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[PMID]:28409126
[Au] Autor:Yang Y; Ye Q; Li K; Li Z; Bo X; Li Z; Xu Y; Wang S; Wang P; Chen H; Wang J
[Ad] Endereço:Academy of Military Medical SciencesBeijing, China.
[Ti] Título:Genomics and Comparative Genomic Analyses Provide Insight into the Taxonomy and Pathogenic Potential of Novel Pathogens.
[So] Source:Front Cell Infect Microbiol;7:105, 2017.
[Is] ISSN:2235-2988
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Over the last 50 years, newly described species of -like fungi have been implicated globally as sources of systemic human mycosis (emmonsiosis). Their ability to convert into yeast-like cells capable of replication and extra-pulmonary dissemination during the course of infection differentiates them from classical species. Immunocompromised patients are at highest risk of emmonsiosis and exhibit high mortality rates. In order to investigate the molecular basis for pathogenicity of the newly described species, genomic sequencing and comparative genomic analyses of sp. 5z489, which was isolated from a non-deliberately immunosuppressed diabetic patient in China and represents a novel seventh isolate of -like fungi, was performed. The genome size of 5z489 was 35.5 Mbp in length, which is ~5 Mbp larger than other strains. Further, 9,188 protein genes were predicted in the 5z489 genome and 16% of the assembly was identified as repetitive elements, which is the largest abundance in species. Phylogenetic analyses based on whole genome data classified 5z489 and CAC-2015a, another novel isolate, as members of the genus . Our analyses showed that divergences among occurred much earlier than other genera within the family Ajellomycetaceae, suggesting relatively distant evolutionary relationships among the genus. Through comparisons of species, we discovered significant pathogenicity characteristics within the genus as well as putative virulence factors that may play a role in the infection and pathogenicity of the novel strains. Moreover, our analyses revealed a novel distribution mode of DNA methylation patterns across the genome of 5z489, with >50% of methylated bases located in intergenic regions. These methylation patterns differ considerably from other reported fungi, where most methylation occurs in repetitive loci. It is unclear if this difference is related to physiological adaptations of new , but this question warrants further investigation. Overall, our analyses provide a framework from which to further study the evolutionary dynamics of strains and identity the underlying molecular mechanisms that determine the infectious and pathogenic potency of these fungal pathogens, and also provide insight into potential targets for therapeutic intervention of emmonsiosis and further research.
[Mh] Termos MeSH primário: Chrysosporium/genética
Chrysosporium/patogenicidade
Genômica
[Mh] Termos MeSH secundário: China
Biologia Computacional
DNA Fúngico/química
DNA Fúngico/genética
Complicações do Diabetes
Genoma Fúngico
Seres Humanos
Micoses/microbiologia
Filogenia
Análise de Sequência de DNA
Fatores de Virulência/genética
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Fungal); 0 (Virulence Factors)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170425
[Lr] Data última revisão:
170425
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170415
[St] Status:MEDLINE
[do] DOI:10.3389/fcimb.2017.00105


  2 / 288 MEDLINE  
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[PMID]:28176377
[Au] Autor:Dukik K; Muñoz JF; Jiang Y; Feng P; Sigler L; Stielow JB; Freeke J; Jamalian A; Gerrits van den Ende B; McEwen JG; Clay OK; Schwartz IS; Govender NP; Maphanga TG; Cuomo CA; Moreno LF; Kenyon C; Borman AM; de Hoog S
[Ad] Endereço:CBS-KNAW Fungal Biodiversity Centre, Utrecht, The Netherlands.
[Ti] Título:Novel taxa of thermally dimorphic systemic pathogens in the Ajellomycetaceae (Onygenales).
[So] Source:Mycoses;60(5):296-309, 2017 May.
[Is] ISSN:1439-0507
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Recent discoveries of novel systemic fungal pathogens with thermally dimorphic yeast-like phases have challenged the current taxonomy of the Ajellomycetaceae, a family currently comprising the genera Blastomyces, Emmonsia, Emmonsiellopsis, Helicocarpus, Histoplasma, Lacazia and Paracoccidioides. Our morphological, phylogenetic and phylogenomic analyses demonstrated species relationships and their specific phenotypes, clarified generic boundaries and provided the first annotated genome assemblies to support the description of two new species. A new genus, Emergomyces, accommodates Emmonsia pasteuriana as type species, and the new species Emergomyces africanus, the aetiological agent of case series of disseminated infections in South Africa. Both species produce small yeast cells that bud at a narrow base at 37°C and lack adiaspores, classically associated with the genus Emmonsia. Another novel dimorphic pathogen, producing broad-based budding cells at 37°C and occurring outside North America, proved to belong to the genus Blastomyces, and is described as Blastomyces percursus.
