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  1 / 2085 MEDLINE  
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[PMID]:27777036
[Au] Autor:Salgado-Lugo H; Sánchez-Arreguín A; Ruiz-Herrera J
[Ad] Endereço:Departamento de Ingeniería Genética, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Irapuato, Gto., Mexico.
[Ti] Título:Heterologous expression of an active chitin synthase from Rhizopus oryzae.
[So] Source:Fungal Genet Biol;97:10-17, 2016 12.
[Is] ISSN:1096-0937
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Chitin synthases are highly important enzymes in nature, where they synthesize structural components in species belonging to different eukaryotic kingdoms, including kingdom Fungi. Unfortunately, their structure and the molecular mechanism of synthesis of their microfibrilar product remain largely unknown, probably because no fungal active chitin synthases have been isolated, possibly due to their extreme hydrophobicity. In this study we have turned to the heterologous expression of the transcript from a small chitin synthase of Rhizopus oryzae (RO3G_00942, Chs1) in Escherichia coli. The enzyme was active, but accumulated mostly in inclusion bodies. High concentrations of arginine or urea solubilized the enzyme, but their dilution led to its denaturation and precipitation. Nevertheless, use of urea permitted the purification of small amounts of the enzyme. The properties of Chs1 (Km, optimum temperature and pH, effect of GlcNAc) were abnormal, probably because it lacks the hydrophobic transmembrane regions characteristic of chitin synthases. The product of the enzyme showed that, contrasting with chitin made by membrane-bound Chs's and chitosomes, was only partially in the form of short microfibrils of low crystallinity. This approach may lead to future developments to obtain active chitin synthases that permit understanding their molecular mechanism of activity, and microfibril assembly.
[Mh] Termos MeSH primário: Quitina Sintase/biossíntese
Quitina/biossíntese
Rhizopus/enzimologia
[Mh] Termos MeSH secundário: Arginina/química
Quitina/genética
Quitina Sintase/genética
Escherichia coli/genética
Regulação Fúngica da Expressão Gênica
Rhizopus/genética
Ureia/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
1398-61-4 (Chitin); 8W8T17847W (Urea); 94ZLA3W45F (Arginine); EC 2.4.1.16 (Chitin Synthase)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171214
[Lr] Data última revisão:
171214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161030
[St] Status:MEDLINE


  2 / 2085 MEDLINE  
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[PMID]:29049207
[Au] Autor:Cheng W; Wang G; Yang M; Sun L; Dong H; Chen Y; Cheng H
[Ad] Endereço:Division of Nephrology, Beijing Anzhen Hospital, Capital Medical University, Beijing, China.
[Ti] Título:Cutaneous mucormycosis in a patient with lupus nephritis: A case report and review of literature.
[So] Source:Medicine (Baltimore);96(42):e8211, 2017 Oct.
[Is] ISSN:1536-5964
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:RATIONALE: Mucormycosis is a rare fungal infection but life-threatening, especially in lupus nephritis (LN). Mucormycosis may manifest as rhino-orbital-cerebral, pulmonary, cutaneous, gastrointestinal, renal, or disseminated forms. PATIENT CONCERNS: We report a case of a 52-year-old woman with cutaneous mucormycosis infection who was admitted because of LN. DIAGNOSES: Histopathological analysis of the lesion confirmed the Rhizopus microspores from the family Mucoraceae. INTERVENTIONS AND OUTCOMES: The mortality of mucormycosis remains unacceptably high. Our patient died at last even with standard therapy (aggressive surgical debridement and anti-mucormycosis drugs). LESSONS: It is difficult to diagnose because lacking of specific clinical features, it is necessary to identify the susceptible patients, and then make diagnosis rapidly through tissue biopsy. Despite the outcome is poor, aggressive surgical debridement and Amphotericin B/Posaconazole can be effective.
