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[PMID]:28510717
[Au] Autor:Bussaman P; Sa-Uth C; Chandrapatya A; Atlihan R; Gökçe A; Saska P; Chi H
[Ad] Endereço:Biological Control Research Unit, Department of Biotechnology, Faculty of Technology, Mahasarakham University, Mahasarakham 44150, Thailand.
[Ti] Título:Fast Population Growth in Physogastry Reproduction of Luciaphorus perniciosus (Acari: Pygmephoridae) at Different Temperatures.
[So] Source:J Econ Entomol;110(4):1397-1403, 2017 Aug 01.
[Is] ISSN:1938-291X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Luciaphorus perniciosus Rack is one of the most serious pests of several cultivated mushroom species including Ganoderma lucidum (Fr.), Flammulina velutipes Karst., Auricularia polytricha (Mont.) Saac., Lentinus polychrous Lev., and Lentinus squarrosulus (Mont.) Singer in Thailand. Adult female Lu. perniciosus produce offspring inside their physogastric hysterosomas, with all embryos developing through to the adult stage while remaining in the abdomen. Once the abdomen ruptures, the female parent dies and the offspring consisting of mostly fertilized female adults along with a few male adults continue to emerge from the cadaver of the mother for a period of several days. This peculiar type of reproduction after the death of the mother is a special case for life table analysis and has not been discussed previously in demographic analyses. In this study, the life table data of this mite fed on Le. squarrosulus were collected at 25, 30, and 35 °C and analyzed by using the age-stage, two-sex life table. The standard errors of population parameters were estimated by using the bootstrap technique (200,000 bootstraps). At 25, 30, and 35 °C, females started reproduction at ages 9, 5, and 3 d, respectively; the net reproductive rates (R0) were 192.27, 253.81, and 234.11 offspring. Due to their rapid development and high fecundity, the r values were as high as 0.4189, 0.8653, and 1.0892 d-1 at 25, 30, and 35 °C, respectively. Computer projection indicated that the mushroom mites Lu. perniciosus is capable of a threefold daily increase at 35 °C.
[Mh] Termos MeSH primário: Cadeia Alimentar
Ácaros/fisiologia
Polyporaceae/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Animais
Feminino
Herbivoria
Tábuas de Vida
Masculino
Crescimento Demográfico
Reprodução
Temperatura Ambiente
Tailândia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170517
[St] Status:MEDLINE
[do] DOI:10.1093/jee/tox102


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[PMID]:28221210
[Au] Autor:Lim DS; Tan PL; Jureen R; Tan KB
[Ad] Endereço:*Yong Loo Lin School of Medicine, National University of Singapore, Singapore; †Division of Paediatric Haematology and Oncology, Khoo Teck Puat-National University Children's Institute, National University Health System, Singapore; ‡Division of Microbiology, Department of Laboratory Medicine, National University Health System, Singapore; and §Department of Pathology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore, Singapore.
[Ti] Título:Cutaneous Emboli of Invasive Basidiomycosis in a Child With Aplastic Anemia.
[So] Source:Am J Dermatopathol;39(3):204-207, 2017 Mar.
[Is] ISSN:1533-0311
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Invasive fungal diseases are a significant cause of mortality among the immunocompromised. This report documents an unusual case of disseminated fungal infection in a child with severe aplastic anemia. The offending fungus, a Basidiomycete, is rarely known to cause human infections. The patient presented acutely with multiple purpuric skin lesions in various parts of the body. The skin biopsy revealed septated fungal hyphae embolized within small dermal blood vessels. Molecular sequencing indicated Earliella scabrosa as the likely organism. The clinical course of the infection was inexorable despite systemic antifungal treatment, resulting in mortality. The literature of human infections due to Basidiomycetes, the usefulness of histopathology in the early diagnosis of the infection, and possible treatment options are discussed.
