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[PMID]:28566083
[Au] Autor:Basaran S; Karabiçak N; Simsek Yavuz S; Wiederhold NP; Safak S; Saribuga A; Sutton DA; Bingöl Z; Çagatay A; Özsüt H; Kiliçaslan Z; Eraksoy H
[Ad] Endereço:Istanbul University Istanbul Faculty of Medicine, Department of Infectious Diseases and Clinical Microbiology, Istanbul, Turkey. senihabasaran@yahoo.com.
[Ti] Título:[A case of coccidioidomycosis in Turkey imported from the United States of America].
[Ti] Título:Türkiye'de Amerika Birlesik Devletleri kaynakli bir koksidiyoidomikoz olgusu..
[So] Source:Mikrobiyol Bul;51(2):183-190, 2017 Apr.
[Is] ISSN:0374-9096
[Cp] País de publicação:Turkey
[La] Idioma:tur
[Ab] Resumo:Coccidioidomycosis caused by Coccidioides immitis or Coccidioides posadasii is a rare infectious disease except in endemic regions. In this report the third documented imported case of coccidioidomycosis in Turkey was presented. A thirty-year-old male patient was admitted to our hospital with fever and purulent drainage from his chest tube. He had worked in Arizona, USA, until 4 months before this presentation. While in Arizona, he experienced cough and hemoptysis and was diagnosed as pulmonary coccidioidomycosis. He was treated with itraconazole for two months and he had no symptoms for 3 years. He then returned to Turkey and 2 months after his return to Turkey, he was admitted to another hospital in Istanbul with dyspnea and diagnosed as hydro-pneumothorax, and pleural fluid obtained from the inserted chest tube was found to be purulent. One gram of BID amoxicillin-clavulanate was given. Physical examination on admission revealed a purulent drainage on the right side chest tube, a temperature of 38.5°C and decreased breath sounds on the right lung. Piperacillin-tazobactam 3 x 4.5 g intravenous and fluconazole 400 mg intravenous once daily were started. Human immunodeficiency virus test was negative. Gram-negative diplococci and rods, gram-positive cocci and septate hyphae were seen in the Gram stain of his pleural fluid. Pleural fluid culture revealed Moraxella catarrhalis after 24 hours incubation and a mold after 72 hours of incubation. Anti-coccidioidal antibodies were found positive in a titer of 1/2. Hydro-pneumothorax, atelectasis and a 3 mm nodules in the right lung were seen in his thorax CT. The patient's pleural fluid and the culture plates were sent to the Public Health Institute of Turkey, Mycology Reference Laboratory (PHIT-MRL), with a clinical suspicion of coccidioidomycosis. The specimen and plates were submitted to the PHIT-MRL Bio Safety Level-3 laboratory for mycological evaluation. The microscopic examination of 15% KOH preparations of pleural fluid specimens revealed septate hyphae which appear to be in the early stages of forming arthroconidia. The pleural fluid culture grew buff-white coloured colonies with aerial hyphae, which were suspected of being a Coccidioides spp. The strain was identified as C.immitis/posadasii by direct microscopy and culture, and subsequently confirmed by the FDA-approved DNA probe. DNA sequence analysis of the ITS and D1/D2 rDNA regions confirmed the isolate to be C.posadasii species [ITS 100% match to GenBank Accession No. AB232901 (630/630 base pair match), and D1/D2 100% match to GenBank Accession No. AB232884 (617/617 base pair match)]. ITS1 and ITS2 barcode analysis also confirmed the species to be C.posadasii, which is the species endemic in Arizona. Susceptibility testing was performed according to Clinical and Laboratory Standards Institute M38-A2 guidelines in the Fungus Testing Laboratory of the University of Texas Health Science Center at San Antonio and minimal inhibitory concentration values were; 0.125 µg/ml for amphotericin B, posaconazole and voriconazole, 0.5 µg/ml for itraconazole and 8 µg/ml for fluconazole. He had decortication of the pleura and was discharged from hospital after six weeks treatment with intravenous fluconazole which was continued orally for one year. Anti-coccidioidal antibodies were negative after two months of treatment. The patient is currently asymptomatic. The presented case is the third case reported from Turkey and provides additional contribution to the existing literature with regard to the appearance of arthroconidium, which is the unusual hyphal form, instead of the expected spherules in the infected tissue.
