Base de dados : MEDLINE
Pesquisa : B01.300.665.600 [Categoria DeCS]
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[PMID]:27998268
[Au] Autor:Seppälä S; Solomon KV; Gilmore SP; Henske JK; O'Malley MA
[Ad] Endereço:Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet Bygning 220, 2800, Kgs. Lyngby, Denmark.
[Ti] Título:Mapping the membrane proteome of anaerobic gut fungi identifies a wealth of carbohydrate binding proteins and transporters.
[So] Source:Microb Cell Fact;15(1):212, 2016 Dec 20.
[Is] ISSN:1475-2859
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Engineered cell factories that convert biomass into value-added compounds are emerging as a timely alternative to petroleum-based industries. Although often overlooked, integral membrane proteins such as solute transporters are pivotal for engineering efficient microbial chassis. Anaerobic gut fungi, adapted to degrade raw plant biomass in the intestines of herbivores, are a potential source of valuable transporters for biotechnology, yet very little is known about the membrane constituents of these non-conventional organisms. Here, we mined the transcriptome of three recently isolated strains of anaerobic fungi to identify membrane proteins responsible for sensing and transporting biomass hydrolysates within a competitive and rather extreme environment. RESULTS: Using sequence analyses and homology, we identified membrane protein-coding sequences from assembled transcriptomes from three strains of anaerobic gut fungi: Neocallimastix californiae, Anaeromyces robustus, and Piromyces finnis. We identified nearly 2000 transporter components: about half of these are involved in the general secretory pathway and intracellular sorting of proteins; the rest are predicted to be small-solute transporters. Unexpectedly, we found a number of putative sugar binding proteins that are associated with prokaryotic uptake systems; and approximately 100 class C G-protein coupled receptors (GPCRs) with non-canonical putative sugar binding domains. CONCLUSIONS: We report the first comprehensive characterization of the membrane protein machinery of biotechnologically relevant anaerobic gut fungi. Apart from identifying conserved machinery for protein sorting and secretion, we identify a large number of putative solute transporters that are of interest for biotechnological applications. Notably, our data suggests that the fungi display a plethora of carbohydrate binding domains at their surface, perhaps as a means to sense and sequester some of the sugars that their biomass degrading, extracellular enzymes produce.
[Mh] Termos MeSH primário: Carboidratos
Proteínas Fúngicas/metabolismo
Fungos/metabolismo
Intestinos/microbiologia
Proteínas de Membrana/metabolismo
Proteoma/metabolismo
[Mh] Termos MeSH secundário: Anaerobiose
Animais
Fezes/microbiologia
Proteínas Fúngicas/genética
Fungos/classificação
Fungos/genética
Perfilação da Expressão Gênica/métodos
Cabras
Cavalos
Lignina/metabolismo
Proteínas de Membrana/genética
Proteínas de Membrana Transportadoras/genética
Proteínas de Membrana Transportadoras/metabolismo
Neocallimastigales/genética
Neocallimastigales/metabolismo
Piromyces/genética
Piromyces/metabolismo
Ligação Proteica
Proteoma/genética
Ovinos
Especificidade da Espécie
Transcriptoma/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carbohydrates); 0 (Fungal Proteins); 0 (Membrane Proteins); 0 (Membrane Transport Proteins); 0 (Proteome); 11132-73-3 (lignocellulose); 9005-53-2 (Lignin)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170410
[Lr] Data última revisão:
170410
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161222
[St] Status:MEDLINE
[do] DOI:10.1186/s12934-016-0611-7


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[PMID]:27026397
[Au] Autor:Gruninger RJ; Cote C; McAllister TA; Abbott DW
[Ad] Endereço:Lethbridge Research Centre, Agriculture and Agri-Food Canada, 5403-1st Ave South, Lethbridge, AB, Canada, T1J 4B1 robert.gruninger@agr.gc.ca wade.abbott@agr.gc.ca.
[Ti] Título:Contributions of a unique ß-clamp to substrate recognition illuminates the molecular basis of exolysis in ferulic acid esterases.
