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[PMID]:29107194
[Au] Autor:Guimarães A; Santiago A; Teixeira JA; Venâncio A; Abrunhosa L
[Ad] Endereço:CEB - Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal.
[Ti] Título:Anti-aflatoxigenic effect of organic acids produced by Lactobacillus plantarum.
[So] Source:Int J Food Microbiol;264:31-38, 2018 Jan 02.
[Is] ISSN:1879-3460
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Lactic acid bacteria (LAB), which are commonly used in the production of fermented foods, have been gaining attention for their antifungal and antimycotoxin properties. In this work, the strain Lactobacillus plantarum UM55 was selected among other LAB for inhibiting the growth of Aspergillus flavus. Further, it is shown that cell-free supernatant (CFS) of this strain inhibits the production of aflatoxins (AFLs) by 91%. This inhibition was dependent on CFS pH, increased with increasing concentrations of CFS, and was independent of fungal growth, which was inhibited only by 32%. CFS was also effective in inhibiting the growth and AFLs production in A. parasiticus, A. arachidicola, A. nomius and A. minisclerotigenes. Further, L. plantarum UM55 CFS was analysed for the presence of organic acids and the main differences compared to controls were found in the levels of lactic acid, phenyllactic acid (PLA), hydroxyphenyllactic acid (OH-PLA), and indole lactic acid (ILA). These compounds were individually tested against A. flavus, with all of the compounds showing an inhibiting effect on fungal growth and AFLs production. PLA showed the stronger effects, and the obtained IC for the inhibition of growth and AFLs was of 11.9 and 0.87mg/mL, respectively. AFLs IC for ILA, OH-PLA and lactic acid were of 1.47, 1.80, and 3.92mg/mL, respectively. The antiaflatoxigenic properties of LAB depend on strain's capability to produce lactic acid, PLA, OH-PLA and ILA.
[Mh] Termos MeSH primário: Aflatoxinas/antagonistas & inibidores
Aspergillus flavus/crescimento & desenvolvimento
Aspergillus flavus/metabolismo
Ácido Láctico/metabolismo
Lactobacillus plantarum/fisiologia
[Mh] Termos MeSH secundário: Antifúngicos/metabolismo
Agentes de Controle Biológico/metabolismo
Ácido Láctico/análogos & derivados
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aflatoxins); 0 (Antifungal Agents); 0 (Biological Control Agents); 33X04XA5AT (Lactic Acid)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171107
[St] Status:MEDLINE


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[PMID]:29244880
[Au] Autor:Shekhar M; Singh N; Dutta R; Kumar S; Mahajan V
[Ad] Endereço:ICAR-Indian Institute of Maize Research (IIMR), Pusa Campus, New Delhi, India.
[Ti] Título:Comparative study of qualitative and quantitative methods to determine toxicity level of Aspergillus flavus isolates in maize.
[So] Source:PLoS One;12(12):e0189760, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:An attempt was made to compare between easy and inexpensive qualitative method (ammonia vapour test) and analytical methods (thin layer chromatography and enzyme-linked immunosorbent assay) for identification of aflatoxigenic isolates of Aspergillus flavus in maize. In this comparative study the toxicity level of A. flavus isolates exhibited 100% agreement among ammonia vapour test, ELISA and TLC for highly toxigenic (>2000 ppb) and toxigenic (501-2000 ppb) isolates while 88.5% agreement observed for least toxic (<20 ppb) isolates. In ammonia vapour test 51% of A. flavus isolates showed creamish or no colour change corresponding to least toxic/atoxic (<20ppb) category estimated by ELISA. Similarly 22% highly toxic isolates exhibited plum red colour, 12% moderately toxic indicated pink colour and 10% toxic isolates showed red colour. However, 11.5% isolates were found to be false positive in cream colour category (least toxic) and 28.5% false negatives in pink colour (moderately toxic) category. The isolates from different agroclimatic zones of maize in India showed high variability for aflatoxin B1 (AFB1) production potential ranging from 0.214-8116.61 ppb. Toxigenic potential of Aspergillus flavus isolates in culture was further validated by inoculating maize grain sample with four different isolates with varied toxin producing ability. With good agreement percentage between cultural and analytical methods the study concludes the ammonia vapour test to be easy, inexpensive, reliable and time saving method that can be used for segregating or pre-screening of contaminated samples from bulk food/feed stock.
