Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.100.157.600 [Categoria DeCS]
Referências encontradas : 194 [refinar]
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[PMID]:28117590
[Au] Autor:Zhang M; Wu W; Ren Y; Li X; Tang Y; Min T; Lai F; Wu H
[Ad] Endereço:College of Food Sciences and Engineering, South China University of Technology , Guangzhou, Guangdong 510640, China.
[Ti] Título:Structural Characterization of a Novel Polysaccharide from Lepidium meyenii (Maca) and Analysis of Its Regulatory Function in Macrophage Polarization in Vitro.
[So] Source:J Agric Food Chem;65(6):1146-1157, 2017 Feb 15.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In our previous study, three novel polysaccharides, named MC-1, MC-2, and MC-3, were separated from the roots of maca (Lepidium meyenii), which is a food source from the Andes region. The structural information and immunomodulatory activity of MC-1 were then investigated. The structure and activity of MC-2 are still unknown. In this study, structural characterization revealed that MC-2 has an average molecular weight of 9.83 kDa and is composed of arabinose (20.9%), mannose (4.5%), glucose (71.9%), and galactose (2.7%). The main linkage types of MC-2 were proven to be (1→5)-α-l-Ara, (1→3)-α-l-Man, (1→)-α-d-Glc, (1→4)-α-d-Glc, (1→6)-α-d-Glc, and (1→6)-ß-d-Gal by methylation and NMR analyses. Congo red assay showed that MC-2 possesses a triple-helix conformation. Immunostimulating assays indicated that MC-2 could induce M1 polarization of original macrophages and convert M2 macrophages into M1 phenotype. Although MC-2 could not shift M1 macrophages into M2, it could still inhibit inflammatory reactions induced by lipopolysaccharide. Furthermore, Toll-like receptor 2, tTll-like receptor 4, complement receptor 3, and mannose receptor were confirmed as the membrane receptors for MC-2 on macrophages. These results indicate that MC-2 could potentially be used toward hypoimmunity and tumor therapies.
[Mh] Termos MeSH primário: Lepidium/química
Macrófagos/efeitos de drogas
Polissacarídeos/química
Polissacarídeos/farmacologia
[Mh] Termos MeSH secundário: Animais
Configuração de Carboidratos
Linhagem Celular
Fatores Imunológicos/química
Fatores Imunológicos/farmacologia
Interleucina-10/metabolismo
Interleucina-6/metabolismo
Macrófagos/metabolismo
Espectroscopia de Ressonância Magnética
Metilação
Camundongos
Peso Molecular
Monossacarídeos/análise
Óxido Nítrico Sintase Tipo II/metabolismo
Espectroscopia Infravermelho Transformada de Fourier
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (IL10 protein, mouse); 0 (Immunologic Factors); 0 (Interleukin-6); 0 (Monosaccharides); 0 (Polysaccharides); 0 (interleukin-6, mouse); 130068-27-8 (Interleukin-10); EC 1.14.13.39 (Nitric Oxide Synthase Type II); EC 1.14.13.39 (Nos2 protein, mouse)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170602
[Lr] Data última revisão:
170602
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170124
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.6b05218


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[PMID]:28343387
[Au] Autor:Pagnotta E; Agerbirk N; Olsen CE; Ugolini L; Cinti S; Lazzeri L
[Ad] Endereço:Council for Agricultural Research and Economics, Research Centre for Industrial Crops, CREA-CIN , via di Corticella 133, 40128 Bologna, Italy.
[Ti] Título:Hydroxyl and Methoxyl Derivatives of Benzylglucosinolate in Lepidium densiflorum with Hydrolysis to Isothiocyanates and non-Isothiocyanate Products: Substitution Governs Product Type and Mass Spectral Fragmentation.