[Mh] Termos MeSH primário: Dermatomicoses/microbiologia
Nefrite Lúpica/microbiologia
Mucormicose/microbiologia
[Mh] Termos MeSH secundário: Evolução Fatal
Feminino
Seres Humanos
Meia-Idade
Rhizopus/isolamento & purificação
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171122
[Lr] Data última revisão:
171122
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:171020
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000008211


  3 / 2085 MEDLINE  
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[PMID]:28847082
[Au] Autor:Liu H; Zhao S; Jin Y; Yue X; Deng L; Wang F; Tan T
[Ad] Endereço:Beijing Bioprocess Key Laboratory, State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, PR China.
[Ti] Título:Production of fumaric acid by immobilized Rhizopus arrhizus RH 7-13-9# on loofah fiber in a stirred-tank reactor.
[So] Source:Bioresour Technol;244(Pt 1):929-933, 2017 Nov.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Fumaric acid is an important building-block chemical. The production of fumaric acid by fermentation is possible. Loofah fiber is a natural, biodegradable, renewable polymer material with highly sophisticated and pore structure. This work investigated a new immobilization method using loofah fiber as carrier to produce fumaric acid in a stirred-tank reactor. Compared with other carriers, loofah fiber was proven to be efficiently and successfully used in the reactor. After the optimization process, 20g addition of loofah fiber and 400rpm agitation speed were chosen as the most suitable process conditions. 30.3g/L fumaric acid in the broth as well as 19.16g fumaric acid in the precipitation of solid was achieved, while the yield from glucose reached 0.211g/g. Three batches of fermentation using the same loofah fiber carrier were conducted successfully, which meant it provided a new method to produce fumaric acid in a stirred-tank reactor.
[Mh] Termos MeSH primário: Fumaratos
Rhizopus
[Mh] Termos MeSH secundário: Reatores Biológicos
Fermentação
Luffa
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fumarates); 88XHZ13131 (fumaric acid)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170830
[St] Status:MEDLINE


  4 / 2085 MEDLINE  
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[PMID]:28752804
[Au] Autor:Khaskheli AA; Talpur FN; Cebeci Aydin A; Jawaid S; Surhio MA; Afridi HI
[Ad] Endereço:a National Center of Excellence in Analytical Chemistry , University of Sindh , Jamshoro , Pakistan.
[Ti] Título:One-pot conjugated linoleic acid production from castor oil by Rhizopus oryzae lipase and resting cells of Lactobacillus plantarum.
[So] Source:Biosci Biotechnol Biochem;81(10):2002-2008, 2017 Oct.
[Is] ISSN:1347-6947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Conjugated linoleic acid (CLA) has attracted as novel type of fatty acids having unusual health-promoting properties such as anticarcinogenic and antiobesitic effects. The present work employed castor oil as substrate for one-pot production of CLA using washed cells of Lactobacillus plantarum (L. plantarum) and lipases as catalysts. Among the screened lipases, the lipase Rhizopus oryzae (ROL) greatly assisted resting cells to produce CLA. Mass spectral analysis of the product showed that two major isomers of CLA were produced in the reaction mixture i.e. cis-9, trans-11 56.55% and trans-10, cis-12 43.45%. Optimum factors for CLA synthesis were found as substrate concentration (8 mg/mL), pH (6.5), washed cell concentration (12% w/v), and incubation time of 20 h. Hence, the combination of ROL with L. plantarum offers one pot production of CLA selectively using castor oil as a cost-effective substrate.
[Mh] Termos MeSH primário: Biotecnologia/métodos
Óleo de Rícino/metabolismo
Lactobacillus plantarum/citologia
Lactobacillus plantarum/metabolismo
Ácidos Linoleicos Conjugados/biossíntese
Lipase/metabolismo
Rhizopus/enzimologia
[Mh] Termos MeSH secundário: Biotecnologia/economia
Análise Custo-Benefício
Concentração de Íons de Hidrogênio
Hidrólise
Cinética
Ácidos Linoleicos Conjugados/metabolismo
Probióticos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Linoleic Acids, Conjugated); 8001-79-4 (Castor Oil); EC 3.1.1.3 (Lipase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170729
[St] Status:MEDLINE
[do] DOI:10.1080/09168451.2017.1356218


  5 / 2085 MEDLINE  
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[PMID]:28681607
[Au] Autor:Yu XW; Yang M; Jiang C; Zhang X; Xu Y
[Ad] Endereço:The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University , Wuxi 214122, P. R. China.
