Base de dados : MEDLINE
Pesquisa : B01.650.520 [Categoria DeCS]
Referências encontradas : 29679 [refinar]
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  1 / 29679 MEDLINE  
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[PMID]:29381737
[Au] Autor:Wang Y; Zhang T; Song X; Zhang J; Dang Z; Pei X; Long Y
[Ad] Endereço:MOA Key Laboratory on Safety Assessment (Molecular) of Agri-GMO, Institute of Biotechnology, Chinese Academy of Agricultural Sciences, Beijing, China.
[Ti] Título:Identification and functional analysis of two alternatively spliced transcripts of ABSCISIC ACID INSENSITIVE3 (ABI3) in linseed flax (Linum usitatissimum L.).
[So] Source:PLoS One;13(1):e0191910, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Alternative splicing is a popular phenomenon in different types of plants. It can produce alternative spliced transcripts that encode proteins with altered functions. Previous studies have shown that one transcription factor, ABSCISIC ACID INSENSITIVE3 (ABI3), which encodes an important component in abscisic acid (ABA) signaling, is subjected to alternative splicing in both mono- and dicotyledons. In the current study, we identified two homologs of ABI3 in the genome of linseed flax. We screened two alternatively spliced flax LuABI3 transcripts, LuABI3-2 and LuABI3-3, and one normal flax LuABI3 transcript, LuABI3-1. Sequence analysis revealed that one of the alternatively spliced transcripts, LuABI3-3, retained a 6 bp intron. RNA accumulation analysis showed that all three transcripts were expressed during seed development, while subcellular localization and transgene experiments showed that LuABI3-3 had no biological function. The two normal transcripts, LuABI3-1 and LuABI3-2, are the important functional isoforms in flax and play significant roles in the ABA regulatory pathway during seed development, germination, and maturation.
[Mh] Termos MeSH primário: Processamento Alternativo
Linho/genética
Genes de Plantas
Proteínas de Plantas/genética
RNA Mensageiro/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Arabidopsis/genética
Germinação/genética
Proteínas de Plantas/química
Plantas Geneticamente Modificadas
Homologia de Sequência de Aminoácidos
Frações Subcelulares/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Plant Proteins); 0 (RNA, Messenger)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180131
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191910


  2 / 29679 MEDLINE  
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[PMID]:28461401
[Au] Autor:Hughes J; Hepworth C; Dutton C; Dunn JA; Hunt L; Stephens J; Waugh R; Cameron DD; Gray JE
[Ad] Endereço:Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom (J.H., C.D., J.A.D., L.H., J.E.G.).
[Ti] Título:Reducing Stomatal Density in Barley Improves Drought Tolerance without Impacting on Yield.
[So] Source:Plant Physiol;174(2):776-787, 2017 Jun.
[Is] ISSN:1532-2548
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The epidermal patterning factor (EPF) family of secreted signaling peptides regulate the frequency of stomatal development in model dicot and basal land plant species. Here, we identify and manipulate the expression of a barley ( ) ortholog and demonstrate that when overexpressed HvEPF1 limits entry to, and progression through, the stomatal development pathway. Despite substantial reductions in leaf gas exchange, barley plants with significantly reduced stomatal density show no reductions in grain yield. In addition, HvEPF1OE barley lines exhibit significantly enhanced water use efficiency, drought tolerance, and soil water conservation properties. Our results demonstrate the potential of manipulating stomatal frequency for the protection and optimization of cereal crop yields under future drier environments.
[Mh] Termos MeSH primário: Secas
Hordeum/fisiologia
Proteínas de Plantas/genética
Estômatos de Plantas/fisiologia
[Mh] Termos MeSH secundário: Desidratação
Regulação da Expressão Gênica de Plantas
Hordeum/genética
Hordeum/crescimento & desenvolvimento
Proteínas de Plantas/metabolismo
Plantas Geneticamente Modificadas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Proteins)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1104/pp.16.01844


