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Pesquisa : B01.300.381.081.420 [Categoria DeCS]
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  1 / 3351 MEDLINE  
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[PMID]:28747316
[Au] Autor:Suresh S; Markossian S; Osmani AH; Osmani SA
[Ad] Endereço:Department of Molecular Genetics, The Ohio State University, Columbus, OH.
[Ti] Título:Mitotic nuclear pore complex segregation involves Nup2 in .
[So] Source:J Cell Biol;216(9):2813-2826, 2017 Sep 04.
[Is] ISSN:1540-8140
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Transport through nuclear pore complexes (NPCs) during interphase is facilitated by the nucleoporin Nup2 via its importin α- and Ran-binding domains. However, and vertebrate Nup2 also locate to chromatin during mitosis, suggestive of mitotic functions. In this study, we report that Nup2 is required for mitotic NPC inheritance in Interestingly, the role of Nup2 during mitotic NPC segregation is independent of its importin α- and Ran-binding domains but relies on a central targeting domain that is necessary for localization and viability. To test whether mitotic chromatin-associated Nup2 might function to bridge NPCs with chromatin during segregation, we provided an artificial link between NPCs and chromatin via Nup133 and histone H1. Using this approach, we bypassed the requirement of Nup2 for NPC segregation. This indicates that cells ensure accurate mitotic NPC segregation to daughter nuclei by linking mitotic DNA and NPC segregation via the mitotic specific chromatin association of Nup2.
[Mh] Termos MeSH primário: Aspergillus nidulans/metabolismo
Proteínas Fúngicas/metabolismo
Mitose
Complexo de Proteínas Formadoras de Poros Nucleares/metabolismo
Poro Nuclear/metabolismo
[Mh] Termos MeSH secundário: Aspergillus nidulans/genética
Aspergillus nidulans/crescimento & desenvolvimento
Cromatina/genética
Cromatina/metabolismo
DNA Fúngico/genética
DNA Fúngico/metabolismo
Proteínas Fúngicas/genética
Histonas/metabolismo
Microscopia de Fluorescência
Mutação
Poro Nuclear/genética
Complexo de Proteínas Formadoras de Poros Nucleares/genética
Transdução de Sinais
Fatores de Tempo
Imagem com Lapso de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chromatin); 0 (DNA, Fungal); 0 (Fungal Proteins); 0 (Histones); 0 (Nuclear Pore Complex Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170728
[St] Status:MEDLINE
[do] DOI:10.1083/jcb.201610019


  2 / 3351 MEDLINE  
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[PMID]:29367149
[Au] Autor:Shanuja SK; Iswarya S; Gnanamani A
[Ad] Endereço:Microbiology Division, CSIR-CLRI, Adyar, Chennai 20, India.
[Ti] Título:Marine fungal DHICA as a UVB protectant: Assessment under in vitro and in vivo conditions.
[So] Source:J Photochem Photobiol B;179:139-148, 2018 Feb.
[Is] ISSN:1873-2682
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The present study explores UVB protective role of a melanin precursor namely DHICA (5,6- Dihydroxyindole-2-carboxylic acid) expressed by the marine imperfect fungus Aspergillus nidulans. In brief, A. nidulans grown in a modified growth medium for the period of 5 days at 25 °C under shaking conditions and the extracellular medium free from fungal biomass used for the extraction of DHICA. The extracted DHICA further exposed to partial purification and subjected to UVB protection studies using HaCaT cells and Balb/c mice independently. DHICA obtained in the present study found soluble in water. Experiments on HaCaT cell compatibility revealed nil cell death up to 500 µM concentration of DHICA. UVB protection studies under in vitro conditions emphasizes DHICA significantly protect HaCaT cells from UVB exposure by quenching the generated ROS, reducing cell apoptosis, maintain the cellular integrity and sequentially down regulating the LPO (Lipid peroxidation) and up-regulating the antioxidant enzyme (SOD (Superoxide Dismutase), Catalase, GPx (Glutathione peroxidase)) respectively. Further, experiments on cell cycle arrest analysis, gelatin zymography, and western blot analysis on COX-2 and TNF-alpha, IHC (Immunohistochemistry) on apoptotic markers (Bax, Bcl2) substantiate the protective role of DHICA. Furthermore, in vivo studies on BALB/c mice carried out and compared with the sunscreen cream with sun protective factor (SPF) of 20. Analysis of skin sections of experimental samples revealed that an appreciable reduction in the epidermal thickness of the skin samples of mice pre-exposed to DHICA followed by UVB exposure compared to UVB exposure alone. RT-PCR results on various inflammatory apoptotic markers also suggested that DHICA has UVB protective potential. The observations made in the present study explore the possible application of DHICA alone as a sun-protective agent for skin care.