[Mh] Termos MeSH primário: Aflatoxina B1/toxicidade
Aspergillus flavus/patogenicidade
Zea mays/microbiologia
[Mh] Termos MeSH secundário: Aflatoxina B1/química
Aspergillus flavus/química
Cromatografia em Camada Delgada
Ensaio de Imunoadsorção Enzimática
Índia
Zea mays/parasitologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9N2N2Y55MH (Aflatoxin B1)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180102
[Lr] Data última revisão:
180102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171216
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189760


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[PMID]:28715485
[Au] Autor:Ogunola OF; Hawkins LK; Mylroie E; Kolomiets MV; Borrego E; Tang JD; Williams WP; Warburton ML
[Ad] Endereço:Department of Plant and Soil Sciences, Mississippi State University, Starkville, MS, United States of America.
[Ti] Título:Characterization of the maize lipoxygenase gene family in relation to aflatoxin accumulation resistance.
[So] Source:PLoS One;12(7):e0181265, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Maize (Zea mays L.) is a globally important staple food crop prone to contamination by aflatoxin, a carcinogenic secondary metabolite produced by the fungus Aspergillus flavus. An efficient approach to reduce accumulation of aflatoxin is the development of germplasm resistant to colonization and toxin production by A. flavus. Lipoxygenases (LOXs) are a group of non-heme iron containing dioxygenase enzymes that catalyze oxygenation of polyunsaturated fatty acids (PUFAs). LOX derived oxylipins play critical roles in plant defense against pathogens including A. flavus. The objectives of this study were to summarize sequence diversity and expression patterns for all LOX genes in the maize genome, and map their effect on aflatoxin accumulation via linkage and association mapping. In total, 13 LOX genes were identified, characterized, and mapped. The sequence of one gene, ZmLOX10, is reported from 5 inbred lines. Genes ZmLOX1/2, 5, 8, 9, 10 and 12 (GRMZM2G156861, or V4 numbers ZM00001D042541 and Zm00001D042540, GRMZM2G102760, GRMZM2G104843, GRMZM2G017616, GRMZM2G015419, and GRMZM2G106748, respectively) fell under previously published QTL in one or more mapping populations and are linked to a measurable reduction of aflatoxin in maize grains. Association mapping results found 28 of the 726 SNPs tested were associated with reduced aflatoxin levels at p ≤ 9.71 x 10-4 according to association statistics. These fell within or near nine of the ZmLOX genes. This work confirms the importance of some lipoxygenases for resistance to aflatoxin accumulation and may be used to direct future genetic selection in maize.
[Mh] Termos MeSH primário: Aflatoxinas/metabolismo
Lipoxigenase/genética
Proteínas de Plantas/genética
Zea mays/genética
[Mh] Termos MeSH secundário: Aspergilose/genética
Aspergilose/metabolismo
Aspergillus flavus
Mapeamento Cromossômico
Expressão Gênica
Estudos de Associação Genética
Ligação Genética
Predisposição Genética para Doença
Lipoxigenase/metabolismo
Filogenia
Doenças das Plantas/genética
Doenças das Plantas/microbiologia
Proteínas de Plantas/metabolismo
Polimorfismo de Nucleotídeo Único
Zea mays/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aflatoxins); 0 (Plant Proteins); EC 1.13.11.12 (Lipoxygenase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170718
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0181265


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[PMID]:28693685
[Au] Autor:Taghizadeh-Armaki M; Hedayati MT; Moqarabzadeh V; Ansari S; Mahdavi Omran S; Zarrinfar H; Saber S; Verweij PE; Denning DW; Seyedmousavi S
[Ad] Endereço:1​Invasive Fungi Research Center, Mazandaran University of Medical Sciences, Sari, Iran 2​Department of Medical Mycology and Parasitology, School of Medicine, Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran 3​Department of Medical Mycology and Parasitology, Sc
[Ti] Título:Effect of involved Aspergillus species on galactomannan in bronchoalveolar lavage of patients with invasive aspergillosis.