[So] Source:J Agric Food Chem;65(15):3167-3178, 2017 Apr 19.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A system of benzylic glucosinolates was found and characterized in common pepperweed, Lepidium densiflorum Schrad. The major glucosinolate was the novel 4-hydroxy-3,5-dimethoxybenzylglucosinolate (3,5-dimethoxysinalbin), present at high levels in seeds, leaves, and roots. Medium-level glucosinolates were 3,4-dimethoxybenzylglucosinolate and 3,4,5-trimethoxybenzylglucosinolate. Minor glucosinolates included benzylglucosinolate, 3-hydroxy- and 3-methoxybenzylglucosinolate, 4-hydroxybenzylglucosinolate (sinalbin), the novel 4-hydroxy-3-methoxybenzylglucosinolate (3-methoxysinalbin), and indole-type glucosinolates. A biosynthetic connection is suggested. NMR, UV, and ion trap MS/MS spectral data are reported, showing contrasting MS fragmentation of p-hydroxyls and p-methoxyls. Additional investigations by GC-MS focused on glucosinolate hydrolysis products. Whereas glucosinolates generally yielded isothiocyanates, the dominating 3,5-dimethoxysinalbin with a free p-hydroxyl group produced the corresponding alcohol and syringaldehyde (4-hydroxy-3,5-dimethoxybenzaldehyde). After thermal deactivation of the endogenous myrosinase enzyme, massive accumulation of the corresponding nitrile was detected. This case study points out how non-isothiocyanate glucosinolate hydrolysis products are prevalent in nature and of interest in both plant-pathogen interactions and human health.
[Mh] Termos MeSH primário: Isotiocianatos/química
Lepidium/química
Extratos Vegetais/química
Tiocianatos/química
Tioglucosídeos/química
[Mh] Termos MeSH secundário: Hidrólise
Estrutura Molecular
Folhas de Planta/química
Sementes/química
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Isothiocyanates); 0 (Plant Extracts); 0 (Thiocyanates); 0 (Thioglucosides); 0 (benzylglucosinolic acid); 3129-90-6 (isothiocyanic acid)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170601
[Lr] Data última revisão:
170601
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170327
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b00529


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[PMID]:28212012
[Au] Autor:Zhou M; Ma HY; Liu ZH; Yang GY; Du G; Ye YQ; Li GP; Hu QF
[Ad] Endereço:Key Laboratory of Chemistry in Ethnic Medicinal Resources, State Ethnic Affairs Commission and Ministry of Education, Yunnan Minzu University , Kunming 650031, Yunnan, People's Republic of China.
[Ti] Título:(+)-Meyeniins A-C, Novel Hexahydroimidazo[1,5-c]thiazole Derivatives from the Tubers of Lepidium meyenii: Complete Structural Elucidation by Biomimetic Synthesis and Racemic Crystallization.
[So] Source:J Agric Food Chem;65(9):1887-1892, 2017 Mar 08.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:(+)-Meyeniins A-C (1-3), a novel class of sulfur-containing hexahydroimidazo[1,5-c]thiazole derivatives, were isolated from the tubers of Lepidium meyenii (maca) cultivated in Lijiang, Yunnan province, China. Guided by their biosynthetic hypothesis, a stereocontrolled biomimetic synthesis of meyeniins A-C and their individual enantiomers was efficiently accomplished by a combination of a condensation reaction and Edman degradation. The formation of high-quality crystals for X-ray crystallography occurred much more readily from a racemic mixture of (±)-meyeniin A than with the single enantiomer alone in this case. These extensive strategies, combined with circular dichroism (CD) spectra, allowed the complete structural assignments of (+)-meyeniins A-C. Among them, (+)-meyeniin A showed moderate selective cytotoxicities against the HL-60, A549 and MCF-7 human cell lines with IC values of 14.41, 32.22, and 33.14 µM, respectively. To some extent, these findings support traditional applications of maca as healthy nutritional supplements or functional foods for cancer prevention.
[Mh] Termos MeSH primário: Imidazóis/química
Lepidium/química
Extratos Vegetais/química
Tubérculos/química
Tiazóis/química
[Mh] Termos MeSH secundário: Biomimética
Linhagem Celular Tumoral
Proliferação de Células/efeitos de drogas
Cristalização
Cristalografia por Raios X
Humanos
Imidazóis/isolamento & purificação
Imidazóis/farmacologia
Estrutura Molecular
Extratos Vegetais/isolamento & purificação
Extratos Vegetais/farmacologia
Tiazóis/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Imidazoles); 0 (Plant Extracts); 0 (Thiazoles); 0 (imidazolone)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170531
[Lr] Data última revisão:
170531
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170217
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.6b05805


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[PMID]:28231388
[Au] Autor:Tang W; Jin L; Xie L; Huang J; Wang N; Chu B; Dai X; Liu Y; Wang R; Zhang Y
[Ad] Endereço:College of Biosystems Engineering and Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang Engineering Center for Food Technology and Equipment, Zhejiang Univ, Hangzhou, Zhejiang, 310058, P.R., China.
[Ti] Título:Structural Characterization and Antifatigue Effect In Vivo of Maca (Lepidium meyenii Walp) Polysaccharide.
[So] Source:J Food Sci;82(3):757-764, 2017 Mar.