[Mh] Termos MeSH primário: Anemia Aplástica/complicações
Dermatomicoses/imunologia
Hospedeiro Imunocomprometido
Infecções Oportunistas/imunologia
[Mh] Termos MeSH secundário: Dermatomicoses/microbiologia
Embolia/microbiologia
Evolução Fatal
Seres Humanos
Lactente
Masculino
Polyporaceae
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170314
[Lr] Data última revisão:
170314
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170222
[St] Status:MEDLINE
[do] DOI:10.1097/DAD.0000000000000699


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[PMID]:28123132
[Au] Autor:Hamada S; Kubota K; Sagisaka M
[Ad] Endereço:Faculty of Agriculture and Life Science, Hirosaki University.
[Ti] Título:Purification and characterization of a novel extracellular neutral metalloprotease from Cerrena albocinnamomea.
[So] Source:J Gen Appl Microbiol;63(1):51-57, 2017 Mar 17.
[Is] ISSN:1349-8037
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:We selected a fungus secreting a neutral protease from soil and identified it as the basidiomycete fungus Cerrena albocinnamomea according to its ITS-5.8S rDNA and 28S rDNA-D1/D2 sequences. A major extracellular protease isolated from C. albocinnamomea was purified approximately 44-fold through two purification steps. SDS-PAGE analyses of the purified protease revealed a single band, and its molecular mass of 39,756 Da was determined using MALDI-TOF-MS. The enzyme was optimally active at approximately pH 7.0 and 45°C. The K and V values for the hydrolysis of azocasein were 2.46 mg/mL and 989 units/min/mg protein, respectively. The enzyme was stable at pH 3.6-8.6 for 16 h and at temperatures ≤35°C for 1 h. Enzymatic activity was completely inhibited by Cu and Zn and markedly by EDTA and phosphoramidon. The N-terminal amino acid sequence ASYRVLPIT is highly similar to those of the members of the metalloprotease family M36, such as keratinase and elastinase. However, the protease did not detectably hydrolyze keratin or elastin. In contrast, the protease hydrolyzed fibrinogen, although there were no significant sequence similarities to the N-terminal amino acid sequences of other fibrinolytic enzymes. These results suggest that the purified protease represents a new neutral metalloprotease with fibrinogenolytic activity.
[Mh] Termos MeSH primário: Metaloproteases/isolamento & purificação
Metaloproteases/metabolismo
Polyporaceae/enzimologia
[Mh] Termos MeSH secundário: Caseínas/metabolismo
Análise por Conglomerados
DNA Fúngico/química
DNA Fúngico/genética
DNA Ribossômico/química
DNA Ribossômico/genética
DNA Espaçador Ribossômico/química
DNA Espaçador Ribossômico/genética
Eletroforese em Gel de Poliacrilamida
Estabilidade Enzimática
Concentração de Íons de Hidrogênio
Cinética
Metaloproteases/química
Peso Molecular
Filogenia
Polyporaceae/classificação
Polyporaceae/genética
Polyporaceae/isolamento & purificação
Inibidores de Proteases/análise
RNA Ribossômico 28S/genética
RNA Ribossômico 5,8S/genética
Análise de Sequência de DNA
Microbiologia do Solo
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
Especificidade por Substrato
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Caseins); 0 (DNA, Fungal); 0 (DNA, Ribosomal); 0 (DNA, Ribosomal Spacer); 0 (Protease Inhibitors); 0 (RNA, Ribosomal, 28S); 0 (RNA, Ribosomal, 5.8S); 0 (azocasein); EC 3.4.- (Metalloproteases)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170417
[Lr] Data última revisão:
170417
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170127
[St] Status:MEDLINE
[do] DOI:10.2323/jgam.2016.07.006


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[PMID]:28028889
[Au] Autor:Rytioja J; Hildén K; Di Falco M; Zhou M; Aguilar-Pontes MV; Sietiö OM; Tsang A; de Vries RP; Mäkelä MR
[Ad] Endereço:Division of Microbiology and Biotechnology, Department of Food and Environmental Sciences, University of Helsinki, Helsinki, Finland.