[Mh] Termos MeSH primário: Micoses/microbiologia
Onygenales/classificação
Onygenales/genética
[Mh] Termos MeSH secundário: Blastomyces/genética
Chrysosporium/genética
Genoma Fúngico
Histoplasma/genética
Seres Humanos
Microscopia
Micélio/ultraestrutura
Micoses/epidemiologia
América do Norte/epidemiologia
Onygenales/patogenicidade
Onygenales/ultraestrutura
Fenótipo
Filogenia
Análise de Sequência de DNA
África do Sul/epidemiologia
Esporos Fúngicos/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170424
[Lr] Data última revisão:
170424
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170209
[St] Status:MEDLINE
[do] DOI:10.1111/myc.12601


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[PMID]:27885567
[Au] Autor:Bohacz J
[Ad] Endereço:Department of Environmental Microbiology, Laboratory of Mycology, Faculty of Agrobioengineering, University of Life Sciences in Lublin, 7 Leszczynskiego Street, 20-069, Lublin, Poland. justyna.bohacz@up.lublin.pl.
[Ti] Título:Biodegradation of feather waste keratin by a keratinolytic soil fungus of the genus Chrysosporium and statistical optimization of feather mass loss.
[So] Source:World J Microbiol Biotechnol;33(1):13, 2017 Jan.
[Is] ISSN:1573-0972
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:This paper assesses the ability of strains of Aphanoascus fulvescens and Chrysosporium articulatum isolated from soil (phaesol) to degrade native feather keratin. Strains were identified based on phenotypic traits and nucleotide sequencing. Response Surface Methodology was used to optimize cultivation conditions exhibiting the highest keratinolytic activity. The experiments were based on Box-Behnken designs for the loss of substrate mass (chicken feathers). While substrate mass loss is an "economic coefficient" that reliably indicates feather keratin degradation, it has not been studied before. Stationary liquid cultures of five selected strains were conducted in laboratory conditions at 28 °C using poultry feathers (1 g) as the sole source of carbon, nitrogen and energy. Enzymatic activities, keratin mineralization products and substrate mass loss were determined periodically. The mineralization of keratin proteins by strains yielded a high number of ammonium ions alkalinizing the medium. Increased ammonium ions inhibited the activity of caseinian protease and keratinase. A decrease in the concentration of these ions induced proteolytic enzymes, chiefly the activity of keratinase, at the end of fungal cultivation. Keratinase activity was related to protein- and peptide release and that of caseinian protease to sulfate ions. The highest loss of substrate mass in comparison to the reference strain CBS104.62 (35.4%) was recorded for Aphanoascus fulvescens B21/4-5 (65.9%). Based on a Box-Behnken design, the maximum loss of substrate mass for the Aphanoascus fulvescens strain (71.08%) can be achieved at pH 7.58 and temperature 28.7 °C.
[Mh] Termos MeSH primário: Chrysosporium/crescimento & desenvolvimento
Chrysosporium/isolamento & purificação
Plumas/metabolismo
Saccharomycetales/crescimento & desenvolvimento
Saccharomycetales/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Biodegradação Ambiental
Carbono/metabolismo
Chrysosporium/genética
Proteínas Fúngicas/metabolismo
Hidrogênio
Resíduos Industriais
Peptídeo Hidrolases/metabolismo
Saccharomycetales/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungal Proteins); 0 (Industrial Waste); 7440-44-0 (Carbon); 7YNJ3PO35Z (Hydrogen); EC 3.4.- (Peptide Hydrolases); EC 3.4.- (keratinase)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170330
[Lr] Data última revisão:
170330
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161126
[St] Status:MEDLINE


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[PMID]:27698170
[Au] Autor:Last LA; Fenton H; Gonyor-McGuire J; Moore M; Yabsley MJ
[Ad] Endereço:Southeastern Cooperative Wildlife Disease Study, College of Veterinary Medicine (Last, Fenton, Yabsley), University of Georgia, Athens, GAWarnell School of Forestry and Natural Resources (Yabsley), University of Georgia, Athens, GAGeorgia Department of Natural Resources, Thomasville, GA (McGuire)The
[Ti] Título:Snake fungal disease caused by Ophidiomyces ophiodiicola in a free-ranging mud snake (Farancia abacura).
