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Pesquisa : B01.650.940.800.150.150.575 [Categoria DeCS]
Referências encontradas : 15 [refinar]
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  1 / 15 MEDLINE  
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[PMID]:26781630
[Au] Autor:Atapaththu KS; Rashid MH; Asaeda T
[Ad] Endereço:Department of Environmental Science, Saitama University, 255 Shimo-okubo, Sakura-ku, Saitama, 338-8570, Japan.
[Ti] Título:Growth and Oxidative Stress of Brittlewort (Nitella pseudoflabellata) in Response to Cesium Exposure.
[So] Source:Bull Environ Contam Toxicol;96(3):347-53, 2016 Mar.
[Is] ISSN:1432-0800
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The present study evaluated the impact of cesium ((133)Cs) at four concentrations (0, 0.001, 0.01, and 0.1 mg L(-1)) on growth, concentrations of chlorophyll and carotenoid pigments, and oxidative stress responses in the charophyte, Nitella pseudoflabellata, over 30 days. Oxidative stress was quantified by measuring anti-oxidant enzyme activities and H2O2 content. When compared with the control, significantly elevated activity levels of the anti-oxidative enzymes ascorbic peroxidase, catalase and guaiacol peroxidase were observed at 0.1 mg L(-1) (all p < 0.05), even though the H2O2 level was not significantly elevated. Carotenoid and chlorophyll a and b pigment levels were significantly reduced (all p < 0.05) at Cs exposures of 0.01 and 0.1 mg L(-1). Photosynthetic efficiency (i.e., Fv/Fm) was significantly reduced (p < 0.05) at Cs concentrations ≥0.001 mg L(-1). Significant reduction (p < 0.05) of plant growth (i.e., shoot length) was also observed after 1 week of exposure at Cs concentrations ≥0.001 mg L(-1). Our results suggested that Cs exposure reduced plant growth and affected plant functioning via activating the defense mechanism against oxidative stress in Nitella.
[Mh] Termos MeSH primário: Césio/toxicidade
Nitella/efeitos dos fármacos
Estresse Oxidativo/efeitos dos fármacos
Poluentes Químicos da Água/toxicidade
[Mh] Termos MeSH secundário: Antioxidantes/metabolismo
Carotenoides/metabolismo
Catalase/metabolismo
Clorofila/metabolismo
Relação Dose-Resposta a Droga
Monitoramento Ambiental
Peróxido de Hidrogênio/metabolismo
Nitella/crescimento & desenvolvimento
Nitella/metabolismo
Oxirredução
Estresse Oxidativo/fisiologia
Peroxidase/metabolismo
Fotossíntese/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antioxidants); 0 (Water Pollutants, Chemical); 1406-65-1 (Chlorophyll); 1KSV9V4Y4I (Cesium); 36-88-4 (Carotenoids); BBX060AN9V (Hydrogen Peroxide); EC 1.11.1.- (guaiacol peroxidase); EC 1.11.1.6 (Catalase); EC 1.11.1.7 (Peroxidase); YF5Q9EJC8Y (chlorophyll a)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160120
[St] Status:MEDLINE
[do] DOI:10.1007/s00128-016-1736-4


  2 / 15 MEDLINE  
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[PMID]:26694322
[Au] Autor:Kikuchi K; Mochizuki O
[Ad] Endereço:Depertment of Bioengineering and Robotics, Graduated School of Engineering, Tohoku University, Sendai, Japan.
[Ti] Título:Diffusive Promotion by Velocity Gradient of Cytoplasmic Streaming (CPS) in Nitella Internodal Cells.
[So] Source:PLoS One;10(12):e0144938, 2015.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cytoplasmic streaming (CPS) is well known to assist the movement of nutrients, organelles and genetic material by transporting all of the cytoplasmic contents of a cell. CPS is generated by motility organelles that are driven by motor proteins near a membrane surface, where the CPS has been found to have a flat velocity profile in the flow field according to the sliding theory. There is a consistent mixing of contents inside the cell by CPS if the velocity gradient profile is flattened, which is not assisted by advection diffusion but is only supported by Brownian diffusion. Although the precise flow structure of the cytoplasm has an important role for cellular metabolism, the hydrodynamic mechanism of its convection has not been clarified. We conducted an experiment to visualise the flow of cytoplasm in Nitella cells by injecting tracer fluorescent nanoparticles and using a flow visualisation system in order to understand how the flow profile affects their metabolic system. We determined that the velocity field in the cytosol has an obvious velocity gradient, not a flattened gradient, which suggests that the gradient assists cytosolic mixing by Taylor-Aris dispersion more than by Brownian diffusion.
