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[PMID]:28749993
[Au] Autor:Chen G; Wang W; Chen H; Dai W; Peng X; Li X; Tang X; Xu L; Shen Z
[Ad] Endereço:Jiangsu University of Science and Technology, Zhenjiang, Jiangsu Province, China.
[Ti] Título:Functional characterization of an aquaporin from a microsporidium, Nosema bombycis.
[So] Source:PLoS One;12(7):e0181703, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Microsporidia are a diverse group of eukaryotic organisms, capable of causing parasitic infections in both vertebrates and invertebrates. During the germination process, there is an increase in the osmotic pressure of microsporidian spores. As part of this study, we cloned a homologous aquaporin gene in Nosema bombycis, and named it Nosema bombycis aquaporin (NbAQP). Sequence analysis revealed that the NbAQP contains an open reading frame with a length of 750 bp and encodes a polypeptide of 249 amino acids. Amino acid sequence homology was greater than 50% that of five aquaporins from other microsporidian species. Indirect immunofluorescence (IFA) and immunogold electron microscopy showed NbAQP to be located predominantly in the spore wall of N. bombycis spores. The results of qRT-PCR analysis revealed that NbAQP expression remained high 0 h after inoculation and decreased sharply to 24 h, increased gradually from 2 days and peaked at 6 days. After expression of NbAQP in Xenopus laevis oocytes, it was observed that NbAQP can promote rapid penetration of water into oocytes. The associated permeation rate was 2-3 times that of the water-injected and uninjected oocytes. Antibody blocking experiments showed that the inhibition rate of spore germination was approximately 28% after antibody blocking. The difference in germination rate between the control group and the NbAQP group was significant (P < 0.05). This study shows for the first time that N. bombycis contains functional water channel proteins and provides a platform suitable for further research into the mechanisms underlying the regulation of NbAQP protein expression. Further study of NbAQP and their inhibitors may have significance for prevention of microsporidiosis.
[Mh] Termos MeSH primário: Aquaporinas/fisiologia
Proteínas Fúngicas/fisiologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Anticorpos Antifúngicos/farmacologia
Antifúngicos/farmacologia
Aquaporinas/antagonistas & inibidores
Células Cultivadas
Proteínas Fúngicas/antagonistas & inibidores
Modelos Moleculares
Nosema/efeitos dos fármacos
Nosema/metabolismo
Filogenia
Domínios Proteicos
Coelhos
Homologia de Sequência de Aminoácidos
Esporos Fúngicos/efeitos dos fármacos
Esporos Fúngicos/metabolismo
Xenopus laevis
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Fungal); 0 (Antifungal Agents); 0 (Aquaporins); 0 (Fungal Proteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170928
[Lr] Data última revisão:
170928
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170728
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0181703


  2 / 476 MEDLINE  
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[PMID]:28663099
[Au] Autor:Brown MJF
[Ad] Endereço:School of Biological Sciences, Royal Holloway University of London, Egham, TW20 0EX, UK. Electronic address: mark.brown@rhul.ac.uk.
[Ti] Título:Microsporidia: An Emerging Threat to Bumblebees?
[So] Source:Trends Parasitol;33(10):754-762, 2017 10.
[Is] ISSN:1471-5007
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Microsporidia may cause emerging infectious diseases (EIDs) in bumblebees. Two drivers - commercial bumblebees and managed honey bees - have been identified as possible sources of pathogen spillover. In addition, declines in bumblebee populations may have led to lower genetic diversity and subsequent higher susceptibility to infection, enabling microsporidia to increase in prevalence. There is strong evidence for relatively recent increases in the prevalence of Nosema bombi in North America. However, the lack of definitive data on spillover by microsporidia, in North America or elsewhere, makes it difficult to identify the causes of such increases. Phylogenomic studies are urgently needed to identify the global population structure of microsporidia in bumblebees, and thus identify the source of current and future epidemics.
