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[PMID]: 28463479 [Au] Autor: Hong CY; Ryu SH; Jeong H; Lee SS; Kim M; Choi IG [Ad] Endereço: Division of Wood Chemistry & Microbiology, Department of Forest Products, National Institute of Forest Science , Seoul, Republic of Korea. [Ti] Título: Phanerochaete chrysosporium Multienzyme Catabolic System for in Vivo Modification of Synthetic Lignin to Succinic Acid. [So] Source: ACS Chem Biol;12(7):1749-1759, 2017 Jul 21. [Is] ISSN: 1554-8937 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Whole cells of the basidiomycete fungus Phanerochaete chrysosporium (ATCC 20696) were applied to induce the biomodification of lignin in an in vivo system. Our results indicated that P. chrysosporium has a catabolic system that induces characteristic biomodifications of synthetic lignin through a series of redox reactions, leading not only to the degradation of lignin but also to its polymerization. The reducing agents ascorbic acid and α-tocopherol were used to stabilize the free radicals generated from the ligninolytic process. The application of P. chrysosporium in combination with reducing agents produced aromatic compounds and succinic acid as well as degraded lignin polymers. P. chrysosporium selectively catalyzed the conversion of lignin to succinic acid, which has an economic value. A transcriptomic analysis of P. chrysosporium suggested that the bond cleavage of synthetic lignin was caused by numerous enzymes, including extracellular enzymes such as lignin peroxidase and manganese peroxidase, and that the aromatic compounds released were metabolized in both the short-cut and classical tricarboxylic acid cycles of P. chrysosporium. In conclusion, P. chrysosporium is suitable as a biocatalyst for lignin degradation to produce a value-added product. [Mh] Termos MeSH primário: Lignina/metabolismo Complexos Multienzimáticos/química Phanerochaete/enzimologia Ácido Succínico/síntese química [Mh] Termos MeSH secundário: Ácido Ascórbico/química Radicais Livres Lignina/química Peso Molecular Nitrobenzenos/química Oxirredução Phanerochaete/metabolismo Ácido Succínico/química Ácido Succínico/metabolismo Tocoferóis/química [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Free Radicals); 0 (Multienzyme Complexes); 0 (Nitrobenzenes); 9005-53-2 (Lignin); AB6MNQ6J6L (Succinic Acid); E57JCN6SSY (nitrobenzene); PQ6CK8PD0R (Ascorbic Acid); R0ZB2556P8 (Tocopherols) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180207 [Lr] Data última revisão: 180207 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170503 [St] Status: MEDLINE [do] DOI: 10.1021/acschembio.7b00046

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[PMID]: 28683381 [Au] Autor: Huang C; Zeng G; Huang D; Lai C; Xu P; Zhang C; Cheng M; Wan J; Hu L; Zhang Y [Ad] Endereço: College of Environmental Science and Engineering, Hunan University, Changsha 410082, China; Key Laboratory of Environmental Biology and Pollution Control, Ministry of Education, Hunan University, Changsha 410082, China. [Ti] Título: Effect of Phanerochaete chrysosporium inoculation on bacterial community and metal stabilization in lead-contaminated agricultural waste composting. [So] Source: Bioresour Technol;243:294-303, 2017 Nov. [Is] ISSN: 1873-2976 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: The effects of Phanerochaete chrysosporium inoculation on bacterial community and lead (Pb) stabilization in composting of Pb-contaminated agricultural waste were studied. It was found that the bioavailable Pb was transformed to stable Pb after composting with inoculum of P. chrysosporium. Pearson correlation analysis revealed that total organic carbon (TOC) and carbon/nitrogen (C/N) ratio significantly (P<0.05) influenced the distribution of Pb fractions. The richness and diversity of bacterial community were reduced under Pb stress and increased after inoculation with P. chrysosporium. Redundancy analysis indicated that C/N ratio, total organic matter, temperature and soluble-exchangeable Pb were the significant parameters to affect the bacterial community structure, solely explained 14.7%, 11.1%, 10.4% and 8.3% of the variation in bacterial community composition, respectively. In addition, the main bacterial species, being related to organic matter degradation and Pb stabilization, were found. These findings will provide useful information for composting of heavy metal-contaminated organic wastes. [Mh] Termos MeSH primário: Agricultura Phanerochaete Microbiologia do Solo [Mh] Termos MeSH secundário: Chumbo Solo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Soil); 2P299V784P (Lead) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171116 [Lr] Data última revisão: 171116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170707 [St] Status: MEDLINE

