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Pesquisa : B01.650.940.800.575.462 [Categoria DeCS]
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[PMID]:29206876
[Au] Autor:Baczkiewicz A; Szczecinska M; Sawicki J; Stebel A; Buczkowska K
[Ad] Endereço:Department of Genetics, Faculty of Biology, Adam Mickiewicz University, Poznan, Poland.
[Ti] Título:DNA barcoding, ecology and geography of the cryptic species of Aneura pinguis and their relationships with Aneura maxima and Aneura mirabilis (Metzgeriales, Marchantiophyta).
[So] Source:PLoS One;12(12):e0188837, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Aneura pinguis is a thalloid liverwort species with broad geographical distribution. It is composed of cryptic species, however, the number of cryptic species within A. pinguis is not known. Five cpDNA regions (matK, rbcL, rpoC1, trnH-psbA and trnL-trnF) and the entire nuclear ITS region were studied in 130 samples of A. pinguis from different geographical regions. The relationships between the cryptic species of A. pinguis, A. maxima and A. mirabilis were analyzed. All of the examined samples were clustered into 10 clades corresponding to 10 cryptic species of A. pinguis (marked A to J). Aneura mirabilis and A. maxima were nested among different cryptic species of A. pinguis, which indicates that A. pinguis is a paraphyletic taxon. Subgroups were found in cryptic species A, B, C and E. As single barcodes, all tested DNA regions had 100% discriminant power and fulfilled DNA barcode criteria for species identification; however, the only combination detected in all subgroups was trnL-trnF with trnH-psbA or ITS2. The distances between cryptic species were 11- to 35-fold higher than intraspecific distances. In all analyzed DNA regions, the distances between most pairs of cryptic A. pinguis species were higher than between A. maxima and A. mirabilis. All cryptic species of A. pinguis clearly differed in their habitat preferences, which suggests that habitat adaptation could be the main driving force behind cryptic speciation within this taxon.
[Mh] Termos MeSH primário: Código de Barras de DNA Taxonômico
Ecologia
Genes de Plantas
Geografia
Hepatófitas/genética
[Mh] Termos MeSH secundário: Haplótipos
Hepatófitas/classificação
Filogenia
Reação em Cadeia da Polimerase
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171229
[Lr] Data última revisão:
171229
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171206
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0188837


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[PMID]:28409637
[Au] Autor:Ali T; Inagaki M; Chai HB; Wieboldt T; Rapplye C; Rakotondraibe LH
[Ti] Título:Halogenated Compounds from Directed Fermentation of Penicillium concentricum, an Endophytic Fungus of the Liverwort Trichocolea tomentella.
[So] Source:J Nat Prod;80(5):1397-1403, 2017 May 26.
[Is] ISSN:1520-6025
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:One new chlorinated xanthone, 6-chloro-3,8-dihydroxy-1-methylxanthone (1), a new 2-bromo-gentisyl alcohol (2), and a mixture of 6-epimers of 6-dehydroxy-6-bromogabosine C (3a and 3b), together with 19 previously identified compounds, epoxydon (4), norlichexanthone (5), 2-chlorogentisyl alcohol (6), hydroxychlorogentisyl quinone (7), 6-dehydroxy-6α-chlorogabosine C (8a), 6-dehydroxy-6ß-chlorogabosine C (8b), gentisyl alcohol (9), gentisyl quinone (10), (R,S)-1-phenyl-1,2-ethanediol (11), dehydrodechlorogriseofulvin (12), dechlorogriseofulvin (13), dehydrogriseofulvin (14), griseofulvin (15), ethylene glycol benzoate (16), alternariol (17), griseoxanthone C (18), drimiopsin H (19), griseophenone C (20), and griseophenone B (21), were isolated from cultures of Penicillium concentricum, a fungal endophyte of the liverwort Trichocolea tomentella. The structures of the new compounds (1, 2, 3a, and 3b) were elucidated by interpretation of spectroscopic data including one- and two-dimensional NMR techniques. Among these, compounds 2-4 displayed modest cytotoxicity to the MCF-7 hormone-dependent breast cancer cell line with IC values of 8.4, 9.7, and 5.7 µM, respectively, whereas compound 9 exhibited selective cytotoxicity against the HT-29 colon cancer cell line with an IC value of 6.4 µM. During this study we confirmed that the brominated gentisyl alcohol (2) was formed by chemical conversion of 4 during bromide salt addition to culture media.