[Mh] Termos MeSH primário: Abelhas/genética
Abelhas/microbiologia
Nosema/fisiologia
[Mh] Termos MeSH secundário: Animais
Variação Genética
América do Norte
Nosema/classificação
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171121
[Lr] Data última revisão:
171121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170701
[St] Status:MEDLINE


  3 / 476 MEDLINE  
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[PMID]:28640848
[Au] Autor:Chen J; Guo W; Dang X; Huang Y; Liu F; Meng X; An Y; Long M; Bao J; Zhou Z; Xiang Z; Pan G
[Ad] Endereço:State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, P. R. China.
[Ti] Título:Easy labeling of proliferative phase and sporogonic phase of microsporidia Nosema bombycis in host cells.
[So] Source:PLoS One;12(6):e0179618, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Microsporidia are eukaryotic, unicellular parasites that have been studied for more than 150 years. These organisms are extraordinary in their ability to invade a wide range of hosts including vertebrates and invertebrates, such as human and commercially important animals. A lack of appropriate labeling methods has limited the research of the cell cycle and protein locations in intracellular stages. In this report, an easy fluorescent labeling method has been developed to mark the proliferative and sporogonic phases of microsporidia Nosema bombycis in host cells. Based on the presence of chitin, Calcofluor White M2R was used to label the sporogonic phase, while ß-tubulin antibody coupled with fluorescence secondary antibody were used to label the proliferative phase by immunofluorescence. This method is simple, efficient and can be used on both infected cells and tissue slices, providing a great potential application in microsporidia research.
[Mh] Termos MeSH primário: Nosema/fisiologia
Esporos Fúngicos/fisiologia
[Mh] Termos MeSH secundário: Regulação Fúngica da Expressão Gênica
Espaço Intracelular/metabolismo
Nosema/citologia
Nosema/genética
Tubulina (Proteína)/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Tubulin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170623
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179618


  4 / 476 MEDLINE  
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[PMID]:28350872
[Au] Autor:Badaoui B; Fougeroux A; Petit F; Anselmo A; Gorni C; Cucurachi M; Cersini A; Granato A; Cardeti G; Formato G; Mutinelli F; Giuffra E; Williams JL; Botti S
[Ad] Endereço:Parco Tecnologico Padano - CERSA, Integrative Biology Group, Lodi, Italy.
[Ti] Título:RNA-sequence analysis of gene expression from honeybees (Apis mellifera) infected with Nosema ceranae.
[So] Source:PLoS One;12(3):e0173438, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Honeybees (Apis mellifera) are constantly subjected to many biotic stressors including parasites. This study examined honeybees infected with Nosema ceranae (N. ceranae). N. ceranae infection increases the bees energy requirements and may contribute to their decreased survival. RNA-seq was used to investigate gene expression at days 5, 10 and 15 Post Infection (P.I) with N. ceranae. The expression levels of genes, isoforms, alternative transcription start sites (TSS) and differential promoter usage revealed a complex pattern of transcriptional and post-transcriptional gene regulation suggesting that bees use a range of tactics to cope with the stress of N. ceranae infection. N. ceranae infection may cause reduced immune function in the bees by: (i)disturbing the host amino acids metabolism (ii) down-regulating expression of antimicrobial peptides (iii) down-regulation of cuticle coatings and (iv) down-regulation of odorant binding proteins.