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[PMID]: 28662549 [Au] Autor: Hu L; Liu Y; Zeng G; Chen G; Wan J; Zeng Y; Wang L; Wu H; Xu P; Zhang C; Cheng M; Hu T [Ad] Endereço: College of Environmental Science and Engineering, Hunan University, Changsha, Hunan, 410082, PR China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha, Hunan, 410082, PR China. [Ti] Título: Organic matters removal from landfill leachate by immobilized Phanerochaete chrysosporium loaded with graphitic carbon nitride under visible light irradiation. [So] Source: Chemosphere;184:1071-1079, 2017 Oct. [Is] ISSN: 1879-1298 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: This study investigated the technical applicability of a combination of Phanerochaete chrysosporium (P. chrysosporium) with photocatalyst graphitic carbon nitride (g-C N ) for organic matters removal from landfill leachate under visible light irradiation. Photocatalyst g-C N was well immobilized on the hyphae surface of P. chrysosporium by calcium alginate. The typical absorption edge in visible light region for g-C N was at about 460 nm, and the optical absorption bandgap of g-C N was estimated to be 2.70 eV, demonstrating the great photoresponsive ability of g-C N . An optimized g-C N content of 0.10 g in immobilized P. chrysosporium and an optimized immobilized P. chrysosporium dosage of 1.0 g were suitable for organic matters removal. The removal efficiency of total organic carbon (TOC) reached 74.99% in 72 h with the initial TOC concentration of 100 mg L . In addition, the gas chromatography coupled with mass spectrometry (GC-MS) measurements showed that immobilized P. chrysosporium presented an outstanding removal performance for almost all organic compounds in landfill leachate, especially for the volatile fatty acids and long-chain hydrocarbons. The overall results indicate that the combination P. chrysosporium with photocatalyst g-C N for organic matters removal from landfill leachate may provide a more comprehensive potential for the landfill leachate treatment. [Mh] Termos MeSH primário: Grafite/química Nitrilos/química Compostos Orgânicos/análise Phanerochaete/química Eliminação de Resíduos Líquidos/métodos Poluentes Químicos da Água/análise [Mh] Termos MeSH secundário: Alginatos Catálise Ácido Glucurônico Ácidos Hexurônicos Luz Compostos Orgânicos/química Poluentes Químicos da Água/química [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Alginates); 0 (Hexuronic Acids); 0 (Nitriles); 0 (Organic Chemicals); 0 (Water Pollutants, Chemical); 534Q0F66RK (cyanogen); 7782-42-5 (Graphite); 8A5D83Q4RW (Glucuronic Acid); 8C3Z4148WZ (alginic acid) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 170929 [Lr] Data última revisão: 170929 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170701 [St] Status: MEDLINE

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[PMID]: 28193432 [Au] Autor: da Silva RR; de Oliveira LC; Juliano MA; Juliano L; de Oliveira AH; Rosa JC; Cabral H [Ad] Endereço: Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil. [Ti] Título: Biochemical and milk-clotting properties and mapping of catalytic subsites of an extracellular aspartic peptidase from basidiomycete fungus Phanerochaete chrysosporium. [So] Source: Food Chem;225:45-54, 2017 Jun 15. [Is] ISSN: 0308-8146 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: For a long time, proteolytic enzymes have been employed as key tools of industrial processes, especially in the dairy industry. In the present work, we used Phanerochaete chrysosporium for biochemical characterization and analysis of catalytic specificity of an aspartic peptidase. Our results revealed an aspartic peptidase with molecular mass ∼38kDa, maximal activity at pH 4.5 and 50°C, and stability above 80% in the pH range of 3-8 and temperature up to 55°C for 1h. In a milk-clotting assay, this peptidase showed maximal milk clotting activity at 60-65°C and maintenance of enzymatic activity above 80% in the presence of 20mM CaCl . In a specificity assay, we observed stronger restriction of catalysis at the S subsite, with a preference for lysine, arginine, leucine, tyrosine, and phenylalanine residues. The restricted proteolysis and milk-clotting potential are attractive properties for the use in cheese production. [Mh] Termos MeSH primário: Ácido Aspártico Proteases/metabolismo Queijo/microbiologia Indústria de Processamento de Alimentos Leite/microbiologia Phanerochaete/enzimologia [Mh] Termos MeSH secundário: Animais Proteínas Fúngicas/metabolismo Temperatura Ambiente [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Fungal Proteins); EC 3.4.- (Aspartic Acid Proteases) [Em] Mês de entrada: 1705 [Cu] Atualização por classe: 170504 [Lr] Data última revisão: 170504 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170215 [St] Status: MEDLINE

