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seleciona para imprimir Fotocópia Colombo, Arnaldo Lopes Camargo, Zoilo Pires de Texto completo SciELO Brasil

[PMID]: 28746570 [Au] Autor: Shikanai-Yasuda MA; Mendes RP; Colombo AL; Queiroz-Telles F; Kono ASG; Paniago AMM; Nathan A; Valle ACFD; Bagagli E; Benard G; Ferreira MS; Teixeira MM; Silva-Vergara ML; Pereira RM; Cavalcante RS; Hahn R; Durlacher RR; Khoury Z; Camargo ZP; Moretti ML; Martinez R [Ad] Endereço: Departamento de Moléstias Infecciosas e Parasitárias, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brasil. [Ti] Título: Brazilian guidelines for the clinical management of paracoccidioidomycosis. [So] Source: Rev Soc Bras Med Trop;50(5):715-740, 2017 Sep-Oct. [Is] ISSN: 1678-9849 [Cp] País de publicação: Brazil [La] Idioma: eng [Ab] Resumo: Paracoccidioidomycosis is a systemic fungal disease occurring in Latin America that is associated with rural environments and agricultural activities. However, the incidence and prevalence of paracoccidiodomycosis is underestimated because of the lack of compulsory notification. If paracoccidiodomycosis is not diagnosed and treated early and adequately, the endemic fungal infection could result in serious sequelae. While the Paracoccidioides brasiliensis ( P. brasiliensis ) complex has been known to be the causal agent of paracoccidiodomycosis, a new species, Paracoccidioides lutzii ( P. lutzii ), has been reported in Rondônia, where the disease has reached epidemic levels, and in the Central West and Pará. Accurate diagnoses and availability of antigens that are reactive with the patients' sera remain significant challenges. Therefore, the present guidelines aims to update the first Brazilian consensus on paracoccidioidomycosis by providing evidence-based recommendations for bedside patient management. This consensus summarizes etiological, ecoepidemiological, molecular epidemiological, and immunopathological data, with emphasis on clinical, microbiological, and serological diagnosis and management of clinical forms and sequelae, as well as in patients with comorbidities and immunosuppression. The consensus also includes discussion of outpatient treatments, severe disease forms, disease prevalence among special populations and resource-poor settings, a brief review of prevention and control measures, current challenges and recommendations. [Mh] Termos MeSH primário: Antifúngicos/uso terapêutico Gerenciamento Clínico Paracoccidioidomicose/tratamento farmacológico Paracoccidioidomicose/patologia [Mh] Termos MeSH secundário: Brasil Consenso Diagnóstico Diferencial Seres Humanos Itraconazol/uso terapêutico América Latina Paracoccidioides [Pt] Tipo de publicação: JOURNAL ARTICLE; PRACTICE GUIDELINE [Nm] Nome de substância: 0 (Antifungal Agents); 304NUG5GF4 (Itraconazole) [Em] Mês de entrada: 1712 [Cu] Atualização por classe: 171218 [Lr] Data última revisão: 171218 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170727 [St] Status: MEDLINE