[Mh] Termos MeSH primário: Abelhas/genética
Abelhas/microbiologia
Perfilação da Expressão Gênica/métodos
Nosema/fisiologia
Análise de Sequência de RNA/métodos
[Mh] Termos MeSH secundário: Animais
Metabolismo Energético/genética
Genes de Insetos/genética
Interações Hospedeiro-Patógeno
Proteínas de Insetos/genética
Análise de Componente Principal
Regiões Promotoras Genéticas/genética
Isoformas de Proteínas/genética
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Transdução de Sinais/genética
Esporos Fúngicos/fisiologia
Fatores de Tempo
Sítio de Iniciação de Transcrição
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insect Proteins); 0 (Protein Isoforms)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170828
[Lr] Data última revisão:
170828
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170329
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0173438


  5 / 476 MEDLINE  
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[PMID]:28338834
[Au] Autor:Belkorchia A; Pombert JF; Polonais V; Parisot N; Delbac F; Brugère JF; Peyret P; Gaspin C; Peyretaillade E
[Ad] Endereço:Laboratoire "Microorganismes: Génome et Environnement", Université Clermont Auvergne, BP 10448, F-63000 Clermont-Ferrand, France.
[Ti] Título:Comparative genomics of microsporidian genomes reveals a minimal non-coding RNA set and new insights for transcription in minimal eukaryotic genomes.
[So] Source:DNA Res;24(3):251-260, 2017 Jun 01.
[Is] ISSN:1756-1663
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Microsporidia are ubiquitous intracellular pathogens whose opportunistic nature led to their increased recognition with the rise of the AIDS pandemic. As the RNA world was largely unexplored in this parasitic lineage, we developed a dedicated in silico methodology to carry out exhaustive identification of ncRNAs across the Encephalitozoon and Nosema genera. Thus, the previously missing U1 small nuclear RNA (snRNA) and small nucleolar RNAs (snoRNAs) targeting only the LSU rRNA were highlighted and were further validated using 5' and 3'RACE-PCR experiments. Overall, the 15 ncRNAs that were found shared between Encephalitozoon and Nosema spp. may represent the minimal core set required for parasitic life. Interestingly, the systematic presence of a CCC- or GGG-like motif in 5' of all ncRNA and mRNA gene transcripts regardless of the RNA polymerase involved suggests that the RNA polymerase machineries in microsporidia species could use common factors. Our data provide additional insights in accordance with the simplification processes observed in these reduce genomes and underline the usefulness of sequencing closely related species to help identify highly divergent ncRNAs in these parasites.
[Mh] Termos MeSH primário: Encephalitozoon/genética
Genoma Fúngico
Nosema/genética
RNA não Traduzido/metabolismo
Transcrição Genética
[Mh] Termos MeSH secundário: Sequência de Bases
Simulação por Computador
Genômica
RNA Nuclear Pequeno/metabolismo
RNA Nucleolar Pequeno/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Small Nuclear); 0 (RNA, Small Nucleolar); 0 (RNA, Untranslated); 0 (U1 small nuclear RNA)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170807
[Lr] Data última revisão:
170807
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170325
[St] Status:MEDLINE
[do] DOI:10.1093/dnares/dsx002


  6 / 476 MEDLINE  
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[PMID]:28152065
[Au] Autor:Martín-Hernández R; Higes M; Sagastume S; Juarranz Á; Dias-Almeida J; Budge GE; Meana A; Boonham N
[Ad] Endereço:Laboratorio de Patología Apícola, Centro de Investigación Apícola y Agroambiental, IRIAF, Consejería de Agricultura de la Junta de Comunidades de Castilla-La Mancha, Marchamalo, Spain.
[Ti] Título:Microsporidia infection impacts the host cell's cycle and reduces host cell apoptosis.
[So] Source:PLoS One;12(2):e0170183, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Intracellular parasites can alter the cellular machinery of host cells to create a safe haven for their survival. In this regard, microsporidia are obligate intracellular fungal parasites with extremely reduced genomes and hence, they are strongly dependent on their host for energy and resources. To date, there are few studies into host cell manipulation by microsporidia, most of which have focused on morphological aspects. The microsporidia Nosema apis and Nosema ceranae are worldwide parasites of honey bees, infecting their ventricular epithelial cells. In this work, quantitative gene expression and histology were studied to investigate how these two parasites manipulate their host's cells at the molecular level. Both these microsporidia provoke infection-induced regulation of genes involved in apoptosis and the cell cycle. The up-regulation of buffy (which encodes a pro-survival protein) and BIRC5 (belonging to the Inhibitor Apoptosis protein family) was observed after infection, shedding light on the pathways that these pathogens use to inhibit host cell apoptosis. Curiously, different routes related to cell cycle were modified after infection by each microsporidia. In the case of N. apis, cyclin B1, dacapo and E2F2 were up-regulated, whereas only cyclin E was up-regulated by N. ceranae, in both cases promoting the G1/S phase transition. This is the first report describing molecular pathways related to parasite-host interactions that are probably intended to ensure the parasite's survival within the cell.