[Mh] Termos MeSH primário: Álcoois Benzílicos/isolamento & purificação
Álcoois Benzílicos/farmacologia
Neoplasias do Colo/tratamento farmacológico
Compostos de Epóxi/isolamento & purificação
Compostos de Epóxi/farmacologia
Etilenoglicóis/isolamento & purificação
Etilenoglicóis/farmacologia
Hepatófitas/química
Espectroscopia de Ressonância Magnética/métodos
Penicillium/química
Xantonas/farmacologia
[Mh] Termos MeSH secundário: Álcoois Benzílicos/química
Neoplasias do Colo/química
Compostos de Epóxi/química
Etilenoglicóis/química
Fermentação
Células HT29
Halogenação
Seres Humanos
Concentração Inibidora 50
Células MCF-7
Estrutura Molecular
Xantonas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (2-bromo-gentisyl alcohol); 0 (Benzyl Alcohols); 0 (Epoxy Compounds); 0 (Ethylene Glycols); 0 (Xanthones); 0 (epoxydon); 0 (norlichexanthone); 2ZAC511UK8 (styrene glycol); T8T2WY38GH (2,5-dihydroxybenzyl alcohol)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170415
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jnatprod.6b01069


  3 / 360 MEDLINE  
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[PMID]:28381710
[Au] Autor:Kuroda T; Ogawa W
[Ad] Endereço:Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University.
[Ti] Título:Search for Novel Antibacterial Compounds and Targets.
[So] Source:Yakugaku Zasshi;137(4):383-388, 2017.
[Is] ISSN:1347-5231
[Cp] País de publicação:Japan
[La] Idioma:jpn
[Ab] Resumo:Drug-resistant bacteria including methicillin-resistant Staphylococcus aureus (MRSA), multidrug-resistant Pseudomonas aeruginosa, and vancomycin-resistant enterococci (VRE) have been spreading; however, the development of new antibacterial drugs has not progressed accordingly. Novel antibacterial drugs or their candidate seeds need to be developed for effective antibiotic therapy. Under these conditions, the search for novel compounds and novel targets is important. In Okayama University, as a part of the Drug Discovery for Intractable Infectious Diseases project, we are proceeding with the development of antibacterial drugs for the treatment of drug-resistant bacterial infections. We found that riccardin C (a natural product of liverwort) and 6,6'-dihydroxythiobinupharidine (from the crude drug Senkotsu) exhibited strong antibacterial activities, particularly against Gram-positive bacteria. We showed that riccardin C induced cell membrane leakage and that 6,6'-dihydroxythiobinupharidine inhibited DNA topoisomerase IV. Moreover, 6,6'-dihydroxythiobinupharidine exerted synergistic effects with already known anti-MRSA drugs as well as with vancomycin for VRE.
[Mh] Termos MeSH primário: Alcaloides
Antibacterianos
Descoberta de Drogas
Farmacorresistência Bacteriana Múltipla
Éteres Cíclicos
[Mh] Termos MeSH secundário: Alcaloides/farmacologia
Antibacterianos/farmacologia
Produtos Biológicos
Membrana Celular/metabolismo
DNA Topoisomerase IV/antagonistas & inibidores
Éteres Cíclicos/farmacologia
Hepatófitas
Japão
Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos
Enterococos Resistentes à Vancomicina/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Alkaloids); 0 (Anti-Bacterial Agents); 0 (Biological Products); 0 (Ethers, Cyclic); 0 (riccardin C); 30343-70-5 (dihydroxythiobinupharidine); EC 5.99.1.- (DNA Topoisomerase IV)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170407
[St] Status:MEDLINE
[do] DOI:10.1248/yakushi.16-00235-3


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[PMID]:28219451
[Au] Autor:Konglerd P; Reeb C; Jansson F; Kaandorp JA
[Ad] Endereço:Computational Science Lab, University of Amsterdam, Amsterdam, The Netherlands.
[Ti] Título:Quantitative morphological analysis of 2D images of complex-shaped branching biological growth forms: the example of branching thalli of liverworts.
[So] Source:BMC Res Notes;10(1):103, 2017 Feb 20.
