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Pesquisa : B01.650.940.800.575.156.199 [Categoria DeCS]
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[PMID]:28610390
[Au] Autor:Skelley P; Xu G; Tang W; Lindström AJ; Marler T; Khuraijam JS; Singh R; Radha P; Rich S
[Ad] Endereço:Florida State Collection of Arthropods, Florida Department of Agriculture - DPI, P.O.Box 147100, Gainesville, FL 32614-7100, USA.. Paul.Skelley@FreshFromFlorida.com.
[Ti] Título:Review of Cycadophila Xu, Tang & Skelley (Coleoptera: Erotylidae: Pharaxonothinae) inhabiting Cycas (Cycadaceae) in Asia, with descriptions of a new subgenus and thirteen new species.
[So] Source:Zootaxa;4267(1):1-63, 2017 05 12.
[Is] ISSN:1175-5334
[Cp] País de publicação:New Zealand
[La] Idioma:eng
[Ab] Resumo:The genus Cycadophila Xu, Tang & Skelley (Coleoptera: Erotylidae: Pharaxonothinae) associated with Cycas L. (Cycadacaeae) in Asia is reviewed. Strobilophila, new subgenus, with five species is described: Cycadophila (Strobilophila) assamensis new species, C. (S.) hiepi new species, C. (S.) kwaiensis new species, C. (S.) tansachai new species and C. (S.) yangi new species, all associated with Cycas. For the nominate subgenus Cycadophila eight new species are described, Cycadophila (Cycadophila) abyssa new species, C. (C.) collina new species,C. (C.) samara new species, C. (C.) convexa new species, C. (C.) cyclochasma new species, C. (C.) eurynota new species, C. (C.) papua new species, and C. (C.) torquata new species and four new generic combinations are proposed: C. (C.) vittata (Arrow) new combination, C. (C.) discimaculata (Mader) new combination, C. (C.) intermedia (Chûjô) new combination, and C. (C.) lata (Grouvelle) new combination. Only the first three listed species of the nominate subgenus have known associations with Cycas. Species are distinguished on the basis of morphology and/or by analysis of 16S rRNA gene sequences. The larva of subgenus Strobilophila is described based on individuals collected together with adults and matched with analysis of 16S rRNA gene sequences. Keys to subgenera and species of known adults and larvae are provided.
[Mh] Termos MeSH primário: Coleópteros
[Mh] Termos MeSH secundário: Animais
Ásia
Cycas
Larva
RNA Ribossômico 16S
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170615
[St] Status:MEDLINE
[do] DOI:10.11646/zootaxa.4267.1.1


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[PMID]:28598725
[Au] Autor:Chernoff N; Hill DJ; Diggs DL; Faison BD; Francis BM; Lang JR; Larue MM; Le TT; Loftin KA; Lugo JN; Schmid JE; Winnik WM
[Ad] Endereço:a U.S. Environmental Protection Agency, Office of Research and Development , National Health and Environmental Effects Research Laboratory , Research Triangle Park , NC , USA.
[Ti] Título:A critical review of the postulated role of the non-essential amino acid, ß-N-methylamino-L-alanine, in neurodegenerative disease in humans.
[So] Source:J Toxicol Environ Health B Crit Rev;20(4):1-47, 2017.
[Is] ISSN:1521-6950
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The compound BMAA (ß-N-methylamino-L-alanine) has been postulated to play a significant role in four serious neurological human diseases: Amyotrophic Lateral Sclerosis/Parkinsonism Dementia Complex (ALS/PDC) found on Guam, and ALS, Parkinsonism, and dementia that occur globally. ALS/PDC with symptoms of all three diseases first came to the attention of the scientific community during and after World War II. It was initially associated with cycad flour used for food because BMAA is a product of symbiotic cycad root-dwelling cyanobacteria. Human consumption of flying foxes that fed on cycad seeds was later suggested as a source of BMAA on Guam and a cause of ALS/PDC. Subsequently, the hypothesis was expanded to include a causative role for BMAA in other neurodegenerative diseases including Alzheimer's disease (AD) through exposures attributed to proximity to freshwaters and/or consumption of seafood due to its purported production by most species of cyanobacteria. The hypothesis that BMAA is the critical factor in the genesis of these neurodegenerative diseases received considerable attention in the medical, scientific, and public arenas. This review examines the history of ALS/PDC and the BMAA-human disease hypotheses; similarities and differences between ALS/PDC and the other diseases with similar symptomologies; the relationship of ALS/PDC to other similar diseases, studies of BMAA-mediated effects in lab animals, inconsistencies and data gaps in the hypothesis; and other compounds and agents that were suggested as the cause of ALS/PDC on Guam. The review concludes that the hypothesis of a causal BMAA neurodegenerative disease relationship is not supported by existing data.