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[PMID]: 28938005 [Au] Autor: Holanda RA; Muñoz JE; Dias LS; Silva LBR; Santos JRA; Pagliari S; Vieira ÉLM; Paixão TA; Taborda CP; Santos DA; Bruña-Romero O [Ad] Endereço: Departamento de Microbiologia, Universidade Federal de Minas Gerais, Minas Gerais, Brazil. [Ti] Título: Recombinant vaccines of a CD4+ T-cell epitope promote efficient control of Paracoccidioides brasiliensis burden by restraining primary organ infection. [So] Source: PLoS Negl Trop Dis;11(9):e0005927, 2017 Sep. [Is] ISSN: 1935-2735 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Paracoccidioidomycosis (PCM) is an infectious disease endemic to South America, caused by the thermally dimorphic fungi Paracoccidioides. Currently, there is no effective human vaccine that can be used in prophylactic or therapeutic regimes. We tested the hypothesis that the immunogenicity of the immunodominant CD4+ T-cell epitope (P10) of Paracoccidioides brasiliensis gp43 antigen might be significantly enhanced by using a hepatitis B virus-derived particle (VLP) as an antigen carrier. This chimera was administered to mice as a (His)6-purified protein (rPbT) or a replication-deficient human type 5 adenoviral vector (rAdPbT) in an immunoprophylaxis assay. The highly virulent Pb18 yeast strain was used to challenge our vaccine candidates. Fungal challenge evoked robust P10-specific memory CD4+ T cells secreting protective Th-1 cytokines in most groups of immunized mice. Furthermore, the highest level of fungal burden control was achieved when rAdPbT was inoculated in a homologous prime-boost regimen, with 10-fold less CFU recovering than in non-vaccinated mice. Systemic Pb18 spreading was only prevented when rAdPbT was previously inoculated. In summary, we present here VLP/P10 formulations as vaccine candidates against PCM, some of which have demonstrated for the first time their ability to prevent progression of this pernicious fungal disease, which represents a significant social burden in developing countries. [Mh] Termos MeSH primário: Antígenos de Fungos/imunologia Linfócitos T CD4-Positivos/imunologia Epitopos de Linfócito T/imunologia Proteínas Fúngicas/imunologia Vacinas Fúngicas/administração & dosagem Glicoproteínas/imunologia Paracoccidioides/crescimento & desenvolvimento Paracoccidioides/imunologia Paracoccidioidomicose/prevenção & controle [Mh] Termos MeSH secundário: Animais Citocinas/imunologia Citocinas/secreção Epitopos de Linfócito T/genética Vacinas Fúngicas/imunologia Vírus da Hepatite B/genética Imunização Epitopos Imunodominantes/imunologia Imunogenicidade da Vacina Memória Imunológica Fígado/microbiologia Pulmão/microbiologia Camundongos Endogâmicos BALB C Paracoccidioidomicose/imunologia Paracoccidioidomicose/microbiologia Baço/microbiologia Células Th1/imunologia Vacinas Sintéticas/administração & dosagem Vacinas Sintéticas/genética Vacinas Sintéticas/imunologia Vacinas de Partículas Semelhantes a Vírus/administração & dosagem Vacinas de Partículas Semelhantes a Vírus/genética Vacinas de Partículas Semelhantes a Vírus/imunologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (43 kDa protein, Paracoccidioides); 0 (Antigens, Fungal); 0 (Cytokines); 0 (Epitopes, T-Lymphocyte); 0 (Fungal Proteins); 0 (Fungal Vaccines); 0 (Glycoproteins); 0 (Immunodominant Epitopes); 0 (Vaccines, Synthetic); 0 (Vaccines, Virus-Like Particle) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171025 [Lr] Data última revisão: 171025 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170923 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pntd.0005927

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[PMID]: 28846733 [Au] Autor: Oliveira AF; Fernandes FF; Mariano VS; Almeida F; Ruas LP; Oliveira LL; Oliver C; Jamur MC; Roque-Barreira MC [Ad] Endereço: Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brasil. [Ti] Título: Paracoccin distribution supports its role in Paracoccidioides brasiliensis growth and dimorphic transformation. [So] Source: PLoS One;12(8):e0184010, 2017. [Is] ISSN: 1932-6203 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Paracoccidioides brasiliensis yeast was reported to express paracoccin, a GlcNAc-binding protein that displays N-acetyl-ß-d-glucosaminidase (NAGase) activity. Highly specific anti-paracoccin antibodies have been previously used to examine the localization of paracoccin in yeast and inhibit its growth in vitro. In the present study, anti-paracoccin antibodies were used to characterize, by scanning confocal microscopy, the distribution of paracoccin in P. brasiliensis hyphae, transition forms from hyphae to yeast, and mature yeast. In the mycelial phase, paracoccin was detected mainly in the hyphae tips, where it demonstrated a punctate distribution, and was associated with the cell wall. During the first 48 hours after a temperature shift from 26°C to 37°C, paracoccin expression in the differentiating hyphae was mainly detected in the budding regions, i.e. lateral protrusions, and inside the new daughter cells. There was an increased number of chlamydoconidia that expressed a high concentration of paracoccin on their surfaces and/or in their interiors 72-96 hours after the temperature shift. After 120 hours, yeast cells were the predominant form and their cytoplasm stained extensively for paracoccin, whereas Wheat Germ Agglutinin (WGA) staining was predominant on their exterior walls. After 10 days at 37°C, the interior of both mother and daughter yeast cells, as well as the budding regions, stained intensely for paracoccin. The comparison of mRNA-expression in the different fungal forms showed that PCN transcripts, although detected in all evaluated morphological forms, were higher in hypha and yeast-to-hypha transition forms. In conclusion, the pattern of paracoccin distribution in all P. brasiliensis morphotypes supports prevalent beliefs that it plays important roles in fungal growth and dimorphic transformation. [Mh] Termos MeSH primário: Proteínas Fúngicas/metabolismo Paracoccidioides/metabolismo [Mh] Termos MeSH secundário: Paracoccidioides/crescimento & desenvolvimento Aglutininas do Germe de Trigo/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Fungal Proteins); 0 (Wheat Germ Agglutinins) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171026 [Lr] Data última revisão: 171026 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170829 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pone.0184010

