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[PMID]: 28653896 [Au] Autor: Gill BS; Navgeet; Kumar S [Ad] Endereço: 1 Centre for Biosciences, Central University of Punjab, Bathinda, India. [Ti] Título: Ganoderma lucidum targeting lung cancer signaling: A review. [So] Source: Tumour Biol;39(6):1010428317707437, 2017 Jun. [Is] ISSN: 1423-0380 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Lung cancer causes huge mortality to population, and pharmaceutical companies require new drugs as an alternative either synthetic or natural targeting lung cancer. The conventional therapies cause side effects, and therefore, natural products are used as a therapeutic candidate in lung cancer. Chemical diversity among natural products highlights the impact of evolution and survival of fittest. One such neglected natural product is Ganoderma lucidum used for promoting health and longevity for a longer time. The major bioconstituents of G. lucidum are mainly terpenes, polysaccharides, and proteins, which were explored for various activities ranging from apoptosis to autophagy. The bioconstituents of G. lucidum activate plasma membrane receptors and initiate various downstream signaling leading to nuclear factor-κB, phosphoinositide 3-kinase, Akt, and mammalian target of rapamycin in cancer. The bioconstituents regulate the expression of various genes involved in cell cycle, immune response, apoptosis, and autophagy in lung cancer. This review highlights the inextricable role of G. lucidum and its bioconstituents in lung cancer signaling for the first time. [Mh] Termos MeSH primário: Medicamentos de Ervas Chinesas/uso terapêutico Neoplasias Pulmonares/tratamento farmacológico Transdução de Sinais/genética [Mh] Termos MeSH secundário: Apoptose/efeitos dos fármacos Autofagia/efeitos dos fármacos Ciclo Celular/efeitos dos fármacos Medicamentos de Ervas Chinesas/química Seres Humanos Neoplasias Pulmonares/genética Neoplasias Pulmonares/patologia NF-kappa B/genética Proteína Oncogênica v-akt/genética Fosfatidilinositol 3-Quinases/genética Reishi/química [Pt] Tipo de publicação: JOURNAL ARTICLE; REVIEW [Nm] Nome de substância: 0 (Drugs, Chinese Herbal); 0 (NF-kappa B); EC 2.7.1.- (Phosphatidylinositol 3-Kinases); EC 2.7.11.1 (Oncogene Protein v-akt) [Em] Mês de entrada: 1707 [Cu] Atualização por classe: 170713 [Lr] Data última revisão: 170713 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170628 [St] Status: MEDLINE [do] DOI: 10.1177/1010428317707437

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[PMID]: 28527898 [Au] Autor: Chen B; Tian J; Zhang J; Wang K; Liu L; Yang B; Bao L; Liu H [Ad] Endereço: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, No.1 Beichenxi Road, Chaoyang District, Beijing 100101, PR China; Savaid Medicine School, University of Chinese Academy of Sciences, Beijing 100049, PR China. [Ti] Título: Triterpenes and meroterpenes from Ganoderma lucidum with inhibitory activity against HMGs reductase, aldose reductase and α-glucosidase. [So] Source: Fitoterapia;120:6-16, 2017 Jul. [Is] ISSN: 1873-6971 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: Seven new compounds including four lanostane triterpenoids, lucidenic acids Q-S (1-3) and methyl ganoderate P (4), and three triterpene-farnesyl hydroquinone conjugates, ganolucinins A-C (5-7), one new natural product ganomycin J (8), and 73 known compounds (9-81) were isolated from fruiting bodies of Ganoderma lucidum. The structures of the compounds 1-8 were determined by spectroscopic methods. Bioactivities of compounds isolated were assayed against HMG-CoA reductase, aldose reductase, α-glucosidase, and PTP1B. Ganolucidic acid η (39), ganoderenic acid K (44), ganomycin J (8), and ganomycin B (61) showed strong inhibitory activity against HMG-CoA reductase with IC of 29.8, 16.5, 30.3 and 14.3µM, respectively. Lucidumol A (67) had relatively good effect