[Mh] Termos MeSH primário: Diamino Aminoácidos/toxicidade
Cianobactérias/metabolismo
Doenças Neurodegenerativas/etiologia
[Mh] Termos MeSH secundário: Doença de Alzheimer/etiologia
Doença de Alzheimer/fisiopatologia
Esclerose Amiotrófica Lateral/etiologia
Esclerose Amiotrófica Lateral/fisiopatologia
Animais
Cycas/toxicidade
Farinha/toxicidade
Seres Humanos
Doenças Neurodegenerativas/fisiopatologia
Neurotoxinas/toxicidade
Transtornos Parkinsonianos/etiologia
Transtornos Parkinsonianos/fisiopatologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Amino Acids, Diamino); 0 (Neurotoxins); 108SA6URTV (beta-N-methylamino-L-alanine)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170629
[Lr] Data última revisão:
170629
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170610
[St] Status:MEDLINE
[do] DOI:10.1080/10937404.2017.1297592


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[PMID]:28291394
[Au] Autor:Clarke C; Burney D
[Ad] Endereço:From the Veterinary Emergency & Referral Group South, Brooklyn, New York (C.C.); Veterinary Specialists of North Texas, Dallas, Texas (D.B.); and Gulf Coast Veterinary Specialists, Houston, Texas (D.B.).
[Ti] Título:Cycad Palm Toxicosis in 14 Dogs from Texas.
[So] Source:J Am Anim Hosp Assoc;53(3):159-166, 2017 May/Jun.
[Is] ISSN:0587-2871
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The goal of this study is to report clinical information, diagnostic findings, and treatment modalities; assess variables that may help distinguish survivors from nonsurvivors; and review the outcome of cycad palm toxicosis in dogs. Fourteen client-owned dogs with confirmed cycad palm ingestion were identified by reviewing the medical record database at Gulf Coast Veterinary Specialists. Information on signalment, time of ingestion to presentation, clinical signs, physical examination findings, initial and peak/nadir laboratory abnormalities, radiographic and ultrasonographic findings, treatment modalities, liver histopathology, and clinical outcome was retrieved. Of the 14 dogs, nine (64%) died as a direct result of cycad palm intoxication, and three survivors had persistently elevated liver enzymes, signifying residual liver damage. Despite decontamination, patients continued to display evidence of illness, indicating rapid absorption of toxins. When evaluating initial and peak/nadir laboratory values, nadir serum albumin levels and nadir platelet counts were significantly lower in nonsurvivors compared to survivors (1.25 g/dL [0.4-2.1 g/dL] versus 2.6 g/dL [1.7-3.4 g/dL] and 21 × 10 [0-64 × 10 ] versus 62 × 10 [6-144 × 10 ], respectively). In this cohort of dogs, the case fatality rate was higher than previously reported. Nadir serum albumin levels and nadir platelet counts may help distinguish potential survivors from nonsurvivors.
[Mh] Termos MeSH primário: Cycas/toxicidade
Doenças do Cão/induzido quimicamente
[Mh] Termos MeSH secundário: Animais
Antibacterianos/uso terapêutico
Transfusão de Sangue/veterinária
Cardiotônicos/uso terapêutico
Doenças do Cão/tratamento farmacológico
Doenças do Cão/epidemiologia
Doenças do Cão/mortalidade
Cães
Dopamina/uso terapêutico
Feminino
Fármacos Gastrointestinais/uso terapêutico
Soluções Isotônicas/administração & dosagem
Masculino
Plantas Tóxicas
Estudos Retrospectivos
Análise de Sobrevida
Texas/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Cardiotonic Agents); 0 (Gastrointestinal Agents); 0 (Isotonic Solutions); 0 (crystalloid solutions); VTD58H1Z2X (Dopamine)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170629
[Lr] Data última revisão:
170629
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170315
[St] Status:MEDLINE
[do] DOI:10.5326/JAAHA-MS-6517


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[PMID]:27391307
[Au] Autor:Marler TE; Lindström AJ; Marler PN
[Ad] Endereço:a Western Pacific Tropical Research Center, University of Guam, UOG Station , Mangilao , Guam , USA.