[Mh] Termos MeSH primário: Corrente Citoplasmática
Nitella/fisiologia
[Mh] Termos MeSH secundário: Hidrodinâmica
Proteínas Motores Moleculares/metabolismo
Nitella/ultraestrutura
Proteínas de Plantas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Molecular Motor Proteins); 0 (Plant Proteins)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151224
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0144938


  3 / 15 MEDLINE  
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[PMID]:23899506
[Au] Autor:Schallenberg-Rüdinger M; Lenz H; Polsakiewicz M; Gott JM; Knoop V
[Ad] Endereço:Abteilung Molekulare Evolution; Institut für Zelluläre und Molekulare Botanik; Universität Bonn; Bonn, Germany.
[Ti] Título:A survey of PPR proteins identifies DYW domains like those of land plant RNA editing factors in diverse eukaryotes.
[So] Source:RNA Biol;10(9):1549-56, 2013.
[Is] ISSN:1555-8584
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The pentatricopeptide repeat modules of PPR proteins are key to their sequence-specific binding to RNAs. Gene families encoding PPR proteins are greatly expanded in land plants where hundreds of them participate in RNA maturation, mainly in mitochondria and chloroplasts. Many plant PPR proteins contain additional carboxyterminal domains and have been identified as essential factors for specific events of C-to-U RNA editing, which is abundant in the two endosymbiotic plant organelles. Among those carboxyterminal domain additions to plant PPR proteins, the so-called DYW domain is particularly interesting given its similarity to cytidine deaminases. The frequency of organelle C-to-U RNA editing and the diversity of DYW-type PPR proteins correlate well in plants and both were recently identified outside of land plants, in the protist Naegleria gruberi. Here we present a systematic survey of PPR protein genes and report on the identification of additional DYW-type PPR proteins in the protists Acanthamoeba castellanii, Malawimonas jakobiformis, and Physarum polycephalum. Moreover, DYW domains were also found in basal branches of multi-cellular lineages outside of land plants, including the alga Nitella flexilis and the rotifers Adineta ricciae and Philodina roseola. Intriguingly, the well-characterized and curious patterns of mitochondrial RNA editing in the slime mold Physarum also include examples of C-to-U changes. Finally, we identify candidate sites for mitochondrial RNA editing in Malawimonas, further supporting a link between DYW-type PPR proteins and C-to-U editing, which may have remained hitherto unnoticed in additional eukaryote lineages.
[Mh] Termos MeSH primário: Embriófitas/genética
Eucariotos
Proteínas de Plantas/metabolismo
Edição de RNA
Proteínas de Ligação a RNA/metabolismo
[Mh] Termos MeSH secundário: Acanthamoeba castellanii/genética
Acanthamoeba castellanii/metabolismo
Embriófitas/metabolismo
Naegleria/genética
Nitella/genética
Nitella/metabolismo
Organelas/genética
Organelas/metabolismo
Filogenia
Physarum polycephalum/genética
Physarum polycephalum/metabolismo
Proteínas de Plantas/genética
Células Procarióticas/metabolismo
Estrutura Terciária de Proteína
Proteínas de Ligação a RNA/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Proteins); 0 (RNA-Binding Proteins)
[Em] Mês de entrada:1505
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130801
[St] Status:MEDLINE
[do] DOI:10.4161/rna.25755


  4 / 15 MEDLINE  
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[PMID]:22118365
[Au] Autor:Foissner I; Wasteneys GO
[Ad] Endereço:Cell Biology/Plant Physiology, University of Salzburg, Austria. ilse.foissner@sbg.ac.at
[Ti] Título:The characean internodal cell as a model system for studying wound healing.
[So] Source:J Microsc;247(1):10-22, 2012 Jul.