[Mh] Termos MeSH primário: Apoptose/efeitos dos fármacos
Aspergillus nidulans/metabolismo
Indóis/farmacologia
Pele/efeitos dos fármacos
Raios Ultravioleta
[Mh] Termos MeSH secundário: Animais
Antioxidantes/metabolismo
Catalase/metabolismo
Linhagem Celular
Dano ao DNA/efeitos dos fármacos
Feminino
Glutationa Peroxidase/metabolismo
Seres Humanos
Indóis/química
Peroxidação de Lipídeos/efeitos dos fármacos
Melaninas/metabolismo
Camundongos
Camundongos Endogâmicos BALB C
Estresse Oxidativo/efeitos dos fármacos
Substâncias Protetoras/química
Substâncias Protetoras/farmacologia
Espécies Reativas de Oxigênio/metabolismo
Pele/patologia
Pele/efeitos da radiação
Superóxido Dismutase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Indoles); 0 (Melanins); 0 (Protective Agents); 0 (Reactive Oxygen Species); 4790-08-3 (5,6-dihydroxy-2-indolylcarboxylic acid); EC 1.11.1.6 (Catalase); EC 1.11.1.9 (Glutathione Peroxidase); EC 1.15.1.1 (Superoxide Dismutase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180223
[Lr] Data última revisão:
180223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180126
[St] Status:MEDLINE


  3 / 3351 MEDLINE  
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[PMID]:29293643
[Au] Autor:Regulin A; Kempken F
[Ad] Endereço:Botanisches Institut und Botanischer Garten, Christian-Albrechts-Universität, Kiel, Germany.
[Ti] Título:Fungal genotype determines survival of Drosophila melanogaster when competing with Aspergillus nidulans.
[So] Source:PLoS One;13(1):e0190543, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Fungi produce an astonishing variety of secondary metabolites, some of which belong to the most toxic compounds in the living world. Several fungal metabolites have anti-insecticidal properties which may yield advantages to the fungus in competition with insects for exploitation of environmental resources. Using the Drosophila melanogaster/Aspergillus nidulans ecological model system to assess secondary metabolite mutant genotypes, we find a major role for the veA allele in insect/fungal confrontations that exceeds the influence of other factors such as LaeA. VeA along with LaeA is a member of a transcriptional complex governing secondary metabolism in A. nidulans. However, historically a mutant veA allele, veA1 reduced in secondary metabolite output, has been used in many studies of this model organism. To test the significance of this allele in our system, Aspergillus nidulans veA wild type, veA1, ΔveA and ΔlaeA were evaluated in confrontation assays to analyze egg laying activity, and the survival rate of larvae. The veA1 genetic background led to a significant increase of larval survival. Adult flies were observed almost exclusively on veA1, ΔveA or ΔlaeA genetic backgrounds, suggesting a role for the velvet complex in insect/fungal interactions. This effect was most profound using the veA1 mutant. Hence, larval survival in confrontations is highly affected by the fungal genotype.
[Mh] Termos MeSH primário: Aspergillus nidulans/genética
Drosophila melanogaster/fisiologia
[Mh] Termos MeSH secundário: Animais
Aspergillus nidulans/crescimento & desenvolvimento
Drosophila melanogaster/embriologia
Drosophila melanogaster/crescimento & desenvolvimento
Genótipo
Interações Hospedeiro-Patógeno
Larva
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180103
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190543


  4 / 3351 MEDLINE  
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[PMID]:27775185
[Au] Autor:Oakley CE; Ahuja M; Sun WW; Entwistle R; Akashi T; Yaegashi J; Guo CJ; Cerqueira GC; Russo Wortman J; Wang CC; Chiang YM; Oakley BR
[Ad] Endereço:Department of Molecular Biosciences, University of Kansas, 1200 Sunnyside Avenue, Lawrence, Kansas, 66045, USA.
