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  1 / 19 MEDLINE  
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[PMID]:26725242
[Au] Autor:Liu P; Chen K; Li G; Yang X; Long CA
[Ad] Endereço:Key Laboratory of Pomology, Anhui Agricultural University, Hefei, 230036, P. R. China. puliuchina@gmail.com.
[Ti] Título:Comparative transcriptional profiling of orange fruit in response to the biocontrol yeast Kloeckera apiculata and its active compounds.
[So] Source:BMC Genomics;17:17, 2016 Jan 04.
[Is] ISSN:1471-2164
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The yeast Kloeckera apiculata strain 34-9 is an antagonist that shows biological control activity against the postharvest fungal pathogens of citrus. An antifungal compound, 2-phenylethanol (PEA), has been identified from the extract of K. apiculata. To better understand the molecular processes underlying the response of citrus fruit tissue to K. apiculata, the extract and PEA, microarray analyses were performed on navel oranges using an Affymetrix Citrus GeneChip. RESULTS: As many as 801, 339 and 608 differentially expressed genes (DEGs) were identified after the application of K. apiculata, the extract and PEA, respectively. In general, K. apiculata induced the expression of defence-related genes. In addition to chitinase and ß-1,3-glucanase, genes involved in ethylene (ET), jasmonic acid (JA), calcium signalling, MAPK signalling and phenylalanine metabolism were induced. In contrast, monodehydroascorbate reductase, superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) and carotenoid biosynthesis genes were down-regulated. The expression profiles for the extract- and PEA-treated samples were similar to that found for yeast (sharing 57.4 % DEGs), with a significant increase in the transcript levels of defence-related genes. CONCLUSION: This study provides a global picture of the gene expression changes in navel oranges after the application of the antagonist yeast K. apiculata, its extract and PEA. The interpretation of the DEGs revealed new insight into the molecular processes that regulate the defence responses in orange tissue.
[Mh] Termos MeSH primário: Sinalização do Cálcio/genética
Citrus sinensis/genética
Frutas/genética
Kloeckera/efeitos dos fármacos
[Mh] Termos MeSH secundário: Citrus sinensis/crescimento & desenvolvimento
Citrus sinensis/microbiologia
Ciclopentanos/metabolismo
Etilenos/metabolismo
Frutas/crescimento & desenvolvimento
Perfilação da Expressão Gênica
Regulação da Expressão Gênica de Plantas
Kloeckera/patogenicidade
Quinases de Proteína Quinase Ativadas por Mitógeno/genética
Oxilipinas/metabolismo
Fenilalanina/metabolismo
Álcool Feniletílico/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cyclopentanes); 0 (Ethylenes); 0 (Oxylipins); 47E5O17Y3R (Phenylalanine); 6RI5N05OWW (jasmonic acid); 91GW059KN7 (ethylene); EC 2.7.12.2 (Mitogen-Activated Protein Kinase Kinases); ML9LGA7468 (Phenylethyl Alcohol)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160105
[St] Status:MEDLINE
[do] DOI:10.1186/s12864-015-2333-3


  2 / 19 MEDLINE  
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[PMID]:26108494
[Au] Autor:González-Robles IW; Estarrón-Espinosa M; Díaz-Montaño DM
[Ad] Endereço:Unidad de Biotecnología Industrial, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C., Av. Normalistas 800, Col. Colinas de la Normal, 44270, Guadalajara, Jalisco, Mexico, stxig@nottingham.ac.uk.
[Ti] Título:Fermentative capabilities and volatile compounds produced by Kloeckera/Hanseniaspora and Saccharomyces yeast strains in pure and mixed cultures during Agave tequilana juice fermentation.
[So] Source:Antonie Van Leeuwenhoek;108(3):525-36, 2015 Sep.
[Is] ISSN:1572-9699
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The fermentative and aromatic capabilities of Kloeckera africana/Hanseniaspora vineae K1, K. apiculata/H. uvarum K2, and Saccharomyces cerevisiae S1 and S2 were studied in pure and mixed culture fermentations using Agave tequila juice as the culture medium. In pure and mixed cultures, Kloeckera/Hanseniaspora strains showed limited growth and sugar consumption, as well as low ethanol yield and productivity, compared to S. cerevisiae, which yielded more biomass, ethanol and viable cell concentrations. In pure and mixed cultures, S. cerevisiae presented a similar behaviour reaching high biomass production, completely consuming the sugar, leading to high ethanol production. Furthermore, the presence of S. cerevisiae strains in the mixed cultures promoted the production of higher alcohols, acetaldehyde and ethyl esters, whereas Kloeckera/Hanseniaspora strains stimulated the production of ethyl acetate and 2-phenyl ethyl acetate compounds.