[So] Source:Biochem J;473(7):839-49, 2016 Apr 01.
[Is] ISSN:1470-8728
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Lignocellulosic biomass is a promising renewable resource; however, deconstruction of this material is still the rate-limiting step. Major obstacles in the biocatalytic turnover of lignocellulose are ester-linked decorations that prevent access to primary structural polysaccharides. Enzymes targeting these esters represent promising biotools for increasing bioconversion efficiency. Ruminant livestock are unique in their ability to degrade lignocellulose through the action of their gut microbiome. The anaerobic fungi (phylum Neocallimastigomycota) are key members of this ecosystem that express a large repertoire of carbohydrate-active enzymes (CAZymes) with little sequence identity with characterized CAZymes [Lombard, Golaconda, Drula, Coutinho and Henrissat (2014) Nucleic Acids Res. 42: , D490-D495]. We have identified a carbohydrate esterase family 1 (CE1) ferulic acid esterase (FAE) belonging to Anaeromyces mucronatus(AmCE1/Fae1a), and determined its X-ray structure in both the presence [1.55 Å (1 Å=0.1 nm)] and absence (1.60 Å) of ferulic acid. AmCE1 adopts an α/ß-hydrolase fold that is structurally conserved with bacterial FAEs, and possesses a unique loop, termed the ß-clamp, that encloses the ligand. Isothermal titration calorimetry reveals that substrate binding is driven by enthalpic contributions, which overcomes a large entropic penalty. A comparative analysis of AmCE1 with related enzymes has uncovered the apparent structural basis for differential FAE activities targeting cross-linking ferulic acid conjugates compared with terminal decorations. Based on comparisons to structurally characterized FAEs, we propose that the ß-clamp may define the structural basis of exolytic activities in FAEs. This provides a structure-based tool for predicting exolysis and endolysis in CE1. These insights hold promise for rationally identifying enzymes tailored for bioconversion of biomass with variations in cell wall composition.
[Mh] Termos MeSH primário: Hidrolases de Éster Carboxílico/química
Ácidos Cumáricos/química
Proteínas Fúngicas/química
Neocallimastigales/enzimologia
[Mh] Termos MeSH secundário: Hidrolases de Éster Carboxílico/metabolismo
Ácidos Cumáricos/metabolismo
Proteínas Fúngicas/metabolismo
Estrutura Secundária de Proteína
Estrutura Terciária de Proteína
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Coumaric Acids); 0 (Fungal Proteins); AVM951ZWST (ferulic acid); EC 3.1.1.- (Carboxylic Ester Hydrolases); EC 3.1.1.73 (feruloyl esterase)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:160330
[Lr] Data última revisão:
160330
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160331
[St] Status:MEDLINE
[do] DOI:10.1042/BJ20151153


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[PMID]:26645659
[Au] Autor:Li YC; Li GY; Gou M; Xia ZY; Tang YQ; Kida K
[Ad] Endereço:College of Light Industry, Textile and Food Engineering, Sichuan University, No. 24, South Section 1, First Ring Road, Chengdu 610065, China; College of Architecture and Environment, Sichuan University, No. 24, South Section 1, First Ring Road, Chengdu 610065, China.
[Ti] Título:Functional expression of xylose isomerase in flocculating industrial Saccharomyces cerevisiae strain for bioethanol production.
[So] Source:J Biosci Bioeng;121(6):685-691, 2016 Jun.
[Is] ISSN:1347-4421
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Saccharomyces cerevisiae strains with xylose isomerase (XI) pathway were constructed using a flocculating industrial strain (YC-8) as the host. Both strains expressing wild-type xylA (coding XI) from the fungus Orpinomyces sp. and the bacterium Prevotella ruminicola, respectively, showed better growth ability and fermentation capacity when using xylose as the sole sugar than most of the reported strains expressing XI. Codon optimization of both XIs did not improve the xylose fermentation ability of the strains. Adaption significantly increased XI activity resulting in improved growth and fermentation. The strains expressing codon-optimized XI showed a higher increase in xylose consumption and ethanol production compared to strains expressing wild XI. Among all strains, the adapted strain YCPA2E expressing XI from P. ruminicola showed the best performance in the fermentation of xylose to ethanol. After 48 h of fermentation, YCPA2E assimilated 16.95 g/L xylose and produced 6.98 g/L ethanol. These results indicate that YC-8 is a suitable host strain for XI expression, especially for the codon-optimized XI originating from P. ruminicola.