[Mh] Termos MeSH primário: Abelhas/microbiologia
Interações Hospedeiro-Patógeno/fisiologia
Microsporídios/patogenicidade
Nosema/patogenicidade
[Mh] Termos MeSH secundário: Animais
Apoptose/genética
Abelhas/citologia
Abelhas/genética
Ciclo Celular/genética
Genes de Insetos
Interações Hospedeiro-Patógeno/genética
Microsporidiose/microbiologia
Microsporidiose/patologia
Modelos Genéticos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170814
[Lr] Data última revisão:
170814
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170203
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0170183


  7 / 476 MEDLINE  
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[PMID]:28085231
[Au] Autor:Plischuk S; Antúnez K; Haramboure M; Minardi GM; Lange CE
[Ad] Endereço:Centro de Estudios Parasitológicos y de Vectores (CONICET - UNLP), La Plata, Argentina.
[Ti] Título:Long-term prevalence of the protists Crithidia bombi and Apicystis bombi and detection of the microsporidium Nosema bombi in invasive bumble bees.
[So] Source:Environ Microbiol Rep;9(2):169-173, 2017 Apr.
[Is] ISSN:1758-2229
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:An initial survey in 2009 carried out at a site in northwestern Patagonia region, Argentina, revealed for the first time in South America the presence of the flagellate Crithidia bombi and the neogregarine Apicystis bombi, two pathogens associated with the Palaearctic invasive bumble bee Bombus terrestris. In order to determine the long-term persistence and dynamics of this microparasite complex, four additional collections at the same site (San Carlos de Bariloche) were conducted along the following seven years. Both protists were detected in all collections: prevalence was 2%-21.6% for C. bombi and 1.2%-14% for A. bombi. In addition, the microsporidium Nosema bombi was recorded for the first time in the country in the last two collections, at prevalences of 12.4% and 2.4% and unusually high infection intensities (Average = 6.56 × 10 spores per individual). Due to the exceptional dispersal ability of the exotic B. terrestris, these three multihost pathogens should be considered as potential threats to South American native bumble bees.
[Mh] Termos MeSH primário: Apicomplexa/isolamento & purificação
Abelhas/microbiologia
Abelhas/parasitologia
Crithidia/isolamento & purificação
Nosema/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Argentina
Prevalência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170829
[Lr] Data última revisão:
170829
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170114
[St] Status:MEDLINE
[do] DOI:10.1111/1758-2229.12520


  8 / 476 MEDLINE  
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[PMID]:28031263
[Au] Autor:Yang D; Pan L; Peng P; Dang X; Li C; Li T; Long M; Chen J; Wu Y; Du H; Luo B; Song Y; Tian R; Luo J; Zhou Z; Pan G
[Ad] Endereço:State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, People's Republic of China.
[Ti] Título:Interaction between SWP9 and Polar Tube Proteins of the Microsporidian Nosema bombycis and Function of SWP9 as a Scaffolding Protein Contribute to Polar Tube Tethering to the Spore Wall.
[So] Source:Infect Immun;85(3), 2017 Mar.