[So] Source:J Vet Diagn Invest;28(6):709-713, 2016 Nov.
[Is] ISSN:1943-4936
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Snake fungal disease is an emerging infectious disease caused by the fungus Ophidiomyces ophiodiicola leading to severe dermatitis and facial disfiguration in numerous free-ranging and captive snakes. A free-ranging mud snake (Farancia abacura) from Bulloch County, Georgia, was presented for autopsy because of facial swelling and emaciation. Extensive ulceration of the skin, which was especially severe on the head, and retained shed were noted on external examination. Microscopic examination revealed severe heterophilic dermatitis with intralesional fungal hyphae and arthroconidia consistent with O. ophiodiicola A skin sample incubated on Sabouraud dextrose agar yielded a white-to-tan powdery fungal culture that was confirmed to be O. ophiodiicola by polymerase chain reaction and sequence analysis. Heavy infestation with adult tapeworms (Ophiotaenia faranciae) was present within the intestine. Various bacterial and fungal species, interpreted to either be secondary invaders or postmortem contaminants, were associated with oral lesions. Although the role of these other organisms in the overall health of this individual is not known, factors such as concurrent infections or immunosuppression should be considered in order to better understand the overall manifestation of snake fungal disease, which remains poorly characterized in its host range and geographic distribution.
[Mh] Termos MeSH primário: Chrysosporium/isolamento & purificação
Dermatomicoses/veterinária
Dermatoses Faciais/veterinária
Serpentes
[Mh] Termos MeSH secundário: Animais
Chrysosporium/genética
Dermatomicoses/diagnóstico
Dermatomicoses/microbiologia
Diagnóstico Diferencial
Dermatoses Faciais/diagnóstico
Dermatoses Faciais/microbiologia
Georgia
Reação em Cadeia da Polimerase/veterinária
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161005
[St] Status:MEDLINE


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[PMID]:26951329
[Au] Autor:Schmidt-Ukaj S; Loncaric I; Spergser J; Richter B; Hochleithner M
[Ad] Endereço:Small Animal Internal Medicine, Department for Companion Animals and Horses (Schmidt-Ukaj) of the Department for Pathobiology, University of Veterinary Medicine Vienna, AustriaInstitute of Microbiology (Loncaric, Spergser) of the Department for Pathobiology, University of Veterinary Medicine Vienna,
[Ti] Título:Dermatomycosis in three central bearded dragons (Pogona vitticeps) associated with Nannizziopsis chlamydospora.
[So] Source:J Vet Diagn Invest;28(3):319-22, 2016 May.
[Is] ISSN:1943-4936
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Chronic dermatomycosis was identified in 3 central bearded dragons (Pogona vitticeps), held as companion animals by the same owner. Clinical signs of dermatomycosis included subcutaneous masses as well as crusty, erosive, and ulcerative skin lesions. The facial region was affected in 2 of the 3 cases. Masses were surgically excised, and histology confirmed necrotizing and granulomatous inflammatory processes associated with fungal hyphae. Two of the bearded dragons were euthanized because of their deteriorating condition. In both cases, postmortem histology confirmed systemic fungal infections despite treatment of 1 animal with itraconazole. In the third bearded dragon, therapy with voriconazole at 10 mg/kg was initially effective, but mycotic lesions reappeared 15 months later. Nannizziopsis chlamydospora was identified by PCR and subsequent DNA sequencing in 2 of these cases.
