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Svidzinski, Terezinha Inez Estivalet
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[PMID]:29262785
[Au] Autor:da Silva EM; Mansano ESB; Miazima ES; Rodrigues FAV; Hernandes L; Svidzinski TIE
[Ad] Endereço:Department of Medical Mycology, State University of Maringá, Av. Colombo, 5760, C.P, Maringá, PR, 87020-900, Brazil.
[Ti] Título:Radiation used for head and neck cancer increases virulence in Candida tropicalis isolated from a cancer patient.
[So] Source:BMC Infect Dis;17(1):783, 2017 Dec 20.
[Is] ISSN:1471-2334
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Studies have shown that radiation from radiotherapy increases the yeast colonization of patients. However it is not clear, if such radiation alters the yeast itself. The aim of the present study was therefore to report the direct impact of gamma radiation on Candida tropicalis. METHODS: C. tropicalis was obtained from a patient with a carcinoma, a suspension of this yeast containing 2.0 × 10 colony forming units per milliliter was prepared. It was submitted to gamma radiation dosage similar to that used in the treatment of head and neck cancer. After a cumulative dose of 7200 cGy some virulence attributes of C. tropicalis, including macro and micromorphological characteristics, adhesion and biofilm abilities, murine experimental infection and phagocytosis resistance were evaluated on irradiated and non-irradiated yeasts. RESULTS: After irradiation the colony morphology of the yeast was altered from a ring format to a smooth appearance in most colonies. Scanning electron microscopy revealed notable differences in the structures of both these colonies and the yeast cells, with the loss of pseudohyphae following irradiation and an increase in extracellular matrix production. The adherence and biofilm production of the yeast was greater following irradiation, both in terms of the number of yeasts and total biomass production on several abiotic surfaces and TR146 cells. The phagocytic index of the irradiated yeasts was not statistically different; however, the presence of cellular debris was detected in the kidneys of infected animals. Mice infected with irradiated yeasts developed an infection at the site of the yeast inoculation, although systemic infection was unchanged. CONCLUSIONS: Our findings show for the first time that C. tropicalis, one of the most important yeasts from colonization, which cause fatal candidemia in cancer patients, is affected by gamma irradiation, with changes to its virulence profile.
[Mh] Termos MeSH primário: Candida tropicalis
Candidíase Invasiva
Neoplasias de Cabeça e Pescoço
Radioterapia/efeitos adversos
Virulência/efeitos da radiação
[Mh] Termos MeSH secundário: Biofilmes
Candida tropicalis/patogenicidade
Candida tropicalis/efeitos da radiação
Neoplasias de Cabeça e Pescoço/complicações
Neoplasias de Cabeça e Pescoço/radioterapia
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171222
[St] Status:MEDLINE
[do] DOI:10.1186/s12879-017-2879-6


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[PMID]:29390542
[Au] Autor:Murgu AM; Criscov IG; Fotea S; Baciu G; Chiriac A; Tarca E; Streanga V
[Ad] Endereço:University of Medicine and Pharmacy "Gr. T. Popa".
[Ti] Título:Particularities of the management and the treatment in a rare sepsis with Candida tropicalis of a Collodion baby: Case report.
[So] Source:Medicine (Baltimore);96(51):e9387, 2017 Dec.
[Is] ISSN:1536-5964
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:RATIONALE: Collodion baby is a rare autosomal recessive disorder. It can be the first expression of some forms of ichthyosis. PATIENT CONCERNS: The authors present the case of a newborn diagnosed with severe Collodion baby syndrome who required prolonged hospitalization in the intensive care unit because of infectious complications like the fungal sepsis and other bacterial superinfections. DIAGNOSES: The case has many diagnostic and therapeutic particularities and management difficulties. Skin culture, dermatological and genetic exam were required. INTERVENTIONS: The treatment required multidisciplinary involvement: neonatologist, pediatrician, geneticist, dermatologist, psychologist, ophthalmologist, audiologist. OUTCOMES: The evolution during hospitalization was slowly favorable, but later, after a few months, it developed some complications. LESSONS: In our case, skin injuries, total parenteral nutrition, aggressive and prolonged antibiotic therapy, intravenous devices, high hospitalization duration were risk factors for colonization and sepsis with fungi, especially in the neonatal period, sometimes with severe evolution and prognosis.