against aldose reductase with IC of 19.1µM. Farnesyl hydroquinones ganomycin J (8), ganomycin B (61), ganomycin I (62), and triterpene-farnesyl hydroquinone conjugates ganoleuconin M (76) and ganoleuconin O (79) possessed good inhibitory activity against α-glucosidase with IC in the range of 7.8 to 21.5µM. This work provides chemical and biological evidence for the usage of extracts of G. lucidum as herbal medicine and food supplements for the control of hyperglycemic and hyperlipidemic symptoms. [Mh] Termos MeSH primário: Aldeído Redutase/antagonistas & inibidores Inibidores de Glicosídeo Hidrolases/química Inibidores de Hidroximetilglutaril-CoA Redutases/química Reishi/química Triterpenos/química [Mh] Termos MeSH secundário: Animais Bovinos Linhagem Celular Tumoral Inibidores de Glicosídeo Hidrolases/isolamento & purificação Seres Humanos Hidroximetilglutaril-CoA Redutases Inibidores de Hidroximetilglutaril-CoA Redutases/isolamento & purificação Estrutura Molecular Ratos alfa-Glucosidases [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Glycoside Hydrolase Inhibitors); 0 (Hydroxymethylglutaryl-CoA Reductase Inhibitors); 0 (Triterpenes); EC 1.1.1.- (Hydroxymethylglutaryl CoA Reductases); EC 1.1.1.21 (Aldehyde Reductase); EC 3.2.1.20 (alpha-Glucosidases) [Em] Mês de entrada: 1707 [Cu] Atualização por classe: 170731 [Lr] Data última revisão: 170731 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170522 [St] Status: MEDLINE

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[PMID]: 28427938 [Au] Autor: Zhang Y [Ad] Endereço: The State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China. Electronic address: zhangyu14@mails.tsinghua.edu.cn. [Ti] Título: Ganoderma lucidum (Reishi) suppresses proliferation and migration of breast cancer cells via inhibiting Wnt/ß-catenin signaling. [So] Source: Biochem Biophys Res Commun;488(4):679-684, 2017 Jul 08. [Is] ISSN: 1090-2104 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: The medical mushroom Ganoderma lucidum (Reishi), a traditional Chinese medicine, has exhibited a promising anti-cancer effect. However, the molecular mechanism of its action on cancer cells remains unclear. Aberrant activation of Wnt/ß-catenin signaling pathway is the cause of many types of cancer, including breast cancer. Here we investigated the effect of Reishi on Wnt/ß-catenin signaling pathway and elucidated the molecular mechanism of its function in inhibiting breast cancer cells. We found that Reishi blocked Wnt/ß-catenin signaling through inhibiting the phosphorylation of Wnt co-receptor LRP6. In human (MDA-MB-231) and mouse (4T1) breast cancer cell lines, Reishi significantly decreased the phosphorylation of LRP6 and suppressed Wnt3a-activated Wnt target gene Axin2 expression. Administration of Reishi inhibited Wnt-induced hyper-proliferation of breast cancer cells and MDA-MB-231 cell migration. Our results provide evidence that Reishi suppresses breast cancer cell growth and migration through inhibiting Wnt/ß-catenin signaling, indicating that Reishi may be a potential natural inhibitor for breast cancer. [Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia Neoplasias da Mama/tratamento farmacológico Neoplasias da Mama/patologia Movimento Celular/efeitos dos fármacos Medicamentos de Ervas Chinesas/farmacologia Reishi/química Via de Sinalização Wnt/efeitos dos fármacos [Mh] Termos MeSH secundário: Animais Antineoplásicos Fitogênicos/química Antineoplásicos Fitogênicos/isolamento & purificação Neoplasias da Mama/metabolismo Proliferação Celular/efeitos dos fármacos Células Cultivadas Relação Dose-Resposta a Droga Ensaios de Seleção de Medicamentos Antitumorais Medicamentos de Ervas Chinesas/química Medicamentos de Ervas Chinesas/isolamento & purificação Feminino Células HEK293 Seres Humanos Medicina Tradicional Chinesa Camundongos Relação Estrutura-Atividade Proteínas Wnt/metabolismo beta Catenina/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antineoplastic Agents, Phytogenic); 0 (Drugs, Chinese Herbal); 0 (Wnt Proteins); 0 (beta Catenin) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171002 [Lr] Data última revisão: 171002 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170422 [St] Status: MEDLINE