[Mh] Termos MeSH primário: Aldose-Cetose Isomerases/genética
Aldose-Cetose Isomerases/metabolismo
Reatores Biológicos
Etanol/metabolismo
Fermentação
Saccharomyces cerevisiae/genética
Saccharomyces cerevisiae/metabolismo
[Mh] Termos MeSH secundário: Aldose-Cetose Isomerases/biossíntese
Códon/genética
Etanol/provisão & distribuição
Floculação
Neocallimastigales/enzimologia
Neocallimastigales/genética
Prevotella ruminicola/enzimologia
Prevotella ruminicola/genética
Xilose/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Codon); 3K9958V90M (Ethanol); A1TA934AKO (Xylose); EC 5.3.1.- (Aldose-Ketose Isomerases); EC 5.3.1.5 (xylose isomerase)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170830
[Lr] Data última revisão:
170830
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151210
[St] Status:MEDLINE


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[PMID]:26228561
[Au] Autor:Dagar SS; Kumar S; Griffith GW; Edwards JE; Callaghan TM; Singh R; Nagpal AK; Puniya AK
[Ad] Endereço:Dairy Microbiology Division, ICAR-National Dairy Research Institute, Karnal 132001, India. Electronic address: dagarsumit@gmail.com.
[Ti] Título:A new anaerobic fungus (Oontomyces anksri gen. nov., sp. nov.) from the digestive tract of the Indian camel (Camelus dromedarius).
[So] Source:Fungal Biol;119(8):731-7, 2015 Aug.
[Is] ISSN:1878-6146
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Two cultures of anaerobic fungi were isolated from the forestomach of an Indian camel (Camelus dromedarius). Phylogenetic analysis using both the internal transcribed spacer (ITS) and large-subunit (LSU) regions of the rRNA locus demonstrated that these isolates were identical and formed a distinct clade within the anaerobic fungi (phylum Neocallimastigomycota). Morphological examination showed that these fungi formed monocentric thalli with filamentous rhizoids and uniflagellate zoospores, broadly similar to members of the genus Piromyces. However, distinctive morphological features were observed, notably the pinching of the cytoplasm in the sporangiophore and the formation of intercalary rhizoidal swellings. Since genetic analyses demonstrated this fungus was only distantly related to Piromyces spp. and closer to the polycentric Anaeromyces clade, we have assigned it to a new genus and species Oontomyces anksri gen. nov., sp. nov. Interrogation of the GenBank database identified several closely related ITS sequences, which were all environmental sequences obtained from camels, raising the possibility that this fungus may be specific to camelids.
[Mh] Termos MeSH primário: Camelus/microbiologia
Trato Gastrointestinal/microbiologia
Neocallimastigales/classificação
Neocallimastigales/isolamento & purificação
[Mh] Termos MeSH secundário: Anaerobiose
Animais
Análise por Conglomerados
DNA Fúngico/química
DNA Fúngico/genética
DNA Ribossômico/química
DNA Ribossômico/genética
DNA Espaçador Ribossômico/química
DNA Espaçador Ribossômico/genética
Microscopia
Dados de Sequência Molecular
Neocallimastigales/citologia
Neocallimastigales/genética
Filogenia
RNA Ribossômico/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Fungal); 0 (DNA, Ribosomal); 0 (DNA, Ribosomal Spacer); 0 (RNA, Ribosomal); 0 (RNA, ribosomal, 26S)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150801
[St] Status:MEDLINE


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[PMID]:25889970
[Au] Autor:Li P; Sun H; Chen Z; Li Y; Zhu T
[Ad] Endereço:CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China. real_lpf@163.com.
[Ti] Título:Construction of efficient xylose utilizing Pichia pastoris for industrial enzyme production.
