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Pesquisa : B01.300.179.100.650 [Categoria DeCS]
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[PMID]:28746729
[Au] Autor:Musa SF; Yeat TS; Kamal LZM; Tabana YM; Ahmed MA; El Ouweini A; Lim V; Keong LC; Sandai D
[Ad] Endereço:Infectomics Cluster, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Penang, Malaysia.
[Ti] Título:Pleurotus sajor-caju can be used to synthesize silver nanoparticles with antifungal activity against Candida albicans.
[So] Source:J Sci Food Agric;98(3):1197-1207, 2018 Feb.
[Is] ISSN:1097-0010
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Green synthesis of silver nanoparticles (AgNPs) has become widely practiced worldwide. In this study, AgNPs were synthesized using a hot-water extract of the edible mushroom Pleurotus sajor-caju. The product, PSC-AgNPs, was characterized by using UV-visible spectra, dynamic light scattering analysis, transmission electron microscopy (TEM), X-ray diffraction (XRD) and Fourier transform infrared (FTIR) spectrometry. To assess its antifungal activity against Candida albicans, gene transcription and protein expression analyses were conducted for CaICL1 and its product, ICL, using real-time quantitative polymerase chain reaction and western blot, respectively. RESULTS: PSC-AgNPs with an average particle size of 11.68 nm inhibited the growth of the pathogenic yeast C. albicans. Values for minimum inhibitory concentration and minimum fungicidal concentration were 250 and 500 mg L , respectively. TEM images revealed that the average particle size of PSC-AgNPs was 16.8 nm, with the values for zeta potential and the polydispersity index being -8.54 mV and 0.137, respectively. XRD and FTIR spectra showed PSC-AgNPs to have a face-centered cubic crystalline structure. The polysaccharides and amino acid residues present in P. sajor-caju extract were found to be involved in reducing Ag to AgNP. Both CaICL1 transcription and ICL protein expression were found to be suppressed in the cells treated with PSC-AgNPs as compared with the control. CONCLUSION: Our PSC-AgNP preparation makes for a promising antifungal agent that can downregulate isocitrate lyase. © 2017 Society of Chemical Industry.
[Mh] Termos MeSH primário: Antifúngicos/metabolismo
Antifúngicos/farmacologia
Candida albicans/efeitos dos fármacos
Pleurotus/metabolismo
Prata/metabolismo
Prata/farmacologia
[Mh] Termos MeSH secundário: Antifúngicos/química
Candida albicans/crescimento & desenvolvimento
Química Verde
Nanopartículas Metálicas/química
Testes de Sensibilidade Microbiana
Microscopia Eletrônica de Transmissão
Tamanho da Partícula
Extratos Vegetais/química
Extratos Vegetais/metabolismo
Pleurotus/química
Prata/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Plant Extracts); 3M4G523W1G (Silver)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180201
[Lr] Data última revisão:
180201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE
[do] DOI:10.1002/jsfa.8573


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[PMID]:28945798
[Au] Autor:Piscitelli A; Tarallo V; Guarino L; Sannia G; Birolo L; Pezzella C
[Ad] Endereço:Dipartimento di Scienze Chimiche, Università degli Studi di Napoli Federico II, Napoli, Italy.
[Ti] Título:New lipases by mining of Pleurotus ostreatus genome.
[So] Source:PLoS One;12(9):e0185377, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The analysis of Pleurotus ostreatus genome reveals the presence of automatically annotated 53 lipase and 34 carboxylesterase putative coding-genes. Since no biochemical or physiological data are available so far, a functional approach was applied to identify lipases from P. ostreatus. In the tested growth conditions, four lipases were found expressed, with different patterns depending on the used C source. Two of the four identified proteins (PleoLip241 and PleoLip369), expressed in both analysed conditions, were chosen for further studies, such as an in silico analysis and their molecular characterization. To overcome limits linked to native production, a recombinant expression approach in the yeast Pichia pastoris was applied. Different expression levels were obtained: PleoLip241 reached a maximum activity of 4000 U/L, whereas PleoLip369 reached a maximum activity of 700 U/L. Despite their sequence similarity, these enzymes exhibited different substrate specificity and diverse stability at pH, temperature, and presence of metals, detergents and organic solvents. The obtained data allowed classifying PleoLip241 as belonging to the "true lipase" family. Indeed, by phylogenetic analysis the two proteins fall in different clusters. PleoLip241 was used to remove the hydrophobic layer from wool surface in order to improve its dyeability. The encouraging results obtained with lipase treated wool led to forecast PleoLip241 applicability in this field.
[Mh] Termos MeSH primário: Proteínas Fúngicas/genética
Genoma Fúngico
Lipase/genética
Pleurotus/enzimologia
Pleurotus/genética
[Mh] Termos MeSH secundário: Animais
Carboxilesterase/química
Carboxilesterase/genética
Carboxilesterase/metabolismo
Domínio Catalítico
Corantes
Mineração de Dados
Proteínas Fúngicas/química
Proteínas Fúngicas/metabolismo
Microbiologia Industrial
Cinética
Lipase/química
Lipase/metabolismo
Modelos Moleculares
Filogenia
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Especificidade por Substrato