[Is] ISSN:1365-2818
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This work describes the characean internodal cell as a model system for the study of wound healing and compares wounds induced by certain chemicals and UV irradiation with wounds occurring in the natural environment. We review the existing literature and define three types of wound response: (1) cortical window formation characterised by disassembly of microtubules, transient inhibition of actin-dependent cytoplasmic streaming and chloroplast detachment, (2) fibrillar wound walls characterised by exocytosis of vesicles carrying wall polysaccharides and membrane-bound cellulose synthase complexes coupled with endocytosis of surplus membrane and (3) amorphous, callose- and membrane-containing wound walls characterised by exocytosis of vesicles and endoplasmic reticulum cisternae in the absence of membrane recycling. We hypothesize that these three wound responses reflect the extent of damage, probably Ca(2+) influx, and that the secretion of Ca(2+) -loaded endoplasmic reticulum cisternae is an emergency reaction in case of severe Ca(2+) load. Microtubules are not required for wound healing but their disassembly could have a signalling function. Transient reorganisation of the actin cytoskeleton into a meshwork of randomly oriented filaments is required for the migration of wound wall forming organelles, just as occurs in tip-growing plant cells. New data presented in this study show that during the deposition of an amorphous wound wall numerous actin rings are present, which may indicate specific ion fluxes and/or a storage form for actin. In addition, we present new evidence for the exocytosis of FM1-43-stained organelles, putative endosomes, required for plasma membrane repair during wound healing. Finally, we show that quickly growing fibrillar wound walls, even when deposited in the absence of microtubules, have a highly ordered helical structure of consistent handedness comprised of cellulose microfibrils.
[Mh] Termos MeSH primário: Chara/citologia
Chara/fisiologia
Nitella/citologia
Nitella/fisiologia
Células Vegetais/metabolismo
Cicatrização
[Mh] Termos MeSH secundário: Actinas/metabolismo
Citoesqueleto/metabolismo
Células Vegetais/química
Células Vegetais/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Actins)
[Em] Mês de entrada:1210
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:111129
[St] Status:MEDLINE
[do] DOI:10.1111/j.1365-2818.2011.03572.x


  5 / 15 MEDLINE  
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[PMID]:21668600
[Au] Autor:Klima A; Foissner I
[Ad] Endereço:Division of Plant Physiology, Department of Cell Biology, University of Salzburg, Hellbrunnerstrasse 34, Salzburg, Austria.
[Ti] Título:Actin-dependent deposition of putative endosomes and endoplasmic reticulum during early stages of wound healing in characean internodal cells.
[So] Source:Plant Biol (Stuttg);13(4):590-601, 2011 Jul.
[Is] ISSN:1438-8677
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:We investigated the behaviour of organelles stained with FM1-43 (putative endosomes) and/or LysoTracker Red (LTred; acidic compartments) and of the endoplasmic reticulum (ER) during healing of puncture and UV-induced wounds in internodal cells of Nitella flexilis and Chara corallina. Immediately after puncture, wounds were passively sealed with a plug of solid vacuolar inclusions, onto which a bipartite wound wall was actively deposited. The outer, callose-containing amorphous layer consisted of remnants of FM1-43- and LTred-labelled organelles, ER cisternae and polysaccharide-containing secretory vesicles, which became deposited in the absence of membrane retrieval (compound exocytosis). During formation of the inner cellulosic layer, exocytosis of secretory vesicles with the newly formed plasma membrane is coupled to endocytosis via coated vesicles. Migration of FM1-43- and LTred-stained organelles, ER and secretory vesicles towards the cell cortex and deposition of a bipartite wound wall could also be induced by spot-like irradiation with ultraviolet light. Cytochalasin D reversibly inhibited the accumulation and deposition of organelles. Our study indicates that active actin-dependent deposition of putative recycling endosomes is required for wound healing (plasma membrane repair) and supports the hypothesis that deposition of ER cisternae helps to restore wounding-disturbed Ca(2+) metabolism.