[Ti] Título:Discovery of McrA, a master regulator of Aspergillus secondary metabolism.
[So] Source:Mol Microbiol;103(2):347-365, 2017 Jan.
[Is] ISSN:1365-2958
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Fungal secondary metabolites (SMs) are extremely important in medicine and agriculture, but regulation of their biosynthesis is incompletely understood. We have developed a genetic screen in Aspergillus nidulans for negative regulators of fungal SM gene clusters and we have used this screen to isolate mutations that upregulate transcription of the non-ribosomal peptide synthetase gene required for nidulanin A biosynthesis. Several of these mutations are allelic and we have identified the mutant gene by genome sequencing. The gene, which we designate mcrA, is conserved but uncharacterized, and it encodes a putative transcription factor. Metabolite profiles of mcrA deletant, mcrA overexpressing, and parental strains reveal that mcrA regulates at least ten SM gene clusters. Deletion of mcrA stimulates SM production even in strains carrying a deletion of the SM regulator laeA, and deletion of mcrA homologs in Aspergillus terreus and Penicillum canescens alters the secondary metabolite profile of these organisms. Deleting mcrA in a genetic dereplication strain has allowed us to discover two novel compounds as well as an antibiotic not known to be produced by A. nidulans. Deletion of mcrA upregulates transcription of hundreds of genes including many that are involved in secondary metabolism, while downregulating a smaller number of genes.
[Mh] Termos MeSH primário: Aspergillus nidulans/genética
Aspergillus nidulans/metabolismo
Peptídeo Sintases/genética
Peptídeo Sintases/metabolismo
[Mh] Termos MeSH secundário: Proteínas Fúngicas/genética
Proteínas Fúngicas/metabolismo
Regulação Fúngica da Expressão Gênica
Genes Fúngicos
Família Multigênica
Mutação
Metabolismo Secundário
Deleção de Sequência
Fatores de Transcrição/genética
Fatores de Transcrição/metabolismo
Ativação Transcricional
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungal Proteins); 0 (Transcription Factors); EC 6.3.2.- (Peptide Synthases)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:180101
[Lr] Data última revisão:
180101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE
[do] DOI:10.1111/mmi.13562


  5 / 3351 MEDLINE  
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[PMID]:28465348
[Au] Autor:Itoh E; Odakura R; Oinuma KI; Shimizu M; Masuo S; Takaya N
[Ad] Endereço:From the Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan.
[Ti] Título:Sirtuin E is a fungal global transcriptional regulator that determines the transition from the primary growth to the stationary phase.
[So] Source:J Biol Chem;292(26):11043-11054, 2017 06 30.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In response to limited nutrients, fungal cells exit the primary growth phase, enter the stationary phase, and cease proliferation. Although fundamental to microbial physiology in many environments, the regulation of this transition is poorly understood but likely involves many transcriptional regulators. These may include the sirtuins, which deacetylate acetyllysine residues of histones and epigenetically regulate global transcription. Therefore, we investigated the role of a nuclear sirtuin, sirtuin E (SirE), from the ascomycete fungus An strain with a disrupted gene (SirEΔ) accumulated more acetylated histone H3 during the stationary growth phase when was expressed at increased levels in the wild type. SirEΔ exhibited decreased mycelial autolysis, conidiophore development, sterigmatocystin biosynthesis, and production of extracellular hydrolases. Moreover, the transcription of the genes involved in these processes was also decreased, indicating that SirE is a histone deacetylase that up-regulates these activities in the stationary growth phase. Transcriptome analyses indicated that SirE repressed primary carbon and nitrogen metabolism and cell-wall synthesis. Chromatin immunoprecipitation demonstrated that SirE deacetylates acetylated Lys-9 residues in histone H3 at the gene promoters of α-1,3-glucan synthase ( ), glycolytic phosphofructokinase ( ), and glyceraldehyde 3-phosphate ( ), indicating that SirE represses the expression of these primary metabolic genes. In summary, these results indicate that SirE facilitates the metabolic transition from the primary growth phase to the stationary phase. Because the observed gene expression profiles in stationary phase matched those resulting from carbon starvation, SirE appears to control this metabolic transition via a mechanism associated with the starvation response.
