Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.156.100.666.222 [Categoria DeCS]
Referências encontradas : 231 [refinar]
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  1 / 231 MEDLINE  
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[PMID]:29261256
[Au] Autor:Özel HU
[Ti] Título:The effects of dusts of bartin cement factory on Taurus Cedar (Cedru libani A Rich.) seeds' germination.
[So] Source:J Environ Biol;37(6):1331-34, 2016 11.
[Is] ISSN:0254-8704
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:The present study effects of pollutant particles from Bartin Cement Factory on development of seeds and leaves of Taurus Cedar (Cedrus libani Rich.) which in plantation area around the factory were examined. For this purpose, seeds were collected from sampling trees located 200m, 400m, 600m, 800m and 1000m (control) and germination percentage of seeds, and total chlorophyll content of seedlings were determined. Moreover, in the present study, the air movement around Bartin Cement Factory and level of pollutants in sampling areas were also determined. The directions of the prevailing winds around the cement factory were North, and Northwest. From the aspect of pollutant material level, the array of sampling areas was SP1>SP2>SP3>SP4>C. On the other hand, evaluating the results of mean seed germination percentages, a significant difference (P<0.01) was noted between control and seeds of Taurus Cedar at various distances from are pollutant source. From the aspect of the power of seeds collected with 3 repetitions from Taurus Cedars located at various distances, it a significant difference (P<0.01) was found between the control and experimental groups. Accordingly, from both the aspects of mean germination percentage and seed power, an apparent increase was observed from 400 m distance. On the other hand, as the distance from cement factory increased, total chlorophyll content in Taurus Cedar seedlings increases significantly. Results of bilateral regression analysis evaluated exponential relationship at level 97% between these 2 variables.
[Mh] Termos MeSH primário: Cedrus/efeitos dos fármacos
Materiais de Construção
Poeira
Poluentes Ambientais/toxicidade
Germinação/efeitos dos fármacos
Sementes/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Dust); 0 (Environmental Pollutants)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180219
[Lr] Data última revisão:
180219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171221
[St] Status:MEDLINE


  2 / 231 MEDLINE  
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[PMID]:28095708
[Au] Autor:Sharma S; Bhatt V; Kumar N; Singh B; Sharma U
[Ad] Endereço:a Natural Product Chemistry and Process Development Department , CSIR-Institute of Himalayan Bioresource Technology , Palampur , India.
[Ti] Título:Locational comparison of essential oils from selected conifers of Himachal Pradesh.
[So] Source:Nat Prod Res;31(13):1578-1582, 2017 Jul.
[Is] ISSN:1478-6427
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Nine samples of essential oil from needles of three conifers of Pinacea family namely Abies pindrow, Picea smithiana and Cedrus deodara collected from three different locations of Himachal Pradesh (India) were evaluated using gas chromatography and gas chromatography-mass spectrometry. A total of 31, 17 and 13 compounds were identified from essential oil of A. pindrow, P. smithiana and C. deodara, respectively. Among the characterised components, monoterpenoid hydrocarbons were predominated. α-Pinene, ß-pinene, ß-merycene, limonene and camphene were characterised as major components. Oil of C. deodara has significant effect of location on its oil composition. Principle component analysis on gas chromatographic data reveals variation in chemical composition which may be attributed to altitude and environmental conditions.
[Mh] Termos MeSH primário: Coniferophyta/química
Meio Ambiente
Óleos Voláteis/química
[Mh] Termos MeSH secundário: Abies/química
Compostos Bicíclicos com Pontes/análise
Compostos Bicíclicos com Pontes/isolamento & purificação
Cedrus/química
Cromatografia Gasosa
Cicloexenos/análise
Cicloexenos/isolamento & purificação
Cromatografia Gasosa-Espectrometria de Massas
Índia
Monoterpenos/análise
Monoterpenos/química
Monoterpenos/isolamento & purificação
Terpenos/análise
Terpenos/isolamento & purificação
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bridged Bicyclo Compounds); 0 (Cyclohexenes); 0 (Monoterpenes); 0 (Oils, Volatile); 0 (Terpenes); 4MS8VHZ1HJ (beta-pinene); 9MC3I34447 (limonene); G3VG94Z26E (camphene); JPF3YI7O34 (alpha-pinene)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170119
[St] Status:MEDLINE
[do] DOI:10.1080/14786419.2016.1277354


  3 / 231 MEDLINE  
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[PMID]:27916264
[Au] Autor:Al-Alam J; Fajloun Z; Chbani A; Millet M
[Ad] Endereço:Azm Center for Research in Biotechnology and Its Applications, Doctoral School of Science and Technology, Lebanese University, El Mittein Street, Tripoli, Lebanon; Institute of Chemistry and Processes for Energy, Environment and Health, ICPEES UMR 7515, Group of Physical Chemistry of the Atmosphere,
[Ti] Título:The use of conifer needles as biomonitor candidates for the study of temporal air pollution variation in the Strasbourg region.