[Mh] Termos MeSH primário: Agave/metabolismo
Bebidas Alcoólicas/microbiologia
Kloeckera/metabolismo
Saccharomyces/metabolismo
Compostos Orgânicos Voláteis/análise
[Mh] Termos MeSH secundário: Fermentação
Kloeckera/crescimento & desenvolvimento
Consórcios Microbianos
Saccharomyces/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Volatile Organic Compounds)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:150805
[Lr] Data última revisão:
150805
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150626
[St] Status:MEDLINE
[do] DOI:10.1007/s10482-015-0506-3


  3 / 19 MEDLINE  
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[PMID]:25230758
[Au] Autor:Liu P; Cheng Y; Yang M; Liu Y; Chen K; Long CA; Deng X
[Ti] Título:Mechanisms of action for 2-phenylethanol isolated from Kloeckera apiculata in control of Penicillium molds of citrus fruits.
[So] Source:BMC Microbiol;14:242, 2014 Sep 19.
[Is] ISSN:1471-2180
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Green and blue mold decay, caused by Penicillium digitatum and P. italicum, respectively, are important postharvest diseases of citrus. Biocontrol by microbes is an alternative to synthetic fungicide application. In this study, the antagonistic yeast strain Kloeckera apiculata 34-9 was used to investigate the action mechanisms involved in the biocontrol of postharvest diseases. RESULTS: An antifungal substance, 2-phenylethanol (PEA), was isolated from K. apiculata and demonstrated to have antimicrobial activity against selected phytopathogenic fungi. Experiments on P. italicum cells identified the mitochondria and the nucleus as particularly sensitive to inhibition. Regulation of P. italicum gene expression was investigated using RNA-Seq. PEA up-regulated genes involved with the peroxisome, regulation of autophagy, phosphatidylinositol signaling system, protein processing in endoplasmic reticulum, fatty acid metabolism, and inhibited ribosome, RNA polymerase, DNA replication, amino acid biosynthesis, aminoacyl-tRNA biosynthesis and cell cycle. Inhibitory responses revealed by RNA-Seq suggest that PEA might compete for attachment on the active site of phenylalanyl-tRNA synthetase (PheRS). CONCLUSION: This study provided new insight on the mode of action of biocontrol yeast agents in controlling postharvest pathogenic fungi.
[Mh] Termos MeSH primário: Citrus/microbiologia
Kloeckera/química
Penicillium/efeitos dos fármacos
Álcool Feniletílico/química
Álcool Feniletílico/farmacologia
[Mh] Termos MeSH secundário: Antifúngicos/química
Antifúngicos/farmacologia
Núcleo Celular/efeitos dos fármacos
Conservação de Alimentos/métodos
Fungos/efeitos dos fármacos
Fungicidas Industriais/química
Fungicidas Industriais/farmacologia
Expressão Gênica/efeitos dos fármacos
Mitocôndrias/efeitos dos fármacos
Doenças das Plantas/microbiologia
Transdução de Sinais/efeitos dos fármacos
Leveduras/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Fungicides, Industrial); ML9LGA7468 (Phenylethyl Alcohol)
[Em] Mês de entrada:1509
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140919
[St] Status:MEDLINE
[do] DOI:10.1186/s12866-014-0242-2


  4 / 19 MEDLINE  
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[PMID]:25123413
[Au] Autor:Ale CE; Bru E; Strasser de Saad AM; Pasteris SE
[Ad] Endereço:Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICET-UNT, and Instituto de Biología "Dr. Francisco D. Barbieri", Facultad de Bioquímica, Química y Farmacia, UNT, San Miguel de Tucumán, Argentina.
[Ti] Título:Effect of physicochemical factors on glycerol production by simultaneous cultures of wine micro-organisms using the response surface method.
[So] Source:J Appl Microbiol;117(5):1336-47, 2014 Nov.