[Mh] Termos MeSH primário: Aspergillus nidulans/metabolismo
Proteínas Fúngicas/metabolismo
Regulação Fúngica da Expressão Gênica/fisiologia
Sirtuínas/metabolismo
Fatores de Transcrição/metabolismo
Transcriptoma/fisiologia
[Mh] Termos MeSH secundário: Aspergillus nidulans/genética
Proteínas Fúngicas/genética
Sirtuínas/genética
Fatores de Transcrição/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Fungal Proteins); 0 (Transcription Factors); EC 3.5.1.- (Sirtuins)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171229
[Lr] Data última revisão:
171229
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M116.753772


  6 / 3351 MEDLINE  
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[PMID]:29194456
[Au] Autor:Suresh S; Abdurehman L; Osmani AH; Osmani SA
[Ad] Endereço:Department of Molecular Genetics, The Ohio State University, Columbus, Ohio, United States of America.
[Ti] Título:Tools for retargeting proteins within Aspergillus nidulans.
[So] Source:PLoS One;12(12):e0189077, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Endogenously tagging proteins with green fluorescent protein (GFP) enables the visualization of the tagged protein using live cell microscopy. GFP-tagging is widely utilized to study biological processes in model experimental organisms including filamentous fungi such as Aspergillus nidulans. Many strains of A. nidulans have therefore been generated with different proteins endogenously tagged with GFP. To further enhance experimental approaches based upon GFP-tagging, we have adapted the GFP Binding Protein (GBP) system for A. nidulans. GBP is a genetically encoded Llama single chain antibody against GFP which binds GFP with high affinity. Using gene replacement approaches, it is therefore possible to link GBP to anchor proteins, which will then retarget GFP-tagged proteins away from their normal location to the location of the anchor-GBP protein. To facilitate this approach in A. nidulans, we made four base plasmid cassettes that can be used to generate gene replacement GBP-tagging constructs by utilizing fusion PCR. Using these base cassettes, fusion PCR, and gene targeting approaches, we generated strains with SPA10-GBP and Tom20-GBP gene replacements. These strains enabled test targeting of GFP-tagged proteins to septa or to the surface of mitochondria respectively. SPA10-GBP is shown to effectively target GFP-tagged proteins to both forming and mature septa. Tom20-GBP has a higher capacity to retarget GFP-tagged proteins being able to relocate all Nup49-GFP from its location within nuclear pore complexes (NPCs) to the cytoplasm in association with mitochondria. Notably, removal of Nup49-GFP from NPCs causes cold sensitivity as does deletion of the nup49 gene. The cassette constructs described facilitate experimental approaches to generate precise protein-protein linkages in fungi. The A. nidulans SPA10-GBP and Tom20-GBP strains can be utilized to modulate other GFP-tagged proteins of interest.
[Mh] Termos MeSH primário: Aspergillus nidulans/metabolismo
Proteínas Fúngicas/metabolismo
[Mh] Termos MeSH secundário: Proteínas de Fluorescência Verde/metabolismo
Mitocôndrias/metabolismo
Reação em Cadeia da Polimerase
Transporte Proteico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungal Proteins); 147336-22-9 (Green Fluorescent Proteins)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171202
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189077


  7 / 3351 MEDLINE  
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[PMID]:28746736
[Au] Autor:Trienens M; Kraaijeveld K; Wertheim B
[Ad] Endereço:Groningen Institute for Evolutionary Life Sciences, University of Groningen, Groningen, The Netherlands.
[Ti] Título:Defensive repertoire of Drosophila larvae in response to toxic fungi.
[So] Source:Mol Ecol;26(19):5043-5057, 2017 Oct.