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[PMID]: 28500805 [Au] Autor: Lucinda LR; Polanski JF [Ad] Endereço: Department of Otorhinolaryngology, Hospital de Clínicas, Federal University of Paraná (UFPR), Curitiba, Brazil. [Ti] Título: Unusual Otolaryngologic Manifestations of Paracoccidioidomycosis: A Case Report and Review of Literature. [So] Source: Am J Trop Med Hyg;96(5):1136-1138, 2017 May. [Is] ISSN: 1476-1645 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: AbstractParacoccidioidomycosis is a systemic mycosis caused by . It occurs more frequently in its chronic form, which particularly affects male adults from rural areas. These patients present with pulmonary involvement and systemic symptoms. Skin and mucosal lesions are rather typical and might suggest the diagnosis. The involvement of the upper airway mucosa is common and the patients usually complain of dysphagia and dysphonia. Nonetheless, in endemic areas, physicians should maintain a high level of suspicion even when faced with some atypical symptoms. We present the case of an adult diagnosed with nasopharyngeal paracoccidioidomycosis after presenting with an unusual otolaryngologic syndrome including unilateral soft palate paralysis with velopharyngeal insufficiency and hearing loss secondary to middle ear effusion. [Mh] Termos MeSH primário: Perda Auditiva/diagnóstico Otite Média com Derrame/diagnóstico Paracoccidioides/isolamento & purificação Paracoccidioidomicose/diagnóstico [Mh] Termos MeSH secundário: Adulto Anti-Infecciosos Orelha Média/microbiologia Orelha Média/patologia Perda Auditiva/tratamento farmacológico Perda Auditiva/microbiologia Perda Auditiva/patologia Seres Humanos Masculino Ventilação da Orelha Média Nasofaringe/microbiologia Nasofaringe/patologia Otite Média com Derrame/tratamento farmacológico Otite Média com Derrame/microbiologia Otite Média com Derrame/patologia Palato Mole/microbiologia Palato Mole/patologia Paracoccidioides/efeitos dos fármacos Paracoccidioides/patogenicidade Paracoccidioidomicose/tratamento farmacológico Paracoccidioidomicose/microbiologia Paracoccidioidomicose/patologia Resultado do Tratamento Combinação Trimetoprima e Sulfametoxazol [Pt] Tipo de publicação: CASE REPORTS; JOURNAL ARTICLE [Nm] Nome de substância: 0 (Anti-Infective Agents); 8064-90-2 (Trimethoprim, Sulfamethoxazole Drug Combination) [Em] Mês de entrada: 1708 [Cu] Atualização por classe: 170803 [Lr] Data última revisão: 170803 [Sb] Subgrupo de revista: AIM; IM [Da] Data de entrada para processamento: 170514 [St] Status: MEDLINE [do] DOI: 10.4269/ajtmh.16-0937

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seleciona para imprimir Fotocópia Antunes, Edson Texto completo