[Mh] Termos MeSH primário: Antifúngicos/uso terapêutico
Coccidioides/isolamento & purificação
Coccidioidomicose/microbiologia
[Mh] Termos MeSH secundário: Adulto
Combinação Amoxicilina e Clavulanato de Potássio/farmacologia
Combinação Amoxicilina e Clavulanato de Potássio/uso terapêutico
Antifúngicos/farmacologia
Arizona
Coccidioides/efeitos dos fármacos
Coccidioides/crescimento & desenvolvimento
Coccidioidomicose/tratamento farmacológico
Fluconazol/farmacologia
Fluconazol/uso terapêutico
Seres Humanos
Itraconazol/farmacologia
Itraconazol/uso terapêutico
Masculino
Ácido Penicilânico/análogos & derivados
Ácido Penicilânico/farmacologia
Ácido Penicilânico/uso terapêutico
Piperacilina/farmacologia
Piperacilina/uso terapêutico
Pleura/microbiologia
Recidiva
Esporos Fúngicos/efeitos dos fármacos
Esporos Fúngicos/crescimento & desenvolvimento
Esporos Fúngicos/isolamento & purificação
Viagem
Turquia
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 157044-21-8 (piperacillin, tazobactam drug combination); 304NUG5GF4 (Itraconazole); 74469-00-4 (Amoxicillin-Potassium Clavulanate Combination); 87-53-6 (Penicillanic Acid); 8VZV102JFY (Fluconazole); X00B0D5O0E (Piperacillin)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170602
[St] Status:MEDLINE


  2 / 1126 MEDLINE  
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[PMID]:28380017
[Au] Autor:Chow NA; Lindsley MD; McCotter OZ; Kangiser D; Wohrle RD; Clifford WR; Yaglom HD; Adams LE; Komatsu K; Durkin MM; Baker RJ; Shubitz LF; Derado G; Chiller TM; Litvintseva AP
[Ad] Endereço:Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America.
[Ti] Título:Development of an enzyme immunoassay for detection of antibodies against Coccidioides in dogs and other mammalian species.
[So] Source:PLoS One;12(4):e0175081, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Coccidioides is a soil-dwelling fungus that causes coccidioidomycosis, a disease also known as Valley fever, which affects humans and a variety of animal species. Recent findings of Coccidioides in new, unexpected areas of the United States have demonstrated the need for a better understanding of its geographic distribution. Large serological studies on animals could provide important information on the geographic distribution of this pathogen. To facilitate such studies, we used protein A/G, a recombinant protein that binds IgG antibodies from a variety of mammalian species, to develop an enzyme immunoassay (EIA) that detects IgG antibodies against Coccidioides in a highly sensitive and high-throughput manner. We showed the potential of this assay to be adapted to multiple animal species by testing a collection of serum and/or plasma samples from dogs, mice, and humans with or without confirmed coccidioidomycosis. We then evaluated the performance of the assay in dogs, using sera from dogs residing in a highly endemic area, and found seropositivity rates significantly higher than those in dogs of non-endemic areas. We further evaluated the specificity of the assay in dogs infected with other fungal pathogens known to cross-react with Coccidioides. Finally, we used the assay to perform a cross-sectional serosurvey investigating dogs from Washington, a state in which infection with Coccidioides has recently been documented. In summary, we have developed a Coccidioides EIA for the detection of antibodies in canines that is more sensitive and has higher throughput than currently available methods, and by testing this assay in mice and humans, we have shown a proof of principle of its adaptability for other animal species.