[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Coloring Agents); 0 (Fungal Proteins); 0 (Recombinant Proteins); EC 3.1.1.1 (Carboxylesterase); EC 3.1.1.3 (Lipase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170926
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185377


  3 / 1240 MEDLINE  
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[PMID]:28806986
[Au] Autor:Zhang C; Li J; Wang J; Song X; Zhang J; Wu S; Hu C; Gong Z; Jia L
[Ad] Endereço:Institute of Agricultural Resources and Environment, Shandong Academy of Agricultural Science, Key Laboratory of Wastes Matrix Utilization, Ministry of Agriculture, Jinan, 250100, China.
[Ti] Título:Antihyperlipidaemic and hepatoprotective activities of acidic and enzymatic hydrolysis exopolysaccharides from Pleurotus eryngii SI-04.
[So] Source:BMC Complement Altern Med;17(1):403, 2017 Aug 14.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Hyperlipidaemia is the major risk factor contributing to the development and progression of atherosclerosis, fatty liver and cerebrovascular disease. Pleurotus eryngii (P. eryngii) is rich in biologically active components, especially polysaccharides that exhibit various biological activities, including reducing blood lipids. In the present study, three novel polysaccharide types, including exopolysaccharides (EPS), enzymatic EPS (EEPS) and acidic EPS (AEPS) were isolated, and the hypolipidaemic and hepatoprotective effects were investigated to better understand possible hypolipidaemic mechanisms and their hepatoprotective effects. METHODS: The EPS was hydrolysed by snailase (dissolved in 1% acetic acid, pH = 6) and H SO (1 M) to obtain EEPS and AEPS, respectively. The in vitro antioxidant activities were measured by investigating the reducing power and the scavenging effects on radicals of hydroxyl, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and superoxide anion. The hyperlipidaemic mice were induced by perfusing a high-fat emulsion. In addition to the hepatic histopathology, the following biochemical analyses were performed to investigate the antioxidative effects, including the activities of alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and catalase (CAT). Triacylglycerol (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA) and lipid peroxidation (LPO) levels were also measured in serum and liver homogenate. RESULTS: Supplementation of EPS, EEPS and AEPS could significantly improve blood lipid levels (TC, TG, HDL-C, and LDL-C), hepatic lipid levels (TC and TG), hepatic enzyme activities (ALP, ALT, and AST) and antioxidant status (GSH-Px, SOD, T-AOC, MDA, and LPO). In addition, histopathological observations indicated that these polysaccharides had potential effects in attenuating hepatocyte damage. CONCLUSION: These results demonstrated that both EPS and its hydrolysates EEPS and AEPS might effectively reduce serum lipid levels and protect against high-fat diet-induced hyperlipidaemia, indicating that they could be used as functional foods and natural hepatoprotectants.
[Mh] Termos MeSH primário: Antioxidantes/farmacologia
Hiperlipidemias
Hipolipemiantes/farmacologia
Lipídeos/sangue
Fígado/efeitos dos fármacos
Pleurotus/química
Polissacarídeos/farmacologia
[Mh] Termos MeSH secundário: Animais
Antioxidantes/isolamento & purificação
Antioxidantes/metabolismo
Antioxidantes/uso terapêutico
Aspartato Aminotransferases
Produtos Biológicos/química
Produtos Biológicos/farmacologia
Produtos Biológicos/uso terapêutico
Compostos de Bifenilo/metabolismo
Catalase/metabolismo
Dieta Hiperlipídica
Glutationa Peroxidase/metabolismo
Hidrólise
Hiperlipidemias/sangue
Hiperlipidemias/tratamento farmacológico
Hiperlipidemias/etiologia
Hiperlipidemias/metabolismo
Hipolipemiantes/isolamento & purificação
Hipolipemiantes/uso terapêutico
Peroxidação de Lipídeos
Fígado/enzimologia
Fígado/metabolismo
Fígado/patologia
Malondialdeído
Camundongos
Estresse Oxidativo/efeitos dos fármacos
Fitoterapia
Picratos/metabolismo
Polissacarídeos/isolamento & purificação
Superóxido Dismutase/metabolismo
Superóxidos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Biological Products); 0 (Biphenyl Compounds); 0 (Hypolipidemic Agents); 0 (Lipids); 0 (Picrates); 0 (Polysaccharides); 11062-77-4 (Superoxides); 4Y8F71G49Q (Malondialdehyde); DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl); EC 1.11.1.6 (Catalase); EC 1.11.1.9 (Glutathione Peroxidase); EC 1.15.1.1 (Superoxide Dismutase); EC 2.6.1.1 (Aspartate Aminotransferases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170901
[Lr] Data última revisão:
170901
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170816
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-017-1892-z


