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[PMID]:28408215
[Au] Autor:Barbosa A; Reiss A; Jackson B; Warren K; Paparini A; Gillespie G; Stokeld D; Irwin P; Ryan U
[Ad] Endereço:School of Veterinary and Life Sciences, Murdoch University, Murdoch, Perth, WA 6150, Australia; CAPES Foundation, Ministry of Education of Brazil, Brasília, DF 70040-020, Brazil,. Electronic address: A.DuarteBarbosa@murdoch.edu.au.
[Ti] Título:Prevalence, genetic diversity and potential clinical impact of blood-borne and enteric protozoan parasites in native mammals from northern Australia.
[So] Source:Vet Parasitol;238:94-105, 2017 Apr 30.
[Is] ISSN:1873-2550
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A molecular survey was conducted to provide baseline information on the prevalence, genetic diversity and potential clinical impacts of blood-borne and enteric protozoans in native wild mammals from the Northern Territory (NT). A total of 209 blood and 167 faecal samples were collected from four target species; the northern brown bandicoot (Isoodon macrourus), common brushtail possum (Trichosurus vulpecula), northern quoll (Dasyurus hallucatus) and brush-tailed rabbit-rat (Conilurus penicillatus). Blood samples were screened by PCR at the 18S rRNA gene for trypanosomes, piroplasms and haemogregarines, with faecal samples tested for Cryptosporidium spp. at the 18S rRNA locus, and for Giardia spp. at the glutamate dehydrogenase (gdh) and 18S rRNA loci. The potential clinical impact was investigated by associating clinical, haematological and biochemical parameters with presence or absence of infection. Overall, 22.5% (95% CI: 17.0-28.8%) of the animals tested were positive for haemoprotozoans. Trypanosomes were found in 26.6% (95% CI: 18.7-35.7%) of the bandicoots and were identified as Trypanosoma vegrandis G6, except for one unique genotype, most similar to T. vegrandis G3 (genetic distance=7%). The prevalence of trypanosomes in possums was 23.7% (95% CI: 11.4-40.2%), and the genotypes identified clustered within the T. noyesi clade. The presence of Babesia sp. and Hepatozoon sp. was confirmed in bandicoots only, both at a prevalence of 9.7% (95% CI: 2.7-9.2%). The total prevalence of intestinal protozoan parasites observed was relatively low (3%; 95% CI: 1.0-6.9%). No evidence of clinical disease associated with protozoan parasitic infection was observed, however bandicoots positive for Trypanosoma exhibited a significantly lower packed cell volume (PCV) compared to negative bandicoots (p=0.046). To the authors' knowledge, this is the first research conducted in the NT to characterise protozoan parasites in threatened native mammals using both molecular and morphological tools; and to assess the potential clinical impacts of these agents. The absence of clear signs of major morbidity in infected animals seems to exclude a direct association between infections with these agents and possible population decline events in northern Australian native mammals. However until the cause(s) of population decline are ascertained for each individual mammal species, further studies are required. The outcome of the present investigation may be used to inform wildlife conservation and zoonotic disease programs.
[Mh] Termos MeSH primário: Patógenos Transmitidos pelo Sangue/classificação
Eucariotos/genética
Variação Genética
Marsupiais/parasitologia
Infecções Protozoárias em Animais/parasitologia
[Mh] Termos MeSH secundário: Animais
Austrália/epidemiologia
Eucariotos/classificação
Eucariotos/isolamento & purificação
Parasitemia
Phyllachorales
Filogenia
Infecções Protozoárias em Animais/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170415
[St] Status:MEDLINE


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[PMID]:28286910
[Au] Autor:Xu H; Wang N; Liu J; Qu C; Wang Y; Jiang S; Lu N; Wang D; Zhang Z; Chen X
[Ad] Endereço:National Key Laboratory of Crop Biology, College of Horticulture Science, Shandong Agricultural University, Tai-An, Shandong, China.
[Ti] Título:The molecular mechanism underlying anthocyanin metabolism in apple using the MdMYB16 and MdbHLH33 genes.
[So] Source:Plant Mol Biol;94(1-2):149-165, 2017 May.
