones with trivial names of spiromastilactones A-M (1-13) was isolated from a deep-sea derived Spiromastix sp. fungus. Their structures featured by various chlorination at aromatic rings were determined on the basis of extensive spectroscopic analyses. An antiviral assay revealed that most of the tested compounds exert inhibitory activity against WSN influenza virus with low cytotoxicity, while the structure-activity relationships were discussed. Spiromastilactone D (4), a 5'-chloro-2'-methoxy substituted analogue, displayed the most potent to inhibit a panel of influenza A and B viruses in addition to drug-resistant clinical isolates. Mechanistic investigation resulted in that compound 4 bonded to hemagglutinin protein (HA), potentially at the spherical head, and disrupted the HA-sialic acid receptor interaction, that is essential for the attachment and entry of all influenza viruses. In addition, compound 4 also showed inhibitory effect toward viral genome replication via targeting viral RNP complex. The synergistic effects of 4 on both viral entry and replication assumed it to be a promising lead for the development of a new influenza inhibitor.
[Mh] Termos MeSH primário: Antivirais/farmacologia
Organismos Aquáticos/química
Benzofuranos/farmacologia
Vírus da Influenza A/efeitos dos fármacos
Lactonas/farmacologia
Onygenales/química
[Mh] Termos MeSH secundário: Animais
Antivirais/química
Antivirais/isolamento & purificação
Benzofuranos/química
Benzofuranos/isolamento & purificação
Cães
Relação Dose-Resposta a Droga
Lactonas/química
Lactonas/isolamento & purificação
Células Madin Darby de Rim Canino
Testes de Sensibilidade Microbiana
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antiviral Agents); 0 (Benzofurans); 0 (Lactones); 0 (spiromastilactone D)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:170726
[Lr] Data última revisão:
170726
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151222
[St] Status:MEDLINE


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[PMID]:26577613
[Au] Autor:Hirooka Y; Tanney JB; Nguyen HD; Seifert KA
[Ad] Endereço:Biodiversity (Mycology), Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, Ottawa, Ontario, K1A 0C6 Canada; Department of Biology, University of Ottawa, Ottawa, Ontario, K1N 6N5 Canada yuurihirooka@gmail.com.
[Ti] Título:Xerotolerant fungi in house dust: taxonomy of Spiromastix, Pseudospiromastix and Sigleria gen. nov. in Spiromastigaceae (Onygenales, Eurotiomycetes).
[So] Source:Mycologia;108(1):135-56, 2016 Jan-Feb.
[Is] ISSN:0027-5514
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:During a global investigation of fungi in house dust, we isolated six novel arthroconidial fungi. Phylogenies from combined analysis of nuc rDNA 18S, 28S and internal transcribed spacers sequences demonstrated that these fungi and two species preserved in culture collections represent undescribed species of Spiromastigaceae, Onygenales. Seven of the eight species lacked sexual states and only characters of asexual states and growth rates on different media could be used to characterize them. The eighth species produced ascomata only on water agar. We introduce six new species and one new combination in Spiromastix and validate the recently proposed family Spiromastigaceae, genus Pseudospiromastix and combination Ps. tentaculata. The new genus Sigleria is proposed for two new species that differ from Spiromastix by conidiophore branching patterns, slower growth and a limited ability to utilize nitrate as a sole N source. A key to the three genera of Spiromastigaceae, Spiromastix, Pseudospiromastix and Sigleria, is provided. Phylogenetic analyses support the placement of Spiromastigaceae within Onygenales.
[Mh] Termos MeSH primário: Onygenales/classificação
[Mh] Termos MeSH secundário: Sequência de Bases
DNA Fúngico/química
DNA Fúngico/genética
DNA Ribossômico/química
DNA Ribossômico/genética
DNA Espaçador Ribossômico/química
DNA Espaçador Ribossômico/genética
Poeira
Dados de Sequência Molecular
Onygenales/citologia
Onygenales/genética
Onygenales/crescimento & desenvolvimento
Filogenia
Análise de Sequência de DNA
Especificidade da Espécie
Esporos Fúngicos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Fungal); 0 (DNA, Ribosomal); 0 (DNA, Ribosomal Spacer); 0 (Dust)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:160216
[Lr] Data última revisão:
160216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151119
[St] Status:MEDLINE
[do] DOI:10.3852/15-065