[So] Source:Microb Cell Fact;14:22, 2015 Feb 21.
[Is] ISSN:1475-2859
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Cellulosic biomass especially agricultural/wood residues can be utilized as feedstock to cost-effectively produce fuels, chemicals and bulk industrial enzymes, which demands xylose utilization from microbial cell factories. While previous works have made significant progress in improving microbial conversion of xylose into fuels and chemicals, no study has reported the engineering of efficient xylose utilizing protein expression systems for the purpose of producing industrial enzymes. RESULTS: In this work, using Pichia pastoris as an example, we demonstrated the successful engineering of xylose metabolizing ability into of protein expression systems. A heterologous XI (xylose isomerase) pathway was introduced into P. pastoris GS115 by overexpressing the Orpinomyces spp. XI or/and the endogenous XK (xylulokinase) gene, and evolutionary engineering strategies were also applied. Results showed that the XI pathway could be functionally expressed in P. pastoris. After 50 generation of sequential batch cultivation, a set of domesticated recombinant P. pastoris strains with different performance metrics on xylose were obtained. One evolved strain showed the highest xylose assimilation ability, whose cell yield on xylose can even be comparable to that on glucose or glycerol. This strain also showed significantly increased ß-mannanase production when cultured on xylose medium. Furthermore, transcription analysis of xylose pathway genes suggested that overexpression of XI and XK might be the key factors affecting effective xylose assimilation. CONCLUSIONS: To our best knowledge, this study is the first work demonstrating the construction of efficient xylose utilizing P. pastoris strains, thus providing a basis for using cellulosic biomass for bulk industrial enzyme production.
[Mh] Termos MeSH primário: Proteínas Fúngicas/metabolismo
Fosfotransferases (Aceptor do Grupo Álcool)/metabolismo
Pichia/metabolismo
[Mh] Termos MeSH secundário: Aldose-Cetose Isomerases/genética
Aldose-Cetose Isomerases/metabolismo
Biomassa
Proteínas Fúngicas/genética
Neocallimastigales/enzimologia
Fosfotransferases (Aceptor do Grupo Álcool)/genética
Plasmídeos/genética
Plasmídeos/metabolismo
Proteínas Recombinantes/biossíntese
Proteínas Recombinantes/química
Proteínas Recombinantes/isolamento & purificação
Xilose/metabolismo
beta-Manosidase/genética
beta-Manosidase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Fungal Proteins); 0 (Recombinant Proteins); A1TA934AKO (Xylose); EC 2.7.1.- (Phosphotransferases (Alcohol Group Acceptor)); EC 2.7.1.17 (xylulokinase); EC 3.2.1.25 (beta-Mannosidase); EC 5.3.1.- (Aldose-Ketose Isomerases); EC 5.3.1.5 (xylose isomerase)
[Em] Mês de entrada:1601
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150419
[St] Status:MEDLINE
[do] DOI:10.1186/s12934-015-0206-8


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[PMID]:25495284
[Au] Autor:Lee SM; Guan LL; Eun JS; Kim CH; Lee SJ; Kim ET; Lee SS
[Ad] Endereço:Division of Applied Life Sciences (BK21+), Institute of Agriculture & Life Sciences, Graduate School of Gyeongsang National University, Jinju, Korea.
[Ti] Título:The effect of anaerobic fungal inoculation on the fermentation characteristics of rice straw silages.
[So] Source:J Appl Microbiol;118(3):565-73, 2015 Mar.