[Is] ISSN:1573-5028
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:KEY MESSAGE: MdMYB16 forms homodimers and directly inhibits anthocyanin synthesis via its C-terminal EAR repressor. It weakened the inhibitory effect of MdMYB16 on anthocyanin synthesis when overexpressing MdbHLH33 in callus overexpressing MdMYB16. MdMYB16 could interact with MdbHLH33. Anthocyanins are strong antioxidants that play a key role in the prevention of cardiovascular disease, cancer, and diabetes. The germplasm of Malus sieversii f. neidzwetzkyana is important for the study of anthocyanin metabolism. To date, only limited studies have examined the negative regulatory mechanisms underlying anthocyanin synthesis in apple. Here, we analyzed the relationship between anthocyanin levels and MdMYB16 expression in mature Red Crisp 1-5 apple (M. domestica) fruit, generated an evolutionary tree, and identified an EAR suppression sequence and a bHLH binding motif of the MdMYB16 protein using protein sequence analyses. Overexpression of MdMYB16 or MdMYB16 without bHLH binding sequence (LBSMdMYB16) in red-fleshed callus inhibited MdUFGT and MdANS expression and anthocyanin synthesis. However, overexpression of MdMYB16 without the EAR sequence (LESMdMYB16) in red-fleshed callus had no inhibitory effect on anthocyanin. The yeast one-hybrid assay showed that MdMYB16 and LESMdMYB16 interacted the promoters of MdANS and MdUFGT, respectively. Yeast two-hybrid, pull-down, and bimolecular fluorescence complementation assays showed that MdMYB16 formed homodimers and interacted with MdbHLH33, however, the LBSMdMYB16 could not interact with MdbHLH33. We overexpressed MdbHLH33 in callus overexpressing MdMYB16 and found that it weakened the inhibitory effect of MdMYB16 on anthocyanin synthesis. Together, these results suggested that MdMYB16 and MdbHLH33 may be important part of the regulatory network controlling the anthocyanin biosynthetic pathway.
[Mh] Termos MeSH primário: Antocianinas/metabolismo
Regulação da Expressão Gênica de Plantas/fisiologia
Malus/metabolismo
Proteínas de Plantas/metabolismo
Fatores de Transcrição/metabolismo
[Mh] Termos MeSH secundário: Antocianinas/genética
Clonagem Molecular
Frutas
Técnicas de Inativação de Genes
Malus/genética
Phyllachorales
Proteínas de Plantas/genética
Fatores de Transcrição/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthocyanins); 0 (Plant Proteins); 0 (Transcription Factors)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170314
[St] Status:MEDLINE
[do] DOI:10.1007/s11103-017-0601-0


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[PMID]:27447804
[Au] Autor:Bui DK; Carvounis PE
[Ad] Endereço:1 Department of Ophthalmology, Cullen Eye Institute , Baylor College of Medicine, Houston, Texas.
[Ti] Título:Favorable Outcomes of Filamentous Fungal Endophthalmitis Following Aggressive Management.
[So] Source:J Ocul Pharmacol Ther;32(9):623-630, 2016 Nov.
[Is] ISSN:1557-7732
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PURPOSE: To evaluate the visual outcomes following aggressive management of filamentous fungal endophthalmitis with prompt surgical intervention and oral and intravitreal voriconazole. METHODS: Retrospective chart review study of consecutive patients with culture- or biopsy-proven filamentous fungal endophthalmitis treated at an academic referral center. Clinical characteristics, treatment regimens, and visual outcomes were analyzed. RESULTS: Included were 5 patients, 1 with endogenous endophthalmitis due to systemic fusariosis and 4 due to exogenous endophthalmitis (1 with Fusarium, 2 with Scedosporium apiospermum, and 1 with Glomerella spp.). On presentation, 1 patient had best-corrected visual acuity (BCVA) of 20/20. The remaining 4 patients had count-fingers to hand motion (HM) vision. All patients underwent immediate surgical intervention for infection control. All patients received oral or intravenous voriconazole and aggressive intravitreal voriconazole every 2-3 days initially. Intravitreal amphotericin was added if there was poor response to voriconazole alone. Three patients achieved a final BCVA of 20/20, 1 patient achieved BCVA of 20/50, and 1 remained HMs only. CONCLUSION: Aggressive treatment of filamentous fungal endophthalmitis with early surgical intervention, systemic antifungal therapy, and frequent intravitreal injections of voriconazole can result in excellent visual outcomes in some patients.