[Is] ISSN:1098-5522
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:All microsporidia possess a unique, highly specialized invasion mechanism that involves the polar tube and spore wall. The interaction between spore wall proteins (SWPs) and polar tube proteins (PTPs) in the formation, arrangement, orderly orientation, and function of the polar tube and spore wall remains to be determined. This study was undertaken to examine the protein interactions of SWP7 (NbSWP7), NbSWP9, and PTPs. Coimmunoprecipitation, liquid chromatography-tandem mass spectrometry (LC-MS/MS), and yeast two-hybrid data demonstrated that NbSWP9, but not NbSWP7, interacts with NbPTP1 and NbPTP2. Furthermore, immunoelectron microscopy (IEM) showed that NbSWP9 was localized mainly in the developing polar tube of sporoblasts, while NbSWP7 was found randomly in the cytoplasm. However, both NbSWP9 and NbSWP7 were located in the polar tube and spore wall of mature spores. The reason why NbSWP7 was localized to the polar tube may be due to the interaction between NbSWP9 and NbSWP7. Interestingly, the majority of NbSWP9, but not NbSWP7, accumulated in the beginning part of the extruded polar tube and the ruptured spore wall called the anchoring disk (AD) when the mature spores germinated under weak-alkaline environmental stimulation. Additionally, anti-NbSWP9 antibody reduced spore germination in a dose-dependent manner. In conclusion, our study further confirmed that NbSWP9 is a scaffolding protein that not only anchors and holds the polar tube but also tethers the polar tube to the spore wall.
[Mh] Termos MeSH primário: Proteínas Fúngicas/metabolismo
Nosema/fisiologia
Esporos Fúngicos
[Mh] Termos MeSH secundário: Parede Celular/metabolismo
Nosema/ultraestrutura
Ligação Proteica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungal Proteins)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170824
[Lr] Data última revisão:
170824
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161230
[St] Status:MEDLINE


  9 / 476 MEDLINE  
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[PMID]:28025388
[Au] Autor:Mendoza Y; Diaz-Cetti S; Ramallo G; Santos E; Porrini M; Invernizzi C
[Ad] Endereço:Instituto Nacional de Investigación Agropecuaria, Apicultura, Uruguay.
[Ti] Título:Nosema ceranae Winter Control: Study of the Effectiveness of Different Fumagillin Treatments and Consequences on the Strength of Honey Bee (Hymenoptera: Apidae) Colonies.
[So] Source:J Econ Entomol;110(1):1-5, 2017 02 01.
[Is] ISSN:1938-291X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Overview: In Uruguay, colonies of honey bees moving to Eucalyptus grandis plantation in autumn habitually become infected with the microsporidian Nosema ceranae , a parasite that attacks the digestive system of bees. Beekeepers attributed to N. ceranae depopulation of the colonies that often occurs at the end of the blooming period, and many use the antibiotic fumagillin to reduce the level of infection. The aim of this study was to compare the effectiveness of four different fumagillin treatments and determine how this antibiotic affects the strength of the colonies during the winter season. The colonies treated with fumagillin in July showed less spore load at the end of applications, being the most effective the following treatments: the four applications sprayed over bees of 30 mg of fumagillin in 100 ml of sugar syrup 1:1, and four applications of 90 mg of fumagillin in 250 ml of sugar syrup 1:1 using a feeder. However, 2 month after the treatment applications, the colonies treated with fumagillin were the same size as the untreated colonies. In September, the colonies treated and not treated with fumagillin did not differ in colony strength (adult bee population and brood area) or spores abundance. Our study demonstrates that fumagillin treatment temporarily decreased the spore load of N. ceranae , but this was not reflected in either the size of the colonies or the probability of surviving the winter regardless of the dose or the administration strategy applied. Given the results obtained, we suggest to not perform the pharmacological treatment under the conditions described