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[PMID]: 28183273 [Au] Autor: Watanabe N; Ohkusu K; Okuda M; Imataki O; Ishii T; Negayama K; Tadokoro A; Kita N; Takagi T; Kanaji N; Kadowaki N; Bandoh S [Ad] Endereço: Department of Internal Medicine, Hematology, Rheumatology and Respiratory Medicine, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa, 761-0793, Japan. naoki-w@med.kagawa-u.ac.jp. [Ti] Título: Phanerochaete sordida as a cause of pulmonary nodule in an immunocompromised patient: a case report. [So] Source: BMC Infect Dis;17(1):135, 2017 Feb 10. [Is] ISSN: 1471-2334 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: BACKGROUND: Phanerochaete sordida is a species of wood rotting fungus, which can degrade lignin, cellulose and hemicellulose contained in wood and other hard-to-biodegrade organic substances. However, to date, there have been no other reports demonstrating that P. sordida can infect humans. CASE PRESENTATION: A 66-year-old Japanese man presented for a mass increasing in size on his left thigh. He had been suffering from rheumatoid arthritis for 18 years and chronic obstructive pulmonary disease for 20 years, for which he was being treated with 5 mg/day prednisolone and 8 mg/week methotrexate. The mass resection was performed two months later, and was diagnosed as malignant fibrous histiocytosis. However, a computed tomography examination for tumor recurrence after surgery showed a newly emergent pulmonary nodule. We therefore decided to resect the nodule by thoracoscopic procedure. Histopathological examination of the excised specimen showed that the lesion was a granuloma, with necrotic tissue and clumping of Aspergillus-like hyphae. Therefore, the nodule was diagnosed as a fungal infection and tissue specimens were cultured microbiologically. However, fungal growth was not observed. We consequently performed genetic analysis using a broad-range polymerase chain reaction. The 28S rRNA sequence demonstrated 100% homology with P. sordida using the NCBI BLAST program against the GenBank DNA databases. CONCLUSIONS: Using broad-range polymerase chain reaction, we identified P. sordida as the causative agent of a pulmonary nodule. These findings indicate that P. sordida may be an additional opportunistic causative organism of pulmonary infection in immunocompromised patients. [Mh] Termos MeSH primário: Hospedeiro Imunocomprometido Pneumopatias Fúngicas/diagnóstico Phanerochaete/isolamento & purificação [Mh] Termos MeSH secundário: Idoso Artrite Reumatoide DNA Fúngico/análise Diagnóstico Diferencial Seres Humanos Pneumopatias Fúngicas/diagnóstico por imagem Pneumopatias Fúngicas/microbiologia Pneumopatias Fúngicas/cirurgia Masculino Phanerochaete/genética Reação em Cadeia da Polimerase Doença Pulmonar Obstrutiva Crônica [Pt] Tipo de publicação: CASE REPORTS; JOURNAL ARTICLE [Nm] Nome de substância: 0 (DNA, Fungal) [Em] Mês de entrada: 1707 [Cu] Atualização por classe: 170705 [Lr] Data última revisão: 170705 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170211 [St] Status: MEDLINE [do] DOI: 10.1186/s12879-017-2244-9