[Ti] Título:N-Glycosylation Engineering to Improve the Constitutive Expression of Rhizopus oryzae Lipase in Komagataella phaffii.
[So] Source:J Agric Food Chem;65(29):6009-6015, 2017 Jul 26.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Our previous studies demonstrated that the N-glycans in Rhizopus chinensis lipase (RCL) was important for its secretion. In order to improve the secretion of Rhizopus oryzae lipase (ROL) under the control of the GAP promoter in Komagataella phaffii, two extra N-glycosylation sites were introduced in ROL according to the position of the N-glycosylation sites of RCL by sequence alignment. The results indicated that the secretion level of ROL was strongly improved by N-glycosylation engineering, and the highest value of extracellular enzyme activity was increased from 0.4 ± 0.2 U/mL to 207 ± 6 U/mL in a shake flask. In the 7-L fermenter, the extracellular enzyme activity of the mutant (2600 ± 43 U/mL) and the total protein concentration (2.5 ± 0.2 g/L) were 218- and 6.25-fold higher than these of the parent, respectively. This study presents a strategy for constitutive recombinant expression of ROL using the GAP promoter combined with N-glycosylation engineering, providing a potential enzyme for application in the food industry.
[Mh] Termos MeSH primário: Proteínas Fúngicas/genética
Proteínas Fúngicas/metabolismo
Lipase/genética
Lipase/metabolismo
Rhizopus/enzimologia
[Mh] Termos MeSH secundário: Proteínas Fúngicas/química
Expressão Gênica
Glicosilação
Lipase/química
Engenharia de Proteínas
Rhizopus/química
Rhizopus/genética
Saccharomycetales/genética
Saccharomycetales/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungal Proteins); EC 3.1.1.3 (Lipase)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170809
[Lr] Data última revisão:
170809
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170707
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b01884


  6 / 2085 MEDLINE  
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[PMID]:28673517
[Au] Autor:Zheng YX; Wang YL; Pan J; Zhang JR; Dai Y; Chen KY
[Ad] Endereço:Chongqing University, Chongqing 400044, China; Research Center for Tobacco Bioengineering and Technology of Chongqing Science and Technology Commission, Chongqing 401147, China; China Tobacco Chongqing Industrial Co. Ltd., Chongqing 400000, China.
[Ti] Título:Semi-continuous production of high-activity pectinases by immobilized Rhizopus oryzae using tobacco wastewater as substrate and their utilization in the hydrolysis of pectin-containing lignocellulosic biomass at high solid content.
[So] Source:Bioresour Technol;241:1138-1144, 2017 Oct.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In this study, highly reactive endo- and exo-polygalacturonases (PGs) were produced from the tobacco industry wastewater using immobilized Rhizopus oryzae. Compared with free cells, immobilized cells increased enzyme activity 2.8-fold and reduced production time to 24h by shake-flask production. Moreover, the immobilized cells enabled the semi-continuous production of enzymes through repeated-batch mode for seven consecutive cycles in a scale-up bioreactor. During the first five cycles, the average endo-PG and exo-PG activities reached 307.5 and 242.6U/ml, respectively. The addition of crude enzyme for the hydrolysis of pectin-containing lignocellulosic biomass under high-gravity conditions increased glucose release 4.2-fold (115.4 vs. 29.0g/L), compared with hydrolysis using cellulase alone. This process achieves the efficient production of pectin-degrading enzymes, provides a cost-effective method for tobacco wastewater treatment, and offers the possibility to obtain fermentable sugars with high-titer from pectin-containing lignocellulosic biomass, which has important potential for the commercial production of bio-fuels.