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[PMID]:28780270
[Au] Autor:Naresh Kumar M; Ravikumar R; Thenmozhi S; Kirupa Sankar M
[Ad] Endereço:Bioenergy Research Laboratory, Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam, Erode 638401, TN, India.
[Ti] Título:Development of natural cellulase inhibitor mediated intensified biological pretreatment technology using Pleurotus florida for maximum recovery of cellulose from paddy straw under solid state condition.
[So] Source:Bioresour Technol;244(Pt 1):353-361, 2017 Nov.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Inhibitor mediated intensified bio-pretreatment (IMBP) technology using natural cellulase inhibitor (NCI) for maximum cellulose recovery from paddy straw was studied. Pretreatment was carried out under solid state condition. Supplementation of 8% NCI in pretreatment medium improves cellulose recovery and delignification by 1.2 and 1.5-fold respectively, compared to conventional bio-pretreatment due to inhibition of 61% of cellulase activity in IMBP. Further increase in NCI concentration showed negative effect on Pleurotus florida growth and suppress the laccase productivity by 1.1-fold. Laccase activity in IMBP was found to be 2.0U/mL on 19 day, which is higher than (1.5U/mL) conventional bio-pretreatment. Physico-chemical modifications in paddy straw before and after pretreatment were analysed by SEM, ATR-FTIR, XRD and TGA. According to these findings, the IMBP technology can be a viable eco-friendly technology for sustainable production of bioethanol with maximum cellulose recovery.
[Mh] Termos MeSH primário: Celulase
Celulose
Pleurotus
[Mh] Termos MeSH secundário: Lacase
Lignina
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9004-34-6 (Cellulose); 9005-53-2 (Lignin); EC 1.10.3.2 (Laccase); EC 3.2.1.4 (Cellulase)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170807
[St] Status:MEDLINE


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[PMID]:28771640
[Au] Autor:Branà MT; Cimmarusti MT; Haidukowski M; Logrieco AF; Altomare C
[Ad] Endereço:Institute of Sciences of Food Production, National Research Council of Italy, Bari, Italy.
[Ti] Título:Bioremediation of aflatoxin B1-contaminated maize by king oyster mushroom (Pleurotus eryngii).
[So] Source:PLoS One;12(8):e0182574, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Aflatoxin B1 (AFB1) is the most harmful mycotoxin that occurs as natural contaminant of agricultural commodities, particularly maize. Practical solutions for detoxification of contaminated staples and reduction of agricultural wastes are scarce. We investigated the capability of the white-rot and edible fungus Plerotus eryngii (king oyster mushroom) to degrade AFB1 both in vitro and in a laboratory-scale mushroom cultivation, using a substrate similar to that routinely used in mushroom farms. In malt extract broth, degradation of AFB1 (500 ng/mL) by nine isolates of P. eryngii ranged from 81 to 99% after 10 days growth, and reached 100% for all isolates after 30 days. The growth of P. eryngii on solid medium (malt extract-agar, MEA) was significantly reduced at concentrations of AFB1 500 ng/mL or higher. However, the addition of 5% wheat straw to the culture medium increased the tolerance of P. eryngii to AFB1 and no inhibition was observed at a AFB1 content of 500 ng/mL; degradation of AFB1 in MEA supplemented with 5% wheat straw and 2.5% (w/v) maize flour was 71-94% after 30 days of growth. Further, AFB1 degradation by P. eryngii strain ITEM 13681 was tested in a laboratory-scale mushroom cultivation. The mushroom growth medium contained 25% (w/w) of maize spiked with AFB1 to the final content of 128 µg/kg. Pleurotus eryngii degraded up to 86% of the AFB1 in 28 days, with no significant reduction of either biological efficiency or mushroom yield. Neither the biomass produced on the mushroom substrate nor the mature basidiocarps contained detectable levels of AFB1 or its metabolite aflatoxicol, thus ruling out the translocation of these toxins through the fungal thallus. These findings make a contribution towards the development of a novel technology for remediation of AFB1- contaminated corn through the exploitation of the degradative capability of P. eryngii and its bioconversion into high nutritional value material intended for feed production.
[Mh] Termos MeSH primário: Aflatoxina B1/metabolismo
Pleurotus/crescimento & desenvolvimento
Zea mays/microbiologia
[Mh] Termos MeSH secundário: Biodegradação Ambiental
Meios de Cultura/química
Pleurotus/metabolismo
Zea mays/química
Zea mays/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Culture Media); 9N2N2Y55MH (Aflatoxin B1)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170829
[Lr] Data última revisão:
170829
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170804
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182574


