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[PMID]:29362365
[Au] Autor:Zhang Y; Kastman EK; Guasto JS; Wolfe BE
[Ad] Endereço:Department of Biology, Tufts University, 200 Boston Avenue, Medford, MA, 02155, USA.
[Ti] Título:Fungal networks shape dynamics of bacterial dispersal and community assembly in cheese rind microbiomes.
[So] Source:Nat Commun;9(1):336, 2018 01 23.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Most studies of bacterial motility have examined small-scale (micrometer-centimeter) cell dispersal in monocultures. However, bacteria live in multispecies communities, where interactions with other microbes may inhibit or facilitate dispersal. Here, we demonstrate that motile bacteria in cheese rind microbiomes use physical networks created by filamentous fungi for dispersal, and that these interactions can shape microbial community structure. Serratia proteamaculans and other motile cheese rind bacteria disperse on fungal networks by swimming in the liquid layers formed on fungal hyphae. RNA-sequencing, transposon mutagenesis, and comparative genomics identify potential genetic mechanisms, including flagella-mediated motility, that control bacterial dispersal on hyphae. By manipulating fungal networks in experimental communities, we demonstrate that fungal-mediated bacterial dispersal can shift cheese rind microbiome composition by promoting the growth of motile over non-motile community members. Our single-cell to whole-community systems approach highlights the interactive dynamics of bacterial motility in multispecies microbiomes.
[Mh] Termos MeSH primário: Queijo/microbiologia
DNA Bacteriano/genética
Fungos/crescimento & desenvolvimento
Hifas/crescimento & desenvolvimento
Interações Microbianas/genética
Microbiota/genética
Serratia/genética
[Mh] Termos MeSH secundário: Actinobacteria/classificação
Actinobacteria/genética
Actinobacteria/crescimento & desenvolvimento
Elementos de DNA Transponíveis
Firmicutes/classificação
Firmicutes/genética
Firmicutes/crescimento & desenvolvimento
Flagelos/genética
Flagelos/ultraestrutura
Fungos/ultraestrutura
Sequenciamento de Nucleotídeos em Larga Escala
Hifas/ultraestrutura
Movimento/fisiologia
Mucor/crescimento & desenvolvimento
Mucor/ultraestrutura
Mutação
Penicillium/crescimento & desenvolvimento
Penicillium/ultraestrutura
Proteobactérias/classificação
Proteobactérias/genética
Proteobactérias/crescimento & desenvolvimento
Serratia/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (DNA Transposable Elements); 0 (DNA, Bacterial)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180125
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02522-z


  2 / 1455 MEDLINE  
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[PMID]:29260551
[Au] Autor:Zhang L; Zhang H; Song Y
[Ad] Endereço:Colin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology , Zibo, 255049 Shandong, People's Republic of China.
[Ti] Título:Identification and Characterization of Diacylglycerol Acyltransferase from Oleaginous Fungus Mucor circinelloides.
[So] Source:J Agric Food Chem;66(3):674-681, 2018 Jan 24.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Acyl-CoA:diacylglycerol acyltransferase (DGAT) is a pivotal regulator of triacylglycerol (TAG) synthesis. The oleaginous fungus Mucor circinelloides has four putative DGATs: McDGAT1A, McDGAT1B, McDGAT2A, and McDGAT2B, classified into the DGAT1 and DGAT2 subfamilies, respectively. To identify and characterize DGATs in M. circinelloides, these four genes were expressed in Saccharomyces cerevisiae H1246 (TAG-deficient quadruple mutant), individually. TAG biosynthesis was restored only by the expression of McDGAT2B, and TAG content was significantly higher in the mutants with McDGAT2B expression than in a S. cerevisiae mutant with endogenous DGA1 expression. McDGAT2B prefers saturated fatty acids to monounsaturated fatty acids and has an obvious preference for C18:3 (ω-6) according to the results of substrate preference experiments. Furthermore, only the mRNA expression pattern of McDGAT2B correlated with TAG biosynthesis during a fermentation process. Our experiments strongly indicate that McDGAT2B is crucial for TAG accumulation, suggesting that it may be an essential target for metabolic engineering aimed at increasing lipid content of M. circinelloides.
