Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.156.577.222.500 [Categoria DeCS]
Referências encontradas : 139 [refinar]
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[PMID]:29390365
[Au] Autor:Ryu SJ; Shin YU; Kang MH; Cho HY; Seong M
[Ad] Endereço:Department of Ophthalmology, Hanyang University College of Medicine, Seongdong-gu, Seoul, Republic of Korea.
[Ti] Título:Bilateral acute myopia and angle closure glaucoma induced by Ma-huang (Ephedra): A case report.
[So] Source:Medicine (Baltimore);96(50):e9257, 2017 Dec.
[Is] ISSN:1536-5964
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:RATIONALE: Cases of bilateral acute angle closure have been reported after use of various drugs. PATIENT CONCERNS: A 52-year-old woman visited the emergency room and complained of acute bilateral ocular pain and decreased vision accompanied by headache, nausea, and vomiting. One day before, she had started a herbal medicine containing Ma-huang for weight loss. On examinations, myopic shift, edematous cornea, increased intraocular pressure, shallow anterior chamber, and thickened choroid on both eyes were observed. DIAGNOSES: Angle closure glaucoma induced by drug (Ma-huang). INTERVENTIONS: To promptly quit the offending drug and apply ocular hypotensives and cycloplegics. OUTCOMES: Her symptoms and signs were relieved after antiglaucoma medications and no significant recurrence has been occurred. LESSONS: Physicians prescribing weight loss medications containing Ma-huang must be aware of the potentially sight-threatening adverse effect of bilateral acute angle closure.
[Mh] Termos MeSH primário: Ephedra sinica/efeitos adversos
Glaucoma de Ângulo Fechado/induzido quimicamente
Miopia/induzido quimicamente
[Mh] Termos MeSH secundário: Doença Aguda
Diagnóstico Diferencial
Feminino
Glaucoma de Ângulo Fechado/tratamento farmacológico
Seres Humanos
Meia-Idade
Miopia/tratamento farmacológico
República da Coreia
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:180203
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000009257


  2 / 139 MEDLINE  
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[PMID]:28717105
[Au] Autor:Lee AY; Jang Y; Hong SH; Chang SH; Park S; Kim S; Kang KS; Kim JE; Cho MH
[Ad] Endereço:Laboratory of Toxicology, Research Institute for Veterinary Science and College of Veterinary Medicine, Seoul National University, Korea.
[Ti] Título:Ephedrine-induced mitophagy via oxidative stress in human hepatic stellate cells.
[So] Source:J Toxicol Sci;42(4):461-473, 2017.
[Is] ISSN:1880-3989
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:The herb Ephedra sinica (also known as Chinese ephedra or Ma Huang), used in traditional Chinese medicine, contains alkaloids identical to ephedrine and pseudoephedrine as its principal active constituents. Recent studies have reported that ephedrine has various side effects in the cardiovascular and nervous systems. In addition, herbal Ephedra, a plant containing many pharmacologically active alkaloids, principally ephedrine, has been reported to cause acute hepatitis. Many studies reported clinical cases, however, the cellular mechanism of liver toxicity by ephedrine remains unknown. In this study, we investigated hepatotoxicity and key regulation of mitophagy in ephedrine-treated LX-2 cells. Ephedrine triggered mitochondrial oxidative stress and depolarization. Mitochondrial swelling and autolysosome were observed in ephedrine-treated cells. Ephedrine also inhibited mitochondrial biogenesis, and the mitochondrial copy number was decreased. Parkin siRNA recovered the ephedrine-induced mitochondrial damage. Excessive mitophagy lead to cell death through imbalance of autophagic flux. Moreover, antioxidants and reducing Parkin level could serve as therapeutic targets for ephedrine-induced hepatotoxicity.
