Base de dados : MEDLINE
Pesquisa : B01.300.381.258 [Categoria DeCS]
Referências encontradas : 2354 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 236 ir para página                         

  1 / 2354 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29304090
[Au] Autor:Mpoza E; Mukaremera L; Kundura DA; Akampurira A; Luggya T; Tadeo KK; Pastick KA; Bridge SC; Tugume L; Kiggundu R; Musubire AK; Williams DA; Muzoora C; Nalintya E; Rajasingham R; Rhein J; Boulware DR; Meya DB; Abassi M
[Ad] Endereço:Infectious Diseases Institute, Kampala, Uganda.
[Ti] Título:Evaluation of a point-of-care immunoassay test kit 'StrongStep' for cryptococcal antigen detection.
[So] Source:PLoS One;13(1):e0190652, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: HIV-associated cryptococcal meningitis is the leading cause of adult meningitis in Sub-Saharan Africa, accounting for 15%-20% of AIDS-attributable mortality. The development of point-of-care assays has greatly improved the screening and diagnosis of cryptococcal disease. We evaluated a point-of-care immunoassay, StrongStep (Liming Bio, Nanjing, Jiangsu, China) lateral flow assay (LFA), for cryptococcal antigen (CrAg) detection in cerebrospinal fluid (CSF) and plasma. METHODS: We retrospectively tested 143 CSF and 77 plasma samples collected from HIV-seropositive individuals with suspected meningitis from 2012-2016 in Uganda. We prospectively tested 90 plasma samples collected from HIV-seropositive individuals with CD4 cell count <100 cells/µL from 2016-2017 as part of a cryptococcal antigenemia screening program. The StrongStep CrAg was tested against a composite reference standard of positive Immy CrAg LFA (Immy, Norman, OK, USA) or CSF culture with statistical comparison by McNemar's test. RESULTS: StrongStep CrAg had a 98% (54/55) sensitivity and 90% (101/112) specificity in plasma (P = 0.009, versus reference standard). In CSF, the StrongStep CrAg had 100% (101/101) sensitivity and 98% (41/42) specificity (P = 0.99). Adjusting for the cryptococcal antigenemia prevalence of 9% in Uganda and average cryptococcal meningitis prevalence of 37% in Sub-Saharan Africa, the positive predictive value of the StrongStep CrAg was 50% in plasma and 96% in CSF. CONCLUSIONS: We found the StrongStep CrAg LFA to be a sensitive assay, which unfortunately lacked specificity in plasma. In lower prevalence settings, a majority of positive results from blood would be expected to be false positives.
[Mh] Termos MeSH primário: Antígenos de Fungos/sangue
Antígenos de Fungos/líquido cefalorraquidiano
Cryptococcus/imunologia
Imunoensaio/métodos
Testes Imediatos
[Mh] Termos MeSH secundário: Adulto
Idoso
Contagem de Linfócito CD4
Feminino
Seres Humanos
Imunoensaio/normas
Masculino
Meia-Idade
Estudos Retrospectivos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Antigens, Fungal)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180106
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190652