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[PMID]:28686037
[Au] Autor:Artini M; Cicatiello P; Ricciardelli A; Papa R; Selan L; Dardano P; Tilotta M; Vrenna G; Tutino ML; Giardina P; Parrilli E
[Ad] Endereço:a Department of Public Health and Infectious Diseases , Sapienza University , Rome , Italy.
[Ti] Título:Hydrophobin coating prevents Staphylococcus epidermidis biofilm formation on different surfaces.
[So] Source:Biofouling;33(7):601-611, 2017 Aug.
[Is] ISSN:1029-2454
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Staphylococcus epidermidis is a significant nosocomial pathogen in predisposed hosts because of its capability of forming a biofilm on indwelling medical devices. The initial stage of biofilm formation has a key role in S. epidermidis abiotic surface colonization. Recently, many strategies have been developed to create new anti-biofilm surfaces able to control bacterial adhesion mechanisms. In this work, the self-assembled amphiphilic layers formed by two fungal hydrophobins (Vmh2 and Pac3) have proven to be able to reduce the biofilm formed by different strains of S. epidermidis on polystyrene surfaces. The reduction in the biofilm thickness on the coated surfaces and the preservation of cell vitality have been demonstrated through confocal laser scanning microscope analysis. Moreover, the anti-biofilm efficiency of the self-assembled layers on different medically relevant materials has also been demonstrated using a CDC biofilm reactor.
[Mh] Termos MeSH primário: Aderência Bacteriana/efeitos dos fármacos
Biofilmes/crescimento & desenvolvimento
Proteínas Fúngicas/química
Poliestirenos/química
Staphylococcus epidermidis/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Acremonium/química
Biofilmes/efeitos dos fármacos
Proteínas Fúngicas/isolamento & purificação
Proteínas Fúngicas/farmacologia
Interações Hidrofóbicas e Hidrofílicas
Viabilidade Microbiana/efeitos dos fármacos
Microscopia de Força Atômica
Microscopia Confocal
Pleurotus/química
Staphylococcus epidermidis/efeitos dos fármacos
Staphylococcus epidermidis/fisiologia
Propriedades de Superfície
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungal Proteins); 0 (Polystyrenes)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170708
[St] Status:MEDLINE
[do] DOI:10.1080/08927014.2017.1338690