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[PMID]: 28383512 [Au] Autor: Wu L; Liang W; Chen W; Li S; Cui Y; Qi Q; Zhang L [Ad] Endereço: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China. fanglingwu@163.com. [Ti] Título: Screening and Analysis of the Marker Components in Ganoderma lucidum by HPLC and HPLC-MS with the Aid of Chemometrics. [So] Source: Molecules;22(4), 2017 Apr 06. [Is] ISSN: 1420-3049 [Cp] País de publicação: Switzerland [La] Idioma: eng [Ab] Resumo: triterpenes (GTs) are the major secondary metabolites of , which is a popularly used traditional Chinese medicine for complementary cancer therapy. The present study was to establish a fingerprint evaluation system based on Similarity Analysis (SA), Cluster Analysis (CA) and Principal Component Analysis (PCA) for the identification and quality control of . Fifteen samples from the Chinese provinces of Hainan, Neimeng, Shangdong, Jilin, Anhui, Henan, Yunnan, Guangxi and Fujian were analyzed by HPLC-PAD and HPLC-MS . Forty-seven compounds were detected by HPLC, of which forty-two compounds were tentatively identified by comparing their retention times and mass spectrometry data with that of reference compounds and reviewing the literature. Ganoderic acid B, 3,7,15-trihydroxy-11,23-dioxolanost-8,16-dien-26-oic acid, lucidenic acid A, ganoderic acid G, and 3,7-oxo-12-acetylganoderic acid DM were deemed to be the marker compounds to distinguish the samples with different quality according to both CA and PCA. This study provides helpful chemical information for further research on the anti-tumor activity and mechanism of action of . The results proved that fingerprints combined with chemometrics are a simple, rapid and effective method for the quality control of . [Mh] Termos MeSH primário: Produtos Biológicos/química Reishi/química [Mh] Termos MeSH secundário: Cromatografia Líquida de Alta Pressão Análise por Conglomerados Espectrometria de Massas Estrutura Molecular Análise de Componente Principal Reprodutibilidade dos Testes [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Biological Products) [Em] Mês de entrada: 1705 [Cu] Atualização por classe: 170531 [Lr] Data última revisão: 170531 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170407 [St] Status: MEDLINE

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[PMID]: 28358412 [Au] Autor: Na K; Li K; Sang T; Wu K; Wang Y; Wang X [Ad] Endereço: College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, P.R. China. [Ti] Título: Anticarcinogenic effects of water extract of sporoderm-broken spores of Ganoderma lucidum on colorectal cancer in vitro and in vivo. [So] Source: Int J Oncol;50(5):1541-1554, 2017 May. [Is] ISSN: 1791-2423 [Cp] País de publicação: Greece [La] Idioma: eng [Ab] Resumo: Ganoderma lucidum (G. lucidum) polysaccharides (GLPs) have been used as traditional Chinese medicine for cancer prevention for many years. However, the mechanism by which GLP exerts its chemopreventive activities remains elusive. In addition, it is unclear whether sporoderm-broken spores of G. lucidum water extract (BSGLWE), which contains mainly GLPs, has anticancer effects on colorectal cancer. The present study investigated the anticancer effects and potential mechanisms of BSGLWE on colorectal cancer in vivo and in vitro. Our results showed that BSGLWE significantly inhibited colorectal cancer HCT116 cell viability in a time- and dose-dependent manner. Flow cytometry analysis indicated that BSGLWE disrupted cell cycle progression at G2/M phase via downregulation