[Mh] Termos MeSH primário: Pectinas
Poligalacturonase
Rhizopus
Águas Residuais
[Mh] Termos MeSH secundário: Biomassa
Hidrólise
Tabaco
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Pectins); 0 (Waste Water); EC 3.2.1.15 (Polygalacturonase)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170705
[St] Status:MEDLINE


  7 / 2085 MEDLINE  
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[PMID]:28660762
[Au] Autor:Xia L; Bai Y; Mu W; Wang J; Xu X; Jin Z
[Ad] Endereço:State Key laboratory of Food Science and Technology, Jiangnan University , Wuxi, Jiangsu Province 214122, China.
[Ti] Título:Efficient Synthesis of Glucosyl-ß-Cyclodextrin from Maltodextrins by Combined Action of Cyclodextrin Glucosyltransferase and Amyloglucosidase.
[So] Source:J Agric Food Chem;65(29):6023-6029, 2017 Jul 26.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Instead of ß-cyclodextrin (ß-CD), branched ß-CDs have been increasingly used in many aspects as they possess better solubility and higher bioadaptability. But most commercialized branched ß-CDs were chemically synthesized. Thus, the glucosyl-ß-cyclodextrin (G -ß-CD) prepared via enzymatic approach could be a nice substitute. However, the yield of G -ß-CD was low. Here, we reported a controlled two-step reaction to efficiently prepare G -ß-CD from maltodextrins by ß-cyclodextrin glucosyltransferase (ß-CGTase) and amyloglucosidase (AG). Compared to the single ß-CGTase reaction, controlled two-step reaction caused a yield increase of G -ß-CD by 130%. Additionally, the percentage of G -ß-CD was enhanced from 2.4% to 24.0% and the side products α-CD and γ-CD were hydrolyzed because of the coupling activity of ß-CGTase. Thus, this controlled two-step reaction might be an efficient approach for industrial production of pure G -ß-CD.
[Mh] Termos MeSH primário: Proteínas de Bactérias/química
Proteínas Fúngicas/química
Glucana 1,4-alfa-Glucosidase/química
Glucosiltransferases/química
Polissacarídeos/química
beta-Ciclodextrinas/química
[Mh] Termos MeSH secundário: Proteínas de Bactérias/metabolismo
Biocatálise
Proteínas Fúngicas/metabolismo
Glucana 1,4-alfa-Glucosidase/metabolismo
Estrutura Molecular
Polissacarídeos/metabolismo
Rhizopus/enzimologia
Thermoanaerobacter/enzimologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Fungal Proteins); 0 (Polysaccharides); 0 (beta-Cyclodextrins); 7CVR7L4A2D (maltodextrin); EC 2.4.1.- (Glucosyltransferases); EC 2.4.1.19 (cyclomaltodextrin glucanotransferase); EC 3.2.1.3 (Glucan 1,4-alpha-Glucosidase)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170809
[Lr] Data última revisão:
170809
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170630
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b02079


  8 / 2085 MEDLINE  
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[PMID]:28591186
[Au] Autor:Guinea J; Escribano P; Vena A; Muñoz P; Martínez-Jiménez MDC; Padilla B; Bouza E
[Ad] Endereço:Clinical Microbiology and Infectious Diseases Department, Hospital General Universitario Gregorio Marañón, Universidad Complutense de Madrid, Madrid, Spain.
[Ti] Título:Increasing incidence of mucormycosis in a large Spanish hospital from 2007 to 2015: Epidemiology and microbiological characterization of the isolates.