  3 / 29679 MEDLINE  
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[PMID]:29371663
[Au] Autor:Shinozaki Y; Nicolas P; Fernandez-Pozo N; Ma Q; Evanich DJ; Shi Y; Xu Y; Zheng Y; Snyder SI; Martin LBB; Ruiz-May E; Thannhauser TW; Chen K; Domozych DS; Catalá C; Fei Z; Mueller LA; Giovannoni JJ; Rose JKC
[Ad] Endereço:Plant Biology Section, School of Integrative Plant Science, Cornell University, Ithaca, NY, 14853, USA.
[Ti] Título:High-resolution spatiotemporal transcriptome mapping of tomato fruit development and ripening.
[So] Source:Nat Commun;9(1):364, 2018 01 25.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Tomato (Solanum lycopersicum) is an established model for studying fruit biology; however, most studies of tomato fruit growth and ripening are based on homogenized pericarp, and do not consider the internal tissues, or the expression signatures of individual cell and tissue types. We present a spatiotemporally resolved transcriptome analysis of tomato fruit ontogeny, using laser microdissection (LM) or hand dissection coupled with RNA-Seq analysis. Regulatory and structural gene networks, including families of transcription factors and hormone synthesis and signaling pathways, are defined across tissue and developmental spectra. The ripening program is revealed as comprising gradients of gene expression, initiating in internal tissues then radiating outward, and basipetally along a latitudinal axis. We also identify spatial variations in the patterns of epigenetic control superimposed on ripening gradients. Functional studies elucidate previously masked regulatory phenomena and relationships, including those associated with fruit quality traits, such as texture, color, aroma, and metabolite profiles.
[Mh] Termos MeSH primário: Frutas/genética
Regulação da Expressão Gênica no Desenvolvimento
Regulação da Expressão Gênica de Plantas
Lycopersicon esculentum/genética
Transcriptoma
[Mh] Termos MeSH secundário: Frutas/crescimento & desenvolvimento
Frutas/ultraestrutura
Perfilação da Expressão Gênica/métodos
Redes Reguladoras de Genes
Lycopersicon esculentum/crescimento & desenvolvimento
Microscopia Eletrônica de Transmissão
Proteínas de Plantas/genética
Plantas Geneticamente Modificadas
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Plant Proteins)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180127
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02782-9


  4 / 29679 MEDLINE  
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[PMID]:29351549
[Au] Autor:García-Ruiz E; Loureiro Í; Farinós GP; Gómez P; Gutiérrez E; Sánchez FJ; Escorial MC; Ortego F; Chueca MC; Castañera P
[Ad] Endereço:Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Departamento de Protección Vegetal, Laboratorio de Malherbología, Madrid, Spain.
[Ti] Título:Weeds and ground-dwelling predators' response to two different weed management systems in glyphosate-tolerant cotton: A farm-scale study.
[So] Source:PLoS One;13(1):e0191408, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The use of glyphosate, as a post-emergence broad-spectrum herbicide in genetically modified glyphosate-tolerant (GT) cotton, supposes a big change in weed management programs with respect to a conventional regime. Thus, alterations in arable flora and arthropod fauna must be considered when evaluating their potential impacts. A 3-year farm-scale study was conducted in a 2-ha GT cotton crop, in southern Spain, to compare the effects of conventional and glyphosate herbicide regimes on weed abundance and diversity and their consequences for ground-dwelling predators. Surveys reveal that weed density was relatively low within all treatments with a few dominant species, with significantly higher weed densities and modifications of the floristic composition in glyphosate-treated plots that led to an increase in the abundance of Portulaca oleracea and to a reduction in plant diversity. The activity-density of the main predatory arthropod taxa (spiders, ground beetles, rove beetles and earwigs) varied among years, but no significant differences were obtained between conventional and glyphosate herbicide regimes. However, significant differences between treatments were obtained for ground beetles species richness and diversity, being higher under the glyphosate herbicide regime, and a positive correlation with weed density could be established for both parameters. The implications of these findings to weed control in GT cotton are discussed.
[Mh] Termos MeSH primário: Glicina/análogos & derivados
Gossypium/efeitos dos fármacos
Herbicidas/farmacologia
Plantas Daninhas/efeitos dos fármacos
Controle de Plantas Daninhas/métodos
[Mh] Termos MeSH secundário: Animais
Artrópodes/efeitos dos fármacos
Biodiversidade
Coleópteros/efeitos dos fármacos
Produtos Agrícolas/efeitos dos fármacos
Produtos Agrícolas/genética
Produtos Agrícolas/crescimento & desenvolvimento
Ecossistema
Cadeia Alimentar
Glicina/farmacologia
Gossypium/genética
Gossypium/crescimento & desenvolvimento
Resistência a Herbicidas/genética
Insetos/efeitos dos fármacos
Plantas Geneticamente Modificadas
Espanha
Aranhas/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Herbicides); 4632WW1X5A (glyphosate); TE7660XO1C (Glycine)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180120
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191408