[Mh] Termos MeSH primário: Diacilglicerol O-Aciltransferase/química
Proteínas Fúngicas/química
Mucor/enzimologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Diacilglicerol O-Aciltransferase/genética
Diacilglicerol O-Aciltransferase/metabolismo
Ácidos Graxos/química
Ácidos Graxos/metabolismo
Proteínas Fúngicas/genética
Proteínas Fúngicas/metabolismo
Dados de Sequência Molecular
Mucor/química
Mucor/genética
Família Multigênica
Alinhamento de Sequência
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fatty Acids); 0 (Fungal Proteins); EC 2.3.1.20 (Diacylglycerol O-Acyltransferase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180207
[Lr] Data última revisão:
180207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171221
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b04295


  3 / 1455 MEDLINE  
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[PMID]:28859156
[Au] Autor:Barman S; Ghosh R; Sengupta S; Mandal NC
[Ad] Endereço:Mycology and Plant Pathology Laboratory, Department of Botany, Visva-Bharati, Santiniketan, West Bengal, India.
[Ti] Título:Longterm storage of post-packaged bread by controlling spoilage pathogens using Lactobacillus fermentum C14 isolated from homemade curd.
[So] Source:PLoS One;12(8):e0184020, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:One potent lactic acid bacterial strain C14 with strong antifungal activity was isolated from homemade curd. Based on morphological as well as biochemical characters and 16S rDNA sequence homology the strain was identified as Lactobacillus fermentum. It displayed a wide antimicrobial spectrum against both Gram-positive and Gram-negative pathogenic bacteria, and also against number of food spoilage, plant and human pathogenic fungi. The cell free supernatant (CFS) of the strain C14 was also effective against the fungi tested. Inhibition of radial growth of Penicillium digitatum, Trichophyton rubrum and Mucor sp. was noticed in the presence of CFS of C14 even at low concentration (1%). More than 94.3 ± 1.6% and 91.5 ± 2.2% inhibition of conidial germination of P. digitatum and Mucor sp. were noticed in the presence of 10-fold-concentrated CFS of C14. Massive deformation of the fungal mycelia was observed by SEM studies, and losses of cellular proteins and DNA are also evident upon its treatment with C14. HPLC analysis revealed the presence of phenyl lactic acid, lactic acid along with some unidentified compounds in the antifungal extract. Challenge experiment showed immense potential of the strain C14 in preventing the spoilage of bread samples caused by Mucor sp. and Bacillus subtilis. The bread samples remained fresh upto 25 days even after inoculation with Mucor sp. (3.7 × 104 spores /ml) and B. subtilis (4.6 × 104 CFU /ml). Along with the antifungal properties, the isolated lactic acid bacterial strain also showed very good antioxidant activities. Unchanged level of liver enzymes serum glutamic pyruvic transaminase and serum glutamic oxaloacetic transaminase in albino mice upon feeding with C14 also suggested non-toxic nature of the bacterial isolate.