[So] Source:PLoS One;12(6):e0179136, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We studied 19 cases of proven/probable mucormycosis diagnosed from 2007 to 2015 in our hospital and assessed the microbiological characteristics of the isolates. We recorded the incidence of mucormycosis and clinical and microbiological data of infected patients. Isolates were identified to molecular level and tested for their antifungal susceptibility to azoles, amphotericin B, and liposomal amphotericin B according to the CLSI M-38 A2 procedure. The incidence of mucormycosis in cases/100,000 hospital admissions during 2007-2015 increased significantly with respect to that reported in 1988-2006 (3.3 vs. 1.2; P<0.05). Patients mainly had hematological malignancies (52.6%) and/or trauma/surgical wounds (52.6%) and had received antifungal agents before the diagnosis of mucormycosis in 68% of cases. Diagnosis was by isolation (n = 17/19) and/or direct staining (n = 17/18) of Mucorales fungi in clinical samples. Identification was by panfungal PCR in patients with negative results in culture and in direct staining. The microorganisms identified were Lichtheimia spp. (42%), Rhizopus spp. (21%), Cunninghamella bertholletiae (16%), and others (21%). Liposomal amphotericin B was always more active than the other drugs against all the microorganisms except C. bertholletiae. All patients received antifungal treatment with 1 or more antifungal agents, mainly liposomal amphotericin B (17/19). Mortality was 47.4%, although this was significantly lower in the 11 patients in whom debridement was performed (18% vs. 87.5%) (P = 0.015). The incidence of mucormycosis has risen in recent years. The proportion of cases with soft tissue involvement was high, and Lichtheimia was the most frequently involved species. The highest antifungal activity was observed with liposomal amphotericin B.
[Mh] Termos MeSH primário: Neoplasias Hematológicas/epidemiologia
Mucormicose/tratamento farmacológico
Mucormicose/epidemiologia
Ferida Cirúrgica/epidemiologia
[Mh] Termos MeSH secundário: Adulto
Idoso
Anfotericina B/uso terapêutico
Antifúngicos/uso terapêutico
Azóis/uso terapêutico
Pré-Escolar
Cunninghamella/isolamento & purificação
Cunninghamella/patogenicidade
Feminino
Neoplasias Hematológicas/complicações
Neoplasias Hematológicas/microbiologia
Neoplasias Hematológicas/cirurgia
Seres Humanos
Masculino
Meia-Idade
Mucormicose/complicações
Mucormicose/microbiologia
Rhizopus/isolamento & purificação
Rhizopus/patogenicidade
Ferida Cirúrgica/complicações
Ferida Cirúrgica/tratamento farmacológico
Ferida Cirúrgica/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Azoles); 0 (liposomal amphotericin B); 7XU7A7DROE (Amphotericin B)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170913
[Lr] Data última revisão:
170913
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170608
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179136


  9 / 2085 MEDLINE  
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[PMID]:28420334
[Au] Autor:Song Y; Qiao J; Giovanni G; Liu G; Yang H; Wu J; Chen J
[Ad] Endereço:Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, No. 79 Qingchun Road, Hangzhou, 310003, Zhejiang, People's Republic of China.
[Ti] Título:Mucormycosis in renal transplant recipients: review of 174 reported cases.
[So] Source:BMC Infect Dis;17(1):283, 2017 Apr 18.
[Is] ISSN:1471-2334
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Mucormycosis is a highly lethal fungal infection especially in immunocompromised individuals. METHODS: In order to review the epidemiology, diagnosis, and treatment of mucormycosis in renal transplant recipients we searched publications of mucormycosis cases in renal transplant recipients in PUBMED database up to December 2015. RESULTS: A total of 174 cases in renal transplant recipients were included in this review. Most of the cases (76%) were male. Major underlying diseases were diabetes mellitus (43.1%). Rhinocerebral was the most common site of infection (33.3%). Rhizopus species was the most frequent fungus (59.1%) in patients with pathogen identified to species level. The mortality rates of disseminated mucormycosis (76.0%) and graft renal (55.6%) were higher than infection in other sites. The overall survival in patients received surgical debridement combined with amphotericin B/posaconazole (70.2%) was higher than those who received antifungal therapy alone (32.4%), surgery alone (36.4%) or without therapy (0%) (p < 0.001). The overall survivals in patients receiving posaconazole and lipid amphoterincin B were higher than that receiving deoxycholate formulation (92.3% and 73.4% vs 47.4%). CONCLUSIONS: Mucormycosis is a severe infection in renal transplant recipients. Surgical debridement combined with antifungals, especially liposomal amphotericin B and posaconazole, can significantly improve patient's overall survival.