[Mh] Termos MeSH primário: Doença Hepática Induzida por Substâncias e Drogas/etiologia
Efedrina/toxicidade
Células Estreladas do Fígado/efeitos dos fármacos
Mitocôndrias Hepáticas/efeitos dos fármacos
Mitocôndrias Hepáticas/metabolismo
Degradação Mitocondrial/efeitos dos fármacos
Estresse Oxidativo/efeitos dos fármacos
[Mh] Termos MeSH secundário: Antioxidantes/uso terapêutico
Autofagia
Morte Celular
Células Cultivadas
Doença Hepática Induzida por Substâncias e Drogas/terapia
Ephedra sinica/química
Efedrina/isolamento & purificação
Dosagem de Genes/efeitos dos fármacos
Seres Humanos
Lisossomos/efeitos dos fármacos
Mitocôndrias Hepáticas/genética
Mitocôndrias Hepáticas/patologia
Dilatação Mitocondrial/efeitos dos fármacos
Terapia de Alvo Molecular
Biogênese de Organelas
RNA Interferente Pequeno/efeitos dos fármacos
Ubiquitina-Proteína Ligases/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (RNA, Small Interfering); EC 2.3.2.27 (Ubiquitin-Protein Ligases); EC 2.3.2.27 (parkin protein); GN83C131XS (Ephedrine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171005
[Lr] Data última revisão:
171005
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170719
[St] Status:MEDLINE
[do] DOI:10.2131/jts.42.461


  3 / 139 MEDLINE  
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[PMID]:28545666
[Au] Autor:Wang Z; Cui Y; Ding G; Zhou M; Ma X; Hou Y; Jiang M; Liu D; Bai G
[Ad] Endereço:State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300350, China.
[Ti] Título:Mahuannin B an adenylate cyclase inhibitor attenuates hyperhidrosis via suppressing ß -adrenoceptor/cAMP signaling pathway.
[So] Source:Phytomedicine;30:18-27, 2017 Jul 01.
[Is] ISSN:1618-095X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Based on the traditional application of traditional Chinese Medicines (TCMs), Ephedra Herba (EH) is used to cure cold fever by inducing sweating, whereas Ephedra Radix (ER) is used to treat hyperhidrosis. Although they come from the same plant, Ephedra sinica Stapf, but have play opposing roles in clinical applications. EH is known to contain ephedrine alkaloids, which is the driver of the physiological changes in sweating, heart rate and blood pressure. However, the active pharmacological ingredients (APIs) of ER and the mechanisms by which it restricts sweating remain unknown. PURPOSE: The current work aims to discover the hidroschesis APIs from ER, as well as to establish its action mechanism. METHODS: UPLC-Q/TOF-MS, PCA, and heat map were utilized for identifying the differences between EH and ER. HPLC integrated with a ß -adrenoceptor (ß -AR) activity luciferase reporter assay system was used to screen active inhibitors; molecular docking and a series of biological assays centered on ß -AR-related signaling pathways were evaluated to understand the roles of APIs. RESULTS: The opposite effect on sweating of EH and ER can be attributed to the APIs of amphetamine-type alkaloids and flavonoid derivatives. Mahuannin B is an effective anti-hydrotic agent, inhibiting the production of cAMP via suppression of adenylate cyclase (AC) activity. CONCLUSION: The effects of EH and ER on sweat and ß -AR-related signaling pathway are opposite due to different alkaloids and flavonoids of APIs in EH and ER. The present work not only sheds light on the hidroschesis action of mahuannin B, but also presents a potential target of AC in the treatment of hyperhidrosis.