[Mh] Termos MeSH primário: Actinas/metabolismo
Estruturas Celulares/metabolismo
Chara/fisiologia
Retículo Endoplasmático/metabolismo
Endossomos/metabolismo
Nitella/fisiologia
Doenças das Plantas
[Mh] Termos MeSH secundário: Cálcio/metabolismo
Membrana Celular/metabolismo
Parede Celular/metabolismo
Celulose/metabolismo
Chara/efeitos dos fármacos
Chara/efeitos da radiação
Citocalasina D/farmacologia
Endocitose
Exocitose
Nitella/efeitos dos fármacos
Nitella/efeitos da radiação
Polissacarídeos/metabolismo
Compostos de Piridínio
Compostos de Amônio Quaternário
Vesículas Secretórias/metabolismo
Raios Ultravioleta/efeitos adversos
Vacúolos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Actins); 0 (FM1 43); 0 (Polysaccharides); 0 (Pyridinium Compounds); 0 (Quaternary Ammonium Compounds); 22144-77-0 (Cytochalasin D); 9004-34-6 (Cellulose); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1112
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110615
[St] Status:MEDLINE
[do] DOI:10.1111/j.1438-8677.2010.00413.x


  6 / 15 MEDLINE  
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[PMID]:19157705
[Au] Autor:Gomes PI; Asaeda T
[Ad] Endereço:Department of Environmental Science and Technology, Saitama University, 255 Shimo-okubo, Sakura-ku, Saitama 338-8770, Japan.
[Ti] Título:Phycoremediation of Chromium (VI) by Nitella and impact of calcium encrustation.
[So] Source:J Hazard Mater;166(2-3):1332-8, 2009 Jul 30.
[Is] ISSN:1873-3336
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:This article discusses the applicability of the Charophyte, Nitella pseudoflabellata in the remediation of Cr (VI) contaminated waters at different calcifying potentials. Its growth was found to be positively correlated with Ca in water (CaW), but marginally significant in the presence of Cr (VI) in water (CrW). High CaW resulted in calcite encrustation on the plant cell wall. CaW was found to be aiding Cr (VI) fixation in the long run, as this correlated positively with both CaW and CrW. However, Ca interfered with passive Cr (VI) accumulation in live plant matter at low CrW concentrations (1mg/g Cr dry weight of plant. Cr (VI) concentrations greater than 0.4 mg/L were too toxic, showing maximum quantum efficiency of PSII photochemistry (F(v)/F(m)) values<0.63. The opposite was noticed (F(v)/F(m)>0.76) when Cr (VI) was less than 0.2mg/L. Elongation curve patterns based on shoot lengths showed similar scenarios. In all cases high CaW units with calcite encrustation found to be least affected by Cr (VI) toxicity. Optimum remediation was obtained using a combination of high Ca and Cr (VI) in the case of passive (short-term) operation and low Ca and Cr (VI) for active (long-term) operation. Under the passive scenario, plants accumulated above 1.2mg/g Cr dry weight whereas in the active case, accumulation was 0.8 mg/g Cr dry weight. We conclude that Nitella-mediated Cr (VI) remediation is a promising technique within the range and conditions investigated.
[Mh] Termos MeSH primário: Biodegradação Ambiental
Cálcio/química
Cromo/metabolismo
Nitella/metabolismo
[Mh] Termos MeSH secundário: Carbonato de Cálcio
Cromo/toxicidade
Minerais
Nitella/fisiologia
Complexo de Proteínas do Centro de Reação Fotossintética/efeitos dos fármacos
Complexo de Proteína do Fotossistema II/efeitos dos fármacos
Poluentes Químicos da Água/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Minerals); 0 (Photosynthetic Reaction Center Complex Proteins); 0 (Photosystem II Protein Complex); 0 (Water Pollutants, Chemical); 0R0008Q3JB (Chromium); H0G9379FGK (Calcium Carbonate); SY7Q814VUP (Calcium)
[Em] Mês de entrada:0908
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:090123
[St] Status:MEDLINE
[do] DOI:10.1016/j.jhazmat.2008.12.055


  7 / 15 MEDLINE  
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[PMID]:19137584
[Au] Autor:Foissner I; Menzel D; Wasteneys GO
[Ad] Endereço:Department of Cell Biology, University of Salzburg, Austria. ilse.foissner@sbg.ac.at
[Ti] Título:Microtubule-dependent motility and orientation of the cortical endoplasmic reticulum in elongating characean internodal cells.