[Is] ISSN:1750-3841
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Maca (Lepidium meyenii Walp) polysaccharides (MP) with purity of 99.2% were obtained to investigate their structural characteristics and antifatigue effect in vivo. The physicochemical properties of MP were analyzed through high-performance gel filtration chromatography, IR, monosaccharide composition, methylation, GC-MS, and NMR analyses. The antifatigue effect of MP was evaluated by using a mouse weight-loaded swimming model. MP is an acidic heteropolysaccharide with an average molecular weight (M ) of 793.5 kDa. It is composed of D-GalA: D-Glc: L-Ara: D-Man: D-Gal: L-Rha = 35.07:29.98:16.98:13.01:4.21:0.75 (mol, %). The findings revealed that MP contained ß-1,3-Galp(A), ß-1,3-Glcp, and α-1, 3-Manp linked alternatingly to form a backbone (5:4:1). MP (above mid-dosage 50 mg/kg bw/d) could effectively elongate swimming durations and accelerate average swimming speeds (within the 1st 5 min) of mice (P < 0.05) and improve the serous biochemical parameters of mice. Compared with the control model, high-dosage (100 mg/kg bw/d) MP treatment could significantly enhance glutathione peroxidase and creatine kinase activities (P < 0.05) and decreased lactate dehydrogenase activity (P < 0.01). High-dosage MP could significantly reduce the levels of blood urea nitrogen, lactic acid, and malondialdehyde (P < 0.05). MP is an acidic polysaccharide with a high D-GalA content, which could be responsible for the antifatigue effect of maca.
[Mh] Termos MeSH primário: Fadiga
Lepidium/química
Resistência Física/efeitos de drogas
Extratos Vegetais/farmacologia
Polissacarídeos/farmacologia
[Mh] Termos MeSH secundário: Animais
Nitrogênio da Ureia Sanguínea
Creatina Quinase/metabolismo
Fadiga/prevenção & controle
Feminino
Glutationa Peroxidase/metabolismo
L-Lactato Desidrogenase/metabolismo
Ácido Láctico/sangue
Espectroscopia de Ressonância Magnética
Masculino
Malondialdeído/sangue
Camundongos Endogâmicos ICR
Estrutura Molecular
Peso Molecular
Extratos Vegetais/química
Polissacarídeos/química
Natação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Extracts); 0 (Polysaccharides); 33X04XA5AT (Lactic Acid); 4Y8F71G49Q (Malondialdehyde); EC 1.1.1.27 (L-Lactate Dehydrogenase); EC 1.11.1.9 (Glutathione Peroxidase); EC 2.7.3.2 (Creatine Kinase)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170522
[Lr] Data última revisão:
170522
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170223
[St] Status:MEDLINE
[do] DOI:10.1111/1750-3841.13619


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[PMID]:28350346
[Au] Autor:Wang S; Shi P; Qu L; Ruan J; Yang S; Yu H; Zhang Y; Wang T
[Ad] Endereço:Tianjin State Key Laboratory of Modern Chinese Medicine, 312 Anshanxi Road, Nankai District, Tianjin 300193, China. 15122587883@163.com.
[Ti] Título:Bioactive Constituents Obtained from the Seeds of Lepidium apetalum Willd.
[So] Source:Molecules;22(4), 2017 Mar 28.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Three new compounds, apetalumosides C1 ( ), D ( ), and 1-thio--d-glucopyranosyl(1→1)-1-thio-α-d-glucopyranoside ( ), together with twenty-two known ones (4-25) were obtained from the seeds of Willd. Among the known isolates, - , - , - , and were obtained from the genus for the first time; , , , and - were isolated from the species for the first time. Meanwhile, the NMR data of 16 was first reported here. Their structures were determined by means of chemical and spectroscopic methods. On the other hand, their inhibitory effects on sodium oleate-induced triglyceride (TG) overloading in HepG2 cells were evaluated. As a result, two new compounds ( and ), together with known isolates 7-11, 13, 14, 16-18, 20, 21, and 25 possessed significant inhibitory effects in the cells.