[Mh] Termos MeSH primário: Candida tropicalis
Candidíase/terapia
Ictiose Lamelar/complicações
Sepse/terapia
[Mh] Termos MeSH secundário: Candidíase/diagnóstico
Candidíase/etiologia
Seres Humanos
Ictiose Lamelar/diagnóstico
Recém-Nascido
Masculino
Sepse/diagnóstico
Sepse/etiologia
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:180203
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000009387


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[PMID]:28854190
[Au] Autor:Al-Obaid K; Asadzadeh M; Ahmad S; Khan Z
[Ad] Endereço:Microbiology, Department of Medical Laboratories, Al-Amiri Hospital, Sharq, Kuwait.
[Ti] Título:Population structure and molecular genetic characterization of clinical Candida tropicalis isolates from a tertiary-care hospital in Kuwait reveal infections with unique strains.
[So] Source:PLoS One;12(8):e0182292, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Candida tropicalis is a frequently isolated yeast species causing bloodstream, urinary tract and other infections particularly in patients admitted to intensive care units (ICUs) and those requiring prolonged urinary catheterization (UC) or receiving broad-spectrum antibiotics (BSA). This study investigated clinical characteristics and genetic relatedness among C. tropicalis strains isolated from patients at Al-Amiri Hospital in Kuwait. C. tropicalis strains (n = 63) isolated from blood, genito-urinary, respiratory (RT) and digestive (GIT) tracts and wound sites from 54 patients were used. All isolates were phenotypically identified and tested against six antifungal drugs by using Vitek 2 system. Molecular identification was performed by PCR amplification of rDNA. Fingerprinting was achieved by 6-loci-based multilocus sequence typing (MLST) and data were analyzed by BioNumerics software for phylogenetic relationships. Patients mean age was >65 years and >20% patients were hospitalized in ICUs. Most patients had underlying conditions that included UC, BSA, diabetes and RT/GIT abnormalities. Most candiduria cases had UC, ureteric stent or suprapubic catheters. All isolates were identified as C. tropicalis by Vitek 2 and by species-specific PCR. Sixty-two isolates were susceptible to all tested antifungal drugs. MLST identified 59 diploid sequence types (DSTs) including 54 newly-identified DSTs. C. tropicalis isolates from multiple sites of same patient usually belonged to different DSTs. Interestingly, 56 of 57 isolates from 48 patients belonged to unique genotypes. Only six isolates from six patients belonged to three DSTs (clusters), however, C. tropicalis strains in each cluster were isolated >3 months apart. Our data show diverse origins of C. tropicalis infections in Kuwait as most isolates were unique strains. There was no obvious correlation between cluster isolates with time of isolation and/or hospital ward of their origin. This study presents the first MLST analysis of C. tropicalis isolates from Middle East and may be useful for studying genetic relationships among global C. tropicalis strains.
[Mh] Termos MeSH primário: Candida tropicalis/genética
Candidíase/microbiologia
[Mh] Termos MeSH secundário: Antifúngicos/farmacologia
Candida tropicalis/classificação
Candida tropicalis/efeitos dos fármacos
Candida tropicalis/isolamento & purificação
Candidíase/diagnóstico
Candidíase/tratamento farmacológico
Candidíase/epidemiologia
Farmacorresistência Fúngica
Variação Genética
Genótipo
Seres Humanos
Kuweit/epidemiologia
Testes de Sensibilidade Microbiana
Tipagem de Sequências Multilocus
Técnicas de Tipagem Micológica
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170831
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182292


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[PMID]:28723923
[Au] Autor:Marioni J; Bresolí-Obach R; Agut M; Comini LR; Cabrera JL; Paraje MG; Nonell S; Núñez Montoya SC
[Ad] Endereço:IMBIV, CONICET and Departamento de Ciencias Farmacéuticas, Facultad de Ciencias Químicas, Universidad Nacional Córdoba, Córdoba, Argentina.
[Ti] Título:On the mechanism of Candida tropicalis biofilm reduction by the combined action of naturally-occurring anthraquinones and blue light.
