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Pesquisa : B01.300.300.300 [Categoria DeCS]
Referências encontradas : 236 [refinar]
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  1 / 236 MEDLINE  
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[PMID]:28672012
[Au] Autor:Zhang S; Chen C; Xie T; Ye S
[Ad] Endereço:China Jiliang University, Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, Hangzhou, China.
[Ti] Título:Identification and validation of reference genes for qRT-PCR studies of the obligate aphid pathogenic fungus Pandora neoaphidis during different developmental stages.
[So] Source:PLoS One;12(7):e0179930, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The selection of stable reference genes is a critical step for the accurate quantification of gene expression. To identify and validate the reference genes in Pandora neoaphidis-an obligate aphid pathogenic fungus-the expression of 13classical candidate reference genes were evaluated by quantitative real-time reverse transcriptase polymerase chain reaction(qPCR) at four developmental stages (conidia, conidia with germ tubes, short hyphae and elongated hyphae). Four statistical algorithms, including geNorm, NormFinder, BestKeeper and Delta Ct method were used to rank putative reference genes according to their expression stability and indicate the best reference gene or combination of reference genes for accurate normalization. The analysis of comprehensive ranking revealed that ACT1and 18Swas the most stably expressed genes throughout the developmental stages. To further validate the suitability of the reference genes identified in this study, the expression of cell division control protein 25 (CDC25) and Chitinase 1(CHI1) genes were used to further confirm the validated candidate reference genes. Our study presented the first systematic study of reference gene(s) selection for P. neoaphidis study and provided guidelines to obtain more accurate qPCR results for future developmental efforts.
[Mh] Termos MeSH primário: Afídeos/microbiologia
Entomophthorales/genética
Genes Fúngicos
[Mh] Termos MeSH secundário: Animais
Afídeos/genética
Entomophthorales/crescimento & desenvolvimento
Reação em Cadeia da Polimerase em Tempo Real
Padrões de Referência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171006
[Lr] Data última revisão:
171006
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170704
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179930


  2 / 236 MEDLINE  
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[PMID]:27993619
[Au] Autor:Malagocka J; Jensen AB; Eilenberg J
[Ad] Endereço:Centre for Social Evolution, Department of Plant and Environmental Science, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark. Electronic address: jopi@plen.ku.dk.
[Ti] Título:Pandora formicae, a specialist ant pathogenic fungus: New insights into biology and taxonomy.
[So] Source:J Invertebr Pathol;143:108-114, 2017 Feb.
[Is] ISSN:1096-0805
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Among fungi from the order Entomophthorales (Entomophthoromycota), there are many specialized, obligatory insect-killing pathogens. Pandora formicae (Humber & Balazy) Humber is a rare example of an entomophthoralean fungus adapted to exclusively infect social insects: wood ants from the genus Formica. There is limited information available on P. formicae; many important aspects of this host-pathogen system remain hitherto unknown, and the taxonomical status of the fungus is unclear. Our study fills out some main gaps in the life history of P. formicae, such as seasonal prevalence and overwintering strategy. Field studies of infection prevalence show a disease peak in late summer and early autumn. Typical thick-walled entomophthoralean resting spores of P. formicae are documented and described for the first time. The proportion of cadavers with resting spores increased from late summer throughout autumn, suggesting that these spores are the main overwintering fungal structures. In addition, the phylogenetic status of Pandora formicae is outlined. Finally, we review the available taxonomical literature and conclude that the name P. formicae should be used rather than the name P. myrmecophaga for ant-infecting fungi displaying described morphological features.
[Mh] Termos MeSH primário: Formigas/microbiologia
Entomophthorales/fisiologia
Interações Hospedeiro-Patógeno/fisiologia
[Mh] Termos MeSH secundário: Animais
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171118
[Lr] Data última revisão:
171118
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161221
[St] Status:MEDLINE