[Is] ISSN:1756-0500
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Many organisms such as plants can be characterized as complex-shaped branching forms. The morphological quantification of the forms is a support for a number of areas such as the effects of environmental factors and species discrimination. To date, there is no software package suitable for our dataset representing the forms. We therefore formulate methods for extracting morphological measurements from images of the forms. RESULTS: As a case study we analyze two-dimensional images of samples from four groups belonging to three species of thalloid liverworts, genus Riccardia. The images are pre-processed and converted into binary images, then skeletonized to obtain a skeleton image, in which features such as junctions and terminals are detected. Morphological measurements known to characterize and discriminate the species in the samples such as junction thickness, branch thickness, terminal thickness, branch length, branch angle, and terminal spacing are then quantified. The measurements are used to distinguish among the four groups of Riccardia and also between the two groups of Riccardia amazonica collected in different locations, Africa and South America. Canonical discriminant analysis results show that those measurements are able to discriminate among the four groups. Additionally, it is able to discriminate R. amazonica collected in Africa from those collected in South America. CONCLUSIONS: This paper presents general automated methods implemented in our software for quantifying two-dimensional images of complex branching forms. The methods are used to compute a series of morphological measurements. We found significant results to distinguish Riccardia species by using the measurements. The methods are also applicable for analyzing other branching organisms. Our software is freely available under the GNU GPL.
[Mh] Termos MeSH primário: Hepatófitas/anatomia & histologia
Processamento de Imagem Assistida por Computador/métodos
Fenômenos Fisiológicos Vegetais
[Mh] Termos MeSH secundário: África
América do Sul
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170302
[Lr] Data última revisão:
170302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170222
[St] Status:MEDLINE
[do] DOI:10.1186/s13104-017-2424-0


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[PMID]:28057327
[Au] Autor:Yokota T; Ohnishi T; Shibata K; Asahina M; Nomura T; Fujita T; Ishizaki K; Kohchi T
[Ad] Endereço:Department of Biosciences, Teikyo University, 1-1 Toyosatodai, Utsunomiya 320-8551, Japan. Electronic address: yokota@nasu.bio.teikyo-u.ac.jp.
[Ti] Título:Occurrence of brassinosteroids in non-flowering land plants, liverwort, moss, lycophyte and fern.
[So] Source:Phytochemistry;136:46-55, 2017 Apr.
[Is] ISSN:1873-3700
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Endogenous brassinosteroids (BRs) in non-flowering land plants were analyzed. BRs were found in a liverwort (Marchantia polymorpha), a moss (Physcomitrella patens), lycophytes (Selaginella moellendorffii and S. uncinata) and 13 fern species. A biologically active BR, castasterone (CS), was identified in most of these non-flowering plants but another biologically active BR, brassinolide, was not. It may be distinctive that levels of CS in non-flowering plants were orders of magnitude lower than those in flowering plants. 22-Hydroxycampesterol and its metabolites were identified in most of the non-flowering plants suggesting that the biosynthesis of BRs via 22-hydroxylation of campesterol occurs as in flowering plants. Phylogenetic analyses indicated that M. polymorpha, P. patens and S. moellendorffii have cytochrome P450s in the CYP85 clans which harbors BR biosynthesis enzymes, although the P450 profiles are simpler as compared with Arabidopsis and rice. Furthermore, these basal land plants were found to have multiple P450s in the CYP72 clan which harbors enzymes to catabolize BRs. These findings indicate that green plants were able to synthesize and inactivate BRs from the land-transition stage.
[Mh] Termos MeSH primário: Brassinosteroides/isolamento & purificação
Cycadopsida/química
[Mh] Termos MeSH secundário: Arabidopsis/química
Brassinosteroides/química
Brassinosteroides/metabolismo
Briófitas/química
Bryopsida/química
Sistema Enzimático do Citocromo P-450/metabolismo
Gleiquênias/química
Hepatófitas/química
Marchantia/química
Oryza/química
Filogenia
Selaginellaceae/química
Esteroides Heterocíclicos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Brassinosteroids); 0 (Steroids, Heterocyclic); 9035-51-2 (Cytochrome P-450 Enzyme System); Y9IQ1L53OX (brassinolide)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170107
[St] Status:MEDLINE


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[PMID]:28007566
[Au] Autor:Renner MAM; Heslewood MM; Patzak SDF; Schäfer-Verwimp A; Heinrichs J
[Ad] Endereço:Royal Botanic Gardens and Domain Trust, Mrs. Macquaries Road, Sydney, NSW 2000, Australia. Electronic address: matt.renner@rbgsyd.nsw.gov.au.