  5 / 29679 MEDLINE  
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[PMID]:29253890
[Au] Autor:Patharkar OR; Gassmann W; Walker JC
[Ad] Endereço:Division of Biological Sciences and Interdisciplinary Plant Group, University of Missouri, Columbia, MO, United States of America.
[Ti] Título:Leaf shedding as an anti-bacterial defense in Arabidopsis cauline leaves.
[So] Source:PLoS Genet;13(12):e1007132, 2017 12.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Plants utilize an innate immune system to protect themselves from disease. While many molecular components of plant innate immunity resemble the innate immunity of animals, plants also have evolved a number of truly unique defense mechanisms, particularly at the physiological level. Plant's flexible developmental program allows them the unique ability to simply produce new organs as needed, affording them the ability to replace damaged organs. Here we develop a system to study pathogen-triggered leaf abscission in Arabidopsis. Cauline leaves infected with the bacterial pathogen Pseudomonas syringae abscise as part of the defense mechanism. Pseudomonas syringae lacking a functional type III secretion system fail to elicit an abscission response, suggesting that the abscission response is a novel form of immunity triggered by effectors. HAESA/HAESA-like 2, INFLORESCENCE DEFICIENT IN ABSCISSION, and NEVERSHED are all required for pathogen-triggered abscission to occur. Additionally phytoalexin deficient 4, enhanced disease susceptibility 1, salicylic acid induction-deficient 2, and senescence-associated gene 101 plants with mutations in genes necessary for bacterial defense and salicylic acid signaling, and NahG transgenic plants with low levels of salicylic acid fail to abscise cauline leaves normally. Bacteria that physically contact abscission zones trigger a strong abscission response; however, long-distance signals are also sent from distal infected tissue to the abscission zone, alerting the abscission zone of looming danger. We propose a threshold model regulating cauline leaf defense where minor infections are handled by limiting bacterial growth, but when an infection is deemed out of control, cauline leaves are shed. Together with previous results, our findings suggest that salicylic acid may regulate both pathogen- and drought-triggered leaf abscission.
[Mh] Termos MeSH primário: Arabidopsis/fisiologia
Folhas de Planta/genética
Folhas de Planta/fisiologia
[Mh] Termos MeSH secundário: Arabidopsis/genética
Proteínas de Arabidopsis/genética
Flores/genética
Genes de Plantas
Imunidade Inata
Inflorescência/genética
Mutação
Folhas de Planta/microbiologia
Plantas Geneticamente Modificadas
Proteínas Serina-Treonina Quinases/genética
Pseudomonas syringae/genética
Pseudomonas syringae/isolamento & purificação
Ácido Salicílico
Transdução de Sinais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Arabidopsis Proteins); EC 2.7.11.1 (Protein-Serine-Threonine Kinases); O414PZ4LPZ (Salicylic Acid)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171219
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1007132


  6 / 29679 MEDLINE  
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[PMID]:28926818
[Au] Autor:Duan M; Gu J; Wang X; Li Y; Zhang S; Yin Y; Zhang R
[Ad] Endereço:College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, China.
[Ti] Título:Effects of genetically modified cotton stalks on antibiotic resistance genes, intI1, and intI2 during pig manure composting.
[So] Source:Ecotoxicol Environ Saf;147:637-642, 2018 Jan.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Genetically modified (GM) cotton production generates a large yield of stalks and their disposal is difficult. In order to study the feasibility of using GM cotton stalks for composting and the changes that occur in antibiotic resistance genes (ARGs) during composting, we supplemented pig manure with GM or non-GM cotton stalks during composting and we compared their effects on the absolute abundances (AA) of intI1, intI2, and ARGs under the two treatments. The compost was mature after processing based on the germination index and C/N ratio. After composting, the AAs of ARGs, intI1, and intI2 were reduced by 41.7% and 45.0% in the non-GM and GM treatments, respectively. The ARG profiles were affected significantly by temperature and ammonia nitrogen. In addition, excluding tetC, GM cotton stalks had no significant effects on ARGs, intI1, and intI2 compared with the non-GM treatment (p < 0.05). Thus, similar to non-GM cotton stalks, GM cotton stalks can be used for aerobic composting with livestock manure, and the AAs of ARGs can be reduced. Furthermore, the results of this study provide a theoretical basis for the harmless utilization of GM cotton stalks.
[Mh] Termos MeSH primário: Compostagem/métodos
Resistência Microbiana a Medicamentos/genética
Genes Bacterianos
Gossypium/genética
Esterco/análise
Plantas Geneticamente Modificadas/genética
[Mh] Termos MeSH secundário: Animais
Antibacterianos/farmacologia
Sus scrofa
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Manure)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180223
[Lr] Data última revisão:
180223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170920
[St] Status:MEDLINE