  3 / 236 MEDLINE  
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[PMID]:27637932
[Au] Autor:Hajek AE; Gryganskyi A; Bittner T; Liebherr JK; Liebherr JH; Jensen AB; Moulton JK; Humber RA
[Ad] Endereço:Department of Entomology, Cornell University, Ithaca, NY 14853-2601, USA. Electronic address: aeh4@cornell.edu.
[Ti] Título:Phylogenetic placement of two species known only from resting spores: Zoophthora independentia sp. nov. and Z. porteri comb nov. (Entomophthorales: Entomophthoraceae).
[So] Source:J Invertebr Pathol;140:68-74, 2016 Oct.
[Is] ISSN:1096-0805
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Molecular methods were used to determine the generic placement of two species of Entomophthorales known only from resting spores. Historically, these species would belong in the form-genus Tarichium, but this classification provides no information about phylogenetic relationships. Using DNA from resting spores, Zoophthora independentia, infecting Tipula (Lunatipula) submaculata in New York State, is now described as a new species and Tarichium porteri, described in 1942, which infects Tipula (Triplicitipula) colei in Tennessee, is transferred to the genus Zoophthora. We have shown that use of molecular methods can assist with determination of the phylogenetic relations of specimens within the form-genus Tarichium for an already described species and a new species for which only resting spores are available.
[Mh] Termos MeSH primário: Dípteros/parasitologia
Entomophthora/genética
Entomophthorales/genética
[Mh] Termos MeSH secundário: Animais
DNA Fúngico
Filogenia
Reação em Cadeia da Polimerase
Esporos Fúngicos
Zigomicose/veterinária
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Fungal)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160918
[St] Status:MEDLINE


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[PMID]:27486120
[Au] Autor:Torres Acosta RI; Humber RA; Sánchez-Peña SR
[Ad] Endereço:Departamento de Parasitología, Universidad Autónoma Agraria Antonio Narro, Saltillo, Coahuila C.P. 25315, Mexico. Electronic address: rita_taz84@hotmail.com.
[Ti] Título:Zoophthora radicans (Entomophthorales), a fungal pathogen of Bagrada hilaris and Bactericera cockerelli (Hemiptera: Pentatomidae and Triozidae): Prevalence, pathogenicity, and interplay of environmental influence, morphology, and sequence data on fungal identification.
[So] Source:J Invertebr Pathol;139:82-91, 2016 Sep.
[Is] ISSN:1096-0805
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The exotic bagrada bug or painted bug, Bagrada hilaris, and the native potato/tomato psyllid, Bactericera (=Paratrioza) cockerelli, are key pests of horticulture in western North America. In 2014-2015, adult and juvenile B. hilaris and B. cockerelli killed by fungi in the genus Zoophthora were detected near Saltillo, northeastern Mexico. We report the field prevalence and observations of Zoophthora on these hosts. The morphology and growth characteristics of field-collected specimens and pure in vitro cultures, as well as molecular markers (ITS1 and ITS4) were analyzed to identify these Zoophthora populations. Although there were morphological spore differences detected among field collections from both insect hosts, the fungi causing these mycoses can be identified as the same species (Zoophthora radicans), according to morphometric data from in vitro cultures (where differences observed in field material were attenuated) and sequence data (96-99% identity for ITS1 and 4). These results underscore the plasticity of field collections and in vitro cultures, and the relevance of comprehensive morphological and molecular analysis from cultures under standard conditions. Dose-response bioassays were conducted with one Z. radicans strain against bagrada bug nymphs. Exposure to conidial showers from cultures induced 30-90% mortality. This is the first report of a natural enemy of bagrada bug in Mexico, and the first published report of entomophthoralean fungi naturally attacking bagrada bugs and potato psyllids. Z. radicans should be further investigated as a tool in the biological control of hemipterans.
[Mh] Termos MeSH primário: Hemípteros/parasitologia
Controle Biológico de Vetores/métodos
Zigomicose/veterinária
[Mh] Termos MeSH secundário: Animais
Entomophthorales/patogenicidade
México
Prevalência
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160804
[St] Status:MEDLINE