in the experiment. Resumen: En Uruguay las colonias de abejas melíferas que se trasladan a las forestaciones de Eucalyptus grandis en otoño indefectiblemente se infectan con el microsporido Nosema ceranae , parásito que ataca el sistema digestivo de las abejas. Los apicultores atribuyen a N. ceranae el despoblamiento de las colonias que ocurre con frecuencia al terminar el periodo de floración y muchos emplean el antibiótico fumagilina para reducir el nivel de infección. El objetivo de este estudio fue comparar la eficacia de cuatro tratamientos diferentes con fumagilina y determinar cómo incide en la fortaleza de las colonias durante la invernada. Las colonias tratadas con fumagilina en julio presentaron una menor carga de esporas al terminar las aplicaciones, siendo los tratamientos más eficaces el de 4 aplicaciones mediante asperjado sobre las abejas de 30 mg de fumagilina en 100 ml de jarabe de azúcar 1:1, y el de 4 aplicaciones de 90 mg de fumagilina en 250 ml de jarabe de azúcar 1:1 utilizando un alimentador. Sin embargo, durante el período de experimentación, las colonias tratadas con antibiótico presentaron igual tamaño que las colonias no tratadas. En setiembre, las colonias tratadas y no tratadas con fumagilina no se diferenciaron en la intensidad de infección ni en su tamaño. En las condiciones en que se realizó el estudio, la aplicación de fumagilina disminuyó temporalmente la carga de esporas de N. ceranae pero esto no se reflejó en el tamaño de las colonias ni en la probabilidad de sobrevivir el invierno.
[Mh] Termos MeSH primário: Abelhas/microbiologia
Abelhas/fisiologia
Cicloexanos/farmacologia
Ácidos Graxos Insaturados/farmacologia
Fungicidas Industriais/farmacologia
Nosema/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Relação Dose-Resposta a Droga
Eucalyptus
Nosema/fisiologia
Dinâmica Populacional
Estações do Ano
Sesquiterpenos/farmacologia
Uruguai
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cyclohexanes); 0 (Fatty Acids, Unsaturated); 0 (Fungicides, Industrial); 0 (Sesquiterpenes); 7OW73204U1 (fumagillin)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170823
[Lr] Data última revisão:
170823
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161228
[St] Status:MEDLINE
[do] DOI:10.1093/jee/tow228


  10 / 476 MEDLINE  
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[PMID]:27989634
[Au] Autor:Timofeev SA; Senderskiy IV; Tsarev AA; Tokarev YS; Dolgikh VV
[Ad] Endereço:Laboratory of Microbiological Control, All-Russian Institute for Plant Protection, St. Petersburg, Pushkin, Russia.
[Ti] Título:Heterologous expression of Paranosema (Antonospora) locustae hexokinase in lepidopteran, Sf9, cells is followed by accumulation of the microsporidian protein in insect cell nuclei.
[So] Source:J Invertebr Pathol;143:104-107, 2017 Feb.
[Is] ISSN:1096-0805
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Paranosema (Nosema, Antonospora) locustae is the only microsporidium produced as a commercial product for biological control. Molecular mechanisms of the effects of this pathogen and other invertebrate microsporidia on host cells remain uncharacterized. Previously, we immunolocalized P. locustae hexokinase in nuclei of Locusta migratoria infected adipocytes. Here, the microsporidian protein was expressed in the yeast Pichia pastoris and in lepidopteran Sf9 cells. During heterologous expression, P. locustae hexokinase was accumulated in the nuclei of insect cells but not in yeast cell nuclei. This confirms nuclear localization of hexokinase secreted by microsporidia into infected host cells and suggests convenient model for its further study.
[Mh] Termos MeSH primário: Proteínas Fúngicas/biossíntese
Hexoquinase/biossíntese
Nosema/enzimologia
Spodoptera/parasitologia
[Mh] Termos MeSH secundário: Animais
Núcleo Celular/metabolismo
Microsporidiose/veterinária
Pichia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungal Proteins); EC 2.7.1.1 (Hexokinase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171118
[Lr] Data última revisão:
171118
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161220
[St] Status:MEDLINE



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