[So] Source:Chemosphere;168:1411-1421, 2017 Feb.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The continuous emission of polluting chemicals into the atmosphere requires the implementation of monitoring of ambient air quality. The use of vegetation for environmental monitoring can be considered as a simple monitoring technique by providing a cheap and accessible matrix. In this study, needles of two conifers (Pinus nigra and Cedrus atlantica), were used for the consecutive biomonitoring of multipollutants such as pesticides, polychlorinated biphenyls, and polyaromatic hydrocarbons (PAHs) in an urban area in Strasbourg (France). The extraction was performed by accelerated solvent extraction, solid-phase extraction, and solid-phase microextraction and was followed by gas chromatography coupled to tandem mass spectrometry and liquid chromatography coupled to tandem mass spectrometry analyses. The results obtained for conifer samples collected in 5 successive weeks (April 09-May 07, 2015) show a similar variation of the different types of pollutants. A pollution peak was observed during the second week of analysis, and the concentration of all pollutants then decreased to complete disappearance at the end of the sampling period. PAHs were the most concentrated with a total concentration of about 35.87 ng g , and naphthalene was, among these pollutants, the most concentrated with a total concentration of about 15.1 ng g . The analysis of meteorological data during this period suggests that the results correlated with climatic conditions that widely vary during this period of the year. The results show that the concentration peak was obtained when no precipitation was detected.
[Mh] Termos MeSH primário: Poluentes Atmosféricos/análise
Cedrus
Pinus
Folhas de Planta/química
[Mh] Termos MeSH secundário: Poluição do Ar/análise
Cidades
Monitoramento Ambiental/métodos
França
Praguicidas/análise
Bifenilos Policlorados/análise
Hidrocarbonetos Aromáticos Policíclicos/análise
Extração em Fase Sólida
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Air Pollutants); 0 (Pesticides); 0 (Polycyclic Aromatic Hydrocarbons); DFC2HB4I0K (Polychlorinated Biphenyls)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170127
[Lr] Data última revisão:
170127
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161206
[St] Status:MEDLINE


  4 / 231 MEDLINE  
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[PMID]:27844339
[Au] Autor:Yamada R; Nakashima K; Asai-Nakashima N; Tokuhara W; Ishida N; Katahira S; Kamiya N; Ogino C; Kondo A
[Ad] Endereço:Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo, 657-8501, Japan.
[Ti] Título:Direct Ethanol Production from Ionic Liquid-Pretreated Lignocellulosic Biomass by Cellulase-Displaying Yeasts.
[So] Source:Appl Biochem Biotechnol;182(1):229-237, 2017 May.
[Is] ISSN:1559-0291
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Among the many types of lignocellulosic biomass pretreatment methods, the use of ionic liquids (ILs) is regarded as one of the most promising strategies. In this study, the effects of four kinds of ILs for pretreatment of lignocellulosic biomass such as bagasse, eucalyptus, and cedar were evaluated. In direct ethanol fermentation from biomass incorporated with ILs by cellulase-displaying yeast, 1-butyl-3-methylimidazolium acetate ([Bmim][OAc]) was the most effective IL. The ethanol production and yield from [Bmim][OAc]-pretreated bagasse reached 0.81 g/L and 73.4% of the theoretical yield after fermentation for 96 h. The results prove the initial concept, in which the direct fermentation from lignocellulosic biomass effectively promoted by the pretreatment with IL.