[Mh] Termos MeSH primário: Anti-Infecciosos/farmacologia
Antibiose
Conservantes de Alimentos/farmacologia
Lactatos/farmacologia
Ácido Láctico/farmacologia
Lactobacillus fermentum/química
Leite/microbiologia
[Mh] Termos MeSH secundário: Alanina Transaminase/metabolismo
Animais
Anti-Infecciosos/isolamento & purificação
Aspartato Aminotransferases/sangue
Pão
Fermentação
Conservantes de Alimentos/isolamento & purificação
Armazenamento de Alimentos/métodos
Bactérias Gram-Negativas/efeitos dos fármacos
Bactérias Gram-Negativas/crescimento & desenvolvimento
Bactérias Gram-Positivas/efeitos dos fármacos
Bactérias Gram-Positivas/crescimento & desenvolvimento
Seres Humanos
Lactatos/isolamento & purificação
Ácido Láctico/isolamento & purificação
Lactobacillus fermentum/isolamento & purificação
Masculino
Camundongos
Mucor/efeitos dos fármacos
Mucor/crescimento & desenvolvimento
Micélio/efeitos dos fármacos
Micélio/crescimento & desenvolvimento
Penicillium/efeitos dos fármacos
Penicillium/crescimento & desenvolvimento
Trichophyton/efeitos dos fármacos
Trichophyton/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Food Preservatives); 0 (Lactates); 156-05-8 (3-phenyllactic acid); 33X04XA5AT (Lactic Acid); EC 2.6.1.1 (Aspartate Aminotransferases); EC 2.6.1.2 (Alanine Transaminase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170901
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184020


  4 / 1455 MEDLINE  
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[PMID]:28599651
[Au] Autor:Kosa G; Kohler A; Tafintseva V; Zimmermann B; Forfang K; Afseth NK; Tzimorotas D; Vuoristo KS; Horn SJ; Mounier J; Shapaval V
[Ad] Endereço:Faculty of Science and Technology, Norwegian University of Life Sciences, Postbox 5003, 1432, Ås, Norway. gergely.kosa@nmbu.no.
[Ti] Título:Microtiter plate cultivation of oleaginous fungi and monitoring of lipogenesis by high-throughput FTIR spectroscopy.
[So] Source:Microb Cell Fact;16(1):101, 2017 Jun 09.
[Is] ISSN:1475-2859
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Oleaginous fungi can accumulate lipids by utilizing a wide range of waste substrates. They are an important source for the industrial production of omega-6 polyunsaturated fatty acids (gamma-linolenic and arachidonic acid) and have been suggested as an alternative route for biodiesel production. Initial research steps for various applications include the screening of fungi in order to find efficient fungal producers with desired fatty acid composition. Traditional cultivation methods (shake flask) and lipid analysis (extraction-gas chromatography) are not applicable for large-scale screening due to their low throughput and time-consuming analysis. Here we present a microcultivation system combined with high-throughput Fourier transform infrared (FTIR) spectroscopy for efficient screening of oleaginous fungi. RESULTS: The microcultivation system enables highly reproducible fungal fermentations throughout 12 days of cultivation. Reproducibility was validated by FTIR and HPLC data. Analysis of FTIR spectral ester carbonyl peaks of fungal biomass offered a reliable high-throughput at-line method to monitor lipid accumulation. Partial least square regression between gas chromatography fatty acid data and corresponding FTIR spectral data was used to set up calibration models for the prediction of saturated fatty acids, monounsaturated fatty acids, polyunsaturated fatty acids, unsaturation index, total lipid content and main individual fatty acids. High coefficients of determination (R = 0.86-0.96) and satisfactory residual predictive deviation of cross-validation (RPD = 2.6-5.1) values demonstrated the goodness of these models. CONCLUSIONS: We have demonstrated in this study, that the presented microcultivation system combined with rapid, high-throughput FTIR spectroscopy is a suitable screening platform for oleaginous fungi. Sample preparation for FTIR measurements can be automated to further increase throughput of the system.
[Mh] Termos MeSH primário: Lipídeos/análise
Lipogênese
Técnicas Microbiológicas
Mucor/metabolismo
Mucorales/metabolismo
Penicillium/metabolismo
Espectroscopia de Infravermelho com Transformada de Fourier
[Mh] Termos MeSH secundário: Biomassa
Reatores Biológicos
Fermentação
Mucor/crescimento & desenvolvimento
Mucorales/crescimento & desenvolvimento
Penicillium/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Lipids)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171108
[Lr] Data última revisão:
171108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170611
[St] Status:MEDLINE
[do] DOI:10.1186/s12934-017-0716-7


  5 / 1455 MEDLINE  
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[PMID]:28557429
[Au] Autor:Tang X; Chen H; Gu Z; Zhang H; Chen YQ; Song Y; Chen W
[Ad] Endereço:State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University , Wuxi 214122, P.R. China.