of cyclin B1 and cyclin A2, and upregulation of P21 at mRNA levels. Moreover, BSGLWE induced apoptosis by decreasing Bcl-2 and survivin at mRNA levels, and reduced Bcl-2, PARP, pro-caspase-3 and pro-caspase-9 at protein levels. Furthermore, BSGLWE suppressed tumor growth in vivo by regulating the expression of genes and proteins associated with cell cycle and apoptosis, which was further confirmed by a reduction of Ki67, PCNA, and Bcl-2 expression as determined by immunohistochemistry staining. NSAID activated gene-1 (NAG-1), a pro-apoptotic gene, was significantly upregulated in vivo and in vitro upon BSGLWE treatment at both mRNA and protein levels. In addition, the relative amounts of secreted NAG-1 in cell culture medium or serum of nude mice were all upregulated upon BSGLWE treatments, suggesting a role of NAG-1 in BSGLWE-induced anticolorectal cancer activity. This is the first study to show that BSGLWE inhibits colorectal cancer carcinogenesis through regulating genes responsible for cell proliferation, cell cycle and apoptosis cascades. These findings indicate that BSGLWE possesses chemopreventive potential in colorectal cancer which may serve as a promising anticancer agent for clinical applications. [Mh] Termos MeSH primário: Anticarcinógenos/administração & dosagem Neoplasias Colorretais/tratamento farmacológico Extratos Vegetais/administração & dosagem Reishi/química [Mh] Termos MeSH secundário: Animais Anticarcinógenos/química Divisão Celular/efeitos dos fármacos Proliferação Celular/efeitos dos fármacos Neoplasias Colorretais/patologia Células HCT116 Seres Humanos Medicina Tradicional Chinesa Camundongos Extratos Vegetais/química Água/química Ensaios Antitumorais Modelo de Xenoenxerto [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Anticarcinogenic Agents); 0 (Plant Extracts); 059QF0KO0R (Water) [Em] Mês de entrada: 1707 [Cu] Atualização por classe: 170726 [Lr] Data última revisão: 170726 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170331 [St] Status: MEDLINE [do] DOI: 10.3892/ijo.2017.3939

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[PMID]: 28322148 [Au] Autor: Cao PF; Wu CG; Dang ZH; Shi L; Jiang AL; Ren A; Zhao MW [Ad] Endereço: College of Life Sciences, Nanjing Agricultural University, Nanjing, People's Republic of China; Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Nanjing, Jiangsu, People's Republic of China. [Ti] Título: Effects of Exogenous Salicylic Acid on Ganoderic Acid Biosynthesis and the Expression of Key Genes in the Ganoderic Acid Biosynthesis Pathway in the Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes). [So] Source: Int J Med Mushrooms;19(1):65-73, 2017. [Is] ISSN: 1940-4344 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: We demonstrate herein that salicylic acid (SA) can enhance ganoderic acid (GA) accumulation in the lingzhi or reishi medicinal mushroom Ganoderma lucidum. Following treatment with different concentrations of SA, the GA content was increased 22.72% to 43.04% compared with the control group. When the fungi were treated with 200 µmol/L SA at different times, the GA content was improved 10.21% to 35.24% compared with the control group. By choosing the optimum point based on response surface methodology, the GA content could be increased up to 229.03 µg/100 mg, which was improved 66.38% compared with the control group. When the fungi were treated with 200 µmol/L SA, the transcription levels of key genes in the GA biosynthesis pathway-squalene (SQ) synthase (sqs), lanosterol (Lano; osc), and hydroxy-3-methylglutaryl-coenzyme A reductase (hmgr)-were improved 119.6-, 3.2-, and 4.2-fold, respectively. In addition, following treatment with 100 µmol/L SA, the levels of Lano and SQ, which are intermediate metabolites of GA biosynthesis, were increased 2.8- and 1.4-fold, respectively. These results indicate that SA can regulate the