[Mh] Termos MeSH primário: Biocombustíveis
Etanol/metabolismo
Glucose/biossíntese
Imidazóis/química
Líquidos Iônicos/química
Lignina/metabolismo
Xilose/biossíntese
[Mh] Termos MeSH secundário: Aspergillus/efeitos dos fármacos
Aspergillus/enzimologia
Biomassa
Cedrus/química
Celulases/metabolismo
Celulose/química
Eucalyptus/química
Fermentação
Proteínas Fúngicas/metabolismo
Imidazóis/farmacologia
Líquidos Iônicos/farmacologia
Cinética
Saccharomyces cerevisiae/efeitos dos fármacos
Saccharomyces cerevisiae/enzimologia
Trichoderma/efeitos dos fármacos
Trichoderma/enzimologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (1-butyl-3-methylimidazolium); 0 (Biofuels); 0 (Fungal Proteins); 0 (Imidazoles); 0 (Ionic Liquids); 11132-73-3 (lignocellulose); 3K9958V90M (Ethanol); 9004-34-6 (Cellulose); 9005-53-2 (Lignin); 9006-97-7 (bagasse); A1TA934AKO (Xylose); EC 3.2.1.- (Cellulases); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170619
[Lr] Data última revisão:
170619
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161116
[St] Status:MEDLINE
[do] DOI:10.1007/s12010-016-2322-2


  5 / 231 MEDLINE  
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[PMID]:27719936
[Au] Autor:Wu Y; Bai J; Zhong K; Huang Y; Gao H
[Ad] Endereço:Department of Food Science and Technology, College of Light Industry, Textile and Food Engineering, Sichuan University, Chengdu 610065, China.
[Ti] Título:A dual antibacterial mechanism involved in membrane disruption and DNA binding of 2R,3R-dihydromyricetin from pine needles of Cedrus deodara against Staphylococcus aureus.
[So] Source:Food Chem;218:463-470, 2017 Mar 01.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The antibacterial activity and mechanism of 2R,3R-dihydromyricetin (DMY) against Staphylococcus aureus were investigated. The minimum inhibitory concentration of DMY against S. aureus was 0.125mg/ml, and the growth inhibitory assay also revealed that DMY showed a potent antibacterial activity against S. aureus. Massive nucleotide leakage and flow cytometric analysis demonstrated that DMY disrupted the membrane integrity of S. aureus. Morphological changes and membrane hyperpolarization of S. aureus cells treated with DMY further suggested that DMY destroyed cell membrane. Meanwhile, DMY probably interacted with membrane lipids and proteins, causing a significant reduction in membrane fluidity and changes in conformation of membrane protein. Moreover, DMY could interact with S. aureus DNA through the groove binding mode. Overall, the results suggested that DMY could be applied as a candidate for the development of new food preservatives as it achieved bactericidal activity by damaging cell membrane and binding to intracellular DNA.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Cedrus/química
DNA/metabolismo
Flavonóis/farmacologia
Staphylococcus aureus/efeitos dos fármacos
[Mh] Termos MeSH secundário: Membrana Celular/efeitos dos fármacos
Fluidez de Membrana/efeitos dos fármacos
Testes de Sensibilidade Microbiana
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Flavonols); 9007-49-2 (DNA); KD8QND6427 (dihydromyricetin)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161011
[St] Status:MEDLINE


  6 / 231 MEDLINE  
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[PMID]:27511616
[Au] Autor:Sun Q; Liu Y; Yuan H; Lian B
[Ad] Endereço:Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Microbiology, College of Life Sciences, Nanjing Normal University, Nanjing, Jiangsu, China.
[Ti] Título:The effect of environmental contamination on the community structure and fructification of ectomycorrhizal fungi.
[So] Source:Microbiologyopen;6(1), 2017 Feb.
[Is] ISSN:2045-8827
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Ectomycorrhizal fungi are an essential component of forest ecosystems, most of which can form edible and medical fruiting bodies. Although many studies have focused on the fructification of ectomycorrhizal fungi in phenology, the impact of environmental contamination, especially living garbage, on the formation of fruiting body is still unknown. A field investigation, combined with a high-throughput sequencing method, was used to study the effect of living garbage pollution on the fructification and hypogeous community structure of ectomycorrhizal fungi symbiosing with cedar (Cedrus deodara (Roxb.) G. Don). The results showed that garbage significantly altered soil abiotic and biotic properties, increasing soil urease activity, decreasing the soil exchangeable metal content and phosphatase activity, and ultimately inhibiting the formation of fruiting bodies. The pollution of garbage also changed the community structure of hypogeous ectomycorrhizal fungi where ectomycorrhizal ascomycetes dominated. In unpolluted sites, the relative abundance of ectomycorrhizal ascomycetes and basidiomycetes were almost equal. Although no fruiting bodies were observed in that soil polluted by living garbage, the sequencing result showed that various ectomycorrhizal fungi were present underground, suggesting that these taxonomic fungi had the potential to cope with adverse conditions. This study not only provided a deeper understanding of the relationship between ectomycorrhizal fungal communities and prevailing environmental conditions, but provided a new pathway for the excavation and utilization of the resource of antistress ectomycorrhizal fungi.