[Mh] Termos MeSH primário: Lepidium/química
Extratos Vegetais/química
Sementes/química
Triglicerídeos/metabolismo
[Mh] Termos MeSH secundário: Células Hep G2/efeitos de drogas
Humanos
Espectroscopia de Ressonância Magnética
Estrutura Molecular
Ácido Oleico/farmacologia
Extratos Vegetais/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Extracts); 0 (Triglycerides); 2UMI9U37CP (Oleic Acid); 399SL044HN (osteum)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170517
[Lr] Data última revisão:
170517
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170328
[St] Status:MEDLINE


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[PMID]:28174084
[Au] Autor:Zhang L; Zhao Q; Wang L; Zhao M; Zhao B
[Ad] Endereço:Division of Biorefinery Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
[Ti] Título:Protective effect of polysaccharide from maca (Lepidium meyenii) on Hep-G2 cells and alcoholic liver oxidative injury in mice.
[So] Source:Int J Biol Macromol;99:63-70, 2017 Jun.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:To study the characterization and hepatoprotective activity of polysaccharide from maca (Lepidium meyenii), the main polysaccharide from maca (MP-1) was obtained by DEAE-52 cellulose column. The average molecular weight of MP-1 was 1067.3kDa and the polysaccharide purity was 91.63%. In order to assess the antioxidant activities of MP-1, four kinds of methods were used, including scavenging hydroxyl radical, DPPH, superoxide anion radical, and FRAP, and the results indicated high antioxidant activities. Furthermore, hepatoprotective activity of MP-1 was studied both in vitro and vivo. In vitro, the alcohol induced Hep-G2 cells model was established to evaluate the protective effect of MP-1, which demonstrated MP-1 can alleviate alcohol damage in Hep-G2 cells. In vivo, the Institute of Cancer Researcch (ICR) mice were used to evaluate hepatoprotecive effects of MP-1 on alcoholic liver disease (ALD). Supplement with MP-1 supressed the triglyceride level both in serum and in hepatic tissue. In addition, MP-1 ameliorated serous transaminases increase induced by alcohol, including aspartate transaminase, alanine aminotransferase, and γ-glutamyl transpeptidase. Moreover, MP-1 also dramatically increased the superoxide dismutase, glutathione peroxidase, and glutathione s-transferase levels in alcoholic mice. Meantime, histopathologic results MP-1 lighten inflammation induced by alcohol. These results indicate that MP-1 possesses hepatoprotective activity against hepatic injury induced by alcohol.
[Mh] Termos MeSH primário: Doença Hepática Induzida por Substâncias e Drogas/metabolismo
Doença Hepática Induzida por Substâncias e Drogas/prevenção & controle
Etanol/toxicidade
Lepidium/química
Polissacarídeos/farmacologia
[Mh] Termos MeSH secundário: Animais
Antioxidantes/metabolismo
Doença Hepática Induzida por Substâncias e Drogas/enzimologia
Doença Hepática Induzida por Substâncias e Drogas/patologia
Citoproteção/efeitos de drogas
Células Hep G2
Humanos
Fígado/efeitos de drogas
Fígado/enzimologia
Fígado/metabolismo
Malondialdeído/metabolismo
Camundongos
Triglicerídeos/sangue
Triglicerídeos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Polysaccharides); 0 (Triglycerides); 3K9958V90M (Ethanol); 4Y8F71G49Q (Malondialdehyde)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170428
[Lr] Data última revisão:
170428
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170208
[St] Status:MEDLINE


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[PMID]:27719949
[Au] Autor:Zhang L; Li G; Wang S; Yao W; Zhu F
[Ad] Endereço:School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, Jiangsu Province, China.
[Ti] Título:Physicochemical properties of maca starch.
[So] Source:Food Chem;218:56-63, 2017 Mar 01.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Maca (Lepidium meyenii Walpers) is gaining research attention due to its unique bioactive properties. Starch is a major component of maca roots, thus representing a novel starch source. In this study, the properties of three maca starches (yellow, purple and black) were compared with commercially maize, cassava, and potato starches. The starch granule sizes ranged from 9.0 to 9.6µm, and the granules were irregularly oval. All the maca starches presented B-type X-ray diffraction patterns, with the relative degree of crystallinity ranging from 22.2 to 24.3%. The apparent amylose contents ranged from 21.0 to 21.3%. The onset gelatinization temperatures ranged from 47.1 to 47.5°C as indicated by differential scanning calorimetry. Significant differences were observed in the pasting properties and textural parameters among all of the studied starches. These characteristics suggest the utility of native maca starch in products subjected to low temperatures during food processing and other industrial applications.