[Mh] Termos MeSH primário: Anticorpos Antifúngicos/imunologia
Coccidioides/imunologia
Coccidioidomicose/veterinária
Doenças do Cão/diagnóstico
Técnicas Imunoenzimáticas/veterinária
[Mh] Termos MeSH secundário: Animais
Coccidioidomicose/diagnóstico
Coccidioidomicose/epidemiologia
Coccidioidomicose/imunologia
Estudos Transversais
Doenças do Cão/epidemiologia
Doenças do Cão/imunologia
Doenças do Cão/microbiologia
Cães
Imunodifusão/veterinária
Técnicas Imunoenzimáticas/métodos
Washington/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Fungal)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170406
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0175081


  3 / 1126 MEDLINE  
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[PMID]:28096163
[Au] Autor:Thompson GR; Barker BM; Wiederhold NP
[Ad] Endereço:Department of Medicine, Division of Infectious Diseases, University of California-Davis Medical Center, Sacramento, California, USA grthompson@ucdavis.edu.
[Ti] Título:Large-Scale Evaluation of Amphotericin B, Triazole, and Echinocandin Activity against Coccidioides Species from U.S. Institutions.
[So] Source:Antimicrob Agents Chemother;61(4), 2017 Apr.
[Is] ISSN:1098-6596
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Large-scale testing of isolates has not been performed, and the frequency of clinical isolates with elevated amphotericin B or triazole MICs has not been evaluated. isolates ( = 581) underwent antifungal susceptibility testing. Elevated MIC values were observed for fluconazole (≥16 µg/ml, 37.3% of isolates; ≥32 µg/ml, 7.9% of isolates), itraconazole (≥2 µg/ml, 1.0% of isolates), posaconazole (≥1 µg/ml, 1.0% of isolates), and voriconazole (≥2 µg/ml, 1.2% of isolates). However, mold-active triazoles exhibited low MICs for the majority of isolates tested. Additional correlation with patient outcomes to determine the relevance of elevated MICs in isolates is needed.
[Mh] Termos MeSH primário: Anfotericina B/farmacologia
Antifúngicos/farmacologia
Coccidioides/efeitos dos fármacos
Equinocandinas/farmacologia
Triazóis/farmacologia
[Mh] Termos MeSH secundário: Coccidioidomicose/microbiologia
Flucitosina/farmacologia
Itraconazol/farmacologia
Lipopeptídeos/farmacologia
Testes de Sensibilidade Microbiana
Estados Unidos
Voriconazol/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Echinocandins); 0 (Lipopeptides); 0 (Triazoles); 304NUG5GF4 (Itraconazole); 6TK1G07BHZ (posaconazole); 7XU7A7DROE (Amphotericin B); D83282DT06 (Flucytosine); F0XDI6ZL63 (caspofungin); JFU09I87TR (Voriconazole)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170119
[St] Status:MEDLINE


  4 / 1126 MEDLINE  
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[PMID]:28053216
[Au] Autor:Malo J; Holbrook E; Zangeneh T; Strawter C; Oren E; Robey I; Erickson H; Chahal R; Durkin M; Thompson C; Hoover SE; Ampel NM; Wheat LJ; Knox KS
[Ad] Endereço:Department of Medicine, University of Arizona College of Medicine, Tucson, Arizona, USA jmalo@deptofmed.arizona.edu.
[Ti] Título:Enhanced Antibody Detection and Diagnosis of Coccidioidomycosis with the MiraVista IgG and IgM Detection Enzyme Immunoassay.
[So] Source:J Clin Microbiol;55(3):893-901, 2017 Mar.
[Is] ISSN:1098-660X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Coccidioidomycosis is a common cause of community-acquired pneumonia in areas of the southwestern United States in which the disease is endemic. Clinical presentations range from self-limited disease to severe disseminated disease. Therefore, early and accurate diagnosis is essential to ensure appropriate treatment and monitoring. Currently available diagnostic tests have variable accuracy, particularly in certain patient populations, and new tests may offer improved accuracy for the diagnosis of coccidioidomycosis. Serum samples from 103 cases of coccidioidomycosis and 373 controls were tested for IgG and IgM antibodies using the MVista anti- antibody enzyme immunoassay. Serum specimens from 170 controls from areas in which the disease is endemic and 44 cases were tested by immunodiffusion at MiraVista Diagnostics. The sensitivity of the MVista antibody assay was 88.3%, and the specificity was 90%. The sensitivity was maintained in the presence of immunocompromising conditions or immunosuppressive therapies. The sensitivity of immunodiffusion was 60.2%, and the specificity was 98.8%. The sensitivity of complement fixation (62 cases) was 66.1%, but the specificity could not be determined. The MVista anti- antibody enzyme immunoassay offers improved sensitivity, compared with immunodiffusion and complement fixation, is not impaired in immunocompromised patients, and permits highly reproducible semiquantification.