[Mh] Termos MeSH primário: Inibidores de Adenilil Ciclase/farmacologia
Alcaloides/farmacologia
AMP Cíclico/metabolismo
Medicamentos de Ervas Chinesas/farmacologia
Ephedra/química
Flavonoides/farmacologia
Receptores Adrenérgicos beta 2/metabolismo
[Mh] Termos MeSH secundário: Inibidores de Adenilil Ciclase/química
Antagonistas de Receptores Adrenérgicos beta 2/química
Antagonistas de Receptores Adrenérgicos beta 2/farmacologia
Alcaloides/química
Animais
Cromatografia Líquida de Alta Pressão
Avaliação Pré-Clínica de Medicamentos/métodos
Medicamentos de Ervas Chinesas/química
Ephedra sinica/química
Efedrina/farmacologia
Flavonoides/química
Masculino
Camundongos
Simulação de Acoplamento Molecular
Receptores Adrenérgicos
Transdução de Sinais/efeitos dos fármacos
Especificidade da Espécie
Sudorese/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adenylyl Cyclase Inhibitors); 0 (Adrenergic beta-2 Receptor Antagonists); 0 (Alkaloids); 0 (Drugs, Chinese Herbal); 0 (Flavonoids); 0 (Receptors, Adrenergic); 0 (Receptors, Adrenergic, beta-2); 0 (mahuannin B); E0399OZS9N (Cyclic AMP); GN83C131XS (Ephedrine)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170912
[Lr] Data última revisão:
170912
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170527
[St] Status:MEDLINE


  4 / 139 MEDLINE  
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[PMID]:28049947
[Au] Autor:Hiyama H; Ozawa A; Kumazawa H; Takeda O
[Ad] Endereço:Botanical Raw Materials Research Laboratories, Tsumura & Co.
[Ti] Título:Stabilization of Ephedrine Alkaloid Content in Ephedra sinica by Selective Breeding and Stolon Propagation.
[So] Source:Biol Pharm Bull;40(1):43-48, 2017.
[Is] ISSN:1347-5215
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:The Ephedra herb, which has been used in Kampo medicines, originates from terrestrial stems of Ephedra species. It is important to establish cultivation methods and cultivars to secure a stable supply of the Ephedra herb that would meet the quality standards for the ephedrine alkaloids content. In this study, we first grew Ephedra sinica plants derived from seeds in the field for 5 years. Then, for selective breeding of cultivars that could meet the quality standards for the ephedrine alkaloids content, we measured the content of total alkaloids (TAs), ephedrine (Eph), and pseudoephedrine (PEph) in individual plants derived from seedlings and grown for 4 years in the field. The range of the TA content in each individual plant was narrower than that among individual plants grown in the field. Therefore, individual plants were selected according to their TA content, Eph/PEph ratio, and stolon-formation capability. The selected individuals were propagated using stolons, and their TA content was studied for 2 years. In the second year, the TA content in terrestrial stems derived from stolons of the selected individuals was as high as that of their parents. Therefore, it was confirmed that the selected individuals that were propagated using stolons could produce TA reproducibly. This study suggested that selective breeding using stolon propagation is effective for stabilizing Ephedra herb TA content.
[Mh] Termos MeSH primário: Alcaloides/metabolismo
Ephedra sinica/metabolismo
Efedrina/metabolismo
Pseudoefedrina/metabolismo
[Mh] Termos MeSH secundário: Alcaloides/análise
Efedrina/análise
Melhoramento Vegetal
Pseudoefedrina/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkaloids); 7CUC9DDI9F (Pseudoephedrine); GN83C131XS (Ephedrine)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170203
[Lr] Data última revisão:
170203
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170105
[St] Status:MEDLINE
[do] DOI:10.1248/bpb.b16-00531


  5 / 139 MEDLINE  
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[PMID]:27733484
[Au] Autor:Xu J; Yan R
[Ad] Endereço:Department of Foreign Languages, Chaohu University, Chaohu 238000, P.R. China.
[Ti] Título:Determination of the Metabolite of Ephedrine, 4-Hydroxyephedrine, by LC-MS-MS in Rat Urine and Its Application in Excretion Profiles After Oral Administration of Ephedra sinica Stapf and Processing Ephedra sinica Stapf.
[So] Source:J Chromatogr Sci;55(2):162-165, 2017 02.