[Mh] Termos MeSH primário: Anfotericina B/farmacologia
Antifúngicos/farmacologia
Endoftalmite/tratamento farmacológico
Infecções Oculares Fúngicas/tratamento farmacológico
Voriconazol/farmacologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Anfotericina B/administração & dosagem
Antifúngicos/administração & dosagem
Endoftalmite/microbiologia
Infecções Oculares Fúngicas/diagnóstico
Infecções Oculares Fúngicas/microbiologia
Feminino
Fusarium/efeitos dos fármacos
Seres Humanos
Injeções Intravítreas
Masculino
Testes de Sensibilidade Microbiana
Meia-Idade
Phyllachorales/efeitos dos fármacos
Estudos Retrospectivos
Scedosporium/efeitos dos fármacos
Resultado do Tratamento
Voriconazol/administração & dosagem
Adulto Jovem
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 7XU7A7DROE (Amphotericin B); JFU09I87TR (Voriconazole)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171108
[Lr] Data última revisão:
171108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160723
[St] Status:MEDLINE


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[PMID]:27139585
[Au] Autor:Zhang Y; Shi X; Li B; Zhang Q; Liang W; Wang C
[Ad] Endereço:College of Agronomy and Plant Protection, Key Lab of Integrated Crop Pest Management of Shandong Province, Qingdao Agricultural University, Qingdao, 266109, PR China.
[Ti] Título:Salicylic acid confers enhanced resistance to Glomerella leaf spot in apple.
[So] Source:Plant Physiol Biochem;106:64-72, 2016 Sep.
[Is] ISSN:1873-2690
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Glomerella leaf spot (GLS) caused by Glomerella cingulata is a newly emergent disease that results in severe defoliation and fruit spots in apple. Currently, there are no effective means to control this disease except for the traditional fungicide sprays. Induced resistance by elicitors against pathogens infection is a widely accepted eco-friendly strategy. In the present study, we investigated whether exogenous application of salicylic acid (SA) could improve resistance to GLS in a highly susceptible apple cultivar (Malus domestica Borkh. cv. 'Gala') and the underlying mechanisms. The results showed that pretreatment with SA, at 0.1-1.0 mM, induced strong resistance against GLS in 'Gala' apple leaves, with SA treated leaves showing significant reduction in lesion numbers and disease index. Concurrent with the enhanced disease resistance, SA treatment markedly increased the total antioxidant capacity (T-AOC) and defence-related enzyme activities, including catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), phenylalanine ammonia-lyase (PAL) and polyphenol oxidase (PPO). As expected, SA treatment also induced the expression levels of five pathogenesis-related (PR) genes including PR1, PR5, PR8, Chitinase and ß-1,3-glucanase. Furthermore, the most pronounced and/or rapid increase was observed in leaves treated with SA and subsequently inoculated with G. cingulata compared to the treatment with SA or inoculation with the pathogen. Together, these results suggest that exogenous SA triggered increase in reactive oxygen species levels and the antioxidant system might be responsible for enhanced resistance against G. cingulata in 'Gala' apple leaves.