[PMID]: 28489854 [Au] Autor: Braga FG; Ruas LP; Pereira RM; Lima XT; Antunes E; Mamoni RL; Blotta MHSL [Ad] Endereço: Department of Clinical Pathology, Faculty of Medical Sciences, State University of Campinas, Campinas, São Paulo, Brazil. [Ti] Título: Functional and phenotypic evaluation of eosinophils from patients with the acute form of paracoccidioidomycosis. [So] Source: PLoS Negl Trop Dis;11(5):e0005601, 2017 May. [Is] ISSN: 1935-2735 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: BACKGROUND: Eosinophilia is a typical finding of the acute/juvenile form of paracoccidioidomycosis (PCM), a systemic mycosis endemic in Latin America. This clinical form is characterized by depressed cellular immune response and production of Th2 cytokines. Moreover, it has been shown that the increased number of eosinophils in peripheral blood of patients returns to normal values after antifungal treatment. However, the role of eosinophils in PCM has never been evaluated. This study aimed to assess the phenotypic and functional characteristics of eosinophils in PCM. METHODS/PRINCIPAL FINDINGS: In 15 patients with the acute form of the disease, we detected expression of MBP, CCL5 (RANTES) and CCL11 (eotaxin) in biopsies of lymph nodes and liver. In addition, there were higher levels of chemokines and granule proteins in the peripheral blood of patients compared to controls. Isolation of eosinophils from blood revealed a higher frequency of CD69+ and TLR2+ eosinophils in patients compared to controls, and a lower population of CD80+ cells. We also evaluated the fungicidal capacity of eosinophils in vitro. Our results revealed that eosinophils from PCM patients and controls exhibit similar ability to kill P. brasiliensis yeast cells, although eosinophils of patients were less responsive to IL-5 stimulation than controls. CONCLUSION/PRINCIPAL FINDINGS: In conclusion, we suggest that eosinophils might play a role in the host response to fungi and in the pathophysiology of PCM by inducing an intense and systemic inflammatory response in the initial phase of the infection. [Mh] Termos MeSH primário: Eosinofilia/patologia Eosinófilos/imunologia Paracoccidioides/imunologia Paracoccidioidomicose/complicações Paracoccidioidomicose/patologia [Mh] Termos MeSH secundário: Adolescente Adulto Antígenos CD/análise Antígenos de Diferenciação de Linfócitos T/análise Antígeno B7-1/análise Criança Pré-Escolar Citocinas/sangue Eosinófilos/química Feminino Seres Humanos Lectinas Tipo C/análise Masculino [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antigens, CD); 0 (Antigens, Differentiation, T-Lymphocyte); 0 (B7-1 Antigen); 0 (CD69 antigen); 0 (Cytokines); 0 (Lectins, C-Type) [Em] Mês de entrada: 1707 [Cu] Atualização por classe: 171116 [Lr] Data última revisão: 171116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170511 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pntd.0005601

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seleciona para imprimir Fotocópia Texto completo SciELO Brasil

[PMID]: 28380215 [Au] Autor: Peron G; Fernandes FF; Landgraf TN; Martinez R; Panunto-Castelo A [Ad] Endereço: Departamento de Biologia Estrutural e Funcional, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, Brasil. [Ti] Título: Recombinant 60-kDa heat shock protein from Paracoccidioides brasiliensis: is it a good antigen for serological diagnosis of paracoccidioidomycosis? [So] Source: Braz J Med Biol Res;50(4):e5928, 2017 Apr 03. [Is] ISSN: 1414-431X [Cp] País de publicação: Brazil [La] Idioma: eng [Ab] Resumo: Paracoccidioides brasiliensis and P. lutzii are fungi that cause paracoccidioidomycosis (PCM), the most prevalent systemic mycosis in South America. For serological diagnosis, although 43-kDa glycoprotein (gp43) is regarded as highly specific for PCM, the occurrence of false negative reactions in sera from patients infected with P. lutzii suggests that preparation with only one antigen is not recommended. Heat shock proteins are feasible alternatives as a second antigen because they are often highly immunogenic. In this study, we evaluated the usefulness of recombinant 60-kDa heat shock protein from P. brasiliensis (rPbHsp60) for the serological diagnosis of PCM. Using western blotting assay, we observed that 77.3% of the sera from PCM patients were positive to rPbHsp60, with 90.9% positivity to recombinant gp43 (rgp43). More importantly, sera from healthy subjects had 27% positivity to rPbHsp60 and none to rgp43. When rPbHsp60 was used in ELISA, we did not observe significant differences between the reactions with sera from PCM patients and healthy subjects, while the difference was clearly evident when the antigen was rgp43. Furthermore, rPbHsp60 was recognized by sera from patients with histoplasmosis, aspergillosis, sporotrichosis or tuberculosis in an ELISA test. These results show that rPbHsp60 is not a good antigen for PCM diagnosis. [Mh] Termos MeSH primário: Antígenos de Fungos/sangue Chaperonina 60/sangue Proteínas Fúngicas/sangue Paracoccidioides/imunologia Paracoccidioidomicose/diagnóstico Testes Sorológicos/métodos [Mh] Termos MeSH secundário: Western Blotting Eletroforese em Gel de Poliacrilamida Ensaio de Imunoadsorção Enzimática Seres Humanos Paracoccidioidomicose/sangue Proteínas Recombinantes/sangue Valores de Referência Reprodutibilidade dos Testes Estatísticas não Paramétricas [Pt] Tipo de publicação: EVALUATION STUDIES; JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antigens, Fungal); 0 (Chaperonin 60); 0 (Fungal Proteins); 0 (Recombinant Proteins) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 170906 [Lr] Data última revisão: 170906 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170406 [St] Status: MEDLINE