expression of genes related to GA biosynthesis and increases the metabolic levels of Lano and SQ, thereby resulting in the accumulation of GA. [Mh] Termos MeSH primário: Vias Biossintéticas/efeitos dos fármacos Perfilação da Expressão Gênica Regulação da Expressão Gênica/efeitos dos fármacos Reishi/efeitos dos fármacos Reishi/metabolismo Ácido Salicílico/metabolismo Triterpenos/metabolismo [Mh] Termos MeSH secundário: Reishi/genética [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Triterpenes); 0 (ganoderic acid); O414PZ4LPZ (Salicylic Acid) [Em] Mês de entrada: 1704 [Cu] Atualização por classe: 170407 [Lr] Data última revisão: 170407 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170322 [St] Status: MEDLINE [do] DOI: 10.1615/IntJMedMushrooms.v19.i1.70

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[PMID]: 28264501 [Au] Autor: Barbieri A; Quagliariello V; Del Vecchio V; Falco M; Luciano A; Amruthraj NJ; Nasti G; Ottaiano A; Berretta M; Iaffaioli RV; Arra C [Ad] Endereço: Animal Facility Unit, Department of Research, National Cancer Institute "G. Pascale", Via M. Semmola, 80131 Naples, Italy. a.barbieri@istitutotumori.na.it. [Ti] Título: Anticancer and Anti-Inflammatory Properties of Ganoderma lucidum Extract Effects on Melanoma and Triple-Negative Breast Cancer Treatment. [So] Source: Nutrients;9(3), 2017 Feb 28. [Is] ISSN: 2072-6643 [Cp] País de publicação: Switzerland [La] Idioma: eng [Ab] Resumo: Among the most important traditional medicinal fungi, has been used as a therapeutic agent for the treatment of numerous diseases, including cancer, in Oriental countries. The aim of this study is to investigate the anti-inflammatory, anticancer and anti-metastatic activities of extracts in melanoma and triple-negative breast cancer cells. extracts were prepared by using common organic solvents; MDA-MB 231 and B16-F10 cell lines were adopted as cellular models for triple-negative breast cancer and melanoma and characterized for cell viability, wound-healing assay and measurement of cytokines secreted by cancer cells under pro-inflammatory conditions (incubation with lipopolysaccharide, LPS) and pretreatment with extract at different concentrations. Our study demonstrates, for the first time, how extracts can significantly inhibit the release of IL-8, IL-6, MMP-2 and MMP-9 in cancer cells under pro-inflammatory condition. Interestingly, extracts significantly also decrease the viability of both cancer cells in a time- and concentration-dependent manner, with abilities to reduce cell migration over time, which is correlated with a lower release of matrix metalloproteases. Taken together, these results indicate the possible use of extract for the therapeutic management of melanoma and human triple-negative breast cancer. [Mh] Termos MeSH primário: Anti-Inflamatórios/farmacologia Antineoplásicos/farmacologia Melanoma/patologia Reishi/química Neoplasias de Mama Triplo Negativas/patologia [Mh] Termos MeSH secundário: Animais Linhagem Celular Tumoral Proliferação Celular/efeitos dos fármacos Seres Humanos Interleucina-6/metabolismo Interleucina-8/metabolismo Metaloproteinase 2 da Matriz/metabolismo Metaloproteinase 9 da Matriz/metabolismo Melanoma/tratamento farmacológico Camundongos Camundongos Endogâmicos C57BL Neoplasias de Mama Triplo Negativas/tratamento farmacológico [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Anti-Inflammatory Agents); 0 (Antineoplastic Agents); 0 (Interleukin-6); 0 (Interleukin-8); EC 3.4.24.24 (Matrix Metalloproteinase 2); EC 3.4.24.35 (Matrix Metalloproteinase 9) [Em] Mês de entrada: 1708 [Cu] Atualização por classe: 170817 [Lr] Data última revisão: 170817 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170308 [St] Status: MEDLINE