[Mh] Termos MeSH primário: Resistência à Doença/efeitos dos fármacos
Malus/imunologia
Malus/microbiologia
Phyllachorales/fisiologia
Doenças das Plantas/microbiologia
Folhas de Planta/microbiologia
Ácido Salicílico/farmacologia
[Mh] Termos MeSH secundário: Antioxidantes/metabolismo
Catalase/metabolismo
Catecol Oxidase/metabolismo
Quitinases/genética
Quitinases/metabolismo
Regulação da Expressão Gênica de Plantas/efeitos dos fármacos
Glicosídeo Hidrolases/genética
Glicosídeo Hidrolases/metabolismo
Peróxido de Hidrogênio/metabolismo
Malus/efeitos dos fármacos
Malus/genética
Peroxidases/metabolismo
Fenilalanina Amônia-Liase/metabolismo
Phyllachorales/efeitos dos fármacos
Phyllachorales/crescimento & desenvolvimento
Folhas de Planta/efeitos dos fármacos
Folhas de Planta/enzimologia
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Reação em Cadeia da Polimerase em Tempo Real
Superóxido Dismutase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (RNA, Messenger); BBX060AN9V (Hydrogen Peroxide); EC 1.10.3.1 (Catechol Oxidase); EC 1.11.1.- (Peroxidases); EC 1.11.1.6 (Catalase); EC 1.15.1.1 (Superoxide Dismutase); EC 3.2.1.- (Glycoside Hydrolases); EC 3.2.1.14 (Chitinases); EC 4.3.1.24 (Phenylalanine Ammonia-Lyase); O414PZ4LPZ (Salicylic Acid)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170327
[Lr] Data última revisão:
170327
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160504
[St] Status:MEDLINE


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[PMID]:27025344
[Au] Autor:Shi W; Dan WJ; Tang JJ; Zhang Y; Nandinsuren T; Zhang AL; Gao JM
[Ad] Endereço:Shaanxi Key Labotory of Natural Products & Chemical Biology, College of Science, Northwest A&F University, Yangling 712100, People's Republic of China.
[Ti] Título:Natural products as sources of new fungicides (III): Antifungal activity of 2,4-dihydroxy-5-methylacetophenone derivatives.
[So] Source:Bioorg Med Chem Lett;26(9):2156-8, 2016 May 01.
[Is] ISSN:1464-3405
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A series of new 2,4-dihydroxy-5-methylacetophenone 2 derivatives were synthesized, and characterized by (1)H, (13)C NMR and ESI-MS. Their antifungal activities were evaluated in vitro against five important plant fungal pathogens including Cytospora sp., Glomerella cingulate, Pyricularia oryzaecar, Botrytis cinerea and Alternaria solani by the mycelial growth inhibitory rate assay. Compounds 2b-d, 2g and 2h displayed a broad-spectrum activity. The logP value of these active compounds is ranging from 1.71 to 2.54. Especially, isopropyl ketone 2g (logP 2.27) was found to be the most active to the tested organisms with IC50 values of 17.28-32.32 µg/mL. The results suggest that compound 2g might be a promising candidate in the development of new agrochemical antifungal agents. Preliminary structure-activity relationship (SAR) studies of the acetophenone derivatives are also discussed.
[Mh] Termos MeSH primário: Acetofenonas/farmacologia
Fungicidas Industriais
[Mh] Termos MeSH secundário: Acetofenonas/síntese química
Acetofenonas/química
Fungicidas Industriais/síntese química
Fungicidas Industriais/química
Concentração Inibidora 50
Fungos Mitospóricos
Phyllachorales
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (2,4-dihydroxy-5-methylacetophenone); 0 (Acetophenones); 0 (Fungicides, Industrial)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170721
[Lr] Data última revisão:
170721
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160331
[St] Status:MEDLINE


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[PMID]:27019063
[Au] Autor:Rockenbach MF; Velho AC; Gonçalves AE; Mondino PE; Alaniz SM; Stadnik MJ
[Ad] Endereço:First, second, third, and sixth authors: Laboratory of Plant Pathology, Agricultural Science Center, Federal University of Santa Catarina, Rod. Admar Gonzaga 1346, 88034-001 Florianópolis-SC, Brazil; and fourth and fifth authors: Department of Plant Protection, Faculty of Agronomy, University of the
[Ti] Título:Genetic Structure of Colletotrichum fructicola Associated to Apple Bitter Rot and Glomerella Leaf Spot in Southern Brazil and Uruguay.
[So] Source:Phytopathology;106(7):774-81, 2016 Jul.