[Is] ISSN:1365-2672
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:AIM: To evaluate the effect of temperature, pH and SO2 on growth and glycerol production improvement by Saccharomyces cerevisiae mc2, Kloeckera apiculata mF and Oenococcus oeni X2L using the response surface method (RSM). METHODS AND RESULTS: Multifactorial design of cultures with physicochemical factors variations was performed. The micro-organisms grew in all cultures conditions. Overall, after 6 days yeasts prevailed, especially S. cerevisiae (10(9) CFU ml(-1)), while O. oeni reached 10(7) CFU ml(-1). At initial fixed pH 5·5, metabolic behaviour of cultures showed a temperature-dependent response. Total malate consumption occurred at 26°C, 50 mg l(-1) SO2. Glucose and pentoses utilization was highly modified when varying SO2. Ethanol showed negative interaction with temperature-SO2 relationship. At low SO2, glycerol and acetate production increased when temperature enhanced. Predictive results of RSM indicate that 26°C, 60·24 mg l(-1) SO2 and pH 5·5 were the optimal conditions for glycerol and organic acids synthesis compatible with wine quality. CONCLUSIONS: We propose a predictive condition to improve the performance of mixed cultures for must fermentations. SIGNIFICANCE AND IMPACT OF THE STUDY: To optimize the culture conditions to design mixed starters containing autochthonous yeasts and O. oeni strains for winemaking and to obtain products with high glycerol content, low acidity and maintenance of regional characteristics.
[Mh] Termos MeSH primário: Glicerol/metabolismo
Vinho/microbiologia
[Mh] Termos MeSH secundário: Interpretação Estatística de Dados
Fermentação
Concentração de Íons de Hidrogênio
Kloeckera/crescimento & desenvolvimento
Kloeckera/metabolismo
Oenococcus/crescimento & desenvolvimento
Oenococcus/metabolismo
Saccharomyces cerevisiae/crescimento & desenvolvimento
Saccharomyces cerevisiae/metabolismo
Dióxido de Enxofre
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0UZA3422Q4 (Sulfur Dioxide); PDC6A3C0OX (Glycerol)
[Em] Mês de entrada:1503
[Cu] Atualização por classe:141014
[Lr] Data última revisão:
141014
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140816
[St] Status:MEDLINE
[do] DOI:10.1111/jam.12621


  5 / 19 MEDLINE  
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[PMID]:24752716
[Au] Autor:Ale CE; Farías ME; Strasser de Saad AM; Pasteris SE
[Ad] Endereço:Instituto de Microbiología, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, San Miguel de Tucumán, Argentina.
[Ti] Título:Glycerol production by Oenococcus oeni during sequential and simultaneous cultures with wine yeast strains.
[So] Source:J Basic Microbiol;54 Suppl 1:S200-9, 2014 Jul.
[Is] ISSN:1521-4028
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Growth and fermentation patterns of Saccharomyces cerevisiae, Kloeckera apiculata, and Oenococcus oeni strains cultured in grape juice medium were studied. In pure, sequential and simultaneous cultures, the strains reached the stationary growth phase between 2 and 3 days. Pure and mixed K. apiculata and S. cerevisiae cultures used mainly glucose, producing ethanol, organic acids, and 4.0 and 0.1 mM glycerol, respectively. In sequential cultures, O. oeni achieved about 1 log unit at 3 days using mainly fructose and L-malic acid. Highest sugars consumption was detected in K. apiculata supernatants, lactic acid being the major end-product. 8.0 mM glycerol was found in 6-day culture supernatants. In simultaneous cultures, total sugars and L-malic acid were used at 3 days and 98% of ethanol and glycerol were detected. This study represents the first report of the population dynamics and metabolic behavior of yeasts and O. oeni in sequential and simultaneous cultures and contributes to the selection of indigenous strains to design starter cultures for winemaking, also considering the inclusion of K. apiculata. The sequential inoculation of yeasts and O. oeni would enhance glycerol production, which confers desirable organoleptic characteristics to wines, while organic acids levels would not affect their sensory profile.