[Mh] Termos MeSH primário: Lepidium/química
Amido/química
[Mh] Termos MeSH secundário: Amilose/análise
Varredura Diferencial de Calorimetria
Fenômenos Químicos
Manihot/química
Raízes de Plantas/química
Solanum tuberosum/química
Temperatura Ambiente
Difração de Raios X
Zea mays/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9005-25-8 (Starch); 9005-82-7 (Amylose)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170104
[Lr] Data última revisão:
170104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161010
[St] Status:MEDLINE


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[PMID]:27681650
[Au] Autor:Valdivia Cuya M; Yarasca De La Vega K; Lévano Sánchez G; Vásquez Cavero J; Temoche García H; Torres Torres L; Cruz Ornetta V
[Ad] Endereço:Animal Reproductive Physiology, Faculty of Biological Science, Universidad Nacional Mayor de San Marcos, Lima, Peru. mvaldiviac@unmsm.edu.pe.
[Ti] Título:Effect of Lepidium meyenii (maca) on testicular function of mice with chemically and physically induced subfertility.
[So] Source:Andrologia;48(8):927-34, 2016 Oct.
[Is] ISSN:1439-0272
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to evaluate the effect of Lepidium meyenii (maca) in chemically and physically subfertile mice. After 35 days, the following groups of mice were evaluated: control, sham, chemical subfertility, chemical subfertility-maca-supplemented, physical subfertility, physical subfertility-maca-supplemented and maca-supplemented only. Motility (32.36% ± 5.34%) and sperm count (44.4 ± 5.37 × 10(6) /ml) in the chemically and physically subfertile mice (11.81% ± 4.06%, 17.34 ± 13.07 × 10(6) /ml) decreased compared to the control (75.53% ± 2.97% and 57.4 ± 19.6 10(6) /ml) and sham (53.5% ± 7.86% and 58.4 ± 14.10 10(6) /ml). Maca was able to reverse the deleterious effect of motility (76.36 ± 1.97) as well as sperm count (53.5 ± 9.18 × 10(6) /ml) on chemical subfertility. In contrast, maca did not reverse the effects of induced physical subfertility nor motility (18.78% ± 14.41%) or sperm count (20.17 ± 11.20 × 10(6) /ml). The percentage of sperm DNA fragmentation in the physically subfertile mice increased (11.1% ± 19.29%) compared to the control (0.84% ± 0.85%). However, in the physically subfertile group, maca decreased sperm DNA fragmentation (2.29% ± 2.30%) closer to the sham (1.04% ± 0.62%) and the control (0.84% ± 0.85%). The group supplemented only with maca showed 0.54% ± 0.50% of spermatozoa with DNA fragmentation. Yet, the differences observed were statistically not significant. In conclusion, it appears that maca activates the cytochrome P450 system after chemically induced subfertility. However, it does not reverse the low mitochondrial membrane potential in spermatozoa compromised in the physical subfertility group.
[Mh] Termos MeSH primário: Fragmentação do DNA/efeitos de drogas
Infertilidade Masculina/fisiopatologia
Lepidium
Potencial da Membrana Mitocondrial/efeitos de drogas
Extratos Vegetais/farmacologia
Motilidade Espermática/efeitos de drogas
Espermatozoides/efeitos de drogas
Testículo/efeitos de drogas
[Mh] Termos MeSH secundário: Animais
Antifúngicos/toxicidade
Estudos de Casos e Controles
Radiação Eletromagnética
Infertilidade Masculina/induzido quimicamente
Infertilidade Masculina/etiologia
Cetoconazol/toxicidade
Masculino
Camundongos
Camundongos Endogâmicos BALB C
Contagem de Espermatozoides
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Plant Extracts); R9400W927I (Ketoconazole)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170124
[Lr] Data última revisão:
170124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160929
[St] Status:MEDLINE
[do] DOI:10.1111/and.12682


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[PMID]:27681649
[Au] Autor:Díaz P; Cardenas H; Orihuela PA
[Ad] Endereço:Laboratorio de Inmunología de la Reproducción, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago, Chile.
[Ti] Título:Red Maca (Lepidium meyenii) did not affect cell viability despite increased androgen receptor and prostate-specific antigen gene expression in the human prostate cancer cell line LNCaP.
[So] Source:Andrologia;48(8):922-6, 2016 Oct.
