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[PMID]:28792370
[Au] Autor:Zaman K; Rudramurthy SM; Das A; Panda N; Honnavar P; Kaur H; Chakrabarti A
[Ad] Endereço:1​Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Sector 12, Chandigarh 160012, India.
[Ti] Título:Molecular diagnosis of rhino-orbito-cerebral mucormycosis from fresh tissue samples.
[So] Source:J Med Microbiol;66(8):1124-1129, 2017 Aug.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: We aimed to evaluate a PCR-based technique for the diagnosis of mucormycosis and the identification of fungi from fresh tissue specimens in patients with rhino-orbito-cerebral-mucormycosis (ROCM). METHODOLOGY: Fifty cases of ROCM were included in the study. Conventional identification was performed using microscopy and culture. Molecular diagnosis was performed by amplifying the ribosomal DNA using pan-fungal ITS primers and semi-nested Mucorales-specific primers of the 18S region. The amplified products were sequenced to identify the agents. The utility of PCR-RFLP of the 18S region of rDNA was evaluated to identify the Mucorales. RESULTS: The ROCM cases were diagnosed by the demonstration of aseptate ribbon-like hyphae in biopsy specimens collected from the patients. Isolation was possible in 24 (48 %) samples. The ITS2 PCR confirmed mucormycosis in 27 cases (54 %; CI 59.4-68.2). By comparison, Mucorales-specific PCR was able to amplify DNA and the sequence enabled the identification of Mucorales speciesin all the patients. PCR-RFLP of the 18S region of rDNA could only identify the agent to genus level. CONCLUSION: The molecular technique was able to identify Mucorales species in 26 (42 %) cases that were negative by culture. Mucorales-specific semi-nested PCR targeting the 18S region is a better technique than ITS2 PCR for diagnosis. PCR-RFLP of the 18S region helps in identification to genus level.
[Mh] Termos MeSH primário: Mucorales/isolamento & purificação
Mucormicose/diagnóstico
Cavidade Nasal/microbiologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Criança
Pré-Escolar
Primers do DNA/genética
DNA Fúngico/genética
Feminino
Seres Humanos
Masculino
Meia-Idade
Mucorales/genética
Mucormicose/microbiologia
Reação em Cadeia da Polimerase
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Primers); 0 (DNA, Fungal)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170830
[Lr] Data última revisão:
170830
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170810
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000560


  2 / 1076 MEDLINE  
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[PMID]:28599651
[Au] Autor:Kosa G; Kohler A; Tafintseva V; Zimmermann B; Forfang K; Afseth NK; Tzimorotas D; Vuoristo KS; Horn SJ; Mounier J; Shapaval V
[Ad] Endereço:Faculty of Science and Technology, Norwegian University of Life Sciences, Postbox 5003, 1432, Ås, Norway. gergely.kosa@nmbu.no.
[Ti] Título:Microtiter plate cultivation of oleaginous fungi and monitoring of lipogenesis by high-throughput FTIR spectroscopy.
[So] Source:Microb Cell Fact;16(1):101, 2017 Jun 09.
[Is] ISSN:1475-2859
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Oleaginous fungi can accumulate lipids by utilizing a wide range of waste substrates. They are an important source for the industrial production of omega-6 polyunsaturated fatty acids (gamma-linolenic and arachidonic acid) and have been suggested as an alternative route for biodiesel production. Initial research steps for various applications include the screening of fungi in order to find efficient fungal producers with desired fatty acid composition. Traditional cultivation methods (shake flask) and lipid analysis (extraction-gas chromatography) are not applicable for large-scale screening due to their low throughput and time-consuming analysis. Here we present a microcultivation system combined with high-throughput Fourier transform infrared (FTIR) spectroscopy for efficient screening of oleaginous fungi. RESULTS: The microcultivation system enables highly reproducible fungal fermentations throughout 12 days of cultivation. Reproducibility was validated by FTIR and HPLC data. Analysis of FTIR spectral ester carbonyl peaks of fungal biomass offered a reliable high-throughput at-line method to monitor lipid accumulation. Partial least square regression between gas chromatography fatty acid data and corresponding FTIR spectral data was used to set up calibration models for the prediction of saturated fatty acids, monounsaturated fatty acids, polyunsaturated fatty acids, unsaturation index, total lipid content and main individual fatty acids. High coefficients of determination (R = 0.86-0.96) and satisfactory residual predictive deviation of cross-validation (RPD = 2.6-5.1) values demonstrated the goodness of these models. CONCLUSIONS: We have demonstrated in this study, that the presented microcultivation system combined with rapid, high-throughput FTIR spectroscopy is a suitable screening platform for oleaginous fungi. Sample preparation for FTIR measurements can be automated to further increase throughput of the system.