[So] Source:J Med Microbiol;66(7):898-904, 2017 Jul.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: The detection of galactomannan (GM) in bronchoalveolar lavage (BAL) fluid is an important surrogate marker for the early diagnosis and therapeutic monitoring of invasive aspergillosis (IA), regardless of the involved species of Aspergillus. Here, we utilized the Platelia Aspergillus GM enzyme immunoassay (Bio-Rad) to evaluate the GM index in BAL fluid samples from patients with proven, probable or putative IA due to Aspergillusflavus versus Aspergillusfumigatus. METHODOLOGY: In a prospective study between 2009 and 2015, 116 BAL samples were collected from suspected IA patients referred to two university hospitals in Tehran, Iran. KEY FINDINGS: According to European Organization for Research and Treatment of Cancer and Mycoses Study Group and Blot criteria, 35 patients were classified as IA patients, of which 33 cases tested positive for GM above 0.5 and, among these patients, 22 had a GM index ≥1. Twenty-eight were culture positive for A. flavus and seven for A. fumigatus. The GM index for A. flavus cases was between 0.5-6.5 and those of A. fumigatus ranged from 1 to 6.5. The sensitivity and specificity of a GM index ≥0.5 in cases with A. flavus were 86 and 88 % and for A. fumigatus patients were 100 and 73 %, respectively. CONCLUSION: Overall, the mean GM index in patients with A. fumigatus (3.1) was significantly higher than those of A. flavus (1.6; P-value=0.031) and the sensitivity of GM lower for A. flavus when compared to A. fumigatus. This finding has implications for diagnosis in hospitals and countries with a high proportion of A. flavus infections.
[Mh] Termos MeSH primário: Aspergillus flavus/química
Aspergillus fumigatus/química
Líquido da Lavagem Broncoalveolar/química
Testes Diagnósticos de Rotina/métodos
Aspergilose Pulmonar Invasiva/patologia
Mananas/análise
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Aspergillus flavus/isolamento & purificação
Aspergillus fumigatus/isolamento & purificação
Criança
Ensaio de Imunoadsorção Enzimática/métodos
Feminino
Seres Humanos
Aspergilose Pulmonar Invasiva/diagnóstico
Irã (Geográfico)
Masculino
Meia-Idade
Estudos Prospectivos
Sensibilidade e Especificidade
Adulto Jovem
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Mannans); 11078-30-1 (galactomannan)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170809
[Lr] Data última revisão:
170809
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170712
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000512


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[PMID]:28676272
[Au] Autor:Kauthale S; Tekale S; Damale M; Sangshetti J; Pawar R
[Ad] Endereço:Department of Chemistry, Deogiri College, Station Road, Aurangabad, MS 431 005, India.
[Ti] Título:Synthesis, antioxidant, antifungal, molecular docking and ADMET studies of some thiazolyl hydrazones.
[So] Source:Bioorg Med Chem Lett;27(16):3891-3896, 2017 08 15.
[Is] ISSN:1464-3405
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Some thiazolyl hydrazones were synthesized by one pot reaction of thiophene-2-carbaldehyde or 2, 4-dichlorobenzaldehyde, thiosemicarbazide and various phenacyl bromides which were preliminarily screened for in vitro antioxidant and antifungal activities. Excellent DPPH and H O radical scavenged antioxidant activities were observed with almost all the tested compounds. Compounds 4a, 4b, 4c, 4e, 4f and 4i showed comparable DPPH scavenged antioxidant potential (90.26-96.56%) whereas H O scavenged antioxidant activity (90.98-92.08%) was noticeable in case of 4a and 4f; showing significant antioxidant potential comparable with the standard ascorbic acid (95.3%). In vitro antifungal activity of synthesized compounds against fungal species Candida albicance, Aspergillus niger and Aspergillus flavus was found to be moderate to good as compared with the standard fluconazole and MIC values were found in the range of 3.12-25µg/mL. Molecular docking studies revealed that the compounds 4a, 4b and 4c have a potential to become lead molecules in drug discovery process. In silico ADMET study was also performed for predicting pharmacokinetic and toxicity profile of the synthesized antioxidants which expressed good oral drug like behaviour and non-toxic nature.