[Ti] Título:The molecular response of the white-rot fungus Dichomitus squalens to wood and non-woody biomass as examined by transcriptome and exoproteome analyses.
[So] Source:Environ Microbiol;19(3):1237-1250, 2017 Mar.
[Is] ISSN:1462-2920
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The ability to obtain carbon and energy is a major requirement to exist in any environment. For several ascomycete fungi, (post-)genomic analyses have shown that species that occupy a large variety of habitats possess a diverse enzymatic machinery, while species with a specific habitat have a more focused enzyme repertoire that is well-adapted to the prevailing substrate. White-rot basidiomycete fungi also live in a specific habitat, as they are found exclusively in wood. In this study, we evaluated how well the enzymatic machinery of the white-rot fungus Dichomitus squalens is tailored to degrade its natural wood substrate. The transcriptome and exoproteome of D. squalens were analyzed after cultivation on two natural substrates, aspen and spruce wood, and two non-woody substrates, wheat bran and cotton seed hulls. D. squalens produced ligninolytic enzymes mainly at the early time point of the wood cultures, indicating the need to degrade lignin to get access to wood polysaccharides. Surprisingly, the response of the fungus to the non-woody polysaccharides was nearly as good a match to the substrate composition as observed for the wood polysaccharides. This indicates that D. squalens has preserved its ability to efficiently degrade plant biomass types not present in its natural habitat.
[Mh] Termos MeSH primário: Proteínas Fúngicas/genética
Polyporaceae/genética
Madeira/microbiologia
[Mh] Termos MeSH secundário: Biomassa
Proteínas Fúngicas/metabolismo
Lignina/metabolismo
Picea/metabolismo
Picea/microbiologia
Polyporaceae/crescimento & desenvolvimento
Polyporaceae/isolamento & purificação
Polyporaceae/metabolismo
Transcriptoma
Madeira/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungal Proteins); 9005-53-2 (Lignin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161229
[St] Status:MEDLINE
[do] DOI:10.1111/1462-2920.13652


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[PMID]:27793681
[Au] Autor:Wang SS; Ning YJ; Wang SN; Zhang J; Zhang GQ; Chen QJ
[Ad] Endereço:Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, College of Biological Science and Engineering, Beijing University of Agriculture, Beijing, 102206, China; College of Plant Science and Technology, Beijing University of Agriculture, Beijing, 102206, China.
[Ti] Título:Purification, characterization, and cloning of an extracellular laccase with potent dye decolorizing ability from white rot fungus Cerrena unicolor GSM-01.
[So] Source:Int J Biol Macromol;95:920-927, 2017 Feb.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A novel laccase was purified from fermentation broth of the white rot fungus Cerrena unicolor strain GSM-01 following three ion-exchange chromatography steps and one gel-filtration step. The purified enzyme was determined to be a monomeric protein of 63.2kDa and demonstrated high oxidation activity of 2.05×10 U/mg towards ABTS. Its cDNA, gene, and amino acid sequences were obtained. It possessed high sequence similarity with that of other laccases but different enzymatic properties. It manifested optimal pH and temperature of 2.6 and 45°C, respectively. Fe and Fe were the most efficient inhibitors towards Cerrena unicolor laccase (CUL), while Mn can slightly enhance the laccase activity of 3.8-10.5%. The K and V of CUL were estimated to 302.7µM and 13.6µMm , respectively. CUL was effective in the decolorization of bromothymol blue, evans blue, methyl orange, and malachite green with decolorization efficiencies of 50%-85%. It possesses potential application in textile and environmental industries.