[Ti] Título:Comparative Proteome Analysis between High Lipid-Producing Strain Mucor circinelloides WJ11 and Low Lipid-Producing Strain CBS 277.49.
[So] Source:J Agric Food Chem;65(24):5074-5082, 2017 Jun 21.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mucor circinelloides is one of few oleaginous fungi that produces a useful oil rich in γ-linolenic acid, but it usually only produces <25% total lipid. Nevertheless, we isolated a new strain WJ11 that can produce up to 36% lipid of cell dry weight. In this study, we have systematically analyzed the global changes in protein levels between the high lipid-producing strain WJ11 and the low lipid-producing strain CBS 277.49 (15%, lipid/cell dry weight) at lipid accumulation phase through comparative proteome analysis. Proteome analysis demonstrated that the branched-chain amino acid and lysine metabolism, glycolytic pathway, and pentose phosphate pathway in WJ11 were up-regulated, while the activities of tricarboxylic acid cycle and branch point enzyme for synthesis of isoprenoids were retarded compared with CBS 277.49. The coordinated regulation at proteome level indicate that more acetyl-CoA and NADPH are provided for fatty acid biosynthesis in WJ11 compared with CBS 277.49.
[Mh] Termos MeSH primário: Proteínas Fúngicas/metabolismo
Lipídeos/biossíntese
Mucor/metabolismo
Proteoma/metabolismo
[Mh] Termos MeSH secundário: Aminoácidos de Cadeia Ramificada/análise
Aminoácidos de Cadeia Ramificada/metabolismo
Proteínas Fúngicas/química
Proteínas Fúngicas/genética
Mucor/química
Mucor/classificação
Mucor/genética
Proteoma/química
Proteoma/genética
Ácido gama-Linolênico/análise
Ácido gama-Linolênico/metabolismo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids, Branched-Chain); 0 (Fungal Proteins); 0 (Lipids); 0 (Proteome); 78YC2MAX4O (gamma-Linolenic Acid)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170703
[Lr] Data última revisão:
170703
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170531
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b00935


  6 / 1455 MEDLINE  
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[PMID]:28538103
[Au] Autor:Crutcher FK; Puckhaber LS; Bell AA; Liu J; Duke SE; Stipanovic RD; Nichols RL
[Ad] Endereço:USDA, Insect Control and Cotton Disease Research Unit, Southern Plains Agricultural Research Center, Agricultural Research Service, USDA , 2765 F&B Road, College Station, Texas 77845, United States.
[Ti] Título:Detoxification of Fusaric Acid by the Soil Microbe Mucor rouxii.
[So] Source:J Agric Food Chem;65(24):4989-4992, 2017 Jun 21.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Fusarium oxysporum f. sp. vasinfectum race 4 (VCG0114), which causes root rot and wilt of cotton (Gossypium hirsutum and G. barbadense), has been identified recently for the first time in the western hemisphere in certain fields in the San Joaquin Valley of California. This pathotype produces copious quantities of the plant toxin fusaric acid (5-butyl-2-pyridinecarboxylic acid) compared to other isolates of F. oxysporum f. sp. vasinfectum (Fov) that are indigenous to the United States. Fusaric acid is toxic to cotton plants and may help the pathogen compete with other microbes in the soil. We found that a laboratory strain of the fungus Mucor rouxii converts fusaric acid into a newly identified compound, 8-hydroxyfusaric acid. The latter compound is significantly less phytotoxic to cotton than the parent compound. On the basis of bioassays of hydroxylated analogues of fusaric acid, hydroxylation of the butyl side chain of fusaric acid may affect a general detoxification of fusaric acid. Genes that control this hydroxylation may be useful in developing biocontrol agents to manage Fov.