[Is] ISSN:1439-0272
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:We examined whether aqueous extract of Lepidium meyenii (red Maca) could inhibit growth, potentiate apoptotic activity of two anticancer drugs Taxol and 2-methoxyestradiol (2ME) or change mRNA expression for the androgen target genes, androgen receptor (Ar) and prostate-specific antigen (Psa) in the human prostate cancer cell line LNCaP. Red Maca aqueous extract at 0, 10, 20, 40 or 80 µg/ml was added to LNCaP cells, and viability was evaluated by the MTS assay at 24 or 48 hr after treatment. Furthermore, LNCaP cells were treated with 80 µg/ml of red Maca plus Taxol or 2ME 5 µM and viability was assessed 48 hr later. Finally, LNCaP cells were treated with red Maca 0, 20, 40 or 80 µg/ml, and 12 hr later, mRNA level for Ar or Psa was assessed by real-time PCR. Treatment with red Maca did not affect viability of LNCaP cells. Apoptotic activity induced by Taxol and 2ME in LNCaP cells was not altered with red Maca treatment. Relative expression of the mRNA for Ar and Psa increased with red Maca 20 and 40 µg/ml, but not at 80 µg/ml. We conclude that red Maca aqueous extract does not have toxic effects, but stimulates androgen signalling in LNCaP cells.
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
Sobrevivência Celular/efeitos de drogas
Expressão Gênica/efeitos de drogas
Calicreínas/efeitos de drogas
Lepidium
Extratos Vegetais/farmacologia
Antígeno Prostático Específico/efeitos de drogas
Neoplasias da Próstata/genética
RNA Mensageiro/efeitos de drogas
Receptores Androgênicos/efeitos de drogas
[Mh] Termos MeSH secundário: Apoptose/efeitos de drogas
Linhagem Celular Tumoral
Sinergismo Farmacológico
Estradiol/análogos & derivados
Estradiol/farmacologia
Humanos
Calicreínas/genética
Masculino
Paclitaxel/farmacologia
Antígeno Prostático Específico/genética
RNA Mensageiro/metabolismo
Reação em Cadeia da Polimerase em Tempo Real
Receptores Androgênicos/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Plant Extracts); 0 (RNA, Messenger); 0 (Receptors, Androgen); 4TI98Z838E (Estradiol); 6I2QW73SR5 (2-methoxyestradiol); EC 3.4.21.- (Kallikreins); EC 3.4.21.- (kallikrein-related peptidase 3, human); EC 3.4.21.77 (Prostate-Specific Antigen); P88XT4IS4D (Paclitaxel)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170124
[Lr] Data última revisão:
170124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160929
[St] Status:MEDLINE
[do] DOI:10.1111/and.12681


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[PMID]:27681648
[Au] Autor:Inoue N; Farfan C; Gonzales GF
[Ad] Endereço:Department of Biological and Physiological Sciences, Faculty of Sciences and Philosophy, Universidad Peruana Cayetano Heredia, Lima, Peru.
[Ti] Título:Effect of butanolic fraction of yellow and black maca (Lepidium meyenii) on the sperm count of adult mice.
[So] Source:Andrologia;48(8):915-21, 2016 Oct.
[Is] ISSN:1439-0272
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Lepidium meyenii, known as maca, is a popular nutraceutical food which is grown over 4,000 m above sea level in the Peruvian central highlands. Maca contains alkaloids, but there are no studies on their biological effects. The butanol fraction obtained from methanol extract of maca hypocotyls contains alkaloids. The effects of butanol/aqueous fractions partitioned from methanol extract of yellow and black maca were examined. Total phenolic content (TPC) and antioxidant capacity by 2,2'-diphenyl-1-picrylhydrazyl were used to evaluate maca fractions in vitro. Daily sperm production and sperm count in epididymis and vas deferens in mice were determined as biological effect of maca extracts in vivo. Yellow maca (21.7%±0.69) had better antioxidant capacity than black maca (18.2% ± 0.12; p < .01). Antioxidant activity was better in the methanolic fraction than in the aqueous fraction of yellow or black maca. TPC is higher in the aqueous fraction than in the methanolic extract of yellow or black maca. Black maca administration resulted in higher concentration of sperm count in epididymis and vas deferens compared to yellow maca. A higher biological effect was observed in methanolic extract and in aqueous extract than in the butanol fraction of maca. In conclusion, better biological effect was observed in the methanolic extract of maca than in its partitioned fractions.
[Mh] Termos MeSH primário: Antioxidantes/farmacologia
Lepidium
Extratos Vegetais/farmacologia
Espermatozoides/efeitos de drogas
[Mh] Termos MeSH secundário: Animais
Epididimo
Masculino
Camundongos
Contagem de Espermatozoides
Ducto Deferente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Plant Extracts)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170124
[Lr] Data última revisão:
170124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160929
[St] Status:MEDLINE
[do] DOI:10.1111/and.12679



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