[Mh] Termos MeSH primário: Ascomicetos/crescimento & desenvolvimento
Basidiomycota/crescimento & desenvolvimento
Cedrus/microbiologia
Poluentes Ambientais/metabolismo
Carpóforos/crescimento & desenvolvimento
Micorrizas/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Ascomicetos/metabolismo
Sequência de Bases
Basidiomycota/metabolismo
DNA Fúngico/genética
Poluição Ambiental
Florestas
Carpóforos/metabolismo
Análise de Sequência de DNA
Solo/química
Microbiologia do Solo
Urease/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Fungal); 0 (Environmental Pollutants); 0 (Soil); EC 3.5.1.5 (Urease)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160812
[St] Status:MEDLINE
[do] DOI:10.1002/mbo3.396


  7 / 231 MEDLINE  
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[PMID]:28049962
[Au] Autor:Awaya A
[Ad] Endereço:Dermatology & Epidemiology Research Institute (DERI).
[Ti] Título:Suppressive influence of seasonal influenza epidemic on Kawasaki disease onset.
[So] Source:Nihon Rinsho Meneki Gakkai Kaishi;39(6):528-537, 2016.
[Is] ISSN:1349-7413
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:  Kawasaki disease (KD) is an acute systemic vasculitis presenting as an infantile febrile disease. In Japan, the widespread cedar plantation commenced in 1945 has been correlated with the increased incidences of both KD and allergic rhinitis (pollinosis) since the early 1960s. We previously showed that KD was a pollen-induced, delayed-type hypersensitivity that displays biphasic peaks in both summer and winter. KD incidences decrease suddenly around February, particularly after influenza epidemics. Here we investigated the reason for a drastic decrease in KD onsets directly before spring pollen release following rapid increase after autumn pollen release leading to the biphasic pattern. We separately analyzed weekly incidences of KD and influenza in Tokyo (1987-2010) and Kanagawa (1991-2002). Repeated measures for the analysis of variance followed by Bonferroni's multiple comparison tests were performed to compare KD incidence over 3 consecutive weeks, including the weeks when the mean KD prevalence showed the steepest decrease. Next, the week with peak influenza incidence was reset for each year. KD incidence over 3 consecutive weeks, including the new origin week (adjusted week 0), was similarly analyzed. In Tokyo and Kanagawa, KD incidence significantly decreased only after resetting the influenza peak time. These findings suggested that influenza epidemics suppressed KD onset.
[Mh] Termos MeSH primário: Epidemias
Influenza Humana/epidemiologia
Síndrome de Linfonodos Mucocutâneos/epidemiologia
Síndrome de Linfonodos Mucocutâneos/imunologia
[Mh] Termos MeSH secundário: Cedrus/efeitos adversos
Cedrus/imunologia
Criança
Seres Humanos
Incidência
Vacinas contra Influenza/administração & dosagem
Interferon beta/administração & dosagem
Japão/epidemiologia
Síndrome de Linfonodos Mucocutâneos/tratamento farmacológico
Síndrome de Linfonodos Mucocutâneos/prevenção & controle
Pólen/efeitos adversos
Pólen/imunologia
Estações do Ano
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Influenza Vaccines); 77238-31-4 (Interferon-beta)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170316
[Lr] Data última revisão:
170316
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170105
[St] Status:MEDLINE
[do] DOI:10.2177/jsci.39.528


  8 / 231 MEDLINE  
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[PMID]:27935944
[Au] Autor:Goring SJ; Mladenoff DJ; Cogbill CV; Record S; Paciorek CJ; Jackson ST; Dietze MC; Dawson A; Matthes JH; McLachlan JS; Williams JW
[Ad] Endereço:Department of Geography, University of Wisconsin-Madison, Madison, Wisconsin, United States.