[Mh] Termos MeSH primário: Corantes/metabolismo
Espaço Extracelular/enzimologia
Lacase/genética
Lacase/metabolismo
Polyporaceae/citologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Clonagem Molecular
Cor
Espaço Extracelular/genética
Concentração de Íons de Hidrogênio
Cinética
Lacase/química
Lacase/isolamento & purificação
Peso Molecular
Polyporaceae/enzimologia
Especificidade por Substrato
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Coloring Agents); EC 1.10.3.2 (Laccase)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170405
[Lr] Data última revisão:
170405
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161030
[St] Status:MEDLINE


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[PMID]:28094747
[Au] Autor:Nyam KL; Chang CY; Tan CS; Ng ST
[Ad] Endereço:Department of Food Science with Nutrition, Faculty of Applied Sciences, UCSI University, Cheras, Kuala Lumpur, Malaysia.
[Ti] Título:Investigation of the Tiger Milk Medicinal Mushroom, Lignosus rhinocerotis (Agaricomycetes), as an Antiulcer Agent.
[So] Source:Int J Med Mushrooms;18(12):1093-1104, 2016.
[Is] ISSN:1940-4344
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to determine the antiulcer activity of Lignosus rhinocerotis in rats. A total of 48 Sprague-Dawley rats were used in ethanol-induced, aspirin-induced, and water immersion-restraint stress-induced ulcer models. Rats were equally divided into 4 groups for each model and orally administered 5 mL/kg distilled water, 20 mg/kg omeprazole, as well as 250 and 500 mg/kg of L. rhinocerotis powder. L. rhinocerotis powder at both 250 and 500 mg/kg doses demonstrated significant (P < 0.05) protection against gastric ulceration in all the induced ulcer models. Histological studies revealed severe damage and hemorrhage of gastric mucosa in the negative control group for all ulcer-induced models. The study suggests that L. rhinocerotis powder possesses dose-dependent antiulcer activity in the gastric mucosa, as ascertained grossly and histologically, compared with the negative control groups.
[Mh] Termos MeSH primário: Antiulcerosos/administração & dosagem
Dieta/métodos
Polyporaceae
Úlcera Gástrica/prevenção & controle
[Mh] Termos MeSH secundário: Administração Oral
Animais
Masculino
Ratos Sprague-Dawley
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Ulcer Agents)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170308
[Lr] Data última revisão:
170308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170118
[St] Status:MEDLINE
[do] DOI:10.1615/IntJMedMushrooms.v18.i12.40


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[PMID]:27527131
[Au] Autor:Yang J; Xu X; Ng TB; Lin J; Ye X
[Ad] Endereço:Fujian Key Laboratory of Marine Enzyme Engineering, Fuzhou University, Fuzhou 350116, Fujian, China. T09136@fzu.edu.cn.
[Ti] Título:Laccase Gene Family in Cerrena sp. HYB07: Sequences, Heterologous Expression and Transcriptional Analysis.
[So] Source:Molecules;21(8), 2016 Aug 04.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Laccases are a class of multi-copper oxidases with industrial potential. In this study, eight laccases (Lac1-8) from Cerrena sp. strain HYB07, a white-rot fungus with high laccase yields, were analyzed. The laccases showed moderate identities to each other as well as with other fungal laccases and were predicted to have high redox potentials except for Lac6. Selected laccase isozymes were heterologously expressed in the yeast Pichia pastoris, and different enzymatic properties were observed. Transcription of the eight laccase genes was differentially regulated during submerged and solid state fermentation, as shown by quantitative real-time polymerase chain reaction and validated reference genes. During 6-day submerged fermentation, Lac7 and 2 were successively the predominantly expressed laccase gene, accounting for over 95% of all laccase transcripts. Interestingly, accompanying Lac7 downregulation, Lac2 transcription was drastically upregulated on days 3 and 5 to 9958-fold of the level on day 1. Consistent with high mRNA abundance, Lac2 and 7, but not other laccases, were identified in the fermentation broth by LC-MS/MS. In solid state fermentation, less dramatic differences in transcript abundance were observed, and Lac3, 7 and 8 were more highly expressed than other laccase genes. Elucidating the properties and expression profiles of the laccase gene family will facilitate understanding, production and commercialization of the fungal strain and its laccases.