[Ti] Título:Chilades pandava mothers discriminate among Cycas species during oviposition choice tests, but only in an endemic naïve population.
[So] Source:Plant Signal Behav;11(8):e1208879, 2016 Aug 02.
[Is] ISSN:1559-2324
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Host and non-host plant species communicate with insect herbivores to influence oviposition decisions. We studied if Chilades pandava female adults expressed oviposition preferences among host Cycas species in 2 choice tests, counting 39,420 eggs among assays from 4 butterfly populations. A naïve butterfly population from Cycas nongnoochiae habitat oviposited 2.2-fold more eggs on leaves of Cycas species that are susceptible to butterfly herbivory than on leaves of its native host Cycas nongnoochiae. In contrast, Chilades pandava populations experienced with novel Cycas species in Thailand, Philippines, and Guam exhibited no preference in choice tests between leaves of susceptible versus leaves of minimally damaged Cycas species. The results indicated that oviposition deterrents and/or stimulants partly mediate the sustainable relationship between an endemic Cycas species and the naïve Chilades pandava population from its habitat. Alternatively, differences in infochemicals among Cycas species do not enable discrimination in oviposition choices for Chilades pandava populations that have experienced Cycas species exhibiting no evolutionary history with Chilades pandava.
[Mh] Termos MeSH primário: Cycas/parasitologia
Herbivoria/fisiologia
Oviposição/fisiologia
[Mh] Termos MeSH secundário: Animais
Comportamento Animal/fisiologia
Borboletas/fisiologia
Ecossistema
Feminino
Mães
Folhas de Planta/parasitologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171020
[Lr] Data última revisão:
171020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160709
[St] Status:MEDLINE
[do] DOI:10.1080/15592324.2016.1208879


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[PMID]:27124298
[Au] Autor:Hu Y; Deng T; Chen L; Wu H; Zhang S
[Ad] Endereço:State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, Guangdong, China.
[Ti] Título:Selection and Validation of Reference Genes for qRT-PCR in Cycas elongata.
[So] Source:PLoS One;11(4):e0154384, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Quantitative reverse transcription PCR (qRT-PCR) is a sensitive technique used in gene expression studies. To achieve a reliable quantification of transcripts, appropriate reference genes are required for comparison of transcripts in different samples. However, few reference genes are available for non-model taxa, and to date, reliable reference genes in Cycas elongata have not been well characterized. In this study, 13 reference genes (ACT7, TUB, UBQ, EIF4, EF1, CLATHRIN1, PP2A, RPB2, GAPC2, TIP41, MAPK, SAMDC and CYP) were chosen from the transcriptome database of C. elongata, and these genes were evaluated in 8 different organ samples. Three software programs, NormFinder, GeNorm and BestKeeper, were used to validate the stability of the potential reference genes. Results obtained from these three programs suggested that CeGAPC2 and CeRPB2 are the most stable reference genes, while CeACT7 is the least stable one among the 13 tested genes. Further confirmation of the identified reference genes was established by the relative expression of AGAMOUSE gene of C. elongata (CeAG). While our stable reference genes generated consistent expression patterns in eight tissues, we note that our results indicate that an inappropriate reference gene might cause erroneous results. Our systematic analysis for stable reference genes of C. elongata facilitates further gene expression studies and functional analyses of this species.
[Mh] Termos MeSH primário: Cycas/genética
Regulação da Expressão Gênica de Plantas
Genes Essenciais
Genes de Plantas
Reação em Cadeia da Polimerase em Tempo Real/normas
Transcriptoma
[Mh] Termos MeSH secundário: Cycas/metabolismo
Perfilação da Expressão Gênica
Folhas de Planta/genética
Folhas de Planta/metabolismo
Raízes de Plantas/genética
Raízes de Plantas/metabolismo
Caules de Planta/genética
Caules de Planta/metabolismo
Padrões de Referência
Software
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170324
[Lr] Data última revisão:
170324
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160429
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0154384


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[PMID]:27041513
[Au] Autor:Ahmad Kurnin NA; Shah Ismail MH; Yoshida H; Izhar S
[Ad] Endereço:Department of Chemical and Environmental Engineering, Faculty of Engineering, Universiti Putra Malaysia.