[Mh] Termos MeSH primário: Ácido Fusárico/metabolismo
Fusarium/fisiologia
Gossypium/microbiologia
Mucor/metabolismo
Doenças das Plantas/microbiologia
Toxinas Biológicas/metabolismo
[Mh] Termos MeSH secundário: Biotransformação
Proteínas Fúngicas/química
Proteínas Fúngicas/genética
Proteínas Fúngicas/metabolismo
Ácido Fusárico/química
Ácido Fusárico/toxicidade
Estrutura Molecular
Mucor/genética
Microbiologia do Solo
Toxinas Biológicas/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungal Proteins); 0 (Toxins, Biological); JWJ963070N (Fusaric Acid)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170703
[Lr] Data última revisão:
170703
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170525
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b01655


  7 / 1455 MEDLINE  
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[PMID]:28502711
[Au] Autor:Hernández-García S; García-García MI; García-Carmona F
[Ad] Endereço:Department of Biochemistry and Molecular Biology-A, Faculty of Biology, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, Campus Espinardo, E-30100 Murcia, Spain. Electronic address: samanta.hernandez@um.es.
[Ti] Título:An improved method to measure lipase activity in aqueous media.
[So] Source:Anal Biochem;530:104-106, 2017 Aug 01.
[Is] ISSN:1096-0309
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:An improved method based on the p-nitrophenyl long chain esters method is proposed for measuring lipase hydrolytic activity in aqueous media. Using ethylene glycol as co-solvent for hydrophobic p-nitrophenyl substrates in aqueous buffer, lipase activity is measured by following the release of p-nitrophenol. This fast and easy to handle method improves the solubility of both substrate and product, and also the stability of the substrate. It avoids the use of solvents such as ethanol or propanol, permits the comparison of all the p-nitrophenol acyl ester substrates and allows the influence of ions like Ca to be studied, while avoiding turbidity in the reaction medium.
[Mh] Termos MeSH primário: Lipase/metabolismo
Mucor/enzimologia
Nitrofenóis/metabolismo
Solventes/química
Água/química
[Mh] Termos MeSH secundário: Concentração de Íons de Hidrogênio
Interações Hidrofóbicas e Hidrofílicas
Cinética
Solubilidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Nitrophenols); 0 (Solvents); 059QF0KO0R (Water); EC 3.1.1.3 (Lipase); Y92ZL45L4R (4-nitrophenol)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170516
[St] Status:MEDLINE


  8 / 1455 MEDLINE  
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[PMID]:28501431
[Au] Autor:Knappmann I; Lehmkuhl K; Köhler J; Schepmann D; Giera M; Bracher F; Wünsch B
[Ad] Endereço:Institut für Pharmazeutische und Medizinische Chemie der Universität Münster, Corrensstraße 48, D-48149 Münster, Germany.
[Ti] Título:Lipase-catalyzed kinetic resolution as key step in the synthesis of enantiomerically pure σ ligands with 2-benzopyran structure.
[So] Source:Bioorg Med Chem;25(13):3384-3395, 2017 Jul 01.
[Is] ISSN:1464-3391
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In order to obtain enantiomerically pure σ receptor ligands with a 2-benzopyran scaffold an Oxa-Pictet-Spengler reaction with the enantiomerically pure 2-phenylethanol derivatives (R)-4 and (S)-4 was envisaged. The kinetic resolution of racemic alcohol (±)-4 using Amano Lipase PS-C II and isopropenyl acetate in tert-butyl methyl ether led to the (R)-configured alcohol (R)-4 in 42% yield with an enantiomeric excess of 99.6%. The (S)-configured alcohol (S)-4 was obtained by Amano Lipase PS-C II catalyzed hydrolysis of enantiomerically enriched acetate (S)-5 (76.9% ee) and provided (S)-4 in 26% yield and 99.7% ee. The absolute configuration of alcohol (R)-4 was determined by exciton coupled CD spectroscopy of the bis(bromobenzoate) (R)-7. The next important step for the synthesis of 2-benzopyrans 2 and 3 was the Oxa-Pictet-Spengler reaction of the enantiomerically pure alcohols (R)-4 and (S)-4 with piperidone ketal 8 and chloropropionaldehyde acetal 12. The conformationally restricted spirocyclic 2-benzopyrans 2 revealed higher σ affinity than the more flexible aminoethyl derivatives 3. The (R)- and (R,R)-configured enantiomers (R)-2 and (R,R)-3 represent the eutomers of this class of compounds with eudismic ratios of 4.8 (2b) and 4.5 (2c). High σ /σ selectivity (>49) was found for the most potent σ ligands (R)-2b, (R)-2c, (R)-2d, and (S)-2d (K (σ ) 9-15nM).