[Mh] Termos MeSH primário: Lacase/genética
Lacase/metabolismo
Polyporaceae/enzimologia
Análise de Sequência de DNA/métodos
[Mh] Termos MeSH secundário: Domínio Catalítico
Clonagem Molecular
Fermentação
Proteínas Fúngicas/química
Proteínas Fúngicas/genética
Proteínas Fúngicas/metabolismo
Regulação Enzimológica da Expressão Gênica
Regulação Fúngica da Expressão Gênica
Lacase/química
Modelos Moleculares
Simulação de Acoplamento Molecular
Família Multigênica
Polyporaceae/química
Polyporaceae/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungal Proteins); EC 1.10.3.2 (Laccase)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170417
[Lr] Data última revisão:
170417
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160817
[St] Status:MEDLINE


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[PMID]:27316978
[Au] Autor:Quang DN; Wagner C; Merzweiler K; Abate D; Porzel A; Schmidt J; Arnold N
[Ad] Endereço:Leibniz Institute of Plant Biochemistry, Department of Bioorganic Chemistry, Weinberg 3, D-06120 Halle (Saale), Germany; Faculty of Chemistry, Hanoi National University of Education, 136 Xuan Thuy Road, Cau Giay, Hanoi, Vietnam.
[Ti] Título:Pyrofomins A-D, polyoxygenated sesquiterpenoids from Pyrofomes demidoffii.
[So] Source:Fitoterapia;112:229-32, 2016 Jul.
[Is] ISSN:1873-6971
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Pyrofomins A-D, four polyoxygenated sesquiterpenoids have been isolated from the methanolic extract of the fruit bodies of Pyrofomes demidoffii. Their structures are elucidated by IR, HR-FTICR-MS, and 2D NMR spectroscopy. Furthermore, the cedrane carbon skeleton of pyrofomin A (1) is confirmed by X-ray crystallographic analysis. The sesquiterpenoids 1-4 show neither cytotoxicity against KB cells nor antimicrobial activity.
[Mh] Termos MeSH primário: Polyporaceae/química
Sesquiterpenos/química
[Mh] Termos MeSH secundário: Linhagem Celular Tumoral
Carpóforos/química
Seres Humanos
Testes de Sensibilidade Microbiana
Estrutura Molecular
Sesquiterpenos/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Sesquiterpenes); 0 (pyrofomin A)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170213
[Lr] Data última revisão:
170213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160619
[St] Status:MEDLINE


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[PMID]:27279538
[Au] Autor:Yang J; Lin Q; Lin J; Ye X
[Ad] Endereço:College of Biological Sciences and Technology, Fuzhou University, Fuzhou, Fujian, China; Fujian Key Laboratory of Marine Enzyme Engineering, Fuzhou, Fujian, China.
[Ti] Título:Selection and Validation of Reference Genes for Quantitative Real-Time Polymerase Chain Reaction Studies in Mossy Maze Polypore, Cerrena unicolor (Higher Basidiomycetes).
[So] Source:Int J Med Mushrooms;18(2):165-75, 2016.
[Is] ISSN:1940-4344
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:With its ability to produce ligninolytic enzymes such as laccases, white-rot basidiomycete Cerrena unicolor, a medicinal mushroom, has great potential in biotechnology. Elucidation of the expression profiles of genes encoding ligninolytic enzymes are important for increasing their production. Quantitative real-time polymerase chain reaction (qPCR) is a powerful tool to study transcriptional regulation of genes of interest. To ensure accuracy and reliability of qPCR analysis of C. unicolor, expression levels of seven candidate reference genes were studied at different growth phases, under various induction conditions, and with a range of carbon/nitrogen ratios and carbon and nitrogen sources. The stability of the genes were analyzed with five statistical approaches, namely geNorm, NormFinder, BestKeeper, the ΔCt method, and RefFinder. Our results indicated that the selection of reference genes varied with sample sets. A combination of four reference genes (Cyt-c, ATP6, TEF1, and ß-tubulin) were recommended for normalizing gene expression at different growth phases. GAPDH and Cyt-c were the appropriate reference genes under different induction conditions. ATP6 and TEF1 were most stable in fermentation media with various carbon/nitrogen ratios. In the fermentation media with various carbon or nitrogen sources, 18S rRNA and GAPDH were the references of choice. The present study represents the first validation analysis of reference genes in C. unicolor and serves as a foundation for its qPCR analysis.