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[PMID]: 28125213 [Au] Autor: Vuong TV; Liu B; Sandgren M; Master ER [Ad] Endereço: Department of Chemical Engineering and Applied Chemistry, University of Toronto , 200 College Street, Toronto, Ontario M5S 3E5, Canada. [Ti] Título: Microplate-Based Detection of Lytic Polysaccharide Monooxygenase Activity by Fluorescence-Labeling of Insoluble Oxidized Products. [So] Source: Biomacromolecules;18(2):610-616, 2017 Feb 13. [Is] ISSN: 1526-4602 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Most existing methods for screening the activity of lytic polysaccharide mono-oxygenases (LPMOs) on polysaccharides are based on the detection of soluble oxidized sugars. This approach might underestimate the total performance of LPMOs since oxidation events that do not lead to oligosaccharide release are not detected. Using PcLPMO9D as a model enzyme, a microplate-based method has been developed to detect C1-oxidizing LPMO activity by covalently linking a water-soluble fluorophore to oxidized positions within the cellulose fiber. This fluorescence method was validated using X-ray photoelectron spectroscopy and then combined with high-performance anion-exchange chromatography to track total PcLPMO9D activity. [Mh] Termos MeSH primário: Fluorescência Microtecnologia/métodos Oxigenases de Função Mista/metabolismo Phanerochaete/enzimologia Polissacarídeos/química [Mh] Termos MeSH secundário: Celulose/química Quitina/química Oxirredução Phanerochaete/crescimento & desenvolvimento Espectroscopia Fotoeletrônica Especificidade por Substrato [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Polysaccharides); 1398-61-4 (Chitin); 9004-34-6 (Cellulose); EC 1.- (Mixed Function Oxygenases) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171011 [Lr] Data última revisão: 171011 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170127 [St] Status: MEDLINE [do] DOI: 10.1021/acs.biomac.6b01790

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[PMID]: 28123349 [Au] Autor: Kameshwar AK; Qin W [Ad] Endereço: Department of Biology, Lakehead University, 955 Oliver Road, Thunder Bay, Ontario, P7B 5E1, Canada. [Ti] Título: Metadata Analysis of Gene Expression Data Identified Common CAZymes Encoding Gene Expression Profiles Involved in Cellulose and Hemicellulose Degradation. [So] Source: Int J Biol Sci;13(1):85-99, 2017. [Is] ISSN: 1449-2288 [Cp] País de publicação: Australia [La] Idioma: eng [Ab] Resumo: In literature, extensive studies have been conducted on popular wood degrading white rot fungus, about its lignin degrading mechanisms compared to the cellulose and hemicellulose degrading abilities. This study delineates cellulose and hemicellulose degrading mechanisms through large scale metadata analysis of gene expression data (retrieved from NCBI GEO) to understand the common expression patterns of differentially expressed genes when cultured on different growth substrates. Genes encoding glycoside hydrolase classes commonly expressed during breakdown of cellulose such as GH-5,6,7,9,44,45,48 and hemicellulose are GH-2,8,10,11,26,30,43,47 were found to be highly expressed among varied growth conditions including simple customized and complex natural plant biomass growth mediums. Genes encoding carbohydrate esterase class enzymes CE (1,4,8,9,15,16) polysaccharide lyase class enzymes PL-8 and PL-14, and glycosyl transferases classes GT (1,2,4,8,15,20,35,39,48) were differentially expressed in natural plant biomass growth mediums. Based on these results, on natural plant biomass substrates was found to express lignin and hemicellulose degrading enzymes more than cellulolytic enzymes except GH-61 (LPMO) class enzymes, in early stages. It was observed that the fate of transcriptome is significantly affected by the wood substrate provided. We believe, the gene expression findings in this study plays crucial role in developing genetically efficient microbe with effective cellulose and hemicellulose degradation abilities. [Mh] Termos MeSH primário: Celulose/metabolismo Proteínas Fúngicas/metabolismo Phanerochaete/enzimologia Phanerochaete/metabolismo Polissacarídeos/metabolismo [Mh] Termos MeSH secundário: Proteínas Fúngicas/genética Metadados Phanerochaete/genética Transcriptoma/genética [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Fungal Proteins); 0 (Polysaccharides); 8024-50-8 (hemicellulose); 9004-34-6 (Cellulose) [Em] Mês de entrada: 1711 [Cu] Atualização por classe: 171107 [Lr] Data última revisão: 171107 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170127 [St] Status: MEDLINE [do] DOI: 10.7150/ijbs.17390