[So] Source:Cell Motil Cytoskeleton;66(3):142-55, 2009 Mar.
[Is] ISSN:1097-0169
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Motility of the endoplasmic reticulum (ER) is predominantly microtubule- dependent in animal cells but thought to be entirely actomyosin-dependent in plant cells. Using live cell imaging and transmission electron microscopy to examine ER motility and structural organization in giant internodal cells of characean algae, we discovered that at the onset of cell elongation, the cortical ER situated near the plasma membrane formed a tight meshwork of predominantly transverse ER tubules that frequently coaligned with microtubules. Microtubule depolymerization increased mesh size and decreased the dynamics of the cortical ER. In contrast, perturbing the cortical actin array with cytochalasins did not affect the transverse orientation but decreased mesh size and increased ER dynamics. Our data suggest that myosin-dependent ER motility is confined to the ER strands in the streaming endoplasm, while the more sedate cortical ER uses microtubule-based mechanisms for organization and motility during early stages of cell elongation. We show further that the ER has an inherent, NEM-sensitive dynamics which can be altered via interaction with the cytoskeleton and that tubule formation and fusion events are cytoskeleton-independent.
[Mh] Termos MeSH primário: Movimento Celular/fisiologia
Corrente Citoplasmática/fisiologia
Citoesqueleto/metabolismo
Retículo Endoplasmático/metabolismo
Microtúbulos/metabolismo
Nitella/metabolismo
[Mh] Termos MeSH secundário: Actinas/metabolismo
Inibidores da Angiogênese/farmacologia
Movimento Celular/efeitos dos fármacos
Células Cultivadas
Cloroplastos/metabolismo
Citocalasina D/farmacologia
Citocalasinas/farmacologia
Citoplasma/metabolismo
Corrente Citoplasmática/efeitos dos fármacos
Retículo Endoplasmático/efeitos dos fármacos
Fluorescência
Micotoxinas
Miosinas/metabolismo
Inibidores da Síntese de Ácido Nucleico/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Actins); 0 (Angiogenesis Inhibitors); 0 (Cytochalasins); 0 (Mycotoxins); 0 (Nucleic Acid Synthesis Inhibitors); 22144-77-0 (Cytochalasin D); 36011-19-5 (cytochalasin E); EC 3.6.4.1 (Myosins)
[Em] Mês de entrada:0904
[Cu] Atualização por classe:091119
[Lr] Data última revisão:
091119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:090113
[St] Status:MEDLINE
[do] DOI:10.1002/cm.20337


  8 / 15 MEDLINE  
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[PMID]:17477057
[Au] Autor:Zherelova OM; Kabanova NV; Kazachenko VN; Chailakhian LM
[Ti] Título:[Interaction of melittin with ion channels of excitable membranes].
[So] Source:Biofizika;52(2):295-300, 2007 Mar-Apr.
[Is] ISSN:0006-3029
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:The effect of the neurotoxin melittin on the activation of ion channels of excitable membrane, the plasmalemma of Characeae algae cells, isolated membrane patches of neurons of mollusc L. stagnalis and Vero cells was studied by the method of intracellular perfusion and the patch-clamp technique in inside-out configuration. It was shown that melittin disturbs the conductivity of plasmalemma and modifieds Ca(2+)-channels of plant membrane. The leakage current that appears by the action of melittin can be restored by substituting calmodulin for melittin. Melittin modifies K(+)-channels of animal cell membrane by disrupting the phospholipid matrix and forms conductive structures in the membrane by interacting with channel proteins, which is evidenced by the appearance of additional ion channels.