[Mh] Termos MeSH primário: Lipídeos/análise
Lipogênese
Técnicas Microbiológicas
Mucor/metabolismo
Mucorales/metabolismo
Penicillium/metabolismo
Espectroscopia de Infravermelho com Transformada de Fourier
[Mh] Termos MeSH secundário: Biomassa
Reatores Biológicos
Fermentação
Mucor/crescimento & desenvolvimento
Mucorales/crescimento & desenvolvimento
Penicillium/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Lipids)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171108
[Lr] Data última revisão:
171108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170611
[St] Status:MEDLINE
[do] DOI:10.1186/s12934-017-0716-7


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[PMID]:28598997
[Au] Autor:Pathan EK; Ghormade V; Deshpande MV
[Ad] Endereço:Biochemical Sciences Division, AcSIR-CSIR-National Chemical Laboratory, Pune, India.
[Ti] Título:Selection of reference genes for quantitative real-time RT-PCR assays in different morphological forms of dimorphic zygomycetous fungus Benjaminiella poitrasii.
[So] Source:PLoS One;12(6):e0179454, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Benjaminiella poitrasii, a dimorphic non-pathogenic zygomycetous fungus, exhibits a morphological yeast (Y) to hypha (H) reversible transition in the vegetative phase, sporangiospores (S) in the asexual phase and zygospores (Z) in the sexual phase. To study the gene expression across these diverse morphological forms, suitable reference genes are required. In the present study, 13 genes viz. ACT, 18S rRNA, eEF1α, eEF-Tu,eIF-1A, Tub-α, Tub-b, Ubc, GAPDH, Try, WS-21, NADGDH and NADPGDH were evaluated for their potential as a reference, particularly for studying gene expression during the Y-H reversible transition and also for other asexual and sexual life stages of B. poitrasii. Analysis of RT-qPCR data using geNorm, normFinder and BestKeeper software revealed that genes such as Ubc, 18S rRNA and WS-21 were expressed at constant levels in each given subset of RNA samples from all the morphological phases of B. poitrasii. Therefore, these reference genes can be used to elucidate the role of morpho-genes in B. poitrasii. Further, use of the two most stably expressed genes (Ubc and WS-21) to normalize the expression of the ornithine decarboxylase gene (Bpodc) in different morphological forms of B. poitrasii, generated more reliable results, indicating that our selection of reference genes was appropriate.
[Mh] Termos MeSH primário: Genes Fúngicos
Mucorales/classificação
Mucorales/genética
Reação em Cadeia da Polimerase em Tempo Real
[Mh] Termos MeSH secundário: AMP Cíclico/metabolismo
Perfilação da Expressão Gênica
Regulação Fúngica da Expressão Gênica
Estágios do Ciclo de Vida
Mucorales/citologia
Mucorales/crescimento & desenvolvimento
NADP/metabolismo
Esporos Fúngicos
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
53-59-8 (NADP); E0399OZS9N (Cyclic AMP)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170610
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179454


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[PMID]:28355462
[Au] Autor:Lass-Flörl C; Cuenca-Estrella M
[Ad] Endereço:Division of Hygiene and Medical Microbiology, Medical University of Innsbruck, Schöpfstraße 41, 6020 Innsbruck, Austria.
[Ti] Título:Changes in the epidemiological landscape of invasive mould infections and disease.
[So] Source:J Antimicrob Chemother;72(suppl_1):i5-i11, 2017 Mar 01.