  7 / 29679 MEDLINE  
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[PMID]:28926817
[Au] Autor:Chen Y; Yang Y; Zhu H; Romeis J; Li Y; Peng Y; Chen X
[Ad] Endereço:The State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
[Ti] Título:Safety of Bacillus thuringiensis Cry1C protein for Daphnia magna based on different functional traits.
[So] Source:Ecotoxicol Environ Saf;147:631-636, 2018 Jan.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Cry1C is a Bacillus thuringiensis (Bt) insecticidal protein and it can be produced by transgenic rice lines developed in China. Cladocera species are common aquatic arthropods that may be exposed to insecticidal proteins produced in Bt-transgenic plants through ingestion of pollen or crop residues in water. As the cladoceran Daphnia magna plays an important role in the aquatic food chain, it is important to assess the possible effects of Bt crops to this species. To evaluate the safety of the Cry1C protein for D. magna, individuals were exposed to different concentrations of purified Cry1C protein in M4 medium for 21 days. Potassium dichromate (K Cr O ), a known toxicant to D. magna, was added to M4 medium as a positive control treatment, and pure M4 medium was used as a negative control. Our results show that developmental, reproductive, and biochemical parameters of D. magna were not significantly different between Cry1C and negative control treatments but were significantly inhibited by the positive control. We thus conclude that D. magna is insensitive to Cry1C.
[Mh] Termos MeSH primário: Proteínas de Bactérias/toxicidade
Daphnia/efeitos dos fármacos
Endotoxinas/toxicidade
Proteínas Hemolisinas/toxicidade
Poluentes Químicos da Água/toxicidade
[Mh] Termos MeSH secundário: Animais
Proteínas de Bactérias/genética
China
Relação Dose-Resposta a Droga
Endotoxinas/genética
Proteínas Hemolisinas/genética
Oryza/genética
Oryza/metabolismo
Plantas Geneticamente Modificadas/genética
Plantas Geneticamente Modificadas/metabolismo
Testes de Toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Endotoxins); 0 (Hemolysin Proteins); 0 (Water Pollutants, Chemical); 0 (insecticidal crystal protein, Bacillus Thuringiensis)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180223
[Lr] Data última revisão:
180223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170920
[St] Status:MEDLINE


  8 / 29679 MEDLINE  
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[PMID]:29251473
[Au] Autor:Dong L; Meng Y; Wang J; Sun G
[Ti] Título:Effects of Transgenic Bt+CpTI cotton on the abundance and diversity of rhizosphere ammonia oxidizing bacteria and archaea.
[So] Source:J Environ Biol;37(5):881-8, 2016 09.
[Is] ISSN:0254-8704
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:Genetically modified crops (GMCs) hold great promise for improving agricultural output, but at the same time present challenges in terms of environmental safety assessment. Ammonia oxidizers, including ammonia oxidizing bacteria (AOB) and archaea (AOA), are very important functional microbial groups in nitrogen cycle. The abundance and diversity of AOA and AOB in the rhizosphere of genetically modified cotton (SGK321) and non-GM cotton (SY321) across growth stages were investigated using real time quantitative PCR (qPCR) and terminal restriction fragment length polymorphism (T-RFLP). Results showed that cotton genotype had a significant effect on the change in abundance of AOA and AOB, as indicated by amoA copy number. Variations in AOB abundance in rhizosphere of SY321 differed from those in SGK321. The number of AOB in the rhizosphere of SY321 fluctuated considerably: It dramatically decreased from 1.2?106 copies g-1 dry soil to 3?105 copies g-1 dry soil during the flowering stage and then increased to 1.1?106 copies g-1 and 1.5?106 copies g-1 at the belling and boll opening stages, respectively. However, abundance of AOB in the rhizosphere of SGK321 was relatively stable during all the stages of growth. The effect of SGK321 and SY321 on AOA number was quite similar to that of AOB: AOA abundance in SGK321 increased smoothly from 1.0 ?105 copies g-1 dry soil to 1.4?106 copies g-1 dry soil during growth, but that in SY321 fluctuated. Correspondence analysis (CA), canonical CA (CCA), and partial CCA (pCCA) of T-RFLP profiles of AOA and AOB showed that AOB community changed across growth stages in both cotton genotypes, and cotton genotype was the most important factor affecting the AOA community. In conclusion, the current findings indicated no adverse effect of GM cotton on functional microorganisms.
[Mh] Termos MeSH primário: Amônia/metabolismo
Archaea/metabolismo
Bactérias/metabolismo
Gossypium/genética
Microbiologia do Solo
[Mh] Termos MeSH secundário: Archaea/classificação
Bactérias/classificação
DNA Arqueal/genética
DNA Bacteriano/genética
Oxirredução
Raízes de Plantas
Plantas Geneticamente Modificadas
Solo
[Pt] Tipo de publicação:RESEARCH SUPPORT, NON-U.S. GOV'T; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Archaeal); 0 (DNA, Bacterial); 0 (Soil); 7664-41-7 (Ammonia)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171219
[St] Status:MEDLINE