[Ti] Título:Recovery of Palm Oil and Valuable Material from Oil Palm Empty Fruit Bunch by Sub-critical Water.
[So] Source:J Oleo Sci;65(4):283-9, 2016.
[Is] ISSN:1347-3352
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Oil palm empty fruit bunch (EFB) is one of the solid wastes produced in huge volume by palm oil mill. Whilst it still contains valuable oil, approximately 22.6 million tons is generated annually and treated as solid waste. In this work, sub-critical water (sub-cw) was used to extract oil, sugar and tar from spikelet of EFB. The spikelet was treated with sub-cw between 180-280°C and a reaction time of 2 and 5 minutes. The highest yield of oil was 0.075 g-oil/g-dry EFB, obtained at 240°C and reaction time of 5 minutes. Astonishingly, oil that was extracted through this method was 84.5% of that obtained through Soxhlet method using hexane. Yield of oil extracted was strongly affected by the reaction temperature and time. Higher reaction temperature induces the dielectric constant of water towards the non-polar properties of solvent; thus increases the oil extraction capability. Meanwhile, the highest yield of sugar was 0.20 g-sugar/g-dry EFB obtained at 220°C. At this temperature, the ion product of water is high enough to enable maximum sub-critical water hydrolysis reaction. This study showed that oil and other valuable material can be recovered using water at sub-critical condition, and most attractive without the use of harmful organic solvent.
[Mh] Termos MeSH primário: Cycas/química
Frutas/química
Óleos Vegetais/isolamento & purificação
Eliminação de Resíduos/métodos
Resíduos Sólidos/análise
Água
[Mh] Termos MeSH secundário: Sacarose na Dieta/isolamento & purificação
Hidrólise
Organofosfatos/isolamento & purificação
Óleo de Palmeira
Alcatrões/isolamento & purificação
Temperatura Ambiente
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Dietary Sucrose); 0 (Organophosphates); 0 (Plant Oils); 0 (Solid Waste); 0 (Tars); 059QF0KO0R (Water); 16165-66-5 (hexylphosphonic acid diethyl ester); 5QUO05548Z (Palm Oil)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160405
[St] Status:MEDLINE
[do] DOI:10.5650/jos.ess15209


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[PMID]:26867733
[Au] Autor:Shimokawa M; Haraguchi T; Minami Y; Yagi F; Hiemori K; Tateno H; Hirabayashi J
[Ad] Endereço:Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University, Kagoshima, Japan and.
[Ti] Título:Two carbohydrate recognizing domains from Cycas revoluta leaf lectin show the distinct sugar-binding specificity-A unique mannooligosaccharide recognition by N-terminal domain.
[So] Source:J Biochem;160(1):27-35, 2016 Jul.
[Is] ISSN:1756-2651
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Cycas revoluta leaf lectin (CRLL) of mannose-recognizing jacalin-related lectin (mJRL) has two tandem repeated carbohydrate recognition domains, and shows the characteristic sugar-binding specificity toward high mannose-glycans, compared with other mJRLs. We expressed the N-terminal domain and C-terminal domain (CRLL-N and CRLL-C) separately, to determine the fine sugar-binding specificity of each domain, using frontal affinity chromatography, glycan array and equilibrium dialysis. The specificity of CRLL toward high mannose was basically derived from CRLL-N, whereas CRLL-C had affinity for α1-6 extended mono-antennary complex-type glycans. Notably, the affinity of CRLL-N was most potent to one of three Man 8 glycans and Man 9 glycan, whereas the affinity of CRLL-C decreased with the increase in the number of extended α1-2 linked mannose residue. The recognition of the Man 8 glycans by CRLL-N has not been found for other mannose recognizing lectins. Glycan array reflected these specificities of the two domains. Furthermore, it was revealed by equilibrium dialysis method that the each domain had two sugar-binding sites, similar with Banlec, banana mannose-binding Jacalin-related lectin.