[Mh] Termos MeSH primário: Benzopiranos/metabolismo
Lipase/metabolismo
[Mh] Termos MeSH secundário: Animais
Benzopiranos/química
Biocatálise
Burkholderia/enzimologia
Candida/enzimologia
Relação Dose-Resposta a Droga
Ligantes
Estrutura Molecular
Mucor/enzimologia
Pseudomonas fluorescens/enzimologia
Receptores sigma
Estereoisomerismo
Relação Estrutura-Atividade
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzopyrans); 0 (Ligands); 0 (Receptors, sigma); EC 3.1.1.3 (Lipase)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170803
[Lr] Data última revisão:
170803
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170515
[St] Status:MEDLINE


  9 / 1455 MEDLINE  
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[PMID]:28407499
[Au] Autor:Zahoor M; Irshad M; Rahman H; Qasim M; Afridi SG; Qadir M; Hussain A
[Ad] Endereço:Department of Botany, Garden Campus, Abdul Wali Khan University Mardan, Khyber Pakhtunkhwa, Pakistan.
[Ti] Título:Alleviation of heavy metal toxicity and phytostimulation of Brassica campestris L. by endophytic Mucor sp. MHR-7.
[So] Source:Ecotoxicol Environ Saf;142:139-149, 2017 Aug.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Heavy metal (HM) pollution is of great concern in countries like Pakistan where a huge proportion of human population is exposed to it. These toxic metals are making their way from water bodies to soil where it not only interferes with plant growth and development but also initiates serious health issues in human consuming the produce of such soils. Bioremediation is one of the most viable and efficient solution for the problem. Purpose of the current study was to isolate endophytic fungi from plants grown on HM contaminated soil and screen them for their ability to tolerate multiple HM including chromium (Cr ), manganese (Mn ), cobalt (Co ), copper (Cu ) and zinc (Zn ). Out of 27 isolated endophytes, only one strain (MHR-7) was selected for multiple heavy metals tolerance. The strain was identified as Mucor sp. by 18S and 28S ribosomal RNA internal transcribed spacer (ITS) 1 and 4 sequence homology. The strain effectively tolerated up to 900µgmL of these heavy metals showing no remarkable effect on its growth. The adverse effect of the heavy metals, measured as reduction of the fungal growth increased with increasing concentration of the metals. The strain was able to remove 60-87% of heavy metals from broth culture when supplied with 300µgmL of these metals. A trend of decline in bioremediation potential of the strain was observed with increasing amount of metals. The strain removed metals by biotransformation and/or accumulation of heavy metal in its hyphae. Application of Mucor sp. MHR-7 locked down HM in tis mycelium thereby making them less available to plant root reducing HM uptake and toxicity in mustard. Besides its bioremediation potential, the strain was also able to produce IAA, ACC deaminase and solubilize phosphate making it excellent phytostimulant fungus. It is concluded that MHR-7 is an excellent candidate for use as biofertilizer in fields affected with heavy metals.
[Mh] Termos MeSH primário: Brassica/efeitos dos fármacos
Endófitos/crescimento & desenvolvimento
Metais Pesados/toxicidade
Mucor/metabolismo
Poluentes do Solo/toxicidade
[Mh] Termos MeSH secundário: Biodegradação Ambiental
Brassica/metabolismo
Brassica/microbiologia
Hidrocarbonetos/metabolismo
Metais Pesados/análise
Metais Pesados/metabolismo
Mucor/efeitos dos fármacos
Mucor/isolamento & purificação
Paquistão
Desenvolvimento Vegetal/efeitos dos fármacos
Raízes de Plantas/efeitos dos fármacos
Raízes de Plantas/metabolismo
Raízes de Plantas/microbiologia
Solo/química
Poluentes do Solo/análise
Poluentes do Solo/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hydrocarbons); 0 (Metals, Heavy); 0 (Soil); 0 (Soil Pollutants)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170414
[St] Status:MEDLINE


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[PMID]:28355466
[Au] Autor:Bassetti M; Bouza E
[Ad] Endereço:Infectious Diseases Division, Santa Maria Misericordia University Hospital, Udine, Italy.