[Mh] Termos MeSH primário: Glicerol/metabolismo
Kloeckera/crescimento & desenvolvimento
Kloeckera/metabolismo
Oenococcus/crescimento & desenvolvimento
Oenococcus/metabolismo
Saccharomyces cerevisiae/crescimento & desenvolvimento
Saccharomyces cerevisiae/metabolismo
[Mh] Termos MeSH secundário: Ácidos Carboxílicos/metabolismo
Fermentação
Glucose/metabolismo
Fatores de Tempo
Vinho/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Carboxylic Acids); IY9XDZ35W2 (Glucose); PDC6A3C0OX (Glycerol)
[Em] Mês de entrada:1503
[Cu] Atualização por classe:140818
[Lr] Data última revisão:
140818
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140423
[St] Status:MEDLINE
[do] DOI:10.1002/jobm.201300934


  6 / 19 MEDLINE  
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[PMID]:24479773
[Au] Autor:Pu L; Jingfan F; Kai C; Chao-an L; Yunjiang C
[Ad] Endereço:National Center of Citrus Breeding, Key Laboratory of Horticultural Plant Biology of Ministry of Education, Huazhong Agricultural University, Wuhan, China; Key Laboratory of Pomology, Anhui Agricultural University, Hefei, China.
[Ti] Título:Phenylethanol promotes adhesion and biofilm formation of the antagonistic yeast Kloeckera apiculata for the control of blue mold on citrus.
[So] Source:FEMS Yeast Res;14(4):536-46, 2014 Jun.
[Is] ISSN:1567-1364
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The yeast Kloeckera apiculata strain 34-9 is an antagonist with biological control activity against postharvest diseases of citrus fruit. In a previous study it was demonstrated that K. apiculata produced the aromatic alcohol phenylethanol. In the present study, we found that K. apiculata was able to form biofilm on citrus fruit and embed in an extracellular matrix, which created a mechanical barrier interposed between the wound surface and pathogen. As a quorum-sensing molecule, phenylethanol can promote the formation of filaments by K. apiculata in potato dextrose agar medium, whereas on the citrus fruit, the antagonist remains as yeast after being treated with the same concentration of phenylethanol. It only induced K. apiculata to adhere and form biofilm. Following genome-wide computational and experimental identification of the possible genes associated with K. apiculata adhesion, we identified nine genes possibly involved in triggering yeast adhesion. Six of these genes were significantly induced after phenylethanol stress treatment. This study provides a new model system of the biology of the antagonist-pathogen interactions that occur in the antagonistic yeast K. apiculata for the control of blue mold on citrus caused by Penicillium italicum.
[Mh] Termos MeSH primário: Antibiose
Biofilmes/efeitos dos fármacos
Citrus/microbiologia
Conservação de Alimentos/métodos
Fungos/crescimento & desenvolvimento
Kloeckera/efeitos dos fármacos
Álcool Feniletílico/metabolismo
[Mh] Termos MeSH secundário: Biofilmes/crescimento & desenvolvimento
Adesão Celular
Perfilação da Expressão Gênica
Kloeckera/fisiologia
Controle Biológico de Vetores/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
ML9LGA7468 (Phenylethyl Alcohol)
[Em] Mês de entrada:1501
[Cu] Atualização por classe:140609
[Lr] Data última revisão:
140609
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140201
[St] Status:MEDLINE
[do] DOI:10.1111/1567-1364.12139


  7 / 19 MEDLINE  
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[PMID]:24372267
[Au] Autor:Vallejo CV; Aredes-Fernández PA; Farías ME; Rodríguez-Vaquero MJ
[Ti] Título:Biofilm inhibition of spoilage bacteria by Argentinean fruit juices with antihypertensive activity.
[So] Source:Curr Pharm Biotechnol;14(9):802-8, 2013.
[Is] ISSN:1873-4316
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Argentinean juices have been studied for their antihypertensive activity, the inhibition of bacteria biofilm formation and the effect on the viability of wine yeast. The influence of phenolic compounds on these activities was evaluated. These studies are the first step for the development of a new type of wine that includes grape must supplement with fruit juices with antihypertensive effect. All juices posses a high antihypertensive activity, higher than 50%. Strawberry juices and eureka lemon showed the highest activity, whereas clarified juices posses the lowest activity. All studied juices produce a high inhibition of bacteria biofilm formation, and the strawberry, orange and mandarin varieties not affect the growth or viability of yeast. Our results permit to conclude that it could be possible the use of these juices in a new type of wine or as a source of new antihypertensive agents for pharmaceutical industry.