[Mh] Termos MeSH primário: Cycas/química
Lectinas de Ligação a Manose/química
Folhas de Planta/química
Lectinas de Plantas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Mannose-Binding Lectins); 0 (Plant Lectins)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170130
[Lr] Data última revisão:
170130
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160213
[St] Status:MEDLINE
[do] DOI:10.1093/jb/mvw011


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[PMID]:26637996
[Au] Autor:Wang W; Ma L; Becher H; Garcia S; Kovarikova A; Leitch IJ; Leitch AR; Kovarik A
[Ad] Endereço:School of Biological and Chemical Sciences, Queen Mary University of London, London, E1 4NS, UK.
[Ti] Título:Astonishing 35S rDNA diversity in the gymnosperm species Cycas revoluta Thunb.
[So] Source:Chromosoma;125(4):683-99, 2016 09.
[Is] ISSN:1432-0886
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:In all eukaryotes, the highly repeated 35S ribosomal DNA (rDNA) sequences encoding 18S-5.8S-26S ribosomal RNA (rRNA) typically show high levels of intragenomic uniformity due to homogenisation processes, leading to concerted evolution of 35S rDNA repeats. Here, we compared 35S rDNA divergence in several seed plants using next generation sequencing and a range of molecular and cytogenetic approaches. Most species showed similar 35S rDNA homogeneity indicating concerted evolution. However, Cycas revoluta exhibits an extraordinary diversity of rDNA repeats (nucleotide sequence divergence of different copies averaging 12 %), influencing both the coding and non-coding rDNA regions nearly equally. In contrast, its rRNA transcriptome was highly homogeneous suggesting that only a minority of genes (<20 %) encode functional rRNA. The most common SNPs were C > T substitutions located in symmetrical CG and CHG contexts which were also highly methylated. Both functional genes and pseudogenes appear to cluster on chromosomes. The extraordinary high levels of 35S rDNA diversity in C. revoluta, and probably other species of cycads, indicate that the frequency of repeat homogenisation has been much lower in this lineage, compared with all other land plant lineages studied. This has led to the accumulation of methylation-driven mutations and pseudogenisation. Potentially, the reduced homology between paralogs prevented their elimination by homologous recombination, resulting in long-term retention of rDNA pseudogenes in the genome.
[Mh] Termos MeSH primário: Cycas/genética
DNA de Plantas/genética
DNA Ribossômico/genética
RNA Ribossômico 18S/genética
RNA Ribossômico 5,8S/genética
RNA Ribossômico/genética
[Mh] Termos MeSH secundário: Sequência de Bases
DNA Espaçador Ribossômico/genética
Hibridização in Situ Fluorescente
Polimorfismo de Nucleotídeo Único/genética
Análise de Sequência de DNA
Transcrição Genética/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Plant); 0 (DNA, Ribosomal); 0 (DNA, Ribosomal Spacer); 0 (RNA, Ribosomal); 0 (RNA, Ribosomal, 18S); 0 (RNA, Ribosomal, 5.8S); 0 (RNA, ribosomal, 26S)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170327
[Lr] Data última revisão:
170327
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151207
[St] Status:MEDLINE
[do] DOI:10.1007/s00412-015-0556-3


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[PMID]:26495646
[Au] Autor:Shi HY; Zhu HP; Qu YJ; Yang PJ
[Ti] Título:[Determination of Flavonoids in Cycas revoluta Leaves by Chemiluminescence-Flow Injection Analysis Method].
[So] Source:Zhong Yao Cai;38(3):481-4, 2015 Mar.
[Is] ISSN:1001-4454
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:OBJECTIVE: To detect flavonoids from Cycas revoluta leaves by means of Chemiluminescence-Flow Injection Analysis (CL-FIA). METHODS: Under alkaline condition, a CL-FIA method was established to determine flavonoids from leaves of Cycas revoluta on the basis of inhibiting effect of flavonoids to the Luminol-H2O2-Cu2+ chemiluminescence system and the reversed flow injection technique. RESULTS: In the range of 2. 0 x 10(-6) ~ 1. 0 x 10(-3) mg/mL, the decrease of CL intensity was correlated with flavonoids concentration while the detection limit was 0. 0265 µg/mL. Under the optimized conditions, the flavonoids of Cycas revoluta leaves were detected with its average rate reaching 1. 61% and RSD 1. 32%. CONCLUSION: Through the interference test and compared with the data of CL-FIA and UV, it is concluded that CL-FIA can be used in the analysis and detection of flavonoids from Cycas revoluta leaves.