[So] Source:PLoS One;12(7):e0181517, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The photoprocesses involved in the photo-induced Candida tropicalis biofilm reduction by two natural anthraquinones (AQs), rubiadin (1) and rubiadin-1-methyl ether (2), were examined. Production of singlet oxygen (1O2) and of superoxide radical anion (O2•-) was studied. Although it was not possible to detect the triplet state absorption of any AQs in biofilms, observation of 1O2 phosphorescence incubated with deuterated Phosphate Buffer Solution, indicated that this species is actually formed in biofilms. 2 was accumulated in the biofilm to a greater extent than 1 and produced measurable amounts of O2•- after 3h incubation in biofilms. The effect of reactive oxygen species scavengers on the photo-induced biofilm reduction showed that Tiron (a specific O2•- scavenger) is most effective than sodium azide (a specific 1O2 quencher). This suggests that O2•- formed by electron transfer quenching of the AQs excited states, is the main photosensitizing mechanism involved in the photo-induced antibiofilm activity, whereas 1O2 participation seems of lesser importance.
[Mh] Termos MeSH primário: Antraquinonas/farmacologia
Biofilmes/efeitos dos fármacos
Candida tropicalis/efeitos dos fármacos
Luz
[Mh] Termos MeSH secundário: Biofilmes/crescimento & desenvolvimento
Candida tropicalis/metabolismo
Espécies Reativas de Oxigênio
Superóxidos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthraquinones); 0 (Reactive Oxygen Species); 11062-77-4 (Superoxides); 7460-43-7 (rubiadin 1-methyl ether); CY0UH3X06R (rubiadin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170721
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0181517


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Texto completo SciELO Chile
[PMID]:28632834
[Au] Autor:Santos E; Mercado M; Luévanos A; Martínez P; Guerrero M
[Ad] Endereço:Hospital Civil de Guadalajara Fray Antonio Alcalde, México.
[Ti] Título:[Bladder fungus ball by Candida tropicalis: a pediatric case report].
[Ti] Título:Fungoma vesical por Candida tropicalis: un caso clínico pediátrico..
[So] Source:Rev Chilena Infectol;34(2):186-189, 2017 Apr.
[Is] ISSN:0717-6341
[Cp] País de publicação:Chile
[La] Idioma:spa
[Ab] Resumo:Vesical fungus ball is a mobile, oval and echogenic mass as a result of accumulation of long and wide numerous hyphae. Fungal urinary tract infection incidence has increased notoriously and there are isolated yeast in 7 to 8% of urine cultures. Different species of Candida are cause of urinary tract infection. Epidemiologically, the first isolated pathogen is Candida albicans, followed by Candida tropicalis. Bladder poll has been documented as the most important risk factor for candiduria in critical patients into intensive care.
[Mh] Termos MeSH primário: Candida tropicalis/isolamento & purificação
Candidíase/microbiologia
Infecção Hospitalar/microbiologia
Doenças da Bexiga Urinária/microbiologia
[Mh] Termos MeSH secundário: Pré-Escolar
Seres Humanos
Masculino
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170726
[Lr] Data última revisão:
170726
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170621
[St] Status:MEDLINE


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[PMID]:28504678
[Au] Autor:Rosenthal RG; Vögeli B; Wagner T; Shima S; Erb TJ
[Ad] Endereço:Biochemistry and Synthetic Biology of Microbial Metabolism Group, Max Planck Institute for Terrestrial Microbiology, Marburg, Germany.
[Ti] Título:A conserved threonine prevents self-intoxication of enoyl-thioester reductases.
[So] Source:Nat Chem Biol;13(7):745-749, 2017 Jul.
[Is] ISSN:1552-4469
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Enzymes are highly specific biocatalysts, yet they can promote unwanted side reactions. Here we investigated the factors that direct catalysis in the enoyl-thioester reductase Etr1p. We show that a single conserved threonine is essential to suppress the formation of a side product that would otherwise act as a high-affinity inhibitor of the enzyme. Substitution of this threonine with isosteric valine increases side-product formation by more than six orders of magnitude, while decreasing turnover frequency by only one order of magnitude. Our results show that the promotion of wanted reactions and the suppression of unwanted side reactions operate independently at the active site of Etr1p, and that the active suppression of side reactions is highly conserved in the family of medium-chain dehydrogenases/reductases (MDRs). Our discovery emphasizes the fact that the active destabilization of competing transition states is an important factor during catalysis that has implications for the understanding and the de novo design of enzymes.