[Ti] Título:By how much do we underestimate species diversity of liverworts using morphological evidence? An example from Australasian Plagiochila (Plagiochilaceae: Jungermanniopsida).
[So] Source:Mol Phylogenet Evol;107:576-593, 2017 Feb.
[Is] ISSN:1095-9513
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:As a framework for revisionary study of the leafy liverwort Plagiochila in Australia, two methods for species delimitation on molecular sequence data, General Mixed Yule Coalescence model (GMYC) and Automatic Barcode Gap Discovery (ABGD) were applied to a dataset including 265 individuals from Australia, New Zealand, and the Pacific. Groups returned by GMYC and ABGD were incongruent in some lineages, and ABGD tended to lump groups. This may reflect underlying heterogeneity in the history of diversification within different lineages of Plagiochila. GMYC from trees calculated using three different molecular clocks were compared, in some lineages different primary species hypotheses were returned by analyses of trees estimated under different clock models, suggesting clock model selection should be a routine component of phylogeny reconstruction for tree-based species delimitation methods, such as GMYC. Our results suggest that a minimum of 71 Plagiochilaceae species occur in Australasia, 16 more than currently accepted for the region, comprising 8 undetermined species and 8 synonyms requiring reinstatement. Despite modern taxonomic investigation over a four decade period, (1) real diversity is 29% higher than currently recognized; and (2) 12 of 33, or 36%, of currently accepted and previously untested Australasian species have circumscription issues, including polyphyly, paraphyly, internal phylogenetic structure, or combinations of two or more of these issues. These both reflect the many challenges associated with grouping decisions based solely on morphological data in morphologically simple yet polymorphic plant lineages. Our results highlight again the critical need for combined molecular-morphological datasets as a basis for resolving robust species hypotheses in species-rich bryophyte lineages.
[Mh] Termos MeSH primário: Variação Genética
Hepatófitas/classificação
Hepatófitas/genética
[Mh] Termos MeSH secundário: Australásia
Sequência de Bases
Código de Barras de DNA Taxonômico
Filogenia
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161224
[St] Status:MEDLINE


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[PMID]:27875778
[Au] Autor:Lee N; Choi G
[Ad] Endereço:Department of Biological Sciences, KAIST, Daejeon 34141, Republic of Korea.
[Ti] Título:Phytochrome-interacting factor from Arabidopsis to liverwort.
[So] Source:Curr Opin Plant Biol;35:54-60, 2017 Feb.
[Is] ISSN:1879-0356
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Phytochromes are red and far-red light photoreceptors that regulate the responses of plants to light throughout their life cycles. Phytochromes do this in part by inhibiting the function of a group of basic helix-loop-helix transcription factors called phytochrome-interacting factors (PIFs). Arabidopsis has eight PIFs that function sometimes redundantly and sometimes distinctively depending on their expression patterns and protein stability, as well as on variations in the promoters they target in vivo. PIF-like proteins exist in other seed plants and non-vascular plants where they also regulate light responses. The mechanism by which phytochrome regulates light responses by promoting the degradation of the PIFs is conserved in liverwort, suggesting it must have evolved some time before the last common ancestor shared by seed plants and non-vascular plants.
[Mh] Termos MeSH primário: Fatores de Transcrição Hélice-Alça-Hélice Básicos/genética
Regulação da Expressão Gênica de Plantas
Fitocromo/genética
Proteínas de Plantas/genética
[Mh] Termos MeSH secundário: Arabidopsis
Proteínas de Arabidopsis/genética
Proteínas de Arabidopsis/metabolismo
Fatores de Transcrição Hélice-Alça-Hélice Básicos/metabolismo
Hepatófitas
Fitocromo/metabolismo
Proteínas de Plantas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (Basic Helix-Loop-Helix Transcription Factors); 0 (Plant Proteins); 11121-56-5 (Phytochrome)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161123
[St] Status:MEDLINE


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[PMID]:27664347
[Au] Autor:Patiño J; Wang J; Renner MAM; Gradstein SR; Laenen B; Devos N; Shaw AJ; Vanderpoorten A
[Ad] Endereço:Island Ecology and Evolution Research Group, Instituto de Productos Naturales y Agrobiología (IPNA-CSIC), Astrofísico Francisco Sánchez 3, La Laguna, Tenerife, Canary Islands 38206, Spain; Department of Biology, Ecology and Evolution, Institute of Botany, University of Liège, Liège, Belgium; Azorean
[Ti] Título:Range size heritability and diversification patterns in the liverwort genus Radula.