[Mh] Termos MeSH primário: Chrysosporium/isolamento & purificação
Dermatomicoses/veterinária
Lagartos
[Mh] Termos MeSH secundário: Animais
Antifúngicos/uso terapêutico
Dermatomicoses/diagnóstico
Dermatomicoses/tratamento farmacológico
Dermatomicoses/microbiologia
Diagnóstico Diferencial
Eutanásia Animal
Feminino
Itraconazol/uso terapêutico
Masculino
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 304NUG5GF4 (Itraconazole)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160309
[St] Status:MEDLINE
[do] DOI:10.1177/1040638716636422


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[PMID]:26808720
[Au] Autor:Paduch R; Trytek M; Król SK; Kud J; Frant M; Kandefer-Szerszen M; Fiedurek J
[Ad] Endereço:a Department of Virology and Immunology , Institute of Microbiology and Biotechnology, Maria Curie-Sklodowska University , Lublin , Poland ;
[Ti] Título:Biological activity of terpene compounds produced by biotechnological methods.
[So] Source:Pharm Biol;54(6):1096-107, 2016.
[Is] ISSN:1744-5116
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:CONTEXT: Biotransformation systems are profitable tools for structural modification of bioactive natural compounds into valuable biologically active terpenoids. OBJECTIVE: This study determines the biological effect of (R)-(+)-limonene and (-)-α-pinene, and their oxygenated derivatives, (a) perillyl alcohol and (S)-(+)- and (R)-(-)-carvone enantiomers and (b) linalool, trans-verbenol and verbenone, respectively, on human colon tumour cells and normal colonic epithelium. MATERIALS AND METHODS: Biotransformation procedures and in vitro cell culture tests were used in this work. Cells were incubated for 24 h with terpenes at concentrations of 5-500 µg/mL for NR, MTT, DPPH, and NO assays. IL-6 was determined by ELISA with/without 2 h pre-activation with 10 µg/mL LPS. RESULTS: trans-Verbenol and perillyl alcohol, obtained via biotransformation, produced in vitro effect against tumour cells at lower concentrations (IC50 value = 77.8 and 98.8 µg/mL, respectively) than their monoterpene precursors, (R)-(+)-limonene (IC50 value = 171.4 µg/mL) and (-)-α-pinene (IC50 value = 206.3 µg/mL). They also showed lower cytotoxicity against normal cells (IC50 > 500 and > 200 µg/mL, respectively). (S)-(+)-Carvone was 59.4% and 27.1% more toxic to tumour and normal cells, respectively, than the (R)-(-)-enantiomer. (R)-(+)-limonene derivatives decreased IL-6 production from normal cells in media with or without LPS (30.2% and 13.9%, respectively), while (-)-α-pinene derivatives induced IL-6 (verbenone had the strongest effect, 60.2% and 29.1% above control, respectively). None of the terpenes had antioxidative activity below 500 µg/mL. DISCUSSION AND CONCLUSIONS: Bioactivity against tumour cells decreased in the following order: alcohols > ketones > hydrocarbons. (R)-(+)-limonene, (-)-α-pinene, and their derivatives expressed diverse activity towards normal and tumour cells with noticeable enantiomeric differences.
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
Biotecnologia/métodos
Descoberta de Drogas/métodos
Terpenos/farmacologia
[Mh] Termos MeSH secundário: Antineoplásicos/isolamento & purificação
Antineoplásicos/metabolismo
Antineoplásicos/toxicidade
Biotransformação
Compostos de Bifenilo/química
Sobrevivência Celular/efeitos dos fármacos
Chrysosporium/metabolismo
Colo/efeitos dos fármacos
Colo/patologia
Células HT29
Seres Humanos
Mucosa Intestinal/efeitos dos fármacos
Mucosa Intestinal/patologia
Mortierella/metabolismo
Óxido Nítrico/metabolismo
Picratos/química
Terpenos/isolamento & purificação
Terpenos/metabolismo
Terpenos/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Biphenyl Compounds); 0 (Picrates); 0 (Terpenes); 31C4KY9ESH (Nitric Oxide); DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170107
[Lr] Data última revisão:
170107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160126
[St] Status:MEDLINE
[do] DOI:10.3109/13880209.2015.1103753


  7 / 288 MEDLINE  
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[PMID]:26647904
[Au] Autor:Malik R; Capoor MR; Vanidassane I; Gogna A; Singh A; Sen B; Rudramurthy SM; Honnavar P; Gupta S; Chakrabarti A
[Ad] Endereço:Department of Medicine, Vardhman Mahavir Medical College (VMMC) and Safdarjung Hospital, New Delhi, India.
[Ti] Título:Disseminated Emmonsia pasteuriana infection in India: a case report and a review.