[Mh] Termos MeSH primário: Antifúngicos/farmacologia
Antioxidantes/farmacologia
Simulação por Computador
Hidrazonas/farmacologia
[Mh] Termos MeSH secundário: Antifúngicos/síntese química
Antifúngicos/química
Antioxidantes/síntese química
Antioxidantes/química
Aspergillus flavus/efeitos dos fármacos
Aspergillus niger/efeitos dos fármacos
Candida/efeitos dos fármacos
Relação Dose-Resposta a Droga
Hidrazonas/síntese química
Hidrazonas/química
Testes de Sensibilidade Microbiana
Estrutura Molecular
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Antioxidants); 0 (Hydrazones)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171125
[Lr] Data última revisão:
171125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170706
[St] Status:MEDLINE


  6 / 2280 MEDLINE  
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[PMID]:28658268
[Au] Autor:Wu MY; Mead ME; Kim SC; Rokas A; Yu JH
[Ad] Endereço:Departments of Bacteriology and Genetics, University of Wisconsin, Madison, Wisconsin, United States of America.
[Ti] Título:WetA bridges cellular and chemical development in Aspergillus flavus.
[So] Source:PLoS One;12(6):e0179571, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bridging cellular reproduction and survival is essential for all life forms. Aspergillus fungi primarily reproduce by forming asexual spores called conidia, whose formation and maturation is governed by the central genetic regulatory circuit BrlA→AbaA→WetA. Here, we report that WetA is a multi-functional regulator that couples spore differentiation and survival, and governs proper chemical development in Aspergillus flavus. The deletion of wetA results in the formation of conidia with defective cell walls and no intra-cellular trehalose, leading to reduced stress tolerance, a rapid loss of viability, and disintegration of spores. WetA is also required for normal vegetative growth, hyphal branching, and production of aflatoxins. Targeted and genome-wide expression analyses reveal that WetA exerts feedback control of brlA and that 5,700 genes show altered mRNA levels in the mutant conidia. Functional category analyses of differentially expressed genes in ΔwetA RNA-seq data indicate that WetA contributes to spore integrity and maturity by properly regulating the metabolic pathways of trehalose, chitin, α-(1,3)-glucan, ß-(1,3)-glucan, melanin, hydrophobins, and secondary metabolism more generally. Moreover, 160 genes predicted to encode transcription factors are differentially expressed by the absence of wetA, suggesting that WetA may play a global regulatory role in conidial development. Collectively, we present a comprehensive model for developmental control that bridges spore differentiation and survival in A. flavus.
[Mh] Termos MeSH primário: Aspergillus flavus/metabolismo
Proteínas Fúngicas/metabolismo
Regulação Fúngica da Expressão Gênica
Reprodução Assexuada
[Mh] Termos MeSH secundário: Aspergillus flavus/genética
Sobrevivência Celular
Proteínas Fúngicas/genética
Genes Fúngicos
Hifas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungal Proteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170629
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179571


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[PMID]:28648291
[Au] Autor:Peromingo B; Rodríguez M; Delgado J; Andrade MJ; Rodríguez A
[Ad] Endereço:Food Hygiene and Safety, Meat and Meat Products Research Institute, Faculty of Veterinary Science, University of Extremadura, Avda. de las Ciencias, s/n, 10003 Cáceres, Spain.
[Ti] Título:Gene expression as a good indicator of aflatoxin contamination in dry-cured ham.
[So] Source:Food Microbiol;67:31-40, 2017 Oct.
[Is] ISSN:1095-9998
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Aspergillus flavus and Aspergillus parasiticus are mould species producers of aflatoxins (AFs) and may grow on dry-cured ham during the ripening process. In this study, the influence of different water activity (a ) and temperatures on the temporal relative expression of three genes involved in AFs biosynthesis and their relationship with AFs production on dry-cured ham-based medium were evaluated. In general, the regulatory aflR and aflS genes showed similar expression patterns, and the expression of the structural aflP gene was much higher than that obtained for aflR and aflS genes. Regarding A. flavus, a decrease of a regardless of temperature caused an increase of the expression of the regulatory aflR and aflS genes. Concerning A. parasiticus, the highest and lowest expression values of the regulatory aflR and aflS genes were found at 0.95 a and 0.85 a , respectively. The expression of the structural aflP gene of both species was stimulated at low temperature and a . The PCA analysis indicated that both toxigenic species showed a strong correlation between the relative expression of the aflR and aflS genes and the concentration of AFs under conditions which simulate dry-cured ham ripening. This suggests that an early detection of the expression of regulatory genes can be a good indicator of possible AFs contamination of dry-cured ham through ripening.