[Mh] Termos MeSH primário: Oxirredutases atuantes sobre Doadores de Grupo CH-CH/antagonistas & inibidores
Treonina/farmacologia
[Mh] Termos MeSH secundário: Biocatálise
Candida tropicalis/enzimologia
Relação Dose-Resposta a Droga
Mitocôndrias/enzimologia
Estrutura Molecular
Oxirredutases atuantes sobre Doadores de Grupo CH-CH/metabolismo
Relação Estrutura-Atividade
Treonina/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
2ZD004190S (Threonine); EC 1.3.- (Oxidoreductases Acting on CH-CH Group Donors); EC 1.3.1.38 (trans-2-enoyl-CoA reductase (NADPH))
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170516
[St] Status:MEDLINE
[do] DOI:10.1038/nchembio.2375


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[PMID]:28364558
[Au] Autor:Chapman B; Slavin M; Marriott D; Halliday C; Kidd S; Arthur I; Bak N; Heath CH; Kennedy K; Morrissey CO; Sorrell TC; van Hal S; Keighley C; Goeman E; Underwood N; Hajkowicz K; Hofmeyr A; Leung M; Macesic N; Botes J; Blyth C; Cooley L; George CR; Kalukottege P; Kesson A; McMullan B; Baird R; Robson J; Korman TM; Pendle S; Weeks K; Liu E; Cheong E; Chen S; Australian and New Zealand Mycoses Interest Group
[Ad] Endereço:Westmead Institute for Medical Research, The University of Sydney, Sydney, NSW, Australia.
[Ti] Título:Changing epidemiology of candidaemia in Australia.
[So] Source:J Antimicrob Chemother;72(4):1103-1108, 2017 Apr 01.
[Is] ISSN:1460-2091
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Objectives: Knowledge of contemporary epidemiology of candidaemia is essential. We aimed to identify changes since 2004 in incidence, species epidemiology and antifungal susceptibilities of Candida spp. causing candidaemia in Australia. Methods: These data were collected from nationwide active laboratory-based surveillance for candidaemia over 1 year (within 2014-2015). Isolate identification was by MALDI-TOF MS supplemented by DNA sequencing. Antifungal susceptibility testing was performed using Sensititre YeastOne™. Results: A total of 527 candidaemia episodes (yielding 548 isolates) were evaluable. The mean annual incidence was 2.41/105 population. The median patient age was 63 years (56% of cases occurred in males). Of 498 isolates with confirmed species identity, Candida albicans was the most common (44.4%) followed by Candida glabrata complex (26.7%) and Candida parapsilosis complex (16.5%). Uncommon Candida species comprised 25 (5%) isolates. Overall, C. albicans (>99%) and C. parapsilosis (98.8%) were fluconazole susceptible. However, 16.7% (4 of 24) of Candida tropicalis were fluconazole- and voriconazole-resistant and were non-WT to posaconazole. Of C. glabrata isolates, 6.8% were resistant/non-WT to azoles; only one isolate was classed as resistant to caspofungin (MIC of 0.5 mg/L) by CLSI criteria, but was micafungin and anidulafungin susceptible. There was no azole/echinocandin co-resistance. Conclusions: We report an almost 1.7-fold proportional increase in C. glabrata candidaemia (26.7% versus 16% in 2004) in Australia. Antifungal resistance was generally uncommon, but azole resistance (16.7% of isolates) amongst C. tropicalis may be emerging.