  9 / 29679 MEDLINE  
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[PMID]:29293649
[Au] Autor:Dutt M; Zambon FT; Erpen L; Soriano L; Grosser J
[Ad] Endereço:Citrus Research and Education Center, University of Florida, Lake Alfred, Florida, United States of America.
[Ti] Título:Embryo-specific expression of a visual reporter gene as a selection system for citrus transformation.
[So] Source:PLoS One;13(1):e0190413, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The embryo-specific Dc3 gene promoter driving the VvMybA1 anthocyanin regulatory gene was used to develop a visual selection system for the genetic transformation of citrus. Agrobacterium-mediated transformation of cell suspension cultures resulted in the production of purple transgenic somatic embryos that could be easily separated from the green non-transgenic embryos. The somatic embryos produced phenotypically normal plants devoid of any visual purple coloration. These results were also confirmed using protoplast transformation. There was minimal gene expression in unstressed one-year-old transgenic lines. Cold and drought stress did not have any effect on gene expression, while exogenous ABA and NaCl application resulted in a minor change in gene expression in several transgenic lines. When gas exchange was measured in intact leaves, the transgenic lines were similar to controls under the same environment. Our results provide conclusive evidence for the utilization of a plant-derived, embryo-specific visual reporter system for the genetic transformation of citrus. Such a system could aid in the development of an all-plant, consumer-friendly GM citrus tree.
[Mh] Termos MeSH primário: Citrus/genética
Genes Reporter
Seleção Genética
Transformação Genética
[Mh] Termos MeSH secundário: Citrus/embriologia
Plantas Geneticamente Modificadas
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180103
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190413


  10 / 29679 MEDLINE  
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[PMID]:29229392
[Au] Autor:Zhou A; Sun H; Feng S; Zhou M; Gong S; Wang J; Zhang S
[Ad] Endereço:College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, China. Electronic address: aiminzhou@neau.edu.cn.
[Ti] Título:A novel cold-regulated gene from Phlox subulata, PsCor413im1, enhances low temperature tolerance in Arabidopsis.
[So] Source:Biochem Biophys Res Commun;495(2):1688-1694, 2018 01 08.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Low temperature stress adversely affects plant growth, development, and crop productivity. Analysis of the function of genes in the response of plants to low temperature stress is essential for understanding the mechanism of chilling and freezing tolerance. In this study, PsCor413im1, a novel cold-regulated gene isolated from Phlox subulata, was transferred to Arabidopsis to investigate its function under low temperature stress. Real-time quantitative PCR analysis revealed that PsCor413im1 expression was induced by cold and abscisic acid. Subcellular localization revealed that PsCor413im1-GFP fusion protein was localized to the periphery of the chloroplast, consistent with the localization of chloroplast inner membrane protein AtCor413im1, indicating that PsCor413im1 is a chloroplast membrane protein. Furthermore, the N-terminal of PsCor413im1 was determined to be necessary for its localization. Compared to the wild-type plants, transgenic plants showed higher germination and survival rates under cold and freezing stress. Moreover, the expression of AtCor15 in transgenic plants was higher than that in the wild-type plants under cold stress. Taken together, our results suggest that the overexpression of PsCor413im1 enhances low temperature tolerance in Arabidopsis.
[Mh] Termos MeSH primário: Aclimatação/genética
Arabidopsis/genética
Ericales/genética
Genes de Plantas
[Mh] Termos MeSH secundário: Aclimatação/fisiologia
Sequência de Aminoácidos
Arabidopsis/crescimento & desenvolvimento
Arabidopsis/fisiologia
Proteínas de Arabidopsis/genética
Proteínas de Arabidopsis/metabolismo
Temperatura Baixa
Ericales/fisiologia
Técnicas de Transferência de Genes
Filogenia
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
Plantas Geneticamente Modificadas
Proteínas Recombinantes de Fusão/genética
Proteínas Recombinantes de Fusão/metabolismo
Homologia de Sequência de Aminoácidos
Frações Subcelulares/metabolismo
Regulação para Cima
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (Plant Proteins); 0 (Recombinant Fusion Proteins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE



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