[Is] ISSN:0031-949X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Colletotrichum fructicola is the main species causing apple bitter rot (ABR) and Glomerella leaf spot (GLS) in southern Brazil, and ABR in Uruguay where GLS remains unnoticed. Thus, this work aimed to determine the genetic structure of C. fructicola isolates of both the countries. A total of 28 out of 31 Brazilian isolates (90.3%) caused typical symptoms of GLS, while only 6 of 25 Uruguayan isolates (24.0%) originating from fruits were able to infect leaves, but causing atypical symptoms. Both populations showed similar levels of Nei's gene diversity (h = 0.088 and 0.079, for Brazilian and Uruguayan populations, respectively), and Bayesian cluster analysis inferred two genetic clusters correlated with the geographical origin of isolates. A principal coordinates analysis scatter plot and an unweighted pair group method with arithmetic mean-based dendrogram also grouped Brazilian and Uruguayan isolates into two groups. By pairwise comparison of nitrate-nonutilizing (nit) mutants with a proposed set of testers, all Uruguayan isolates were grouped into a unique vegetative compatibility group (namely VCG 1), while Brazilian isolates were grouped into four VCGs (VCG 1 to 4). Brazilian and Uruguayan populations of C. fructicola were found to be genetically distinct. Our results suggest that isolates of C. fructicola from Brazil capable of causing GLS and ABR arose independently of those from Uruguay. Possible causes leading to the evolutionary differences between populations are discussed.
[Mh] Termos MeSH primário: Colletotrichum/genética
Malus/microbiologia
[Mh] Termos MeSH secundário: Colletotrichum/patogenicidade
Variação Genética
Fenótipo
Phyllachorales
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1609
[Cu] Atualização por classe:160614
[Lr] Data última revisão:
160614
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160329
[St] Status:MEDLINE
[do] DOI:10.1094/PHYTO-09-15-0222-R


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[PMID]:26976892
[Au] Autor:Cabada MM; Castellanos-Gonzalez A; Lopez M; Caravedo MA; Arque E; White AC
[Ad] Endereço:Universidad Peruana Cayetano Heredia and University of Texas Medical Branch Collaborative Research Center, Cusco, Peru; Infectious Diseases Division, Department of Internal Medicine, University of Texas Medical Branch, Galveston, Texas micabada@utmb.edu.
[Ti] Título:Fasciola hepatica Infection in an Indigenous Community of the Peruvian Jungle.
[So] Source:Am J Trop Med Hyg;94(6):1309-12, 2016 Jun 01.
[Is] ISSN:1476-1645
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Fasciola hepatica is a zoonotic infection with a worldwide distribution. Autochthonous cases have not been reported in the Amazon region of Peru. Operculated eggs resembling F. hepatica were identified in the stools of five out of 215 subjects in a remote indigenous community of the Peruvian jungle. Polymerase chain reaction targeting Fasciola hepatica cytochrome oxidase subunit 1 (COI) gene and sequencing of the products confirmed Fasciola infection.
[Mh] Termos MeSH primário: Fasciola hepatica/genética
Fasciolíase/epidemiologia
[Mh] Termos MeSH secundário: Adolescente
Animais
Criança
Complexo IV da Cadeia de Transporte de Elétrons/genética
Fasciola hepatica/enzimologia
Fezes/parasitologia
Feminino
Regulação Enzimológica da Expressão Gênica
Seres Humanos
Masculino
Peru/epidemiologia
Phyllachorales
Reação em Cadeia da Polimerase/métodos
Grupos Populacionais
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 1.9.3.1 (Electron Transport Complex IV)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170601
[Lr] Data última revisão:
170601
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:160316
[St] Status:MEDLINE
[do] DOI:10.4269/ajtmh.15-0769


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[PMID]:26881925
[Au] Autor:Wang S; Saito T; Ohkawa K; Ohara H; Shishido M; Ikeura H; Takagi K; Ogawa S; Yokoyama M; Kondo S
[Ad] Endereço:Graduate School of Horticulture, Chiba University, Matsudo 271-8510, Japan.
[Ti] Título:α-Ketol linolenic acid (KODA) application affects endogenous abscisic acid, jasmonic acid and aromatic volatiles in grapes infected by a pathogen (Glomerella cingulata).
[So] Source:J Plant Physiol;192:90-7, 2016 Mar 15.