[Mh] Termos MeSH primário: Antifúngicos/farmacologia
Candida/efeitos dos fármacos
Candida/isolamento & purificação
Candidemia/epidemiologia
Candidemia/microbiologia
[Mh] Termos MeSH secundário: Austrália/epidemiologia
Azóis/farmacologia
Candida/classificação
Candida/genética
Candida glabrata/efeitos dos fármacos
Candida glabrata/genética
Candida glabrata/isolamento & purificação
Candida tropicalis/efeitos dos fármacos
Candida tropicalis/genética
Candida tropicalis/isolamento & purificação
Farmacorresistência Fúngica/genética
Equinocandinas/farmacologia
Feminino
Fluconazol/farmacologia
Seres Humanos
Incidência
Lipopeptídeos/farmacologia
Masculino
Testes de Sensibilidade Microbiana/métodos
Análise de Sequência de DNA/métodos
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
Triazóis/farmacologia
Voriconazol/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Azoles); 0 (Echinocandins); 0 (Lipopeptides); 0 (Triazoles); 6TK1G07BHZ (posaconazole); 8VZV102JFY (Fluconazole); 9HLM53094I (anidulafungin); F0XDI6ZL63 (caspofungin); JFU09I87TR (Voriconazole); R10H71BSWG (micafungin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170402
[St] Status:MEDLINE
[do] DOI:10.1093/jac/dkw422


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[PMID]:28260588
[Au] Autor:Khodadadi H; Karimi L; Jalalizand N; Adin H; Mirhendi H
[Ad] Endereço:1​Department of Medical Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
[Ti] Título:Utilization of size polymorphism in ITS1 and ITS2 regions for identification of pathogenic yeast species.
[So] Source:J Med Microbiol;66(2):126-133, 2017 Mar.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: Despite the existence of a variety of available yeast-identification strategies, easier and more cost-effective methods are required for routine use in clinical laboratories. The internal transcribed spacer (ITS) regions of fungal rRNA genes exhibit variable sizes depending on the yeast species. In the present study, fragment size polymorphism (FSP) analysis of the ITS1 and ITS2 regions for identification of the clinically most important yeast species was assessed. METHODOLOGY: The ITS1 and ITS2 regions of 190 strains, including isolates of 31 standard strains and 159 clinical isolates, were separately PCR amplified with two primer sets: ITS1-ITS2 and ITS3-ITS4. PCR products were mixed and the two-band electrophoretic pattern of each sample was analysed according to the size of the ITS regions as predicted from the GenBank database. RESULTS: Using this method and avoiding expensive tools such as sequencing or capillary electrophoresis, we were able to differentiate nearly all pathogenic yeast species, including Candida albicans, Candida tropicalis, Candida glabrata, Candida parapsilosis, Candida krusei, Candida guilliermondii, Candida kefyr, Candida lusitaniae, Candida rugosa, Cryptococcus neoformans and Saccharomyces cerevisiae. The method showed limited discriminatory power to differentiate species of the Candida parapsilosis complex. Differentiation of Candida albicans and Candida tropicalis needs already identified controls. CONCLUSION: FSP method benefits from advantages such as lower cost, higher speed and wider range of species than some commercial yeast-identification methods. We consider this method as one of the easiest molecular approaches for identifying a wide range of human pathogenic yeast species, applicable to both diagnostic and epidemiological purposes.
[Mh] Termos MeSH primário: Candida albicans/genética
Candida/genética
Cryptococcus neoformans/genética
DNA Espaçador Ribossômico/genética
Saccharomyces cerevisiae/genética
[Mh] Termos MeSH secundário: Candida/classificação
Candida glabrata/genética
Candida tropicalis/genética
DNA Fúngico/genética
Bases de Dados Genéticas
Genes de RNAr
Polimorfismo Genético
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Fungal); 0 (DNA, Ribosomal Spacer)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170321
[Lr] Data última revisão:
170321
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170307
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000426


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[PMID]:28254443
[Au] Autor:França EJ; Furlaneto-Maia L; Furlaneto MC
[Ad] Endereço:Department of Microbiology, State University of Londrina, Paraná, Brazil; State University of North of Paraná, Brazil.
[Ti] Título:Hemolytic capability and expression of a putative haem oxygenase-encoding gene by blood isolates of Candida tropicalis are influenced by iron deprivation and the presence of hemoglobin and erythrocytes.
[So] Source:Microb Pathog;105:235-239, 2017 Apr.