  2 / 2354 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28880897
[Au] Autor:Tian H; Wang H; Hui X; Wang Z; Drijber RA; Liu J
[Ad] Endereço:Key Laboratory of Plant Nutrition and Agro-environment in Northwest China, Ministry of Agriculture/College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi, China.
[Ti] Título:Changes in soil microbial communities after 10 years of winter wheat cultivation versus fallow in an organic-poor soil in the Loess Plateau of China.
[So] Source:PLoS One;12(9):e0184223, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Agricultural management methods, such as cultivation or fallowing, have led to significant changes in soil fertility and hence, crop yield. Such changes may have stemmed from changes in soil microbial communities and associated biogeochemical processes. This phenomenon is particularly true in organic-poor soil in the Loess Plateau of China. In this study, we examined three existing soil management regimes as part of a 10-year field experiment and evaluated their effects on fungal and bacterial community structures by performing high-throughput 454 pyrosequencing. These management regimes were (i) fertilized winter wheat (Triticum aestivum L.) (FW), (ii) continuous natural fallow with weeds but without crop grown (NF), and (iii) continuous bare fallow without weeds or crop grown (BF). After 10 years, soil organic carbon (SOC), microbial biomass carbon (MBC), and available potassium (K) concentrations were highest in NF. Soil N behaved differently, with BF obtaining the highest nitrate nitrogen (N). Meanwhile, slight differences in total N (TN) were observed among FW, NF, and BF. Available phosphorus (P) was highest and available K was lowest in FW. Microbial communities were dominated by Ascomycota (59.1% of fungal sequences), and Acidobacteria, Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria (75.7% of bacterial sequences) in FW, NF and BF at the phylum level. Soil management regimes did not affect the fungal and bacterial richness and diversity but significantly modified their community compositions. Compared with FW, the abundances of Ascomycota (fungi phylum) and Alternaria, Gibberella, and Emericella (fungi genus) were increased by NF, whereas the values of Chaetomium, Humicola, and Cryptococcus (fungi genus) were decreased by BF. The abundances of Verrucomicrobia (bacteria phylum), and Steroidobacter (bacteria genus) were increased by NF, and Bacteroides (bacteria genus) was increased by BF. Canonical correspondence analysis showed that SOC, available P, and TN might be the key factors in community formation. Therefore, the decadal absence of plants (BF) affected soil fertility by increased available K and nitrate N, whileas natural fallow (NF) affected soil fertility by increased SOC, available K, and MBC, and they all changed fungal and bacterial community compositions.
[Mh] Termos MeSH primário: Microbiologia do Solo
Solo/classificação
Triticum
[Mh] Termos MeSH secundário: Alternaria/isolamento & purificação
Ascomicetos/isolamento & purificação
Biomassa
Carbono
Chaetomium/isolamento & purificação
China
Cryptococcus/isolamento & purificação
Emericella/isolamento & purificação
Gibberella/isolamento & purificação
Nitrogênio/análise
Potássio/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Soil); 7440-44-0 (Carbon); N762921K75 (Nitrogen); RWP5GA015D (Potassium)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170908
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184223


  3 / 2354 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28829788
[Au] Autor:Cassim N; Coetzee LM; Schnippel K; Glencross DK
[Ad] Endereço:National Health Laboratory Service (NHLS), National Priority Programmes, Johannesburg, South Africa.
[Ti] Título:Estimating the cost-per-result of a national reflexed Cryptococcal antigenaemia screening program: Forecasting the impact of potential HIV guideline changes and treatment goals.
[So] Source:PLoS One;12(8):e0182154, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: During 2016, the National Health Laboratory Service (NHLS) introduced laboratory-based reflexed Cryptococcal antigen (CrAg) screening to detect early Cryptococcal disease in immunosuppressed HIV+ patients with a confirmed CD4 count of 100 cells/µl or less. OBJECTIVE: The aim of this study was to assess cost-per-result of a national screening program across different tiers of laboratory service, with variable daily CrAg test volumes. The impact of potential ART treatment guideline and treatment target changes on CrAg volumes, platform choice and laboratory workflow are considered. METHODS: CD4 data (with counts < = 100 cells/µl) from the fiscal year 2015/16 were extracted from the NHLS Corporate Date Warehouse and used to project anticipated daily CrAg testing volumes with appropriately-matched CrAg testing platforms allocated at each of 52 NHLS CD4 laboratories. A cost-per-result was calculated for four scenarios, including the existing service status quo (Scenario-I), and three other settings (as Scenarios II-IV) which were based on information from recent antiretroviral (ART) guidelines, District Health Information System (DHIS) data and UNAIDS 90/90/90 HIV/AIDS treatment targets. Scenario-II forecast CD4 testing offered only to new ART initiates recorded at DHIS. Scenario-III projected all patients notified as HIV+, but not yet on ART (recorded at DHIS) and Scenario-IV forecast CrAg screening in 90% of estimated HIV+ patients across South Africa (also DHIS). Stata was used to assess daily CrAg volumes at the 5th, 10th, 25th, 50th, 75th, 90th and 95th percentiles across 52 CD4-laboratories. Daily volumes were used to determine technical effort/ operator staff costs (% full time equivalent) and cost-per-result for all scenarios. RESULTS: Daily volumes ranged between 3 and 64 samples for Scenario-I at the 5th and 95th percentile. Similarly, daily volumes ranges of 1-12, 2-45 and 5-100 CrAg-directed samples were noted for Scenario's II, III and IV respectively. A cut-off of 30 CrAg tests per day defined use of either LFA or EIA platform. LFA cost-per-result ranged from $8.24 to $5.44 and EIA cost-per-result between $5.58 and $4.88 across the range of test volumes. The technical effort across scenarios ranged from 3.2-27.6% depending on test volumes and platform used. CONCLUSION: The study reported the impact of programmatic testing requirements on varying CrAg test volumes that subsequently influenced choice of testing platform, laboratory workflow and cost-per-result. A novel percentiles approach is described that enables an overview of the cost-per-result across a national program. This approach facilitates cross-subsidisation of more expensive lower volume sites with cost-efficient, more centralized higher volume laboratories, mitigating against the risk of costing tests at a single site.
[Mh] Termos MeSH primário: Antígenos de Fungos/análise
Cryptococcus/imunologia
Guias como Assunto
Infecções por HIV/complicações
Meningite Criptocócica/diagnóstico
[Mh] Termos MeSH secundário: Seres Humanos
Meningite Criptocócica/complicações
Meningite Criptocócica/economia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Fungal)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170823
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182154