[Mh] Termos MeSH primário: Aflatoxinas/análise
Aspergillus flavus/metabolismo
Aspergillus/metabolismo
Contaminação de Alimentos/análise
Proteínas Fúngicas/genética
Produtos da Carne/microbiologia
[Mh] Termos MeSH secundário: Aflatoxinas/metabolismo
Animais
Aspergillus/genética
Aspergillus/isolamento & purificação
Aspergillus flavus/genética
Aspergillus flavus/isolamento & purificação
Manipulação de Alimentos
Proteínas Fúngicas/metabolismo
Regulação Fúngica da Expressão Gênica
Produtos da Carne/análise
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aflatoxins); 0 (Fungal Proteins)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170822
[Lr] Data última revisão:
170822
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170627
[St] Status:MEDLINE


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[PMID]:28640833
[Au] Autor:Yang X; Zhang Q; Chen ZY; Liu H; Li P
[Ad] Endereço:Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing, P. R. China.
[Ti] Título:Investigation of Pseudomonas fluorescens strain 3JW1 on preventing and reducing aflatoxin contaminations in peanuts.
[So] Source:PLoS One;12(6):e0178810, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Pseudomonas fluorescens strain 3JW1, which has a broad-spectrum antimicrobial activity, was studied to investigate whether it affects the amounts of aflatoxin B1 (AFB1) produced by Aspergillus flavus. It was found that the bacterium reduced the amounts of AFB1 in potato dextrose broth (PDB) and peanut medium by 97.8% and 99.4%, respectively. It also reduced AFB1 by ~183 µg/kg (55.8%) when applied onto peanut kernels. This strain reduced AFB1 via three mechanisms. First, it significantly inhibited A. flavus growth; second, our data showed that strain 3JW1 inhibits aflatoxin biosynthesis by A. flavus; and third, P. fluorescens strain 3JW1 is capable of degrading AFB1 at a rate as high as 88.3% in 96 hours. This is the first report demonstrating that Pseudomonas fluorescens can reduce toxin contamination caused by A. flavus on peanut kernels. Our findings indicate that P. fluorescens strain 3JW1 had multiple effects including reducing A. flavus infection and aflatoxin contamination. And the results also highlight the potential applications of the strain 3JW1 for the biological control of aflatoxin contamination in peanuts and other susceptible crops.
[Mh] Termos MeSH primário: Aflatoxina B1/análise
Arachis/microbiologia
Contaminação de Alimentos/prevenção & controle
Pseudomonas fluorescens/fisiologia
[Mh] Termos MeSH secundário: Aflatoxina B1/biossíntese
Aspergillus flavus/metabolismo
Aspergillus flavus/fisiologia
Contaminação de Alimentos/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9N2N2Y55MH (Aflatoxin B1)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170623
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0178810


  9 / 2280 MEDLINE  
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[PMID]:28582664
[Au] Autor:Medina A; Gilbert MK; Mack BM; OBrian GR; Rodríguez A; Bhatnagar D; Payne G; Magan N
[Ad] Endereço:Applied Mycology Group, Cranfield Soil and AgriFood Institute, Cranfield University, Beds MK43 0AL, UK.
[Ti] Título:Interactions between water activity and temperature on the Aspergillus flavus transcriptome and aflatoxin B production.
[So] Source:Int J Food Microbiol;256:36-44, 2017 Sep 01.