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[PMID]:28651974
[Au] Autor:Behrens CJ; Linke D; Allister AB; Zelena K; Berger RG
[Ad] Endereço:Gottfried Wilhelm Leibniz Universität Hannover, Institut für Lebensmittelchemie, Callinstraße 5, 30167 Hannover, Germany. Electronic address: Christoph.Behrens@lci.uni-hannover.de.
[Ti] Título:Variants of PpuLcc, a multi-dye decolorizing laccase from Pleurotus pulmonarius expressed in Pichia pastoris.
[So] Source:Protein Expr Purif;137:34-42, 2017 Sep.
[Is] ISSN:1096-0279
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A laccase of the basidiomycete Pleurotus pulmonarius (PpuLcc) possessed strong decolorizing abilities towards artificial and natural dyes. The PpuLcc was purified from the culture supernatant via FPLC, and the corresponding gene cloned and expressed in Pichia pastoris GS115. To examine the impact of the C-terminal tail region and the signal peptide on the recombinant expression of PpuLcc, a non-modified version or different truncations (-2, -5, -13 AA) of the target protein were combined with different secretion signals. Heterologous expression of codon optimized constructs resulted in extracellular activities of the PpuLcc variants of up to 7000 U L (substrate ABTS) which was six times higher than non-codon optimized constructs. In contrast to previous works, altering the C-terminal end of the protein did not influence kinetic parameters or the rate of expression. The His-Tag purified enzymes showed high temperature optima (50-70 °C) and thermo stability. All of the recombinant variants degraded triarylmethane and azo dyes. Rapid bleaching of ß-carotene (E 160a) and the polyene acid norbixin (E 160b) using a laccase was found for the first time. Thus, the enzyme may be useful in decolorizing unwanted polyene pigments, for example from the processing of cheese, bakery, desserts, ice cream or coloured casings.
[Mh] Termos MeSH primário: Corantes/química
Proteínas Fúngicas
Lacase
Pichia/metabolismo
Pleurotus/genética
[Mh] Termos MeSH secundário: Carotenoides/química
Proteínas Fúngicas/biossíntese
Proteínas Fúngicas/química
Proteínas Fúngicas/genética
Proteínas Fúngicas/isolamento & purificação
Lacase/biossíntese
Lacase/química
Lacase/genética
Lacase/isolamento & purificação
Pichia/química
Pichia/genética
Pleurotus/enzimologia
Proteínas Recombinantes/biossíntese
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Proteínas Recombinantes/isolamento & purificação
beta Caroteno/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Coloring Agents); 0 (Fungal Proteins); 0 (Recombinant Proteins); 01YAE03M7J (beta Carotene); 36-88-4 (Carotenoids); E8M59E17AI (norbixin); EC 1.10.3.2 (Laccase)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170807
[Lr] Data última revisão:
170807
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170628
[St] Status:MEDLINE


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[PMID]:28585469
[Au] Autor:Sato T; Irie T; Yoshino F
[Ad] Endereço:a Iwate Biotechnology Research Center , Kitakami-shi , Japan.
[Ti] Título:Heterologous expression of the Pleurotus ostreatus MnP3 gene by the laccase gene promoter in Lentinula edodes.
[So] Source:Biosci Biotechnol Biochem;81(8):1553-1556, 2017 Aug.
[Is] ISSN:1347-6947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Lentinula edodes (shiitake), which have a powerful ligninolytic system, is one of the most important edible mushrooms in Asia. In this study, we introduced the manganese peroxidase (MnP, EC 1.11.1.13) gene from Pleurotus ostreatus driven by L. edodes laccase 1 gene promoter into L. edodes for expression. The resulting transformant expressed the recombinant gene and showed a higher level of MnP activity than that of the wild-type strain.
[Mh] Termos MeSH primário: Proteínas Fúngicas/genética
Regulação Fúngica da Expressão Gênica
Lacase/genética
Peroxidases/genética
Pleurotus/genética
Cogumelos Shiitake/genética
[Mh] Termos MeSH secundário: Ensaios Enzimáticos
Proteínas Fúngicas/metabolismo
Engenharia Genética
Vetores Genéticos/química
Vetores Genéticos/metabolismo
Cinética
Lacase/metabolismo
Peroxidases/metabolismo
Pleurotus/enzimologia
Regiões Promotoras Genéticas
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Cogumelos Shiitake/enzimologia
Transformação Genética
Transgenes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungal Proteins); 0 (Recombinant Proteins); EC 1.10.3.2 (Laccase); EC 1.11.1.- (Peroxidases); EC 1.11.1.13 (manganese peroxidase)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170726
[Lr] Data última revisão:
170726
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170607
[St] Status:MEDLINE
[do] DOI:10.1080/09168451.2017.1332977