  4 / 2354 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28800596
[Au] Autor:Sun S; Yadav V; Billmyre RB; Cuomo CA; Nowrousian M; Wang L; Souciet JL; Boekhout T; Porcel B; Wincker P; Granek JA; Sanyal K; Heitman J
[Ad] Endereço:Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina, United States of America.
[Ti] Título:Fungal genome and mating system transitions facilitated by chromosomal translocations involving intercentromeric recombination.
[So] Source:PLoS Biol;15(8):e2002527, 2017 Aug.
[Is] ISSN:1545-7885
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Species within the human pathogenic Cryptococcus species complex are major threats to public health, causing approximately 1 million annual infections globally. Cryptococcus amylolentus is the most closely known related species of the pathogenic Cryptococcus species complex, and it is non-pathogenic. Additionally, while pathogenic Cryptococcus species have bipolar mating systems with a single large mating type (MAT) locus that represents a derived state in Basidiomycetes, C. amylolentus has a tetrapolar mating system with 2 MAT loci (P/R and HD) located on different chromosomes. Thus, studying C. amylolentus will shed light on the transition from tetrapolar to bipolar mating systems in the pathogenic Cryptococcus species, as well as its possible link with the origin and evolution of pathogenesis. In this study, we sequenced, assembled, and annotated the genomes of 2 C. amylolentus isolates, CBS6039 and CBS6273, which are sexual and interfertile. Genome comparison between the 2 C. amylolentus isolates identified the boundaries and the complete gene contents of the P/R and HD MAT loci. Bioinformatic and chromatin immunoprecipitation sequencing (ChIP-seq) analyses revealed that, similar to those of the pathogenic Cryptococcus species, C. amylolentus has regional centromeres (CENs) that are enriched with species-specific transposable and repetitive DNA elements. Additionally, we found that while neither the P/R nor the HD locus is physically closely linked to its centromere in C. amylolentus, and the regions between the MAT loci and their respective centromeres show overall synteny between the 2 genomes, both MAT loci exhibit genetic linkage to their respective centromere during meiosis, suggesting the presence of recombinational suppressors and/or epistatic gene interactions in the MAT-CEN intervening regions. Furthermore, genomic comparisons between C. amylolentus and related pathogenic Cryptococcus species provide evidence that multiple chromosomal rearrangements mediated by intercentromeric recombination have occurred during descent of the 2 lineages from their common ancestor. Taken together, our findings support a model in which the evolution of the bipolar mating system was initiated by an ectopic recombination event mediated by similar repetitive centromeric DNA elements shared between chromosomes. This translocation brought the P/R and HD loci onto the same chromosome, and further chromosomal rearrangements then resulted in the 2 MAT loci becoming physically linked and eventually fusing to form the single contiguous MAT locus that is now extant in the pathogenic Cryptococcus species.
[Mh] Termos MeSH primário: Cryptococcus/citologia
Cryptococcus/genética
Genes Fúngicos Tipo Acasalamento
Genoma Fúngico
Meiose
Translocação Genética
[Mh] Termos MeSH secundário: Imunoprecipitação da Cromatina
Biologia Computacional
Troca Genética
Cryptococcus/crescimento & desenvolvimento
Cryptococcus/fisiologia
Cryptococcus neoformans/citologia
Cryptococcus neoformans/genética
Cryptococcus neoformans/fisiologia
Epistasia Genética
Evolução Molecular
Ligação Genética
Loci Gênicos
Estruturas Genéticas
Desequilíbrio de Ligação
Anotação de Sequência Molecular
Recombinação Genética
Análise de Sequência de RNA
Especificidade da Espécie
Sintenia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171014
[Lr] Data última revisão:
171014
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170812
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pbio.2002527