[Is] ISSN:1365-294X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Chemical warfare including insecticidal secondary metabolites is a well-known strategy for environmental microbes to monopolize a food source. Insects in turn have evolved behavioural and physiological defences to eradicate or neutralize the harmful microorganisms. We studied the defensive repertoire of insects in this interference competition by combining behavioural and developmental assays with whole-transcriptome time-series analysis. Confrontation with the toxic filamentous fungus Aspergillus nidulans severely reduced the survival of Drosophila melanogaster larvae. Nonetheless, the larvae did not behaviourally avoid the fungus, but aggregated at it. Confrontation with fungi strongly affected larval gene expression, including many genes involved in detoxification (e.g., CYP, GST and UGT genes) and the formation of the insect cuticle (e.g., Tweedle genes). The most strongly upregulated genes were several members of the insect-specific gene family Osiris, and CHK-kinase-like domains were over-represented. Immune responses were not activated, reflecting the competitive rather than pathogenic nature of the antagonistic interaction. While internal microbes are widely acknowledged as important, our study emphasizes the underappreciated role of environmental microbes as fierce competitors.
[Mh] Termos MeSH primário: Drosophila melanogaster/genética
Interações Hospedeiro-Patógeno/genética
[Mh] Termos MeSH secundário: Animais
Aspergillus nidulans
Drosophila melanogaster/microbiologia
Genes de Insetos
Larva/genética
Larva/microbiologia
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171220
[Lr] Data última revisão:
171220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE
[do] DOI:10.1111/mec.14254


  8 / 3351 MEDLINE  
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[PMID]:28738504
[Au] Autor:Pardo-Planas O; Prade RA; Müller M; Atiyeh HK; Wilkins MR
[Ad] Endereço:Department of Biosystems and Agricultural Engineering, 111 Agriculture Hall, Oklahoma State University, Stillwater, OK 74078, USA.
[Ti] Título:Prevention of melanin formation during aryl alcohol oxidase production under growth-limited conditions using an Aspergillus nidulans cell factory.
[So] Source:Bioresour Technol;243:874-882, 2017 Nov.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:An Aspergillus nidulans cell factory was genetically engineered to produce an aryl alcohol oxidase (AAO). The cell factory initiated production of melanin when growth-limited conditions were established using stationary plates and shaken flasks. This phenomenon was more pronounced when the strain was cultured in a trickle bed reactor (TBR). This study investigated different approaches to reduce melanin formation in fungal mycelia and liquid medium in order to increase the enzyme production yield. Removal of copper from the medium recipe reduced melanin formation in agar cultures and increased enzyme activities by 48% in agitated liquid cultures. Copper has been reported as a key element for tyrosinase, an enzyme responsible for melanin production. Ascorbic acid (0.44g/L) stopped melanin accumulation, did not affect growth parameters and resulted in AAO activity that was more than two-fold greater than a control treatment with no ascorbic acid.
[Mh] Termos MeSH primário: Oxirredutases do Álcool/metabolismo
Aspergillus nidulans
Melaninas
[Mh] Termos MeSH secundário: Ácido Ascórbico
Reatores Biológicos
Monofenol Mono-Oxigenase
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Melanins); EC 1.1.- (Alcohol Oxidoreductases); EC 1.1.3.13 (alcohol oxidase); EC 1.14.18.1 (Monophenol Monooxygenase); PQ6CK8PD0R (Ascorbic Acid)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170726
[St] Status:MEDLINE


  9 / 3351 MEDLINE  
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[PMID]:28595329
[Au] Autor:Fekete E; Flipphi M; Ág N; Kavalecz N; Cerqueira G; Scazzocchio C; Karaffa L
[Ad] Endereço:Department of Biochemical Engineering, University of Debrecen, 4032, Hungary.
[Ti] Título:A mechanism for a single nucleotide intron shift.