[Mh] Termos MeSH primário: Anticorpos Antifúngicos/sangue
Coccidioides/imunologia
Coccidioidomicose/diagnóstico
Técnicas Imunoenzimáticas/métodos
Imunoglobulina G/sangue
Imunoglobulina M/sangue
[Mh] Termos MeSH secundário: Infecções Comunitárias Adquiridas/diagnóstico
Seres Humanos
Pneumonia/diagnóstico
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
Estados Unidos
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Fungal); 0 (Immunoglobulin G); 0 (Immunoglobulin M)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170902
[Lr] Data última revisão:
170902
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170106
[St] Status:MEDLINE
[do] DOI:10.1128/JCM.01880-16


  5 / 1126 MEDLINE  
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[PMID]:27646561
[Au] Autor:Goughenour KD; Rappleye CA
[Ad] Endereço:a Department of Microbiology , Ohio State University , Columbus , OH , USA.
[Ti] Título:Antifungal therapeutics for dimorphic fungal pathogens.
[So] Source:Virulence;8(2):211-221, 2017 Feb 17.
[Is] ISSN:2150-5608
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Dimorphic fungi cause several endemic mycoses which range from subclinical respiratory infections to life-threatening systemic disease. Pathogenic-phase cells of Histoplasma, Blastomyces, Paracoccidioides and Coccidioides escape elimination by the innate immune response with control ultimately requiring activation of cell-mediated immunity. Clinical management of disease relies primarily on antifungal compounds; however, dimorphic fungal pathogens create a number of challenges for antifungal drug therapy. In addition to the drug toxicity issues known for current antifungals, barriers to efficient drug treatment of dimorphic fungal infections include natural resistance to the echinocandins, residence of fungal cells within immune cells, the requirement for systemic delivery of drugs, prolonged treatment times, potential for latent infections, and lack of optimized standardized methodology for in vitro testing of drug susceptibilities. This review will highlight recent advances, current therapeutic options, and new compounds on the horizon for treating infections by dimorphic fungal pathogens.
[Mh] Termos MeSH primário: Antifúngicos/uso terapêutico
Blastomicose/tratamento farmacológico
Coccidioidomicose/tratamento farmacológico
Descoberta de Drogas
Histoplasmose/tratamento farmacológico
Paracoccidioidomicose/tratamento farmacológico
[Mh] Termos MeSH secundário: Aminoglicosídeos/uso terapêutico
Antifúngicos/efeitos adversos
Azóis/uso terapêutico
Blastomyces/efeitos dos fármacos
Blastomyces/imunologia
Blastomicose/microbiologia
Coccidioides/efeitos dos fármacos
Coccidioides/imunologia
Coccidioidomicose/microbiologia
Farmacorresistência Fúngica
Equinocandinas/uso terapêutico
Histoplasma/efeitos dos fármacos
Histoplasma/imunologia
Histoplasmose/microbiologia
Seres Humanos
Paracoccidioides/efeitos dos fármacos
Paracoccidioides/imunologia
Paracoccidioidomicose/microbiologia
Polienos/uso terapêutico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aminoglycosides); 0 (Antifungal Agents); 0 (Azoles); 0 (Echinocandins); 0 (Polyenes); 9Z22C3QQCJ (nikkomycin)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160921
[St] Status:MEDLINE
[do] DOI:10.1080/21505594.2016.1235653


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[PMID]:27903576
[Au] Autor:Majeed A; Ullah W; Hamadani AA; Georgescu A
[Ad] Endereço:Infectious Disease Division, Department of Medicine, University of Arizona, Tucson, Arizona, USA.