  9 / 1240 MEDLINE  
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[PMID]:28551231
[Au] Autor:Poojary MM; Orlien V; Passamonti P; Olsen K
[Ad] Endereço:Department of Food Science, Faculty of Science, University of Copenhagen, Rolighedsvej 26, Frederiksberg C DK-1958, Denmark; School of Science & Technology, Chemistry Section, University of Camerino, via S. Agostino, 1, Camerino 62032, Italy. Electronic address: mahesha@food.ku.dk.
[Ti] Título:Enzyme-assisted extraction enhancing the umami taste amino acids recovery from several cultivated mushrooms.
[So] Source:Food Chem;234:236-244, 2017 Nov 01.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In this study, enzyme-assisted extraction was performed to extract umami taste and total free amino acids (FAAs) from the six different mushrooms including shiitake (Lentinus edodes), oyster (Pleurotus ostreatus), tea tree (Agrocybe aegerita) and, white, brown and portobello champignons (Agaricus bisporus). ß-Glucanase and Flavourzyme® were used as the enzymes for cell wall and proteins hydrolysis, respectively. It was found that ß-glucanase treatment alone did not enhance the extraction efficiency, however in combination, ß-glucanase and Flavourzyme® enhanced the extraction efficiency significantly up to 20-fold compared to conventional HCl mediated extraction, depending on the mushroom species. The optimal conditions for the enzyme treatment were: water as extraction solvent (initial pH = 7), enzyme concentration of 5% v/w each of ß-glucanase and Flavourzyme®, temperature 50°C and an incubation time of 1h. White and brown champignons were found to be the richest source of umami taste FAAs (26.75±1.07 and 25.6±0.9mg/g DM, respectively).
[Mh] Termos MeSH primário: Agaricus/química
Aminoácidos/isolamento & purificação
Enzimas/química
Pleurotus/química
Cogumelos Shiitake/química
Paladar
[Mh] Termos MeSH secundário: Solventes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Enzymes); 0 (Solvents)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170808
[Lr] Data última revisão:
170808
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170529
[St] Status:MEDLINE


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[PMID]:28534588
[Au] Autor:Cardoso RVC; Fernandes Â; Oliveira MBPP; Calhelha RC; Barros L; Martins A; Ferreira ICFR
[Ad] Endereço:Centro de Investigação de Montanha (CIMO), ESA, Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253 Bragança, Portugal. lillian@ipb.pt iferreira@ipb.pt.
[Ti] Título:Development of nutraceutical formulations based on the mycelium of Pleurotus ostreatus and Agaricus bisporus.
[So] Source:Food Funct;8(6):2155-2164, 2017 Jun 21.
[Is] ISSN:2042-650X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The present work is aimed at developing nutraceutical formulations based on the mycelium of Agaricus bisporus and Pleurotus ostreatus, highlighting the potential of in vitro culture as a tool to improve the production of bioactive compounds, namely phenolic acids and ergosterol. The mycelia of both species were cultured in different solid and liquid media in order to compare the growth rate and yielded biomass. Fruiting bodies, mycelia and culture media were compared regarding the antioxidant activity, anti-inflammatory effects in RAW264.7 cells and cytotoxicity in human tumor cell lines and non-tumor porcine liver cells. P. ostreatus mycelia showed higher contents of ergosterol and phenolic compounds, and stronger antioxidant activity than the corresponding fruiting body. P. ostreatus and A. bisporus did not show anti-inflammatory activity, and P. ostreatus was the only one showing cytotoxicity in tumor cell lines. The results show that these mushrooms provide compounds with antioxidant and cytotoxic capacities, with variations among species.
[Mh] Termos MeSH primário: Agaricus/química
Suplementos Nutricionais/análise
Micélio/química
Extratos Vegetais/análise
Pleurotus/química
[Mh] Termos MeSH secundário: Agaricus/crescimento & desenvolvimento
Agaricus/metabolismo
Antioxidantes/análise
Antioxidantes/metabolismo
Antioxidantes/farmacologia
Linhagem Celular Tumoral
Sobrevivência Celular/efeitos dos fármacos
Meios de Cultura/química
Meios de Cultura/metabolismo
Carpóforos/química
Carpóforos/crescimento & desenvolvimento
Carpóforos/metabolismo
Seres Humanos
Micélio/crescimento & desenvolvimento
Micélio/metabolismo
Fenóis/análise
Fenóis/metabolismo
Fenóis/farmacologia
Extratos Vegetais/metabolismo
Extratos Vegetais/farmacologia
Pleurotus/crescimento & desenvolvimento
Pleurotus/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Culture Media); 0 (Phenols); 0 (Plant Extracts)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170901
[Lr] Data última revisão:
170901
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170524
[St] Status:MEDLINE
[do] DOI:10.1039/c7fo00515f



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