[Ti] Título:Novel and Lost Forests in the Upper Midwestern United States, from New Estimates of Settlement-Era Composition, Stem Density, and Biomass.
[So] Source:PLoS One;11(12):e0151935, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: EuroAmerican land-use and its legacies have transformed forest structure and composition across the United States (US). More accurate reconstructions of historical states are critical to understanding the processes governing past, current, and future forest dynamics. Here we present new gridded (8x8km) reconstructions of pre-settlement (1800s) forest composition and structure from the upper Midwestern US (Minnesota, Wisconsin, and most of Michigan), using 19th Century Public Land Survey System (PLSS), with estimates of relative composition, above-ground biomass, stem density, and basal area for 28 tree types. This mapping is more robust than past efforts, using spatially varying correction factors to accommodate sampling design, azimuthal censoring, and biases in tree selection. CHANGES IN FOREST STRUCTURE: We compare pre-settlement to modern forests using US Forest Service Forest Inventory and Analysis (FIA) data to show the prevalence of lost forests (pre-settlement forests with no current analog), and novel forests (modern forests with no past analogs). Differences between pre-settlement and modern forests are spatially structured owing to differences in land-use impacts and accompanying ecological responses. Modern forests are more homogeneous, and ecotonal gradients are more diffuse today than in the past. Novel forest assemblages represent 28% of all FIA cells, and 28% of pre-settlement forests no longer exist in a modern context. Lost forests include tamarack forests in northeastern Minnesota, hemlock and cedar dominated forests in north-central Wisconsin and along the Upper Peninsula of Michigan, and elm, oak, basswood and ironwood forests along the forest-prairie boundary in south central Minnesota and eastern Wisconsin. Novel FIA forest assemblages are distributed evenly across the region, but novelty shows a strong relationship to spatial distance from remnant forests in the upper Midwest, with novelty predicted at between 20 to 60km from remnants, depending on historical forest type. The spatial relationships between remnant and novel forests, shifts in ecotone structure and the loss of historic forest types point to significant challenges for land managers if landscape restoration is a priority. The spatial signals of novelty and ecological change also point to potential challenges in using modern spatial distributions of species and communities and their relationship to underlying geophysical and climatic attributes in understanding potential responses to changing climate. The signal of human settlement on modern forests is broad, spatially varying and acts to homogenize modern forests relative to their historic counterparts, with significant implications for future management.
[Mh] Termos MeSH primário: Conservação dos Recursos Naturais
Agricultura Florestal/tendências
Dispersão Vegetal/fisiologia
Árvores/fisiologia
[Mh] Termos MeSH secundário: Biomassa
Cedrus/fisiologia
Ecossistema
Florestas
Cicutas (Apiáceas)/fisiologia
Seres Humanos
Larix/fisiologia
Meio-Oeste dos Estados Unidos
Filogeografia
Caules de Planta/fisiologia
Quercus/fisiologia
Tilia/fisiologia
Ulmus/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170705
[Lr] Data última revisão:
170705
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161210
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0151935


  9 / 231 MEDLINE  
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[PMID]:27548123
[Au] Autor:Wu Y; Bai J; Zhong K; Huang Y; Qi H; Jiang Y; Gao H
[Ad] Endereço:Department of Food Science and Technology, College of Light Industry, Textile and Food Engineering, Sichuan University, Chengdu 610065, China. wyp9202@163.com.
[Ti] Título:Antibacterial Activity and Membrane-Disruptive Mechanism of 3-p-trans-Coumaroyl-2-hydroxyquinic Acid, a Novel Phenolic Compound from Pine Needles of Cedrus deodara, against Staphylococcus aureus.