[Ti] Título:First reported case of peroneal tenosynovitis caused by Coccidioides immitis successfully treated with fluconazole.
[So] Source:BMJ Case Rep;2016, 2016 Nov 30.
[Is] ISSN:1757-790X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Coccidioidomycosis is an insidious infection caused by Coccidioides spp (C. immitis and C. posadasii). Disseminated disease occasionally involves tendon sheaths and synovium of the joints leading to tenosynovitis. Here, we describe the case of a 72-year-old woman from southern Arizona, who presented with right ankle pain, redness and swelling for 2 months. Her serum IgG was positive for C. immitis on complement fixation, and her MRI of the right ankle joint showed extensive tenosynovitis of the right peroneal tendons, and subtalar joint effusions with associated synovitis. The purpose of this case is to report an extremely rare manifestation of disseminated C. immitis, that is, peroneal tenosynovitis and challenges involved with diagnosis and treatment. We also document that azole therapy is an effective treatment option for peroneal tenosynovitis caused by C. immitis, and we had to double the dose for slow symptom resolution with 4-week trial with usual 400 mg oral dose of fluconazole.
[Mh] Termos MeSH primário: Antifúngicos/uso terapêutico
Coccidioides
Coccidioidomicose/tratamento farmacológico
Fluconazol/uso terapêutico
Neuropatias Fibulares/tratamento farmacológico
Tenossinovite/tratamento farmacológico
[Mh] Termos MeSH secundário: Idoso
Articulação do Tornozelo/microbiologia
Coccidioidomicose/microbiologia
Feminino
Seres Humanos
Neuropatias Fibulares/microbiologia
Tenossinovite/microbiologia
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 8VZV102JFY (Fluconazole)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170308
[Lr] Data última revisão:
170308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161202
[St] Status:MEDLINE


  7 / 1126 MEDLINE  
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[PMID]:27724885
[Au] Autor:Del Rocío Reyes-Montes M; Pérez-Huitrón MA; Ocaña-Monroy JL; Frías-De-León MG; Martínez-Herrera E; Arenas R; Duarte-Escalante E
[Ad] Endereço:Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), Ciudad Universitaria No. 3000, Coyoacán, 04510, México Cd.Mx., Mexico.
[Ti] Título:The habitat of Coccidioides spp. and the role of animals as reservoirs and disseminators in nature.
[So] Source:BMC Infect Dis;16(1):550, 2016 Oct 10.
[Is] ISSN:1471-2334
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Coccidioidomycosis, a potentially fatal fungal infection, is considered an emergent mycotic disease because of the increased incidence of fungal infections registered over recent years. Infection occurs through the inhalation of arthroconidia from two main species of Coccidioides: Coccidioides immitis and C. posadasii, which are both endemic to arid and semi-arid regions of North America. Coccidioides species not only infect humans but can also infect other mammals (land, aquatic, wild or domestic), reptiles and birds. OBJECTIVE: To obtain information regarding the habitat of Coccidioides spp. and the animals infected by this fungus and to identify the role that infected animals play as reservoirs and disseminators of this fungus in nature. MATERIALS: A literature review was conducted to identify the habitat of Coccidioides spp. and the infected non-human animal species targeted by this fungus. RESULTS AND CONCLUSIONS: This review allows us to suggest that Coccidioides spp. may be classified as halotolerant organisms; nevertheless, to perpetuate their life cycle, these organisms depend on different animal species (reservoirs) that serve as a link with the environment, by acting as disseminators of the fungi in nature.
[Mh] Termos MeSH primário: Coccidioides/fisiologia
Coccidioidomicose/transmissão
Reservatórios de Doenças
Vetores de Doenças
Ecossistema
[Mh] Termos MeSH secundário: Animais
Coccidioidomicose/microbiologia
Seres Humanos
América do Norte
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170531
[Lr] Data última revisão:
170531
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161012
[St] Status:MEDLINE


  8 / 1126 MEDLINE  
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[PMID]:27546806
[Au] Autor:Grys TE; Kaushal S; Chowdhury Y; Dasari S; Mitchell NM; Magee DM; Blair JE; Colby TV; Lake DF
[Ad] Endereço:Department of Laboratory Medicine and Pathology, Mayo Clinic , Phoenix, Arizona 85054, United States.