[Is] ISSN:1945-239X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This study aims to study the changes of metabolism of Ephedra sinica Stapf caused by processing. A sensitive and rapid high-performance liquid chromatographic tandem mass spectrometry (LC-MS-MS) method was developed for the quantitation of the major metabolite of ephedrine, 4-hydroxyephedrine, utilizing lidocaine as the internal standard in rat urine. Urine samples were precipitated with acetonitrile. Chromatographic separation was achieved on an Ultimate C18 analytical column. Detection was performed by a multiple reaction monitoring mode via an electrospray ionization source operating in the positive ionization mode. The method was linear over the concentration range of 0.05-1.0c for all components. The intra- and inter-day precision values were <13.2% and the deviations ranged from -8.4% to 7.5%. The recoveries at three levels were more than 66.2%. The fully validated method was used to study the pharmacokinetic profile of 4-hydroxyephedrine in rat urine to investigate the effects caused by processing.
[Mh] Termos MeSH primário: Cromatografia Líquida/métodos
Medicamentos de Ervas Chinesas/administração & dosagem
Ephedra sinica
Efedrina/análogos & derivados
Espectrometria de Massas em Tandem/métodos
[Mh] Termos MeSH secundário: Administração Oral
Animais
Medicamentos de Ervas Chinesas/farmacocinética
Efedrina/metabolismo
Efedrina/farmacocinética
Efedrina/urina
Modelos Lineares
Ratos
Ratos Sprague-Dawley
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drugs, Chinese Herbal); F49638UBDR (oxilofrine); GN83C131XS (Ephedrine)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170911
[Lr] Data última revisão:
170911
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161014
[St] Status:MEDLINE
[do] DOI:10.1093/chromsci/bmw165


  6 / 139 MEDLINE  
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[PMID]:27220077
[Au] Autor:Schäfer S; Salcher S; Seiter M; Ranninger C; Möst M; Obexer P; Huber CG; Ausserlechner MJ; Schwaiger S; Stuppner H
[Ad] Endereço:Institute of Pharmacy, Pharmacognosy and Center for Molecular Biosciences Innsbruck (CMBI), University of Innsbruck, Innsbruck, Austria.
[Ti] Título:Characterization of the XIAP-Inhibiting Proanthocyanidin Fraction of the Aerial Parts of Ephedra sinica.
[So] Source:Planta Med;82(11-12):973-85, 2016 Jul.
[Is] ISSN:1439-0221
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The X-linked inhibitor of apoptosis protein is a cellular protein that inhibits the activity of mammalian caspases and promotes resistance to apoptosis. The ethanol extract of the aerial parts of Ephedra sinica has been identified to possess inhibitory activity of the X-linked inhibitor of apoptosis protein by an in vitro fluorescence polarization assay using the BIR3 domain of the X-linked inhibitor of apoptosis protein. Bioactivity-guided fractionation identified proanthocyanidin-enriched fractions as the active principles. The most active fraction showed an IC50 value of 27.3 µg/mL (CI95: 25.9-28.9 µg/mL) corresponding to 9.6 µM (CI95: 9.1-10.1 µM) calculated by the use of the determined average molecular weight of 2853.5. Samples were analyzed by a thiolytic degradation/HPLC-MS assay, UHPLC-HRMS, and 1D NMR.The thiolytic degradation/HPLC-MS assay revealed a mean degree of polymerization of 9.5 ± 0.2 units (calculated average MW 2853.5) for the active fraction and 11.4 ± 0.6 units (calculated average MW 3437.0) for the most related inactive fraction. Chemical characterization identified (epi)gallocatechin (76.6 ± 1.0 % active; 80.7 ± 2.7 % inactive sample) and (epi)catechin units as building blocks. Interestingly, the investigated proanthocyanidins turned out to be a complex mixture of double linked A-type (binding 2-O-7″, 4-6″) and single linked B-type units.This study identified oligomeric proanthocyanidins as active principles of E. sinica in vitro by a fluorescence polarization assay and via protein fragment complementation analysis.