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[PMID]: 28231778 [Au] Autor: Xu H; Guo MY; Gao YH; Bai XH; Zhou XW [Ad] Endereço: Key Laboratory of Urban Agriculture (South) Ministry of Agriculture, and Engineering Research Center of Cell & Therapeutic Antibody, Ministry of Education, and School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, People's Republic of China. [Ti] Título: Expression and characteristics of manganese peroxidase from Ganoderma lucidum in Pichia pastoris and its application in the degradation of four dyes and phenol. [So] Source: BMC Biotechnol;17(1):19, 2017 Feb 23. [Is] ISSN: 1472-6750 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: BACKGROUND: Manganese peroxidase (MnP) of white rot basidiomycetes, an extracellular heme enzyme, is part of a peroxidase superfamily that is capable of degrading the different phenolic compounds. Ganoderma, a white rot basidiomycete widely distributed worldwide, could secrete lignin-modifying enzymes (LME), including laccase (Lac), lignin peroxidases (LiP) and MnP. RESULTS: After the selection of a G. lucidum strain from five Ganoderma strains, the 1092 bp full-length cDNA of the MnP gene, designated as G. lucidum MnP (GluMnP1), was cloned from the selected strain. We subsequently constructed an eukaryotic expression vector, pAO815:: GlMnP, and transferred it into Pichia pastoris SMD116. Recombinant GluMnP1 (rGluMnP1) was with a yield of 126 mg/L and a molecular weight of approximately 37.72 kDa and a specific enzyme activity of 524.61 U/L. The rGluMnP1 could be capable of the decolorization of four types of dyes and the degradation of phenol. Phenol and its principal degradation products including hydroquinone, pyrocatechol, resorcinol, benzoquinone, were detected successfully in the experiments. CONCLUSIONS: The rGluMnP1 could be effectively expressed in Pichia pastoris and with a higher oxidation activity. We infer that, in the initial stages of the reaction, the catechol-mediated cycle should be the principal route of enzymatic degradation of phenol and its oxidation products. This study highlights the potential industrial applications associated with the production of MnP by genetic engineering methods, and the application of industrial wastewater treatment. [Mh] Termos MeSH primário: Corantes/química Peroxidases/química Fenol/química Pichia/enzimologia Reishi/enzimologia Poluentes Químicos da Água/química [Mh] Termos MeSH secundário: Biodegradação Ambiental Clonagem Molecular/métodos Corantes/isolamento & purificação Ativação Enzimática Peroxidases/genética Peroxidases/metabolismo Fenol/isolamento & purificação Pichia/genética Engenharia de Proteínas/métodos Proteínas Recombinantes/genética Proteínas Recombinantes/metabolismo Reishi/classificação Reishi/genética Especificidade da Espécie Poluentes Químicos da Água/isolamento & purificação Purificação da Água/métodos [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Coloring Agents); 0 (Recombinant Proteins); 0 (Water Pollutants, Chemical); 339NCG44TV (Phenol); EC 1.11.1.- (Peroxidases); EC 1.11.1.13 (manganese peroxidase) [Em] Mês de entrada: 1707 [Cu] Atualização por classe: 170704 [Lr] Data última revisão: 170704 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170225 [St] Status: MEDLINE [do] DOI: 10.1186/s12896-017-0338-5

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[PMID]: 28199921 [Au] Autor: Postemsky PD; Bidegain MA; González-Matute R; Figlas ND; Cubitto MA [Ad] Endereço: Centro de Recursos Renovables de la Zona Semiárida (CERZOS), Universidad Nacional del Sur (UNS), CONICET, Laboratorio de Biotecnología de Hongos Comestibles y Medicinales, Camino de la Carrindanga Km7, Bahía Blanca (8000), Buenos Aires, Argentina. Electronic address: pablop@criba.edu.ar. [Ti] Título: Pilot-scale bioconversion of rice and sunflower agro-residues into medicinal mushrooms and laccase enzymes through solid-state fermentation with Ganoderma lucidum. [So] Source: Bioresour Technol;231:85-93, 2017 May. [Is] ISSN: 1873-2976 