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[PMID]: 28065548 [Au] Autor: Voberková S; Vaverková MD; Buresová A; Adamcová D; Vrsanská M; Kynický J; Brtnický M; Adam V [Ad] Endereço: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelská 1/1665, 613 00, Czech Republic. Electronic address: stanislava.voberkova@mendelu.cz. [Ti] Título: Effect of inoculation with white-rot fungi and fungal consortium on the composting efficiency of municipal solid waste. [So] Source: Waste Manag;61:157-164, 2017 Mar. [Is] ISSN: 1879-2456 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: An investigation was carried out on the effect of inoculation methods on the compost of an organic fraction of municipal solid waste. Three types of white-rot fungi (Phanerochaete chrysosporium, Trametes versicolor and Fomes fomentarius), and a consortium of these fungi, were used. The study assessed their influence on microbial enzymatic activities and the quality of the finished compost. It was found that the addition of white-rot fungi to municipal solid waste (after 37days of composting) could be a useful strategy for enhancing the properties of the final compost product. In comparison with the control sample (compost without inoculation), it accelerates degradation of solid waste as indicated by changes in C/N, electrical conductivity and pH. However, the effectiveness of waste degradation and compost maturation depends on the type of microorganism used for inoculation. The presence of inoculants, such as Trametes versicolor and Fomes fomentarius, led to a higher degrading ratio and a better degree of maturity. This resulted in an increase of enzymatic activities (especially dehydrogenase and protease) and a germination index in comparison with inoculation using Phanerochaete chrysosporium or a consortium of fungi. [Mh] Termos MeSH primário: Fungos/metabolismo Eliminação de Resíduos/métodos Microbiologia do Solo Solo Resíduos Sólidos [Mh] Termos MeSH secundário: Biodegradação Ambiental Carbono/metabolismo Coriolaceae/metabolismo Enzimas/metabolismo Concentração de Íons de Hidrogênio Nitrogênio/metabolismo Phanerochaete/metabolismo Trametes/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Enzymes); 0 (Soil); 0 (Solid Waste); 7440-44-0 (Carbon); N762921K75 (Nitrogen) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 171116 [Lr] Data última revisão: 171116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170110 [St] Status: MEDLINE

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[PMID]: 27923672 [Au] Autor: Barraco-Vega M; Romero H; Richero M; Cerdeiras MP; Cecchetto G [Ad] Endereço: Microbiología Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo 11800, Uruguay. Electronic address: mariveba@gmail.com. [Ti] Título: Functional characterization of two novel purine transporters from the Basidiomycota Phanerochaete chrysosporium. [So] Source: Gene;601:1-10, 2017 Feb 15. [Is] ISSN: 1879-0038 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: Purine transporters as substrate entry points in organisms, are involved in a number of cellular processes such as nitrogen source uptake, energy metabolism and synthesis of nucleic acids. In this study, two nucleobase transporter genes (phZ, phU) from Phanerochaete chrysosporium were cloned, identified, and functionally characterized. Our results show that PhZ is a transporter of adenine and hypoxanthine, and a protein belonging to the AzgA-like family, whilst PhU belongs to the NAT/NCS2 family, transporting xanthine and uric acid. No other sequences belonging to these families were detected in P. chrysosporium's genome. Phylogenetic analyses show that AzgA-like sequences form monophyletic groups for each major lineage (Ascomycota, Basidiomycota and Zygomycota). In contrast, Ascomycota and Basidiomycota NAT/NCS2 sequences do not form monophyletic groups and several copies of this protein are distributed across the tree. Expression of phU was significantly downregulated in the presence of a primary source like ammonium, and enhanced if purines were present or if the mycelium was nitrogen starved. phZ was clearly induced by its substrates (hypoxanthine, adenine), very lightly induced by xanthine, suppressed by urea and amino acids and expressed at a basal level when uric acid or ammonium was the nitrogen source or when the mycelium was starved for nitrogen. In order to perform substrate analyses, both P. chrysosporium proteins (PhZ, PhU) were expressed in Aspergillus nidulans. Epifluorescent microscopy showed that under inducing conditions, PhZ-GFP and PhU-GFP were present at the plasma membrane of A. nidulans transformed strains, and were internalized in repressed conditions. Our results suggest that in the white-rot fungus P. chrysosporium, phU has a catabolic role and phZ, (less dependent of the nitrogen source), plays a key role in purine acquisition to provide biosynthetic components. These are the first purine transporters characterized in Basidiomycota. [Mh] Termos MeSH primário: Proteínas Fúngicas/genética Proteínas Fúngicas/metabolismo Proteínas de Membrana Transportadoras/genética Proteínas de Membrana Transportadoras/metabolismo Phanerochaete/genética Phanerochaete/metabolismo Purinas/metabolismo [Mh] Termos MeSH secundário: Aspergillus nidulans/genética Aspergillus nidulans/metabolismo Clonagem Molecular Expressão Gênica Genes Fúngicos Filogenia Proteínas Recombinantes de Fusão/genética Proteínas Recombinantes de Fusão/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Fungal Proteins); 0 (Membrane Transport Proteins); 0 (Purines); 0 (Recombinant Fusion Proteins) [Em] Mês de entrada: 1701 [Cu] Atualização por classe: 170130 [Lr] Data última revisão: 170130 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161208 [St] Status: MEDLINE