  9 / 81 MEDLINE  
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[PMID]:26490705
[Au] Autor:Zhou N; Zhang Y; Liu F; Cai L
[Ad] Endereço:State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China University of Chinese Academy of Sciences, Beijing 100049, China.
[Ti] Título:Halophilic and thermotolerant Gymnoascus species from several special environments, China.
[So] Source:Mycologia;108(1):179-91, 2016 Jan-Feb.
[Is] ISSN:0027-5514
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This study introduces three new Gymnoascus species (Gymnoascaceae, Onygenales), G. halophilus, G. stercorarius and G. thermotolerans, isolated from sediments in Chaka Salt Lake, compost and cornfield soil, respectively, in China, based on a polyphasic characterization including morphology, physiology and molecular phylogeny. Phylogenetic relationships were assessed based on the nuclear internal transcribed spacer (ITS = ITS1 + 5.8S + ITS2) region and a combined multilocus alignment of the ITS, 18S subunit rRNA gene and 28S subunit rRNA genes. Our study identified phylogenetic and phenotypic characters that differentiated the three new species from known species in the genus. Salinity and temperature tolerance tests revealed that G. halophilus was an obligate halophile while G. stercorarius and G. thermotolerans were halotolerant and thermotolerant. A key to accepted species of Gymnoascus is provided.
[Mh] Termos MeSH primário: Onygenales/classificação
[Mh] Termos MeSH secundário: Sequência de Bases
China
DNA Fúngico/química
DNA Fúngico/genética
DNA Ribossômico/química
DNA Ribossômico/genética
DNA Espaçador Ribossômico/química
DNA Espaçador Ribossômico/genética
Temperatura Alta
Dados de Sequência Molecular
Onygenales/citologia
Onygenales/genética
Filogenia
Plantas Tolerantes a Sal
Análise de Sequência de DNA
Esporos Fúngicos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Fungal); 0 (DNA, Ribosomal); 0 (DNA, Ribosomal Spacer)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:160216
[Lr] Data última revisão:
160216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151023
[St] Status:MEDLINE
[do] DOI:10.3852/15-086


  10 / 81 MEDLINE  
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[PMID]:26476165
[Au] Autor:Mahajan C; Basotra N; Singh S; Di Falco M; Tsang A; Chadha BS
[Ad] Endereço:Department of Microbiology, Guru Nanak Dev University, Amritsar 143005, Punjab, India. Electronic address: chhaviosho@yahoo.com.
[Ti] Título:Malbranchea cinnamomea: A thermophilic fungal source of catalytically efficient lignocellulolytic glycosyl hydrolases and metal dependent enzymes.
[So] Source:Bioresour Technol;200:55-63, 2016 Jan.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This study reports thermophilic fungus Malbranchea cinnamomea as an important source of lignocellulolytic enzymes. The secretome analysis using LC-MS/MS orbitrap showed that fungus produced a spectrum of glycosyl hydrolases (cellulase/hemicellulase), polysaccharide lyases (PL) and carbohydrate esterases (CE) in addition to cellobiose dehydrogenase (CDH) indicating the presence of functional classical and oxidative cellulolytic mechanisms. The protein fractions in the secretome resolved by ion exchange chromatography were analyzed for ability to hydrolyze alkali treated carrot grass (ATCG) in the presence of Mn(2+)/Cu(2+). This strategy in tandem with peptide mass fingerprinting led to identification of metal dependent protein hydrolases with no apparent hydrolytic activity, however, showed 5.7 folds higher saccharification in presence of Mn(2+). Furthermore, adding different protein fractions to commercial cellulase (Novozymes: Cellic CTec2) resulted in enhanced hydrolysis of ATCG ranging between 1.57 and 3.43 folds indicating the enzymes from M. cinnamomea as catalytically efficient.
[Mh] Termos MeSH primário: Asteraceae/metabolismo
Proteínas Fúngicas/metabolismo
Glicosídeo Hidrolases/metabolismo
Onygenales/enzimologia
[Mh] Termos MeSH secundário: Asteraceae/química
Desidrogenases de Carboidrato/química
Desidrogenases de Carboidrato/metabolismo
Catálise
Celulase/química
Celulase/metabolismo
Proteínas Fúngicas/química
Glicosídeo Hidrolases/química
Hidrólise
Metais/metabolismo
Plantas Daninhas/química
Plantas Daninhas/metabolismo
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Fungal Proteins); 0 (Metals); EC 1.1.- (Carbohydrate Dehydrogenases); EC 1.1.99.18 (cellobiose-quinone oxidoreductase); EC 3.2.1.- (Glycoside Hydrolases); EC 3.2.1.- (hemicellulase); EC 3.2.1.4 (Cellulase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151018
[St] Status:MEDLINE



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