[Is] ISSN:1365-2672
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:AIMS: To identify whether the supplement of anaerobic fungi isolates with cellulolytic activities accelerates the silage fermentation. METHODS AND RESULTS: Three fungal isolates with the highest cellulolytic activities among 45 strains of anaerobic fungal stock in our laboratory were selected and used as silage inoculants. The rice straw (RS) was ensiled for 10, 30, 60, 90 and 120 days with four treatments of anaerobic fungi derived from the control (no fungus), Piromyces M014 (isolated from the rumen of the Korean native goat), Orpinomyces R001 (isolated from the duodenum of Korean native cattle) and Neocallimastix M010 (isolated from the guts of termites), respectively. The silages inoculated with pure strains of fungi showed a higher fungal population (P < 0.05) when compared to the control silage. In situ ruminal DM disappearance of RS silage (RSS) was improved with fungal treatment. SEM observation showed live fungal cells inoculated in RS could survive during the ensiling process. Overall, this study indicated that the inoculation of anaerobic fungi decreased the cell wall content of the RSS and increased in situ dry matter disappearance. CONCLUSIONS: The supplementation of anaerobic fungi isolates to RSS as a silage inoculant improves the RSS quality. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first study showing the potential application of supplement of anaerobic fungi isolated from the guts may be applied industrially as an alternate feed additive that improves the silage quality.
[Mh] Termos MeSH primário: Fermentação
Fungos/metabolismo
Oryza
Silagem
[Mh] Termos MeSH secundário: Anaerobiose
Animais
Bovinos
Neocallimastigales/isolamento & purificação
Neocallimastix/isolamento & purificação
Piromyces/isolamento & purificação
Rúmen/microbiologia
Silagem/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1510
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141216
[St] Status:MEDLINE
[do] DOI:10.1111/jam.12724


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[PMID]:25367149
[Au] Autor:Struchtemeyer CG; Ranganathan A; Couger MB; Liggenstoffer AS; Youssef NH; Elshahed MS
[Ad] Endereço:1] Department of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater, Oklahoma 74074 [2] Department of Biology and Health Sciences, McNeese State University, Lake Charles, Louisiana 70609.
[Ti] Título:Survival of the anaerobic fungus Orpinomyces sp. strain C1A after prolonged air exposure.
[So] Source:Sci Rep;4:6892, 2014 Nov 04.
[Is] ISSN:2045-2322
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Anaerobic fungi are efficient plant biomass degraders and represent promising agents for a variety of biotechnological applications. We evaluated the tolerance of an anaerobic fungal isolate, Orpinomyces sp. strain C1A, to air exposure in liquid media using soluble (cellobiose) and insoluble (dried switchgrass) substrates. Strain C1A grown on cellobiose survived for 11, and 13.5 hours following air exposure when grown under planktonic, and immobilized conditions, respectively. When grown on switchgrass media, strain C1A exhibited significantly enhanced air tolerance and survived for 168 hours. The genome of strain C1A lacked a catalase gene, but contained superoxide dismutase and glutathione peroxidase genes. Real time PCR analysis indicated that superoxide dismutase, but not glutathione peroxidase, exhibits a transient increase in expression level post aeration. Interestingly, the C1A superoxide dismutase gene of strain C1A appears to be most closely related to bacterial SODs, which implies its acquisition from a bacterial donor via cross kingdom horizontal gene transfer during Neocallimastigomycota evolution. We conclude that strain C1A utilizes multiple mechanisms to minimize the deleterious effects of air exposure such as physical protection and the production of oxidative stress enzymes.
[Mh] Termos MeSH primário: Neocallimastigales/fisiologia
[Mh] Termos MeSH secundário: Ar
Anaerobiose
Celobiose/metabolismo
Meios de Cultura
Proteínas Fúngicas/biossíntese
Proteínas Fúngicas/genética
Expressão Gênica
Regulação Enzimológica da Expressão Gênica
Regulação Fúngica da Expressão Gênica
Genes Fúngicos
Viabilidade Microbiana
Oxigênio/fisiologia
Filogenia
Estresse Fisiológico
Superóxido Dismutase/biossíntese
Superóxido Dismutase/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Culture Media); 0 (Fungal Proteins); 16462-44-5 (Cellobiose); EC 1.15.1.1 (Superoxide Dismutase); S88TT14065 (Oxygen)
[Em] Mês de entrada:1510
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141105
[St] Status:MEDLINE
[do] DOI:10.1038/srep06892


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[PMID]:25164344
[Au] Autor:Chen YC; Chen WT; Liu JC; Tsai LC; Cheng HL
[Ad] Endereço:Department of Biological Science and Technology, National Pingtung University of Science and Technology, 1, Shuehfu Rd., Neipu, Pingtung 91201, Taiwan, ROC.