[Ti] Título:Invasive mould infections in the ICU setting: complexities and solutions.
[So] Source:J Antimicrob Chemother;72(suppl_1):i39-i47, 2017 Mar 01.
[Is] ISSN:1460-2091
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Infections caused by filamentous fungi represent a major burden in the ICU. Invasive aspergillosis is emerging in non-neutropenic individuals with predisposing conditions, e.g. corticosteroid treatment, chronic obstructive pulmonary disease, liver cirrhosis, solid organ cancer, HIV infection and transplantation. Diagnosis is challenging because the signs and symptoms are non-specific, and initiation of additional diagnostic examinations is often delayed because clinical suspicion is low. Isolation of an Aspergillus species from the respiratory tract in critically ill patients, and tests such as serum galactomannan, bronchoalveolar lavage 1-3-ß-d-glucan and specific PCR should be interpreted with caution. ICU patients should start adequate antifungal therapy upon suspicion of invasive aspergillosis, without awaiting definitive proof. Voriconazole, and now isavuconazole, are the drugs of choice. Mucormycosis is a rare, but increasingly prevalent disease that occurs mainly in patients with uncontrolled diabetes mellitus, immunocompromised individuals or previously healthy patients with open wounds contaminated with Mucorales. A high proportion of cases are diagnosed in the ICU. Rapidly progressing necrotizing lesions in the rhino-sinusal area, the lungs or skin and soft tissues are the characteristic presentation. Confirmation of diagnosis is based on demonstration of tissue invasion by non-septate hyphae, and by new promising molecular techniques. Control of underlying predisposing conditions, rapid surgical resection and administration of liposomal amphotericin B are the main therapeutic actions, but new agents such as isavuconazole are a promising alternative. Patients with mucormycosis receive a substantial part of their care in ICUs and, despite advances in diagnosis and treatment, mortality remains very high.
[Mh] Termos MeSH primário: Antifúngicos/uso terapêutico
Aspergilose/diagnóstico
Aspergilose/tratamento farmacológico
Infecções Fúngicas Invasivas/diagnóstico
Pneumopatias Fúngicas/diagnóstico
Mucormicose/diagnóstico
Mucormicose/tratamento farmacológico
Infecções Oportunistas/diagnóstico
[Mh] Termos MeSH secundário: Aspergilose/epidemiologia
Aspergillus/efeitos dos fármacos
Aspergillus/isolamento & purificação
Estado Terminal
Seres Humanos
Hospedeiro Imunocomprometido
Unidades de Terapia Intensiva
Infecções Fúngicas Invasivas/tratamento farmacológico
Infecções Fúngicas Invasivas/microbiologia
Pneumopatias Fúngicas/microbiologia
Mananas/sangue
Mucor/efeitos dos fármacos
Mucor/isolamento & purificação
Mucormicose/epidemiologia
Nitrilos/uso terapêutico
Infecções Oportunistas/tratamento farmacológico
Infecções Oportunistas/microbiologia
Piridinas/uso terapêutico
Sistema Respiratório/microbiologia
Triazóis/uso terapêutico
Voriconazol/uso terapêutico
beta-Glucanas/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Mannans); 0 (Nitriles); 0 (Pyridines); 0 (Triazoles); 0 (beta-Glucans); 11078-30-1 (galactomannan); 60UTO373KE (isavuconazole); 9051-97-2 (beta-1,3-glucan); JFU09I87TR (Voriconazole)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170330
[St] Status:MEDLINE
[do] DOI:10.1093/jac/dkx032



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