[Mh] Termos MeSH primário: Inibidores da Enzima Conversora de Angiotensina/farmacologia
Anti-Hipertensivos/farmacologia
Bebidas
Conservantes de Alimentos/farmacologia
Frutas
[Mh] Termos MeSH secundário: Argentina
Bactérias/efeitos dos fármacos
Bactérias/crescimento & desenvolvimento
Biofilmes/efeitos dos fármacos
Biofilmes/crescimento & desenvolvimento
Kloeckera/efeitos dos fármacos
Kloeckera/crescimento & desenvolvimento
Testes de Sensibilidade Microbiana
Saccharomyces cerevisiae/efeitos dos fármacos
Saccharomyces cerevisiae/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Angiotensin-Converting Enzyme Inhibitors); 0 (Antihypertensive Agents); 0 (Food Preservatives)
[Em] Mês de entrada:1409
[Cu] Atualização por classe:141021
[Lr] Data última revisão:
141021
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131231
[St] Status:MEDLINE


  8 / 19 MEDLINE  
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[PMID]:24018998
[Au] Autor:Wójcik A; Kurnatowski P; Blaszkowska J
[Ad] Endereço:Department of Biology and Medical Parasitology, Medical University of Lodz, Lódz, Poland, anna.wojcik@umed.lodz.pl.
[Ti] Título:Potentially pathogenic yeasts from soil of children's recreational areas in the city of Lódz (Poland).
[So] Source:Int J Occup Med Environ Health;26(3):477-87, 2013 Jun.
[Is] ISSN:1896-494X
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: Yeasts may become potential human and animal pathogens, particularly for individuals with a depressed immune system. Their presence in the environment, especially in soil, may favour their spread into human ontocenoses. MATERIALS AND METHODS: Eighty-four soil samples obtained from 21 children's recreational sites in Lódz in autumn 2010 and spring 2011 were evaluated. The yeasts were isolated by classical microbiological methods and identified on the basis of morphological and biochemical features. RESULTS: The fungi were found in 73.8% and in 69.0% of the examined samples collected in autumn and spring, respectively. Among 97 isolates of yeasts, the species potentially pathogenic to humans and animals were Candida colliculosa, C. guilliermondii, C. humicola, C. inconspicua, C. lambica, C. lusitaniae, C. pelliculosa, C. tropicalis, Cryptococcus albidus, C. laurentii, C. neoformans, C. terreus, Kloeckera japonica, Geotrichum candidum, G. penicillatum, Rhodotorula mucilaginosa, R. glutinis, Saccharomyces cerevisiae, Sporobolomyces salmonicolor and Trichosporon cutaneum. The most frequently isolated fungi included the genus Cryptococcus (38 isolates) and two species: Rhodotorula glutinis (15), Trichosporon cutaneum (14). C. neoformans, an etiological factor of cryptococcal meningitis, was present in the sandpits of 3 kindergartens. The Candida species were identified from park playgrounds and school sports fields mainly in autumn 2010 (14 isolates), in spring 2011 - only 1 isolate. The concentration of fungal species in particular samples varied considerably, but in the majority of samples, fungi were present at concentration of up to 1×10(2) CFU/1 g of soil. CONCLUSIONS: Yeasts were present in the soil of parks, schools and kindergarten recreational areas; the fact may pose a health risk to humans, especially to children, and this type of biological pollution should be regarded as a potential public health concern.
[Mh] Termos MeSH primário: Meio Ambiente
Leveduras/isolamento & purificação
[Mh] Termos MeSH secundário: Candida/isolamento & purificação
Criança
Cidades
Cryptococcus/isolamento & purificação
Geotrichum/isolamento & purificação
Seres Humanos
Kloeckera/isolamento & purificação
Polônia
Recreação
Rhodotorula/isolamento & purificação
Saccharomyces/isolamento & purificação
Instituições Acadêmicas
Estações do Ano
Trichosporon/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1406
[Cu] Atualização por classe:130910
[Lr] Data última revisão:
130910
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130911
[St] Status:MEDLINE
[do] DOI:10.2478/s13382-013-0118-y


  9 / 19 MEDLINE  
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[PMID]:23223175
[Au] Autor:Cubaiu L; Abbas H; Dobson AD; Budroni M; Migheli Q
[Ad] Endereço:Dipartimento di Agraria, Università degli Studi di Sassari, Viale Italia 39, Sassari I-07100, Italy. lcubaiu@uniss.it
[Ti] Título:A Saccharomyces cerevisiae wine strain inhibits growth and decreases Ochratoxin A biosynthesis by Aspergillus carbonarius and Aspergillus ochraceus.