[Is] ISSN:1096-1208
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Although hemolytic activity is known to be a putative virulence factor contributing to candidal pathogenesis, its production by Candida tropicalis, a species closely related to Candida albicans, is poor understood. The present study was undertaken to evaluate the hemolytic activity and the expression level of a putative haem oxygenase encoding gene by blood isolates of C. tropicalis following growth in iron deprivation, and in the presence of hemoglobin and erythrocytes. The lowest values of hemolytic activity were observed in cell-free culture supernatants of isolates growing in iron-restricted medium (RPMI medium and RPMI medium supplemented with iron chelator bathophenanthrolindisulphonic acid). Hemolysis was increased in the presence of either hemoglobin or erythrocytes. Reverse transcriptase PCR analysis showed that the putative haem oxygenase encoding gene (CtHMX1), potentially related with iron uptake, was up-regulated (p < 0.001) following growth in iron deprivation and in the presence of hemoglobin; CtHMX1 was repressed in the presence of human erythrocytes (p < 0.001). Our data suggest that hemoglobin had positive effect in the production of hemolytic factor and gene expression related to iron uptake in C. tropicalis.
[Mh] Termos MeSH primário: Sangue/microbiologia
Candida tropicalis/enzimologia
Candida tropicalis/genética
Eritrócitos/metabolismo
Heme Oxigenase (Desciclizante)/genética
Heme Oxigenase (Desciclizante)/metabolismo
Hemoglobinas/metabolismo
Ferro/metabolismo
[Mh] Termos MeSH secundário: Candida tropicalis/crescimento & desenvolvimento
Candida tropicalis/ultraestrutura
Candidíase/sangue
Candidíase/microbiologia
Meios de Cultura
DNA Fúngico/isolamento & purificação
Proteínas Fúngicas/biossíntese
Proteínas Fúngicas/genética
Fungos/crescimento & desenvolvimento
Proteínas Hemolisinas
Hemólise
Seres Humanos
RNA Fúngico/isolamento & purificação
Regulação para Cima
Fatores de Virulência/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Culture Media); 0 (DNA, Fungal); 0 (Fungal Proteins); 0 (Hemoglobins); 0 (Hemolysin Proteins); 0 (RNA, Fungal); 0 (Virulence Factors); E1UOL152H7 (Iron); EC 1.14.14.18 (Heme Oxygenase (Decyclizing))
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170505
[Lr] Data última revisão:
170505
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170304
[St] Status:MEDLINE


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[PMID]:28152096
[Au] Autor:Kim DJ; Lee MW; Choi JS; Lee SG; Park JY; Kim SW
[Ad] Endereço:Department of Integrated Biomedical and Life Sciences, Graduate School, Korea University, Seoul, Korea.
[Ti] Título:Inhibitory activity of hinokitiol against biofilm formation in fluconazole-resistant Candida species.
[So] Source:PLoS One;12(2):e0171244, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to investigate the ability of hinokitiol to inhibit the formation of Candida biofilms. Biofilm inhibition was evaluated by quantification of the biofilm metabolic activity with XTT assay. Hinokitiol efficiently prevented biofilm formation in both fluconazole-susceptible and fluconazole-resistant strains of Candida species. We determined the expression levels of specific genes previously implicated in biofilm development of C. albicans cells by real-time RT-PCR. The expression levels of genes associated with adhesion process, HWP1 and ALS3, were downregulated by hinokitiol. Transcript levels of UME6 and HGC1, responsible for long-term hyphal maintenance, were also decreased by hinokitiol. The expression level of CYR1, which encodes the component of signaling pathway of hyphal formation-cAMP-PKA was suppressed by hinokitiol. Its upstream general regulator RAS1 was also suppressed by hinokitiol. These results indicate that hinokitiol may have therapeutic potential in the treatment and prevention of biofilm-associated Candida infections.
[Mh] Termos MeSH primário: Antifúngicos/farmacologia
Biofilmes/efeitos dos fármacos
Candida/efeitos dos fármacos
Monoterpenos/farmacologia
Tropolona/análogos & derivados
[Mh] Termos MeSH secundário: Candida albicans/efeitos dos fármacos
Candida glabrata/efeitos dos fármacos
Candida tropicalis/efeitos dos fármacos
Farmacorresistência Fúngica
Fluconazol/farmacologia
Regulação Fúngica da Expressão Gênica/efeitos dos fármacos
Testes de Sensibilidade Microbiana
Reação em Cadeia da Polimerase em Tempo Real
Tropolona/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Monoterpenes); 7L6DL16P1T (Tropolone); 8VZV102JFY (Fluconazole); U5335D6EBI (beta-thujaplicin)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170203
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0171244



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