[Mh] Termos MeSH primário: Perfilação da Expressão Gênica/métodos
Perfilação da Expressão Gênica/normas
Genes Fúngicos
Polyporaceae/genética
Reação em Cadeia da Polimerase em Tempo Real/métodos
Reação em Cadeia da Polimerase em Tempo Real/normas
Padrões de Referência
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170308
[Lr] Data última revisão:
170308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160610
[St] Status:MEDLINE
[do] DOI:10.1615/IntJMedMushrooms.v18.i2.70


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[PMID]:27279536
[Au] Autor:Keong CY; B V; Daker M; Hamzah MY; Mohamad SA; Lan J; Chen X; Yang YP
[Ad] Endereço:Phytochemistry Unit, Herbal Medicine Research Centre, Institute for Medical Research, Jalan Pahang, Kuala Lumpur, Malaysia.
[Ti] Título:Fractionation and Biological Activities of Water-Soluble Polysaccharides from Sclerotium of Tiger Milk Medicinal Mushroom, Lignosus rhinocerotis (Agaricomycetes).
[So] Source:Int J Med Mushrooms;18(2):141-54, 2016.
[Is] ISSN:1940-4344
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This study investigated antioxidant and anti-inflammatory properties, and the direct cytotoxic effect of Lignosus rhinocerotis fractions, especially the polysaccharide fraction, on nasopharyngeal carcinoma cells. L. rhinocerotis crude extract was obtained through hot water extraction. The precipitate saturated with 30% ammonium sulfate was purified with ion-exchanged chromatography. Gel permeation chromatography multiangle laser light scattering analysis equipped with light scattering and UV signals revealed two district groups of polymers. A total of four peaks were observed in the total carbohydrate test. Fraction C, which was the second region of the second peak eluted with 0.3 M NaOH, showed the highest integrated molecular weight, whereas fraction E had the lowest integrated molecular weight of 19,790 Da. Fraction A contained the highest ß-D-glucan content. Enzymatic analysis showed that most of the polysaccharide fractions contained ß-1-3 and ß-1-6 skeletal backbones. The peak eluted with 0.6 M NaOH was separated in fraction D (flask 89-92) and fraction E (93-96). The results showed that fraction E expressed higher antioxidant activities than fraction D whereas fraction D expressed higher chelating activity than fraction E. The extract saturated with 30% ammonium sulfate exhibited higher reducing power than the extract saturated with 100% ammonium sulfate. Fractions D and E significantly inhibited the secretion of tumor necrosis factor-α in lipopolysaccharide-stimulated RAW 264.7 macrophages in a dose-dependent manner. There was no apparent difference in the viability of cells exposed or unexposed to L. rhinocerotis fractions.
[Mh] Termos MeSH primário: Anti-Inflamatórios/metabolismo
Antioxidantes/metabolismo
Polyporaceae/química
Polissacarídeos/metabolismo
[Mh] Termos MeSH secundário: Animais
Anti-Inflamatórios/isolamento & purificação
Antioxidantes/isolamento & purificação
Linhagem Celular Tumoral
Sobrevivência Celular/efeitos dos fármacos
Precipitação Química
Cromatografia em Gel
Citocinas/secreção
Seres Humanos
Macrófagos/efeitos dos fármacos
Camundongos
Peso Molecular
Polissacarídeos/química
Polissacarídeos/isolamento & purificação
Células RAW 264.7
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Antioxidants); 0 (Cytokines); 0 (Polysaccharides)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170308
[Lr] Data última revisão:
170308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160610
[St] Status:MEDLINE
[do] DOI:10.1615/IntJMedMushrooms.v18.i2.50



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