[Is] ISSN:1618-1328
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Effects of α-ketol linolenic acid (KODA) application on endogenous abscisic acid (ABA), jasmonic acid (JA), and aromatic volatiles were investigated in 'Kyoho' grapes (Vitis labrusca×Vitis vinifera) infected by a pathogen (Glomerella cingulata). The expressions of 9-cis-epoxycarotenoid dioxygenase (VvNCED1), ABA 8'-hydroxylase (VvCYP707A1), lipoxygenase (VvLOX), and allene oxide synthase (VvAOS) were also examined. The grape berries were dipped in 0.1mM KODA solution before inoculation with the pathogen and stored at 25°C for 12 days. The development of infection was significantly suppressed upon KODA treatment. Endogenous ABA, JA and phaseic acid (PA) were induced in inoculated berries. KODA application before inoculation increased endogenous ABA, PA and JA through the activation of VvNCED1, VvCYP707A1 and VvAOS genes, respectively. In addition, terpenes, methyl salicylate (Me-SA) and C6-aldehydes such as (E)-2-hexenal and cis-3-hexenal associated with fungal resistance also increased in KODA-treated berries during storage. These results suggest that the synergistic effect of JA, ABA, and some aromatic volatiles induced by KODA application may provide resistance to pathogen infection in grape berries.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/metabolismo
Phyllachorales/fisiologia
Doenças das Plantas/microbiologia
Reguladores de Crescimento de Planta/metabolismo
Transdução de Sinais
Vitis/genética
[Mh] Termos MeSH secundário: Ácido Abscísico/metabolismo
Aldeídos/metabolismo
Antioxidantes/metabolismo
Proteínas de Arabidopsis/genética
Ciclopentanos/metabolismo
Sistema Enzimático do Citocromo P-450/genética
Sistema Enzimático do Citocromo P-450/metabolismo
Dioxigenases/genética
Dioxigenases/metabolismo
Frutas/genética
Frutas/imunologia
Frutas/microbiologia
Frutas/fisiologia
Oxirredutases Intramoleculares/genética
Oxirredutases Intramoleculares/metabolismo
Lipoxigenase/genética
Lipoxigenase/metabolismo
Oxilipinas/metabolismo
Doenças das Plantas/imunologia
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
Vitis/imunologia
Vitis/microbiologia
Vitis/fisiologia
Ácido alfa-Linolênico/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (3-hexenal); 0 (Aldehydes); 0 (Antioxidants); 0 (Arabidopsis Proteins); 0 (Cyclopentanes); 0 (Oxylipins); 0 (Plant Growth Regulators); 0 (Plant Proteins); 0RBV727H71 (alpha-Linolenic Acid); 505-57-7 (2-hexenal); 6RI5N05OWW (jasmonic acid); 72S9A8J5GW (Abscisic Acid); 9035-51-2 (Cytochrome P-450 Enzyme System); EC 1.13.11.- (Dioxygenases); EC 1.13.11.12 (Lipoxygenase); EC 1.13.11.51 (9-cis-epoxy-carotenoid dioxygenase); EC 1.14.13.93 (abscisic acid 8'-hydroxylase); EC 5.3.- (Intramolecular Oxidoreductases); EC 5.3.99.6 (hydroperoxide isomerase)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170828
[Lr] Data última revisão:
170828
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160217
[St] Status:MEDLINE


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[PMID]:26571694
[Au] Autor:Li Y; Zhao L; Cui L; Lei J; Zhang X
[Ti] Título:[Effects of elicitors on growth of adventitious roots and contents of secondary metabolites in Tripterygium wilfordii Hook. f].
[So] Source:Sheng Wu Gong Cheng Xue Bao;31(5):734-43, 2015 May.