[Mh] Termos MeSH primário: Ephedra sinica/química
Extratos Vegetais/farmacologia
Proantocianidinas/farmacologia
Proteínas Inibidoras de Apoptose Ligadas ao Cromossomo X/antagonistas & inibidores
[Mh] Termos MeSH secundário: Polarização de Fluorescência
Células HEK293
Seres Humanos
Componentes Aéreos da Planta/química
Extratos Vegetais/química
Extratos Vegetais/isolamento & purificação
Proantocianidinas/química
Proantocianidinas/isolamento & purificação
Ligação Proteica
Domínios Proteicos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Extracts); 0 (Proanthocyanidins); 0 (X-Linked Inhibitor of Apoptosis Protein); 0 (XIAP protein, human)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170316
[Lr] Data última revisão:
170316
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160525
[St] Status:MEDLINE
[do] DOI:10.1055/s-0042-107253


  7 / 139 MEDLINE  
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[PMID]:27183898
[Au] Autor:Sun Q; Cao H; Zhou Y; Wang X; Jiang H; Gong L; Yang Y; Rong R
[Ad] Endereço:School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, Shandong, China.
[Ti] Título:Qualitative and quantitative analysis of the chemical constituents in Mahuang-Fuzi-Xixin decoction based on high performance liquid chromatography combined with time-of-flight mass spectrometry and triple quadrupole mass spectrometers.
[So] Source:Biomed Chromatogr;30(11):1820-1834, 2016 Nov.
[Is] ISSN:1099-0801
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:High-performance liquid chromatography coupled with time-of-flight mass spectrometry (HPLC-TOF/MS) and high-performance liquid chromatography-triple quadrupole mass spectrometry (HPLC-QQQ/MS/MS) were utilized to clarify the chemical constituents of Mahuang-Fuzi-Xixin Decoction. There are 52 compounds, including alkaloids, amino acids and organic acids were identified or tentatively characterized by their characteristic high resolution mass data by HPLC-QQQ/MS/MS. In the subsequent quantitative analysis, 10 constituents, including methyl ephedrine, aconine, songrine, fuziline, neoline, talatisamine, chasmanine, benzoylmesaconine, benzoylaconine and benzoylhypaconine were simultaneously determined by HPLC-QQQ/MS/MS with multiple reaction monitoring mode. Satisfactory linearity was achieved with wide linear range and fine determination coefficient (r > 0.9992). The relative standard deviations (RSD) of inter- and intra-day precisions were <3%. This method was also validated by repeatability, stability and recovery with RSD <3% respectively. A highly sensitive and efficient method was established for chemical constituents studying, including identification and quantification of Mahuang-Fuzi-Xixin decoction.
[Mh] Termos MeSH primário: Aconitum/química
Alcaloides/análise
Asarum/química
Medicamentos de Ervas Chinesas/química
Ephedra sinica/química
Espectrometria de Massas em Tandem/métodos
[Mh] Termos MeSH secundário: Aminoácidos/análise
Cromatografia Líquida de Alta Pressão/métodos
Limite de Detecção
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Alkaloids); 0 (Amino Acids); 0 (Drugs, Chinese Herbal)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170124
[Lr] Data última revisão:
170124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160518
[St] Status:MEDLINE
[do] DOI:10.1002/bmc.3758


  8 / 139 MEDLINE  
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[PMID]:26968679
[Au] Autor:Lv MY; Sun JB; Wang M; Fan HY; Zhang ZJ; Xu FG
[Ad] Endereço:Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education), China Pharmaceutical University, Nanjing 210009, China; State Key Laboratory of Natural Medicine, China Pharmaceutical University, Nanjing 210009, China.
[Ti] Título:Comparative analysis of volatile oils in the stems and roots of Ephedra sinica via GC-MS-based plant metabolomics.
[So] Source:Chin J Nat Med;14(2):133-140, 2016 Feb.