[Mh] Termos MeSH primário: Antifúngicos/uso terapêutico
Transplante de Rim/efeitos adversos
Mucormicose/etiologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Anfotericina B/uso terapêutico
Criança
Desbridamento
Ácido Desoxicólico
Diabetes Mellitus
Feminino
Seres Humanos
Hospedeiro Imunocomprometido
Masculino
Meia-Idade
Mucormicose/tratamento farmacológico
Mucormicose/mortalidade
Rhizopus/patogenicidade
Transplantados
Triazóis/uso terapêutico
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Triazoles); 0 (liposomal amphotericin B); 005990WHZZ (Deoxycholic Acid); 6TK1G07BHZ (posaconazole); 7XU7A7DROE (Amphotericin B)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170522
[Lr] Data última revisão:
170522
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170420
[St] Status:MEDLINE
[do] DOI:10.1186/s12879-017-2381-1


  10 / 2085 MEDLINE  
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[PMID]:28352177
[Au] Autor:Machado MC; Webster TJ
[Ad] Endereço:Center for Biomedical Engineering, Division of Engineering Brown University, Providence, RI.
[Ti] Título:Lipase degradation of plasticized polyvinyl chloride endotracheal tube surfaces to create nanoscale features.
[So] Source:Int J Nanomedicine;12:2109-2115, 2017.
[Is] ISSN:1178-2013
[Cp] País de publicação:New Zealand
[La] Idioma:eng
[Ab] Resumo:Polyvinyl chloride (PVC) endotracheal tubes (ETTs) nanoetched with a fungal lipase have been shown to reduce bacterial growth and biofilm formation and could be an inexpensive solution to the complex problem of ventilator-associated pneumonia (VAP). Although bacterial growth and colonization on these nanoetched materials have been well characterized, little is known about the mechanism by which the fungal lipase degrades the PVC and, thus, alters its properties to minimize bacteria functions. This study used X-ray photoelectron spectroscopy (XPS) and attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) to better describe the surface chemistry of both unetched and lipase nanoetched PVC ETT. ATR-FTIR analysis of the unetched and treated surfaces showed a similar presence of a plasticizer. This was confirmed by XPS analysis, which showed an increase of carbon and the presence of oxygen on both unetched and nanoetched surfaces. A quantitative comparison of the FTIR spectra revealed significant correlations (Pearson's correlation, =0.997 [ =0.994, <0.001]) between the unetched and nanomodified PVC ETT spectra, demonstrating similar surface chemistry. This analysis showed no shifting or widening of the bands in the spectra and no significant changes in the intensity of the infrared peaks due to the degradation of the plasticizer by the fungal lipase. In contrast, results from this study did demonstrate significantly increased nanoscale surface features on the lipase etched compared to non-etched PVC ETTs. This led to a change in surface energetics, which altered ion adsorption to the ETTs. Thus, these results showed that PVC surfaces nanoetched with a 0.1% lipase solution for 48 hours have no significant change on surface chemistry but do significantly increase nanoscale surface roughness and alters ion adsorption, which suggests that the unique properties of these materials, including their previously reported ability to decrease bacterial adhesion and growth, are due to the changes in the degree of the nanoscale roughness, not changes in their surface chemistry.
[Mh] Termos MeSH primário: Intubação Intratraqueal
Lipase/metabolismo
Nanopartículas/química
Plastificantes/farmacologia
Cloreto de Polivinila/farmacologia
Proteólise/efeitos dos fármacos
[Mh] Termos MeSH secundário: Hidrólise
Espectroscopia Fotoeletrônica
Rhizopus/enzimologia
Espectroscopia de Infravermelho com Transformada de Fourier
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plasticizers); 9002-86-2 (Polyvinyl Chloride); EC 3.1.1.3 (Lipase)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170522
[Lr] Data última revisão:
170522
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170330
[St] Status:MEDLINE
[do] DOI:10.2147/IJN.S130608



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