[So] Source:Mol Phylogenet Evol;106:73-85, 2017 Jan.
[Is] ISSN:1095-9513
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Why some species exhibit larger geographical ranges than others, and to what extent does variation in range size affect diversification rates, remains a fundamental, but largely unanswered question in ecology and evolution. Here, we implement phylogenetic comparative analyses and ancestral area estimations in Radula, a liverwort genus of Cretaceous origin, to investigate the mechanisms that explain differences in geographical range size and diversification rates among lineages. Range size was phylogenetically constrained in the two sub-genera characterized by their almost complete Australasian and Neotropical endemicity, respectively. The congruence between the divergence time of these lineages and continental split suggests that plate tectonics could have played a major role in their present distribution, suggesting that a strong imprint of vicariance can still be found in extant distribution patterns in these highly mobile organisms. Amentuloradula, Volutoradula and Metaradula species did not appear to exhibit losses of dispersal capacities in terms of dispersal life-history traits, but evidence for significant phylogenetic signal in macroecological niche traits suggests that niche conservatism accounts for their restricted geographic ranges. Despite their greatly restricted distribution to Australasia and Neotropics respectively, Amentuloradula and Volutoradula did not exhibit significantly lower diversification rates than more widespread lineages, in contrast with the hypothesis that the probability of speciation increases with range size by promoting geographic isolation and increasing the rate at which novel habitats are encountered. We suggest that stochastic long-distance dispersal events may balance allele frequencies across large spatial scales, leading to low genetic structure among geographically distant areas or even continents, ultimately decreasing the diversification rates in highly mobile, widespread lineages.
[Mh] Termos MeSH primário: Hepatófitas/classificação
[Mh] Termos MeSH secundário: Biodiversidade
Evolução Biológica
Ecossistema
Fenótipo
Filogenia
Filogeografia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170927
[Lr] Data última revisão:
170927
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160925
[St] Status:MEDLINE


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[PMID]:27815071
[Au] Autor:Gao S; Yu HN; Wu YF; Liu XY; Cheng AX; Lou HX
[Ad] Endereço:Key Laboratory of Chemical Biology of Natural Products, Ministry of Education, School of Pharmaceutical Sciences, Shandong University, Jinan, 250012, China.
[Ti] Título:Cloning and functional characterization of a phenolic acid decarboxylase from the liverwort Conocephalum japonicum.
[So] Source:Biochem Biophys Res Commun;481(3-4):239-244, 2016 Dec 09.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Some commercially important vinyl derivatives are produced by the decarboxylation of phenolic acids. Enzymatically, this process can be achieved by phenolic acid decarboxylases (PADs), which are able to act on phenolic acid substrates such as ferulic and p-coumaric acid. Although many microbial PADs have been characterized, little is known regarding their plant homologs. Transcriptome sequencing in the liverworts has identified seven putative PADs, which share a measure of sequence identity with microbial PADs, but are typically much longer proteins. Here, a PAD-encoding gene was isolated from the liverwort species Conocephalum japonicum. The 1197 nt CjPAD cDNA sequence was predicted to be translated into a 398 residue protein. When the gene was heterologously expressed in Escherichia coli, its product exhibited a high level of PAD activity when provided with either p-coumaric or ferulic acid as substrate, along with the conversion of caffeic acid and sinapic acid to their corresponding decarboxylated products. Both N- and C-terminal truncation derivatives were non-functional. The transient expression in tobacco of a GFP/CjPAD fusion gene demonstrated that the CjPAD protein is expressed in the cytoplasm. It is first time a PAD was characterized from plants and the present investigation provided a candidate gene for catalyzing the formation of volatile phenols.