[Is] ISSN:1460-2091
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Although a wide variety of pathogens are associated with invasive mould diseases, Aspergillus spp. have historically been one of the most common causative organisms. Most invasive mould infections are caused by members of the Aspergillus fumigatus species complex and an emerging issue is the occurrence of azole resistance in A. fumigatus, with resistance to amphotericin B documented in other Aspergillus spp. The epidemiology of invasive fungal disease has shifted in recent years as non-A. fumigatus Aspergillus spp. and other moulds have become progressively more important, although there are no consolidated data on the prevalence of less common species of moulds. The incidence of mucormycosis may have been underestimated, which is a potential concern since species belonging to the order Mucorales are more resistant to antifungal agents than Aspergillus spp. All species of Mucorales are unaffected by voriconazole and most show moderate resistance in vitro to echinocandins. Fusarium spp. may be the second most common nosocomial fungal pathogen after Aspergillus in some tertiary hospitals, and show a susceptibility profile marked by a higher level of resistance than that of Aspergillus spp. Recently, Scedosporium aurantiacum has been reported as an emerging opportunistic pathogen, against which voriconazole is the most active antifungal agent. Other mould species can infect humans, although invasive fungal disease occurs less frequently. Since uncommon mould species exhibit individual susceptibility profiles and require tailored clinical management, accurate classification at species level of the aetiological agent in any invasive fungal disease should be regarded as the standard of care.
[Mh] Termos MeSH primário: Antifúngicos/uso terapêutico
Aspergilose/tratamento farmacológico
Aspergilose/epidemiologia
Aspergillus fumigatus/efeitos dos fármacos
Farmacorresistência Fúngica/fisiologia
Infecções Fúngicas Invasivas/tratamento farmacológico
[Mh] Termos MeSH secundário: Aspergilose/microbiologia
Fusariose/tratamento farmacológico
Fusariose/epidemiologia
Fusariose/microbiologia
Fusarium/efeitos dos fármacos
Seres Humanos
Hospedeiro Imunocomprometido
Infecções Fúngicas Invasivas/imunologia
Infecções Fúngicas Invasivas/microbiologia
Mucorales/efeitos dos fármacos
Mucormicose/tratamento farmacológico
Mucormicose/epidemiologia
Mucormicose/microbiologia
Scedosporium/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170330
[St] Status:MEDLINE
[do] DOI:10.1093/jac/dkx028


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[PMID]:28332467
[Au] Autor:Lee SC; Idnurm A
[Ad] Endereço:South Texas Center for Emerging Infectious Diseases (STCEID), Department of Biology, University of Texas at San Antonio, San Antonio, TX 78249.
[Ti] Título:Fungal Sex: The Mucoromycota.
[So] Source:Microbiol Spectr;5(2), 2017 Mar.
[Is] ISSN:2165-0497
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Although at the level of resolution of genes and molecules most information about mating in fungi is from a single lineage, the Dikarya, many fundamental discoveries about mating in fungi have been made in the earlier branches of the fungi. These are nonmonophyletic groups that were once classified into the chytrids and zygomycetes. Few species in these lineages offer the potential of genetic tractability, thereby hampering the ability to identify the genes that underlie those fundamental insights. Research performed during the past decade has now established the genes required for mating type determination and pheromone synthesis in some species in the phylum Mucoromycota, especially in the order Mucorales. These findings provide striking parallels with the evolution of mating systems in the Dikarya fungi. Other discoveries in the Mucorales provide the first examples of sex-cell type identity being driven directly by a gene that confers mating type, a trait considered more of relevance to animal sex determination but difficult to investigate in animals. Despite these discoveries, there remains much to be gleaned about mating systems from these fungi.
[Mh] Termos MeSH primário: Genes Fúngicos Tipo Acasalamento
Recombinação Homóloga
Mucorales/crescimento & desenvolvimento
Mucorales/genética
[Mh] Termos MeSH secundário: Feromônios/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Pheromones)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170705
[Lr] Data última revisão:
170705
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170324
[St] Status:MEDLINE
[do] DOI:10.1128/microbiolspec.FUNK-0041-2017


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[PMID]:28106750
[Au] Autor:Winska K; Grabarczyk M; Maczka W; Kondas A; Maciejewska G; Bonikowski R; Aniol M
[Ad] Endereço:Department of Chemistry, Wroclaw University of Environmental and Life Sciences, Norwida 25, 50-375 Wroclaw, Poland. katarzyna.winska@upwr.edu.pl.
[Ti] Título:Synthesis and Biotransformation of Bicyclic Unsaturated Lactones with Three or Four Methyl Groups.
[So] Source:Molecules;22(1), 2017 Jan 17.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to obtain new unsaturated lactones by chemical synthesis and their microbial transformations using fungal strains. Some of these strains were able to transform unsaturated lactones into different hydroxy or epoxy derivatives. Strains of and gave products with a hydroxy group introduced into a tertiary carbon, while the strain hydroxylated primary carbons. The strain hydroxylated both substrates in an allylic position. Using the and strains led to the obtained epoxylactones. The structures of all lactones were established on the basis of spectroscopic data.