[So] Source:Nucleic Acids Res;45(15):9085-9092, 2017 Sep 06.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Spliceosomal introns can occupy nearby rather than identical positions in orthologous genes (intron sliding or shifting). Stwintrons are complex intervening sequences, where an 'internal' intron interrupts one of the sequences essential for splicing, generating after its excision, a newly formed canonical intron defined as 'external'. In one experimentally demonstrated configuration, two alternatively excised internal introns, overlapping by one G, disrupt respectively the donor and the acceptor sequence of an external intron, leading to mRNAs encoding identical proteins. In a gene encoding a DHA1 antiporter in Pezizomycotina, we find a variety of predicted intron configurations interrupting the DNA stretch encoding a conserved peptidic sequence. Some sport a stwintron where the internal intron interrupts the donor of the external intron (experimentally confirmed for Aspergillus nidulans). In others, we found and demonstrate (for Trichoderma reesei) alternative, overlapping internal introns. Discordant canonical introns, one nt apart, are present in yet other species, exactly as predicted by the alternative loss of either of the internal introns at the DNA level from an alternatively spliced stwintron. An evolutionary pathway of 1 nt intron shift, involving an alternatively spliced stwintron intermediate is proposed on the basis of the experimental and genomic data presented.
[Mh] Termos MeSH primário: Processamento Alternativo
Genoma Fúngico
Íntrons
Nucleotídeos/genética
Filogenia
RNA Mensageiro/genética
[Mh] Termos MeSH secundário: Ascomicetos/classificação
Ascomicetos/genética
Ascomicetos/metabolismo
Aspergillus nidulans/classificação
Aspergillus nidulans/genética
Aspergillus nidulans/metabolismo
Sequência de Bases
Sequência Conservada
DNA Fúngico/genética
DNA Fúngico/metabolismo
Proteínas Fúngicas/genética
Proteínas Fúngicas/metabolismo
Nucleotídeos/metabolismo
RNA Mensageiro/metabolismo
Alinhamento de Sequência
Homologia de Sequência do Ácido Nucleico
Spliceossomos/genética
Spliceossomos/metabolismo
Trichoderma/classificação
Trichoderma/genética
Trichoderma/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Fungal); 0 (Fungal Proteins); 0 (Nucleotides); 0 (RNA, Messenger)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170609
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkx520


  10 / 3351 MEDLINE  
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[PMID]:28481894
[Au] Autor:Park HS; Lee MK; Kim SC; Yu JH
[Ad] Endereço:School of Food Science and Biotechnology, Institute of Agricultural Science and Technology, Kyungpook National University, Daegu, Republic of Korea.
[Ti] Título:The role of VosA/VelB-activated developmental gene vadA in Aspergillus nidulans.
[So] Source:PLoS One;12(5):e0177099, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The filamentous fungus Aspergillus nidulans primarily reproduces by forming asexual spores called conidia, the integrity of which is governed by the NF-κB type velvet regulators VosA and VelB. The VosA-VelB hetero-complex regulates the expression of spore-specific structural and regulatory genes during conidiogenesis. Here, we characterize one of the VosA/VelB-activated developmental genes, called vadA, the expression of which in conidia requires activity of both VosA and VelB. VadA (AN5709) is predicted to be a 532-amino acid length fungal-specific protein with a highly conserved domain of unknown function (DUF) at the N-terminus. This DUF was found to be conserved in many Ascomycota and some Glomeromycota species, suggesting a potential evolutionarily conserved function of this domain in fungi. Deletion studies of vadA indicate that VadA is required for proper downregulation of brlA, fksA, and rodA, and for proper expression of tpsA and orlA during sporogenesis. Moreover, vadA null mutant conidia exhibit decreased trehalose content, but increased ß(1,3)-glucan levels, lower viability, and reduced tolerance to oxidative stress. We further demonstrate that the vadA null mutant shows increased production of the mycotoxin sterigmatocystin. In summary, VadA is a dual-function novel regulator that controls development and secondary metabolism, and participates in bridging differentiation and viability of newly formed conidia in A. nidulans.
[Mh] Termos MeSH primário: Aspergillus nidulans/genética
Regulação da Expressão Gênica no Desenvolvimento
Regulação Fúngica da Expressão Gênica
Genes Fúngicos
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Proteínas Fúngicas/química
Proteínas Fúngicas/genética
Filogenia
Homologia de Sequência de Aminoácidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungal Proteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170509
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0177099



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