[Mh] Termos MeSH primário: Canais de Cálcio/efeitos dos fármacos
Membrana Celular/efeitos dos fármacos
Meliteno/farmacologia
Canais de Potássio/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Cercopithecus aethiops
Potenciais da Membrana/efeitos dos fármacos
Moluscos/citologia
Neurônios/efeitos dos fármacos
Nitella/citologia
Técnicas de Patch-Clamp
Células Vero
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Calcium Channels); 0 (Potassium Channels); 20449-79-0 (Melitten)
[Em] Mês de entrada:0707
[Cu] Atualização por classe:070504
[Lr] Data última revisão:
070504
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:070505
[St] Status:MEDLINE


  9 / 15 MEDLINE  
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[PMID]:17327257
[Au] Autor:Foissner I; Wasteneys GO
[Ad] Endereço:Department of Cell Biology, University of Salzburg, Salzburg, Austria. ilse.foissner@sbg.ac.at
[Ti] Título:Wide-ranging effects of eight cytochalasins and latrunculin A and B on intracellular motility and actin filament reorganization in characean internodal cells.
[So] Source:Plant Cell Physiol;48(4):585-97, 2007 Apr.
[Is] ISSN:0032-0781
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Numerous forms of cytochalasins have been identified and, although they share common biological activity, they may differ considerably in potency. We investigated the effects of cytochalasins A, B, C, D, E, H and J and dihydrocytochalasin B in an ideal experimental system for cell motility, the giant internodal cells of the characean alga Nitella pseudoflabellata. Cytochalasins D (60 microM) and H (30 microM) were found to be most suited for fast and reversible inhibition of actin-based motility, while cytochalasins A and E arrested streaming at lower concentrations but irreversibly. We observed no clear correlation between the ability of cytochalasins to inhibit motility and the actual disruption of the subcortical actin bundle tracks on which myosin-dependent motility occurs. Indeed, the actin bundles remained intact at the time of streaming cessation and disassembled only after one to several days' treatment. Even when applied at concentrations lower than that required to inhibit cytoplasmic streaming, all of the cytochalasins induced reorganization of the more labile cortical actin filaments into actin patches, swirling clusters or short rods. Latrunculins A and B arrested streaming only after disrupting the subcortical actin bundles, a process requiring relatively high concentrations (200 microM) and very long treatment periods of >1 d. Latrunculins, however, worked synergistically with cytochalasins. A 1 h treatment with 15 nM latrunculin A and 4 microM cytochalasin D induced reversible fragmentation of subcortical actin bundles and arrested cytoplasmic streaming. Our findings provide insights into the mechanisms by which cytochalasins and latrunculins interfere with characean actin to inhibit motility.
[Mh] Termos MeSH primário: Actinas/metabolismo
Compostos Bicíclicos Heterocíclicos com Pontes/farmacologia
Movimento Celular/efeitos dos fármacos
Citocalasinas/farmacologia
Nitella/efeitos dos fármacos
Tiazolidinas/farmacologia
[Mh] Termos MeSH secundário: Nitella/citologia
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Actins); 0 (Bridged Bicyclo Compounds, Heterocyclic); 0 (Cytochalasins); 0 (Thiazolidines); LW7U308U7U (latrunculin B); SRQ9WWM084 (latrunculin A)
[Em] Mês de entrada:0706
[Cu] Atualização por classe:161124
[Lr] Data última revisão:
161124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:070301
[St] Status:MEDLINE


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[PMID]:16521543
[Au] Autor:Bazarova BB; Itigilova MTs
[Ti] Título:[Long-term production dynamics of aquatic vegetation in the Arakhlei Lake (Eastern Transbaikalia)].
[So] Source:Izv Akad Nauk Ser Biol;(1):81-5, 2006 Jan-Feb.
[Is] ISSN:1026-3470
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:Long-term data on aquatic vegetation production in the mesotrophic Arakhlei Lake was comparatively analyzed. The results demonstrated that the production dynamics of aquatic vegetation communities depend on the water-level conditions of the lake. The data on the succession of dominant vegetation communities are given: Chara sp. and Ceratophyllum demersum --> C. demersum and Lemna trisulca. At the same time, the increased proportion of C. demersum and L. trisulca pointed to an increased anthropogenic stress on the lake.
[Mh] Termos MeSH primário: Araceae/crescimento & desenvolvimento
Chara/crescimento & desenvolvimento
Conservação dos Recursos Naturais
Água Doce
Magnoliopsida/crescimento & desenvolvimento
Nitella/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Ecossistema
Federação Russa
Sibéria
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:0604
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:060309
[St] Status:MEDLINE



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