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[PMID]: 27831926 [Au] Autor: Aracagök YD; Göker H; Cihangir N [Ad] Endereço: Department of Biology, Faculty of Science, Hacettepe University, 06800 Beytepe, Ankara. [Ti] Título: Biodegradation of micropollutant naproxen with a selected fungal strain and identification of metabolites. [So] Source: Z Naturforsch C;72(5-6):173-179, 2017 May 01. [Is] ISSN: 0939-5075 [Cp] País de publicação: Germany [La] Idioma: eng [Ab] Resumo: Pharmaceuticals are widely used for treating human and animal diseases. Naproxen [(S) 6-methoxy-α-methyl-2-naphthalene acetic acid] and its sodium salt are members of the α-arylpropionic acid group of nonsteroidal anti-inflammatory drugs. Due to excessive usage of naproxen, this drug has been determined even in drinking water. In this study, four fungal strains Phanerochaete chrysosporium, Funalia trogii, Aspergillus niger, and Yarrowia lipolytica were investigated in terms of naproxen removal abilities. According to LC/MS data, A. niger was found the most efficient strain with 98% removal rate. Two main by-products of fungal transformation, O-desmethylnaproxen and 7-hydroxynaproxen, were identified by using LC/MS, 1HNMR, and 13CNMR. Our results showed that O-demethylation and hydroxylation of naproxen is catalyzed by cytochrome P450 enzyme system. [Mh] Termos MeSH primário: Anti-Inflamatórios não Esteroides/metabolismo Aspergillus niger/enzimologia Sistema Enzimático do Citocromo P-450/metabolismo Proteínas Fúngicas/metabolismo Naproxeno/metabolismo Poluentes Químicos da Água/metabolismo Purificação da Água/métodos [Mh] Termos MeSH secundário: Anti-Inflamatórios não Esteroides/efeitos adversos Biodegradação Ambiental Biotransformação Espectroscopia de Ressonância Magnética Nuclear de Carbono-13 Cromatografia Líquida Hidroxilação Espectrometria de Massas Naproxeno/efeitos adversos Naproxeno/análogos & derivados Phanerochaete/enzimologia Espectroscopia de Prótons por Ressonância Magnética Poluentes Químicos da Água/efeitos adversos Yarrowia/enzimologia [Pt] Tipo de publicação: COMPARATIVE STUDY; JOURNAL ARTICLE [Nm] Nome de substância: 0 (Anti-Inflammatory Agents, Non-Steroidal); 0 (Fungal Proteins); 0 (Water Pollutants, Chemical); 57Y76R9ATQ (Naproxen); 9035-51-2 (Cytochrome P-450 Enzyme System); XSN14HHQ8D (desmethylnaproxen) [Em] Mês de entrada: 1711 [Cu] Atualização por classe: 171103 [Lr] Data última revisão: 171103 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161111 [St] Status: MEDLINE

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1			PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2
2	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2
3	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2

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