[So] Source:Molecules;21(8), 2016 Aug 18.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Recently, we reported that a novel phenolic compound isolated from Cedrus deodara, 3-p-trans-coumaroyl-2-hydroxyquinic acid (CHQA), exhibits a potent antioxidant activity. The present study aimed to evaluate the antibacterial activity of CHQA against eleven food-borne pathogens and to elucidate its mechanism of action against Staphylococcus aureus. The results from minimum inhibitory concentration (MIC) determinations showed that CHQA exhibited moderate inhibitory effects on all of the tested pathogens with MIC values ranging from 2.5-10 mg/mL. Membrane potential measurements and flow cytometric analysis demonstrated that CHQA damaged the cytoplasmic membrane of S. aureus, causing a significant membrane hyperpolarization with a loss of membrane integrity. Moreover, CHQA induced an increase in membrane fluidity and conformational changes in membrane protein of S. aureus, suggesting that CHQA probably acts on the cell membrane by interactions with membrane lipid and protein. Transmission electron microscopic observations further confirmed that CHQA disrupted the cell membrane of S. aureus and caused severe morphological changes, which even led to leakage of intracellular constituents. These findings indicated that CHQA could have the potential to serve as a natural antibacterial agent to control and prevent the growth of pathogens in food and in food-processing environments.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Cedrus/química
Fenóis/farmacologia
Staphylococcus aureus/efeitos dos fármacos
[Mh] Termos MeSH secundário: Antibacterianos/química
Membrana Celular/efeitos dos fármacos
Potenciais da Membrana
Testes de Sensibilidade Microbiana
Estrutura Molecular
Fenóis/química
Extratos Vegetais/química
Extratos Vegetais/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Phenols); 0 (Plant Extracts)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170414
[Lr] Data última revisão:
170414
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160823
[St] Status:MEDLINE


  10 / 231 MEDLINE  
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[PMID]:27461104
[Au] Autor:Shi X; Liu D; Zhang J; Hu P; Shen W; Fan B; Ma Q; Wang X
[Ad] Endereço:Gansu Provincial Academy of Medical Sciences, Lanzhou, 730050, China. shixiaofeng2005@sina.com.
[Ti] Título:Extraction and purification of total flavonoids from pine needles of Cedrus deodara contribute to anti-tumor in vitro.
[So] Source:BMC Complement Altern Med;16:245, 2016 Jul 26.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Cedrus deodara is one of the traditional Chinese medicinal herbs that exhibits a line of biological activities. The current study extracted the total flavonoids from the pine needles of Cedrus deodara (TFPNCD), and investigated its anti-cancer effects in tumor cell lines. METHODS: The total flavonoids was extracted from pine needles of Cedrus deodara by ethanol hot refluxing and purified by HPD722 macroporous resin. The contents of total flavonoids and the active ingredients of TFPNCD were analyzed through UV and HPLC. MTT assay was used to investigate its inhibitory effect on tumor cell lines. The flow cytometry was employed to determine the apoptosis and cell cycle distribution after treated TFPNCD on HepG2 cells. RESULTS: The TFPNCD, in which the contents of total flavonoid reached up to 54.28 %, and the major ingredients of myricetin, quercetin, kaempferol and isorhamnetin in TFPNCD were 1.89, 2.01, 2.94 and 1.22 mg/g, respectively. The MTT assays demonstrated that TFPNCD inhibited the growth of HepG2 cells in a dose-dependent manner, with the IC50 values of 114.12 µg/mL. By comparison, TFPNCD inhibited the proliferation to a less extent in human cervical carcinoma HeLa, gastric cancer MKN28 cells, glioma SHG-44 cells and lung carcinoma A549 than HepG2 cells. We found that even at the lower doses, the total flavonoids effectively inhibited the proliferation of HepG2 cells. Comparison of IC50 values implicated that HepG2 cells might be more sensitive to the treatment with total flavonoids. TFPNCD was able to increase the population of HepG2 cells in G0 /G1 phase. Meanwhile, TFPNCD treatment increased the percentage of apoptotic HepG2 cells. CONCLUSION: These data suggested that TFPNCD might have therapeutic potential in cancer through the regulation of cell cycle and apoptosis.
[Mh] Termos MeSH primário: Antineoplásicos
Cedrus/química
Flavonoides
Folhas de Planta/química
[Mh] Termos MeSH secundário: Antineoplásicos/química
Antineoplásicos/isolamento & purificação
Antineoplásicos/farmacologia
Apoptose/efeitos dos fármacos
Ciclo Celular/efeitos dos fármacos
Linhagem Celular Tumoral
Proliferação Celular/efeitos dos fármacos
Flavonoides/química
Flavonoides/isolamento & purificação
Flavonoides/farmacologia
Células Hep G2
Seres Humanos
Extratos Vegetais/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Flavonoids); 0 (Plant Extracts)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160728
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-016-1249-z



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