  5 / 236 MEDLINE  
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[PMID]:27449676
[Au] Autor:Jackson D; Zemenick AT; Malloure B; Quandt CA; James TY
[Ad] Endereço:Department of Ecology and Evolutionary Biology, University of Michigan, 2019 Kraus Nat. Sci. Bldg., 830 North University, Ann Arbor, MI 48109, USA. Electronic address: dougjack@dougjackson.net.
[Ti] Título:Fine-scale spatial genetic structure of a fungal parasite of coffee scale insects.
[So] Source:J Invertebr Pathol;139:34-41, 2016 Sep.
[Is] ISSN:1096-0805
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The entomopathogenic fungus Lecanicillium lecanii persists in a highly dynamic network of habitat patches (i.e., a metapopulation) formed by its primary host, the green coffee scale Coccus viridis. Lecanicillium lecanii is an important biological control of both C. viridis and the coffee rust, Hemileia vastatrix. Successfully managing this biocontrol agent will depend on an increased understanding of the characteristics of its dispersal, as migration between occupied and unoccupied patches is essential for the persistence of this metapopulation. In the present study, we employ a population genetics approach, and show that in our study system, a coffee farm in the Soconusco region of southern Mexico, L. lecanii is characterized by clear spatial genetic structure among plots within the farm but a lack of apparent structure at smaller scales. This is consistent with dispersal dominated by highly localized transport, such as by insects or rain splash, and less dependence on longer distance dispersal such as wind transport. The study site was dominated by a few multi-locus microsatellite genotypes, and their identities and large-scale locations persist across both study years, suggesting that local epizootics (outbreaks) are initiated each wet season by residual propagules from the previous wet season, and not by long-distance transport of propagules from other sites. The index of association, a measure of linkage disequilibrium, indicates that epizootics are primarily driven by asexual, clonal reproduction, which is consistent with the apparent lack of a teleomorph in the study site and the presence of only a single mating type across the site (MAT-1-2-1). Although the same predominant clonal genotypes were found across years, a drastic difference in genotypic diversity was witnessed across two sites between the two years, suggesting that interclonal selection was occurring. In light of the dispersal limitation of L. lecanii, spatial structure may be an essential axis of management to ensure the persistence of L. lecanii and preserve the ecosystem services provided by this versatile biocontrol agent in this and similar coffee farms.
[Mh] Termos MeSH primário: Entomophthorales/genética
Hemípteros/parasitologia
[Mh] Termos MeSH secundário: Animais
Coffea
Fazendas
México
Controle Biológico de Vetores/métodos
Reação em Cadeia da Polimerase
Zigomicose/veterinária
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160725
[St] Status:MEDLINE


  6 / 236 MEDLINE  
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[PMID]:27053740
[Au] Autor:Hajek AE; van Nouhuys S
[Ad] Endereço:Department of Entomology, Cornell University, Ithaca, NY 14853-2601, USA aeh4@cornell.edu.
[Ti] Título:Fatal diseases and parasitoids: from competition to facilitation in a shared host.
[So] Source:Proc Biol Sci;283(1828), 2016 04 13.
[Is] ISSN:1471-2954
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Diverse parasite taxa share hosts both at the population level and within individual hosts, and their interactions, ranging from competitive exclusion to facilitation, can drive community structure and dynamics. Emergent pathogens have the potential to greatly alter community interactions. We found that an emergent fungal entomopathogen dominated pre-existing lethal parasites in populations of the forest defoliating gypsy moth,Lymantria dispar The parasite community was composed of the fungus and four parasitoid species that only develop successfully after they kill the host, and a virus that produces viable propagules before the host has died. A low-density site was sampled over 17 years and compared with 66 sites across a range of host densities, including outbreaks. The emergent fungal pathogen and competing parasitoids rarely co-infected host individuals because each taxa must kill its host. The virus was not present at low host densities, but successfully co-infected with all other parasite species. In fact, there was facilitation between the virus and one parasitoid species hosting a polydnavirus. This newly formed parasite community, altered by an emergent pathogen, is shaped both by parasite response to host density and relative abilities of parasites to co-inhabit the same host individuals.
[Mh] Termos MeSH primário: Dípteros/fisiologia
Entomophthorales/fisiologia
Mariposas/microbiologia
Mariposas/parasitologia
Nucleopolyhedrovirus/fisiologia
Vespas/fisiologia
[Mh] Termos MeSH secundário: Animais
Interações Hospedeiro-Parasita
Interações Hospedeiro-Patógeno
Larva/crescimento & desenvolvimento
Larva/microbiologia
Larva/parasitologia
Mariposas/crescimento & desenvolvimento
New York
Controle Biológico de Vetores
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170503
[Lr] Data última revisão:
170503
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160408
[St] Status:MEDLINE