[So] Source:Mycoses;59(2):127-32, 2016 Feb.
[Is] ISSN:1439-0507
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:We report here the first case of disseminated Emmonsia pasteuriana infection in a patient with AIDS in India. The patient presented with weight loss, dyspnoea, left-sided chest pain and multiple non-tender skin lesions over face and body for 3 months. Disseminated emmonsiosis was diagnosed on microscopic examination and fungal culture of skin biopsy and needle aspirate of lung consolidation. It was confirmed by sequencing internal transcribed spacer region of rDNA, beta tubulin, actin, and intein PRP8. The patient responded to amphotericin B and itraconazole therapy.
[Mh] Termos MeSH primário: Infecções Oportunistas Relacionadas com a AIDS/diagnóstico
Chrysosporium/isolamento & purificação
Micoses/diagnóstico
[Mh] Termos MeSH secundário: Infecções Oportunistas Relacionadas com a AIDS/tratamento farmacológico
Infecções Oportunistas Relacionadas com a AIDS/microbiologia
Adulto
Anfotericina B/uso terapêutico
Antifúngicos/uso terapêutico
Biópsia por Agulha
Dor no Peito/microbiologia
Chrysosporium/classificação
Chrysosporium/genética
DNA Fúngico/isolamento & purificação
DNA Ribossômico/isolamento & purificação
Erros de Diagnóstico
Dispneia/microbiologia
Feminino
Seres Humanos
Índia/epidemiologia
Itraconazol/uso terapêutico
Micoses/tratamento farmacológico
Micoses/microbiologia
Filogenia
Perda de Peso
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (DNA, Fungal); 0 (DNA, Ribosomal); 304NUG5GF4 (Itraconazole); 7XU7A7DROE (Amphotericin B)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:160113
[Lr] Data última revisão:
160113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151210
[St] Status:MEDLINE
[do] DOI:10.1111/myc.12437


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[PMID]:26346376
[Au] Autor:Chandrakar S; Dias M; Shetty P; Tiewsoh JB; Guarro J
[Ad] Endereço:Department of Microbiology, Father Muller Medical College, Mangalore, 575002, India. sagar88doc@gmail.com.
[Ti] Título:Isolation and Characterization of an Unknown Chrysosporium sp. Producing Subcutaneous Mycosis in an Immunocompromised Patient.
[So] Source:Mycopathologia;181(1-2):115-8, 2016 Feb.
[Is] ISSN:1573-0832
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:We report a case of subcutaneous fungal abscess over the great toe caused by a keratinophilic fungus, an unknown Chrysosporium sp., in a 60-year-old diabetic female who was treated successfully with oral fluconazole. The fungus was isolated from aspirated pus, and septate hyphae were seen in fine needle aspiration cytology. Ovoid- to club-shaped hyaline one-celled conidia (aleuriconidia) with broad truncated bases were seen, and sequencing of ITS1-5.8S-ITS2 rDNA revealed belonging to the order Onygenales and most closely related to Chrysosporium spp. isolated from a fowl. Of the 65 species within the genus Chrysosporium, very few have been reported as pathogenic.
[Mh] Termos MeSH primário: Abscesso/etiologia
Abscesso/patologia
Chrysosporium/isolamento & purificação
Dermatomicoses/diagnóstico
Dermatomicoses/patologia
Hospedeiro Imunocomprometido
[Mh] Termos MeSH secundário: Abscesso/microbiologia
Antifúngicos/administração & dosagem
Biópsia por Agulha Fina
Chrysosporium/classificação
Chrysosporium/genética
DNA Fúngico/química
DNA Fúngico/genética
DNA Espaçador Ribossômico/química
DNA Espaçador Ribossômico/genética
Dermatomicoses/microbiologia
Complicações do Diabetes
Feminino
Fluconazol/administração & dosagem
Seres Humanos
Técnicas Microbiológicas
Meia-Idade
Dados de Sequência Molecular
Análise de Sequência de DNA
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (DNA, Fungal); 0 (DNA, Ribosomal Spacer); 8VZV102JFY (Fluconazole)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150909
[St] Status:MEDLINE
[do] DOI:10.1007/s11046-015-9883-4


  9 / 288 MEDLINE  
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[PMID]:26584311
[Au] Autor:Schwartz IS; Kenyon C; Feng P; Govender NP; Dukik K; Sigler L; Jiang Y; Stielow JB; Muñoz JF; Cuomo CA; Botha A; Stchigel AM; de Hoog GS
[Ad] Endereço:International Health Unit, Department of Epidemiology and Social Medicine, University of Antwerp, Antwerp, Belgium.