[Ti] Título:A highly active beta-glucanase from a new strain of rumen fungus Orpinomyces sp.Y102 exhibits cellobiohydrolase and cellotriohydrolase activities.
[So] Source:Bioresour Technol;170:513-521, 2014 Oct.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A new strain of rumen fungus was isolated from Bos taurus, identified and designated Orpinomyces sp.Y102. A clone, celC7, isolated from the cDNA library of Orpinomyces sp.Y102, was predicted to encode a protein containing a signal peptide (Residues 1-17), an N-terminal dockerin-containing domain, and a C-terminal cellobiohydrolase catalytic domain of glycoside hydrolase family 6. CelC7 was insoluble when expressed in Escherichia coli. Deletion of 17 or 105 residues from the N-terminus significantly improved its solubility. The resulting enzymes, CelC7(-17) and CelC7(-105), were highly active to ß-glucan substrates and were stable between pH 5.0 and 11.0. CelC7(-105) worked as an exocellulase releasing cellobiose and cellotriose from acid-swollen Avicel and cellooligosaccharides, and displayed a Vmax of 6321.64µmole/min/mg and a Km of 2.18mg/ml to barley ß-glucan. Further, the crude extract of CelC7(-105) facilitated ethanol fermentation from cellulose. Thus, CelC7(-105) is a good candidate for industrial applications such as biofuel production.
[Mh] Termos MeSH primário: Bovinos/microbiologia
Celulases/metabolismo
Celulose 1,4-beta-Celobiosidase/metabolismo
Microbiologia Industrial/métodos
Neocallimastigales/enzimologia
Rúmen/microbiologia
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
Biocombustíveis
Western Blotting
Celulases/genética
Celulose 1,4-beta-Celobiosidase/genética
Cromatografia em Camada Delgada
Análise por Conglomerados
Primers do DNA/genética
Escherichia coli
Biblioteca Gênica
Dados de Sequência Molecular
Neocallimastigales/citologia
Neocallimastigales/genética
Filogenia
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Biofuels); 0 (DNA Primers); EC 3.2.1.- (Cellulases); EC 3.2.1.91 (Cellulose 1,4-beta-Cellobiosidase)
[Em] Mês de entrada:1506
[Cu] Atualização por classe:171009
[Lr] Data última revisão:
171009
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140829
[St] Status:MEDLINE


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[PMID]:24971799
[Au] Autor:Liggenstoffer AS; Youssef NH; Wilkins MR; Elshahed MS
[Ad] Endereço:Department of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater, OK 74074, United States.
[Ti] Título:Evaluating the utility of hydrothermolysis pretreatment approaches in enhancing lignocellulosic biomass degradation by the anaerobic fungus Orpinomyces sp. strain C1A.
[So] Source:J Microbiol Methods;104:43-8, 2014 Sep.
[Is] ISSN:1872-8359
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Members of the anaerobic fungi (Phylum Neocallimastigomycota) are efficient biomass degraders and represent promising agents for fuel and chemical production from lignocellulosic biomass. Pretreatment of lignocellulosic biomass is considered an unavoidable first step in enzyme-based saccharification schemes, but its necessity in any proposed anaerobic fungi-based schemes is still unclear. Here, we evaluated the effect of hydrothermal pretreatments on the extent of corn stover and switchgrass degradation by an anaerobic fungal isolate, Orpinomyces sp. strain C1A. Using a factorial experimental design, we evaluated the effect of three different temperatures (180, 190, and 200°C) and three hold times (5, 10, and 15min). Pretreated corn stover and switchgrass were more amenable to degradation by strain C1A when compared to untreated biomass, as evident by the higher proportion of plant biomass degraded compared to untreated controls. However, when factoring in the proportion of biomass lost during the pretreatment process (ranging between 25.78 and 58.92% in corn stover and 28.34 and 38.22% in switchgrass), hydrothermolysis provided negligible or negative improvements to the extent of corn stover and switchgrass degradation by strain C1A. Product analysis demonstrated a shift towards higher ethanol and lactate production and lower acetate production associated with increase in pretreatment severity, especially in switchgrass incubations. The results are in stark contrast to the requirement of pretreatment in enzyme-based schemes for biomass saccharification, and their implications on the potential utility of anaerobic fungi in biofuel and biochemical production are discussed.