[So] Source:Toxins (Basel);4(12):1468-81, 2012 Dec 10.
[Is] ISSN:2072-6651
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to select wine yeast strains as biocontrol agents against fungal contaminants responsible for the accumulation of ochratoxin A (OTA) in grape and wine and to dissect the mechanism of OTA detoxification by a Saccharomyces cerevisiae strain (DISAABA1182), which had previously been reported to reduce OTA in a synthetic must. All of the yeast strains tested displayed an ability to inhibit the growth of Aspergillus carbonarius both in vivo and in vitro and addition of culture filtrates from the tested isolates led to complete inhibition of OTA production. S. cerevisiae DISAABA1182 was selected and further tested for its capacity to inhibit OTA production and pks (polyketide synthase) transcription in A. carbonarius and Aspergillus ochraceus in vitro. In order to dissect the mechanism of OTA detoxification, each of these two fungi was co-cultured with living yeast cells exposed to yeast crude or to autoclaved supernatant: S. cerevisiae DISAABA1182 was found to inhibit mycelial growth and OTA production in both Aspergilli when co-cultured in the OTA-inducing YES medium. Moreover, a decrease in pks transcription was observed in the presence of living cells of S. cerevisiae DISAABA1182 or its supernatant, while no effects were observed on transcription of either of the constitutively expressed calmodulin and ß-tubulin genes. This suggests that transcriptional regulation of OTA biosynthetic genes takes place during the interaction between DISAABA1182 and OTA-producing Aspergilli.
[Mh] Termos MeSH primário: Aspergillus/metabolismo
Carcinógenos/metabolismo
Ocratoxinas/metabolismo
Saccharomyces cerevisiae
Vinho/microbiologia
[Mh] Termos MeSH secundário: Aspergillus/crescimento & desenvolvimento
Agentes de Controle Biológico
Kloeckera
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Biological Control Agents); 0 (Carcinogens); 0 (Ochratoxins)
[Em] Mês de entrada:1305
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:121211
[St] Status:MEDLINE
[do] DOI:10.3390/toxins4121468


  10 / 19 MEDLINE  
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[PMID]:21841004
[Au] Autor:Chang CF; Huang LY; Chen SF; Lee CF
[Ad] Endereço:Department of Applied Science, National Hsinchu University of Education, Hsinchu 30014, Taiwan, ROC.
[Ti] Título:Kloeckera taiwanica sp. nov., an ascomycetous apiculate yeast species isolated from mushroom fruiting bodies.
[So] Source:Int J Syst Evol Microbiol;62(Pt 6):1434-7, 2012 Jun.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Three apiculate yeast strains, EJ7M09(T), GJ5M15 and GJ15M04, isolated from mushrooms in Taiwan were found to represent a novel species of the genus Kloeckera. The phylogenetically closest relative of this novel species is Hanseniaspora occidentalis, but the type strain of H. occidentalis differed by 4.6 % divergence (25 substitutions; 5 gaps) in the sequence of the D1/D2 domain of the large subunit rRNA gene. This difference clearly suggests that the three strains represent a distinct species. As none of the strains that were examined in this study produced ascospores or exhibited conjugation on common sporulation medium either alone or in a pairwise mixture, this species could be considered as an anamorphic member of the genus Hanseniaspora, and a novel species, Kloeckera taiwanica sp. nov., is proposed, with EJ7M09(T) ( = BCRC 23182(T) = CBS 11434(T)) as the type strain.
[Mh] Termos MeSH primário: Carpóforos/química
Kloeckera/classificação
Kloeckera/isolamento & purificação
[Mh] Termos MeSH secundário: Agaricales/química
DNA Fúngico/genética
Kloeckera/genética
Dados de Sequência Molecular
Técnicas de Tipagem Micológica
Filogenia
RNA Ribossômico 16S/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Fungal); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1208
[Cu] Atualização por classe:120604
[Lr] Data última revisão:
120604
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110816
[St] Status:MEDLINE
[do] DOI:10.1099/ijs.0.034231-0



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