[Is] ISSN:1875-5364
[Cp] País de publicação:China
[La] Idioma:eng
[Ab] Resumo:With a great difference in therapeutic effects of Mahuang (MH, the stems of Ephedra sinica) and Mahuanggen (MHG, the roots of Ephedra sinica), chemical differences between MH and MHG should be investigated. In the present study, gas chromatography-mass spectrometry (GC-MS)-based plant metabolomics was employed to compare volatile oil profiles of MH and MHG. The antioxidant activities of volatile oils from MH and MHG were also compared. 32 differential chemical markers were identified according to the variable importance in the projection (VIP) value of orthogonal partial least squares discriminant analysis (OPLS-DA) and P value of Mann-Whitney test. Among them, chemical markers of tetramethylpyrazine (TMP) and α-terpineol were quantified. Their contents were much higher in most MH samples compared with MHG. The antioxidant assay demonstrated that MH had significantly higher free radical-scavenging activity than MHG. Although MH and MHG derived from the same medicinal plant, there was much difference in their volatile oil profiles. MH samples had significantly higher content of two reported pharmacologically important chemical markers of TMP and α-terpineol, which may account for their different antioxidant activities.
[Mh] Termos MeSH primário: Medicamentos de Ervas Chinesas/química
Ephedra sinica/química
Óleos Voláteis/química
Raízes de Plantas/química
Caules de Planta/química
[Mh] Termos MeSH secundário: Cromatografia Gasosa-Espectrometria de Massas
Metabolômica
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Drugs, Chinese Herbal); 0 (Oils, Volatile)
[Em] Mês de entrada:1611
[Cu] Atualização por classe:170729
[Lr] Data última revisão:
170729
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160313
[St] Status:MEDLINE


  9 / 139 MEDLINE  
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[PMID]:26835987
[Au] Autor:Wang Q; Shu Z; Xing N; Xu B; Wang C; Sun G; Sun X; Kuang H
[Ad] Endereço:Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China; Key Laboratory of Chinese Materia Medica (Heilo
[Ti] Título:A pure polysaccharide from Ephedra sinica treating on arthritis and inhibiting cytokines expression.
[So] Source:Int J Biol Macromol;86:177-88, 2016 May.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In our previous study, we found that the acidic polysaccharides of Ephedra sinica had immunosuppressive effect to treat rheumatoid arthritis and the pure polysaccharide ESP-B4 was the main composition of the acidic polysaccharides. At present, the exact molecular mechanism of ESP-B4 on treating arthritis is unclear. We are thus evaluating the properties of ESP-B4 on LPS-induced THP-1 pro-monocytic cells and adjuvant-induced arthritis in Wistar rats via TLR4. In vitro, ESP-B4 decreased the production of cytokines induced by LPS. In addition, ESP-B4 reduced the LPS-stimulated nuclear translocation of p65 subunit of NF-κB. Pretreatment with ESP-B4 significantly down-regulated the phosphorylation of MAPKs induced by LPS. Furthermore, in vivo, after 12 days of disease induced by adjuvant, rats were treated with ESP-B4 for 16 days. ESP-B4 significantly improved all parameters of inflammation. ESP-B4 reduced the release of inflammatory factors and cytokines by inhibiting the TLR4 signaling pathway to treat rheumatoid arthritis.