[Mh] Termos MeSH primário: Biotecnologia/métodos
Lignina/metabolismo
Neocallimastigales/metabolismo
[Mh] Termos MeSH secundário: Anaerobiose
Biodegradação Ambiental
Biocombustíveis/análise
Biotecnologia/instrumentação
Etanol/análise
Etanol/metabolismo
Temperatura Alta
Hidrólise
Lignina/química
Zea mays/química
Zea mays/metabolismo
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Biofuels); 11132-73-3 (lignocellulose); 3K9958V90M (Ethanol); 9005-53-2 (Lignin)
[Em] Mês de entrada:1503
[Cu] Atualização por classe:140805
[Lr] Data última revisão:
140805
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140628
[St] Status:MEDLINE


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[PMID]:23709508
[Au] Autor:Youssef NH; Couger MB; Struchtemeyer CG; Liggenstoffer AS; Prade RA; Najar FZ; Atiyeh HK; Wilkins MR; Elshahed MS
[Ad] Endereço:Department of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater, Oklahoma, USA.
[Ti] Título:The genome of the anaerobic fungus Orpinomyces sp. strain C1A reveals the unique evolutionary history of a remarkable plant biomass degrader.
[So] Source:Appl Environ Microbiol;79(15):4620-34, 2013 Aug.
[Is] ISSN:1098-5336
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Anaerobic gut fungi represent a distinct early-branching fungal phylum (Neocallimastigomycota) and reside in the rumen, hindgut, and feces of ruminant and nonruminant herbivores. The genome of an anaerobic fungal isolate, Orpinomyces sp. strain C1A, was sequenced using a combination of Illumina and PacBio single-molecule real-time (SMRT) technologies. The large genome (100.95 Mb, 16,347 genes) displayed extremely low G+C content (17.0%), large noncoding intergenic regions (73.1%), proliferation of microsatellite repeats (4.9%), and multiple gene duplications. Comparative genomic analysis identified multiple genes and pathways that are absent in Dikarya genomes but present in early-branching fungal lineages and/or nonfungal Opisthokonta. These included genes for posttranslational fucosylation, the production of specific intramembrane proteases and extracellular protease inhibitors, the formation of a complete axoneme and intraflagellar trafficking machinery, and a near-complete focal adhesion machinery. Analysis of the lignocellulolytic machinery in the C1A genome revealed an extremely rich repertoire, with evidence of horizontal gene acquisition from multiple bacterial lineages. Experimental analysis indicated that strain C1A is a remarkable biomass degrader, capable of simultaneous saccharification and fermentation of the cellulosic and hemicellulosic fractions in multiple untreated grasses and crop residues examined, with the process significantly enhanced by mild pretreatments. This capability, acquired during its separate evolutionary trajectory in the rumen, along with its resilience and invasiveness compared to prokaryotic anaerobes, renders anaerobic fungi promising agents for consolidated bioprocessing schemes in biofuels production.
[Mh] Termos MeSH primário: Bovinos/microbiologia
Evolução Molecular
Genoma Fúngico
Neocallimastigales/genética
Rúmen/microbiologia
[Mh] Termos MeSH secundário: Adaptação Fisiológica
Animais
Biomassa
Bovinos/metabolismo
Celulose/metabolismo
Fezes/microbiologia
Fermentação
Masculino
Dados de Sequência Molecular
Neocallimastigales/classificação
Neocallimastigales/metabolismo
Filogenia
Rúmen/metabolismo
Análise de Sequência de DNA
Análise de Sequência de Proteína
Homologia de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
9004-34-6 (Cellulose)
[Em] Mês de entrada:1310
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130528
[St] Status:MEDLINE
[do] DOI:10.1128/AEM.00821-13



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