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Solid-state fermentation was evaluated at the pilot-scale for the bioconversion and valorization of rice husks and straw (RSH), or sunflower seed hulls (SSH), into medicinal mushrooms and crude extracts, with laccase activity. The average mushroom yield was 56kg dry weight per ton of agro-residues. Laccase activity in crude aqueous extracts showed its maximum value of 10,927Ukg in RSH (day 10, Exudate phase) and 16,442Ukg in SSH (day 5, Full colonization phase), the activity at the Residual substrate phase being 511Ukg in RSH and 803Ukg in SSH, respectively. Crude extracts obtained with various protocols revealed differences in the extraction yields. Lyophilization followed by storage at 4°C allowed the preservation of laccase activity for more than one month. It is proposed that standard mushroom farms could increase their profits by obtaining laccase as a byproduct during the gaps in mycelium running. [Mh] Termos MeSH primário: Agaricales/metabolismo Fermentação Helianthus/metabolismo Lacase/metabolismo Oryza/metabolismo Reishi/metabolismo [Mh] Termos MeSH secundário: Biomassa Metabolismo dos Carboidratos Micélio/metabolismo Projetos Piloto Especificidade por Substrato [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: EC 1.10.3.2 (Laccase) [Em] Mês de entrada: 1704 [Cu] Atualização por classe: 170424 [Lr] Data última revisão: 170424 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170216 [St] Status: MEDLINE

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[PMID]: 28198137 [Au] Autor: Liu YN; Zhang TJ; Lu XX; Ma BL; Ren A; Shi L; Jiang AL; Yu HS; Zhao MW [Ad] Endereço: Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Microbiology Department, College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, P.R. China. [Ti] Título: Membrane fluidity is involved in the regulation of heat stress induced secondary metabolism in Ganoderma lucidum. [So] Source: Environ Microbiol;19(4):1653-1668, 2017 Apr. [Is] ISSN: 1462-2920 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Ganoderma lucidum has become a potential model system for evaluating how environmental factors regulate the secondary metabolism of basidiomycetes. Heat stress (HS) is one of the most important environmental factors. It was previously reported that HS could induce the biosynthesis of ganoderic acids (GA). In this study, we found that HS increased GA biosynthesis and also significantly increased cell membrane fluidity. Furthermore, our results showed that addition of the membrane rigidifier dimethylsulfoxide (DMSO) could revert the increased GA biosynthesis elicited by HS. These results indicate that an increase in membrane fluidity is associated with HS-induced GA biosynthesis. Further evidence showed that the GA content was decreased in D9des-silenced strains and could be reverted to WT levels by addition of the membrane fluidizer benzyl alcohol (BA). In contrast, GA content was increased in D9des-overexpression strains and could be reverted to WT levels by the addition of DMSO. Furthermore, both membrane fluidity and GA biosynthesis induced by HS could be reverted by DMSO in WT and D9des-silenced strains. To the best of our knowledge, this is the first report demonstrating that membrane fluidity is involved in the regulation of heat stress induced secondary metabolism in filamentous fungi. [Mh] Termos MeSH primário: Resposta ao Choque Térmico Fluidez de Membrana Reishi/metabolismo [Mh] Termos MeSH secundário: Temperatura Alta Metabolismo Secundário Triterpenos [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Triterpenes); 0 (ganoderic acid) [Em] Mês de entrada: 1708 [Cu] Atualização por classe: 170821 [Lr] Data última revisão: 170821 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170216 [St] Status: MEDLINE [do] DOI: 10.1111/1462-2920.13693

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