  7 / 236 MEDLINE  
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[PMID]:26968351
[Au] Autor:Montalva C; Collier K; Luz C; Humber RA
[Ad] Endereço:Laboratório de Patologia de Invertebrados (LPI), Instituto de Patologia Tropical e Saúde Pública (IPTSP), Universidade Federal de Goiás (UFG), Goiânia, Goiás, Brazil.
[Ti] Título:Pandora bullata (Entomophthoromycota: Entomophthorales) affecting calliphorid flies in central Brazil.
[So] Source:Acta Trop;158:177-80, 2016 Jun.
[Is] ISSN:1873-6254
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Fungi are where one finds them, and if one seeks fungal pathogens affecting flies, then a garbage dump may be an ideal place to find both persistent, abundant fly populations and their fungal pathogens. An obvious fungal epizootic affecting the oriental latrine fly, Chrysomya megacephala (Diptera: Calliphoridae), was observed over several days in mid-February 2015 at the local garbage dump adjacent to the city of Cavalcante, northern Goiás. This site harbored large populations of both C. megacephala and a Musca sp. (Diptera: Muscidae) but only the population of oriental latrine fly was affected by any fungal pathogen and presented unusually dense populations of fresh cadavers. The fungus was identifiable as Pandora bullata (Entomophthorales: Entomophthoraceae) only after a very small number of characteristically decorated resting spores were found in these flies two months later; this represents the first Brazilian (and South American) record of this species. P. bullata is known previously from a small number of North American, European and Australian collections, all of which have included relatively abundant production of resting spores. We cannot dismiss the possibility that the extremely sparse formation of resting spores at this Brazilian site may be due to abiotic factors such as latitude (13°46'40.53″S), day length, ambient temperatures, or even the precipitation patterns in this mid-tropical montaine site. Epizootic events affecting calliphorids in Brazil strengthen the interest in entomophthoran pathogens for biological control of flies.
[Mh] Termos MeSH primário: Dípteros/microbiologia
Entomophthorales/fisiologia
[Mh] Termos MeSH secundário: Animais
Brasil
Muscidae/microbiologia
Controle Biológico de Vetores
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160313
[St] Status:MEDLINE


  8 / 236 MEDLINE  
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Texto completo SciELO Brasil
[PMID]:26887253
[Au] Autor:Chen C; Xie T; Ye S; Jensen AB; Eilenberg J
[Ad] Endereço:China Jiliang University, Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, Hangzhou 310018, China. Electronic address: aspring@cjlu.edu.cn.
[Ti] Título:Selection of reference genes for expression analysis in the entomophthoralean fungus Pandora neoaphidis.
[So] Source:Braz J Microbiol;47(1):259-65, 2016 Jan-Mar.
[Is] ISSN:1678-4405
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:The selection of suitable reference genes is crucial for accurate quantification of gene expression and can add to our understanding of host-pathogen interactions. To identify suitable reference genes in Pandora neoaphidis, an obligate aphid pathogenic fungus, the expression of three traditional candidate genes including 18S rRNA(18S), 28S rRNA(28S) and elongation factor 1 alpha-like protein (EF1), were measured by quantitative polymerase chain reaction at different developmental stages (conidia, conidia with germ tubes, short hyphae and elongated hyphae), and under different nutritional conditions. We calculated the expression stability of candidate reference genes using four algorithms including geNorm, NormFinder, BestKeeper and Delta Ct. The analysis results revealed that the comprehensive ranking of candidate reference genes from the most stable to the least stable was 18S (1.189), 28S (1.414) and EF1 (3). The 18S was, therefore, the most suitable reference gene for real-time RT-PCR analysis of gene expression under all conditions. These results will support further studies on gene expression in P. neoaphidis.
[Mh] Termos MeSH primário: Entomophthorales/genética
Perfilação da Expressão Gênica/métodos
Perfilação da Expressão Gênica/normas
Genes Fúngicos
Reação em Cadeia da Polimerase em Tempo Real/métodos
Reação em Cadeia da Polimerase em Tempo Real/normas
Padrões de Referência
[Mh] Termos MeSH secundário: Fator 1 de Elongação de Peptídeos/genética
RNA Ribossômico 18S/genética
RNA Ribossômico 28S/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Peptide Elongation Factor 1); 0 (RNA, Ribosomal, 18S); 0 (RNA, Ribosomal, 28S)
[Em] Mês de entrada:1611
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160219
[St] Status:MEDLINE