[Mh] Termos MeSH primário: Biotransformação
Lactonas/síntese química
Lactonas/metabolismo
[Mh] Termos MeSH secundário: Absidia/metabolismo
Hidroxilação
Mucorales/metabolismo
Penicillium/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Lactones)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170512
[Lr] Data última revisão:
170512
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170121
[St] Status:MEDLINE


  7 / 1076 MEDLINE  
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[PMID]:27937112
[Au] Autor:Ibrahim M; Kaushik N; Sowemimo A; Chhipa H; Koekemoer T; van de Venter M; Odukoya OA
[Ad] Endereço:a Department of Pharmacognosy, Faculty of Pharmacy , University of Lagos, College of Medicine campus , Lagos , Nigeria.
[Ti] Título:Antifungal and antiproliferative activities of endophytic fungi isolated from the leaves of Markhamia tomentosa.
[So] Source:Pharm Biol;55(1):590-595, 2017 Dec.
[Is] ISSN:1744-5116
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:CONTEXT: Plants harbor endophytes with potential bioactivity. Markhamia tomentosa (Benth) K. Schum ex. Engl. (Bignoniaceae) is reported to possess antioxidant, anti-inflammatory and anticancer activities. OBJECTIVE: The antifungal and antiproliferative properties of endophytic fungi extracts and fractions from M. tomentosa were evaluated. MATERIAL AND METHODS: Endophytic fungi were isolated from the leaves of M. tomentosa and identified by ITS-rDNA sequence analysis. The antagonistic effect of the fungal strains was investigated against pathogenic fungi viz, Fusarium oxysporum, Sclerotinia sclerotiorium, Rhizoctonia solani, and Botrytis cinerea using the dual culture assay for 5-7 days. Antiproliferative effect of the fungal extracts and fractions (3.91-250 µg/mL) on HeLa cancer cell line was tested and IC was calculated. Poisoning food assay and antifeedant activity against the pathogenic fungi and Spodoptera litura larvae, for 7 days and 2 h, respectively, was also tested at concentrations of 250, 500 and 1000 µg/mL. RESULTS: Fungal endophytes Trichoderma longibrachiatum and Syncephalastrum racemosum were isolated from the leaves of M. tomentosa. Isolated endophytic fungal strains and solvent extracts showed MIC value of 1000 µg/mL against tested pathogenic fungi in the dual culture and poisoning food assays. Methanol fraction of S. racemosum isolate showed the most effective antiproliferative activity with IC of 43.56 µg/mL. Minimal feeding deterrent activity against S. litura larvae was also observed. DISCUSSION AND CONCLUSION: These findings showed that the leaves of Markhamia tomentosa harbor strains of endophytic fungi with promising health benefits, and suggest their antifungal and antiproliferative effects against pathogenic fungi and HeLa cancer cell line.
[Mh] Termos MeSH primário: Antifúngicos/farmacologia
Antineoplásicos Fitogênicos/farmacologia
Bignoniaceae/microbiologia
Proliferação Celular/efeitos dos fármacos
Endófitos/metabolismo
Mucorales/metabolismo
Extratos Vegetais/farmacologia
Folhas de Planta/microbiologia
Trichoderma/metabolismo
Neoplasias do Colo do Útero/tratamento farmacológico
[Mh] Termos MeSH secundário: Animais
Antifúngicos/isolamento & purificação
Antineoplásicos Fitogênicos/isolamento & purificação
Ascomicetos/efeitos dos fármacos
Ascomicetos/crescimento & desenvolvimento
Bignoniaceae/química
Botrytis/efeitos dos fármacos
Botrytis/crescimento & desenvolvimento
Relação Dose-Resposta a Droga
Endófitos/isolamento & purificação
Feminino
Fermentação
Fusarium/efeitos dos fármacos
Fusarium/crescimento & desenvolvimento
Células HeLa
Seres Humanos
Concentração Inibidora 50
Larva/efeitos dos fármacos
Testes de Sensibilidade Microbiana
Mucorales/isolamento & purificação
Fitoterapia
Extratos Vegetais/isolamento & purificação
Folhas de Planta/química
Plantas Medicinais
Rhizoctonia/efeitos dos fármacos
Rhizoctonia/crescimento & desenvolvimento
Spodoptera/efeitos dos fármacos
Spodoptera/crescimento & desenvolvimento
Fatores de Tempo
Trichoderma/isolamento & purificação
Neoplasias do Colo do Útero/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Antineoplastic Agents, Phytogenic); 0 (Plant Extracts)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170313
[Lr] Data última revisão:
170313
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161213
[St] Status:MEDLINE


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[PMID]:27876506
[Au] Autor:Bellanger AP; Lignon T; Godet Y; Rognon B; Reboux G; Gbaguidi-Haore H; Borg C; Millon L
[Ad] Endereço:Chrono-Environnement CNRS 6249 Research Team, Franche-Comté University, Besançon, France; Parasitology-Mycology Department, Besançon University Hospital, Besançon, France. Electronic address: apbellanger@chu-besancon.fr.