[Mh] Termos MeSH primário: Cycas/química
Flavonoides/análise
Folhas de Planta/química
[Mh] Termos MeSH secundário: Análise de Injeção de Fluxo
Limite de Detecção
Medições Luminescentes
Luminol
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Flavonoids); 5EXP385Q4F (Luminol)
[Em] Mês de entrada:1512
[Cu] Atualização por classe:151026
[Lr] Data última revisão:
151026
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151027
[St] Status:MEDLINE


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[PMID]:26396078
[Au] Autor:Beach DG; Kerrin ES; Quilliam MA
[Ad] Endereço:Measurement Science and Standards, National Research Council Canada, 1411 Oxford Street, Halifax, NS, B3H 3Z1, Canada. daniel.beach@nrc-cnrc.gc.ca.
[Ti] Título:Selective quantitation of the neurotoxin BMAA by use of hydrophilic-interaction liquid chromatography-differential mobility spectrometry-tandem mass spectrometry (HILIC-DMS-MS/MS).
[So] Source:Anal Bioanal Chem;407(28):8397-409, 2015 Nov.
[Is] ISSN:1618-2650
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The neurotoxin ß-N-methylamino-L-alanine (BMAA) has been reported in cyanobacteria and shellfish, raising concerns about widespread human exposure. However, inconsistent results for BMAA analysis have led to controversy. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is the most appropriate method for analysis of BMAA, but the risk of interference from isomers, other sample components, and the electrospray background is still present. We have investigated differential mobility spectrometry (DMS) as an ion filter to improve selectivity in the hydrophilic interaction liquid chromatographic (HILIC)-MS/MS determination of BMAA. We obtained standards for two BMAA isomers not previously analyzed by HILIC-MS, ß-amino-N-methylalanine and 3,4-diaminobutanoic acid, and the typically used 2,4-diaminobutanoic acid and N-(2-aminoethyl)glycine. DMS separation of BMAA from these isomers was achieved and optimized conditions were used to develop a sensitive and highly selective multidimensional HILIC-DMS-MS/MS method. This work revealed current technical limitations of DMS for trace quantitation, and practical solutions were implemented. Accurate control of low levels of DMS carrier gas modifier was essential, but required external metering. The linearity of our optimized method was excellent from 0.01 to 6 µmol L(-1). The instrumental LOD was 0.4 pg BMAA injected on-column and the estimated method LOD was 20 ng g(-1) dry weight for BMAA in sample matrix. The method was used to analyze cycad plant tissue, a cyanobacterial reference material, and mussel tissues, by use of isotope-dilution quantitation with deuterated BMAA. This confirmed the presence of BMAA and several of its isomers in cycad and mussel tissues, including commercially available mussel tissue reference materials certified for other biotoxins. Graphical Abstract Differential Mobility Spectrometry is used to increases the selectivity of BMAA analysis by HILIC-MS/MS.
[Mh] Termos MeSH primário: Diamino Aminoácidos/análise
Cromatografia Líquida/normas
Contaminação de Alimentos
Neurotoxinas/análise
Espectrometria de Massas em Tandem/normas
[Mh] Termos MeSH secundário: Aminobutiratos/análise
Animais
Bivalves/química
Bivalves/metabolismo
Calibragem
Cromatografia Líquida/métodos
Cianobactérias/química
Cianobactérias/metabolismo
Cycas/química
Cycas/metabolismo
Glicina/análogos & derivados
Glicina/análise
Interações Hidrofóbicas e Hidrofílicas
Limite de Detecção
Padrões de Referência
Espectrometria de Massas em Tandem/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (3,4-diaminobutanoic acid); 0 (Amino Acids, Diamino); 0 (Aminobutyrates); 0 (Neurotoxins); 108SA6URTV (beta-N-methylamino-L-alanine); 24123-14-6 (N-(2-aminoethyl)glycine); TE7660XO1C (Glycine)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:151106
[Lr] Data última revisão:
151106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150924
[St] Status:MEDLINE
[do] DOI:10.1007/s00216-015-9012-8



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