  5 / 2354 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28763969
[Au] Autor:Bierla K; Suzuki N; Ogra Y; Szpunar J; Lobinski R
[Ad] Endereço:CNRS/UPPA, Institute for Analytical Sciences and Physical Chemistry for the Environment and Materials (IPREM), UMR 5254, Hélioparc, F-64053 Pau, France.
[Ti] Título:Identification and determination of selenohomolanthionine - The major selenium compound in Torula yeast.
[So] Source:Food Chem;237:1196-1201, 2017 Dec 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Torula yeast (Candida utilis) was found to metabolize selenium in a totally different way to Brewer's yeast (S. cerevisiae) leading to the biosynthesis of selenohomolanthionine (SeHLan), a major selenium compound accounting for 60-80% of the total selenium. The identity of SeHLan was confirmed by retention time matching in hydrophilic ion interaction chromatography (HILIC) with inductively coupled plasma mass spectrometric detection (ICP MS) using a custom synthesized standard molecule and by HILIC - Orbitrap MS and MS-MS fragmentation. Selenohomolanthionine escapes the current assays for the organic character of Se-rich yeast based on the protein-bound selenomethionine determination. A HILIC - ICP MS method was developed for the quantitative determination of selenohomolanthionine in yeast supplements with a detection limit of 146ng/g.
[Mh] Termos MeSH primário: Cryptococcus/química
[Mh] Termos MeSH secundário: Cromatografia Líquida de Alta Pressão
Homocisteína/análogos & derivados
Espectrometria de Massas
Compostos Organosselênicos
Selênio
Compostos de Selênio
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (4,4'-selenobis(2-aminobutanoic acid)); 0 (Organoselenium Compounds); 0 (Selenium Compounds); 0LVT1QZ0BA (Homocysteine); H6241UJ22B (Selenium)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170803
[St] Status:MEDLINE


  6 / 2354 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28750078
[Au] Autor:Kwizera R; Akampurira A; Williams D; Boulware DR; Meya DB; ASTRO-CM Study Team
[Ad] Endereço:Infectious Diseases Institute, College of Health Sciences, Makerere University, Kampala, Uganda.
[Ti] Título:Acridine orange fluorescent microscopy is more sensitive than India ink light microscopy in the rapid detection of cryptococcosis among CrAg positive HIV patients.
[So] Source:PLoS One;12(7):e0182108, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: India ink microscopy on cerebrospinal fluid is still utilized in resource limited settings for the diagnosis of cryptococcal meningitis despite its poor sensitivity. We hypothesized that staining fungal nucleic acids with fluorescent dyes instead of the capsule with India ink might improve sensitivity for the diagnosis of cryptococcal meningitis. METHODS: We enrolled 96 HIV-infected participants with cryptococcal meningitis who provided 194 CSF specimens at serial time points in Kampala, Uganda. Cryptococcosis was diagnosed by cerebrospinal fluid (CSF) cryptococcal antigen (CrAg) test and only positive samples were included. We stained CSF with India ink and acridine orange. We cultured the same samples on standard fungal media. We compared acridine orange to CrAg, India ink and CSF culture. RESULTS: Acridine orange was more sensitive (96%) than India ink (79%) with reference to CSF CrAg. Acridine orange and India ink had a statistically significant difference (P<0.001) with a 25% correlation for detection of Cryptococcus yeasts. India ink had more negative results (22%) than acridine orange (4%). The sensitivity for India ink increased (86%) while that of acridine orange did not change (97%) when compared to CSF culture. However, both India ink and acridine orange had poor predictive values with reference to culture. CONCLUSION: Acridine orange is a better alternative to India ink in the rapid detection of cryptococcosis among CrAg positive HIV patients.
[Mh] Termos MeSH primário: Laranja Acridina/química
Antígenos de Fungos/imunologia
Carbono/química
Criptococose/diagnóstico
Cryptococcus/imunologia
Infecções por HIV/complicações
Infecções por HIV/microbiologia
Microscopia de Fluorescência/métodos
[Mh] Termos MeSH secundário: Adulto
Criptococose/microbiologia
Cryptococcus/citologia
Feminino
Seres Humanos
Masculino
Meningite Criptocócica/diagnóstico
Meningite Criptocócica/microbiologia
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Fungal); 0 (chinese ink); 7440-44-0 (Carbon); F30N4O6XVV (Acridine Orange)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170728
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182108


