|
[PMID]: | 28489938 |
[Au] Autor: | Hu W; Pan X; Abbas HMK; Li F; Dong W |
[Ad] Endereço: | Department of Plant Pathology, College of Plant Science and Technology and the Key Lab of Crop Disease Monitoring & Safety Control in Hubei Province, Huazhong Agricultural University, Wuhan, Hubei Province, China. |
[Ti] Título: | Metabolites contributing to Rhizoctonia solani AG-1-IA maturation and sclerotial differentiation revealed by UPLC-QTOF-MS metabolomics. |
[So] Source: | PLoS One;12(5):e0177464, 2017. | [Is] ISSN: | 1932-6203 |
[Cp] País de publicação: | United States |
[La] Idioma: | eng |
[Ab] Resumo: | Rhizoctonia solani is a causative agent of sheath blight, which results in huge economic losses every year. During its life cycle, the formation of sclerotia helps Rhizoctonia solani withstand a variety of unfavorable factors. Oxidative stress is a key factor that induces sclerotium formation. The differentiated and undifferentiated phenotypes of R. solani AG-1-IA were obtained by controlling aerial conditions. Metabolomics based on the mass spectrometry technique combined with multivariate and univariate analyses was used to investigate the metabolic variation in vegetative, differentiated and undifferentiated mycelia. Our results revealed that during maturation, the metabolic levels of N2-acetyl-L-ornithine, 3,1'-(OH)2-Gamma-carotene, (5Z,7E)-(1S,3R)-24,24-difluoro-24a-homo-9,10-seco-5,7,10(19)-cholestatrien-1,3,25-triol, stoloniferone O, PA(O-18:0/12:0), PA(P-16:0/14:0), PA(P-16:0/16:(19Z)) and PA(P-16:0/17:2(9Z,12Z)) were suppressed in both differentiated and undifferentiated mycelia. The concentrations of PE(20:1(11Z)/14:1(9Z)), PE(P-16:0/20:4(5Z,8Z,11Z,13E)(15OH[S])) and PS(12:0/18:1(9Z)) were increased in the differentiated group, while increased levels of N(gamma)-nitro-L-arginine, tenuazonic acid and 9S,10S,11R-trihydroxy-12Z,15Z-octadecadienoic acid were found in the undifferentiated group. Our results suggest that different levels of these metabolites may act as biomarkers for the developmental stages of R. solani AG-1-IA. Moreover, the mechanisms of sclerotium formation and mycelium differentiation were elucidated at the metabolic level. |
[Mh] Termos MeSH primário: |
Micélio/crescimento & desenvolvimento Micélio/metabolismo Rhizoctonia/crescimento & desenvolvimento Rhizoctonia/metabolismo
|
[Mh] Termos MeSH secundário: |
Cromatografia Líquida de Alta Pressão Espectrometria de Massas Metabolômica Micélio/química Fenótipo Doenças das Plantas/microbiologia Rhizoctonia/química
|
[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Em] Mês de entrada: | 1709 |
[Cu] Atualização por classe: | 170908 |
[Lr] Data última revisão:
| 170908 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 170511 |
[St] Status: | MEDLINE |
[do] DOI: | 10.1371/journal.pone.0177464 |
|
|
|