[Is] ISSN:1000-3061
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:To study the effects of the extract of fungal elicitor, AgNO3, MeJA and yeast on the growth and content of secondary metabolites of adventitious roots in Tripterygium wilfordii. The above elicitors were supplemented to the medium, the growth and the content of secondary metabolites were measured. When the medium was supplemented with the elicitor Glomerella cingulata or Collectotrichum gloeosporioides, the content of triptolide was increased by 2.24 and 1.93-fold, the alkaloids content was increased by 2.02 and 2.07-fold, respectively. The optimal concentration of G. cingulata was 50 µg/mL for accumulation of triptolide, alkaloids and for the growth of adventitious roots. AgNO3 inhibited the growth of adventitious roots and the accumulation of the alkaloids, whereas it (at 25 µmol/L) increased the accumulation of triptolide by 1.71-fold compared to the control. The growth of adventitious roots, the contents of triptolide and alkaloids were increased 1.04, 1.64 and 2.12-folds, respectively when MeJA was at 50 µmol/L. When the concentration of yeast reached 2 g/L, the content of triptolide increased 1.48-folds. This research demonstrated that supplementation of AgNO3 and yeast enhanced the biosynthesis of triptolide in adventitious roots and the synergism of G. cingulata and MeJA could promote the biosynthesis of both triptolide and alkaloids.
[Mh] Termos MeSH primário: Alcaloides/biossíntese
Diterpenos/metabolismo
Fenantrenos/metabolismo
Raízes de Plantas/crescimento & desenvolvimento
Tripterygium/efeitos dos fármacos
[Mh] Termos MeSH secundário: Acetatos/farmacologia
Colletotrichum
Ciclopentanos/farmacologia
Medicamentos de Ervas Chinesas
Compostos de Epóxi/metabolismo
Oxilipinas/farmacologia
Phyllachorales
Raízes de Plantas/efeitos dos fármacos
Metabolismo Secundário
Tripterygium/crescimento & desenvolvimento
Tripterygium/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Acetates); 0 (Alkaloids); 0 (Cyclopentanes); 0 (Diterpenes); 0 (Drugs, Chinese Herbal); 0 (Epoxy Compounds); 0 (Oxylipins); 0 (Phenanthrenes); 19ALD1S53J (triptolide); 900N171A0F (methyl jasmonate)
[Em] Mês de entrada:1601
[Cu] Atualização por classe:161126
[Lr] Data última revisão:
161126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151118
[St] Status:MEDLINE


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[PMID]:26471091
[Au] Autor:Gao Z; Lv M; Li Q; Xu H
[Ad] Endereço:Research Institute of Pesticidal Design & Synthesis, College of Sciences/Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, People's Republic of China.
[Ti] Título:Synthesis of heterocycle-attached methylidenebenzenesulfonohydrazones as antifungal agents.
[So] Source:Bioorg Med Chem Lett;25(22):5092-6, 2015 Nov 15.
[Is] ISSN:1464-3405
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A series of heterocycle-attached methylidenebenzenesulfonohydrazone derivatives were synthesized and evaluated for their antifungal activities against seven phytopathogenic fungi such as Fusarium graminearum, Alternaria solani, Valsa mali, Phytophthora capsici, Fusarium solani, Botrytis cinerea, and Glomerella cingulata. Compounds 7b, 8d, 9a, 9b and 9d exhibited a good and broad-spectrum of antifungal activities against at least five phytopathogenic fungi at the concentration of 100 µg/mL. It demonstrated that addition of one double bond between the phenylsulfonylhydrazone fragment and the furan ring of 6a,b,d could afford more active compounds 9a,b,d; however, introduction of the nitro group on the phenyl ring of 6a-9a gave less potent compounds 6e-9e.
[Mh] Termos MeSH primário: Antifúngicos/farmacologia
Hidrazonas/farmacologia
Sulfonamidas/farmacologia
[Mh] Termos MeSH secundário: Alternaria/efeitos dos fármacos
Antifúngicos/síntese química
Botrytis/efeitos dos fármacos
Fusarium/efeitos dos fármacos
Hidrazonas/síntese química
Oxazóis/farmacologia
Phyllachorales/efeitos dos fármacos
Phytophthora/efeitos dos fármacos
Sulfonamidas/síntese química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Hydrazones); 0 (Oxazoles); 0 (Sulfonamides); 20T2M875LO (hymexazol)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:151031
[Lr] Data última revisão:
151031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151017
[St] Status:MEDLINE



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