[Ti] Título:Total and Lectin-Binding Proteome of Spherulin from Coccidioides posadasii.
[So] Source:J Proteome Res;15(10):3463-3472, 2016 Oct 07.
[Is] ISSN:1535-3907
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Coccidioides is a virulent dimorphic fungus that causes coccidioidomycosis (valley fever) in mammals, including humans. Although the genome has been sequenced, a proteomic analysis does not exist. To address this gap in proteomic knowledge, we generated the proteome of spherulin (a well-studied lysate of fungal spherules) and identified 1390 proteins. Some of the proteins included glycosylation enzymes, which led us to hypothesize that fungal glycosylation patterns may be different from those of mammals and could be exploited to detect Coccidioides in tissues. We performed lectin-based immunohistochemistry on formalin-fixed paraffin-embedded human patients' lung tissues. GSL-II (Griffonia simplificonia lectin II) and sWGA (succinylated wheat germ agglutinin) lectins bound specifically to endospores and spherules in infected lungs. To identify lectin-binding glycoproteins in spherulin, we performed lectin-affinity chromatography, followed by LC-MS/MS. A total of 195 glycoproteins from spherulin bound to GSL-II, 224 glycoproteins bound to sWGA, and 145 glycoproteins bound to both lectins. This is the first report of the specific reactivity of GSL-II and sWGA lectins to Coccidioides endospores and spherules in infected human tissues and the first listing of the Coccidioidal proteome from spherulin using sequences present in three Coccidioides databases: RefSeq, SwissProt, and The Broad Institute's Coccidioides Genome project.
[Mh] Termos MeSH primário: Coccidioides/química
Coccidioidina/química
Proteínas Fúngicas/análise
Lectinas/metabolismo
Proteoma/metabolismo
[Mh] Termos MeSH secundário: Cromatografia de Afinidade
Coccidioidomicose/diagnóstico
Coccidioidomicose/patologia
Glicoproteínas/análise
Glicoproteínas/metabolismo
Glicosilação
Seres Humanos
Imuno-Histoquímica
Pulmão/patologia
Ligação Proteica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungal Proteins); 0 (Glycoproteins); 0 (Lectins); 0 (Proteome); 0 (spherulin); 12622-73-0 (Coccidioidin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160823
[St] Status:MEDLINE


  9 / 1126 MEDLINE  
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[PMID]:27492257
[Au] Autor:Remus BS; Schwer B; Shuman S
[Ad] Endereço:Molecular Biology Program, Sloan-Kettering Institute, New York, New York 10065, USA.
[Ti] Título:Characterization of the tRNA ligases of pathogenic fungi Aspergillus fumigatus and Coccidioides immitis.
[So] Source:RNA;22(10):1500-9, 2016 Oct.
[Is] ISSN:1469-9001
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Yeast tRNA ligase (Trl1) is an essential trifunctional enzyme that repairs RNA breaks with 2',3'-cyclic-PO4 and 5'-OH ends. Trl1 is composed of C-terminal cyclic phosphodiesterase and central polynucleotide kinase domains that heal the broken ends to generate the 3'-OH, 2'-PO4, and 5'-PO4 termini required for sealing by an N-terminal ligase domain. Trl1 enzymes are found in all human fungal pathogens and they are promising targets for antifungal drug discovery because: (i) their domain structures and biochemical mechanisms are unique compared to the mammalian RtcB-type tRNA splicing enzyme; and (ii) there are no obvious homologs of the Trl1 ligase domain in mammalian proteomes. Here we characterize the tRNA ligases of two human fungal pathogens: Coccidioides immitis and Aspergillus fumigatus The biological activity of CimTrl1 and AfuTrl1 was verified by showing that their expression complements a Saccharomyces cerevisiae trl1Δ mutant. Purified recombinant AfuTrl1 and CimTrl1 proteins were catalytically active in joining 2',3'-cyclic-PO4 and 5'-OH ends in vitro, either as full-length proteins or as a mixture of separately produced healing and sealing domains. The biochemical properties of CimTrl1 and AfuTrl1 are similar to those of budding yeast Trl1, particularly with respect to their preferential use of GTP as the phosphate donor for the polynucleotide kinase reaction. Our findings provide genetic and biochemical tools to screen for inhibitors of tRNA ligases from pathogenic fungi.