[Mh] Termos MeSH primário: Artrite/tratamento farmacológico
Artrite/metabolismo
Citocinas/genética
Citocinas/metabolismo
Ephedra sinica/química
Expressão Gênica/efeitos dos fármacos
Polissacarídeos/farmacologia
[Mh] Termos MeSH secundário: Transporte Ativo do Núcleo Celular/efeitos dos fármacos
Animais
Artrite/genética
Artrite/patologia
Núcleo Celular/efeitos dos fármacos
Núcleo Celular/metabolismo
Citocinas/biossíntese
Dinoprostona/biossíntese
Seres Humanos
Quinase I-kappa B/metabolismo
Ligantes
Lipopolissacarídeos/farmacologia
Masculino
Camundongos
Proteínas Quinases Ativadas por Mitógeno/metabolismo
Fator 88 de Diferenciação Mieloide/metabolismo
NF-kappa B/metabolismo
Óxido Nítrico/biossíntese
Óxido Nítrico Sintase Tipo II/metabolismo
Fosforilação/efeitos dos fármacos
Polissacarídeos/isolamento & purificação
Polissacarídeos/uso terapêutico
Células RAW 264.7
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Ratos
Ratos Wistar
Transdução de Sinais/efeitos dos fármacos
Receptor 4 Toll-Like/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cytokines); 0 (Ligands); 0 (Lipopolysaccharides); 0 (Myeloid Differentiation Factor 88); 0 (NF-kappa B); 0 (Polysaccharides); 0 (RNA, Messenger); 0 (Toll-Like Receptor 4); 31C4KY9ESH (Nitric Oxide); EC 1.14.13.39 (Nitric Oxide Synthase Type II); EC 2.7.11.10 (I-kappa B Kinase); EC 2.7.11.24 (Mitogen-Activated Protein Kinases); K7Q1JQR04M (Dinoprostone)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160203
[St] Status:MEDLINE


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[PMID]:26832171
[Au] Autor:Kilpatrick K; Pajak A; Hagel JM; Sumarah MW; Lewinsohn E; Facchini PJ; Marsolais F
[Ad] Endereço:Department of Biology, University of Western Ontario, London, ON, N6A 5B7, Canada.
[Ti] Título:Characterization of aromatic aminotransferases from Ephedra sinica Stapf.
[So] Source:Amino Acids;48(5):1209-20, 2016 May.
[Is] ISSN:1438-2199
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Ephedra sinica Stapf (Ephedraceae) is a broom-like shrub cultivated in arid regions of China, Korea and Japan. This plant accumulates large amounts of the ephedrine alkaloids in its aerial tissues. These analogs of amphetamine mimic the actions of adrenaline and stimulate the sympathetic nervous system. While much is known about their pharmacological properties, the mechanisms by which they are synthesized remain largely unknown. A functional genomics platform was established to investigate their biosynthesis. Candidate enzymes were obtained from an expressed sequence tag collection based on similarity to characterized enzymes with similar functions. Two aromatic aminotransferases, EsAroAT1 and EsAroAT2, were characterized. The results of quantitative reverse transcription-polymerase chain reaction indicated that both genes are expressed in young stem tissue, where ephedrine alkaloids are synthesized, and in mature stem tissue. Nickel affinity-purified recombinant EsAroAT1 exhibited higher catalytic activity and was more homogeneous than EsAroAT2 as determined by size-exclusion chromatography. EsAroAT1 was highly active as a tyrosine aminotransferase with α-ketoglutarate followed by α-ketomethylthiobutyrate and very low activity with phenylpyruvate. In the reverse direction, catalytic efficiency was similar for the formation of all three aromatic amino acids using L-glutamate. Neither enzyme accepted putative intermediates in the ephedrine alkaloid biosynthetic pathway, S-phenylacetylcarbinol or 1-phenylpropane-1,2-dione, as substrates.
[Mh] Termos MeSH primário: Ephedra sinica/enzimologia
Proteínas de Plantas/química
Proteínas de Plantas/metabolismo
Transaminases/química
Transaminases/metabolismo
[Mh] Termos MeSH secundário: Biocatálise
Estabilidade Enzimática
Ephedra sinica/química
Ephedra sinica/genética
Ephedra sinica/metabolismo
Efedrina/metabolismo
Cinética
Proteínas de Plantas/genética
Proteínas de Plantas/isolamento & purificação
Transaminases/genética
Transaminases/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Plant Proteins); EC 2.6.1.- (Transaminases); GN83C131XS (Ephedrine)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170302
[Lr] Data última revisão:
170302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160203
[St] Status:MEDLINE
[do] DOI:10.1007/s00726-015-2156-1



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