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[PMID]: 28355221 [Au] Autor: de Macedo PM; Almeida-Paes R; Freitas DF; Varon AG; Paixão AG; Romão AR; Coutinho ZF; Pizzini CV; Zancopé-Oliveira RM; Francesconi do Valle AC [Ad] Endereço: Infectious Dermatology Clinical Research Laboratory, Evandro Chagas National Institute of Infectious Diseases, Fiocruz, Rio de Janeiro, Brazil. [Ti] Título: Acute juvenile Paracoccidioidomycosis: A 9-year cohort study in the endemic area of Rio de Janeiro, Brazil. [So] Source: PLoS Negl Trop Dis;11(3):e0005500, 2017 Mar. [Is] ISSN: 1935-2735 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: BACKGROUND: Paracoccidioidomycosis (PCM) is a systemic mycosis caused by pathogenic dimorphic fungi of the genus Paracoccidioides. It is the most important systemic mycosis in Latin America and the leading cause of hospitalizations and death among them in Brazil. Acute PCM is less frequent but relevant because vulnerable young patients are affected and the severity is usually higher than that of the chronic type. METHODS: The authors performed a retrospective cohort study from 2001 to 2009 including acute juvenile PCM patients from a reference center in Rio de Janeiro, Brazil. Clinical, epidemiological, diagnostic, therapeutic, and prognostic data were reported. RESULTS: Twenty-nine patients were included. The average age was 23 years old and the male to female ratio was 1:1.07. All cases were referred from 3 of 9 existing health areas in the state of Rio de Janeiro, predominantly from urban areas (96.5%). Lymph nodes were the most affected organs (100%), followed by the skin and the spleen (31% each). Twenty-eight patients completed treatment (median 25 months) and progressed to clinical and serological cure; 1 death occurred. Twenty-four patients completed 48-month median follow-up. Four patients abandoned follow-up after the end of treatment. The most frequent sequela was low adrenal reserve. Paracoccidioides brasiliensis S1 was identified by partial sequencing of the arf and gp43 genes from 4 patients who presented a viable fungal culture. CONCLUSION: Acute juvenile PCM is a severe disease with a high rate of complications. There are few cohort clinical studies of acute PCM in the literature. More studies should be developed to promote improvement in patients' healthcare. [Mh] Termos MeSH primário: Doenças Endêmicas Paracoccidioides/isolamento & purificação Paracoccidioidomicose/epidemiologia [Mh] Termos MeSH secundário: Adolescente Adulto Antifúngicos/uso terapêutico Brasil/epidemiologia Criança Estudos de Coortes Feminino Proteínas Fúngicas/genética Genótipo Seres Humanos Masculino Paracoccidioides/classificação Paracoccidioides/genética Paracoccidioidomicose/diagnóstico Paracoccidioidomicose/tratamento farmacológico Paracoccidioidomicose/patologia Estudos Retrospectivos Análise de Sequência de DNA Resultado do Tratamento Adulto Jovem [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antifungal Agents); 0 (Fungal Proteins) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 170619 [Lr] Data última revisão: 170619 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170330 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pntd.0005500

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seleciona para imprimir Fotocópia Burger, Eva Texto completo