[Ti] Título:50 Years of Emmonsia Disease in Humans: The Dramatic Emergence of a Cluster of Novel Fungal Pathogens.
[So] Source:PLoS Pathog;11(11):e1005198, 2015.
[Is] ISSN:1553-7374
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Chrysosporium/patogenicidade
Fungos/patogenicidade
Micoses/microbiologia
Esporos Fúngicos/genética
Esporos Fúngicos/patogenicidade
[Mh] Termos MeSH secundário: Animais
Chrysosporium/genética
Fungos/genética
Variação Genética/genética
Seres Humanos
Micoses/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Em] Mês de entrada:1604
[Cu] Atualização por classe:170910
[Lr] Data última revisão:
170910
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151120
[St] Status:MEDLINE
[do] DOI:10.1371/journal.ppat.1005198


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[PMID]:26439490
[Au] Autor:Muñoz JF; Gauthier GM; Desjardins CA; Gallo JE; Holder J; Sullivan TD; Marty AJ; Carmen JC; Chen Z; Ding L; Gujja S; Magrini V; Misas E; Mitreva M; Priest M; Saif S; Whiston EA; Young S; Zeng Q; Goldman WE; Mardis ER; Taylor JW; McEwen JG; Clay OK; Klein BS; Cuomo CA
[Ad] Endereço:Cellular and Molecular Biology Unit, Corporación para Investigaciones Biológicas, Medellín, Colombia; Institute of Biology, Universidad de Antioquia, Medellín, Colombia.
[Ti] Título:The Dynamic Genome and Transcriptome of the Human Fungal Pathogen Blastomyces and Close Relative Emmonsia.
[So] Source:PLoS Genet;11(10):e1005493, 2015 Oct.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Three closely related thermally dimorphic pathogens are causal agents of major fungal diseases affecting humans in the Americas: blastomycosis, histoplasmosis and paracoccidioidomycosis. Here we report the genome sequence and analysis of four strains of the etiological agent of blastomycosis, Blastomyces, and two species of the related genus Emmonsia, typically pathogens of small mammals. Compared to related species, Blastomyces genomes are highly expanded, with long, often sharply demarcated tracts of low GC-content sequence. These GC-poor isochore-like regions are enriched for gypsy elements, are variable in total size between isolates, and are least expanded in the avirulent B. dermatitidis strain ER-3 as compared with the virulent B. gilchristii strain SLH14081. The lack of similar regions in related species suggests these isochore-like regions originated recently in the ancestor of the Blastomyces lineage. While gene content is highly conserved between Blastomyces and related fungi, we identified changes in copy number of genes potentially involved in host interaction, including proteases and characterized antigens. In addition, we studied gene expression changes of B. dermatitidis during the interaction of the infectious yeast form with macrophages and in a mouse model. Both experiments highlight a strong antioxidant defense response in Blastomyces, and upregulation of dioxygenases in vivo suggests that dioxide produced by antioxidants may be further utilized for amino acid metabolism. We identify a number of functional categories upregulated exclusively in vivo, such as secreted proteins, zinc acquisition proteins, and cysteine and tryptophan metabolism, which may include critical virulence factors missed before in in vitro studies. Across the dimorphic fungi, loss of certain zinc acquisition genes and differences in amino acid metabolism suggest unique adaptations of Blastomyces to its host environment. These results reveal the dynamics of genome evolution and of factors contributing to virulence in Blastomyces.
[Mh] Termos MeSH primário: Blastomyces/genética
Chrysosporium/genética
Genoma Fúngico
Transcriptoma/genética
[Mh] Termos MeSH secundário: Animais
Blastomyces/patogenicidade
Blastomicose/genética
Blastomicose/microbiologia
Chrysosporium/patogenicidade
Histoplasmose/genética
Histoplasmose/microbiologia
Seres Humanos
Macrófagos/microbiologia
Camundongos
Paracoccidioidomicose/genética
Paracoccidioidomicose/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1605
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151007
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1005493



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