[Ti] Título:Fungal peptides from pneumonitis hypersensitivity etiologic agents are able to induce specific cellular immune response.
[So] Source:J Immunol Methods;440:67-73, 2017 Jan.
[Is] ISSN:1872-7905
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:PURPOSE: Hypersensitivity pneumonitis (HP) is an immunoallergic disease due to chronic exposure to high quantities of different microorganisms such as Mycobacterium immunogenum (Mi), a mycobacterium, and Lichtheimia corymbifera (Lc), a filamentous fungus. It has recently been demonstrated that the protein DLDH (dihydrolipoyl dehydrogenase), is common to these microorganisms. This study aimed to investigate the immune potential of overlapping peptide pools covering the MiDLDH and LcDLDH. EXPERIMENTAL DESIGN: A selection of 34 peptides, from the MiDLDH and LcDLDH, able to interact with Major Histocompatibility Complex (MHC) 1 and MHC 2, was obtained using three different epitope prediction websites. By means of ELISPOT assays, we compared the frequency of Interferon gamma (IFNγ) secreting peripheral blood mononuclear cells (PBMC) after stimulation with overlapping peptide pools. Tests were performed using cells from 35 healthy blood donors. RESULTS: One peptide pool containing five peptides from MiDLDH and able to interact with MHC 2 induced a marked IFNγ specific immune response (Pool F, p<0.001, Wilcoxon signed-rank test). CONCLUSION: This study demonstrated that peptides from microorganisms involved in HP were able to induce a high IFNγ specific immune response after stimulation of PBMCs from healthy blood donors which could be useful to develop an effective prevention strategy.
[Mh] Termos MeSH primário: Alveolite Alérgica Extrínseca/imunologia
Antígenos de Bactérias/imunologia
Antígenos de Fungos/imunologia
Di-Hidrolipoamida Desidrogenase/imunologia
Imunidade Celular
Mucorales/imunologia
Mycobacterium/imunologia
Fragmentos de Peptídeos/imunologia
[Mh] Termos MeSH secundário: Adulto
Idoso
Alveolite Alérgica Extrínseca/sangue
Alveolite Alérgica Extrínseca/microbiologia
Células Cultivadas
ELISPOT
Mapeamento de Epitopos
Epitopos
Feminino
Voluntários Saudáveis
Antígenos de Histocompatibilidade Classe I/imunologia
Antígenos de Histocompatibilidade Classe II/imunologia
Seres Humanos
Interferon gama/imunologia
Interferon gama/metabolismo
Leucócitos Mononucleares/imunologia
Leucócitos Mononucleares/microbiologia
Masculino
Meia-Idade
Mucorales/enzimologia
Mycobacterium/enzimologia
Estatísticas não Paramétricas
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Bacterial); 0 (Antigens, Fungal); 0 (Epitopes); 0 (Histocompatibility Antigens Class I); 0 (Histocompatibility Antigens Class II); 0 (IFNG protein, human); 0 (Peptide Fragments); 82115-62-6 (Interferon-gamma); EC 1.8.1.4 (Dihydrolipoamide Dehydrogenase)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161124
[St] Status:MEDLINE


  9 / 1076 MEDLINE  
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[PMID]:27869536
[Au] Autor:Chung H; Lee N; Seo JA; Kim YS
[Ad] Endereço:a Department of Food Science and Engineering , Ewha Womans University , Seoul , Korea.
[Ti] Título:Comparative analysis of nonvolatile and volatile metabolites in Lichtheimia ramosa cultivated in different growth media.
[So] Source:Biosci Biotechnol Biochem;81(3):565-572, 2017 Mar.