  9 / 236 MEDLINE  
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[PMID]:26873918
[Au] Autor:Sethi P; Balakrishnan D; Surendran S; Mohamed ZU
[Ad] Endereço:Department of Gastrointestinal Surgery, Amrita Institute of Medical Sciences and Research Centre, Cochin, Kerala, India.
[Ti] Título:Fulminant zygomycosis of graft liver following liver transplantation.
[So] Source:BMJ Case Rep;2016, 2016 Feb 12.
[Is] ISSN:1757-790X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A 44-year-old man with hepatitis B virus (HBV)-related cirrhosis underwent living donor liver transplantation at our institute. Induction of immunosuppression was achieved with basiliximab, due to deranged renal function, and maintained with prednisolone, tacrolimus and mycophenolate mofetil. The intraoperative and immediate postoperative periods were fairly uneventful. A duplex scan, taken during the third week post-transplantation due to sudden rise in liver enzymes, revealed multifocal hypoechoic lesions in the graft liver with normal Doppler parameters. Multidetecor computed tomography (MDCT) showed multiple hypodense vessel-sparing lesions in the graft liver. Cultures from the aspirate grew filamentous fungi identified as Basidiobolus ranarum species. Despite multiple broad spectrum antifungal infusions including liposomal amphotericin, itraconazole, caspofungin and posaconazole, serial sonography showed the hepatic lesions increasing in size, and involving segments V, VI and VII. The patient developed severe liver dysfunction ultimately progressing to sepsis, multiorgan dysfunction and death.
[Mh] Termos MeSH primário: Hepatopatias/etiologia
Transplante de Fígado/efeitos adversos
Zigomicose/etiologia
[Mh] Termos MeSH secundário: Adulto
Antifúngicos/uso terapêutico
Entomophthorales
Evolução Fatal
Seres Humanos
Hepatopatias/tratamento farmacológico
Hepatopatias/microbiologia
Masculino
Zigomicose/tratamento farmacológico
Zigomicose/microbiologia
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160214
[St] Status:MEDLINE


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[PMID]:26825223
[Au] Autor:Kumaravel S; Bharath K; Rajesh NG; Singh R; Kar R
[Ad] Endereço:Departments of 1Pediatric Surgery.
[Ti] Título:Delay and misdiagnosis of basidiobolomycosis in tropical South India: case series and review of the literature.
[So] Source:Paediatr Int Child Health;36(1):52-7, 2016 Feb.
[Is] ISSN:2046-9055
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Basidiobolomycosis caused by Basidiobolus ranarum is a rare infection of the subcutaneous tissues in immune-competent patients which occurs predominantly in children and young adults. Primary physicians often misdiagnose the condition owing to its slow, painless, relentless growth, absence of response to antibiotics and anti-inflammatory drugs and unfamiliarity with this lesion. All cases of subcutaneous basidiobolomycosis in children aged between 1 and 12 years presenting at a tertiary hospital in Pondicherry over a period of 4 years (2007-2011) were analysed. Eight cases were seen and confirmed by histopathology and microbiology. Referral diagnoses varied widely from chronic abscess to tuberculosis to malignancy, including testicular tumour. Lesion sites include the gluteal region, thigh and scrotum (extremely rare). Four weeks of oral itraconazole in seven children and fluconazole (5 mg/kg) in one cured them completely. During follow-up for over 1 year, no recurrence or new lesions were noted. Basidiobolomycosis is a rare, often misdiagnosed infection in children which responds well to triazole antifungals such as itraconazole and fluconazole.
[Mh] Termos MeSH primário: Erros de Diagnóstico
Entomophthorales/isolamento & purificação
Zigomicose/diagnóstico
[Mh] Termos MeSH secundário: Antifúngicos/uso terapêutico
Criança
Pré-Escolar
Diagnóstico Diferencial
Feminino
Fluconazol/uso terapêutico
Seres Humanos
Índia
Lactente
Itraconazol/uso terapêutico
Masculino
Zigomicose/tratamento farmacológico
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Antifungal Agents); 304NUG5GF4 (Itraconazole); 8VZV102JFY (Fluconazole)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170131
[Lr] Data última revisão:
170131
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161021
[St] Status:MEDLINE
[do] DOI:10.1080/20469047.2015.1109220



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