[Mh] Termos MeSH primário: Aspergillus fumigatus/enzimologia
Coccidioides/enzimologia
Proteínas Fúngicas/metabolismo
RNA Ligase (ATP)/metabolismo
[Mh] Termos MeSH secundário: Aspergillus fumigatus/genética
Coccidioides/genética
Proteínas Fúngicas/genética
Guanosina Trifosfato/metabolismo
RNA Ligase (ATP)/genética
Processamento de RNA
RNA de Transferência/genética
RNA de Transferência/metabolismo
Saccharomyces cerevisiae/genética
Saccharomyces cerevisiae/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungal Proteins); 86-01-1 (Guanosine Triphosphate); 9014-25-9 (RNA, Transfer); EC 6.5.1.3 (RNA Ligase (ATP))
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171001
[Lr] Data última revisão:
171001
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160806
[St] Status:MEDLINE
[do] DOI:10.1261/rna.057455.116


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[PMID]:27481239
[Au] Autor:Narra HP; Shubitz LF; Mandel MA; Trinh HT; Griffin K; Buntzman AS; Frelinger JA; Galgiani JN; Orbach MJ
[Ad] Endereço:School of Plant Sciences, The University of Arizona, Tucson, Arizona, USA.
[Ti] Título:A Coccidioides posadasii CPS1 Deletion Mutant Is Avirulent and Protects Mice from Lethal Infection.
[So] Source:Infect Immun;84(10):3007-16, 2016 Oct.
[Is] ISSN:1098-5522
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The CPS1 gene was identified as a virulence factor in the maize pathogen Cochliobolus heterostrophus Hypothesizing that the homologous gene in Coccidioides posadasii could be important for virulence, we created a Δcps1 deletion mutant which was unable to cause disease in three strains of mice (C57BL/6, BALB/c, or the severely immunodeficient NOD-scid,γc(null) [NSG]). Only a single colony was recovered from 1 of 60 C57BL/6 mice following intranasal infections of up to 4,400 spores. Following administration of very high doses (10,000 to 2.5 × 10(7) spores) to NSG and BALB/c mice, spherules were observed in lung sections at time points from day 3 to day 10 postinfection, but nearly all appeared degraded with infrequent endosporulation. Although the role of CPS1 in virulence is not understood, phenotypic alterations and transcription differences of at least 33 genes in the Δcps1 strain versus C. posadasii is consistent with both metabolic and regulatory functions for the gene. The in vitro phenotype of the Δcps1 strain showed slower growth of mycelia with delayed and lower spore production than C. posadasii, and in vitro spherules were smaller. Vaccination of C57BL/6 or BALB/c mice with live Δcps1 spores either intranasally, intraperitoneally, or subcutaneously resulted in over 95% survival with mean residual lung fungal burdens of <1,000 CFU from an otherwise lethal C. posadasii intranasal infection. Considering its apparently complete attenuation of virulence and the high degree of resistance to C. posadasii infection when used as a vaccine, the Δcps1 strain is a promising vaccine candidate for preventing coccidioidomycosis in humans or other animals.
[Mh] Termos MeSH primário: Coccidioides/fisiologia
Coccidioidomicose/genética
Deleção de Sequência
Fatores de Virulência/genética
Virulência/fisiologia
[Mh] Termos MeSH secundário: Animais
Coccidioides/genética
Coccidioidomicose/prevenção & controle
Modelos Animais de Doenças
Feminino
Proteínas Fúngicas/genética
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Endogâmicos C57BL
Camundongos Endogâmicos NOD
Vacinação/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungal Proteins); 0 (Virulence Factors)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160803
[St] Status:MEDLINE
[do] DOI:10.1128/IAI.00633-16



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