[Is] ISSN:1879-3460
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Effects of Aspergillus flavus colonization of maize kernels under different water activities (a ; 0.99 and 0.91) and temperatures (30, 37°C) on (a) aflatoxin B (AFB ) production and (b) the transcriptome using RNAseq were examined. There was no significant difference (p=0.05) in AFB production at 30 and 37°C and 0.99 a . However, there was a significant (p=0.05) increase in AFB at 0.91 a at 37°C when compared with 30°C/0.99 a . Environmental stress effects using gene ontology enrichment analysis of the RNA-seq results for increasing temperature at 0.99 and 0.91 a showed differential expression of 2224 and 481 genes, respectively. With decreasing water availability, 4307 were affected at 30°C and 702 genes at 37°C. Increasing temperature from 30 to 37°C at both a levels resulted in 12 biological processes being upregulated and 9 significantly downregulated. Decreasing a at both temperatures resulted in 22 biological processes significantly upregulated and 25 downregulated. The interacting environmental factors influenced functioning of the secondary metabolite gene clusters for aflatoxins and cyclopiazonic acid (CPA). An elevated number of genes were co-regulated by both a and temperature. An interaction effect for 4 of the 25 AFB genes, including regulatory and transcription activators occurred. For CPA, all 5 biosynthetic genes were affected by a stress, regardless of temperature. The molecular regulation of A. flavus in maize is discussed.
[Mh] Termos MeSH primário: Aflatoxina B1/biossíntese
Aspergillus flavus/genética
Aspergillus flavus/metabolismo
Água/metabolismo
Zea mays/microbiologia
[Mh] Termos MeSH secundário: Sequência de Bases
Meio Ambiente
Indóis/metabolismo
Análise de Sequência de RNA
Temperatura Ambiente
Transcriptoma/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Indoles); 059QF0KO0R (Water); 9N2N2Y55MH (Aflatoxin B1); X9TLY4580Z (cyclopiazonic acid)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170606
[St] Status:MEDLINE


  10 / 2280 MEDLINE  
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[PMID]:28582408
[Au] Autor:Lv Y
[Ad] Endereço:College of Biological Engineering, Henan University of Technology, Zhengzhou, China.
[Ti] Título:Proteome-wide profiling of protein lysine acetylation in Aspergillus flavus.
[So] Source:PLoS One;12(6):e0178603, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Protein lysine acetylation is a prevalent post-translational modification that plays pivotal roles in various biological processes in both prokaryotes and eukaryotes. Aspergillus flavus, as an aflatoxin-producing fungus, has attracted tremendous attention due to its health impact on agricultural commodities. Here, we performed the first lysine-acetylome mapping in this filamentous fungus using immune-affinity-based purification integrated with high-resolution mass spectrometry. Overall, we identified 1383 lysine-acetylation sites in 652 acetylated proteins, which account for 5.18% of the total proteins in A. flavus. According to bioinformatics analysis, the acetylated proteins are involved in various cellular processes involving the ribosome, carbon metabolism, antibiotic biosynthesis, secondary metabolites, and the citrate cycle and are distributed in diverse subcellular locations. Additionally, we demonstrated for the first time the acetylation of fatty acid synthase α and ß encoded by aflA and aflB involved in the aflatoxin-biosynthesis pathway (cluster 54), as well as backbone enzymes from secondary metabolite clusters 20 and 21 encoded by AFLA_062860 and AFLA_064240, suggesting important roles for acetylation associated with these processes. Our findings illustrating abundant lysine acetylation in A. flavus expand our understanding of the fungal acetylome and provided insight into the regulatory roles of acetylation in secondary metabolism.
[Mh] Termos MeSH primário: Aspergillus flavus/genética
Proteínas Fúngicas/genética
Genoma Fúngico
Lisina/metabolismo
Processamento de Proteína Pós-Traducional
Proteoma/genética
[Mh] Termos MeSH secundário: Acetilação
Aflatoxinas/biossíntese
Aflatoxinas/genética
Sequência de Aminoácidos
Aspergillus flavus/metabolismo
Mapeamento Cromossômico
Proteínas Fúngicas/metabolismo
Perfilação da Expressão Gênica
Ontologia Genética
Anotação de Sequência Molecular
Proteoma/metabolismo
Metabolismo Secundário/genética
Alinhamento de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aflatoxins); 0 (Fungal Proteins); 0 (Proteome); K3Z4F929H6 (Lysine)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170606
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0178603



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