[Mh] Termos MeSH primário: Carboxiliases/genética
Carboxiliases/metabolismo
Hepatófitas/enzimologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Biocatálise
Carboxiliases/química
Cromatografia Líquida de Alta Pressão
Clonagem Molecular
Proteínas de Fluorescência Verde/metabolismo
Epiderme Vegetal/citologia
Proteínas Recombinantes de Fusão/metabolismo
Proteínas Recombinantes/metabolismo
Alinhamento de Sequência
Análise de Sequência de Proteína
Frações Subcelulares/metabolismo
Especificidade por Substrato
Tabaco/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Recombinant Fusion Proteins); 0 (Recombinant Proteins); 147336-22-9 (Green Fluorescent Proteins); EC 4.1.1.- (Carboxy-Lyases); EC 4.1.1.- (phenolic acid decarboxylase)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170522
[Lr] Data última revisão:
170522
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161106
[St] Status:MEDLINE


  10 / 360 MEDLINE  
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[PMID]:27622766
[Au] Autor:Rosato M; Kovarík A; Garilleti R; Rosselló JA
[Ad] Endereço:Jardín Botánico, ICBiBE-Unidad Asociada CSIC, Universidad de Valencia, c/Quart 80, E-46008, Valencia, Spain.
[Ti] Título:Conserved Organisation of 45S rDNA Sites and rDNA Gene Copy Number among Major Clades of Early Land Plants.
[So] Source:PLoS One;11(9):e0162544, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Genes encoding ribosomal RNA (rDNA) are universal key constituents of eukaryotic genomes, and the nuclear genome harbours hundreds to several thousand copies of each species. Knowledge about the number of rDNA loci and gene copy number provides information for comparative studies of organismal and molecular evolution at various phylogenetic levels. With the exception of seed plants, the range of 45S rDNA locus (encoding 18S, 5.8S and 26S rRNA) and gene copy number variation within key evolutionary plant groups is largely unknown. This is especially true for the three earliest land plant lineages Marchantiophyta (liverworts), Bryophyta (mosses), and Anthocerotophyta (hornworts). In this work, we report the extent of rDNA variation in early land plants, assessing the number of 45S rDNA loci and gene copy number in 106 species and 25 species, respectively, of mosses, liverworts and hornworts. Unexpectedly, the results show a narrow range of ribosomal locus variation (one or two 45S rDNA loci) and gene copies not present in vascular plant lineages, where a wide spectrum is recorded. Mutation analysis of whole genomic reads showed higher (3-fold) intragenomic heterogeneity of Marchantia polymorpha (Marchantiophyta) rDNA compared to Physcomitrella patens (Bryophyta) and two angiosperms (Arabidopsis thaliana and Nicotiana tomentosifomis) suggesting the presence of rDNA pseudogenes in its genome. No association between phylogenetic position, taxonomic adscription and the number of rDNA loci and gene copy number was found. Our results suggest a likely evolutionary rDNA stasis during land colonisation and diversification across 480 myr of bryophyte evolution. We hypothesise that strong selection forces may be acting against ribosomal gene locus amplification. Despite showing a predominant haploid phase and infrequent meiosis, overall rDNA homogeneity is not severely compromised in bryophytes.
[Mh] Termos MeSH primário: DNA de Plantas/genética
Embriófitas/classificação
Embriófitas/genética
[Mh] Termos MeSH secundário: Anthocerotophyta/classificação
Anthocerotophyta/genética
Arabidopsis/classificação
Arabidopsis/genética
Briófitas/classificação
Briófitas/genética
Cromossomos de Plantas/genética
Sequência Conservada
Análise Citogenética
DNA Ribossômico/genética
Evolução Molecular
Dosagem de Genes
Genes de Plantas
Hepatófitas/classificação
Hepatófitas/genética
Hibridização in Situ Fluorescente
Filogenia
RNA de Plantas/genética
RNA Ribossômico/genética
Reação em Cadeia da Polimerase em Tempo Real
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Plant); 0 (DNA, Ribosomal); 0 (RNA, Plant); 0 (RNA, Ribosomal); 0 (RNA, ribosomal, 45S)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170808
[Lr] Data última revisão:
170808
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160914
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0162544



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