[Is] ISSN:1347-6947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Lichtheimia ramosa is one of the predominant filamentous fungi in Korean traditional nuruk. The nonvolatile and volatile metabolites of L. ramosa cultivated in three growth media: complete medium (CM), potato dextrose broth (PDB), and sabouraud dextrose broth (SDB), were investigated and compared. Among nonvolatile metabolites, serine, lysine, and ornithine increased in CM and PDB cultivated with L. ramosa during the exponential phase. In addition, glucose level increased in CM whereas decreased in PDB and SDB. The major volatile metabolites in the extract samples were acetic acid, ethanol, 3-methyl-2-buten-1-ol, 2-phenylethanol, ethylacetate, 2-furaldehyde, 5-(hydroxymethyl)-2-furaldehyde, 2,3-dihydro-3,5,-dihydroxy-6-methyl-4H-pyran-4-one, and α-humulene. In particular, the levels of volatile metabolites related to makgeolli (e.g., acetic acid, ethanol, and ethyl acetate) were highest in extracts cultivated in CM. On the other hand, the level of 2-phenylethanol was relatively higher in PDB and SDB, possibly due to there being more phenylalanine present in the biomass sample in media.
[Mh] Termos MeSH primário: Meios de Cultura/química
Microbiologia de Alimentos
Mucorales/metabolismo
Compostos Orgânicos Voláteis/análise
[Mh] Termos MeSH secundário: Aminoácidos/análise
Aminoácidos/metabolismo
Meios de Cultura/metabolismo
Ciclopentanos/metabolismo
Ácidos Graxos/análise
Ácidos Graxos/metabolismo
Microbiologia de Alimentos/métodos
Cromatografia Gasosa-Espectrometria de Massas/métodos
Glucose/metabolismo
Mucorales/citologia
Mucorales/crescimento & desenvolvimento
Álcool Feniletílico/metabolismo
Sesquiterpenos/metabolismo
Compostos Orgânicos Voláteis/metabolismo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Culture Media); 0 (Cyclopentanes); 0 (Fatty Acids); 0 (Sesquiterpenes); 0 (Volatile Organic Compounds); 54W56MD2WD (humulene); IY9XDZ35W2 (Glucose); ML9LGA7468 (Phenylethyl Alcohol)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170306
[Lr] Data última revisão:
170306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161122
[St] Status:MEDLINE
[do] DOI:10.1080/09168451.2016.1256756


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[PMID]:27830590
[Au] Autor:Wang L; Qin D; Zhang K; Huang Q; Liu S; Han MJ; Dong JY
[Ad] Endereço:a Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences , Southwest University , Chongqing , People's Republic of China.
[Ti] Título:Metabolites from the co-culture of nigranoic acid and Umbelopsis dimorpha SWUKD3.1410, an endophytic fungus from Kadsura angustifolia.
[So] Source:Nat Prod Res;31(12):1414-1421, 2017 Jun.
[Is] ISSN:1478-6427
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The ability of the endophytic fungus Umbelopsis dimorpha SWUKD3.1410 to transform the triterpene nigranoic acid (3, 4-secocycloarta-4(28), 24-(Z)-diene-3, 26-dioic acid; 1) was investigated. Co-culture of nigranoic acid with U. dimorpha SWUKD3.1410 yielded six major products, including a new triterpene (2) and 5 known compounds (3-7). Their structures were identified by the extensive 1D, 2D NMR and HR-ESI-MS Data analysis, and by comparison with those reported data. Among them, 2-4 were transformed from nigranoic acid (1), while 5-7 were derived from the culture medium stimulated by the substrate. On the basis of the structures of 2-4, it was proposed that the transformative process probably involved isomerase and hydroxylase, in which the former was rare in fungi. Additionally, all the compounds (1-7) were evaluated for their cytotoxic activity. As a result, compounds 1, 3-5 exhibited weak cytotoxicity against the tested cell lines, while others showed no cytotoxicity.
[Mh] Termos MeSH primário: Endófitos/metabolismo
Kadsura/microbiologia
Mucorales/metabolismo
Triterpenos/metabolismo
[Mh] Termos MeSH secundário: Antineoplásicos/farmacologia
Linhagem Celular Tumoral
Técnicas de Cocultura
Seres Humanos
Espectroscopia de Ressonância Magnética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Triterpenes); 0 (nigranoic acid)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170609
[Lr] Data última revisão:
170609
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161111
[St] Status:MEDLINE
[do] DOI:10.1080/14786419.2016.1255891



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