[PMID]: | 27341522 |
[Au] Autor: | Isah AA; Mahat NA; Jamalis J; Attan N; Zakaria II; Huyop F; Wahab RA |
[Ad] Endereço: | a Department of Chemistry, Faculty of Science , Universiti Teknologi Malaysia , Johor Bahru , Johor , Malaysia. |
[Ti] Título: | Synthesis of geranyl propionate in a solvent-free medium using Rhizomucor miehei lipase covalently immobilized on chitosan-graphene oxide beads. |
[So] Source: | Prep Biochem Biotechnol;47(2):199-210, 2017 Feb 07. |
[Is] ISSN: | 1532-2297 |
[Cp] País de publicação: | England |
[La] Idioma: | eng |
[Ab] Resumo: | The chemical route of producing geranyl propionate involves the use of toxic chemicals, liberation of unwanted by-products as well as problematic separation process. In view of such problems, the use of Rhizomucor miehei lipase (RML) covalently bound onto activated chitosan-graphene oxide (RML-CS/GO) support is suggested. Following analyses using Fourier transform infrared spectroscopy, field emission scanning electron microscopy, transmission electron microscopy, and thermogravimetry, properties of the RML-CS/GO were characterized. A response surface methodological approach using a 3-level-four-factor (incubation time, temperature, substrate molar ratio, and stirring rate) Box-Behnken design was used to optimize the experimental conditions to maximize the yield of geranyl propionate. Results revealed that 76 ± 0.02% of recovered protein had yielded 7.2 ± 0.04 mg g and 211 ± 0.3% U g of the maximum protein loading and esterification activity, respectively. The actual yield of geranyl propionate (49.46%) closely agreed with the predicted value (49.97%) under optimum reaction conditions (temperature: 37.67°C, incubation time: 10.20 hr, molar ratio (propionic acid:geraniol): 1:3.28, and stirring rate: 100.70 rpm) and hence, verifying the suitability of this approach. Since the method is performed under mild conditions, the RML-CS/GO biocatalyst may prove to be an environmentally benign alternative for producing satisfactory yield of geranyl propionate. |
[Mh] Termos MeSH primário: |
Quitosana/química Enzimas Imobilizadas/química Grafite/química Lipase/química Propionatos/síntese química Rhizomucor/enzimologia
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[Mh] Termos MeSH secundário: |
Meios de Cultura Microscopia Eletrônica de Varredura Microscopia Eletrônica de Transmissão Óxidos/química Solventes
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Culture Media); 0 (Enzymes, Immobilized); 0 (Oxides); 0 (Propionates); 0 (Solvents); 7782-42-5 (Graphite); 9012-76-4 (Chitosan); EC 3.1.1.3 (Lipase) |
[Em] Mês de entrada: | 1704 |
[Cu] Atualização por classe: | 170414 |
[Lr] Data última revisão:
| 170414 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 160625 |
[St] Status: | MEDLINE |
[do] DOI: | 10.1080/10826068.2016.1201681 |
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