[PMID]: 28349345 [Au] Autor: Dos Santos LFM; Melo NB; de Carli ML; Mendes ACSC; Bani GMAC; Verinaud LM; Burger E; de Oliveira I Moraes G; Pereira AAC; Brigagão MRL; Hanemann JAC; Sperandio FF [Ad] Endereço: School of Dentistry, Federal University of Alfenas (UNIFAL-MG), Alfenas, MG, 37130-000, Brazil. [Ti] Título: Photodynamic inactivation of Paracoccidioides brasiliensis helps the outcome of oral paracoccidiodomycosis. [So] Source: Lasers Med Sci;32(4):921-930, 2017 May. [Is] ISSN: 1435-604X [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: The antifungal drug therapy often employed to treat paracoccidiodomycosis (PCM), an important neglected fungal systemic infection, leads to offensive adverse effects, besides being very long-lasting. In addition, PCM compromises the oral health of patients by leading to oral lesions that are very painful and disabling. In that way, photodynamic therapy (PDT) arises as a new promising adjuvant treatment for inactivating Paracoccidioides brasiliensis (Pb), the responsible fungus for PCM, and also for helping the patients to deal with such debilitating oral lesions. PDT has been linked to an improved microbial killing, also presenting the advantage of not inducing immediate microbial resistance such as drugs. For the present study, we investigated the generation of reactive oxygen species (ROS) by using the fluorescent probes hydroxyphenyl fluorescein (HPF) and aminophenyl fluorescein (APF) after toluidine blue (TBO-37.5 mg/L)-mediated PDT (660 nm, 40 mW, and 0.04 cm spot area) and the action of TBO-PDT upon Pb cultures grown for 7 or 15 days in semisolid Fava Netto's culture medium; we also targeted oral PCM manifestations by reporting the first clinical cases (three patients) to receive topic PDT for such purpose. We were able to show a significant generation of hydroxyl radicals and hypochlorite after TBO-PDT with doses around 90 J/cm ; such ROS generation was particularly useful to attack and inactivate Pb colonies at 7 and 15 days. All three patients reported herein related an immediate relief when it came to pain, mouth opening, and also the ability to chew and swallow. As extracted from our clinical results, which are in fact based on in vitro outcomes, TBO-PDT is a very safe, inexpensive, and promising therapy for the oral manifestations of PCM. [Mh] Termos MeSH primário: Viabilidade Microbiana/efeitos dos fármacos Doenças da Boca/tratamento farmacológico Doenças da Boca/microbiologia Paracoccidioides/efeitos da radiação Paracoccidioidomicose/tratamento farmacológico Paracoccidioidomicose/microbiologia Fotoquimioterapia Cloreto de Tolônio/uso terapêutico [Mh] Termos MeSH secundário: Adulto Antifúngicos/farmacologia Corantes Fluorescentes/química Seres Humanos Cinética Masculino Meia-Idade Doenças da Boca/patologia Paracoccidioides/crescimento & desenvolvimento Espécies Reativas de Oxigênio/metabolismo Cloreto de Tolônio/farmacologia [Pt] Tipo de publicação: CASE REPORTS; CLINICAL TRIAL; JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antifungal Agents); 0 (Fluorescent Dyes); 0 (Reactive Oxygen Species); 15XUH0X66N (Tolonium Chloride) [Em] Mês de entrada: 1707 [Cu] Atualização por classe: 171013 [Lr] Data última revisão: 171013 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170329 [St] Status: MEDLINE [do] DOI: 10.1007/s10103-017-2193-y