  7 / 2354 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28609752
[Au] Autor:Liu J; Yuan M; Liu JN; Huang XF
[Ad] Endereço:College of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Ministry of Education Key Laboratory of Yangtze River Water Environment, Collaborative Innovation Center for Regional Environmental Quality, Tongji University, Shanghai 200092, China.
[Ti] Título:Bioconversion of mixed volatile fatty acids into microbial lipids by Cryptococcus curvatus ATCC 20509.
[So] Source:Bioresour Technol;241:645-651, 2017 Oct.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The oleaginous yeast Cryptococcus curvatus ATCC 20509 can use 5-40g/L of acetic, propionic, or butyric acid as sole carbon source to produce lipids. High concentrations (30g/L) of mixed volatile fatty acids (VFAs) were used to cultivate C. curvatus to explore the effects of different ratios of mixed VFAs on lipid production and composition. When mixed VFAs (VFA ratio was 15:5:10) were used as carbon sources, the highest cell mass and lipid concentration were 8.68g/L and 4.93g/L, respectively, which were significantly higher than those when 30g/L of acetic acid was used as sole carbon source. The highest content and yield of odd-numbered fatty acids were 45.1% (VFA ratio was 0:15:15) and 1.62g/L (VFA ratio was 5:15:10), respectively. These results indicate that adjusting the composition ratios of mixed VFAs effectively improves microbial lipid synthesis and the yield of odd-numbered fatty acids.
[Mh] Termos MeSH primário: Cryptococcus
Ácidos Graxos Voláteis
[Mh] Termos MeSH secundário: Ácidos Graxos
Lipídeos
Leveduras
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fatty Acids); 0 (Fatty Acids, Volatile); 0 (Lipids)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170614
[St] Status:MEDLINE


  8 / 2354 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28535491
[Au] Autor:Liu J; Huang X; Chen R; Yuan M; Liu J
[Ad] Endereço:College of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Ministry of Education Key Laboratory of Yangtze River Water Environment, The Collaborative Innovation Center For Regional Environmental Quality, Tongji University, Shanghai 200092, China.
[Ti] Título:Efficient bioconversion of high-content volatile fatty acids into microbial lipids by Cryptococcus curvatus ATCC 20509.
[So] Source:Bioresour Technol;239:394-401, 2017 Sep.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The lower utilization ratios of high-content VFAs resulted in less lipid yield by the oleaginous yeast Cryptococcus curvatus ATCC 20509. In this work, increasing the inoculation concentration to OD600=5.1 improved the acetic acid utilization ratio (99.8%) and lipids production (4.63g/L) in mediums with 30g/L of acetic acid. For the higher acetic acid concentration (40g/L), increasing the nitrogen to carbon ratio (0.033) and raising the initial pH (pH=8) was superior over improvement in the inoculate, with the lipid production increased from 1.08g/L to 6.49g/L. Subsequently, mixed VFAs at concentrations of 30g/L and 40g/L were used as the carbon source to simulate waste-derived VFAs. High lipid production (4.82 and 7.45g/L, respectively) was correspondingly achieved with similar high lipid yield (0.187g/g). This study provides an effective strategy to enhance the bioconversion of high-content VFAs into microbial lipids.
[Mh] Termos MeSH primário: Cryptococcus
Ácidos Graxos Voláteis
Lipídeos
[Mh] Termos MeSH secundário: Carbono
Ácidos Graxos
Nitrogênio
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fatty Acids); 0 (Fatty Acids, Volatile); 0 (Lipids); 7440-44-0 (Carbon); N762921K75 (Nitrogen)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170524
[St] Status:MEDLINE