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[PMID]: 28295653 [Au] Autor: Mendes JF; Klafke GB; Albano APN; Cabana ÂL; Teles AJ; de Camargo ZP; Xavier MO; Meireles MCA [Ad] Endereço: Center of Diagnosis in Veterinary Mycology, Department of Veterinary Preventive, Faculty of Veterinary, University Federal of Pelotas (UFPel), Pelotas, RS, Brazil. [Ti] Título: Paracoccidioidomycosis infection in domestic and wild mammals by Paracoccidioides lutzii. [So] Source: Mycoses;60(6):402-406, 2017 Jun. [Is] ISSN: 1439-0507 [Cp] País de publicação: Germany [La] Idioma: eng [Ab] Resumo: Paracoccidioidomycosis (PCM) is a systemic mycosis that occurs in several Latin American countries, especially in Brazil. It is caused by the thermo-dimorphic fungus Paracoccidioides spp. Serological studies to detect animal infection represent an excellent strategy for data on the agent's ecology. Although the state of Rio Grande do Sul (RS) is an endemic area for PCM in humans, there is scarce information available on the ecology of the agent in the region. This study aimed to investigate the infection by Paracoccidioides lutzii in animals living in RS, Brazil. A total of 85 wild mammals, 200 horses and 196 domestic dogs, previously tested for infection by P. brasiliensis, were included in this study. Serum samples from the animals were tested by ELISA to detect anti- P. lutzii antibodies. From the 481 animals tested, 105 (21.8%) were seropositive for IgG anti-P. lutzii. Of these, 54 were also positive for P. brasiliensis. A total of 11 horses (10.5%), 30 dogs (28.8%) and 10 wild mammals (9.5%) were positive only for P. lutzii (n=51). The detection of anti-P. lutzii antibodies in animals of RS suggests that the fungus can be found in southern Brazil, despite being described mainly in the midwest and southeast of the country. [Mh] Termos MeSH primário: Mamíferos/microbiologia Paracoccidioides/isolamento & purificação Paracoccidioidomicose/veterinária [Mh] Termos MeSH secundário: Animais Anticorpos Antifúngicos/sangue Antígenos de Fungos/imunologia Brasil/epidemiologia Cães/microbiologia Ensaio de Imunoadsorção Enzimática Cavalos/microbiologia Seres Humanos Paracoccidioidomicose/epidemiologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antibodies, Fungal); 0 (Antigens, Fungal) [Em] Mês de entrada: 1708 [Cu] Atualização por classe: 170810 [Lr] Data última revisão: 170810 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170316 [St] Status: MEDLINE [do] DOI: 10.1111/myc.12608

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[PMID]: 28279786 [Au] Autor: Rossi DC; Spadari CC; Nosanchuk JD; Taborda CP; Ishida K [Ad] Endereço: Laboratório de Fungos Dimórficos Patogênicos, Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, Brazil. [Ti] Título: Miltefosine is fungicidal to Paracoccidioides spp. yeast cells but subinhibitory concentrations induce melanisation. [So] Source: Int J Antimicrob Agents;49(4):465-471, 2017 Apr. [Is] ISSN: 1872-7913 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: Paracoccidioidomycosis (PCM) is a systemic mycosis caused by the dimorphic fungi Paracoccidioides spp. The duration of antifungal treatment ranges from months to years and relapses may nevertheless occur despite protracted therapy. Thus, there remains an urgent need for new therapeutic options. Miltefosine (MLT), an analogue of alkylphospholipids, has antifungal activity against species of yeast and filamentous fungi. The aim of this study was to evaluate the antifungal effects of MLT on the yeast forms of Paracoccidioides brasiliensis and Paracoccidioides lutzii. MLT demonstrated inhibitory activity from 0.12 to 1 µg/mL, which was similar to amphotericin B or the combination trimethoprim/sulfamethoxazole but was not more effective than itraconazole. The fungicidal activity of MLT occurred at concentrations ≥1 µg/mL. Ultrastructural alterations were observed following exposure of the fungus to a subinhibitory concentration of MLT, such as cytoplasmic membrane alteration, mitochondrial swelling, electron-lucent vacuole accumulation and increasing melanosome-like structures. Melanin production by yeasts following MLT exposure was confirmed by labelling with antibodies to melanin. In addition, the combination of a subinhibitory concentration of MLT and tricyclazole, an inhibitor of DHN-melanin biosynthesis, drastically reduced yeast viability. In conclusion, MLT had a fungicidal effect against both Paracoccidioides spp., and a subinhibitory concentration impacted melanogenesis. These findings suggest that additional investigations should be pursued to establish a role for MLT in the treatment of PCM. [Mh] Termos MeSH primário: Antifúngicos/farmacologia Melaninas/biossíntese Paracoccidioides/efeitos dos fármacos Paracoccidioides/metabolismo Fosforilcolina/análogos & derivados [Mh] Termos MeSH secundário: Animais Membrana Celular/efeitos dos fármacos Membrana Celular/ultraestrutura Cães Sinergismo Farmacológico Seres Humanos Testes de Sensibilidade Microbiana Viabilidade Microbiana/efeitos dos fármacos Organelas/efeitos dos fármacos Organelas/ultraestrutura Paracoccidioides/isolamento & purificação Paracoccidioides/ultraestrutura Fosforilcolina/farmacologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antifungal Agents); 0 (Melanins); 107-73-3 (Phosphorylcholine); 53EY29W7EC (miltefosine) [Em] Mês de entrada: 1704 [Cu] Atualização por classe: 170417 [Lr] Data última revisão: 170417 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170311 [St] Status: MEDLINE

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