  9 / 2354 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28444776
[Au] Autor:Kong Q; Du X; Huang S; Yang R; Zhang C; Shen Y; Liu W; Sang H
[Ad] Endereço:Southern Medical University, Guangzhou, China.
[Ti] Título:In vitro effects of ambroxol on Cryptococcus adherence, planktonic cells, and biofilms.
[So] Source:APMIS;125(7):634-640, 2017 Jul.
[Is] ISSN:1600-0463
[Cp] País de publicação:Denmark
[La] Idioma:eng
[Ab] Resumo:The antifungal effects of ambroxol (Amb; the metabolite VIII of bromhexine) against Cryptococcus planktonic cells and mature biofilms were investigated in this study. Amb showed antifungal activity against planktonic cells and mature biofilms. Disk diffusion test similarly showed antifungal profile for planktonic cells. Furthermore, Amb was found to be synergetic with fluconazole against planktonic cells and reduced the adherence of cells to polystyrene. Our results suggest that Amb can inhibit cryptococcal cells and biofilms, indicating its potential role in the prevention and treatment of cryptococcosis.
[Mh] Termos MeSH primário: Ambroxol/farmacologia
Antifúngicos/farmacologia
Biofilmes/efeitos dos fármacos
Adesão Celular/efeitos dos fármacos
Cryptococcus/efeitos dos fármacos
[Mh] Termos MeSH secundário: Adulto
Idoso
Criptococose/microbiologia
Cryptococcus/isolamento & purificação
Cryptococcus/fisiologia
Sinergismo Farmacológico
Feminino
Fluconazol/farmacologia
Seres Humanos
Masculino
Testes de Sensibilidade Microbiana
Meia-Idade
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 200168S0CL (Ambroxol); 8VZV102JFY (Fluconazole)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170717
[Lr] Data última revisão:
170717
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170427
[St] Status:MEDLINE
[do] DOI:10.1111/apm.12698


  10 / 2354 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28411053
[Au] Autor:Deeba F; Pruthi V; Negi YS
[Ad] Endereço:Department of Polymer and Process Engineering, Indian Institute of Technology Roorkee (IIT R), Saharanpur Campus, Saharanpur 247001, India.
[Ti] Título:Fostering triacylglycerol accumulation in novel oleaginous yeast Cryptococcus psychrotolerans IITRFD utilizing groundnut shell for improved biodiesel production.
[So] Source:Bioresour Technol;242:113-120, 2017 Oct.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The investigation was carried out to examine the potential of triacylglycerol (TAG) accumulation by novel oleaginous yeast isolate Cryptococcus psychrotolerans IITRFD on utilizing groundnut shell acid hydrolysate (GSH) as cost-effective medium. The maximum biomass productivity and lipid productivity of 0.095±0.008g/L/h and 0.044±0.005g/L/h, respectively with lipid content 46% was recorded on GSH. Fatty acid methyl ester (FAME) profile obtained by GC-MS analysis revealed oleic acid (37.8%), palmitic (29.4%) and linoleic (32.8%) as major fatty acids representing balance between oxidative stability (OS) and cold flow filter properties (CFFP) for improved biodiesel quality. The biodiesel property calculated were correlated well with the fuel standards limits of ASTM D6751, EN 14214 and IS 15607. The present findings raise the possibility of using agricultural waste groundnut shell as a substrate for production of biodiesel by novel oleaginous yeast isolate C. psychrotolerans IITRFD.
[Mh] Termos MeSH primário: Biocombustíveis
Cryptococcus
Triglicerídeos
[Mh] Termos MeSH secundário: Biomassa
Ácidos Graxos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biofuels); 0 (Fatty Acids); 0 (Triglycerides)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170416
[St] Status:MEDLINE



página 1 de 236 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde