Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.063.222.222 [Categoria DeCS]
Referências encontradas : 84 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 9 ir para página                      

  1 / 84 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28325830
[Au] Autor:Sessa EB; Juslén A; Väre H; Chambers SM
[Ad] Endereço:Department of Biology, University of Florida, Gainesville, Florida 32611 USA emilysessa@ufl.edu.
[Ti] Título:Into Africa: Molecular phylogenetics and historical biogeography of sub-Saharan African woodferns ( ).
[So] Source:Am J Bot;104(3):477-486, 2017 Mar.
[Is] ISSN:1537-2197
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PREMISE OF THE STUDY: Our goal was to infer the phylogenetic relationships and historical biogeography of the genus with a focus on taxa in sub-Saharan Africa and neighboring islands. In general, little is known about the relationships between African fern species and their congeners in other geographic regions, and our aim was to determine whether the sub-Saharan African species of are monophyletic and evolved within Africa or arrived there via repeated dispersals into Africa from other regions. METHODS: We obtained sequence data for five chloroplast markers from 214 species of and 18 outgroups. We performed phylogenetic and molecular dating analyses using a Bayesian relaxed clock method in BEAST with fossil and secondary calibration points and estimated ancestral ranges for the genus globally by comparing multiple models in BioGeoBEARS. KEY RESULTS: We found that 22 of 27 accessions of sub-Saharan African belong to a large clade of 31 accessions that also includes taxa from Indian and Atlantic Ocean islands. Additional accessions of taxa from our regions of interest have Asian, Hawaiian, European, or North American species as their closest relatives. CONCLUSIONS: The majority of sub-Saharan African species are descended from a shared common ancestor that dispersed to Africa from Asia approximately 10 Ma. There have been subsequent dispersal events from the African mainland to islands in the Atlantic and Indian Oceans, including Madagascar. Several additional species are estimated to have descended from ancestors that reached Africa via separate events over the last roughly 20 million years.
[Mh] Termos MeSH primário: Dryopteris/genética
[Mh] Termos MeSH secundário: África ao Sul do Saara
Teorema de Bayes
Dryopteris/classificação
Dryopteris/fisiologia
Evolução Molecular
Fósseis
Filogenia
Filogeografia
Dispersão Vegetal
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170822
[Lr] Data última revisão:
170822
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170323
[St] Status:MEDLINE
[do] DOI:10.3732/ajb.1600392


  2 / 84 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28323385
[Au] Autor:Chen NH; Qian YR; Li W; Zhang YB; Zhou YD; Li GQ; Li YL; Wang GC
[Ad] Endereço:Institute of Traditional Chinese Medicine & Natural Products, College of Pharmacy, Jinan University, Guangzhou, 510632, P. R. China.
[Ti] Título:Six New Acylphloroglucinols from Dryopteris championii.
[So] Source:Chem Biodivers;14(7), 2017 Jul.
[Is] ISSN:1612-1880
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Six new acylphloroglucinols (1 - 6) were isolated from Dryopteris championii. Their structures were established on the basis of extensive analysis of spectroscopic data and comparison with reported data. The antibacterial activities of the isolates were evaluated against Staphylococcus aureus, Escherichia coli, Bacillus subtilis, and Dickeya zeae.
[Mh] Termos MeSH primário: Antibacterianos/isolamento & purificação
Dryopteris/química
Floroglucinol/isolamento & purificação
[Mh] Termos MeSH secundário: Antibacterianos/química
Bactérias/efeitos dos fármacos
Estrutura Molecular
Floroglucinol/química
Floroglucinol/farmacologia
Extratos Vegetais/química
Extratos Vegetais/farmacologia
Análise Espectral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Plant Extracts); DHD7FFG6YS (Phloroglucinol)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170322
[St] Status:MEDLINE
[do] DOI:10.1002/cbdv.201700001


  3 / 84 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28282885
[Au] Autor:Wang J; Yan YT; Fu SZ; Peng B; Bao LL; Zhang YL; Hu JH; Zeng ZP; Geng DH; Gao ZP
[Ad] Endereço:School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China. juan_wangli@163.com.
[Ti] Título:Anti-Influenza Virus (H5N1) Activity Screening on the Phloroglucinols from Rhizomes of Dryopteris crassirhizoma.
[So] Source:Molecules;22(3), 2017 Mar 08.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:For screening the active phloroglucinols on influenza virus (H5N1) from NaKai, a database was established including twenty-three phloroglucinols that had been isolated from . Their inhibitory effect on the neuraminidase (NA) of influenza virus H5N1 was screened by molecular docking. As a result, three candidates were selected. The rhizomes of were subjected to isolation and purification processes to obtain the inhibitor candidates. Thirteen phloroglucinols were obtained, including three selected candidates and two new phloroglucinols. The five phloroglucinols were investigated for their inhibitory activity on NA in vitro. The results showed that dryocrassin ABBA and filixic acid ABA exhibited inhibitory effects on NA with IC as 18.59 ± 4.53 and 29.57 ± 2.48 µM, respectively, and the other three phloroglucinols showed moderate inhibitory activity. Moreover, the anti-influenza virus (H5N1) activity and cytotoxicity of dryocrassin ABBA and filixic acid ABA were tested on Madin-Darby canine kidney (MDCK) cells with the cell counting kit-8 (CCK8) method. The results confirmed that dryocrassin ABBA exhibited an inhibitory activity with low cytotoxicity (TC > 400 µM) against influenza virus (H5N1) which will have to be investigated in further detail. In conclusion, phloroglucinols from were shown to have anti-influenza virus activity, and especially dryocrassin ABBA, one of the phloroglucinols, may have the potential to control influenza virus (H5N1) infection.
[Mh] Termos MeSH primário: Antivirais/química
Antivirais/farmacologia
Dryopteris/química
Vírus da Influenza A Subtipo H5N1/efeitos dos fármacos
Floroglucinol/química
Floroglucinol/farmacologia
Rizoma/química
[Mh] Termos MeSH secundário: Animais
Sítios de Ligação
Domínio Catalítico
Ativação Enzimática/efeitos dos fármacos
Seres Humanos
Espectroscopia de Ressonância Magnética
Testes de Sensibilidade Microbiana
Modelos Moleculares
Conformação Molecular
Simulação de Acoplamento Molecular
Neuraminidase/antagonistas & inibidores
Neuraminidase/química
Ligação Proteica
Relação Quantitativa Estrutura-Atividade
Espectrometria de Massas por Ionização por Electrospray
Proteínas Virais/antagonistas & inibidores
Proteínas Virais/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiviral Agents); 0 (Viral Proteins); DHD7FFG6YS (Phloroglucinol); EC 3.2.1.18 (NA protein, influenza A virus); EC 3.2.1.18 (Neuraminidase)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170518
[Lr] Data última revisão:
170518
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170312
[St] Status:MEDLINE


  4 / 84 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28117728
[Au] Autor:Zhong ZC; Zhao DD; Liu ZD; Jiang S; Zhang YL
[Ad] Endereço:Food Science Department of XiZang Agriculture and Animal Husbandry College, Xizang 860000, China. zxzzc@163.com.
[Ti] Título:A New Human Cancer Cell Proliferation Inhibition Sesquiterpene, Dryofraterpene A, from Medicinal Plant Dryopteris fragrans (L.) Schott.
[So] Source:Molecules;22(1), 2017 Jan 21.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The global burden of cancer continues to increase largely with the aging and growth of the world population. The purpose of the present work was to find new anticancer molecules from a natural source. We utilized chromatographic methods to isolate compounds from medicinal plant (L.) Schott. The structure of the new compounds was determined by spectroscopic and spectrometric data (1D NMR, 2D NMR, and EMI-MS). Their anti-proliferation effects against five human cancer cell lines including A549, MCF7, HepG2, HeLa, and PC-3 were evaluated by CCK-8 andlactate dehydrogenase (LDH) assay. A new sesquiterpene, (7 , 10 )-2,3-dihydroxy-calamenene-15-carboxylic acid methyl ester ( ), and two known compounds ( and ) were isolated. The new sesquiterpene was named dryofraterpene A and significantly inhibited cancer cell proliferation without any obvious necrosis below a 10 µM concentration. In conclusion, a novel anticancer sesquiterpene together with two known compounds was isolated, which might be a promising lead compound for the treatment of cancer.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/química
Antineoplásicos Fitogênicos/farmacologia
Proliferação Celular/efeitos dos fármacos
Dryopteris/química
Sesquiterpenos/química
Sesquiterpenos/farmacologia
[Mh] Termos MeSH secundário: Células A549
Linhagem Celular Tumoral
Células HeLa
Células Hep G2
Seres Humanos
Células MCF-7
Estrutura Molecular
Extratos Vegetais/química
Plantas Medicinais/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Plant Extracts); 0 (Sesquiterpenes); 0 (dryofraterpene A)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170505
[Lr] Data última revisão:
170505
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170125
[St] Status:MEDLINE


  5 / 84 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:27558683
[Au] Autor:Ali M; Rauf A; Hadda TB; Bawazeer S; Abu-Izneid T; Khan H; Raza M; Khan SA; Shah SU; Pervez S; Patel S; Orhan IE
[Ti] Título:Mechanisms Underlying Anti-hyperalgesic Properties of Kaempferol-3,7- di-O-α-L-rhamnopyranoside Isolated from Dryopteris cycadina.
[So] Source:Curr Top Med Chem;17(4):383-390, 2017.
[Is] ISSN:1873-4294
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The present study was designed to evaluate the anti-hyperalgesic effect of kaempferol-3,7-di-O-α-L-rhamnopyranoside isolated from the ethyl acetate soluble part of Dryopteris cycadina. Pretreatment of the compound at the doses of 2.5, 5, and 10 mg/kg caused a significant reduction in abdominal constrictions in acetic acid-induced writhing test with maximum effect of 63.03% (P < 0.001) at 10 mg/kg i.p. When subjected in formalin test, it evoked a marked antinociceptive effect in both phases in a dose-dependent manner. The maximum (p < 0.01) pain-inhibiting effects were 61.36% and 65.89% in 1st and 2nd phases at 10 mg/kg i.p., respectively. Administration of atropine (non-selective cholinergic receptor antagonist) significantly (p < 0.05) antagonized the antihyperalgesic effect of the compound, while glibenclamide and naloxone did not alter the induced antinociceptive effect and thus, antinociceptive activity of the compound is mediated, at least in part, through cholinergic system antagonism; independent of calcium channel and opioidergic receptor participation. Furthermore, docking studies underlined strong COX-2 inhibitory activity of the compound. RESULT: Our data concluded that overall analgesic activity of the compound seems to involve COX-2 inhibition and activation of cholinergic receptors. However, further detailed studies are required in this direction to confirm the analgesic effect of the compound for its possible clinical utility.
[Mh] Termos MeSH primário: Analgésicos/farmacologia
Dryopteris/química
Quempferóis/farmacologia
[Mh] Termos MeSH secundário: Animais
Masculino
Camundongos
Camundongos Endogâmicos BALB C
Simulação de Acoplamento Molecular
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Analgesics); 0 (Kaempferols)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170209
[Lr] Data última revisão:
170209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160826
[St] Status:MEDLINE


  6 / 84 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:27294155
[Au] Autor:Chi C; Giri SS; Jun JW; Kim HJ; Yun S; Kim SG; Park SC
[Ad] Endereço:Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 151742, Republic of Korea.
[Ti] Título:Immunomodulatory Effects of a Bioactive Compound Isolated from Dryopteris crassirhizoma on the Grass Carp Ctenopharyngodon idella.
[So] Source:J Immunol Res;2016:3068913, 2016.
[Is] ISSN:2314-7156
[Cp] País de publicação:Egypt
[La] Idioma:eng
[Ab] Resumo:In the present study, we investigated effects of compound kaempferol 3-a-L-(4-O-acetyl)rhamnopyranoside-7-a-L-rhamnopyranoside (SA) isolated from Dryopteris crassirhizoma during immune-related gene expression in Ctenopharyngodon idella head kidney macrophages (CIHKM). The expression of immune-related genes (IL-1ß, TNF-α, MyD88, and Mx1) were investigated using real-time PCR at 2 h, 8 h, 12 h, and 24 h after incubation with 1, 10, and 50 µg mL(-1) of SA. Furthermore, fish were injected intraperitoneally with 100 µL of SA, and immune parameters such as lysozyme activity, complement C3, SOD, phagocytic activity, and IgM level were examined at 1, 2, and 3 weeks after injection. The differential expression of cytokines was observed after exposure to SA. IL-1ß genes displayed significant expression at 2 and 8 h after exposure to 1-10 µg mL(-1) of SA. SA also induced gene expression of cytokines such as MyD88, Mx1, and TNF-α. Furthermore, enhanced immune parameters in grass carp confirmed the immunomodulatory activity of SA. Interestingly, this compound has no toxic effect on CIHKM cells as tested by MTT assay. In addition, fish immunised with 10 µg mL(-1) of SA exhibited maximum resistance against Aeromonas hydrophila infection. These results suggest that SA has the potential to stimulate immune responses in grass carp.
[Mh] Termos MeSH primário: Produtos Biológicos/farmacologia
Carpas/imunologia
Dryopteris/química
Imunomodulação/efeitos dos fármacos
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Aeromonas hydrophila
Animais
Produtos Biológicos/isolamento & purificação
Carpas/genética
Carpas/microbiologia
Resistência à Doença/imunologia
Doenças dos Peixes/imunologia
Doenças dos Peixes/microbiologia
Expressão Gênica
Extratos Vegetais/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biological Products); 0 (Plant Extracts)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170306
[Lr] Data última revisão:
170306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160614
[St] Status:MEDLINE
[do] DOI:10.1155/2016/3068913


  7 / 84 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27243922
[Au] Autor:Su Y; Wan D; Song W
[Ad] Endereço:aDepartment of Orthopaedics, Shanghai Jiaotong University Affiliated Sixth People's Hospital bDepartment of Orthopaedics, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, People's Republic of China.
[Ti] Título:Dryofragin inhibits the migration and invasion of human osteosarcoma U2OS cells by suppressing MMP-2/9 and elevating TIMP-1/2 through PI3K/AKT and p38 MAPK signaling pathways.
[So] Source:Anticancer Drugs;27(7):660-8, 2016 Aug.
[Is] ISSN:1473-5741
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Dryofragin, a phloroglucinol derivative extracted from Dryopteris fragrans (L.) Schott, was found to inhibit proliferation and induce apoptosis of tumor cells. However, the mechanism involved in the suppression of cancer cell metastasis by dryofragin remains unclear. Our study investigated the mechanisms for the antitumor properties of dryofragin on the migration and invasion of human osteosarcoma U2OS cells. Dryofragin suppressed the migration and invasive ability of U2OS cells, and it decreased the expression of MMP-2 and MMP-9 and elevated the expression of TIMP-1 and TIMP-2. Western blotting assays indicated that dryofragin was effective in suppressing the phosphorylation of phosphatidylinositide-3 kinase (PI3K), Akt, and p38 MAPK. These results suggest that dryofragin inhibited U2OS cell migration and invasion by reducing the expression of MMP-2 and MMP-9 and elevating the expression of TIMP-1 and TIMP-2 through the PI3K/AKT and p38 MAPK signaling pathways. Above all, we conclude that dryofragin represents an anti-invasive agent and may potentially be applicable in osteosarcoma therapy.
[Mh] Termos MeSH primário: Neoplasias Ósseas/tratamento farmacológico
Inibidores de Metaloproteinases de Matriz/farmacologia
Osteossarcoma/tratamento farmacológico
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Neoplasias Ósseas/enzimologia
Neoplasias Ósseas/metabolismo
Neoplasias Ósseas/patologia
Linhagem Celular Tumoral
Movimento Celular/efeitos dos fármacos
Dryopteris/química
Seres Humanos
Sistema de Sinalização das MAP Quinases/efeitos dos fármacos
Metaloproteinase 2 da Matriz/metabolismo
Metaloproteinase 9 da Matriz/metabolismo
Osteossarcoma/enzimologia
Osteossarcoma/metabolismo
Osteossarcoma/patologia
Inibidor Tecidual de Metaloproteinase-1/metabolismo
Inibidor Tecidual de Metaloproteinase-2/metabolismo
Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Matrix Metalloproteinase Inhibitors); 0 (Plant Extracts); 0 (TIMP1 protein, human); 0 (TIMP2 protein, human); 0 (Tissue Inhibitor of Metalloproteinase-1); 127497-59-0 (Tissue Inhibitor of Metalloproteinase-2); EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases); EC 3.4.24.24 (MMP2 protein, human); EC 3.4.24.24 (Matrix Metalloproteinase 2); EC 3.4.24.35 (MMP9 protein, human); EC 3.4.24.35 (Matrix Metalloproteinase 9)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171020
[Lr] Data última revisão:
171020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160601
[St] Status:MEDLINE
[do] DOI:10.1097/CAD.0000000000000381


  8 / 84 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26700189
[Au] Autor:Peng B; Bai RF; Li P; Han XY; Wang H; Zhu CC; Zeng ZP; Chai XY
[Ad] Endereço:a Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University, Beijing Institute of Traditional Chinese Medicine , Beijing 100010 , China.
[Ti] Título:Two new glycosides from Dryopteris fragrans with anti-inflammatory activities.
[So] Source:J Asian Nat Prod Res;18(1):59-64, 2016.
[Is] ISSN:1477-2213
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Phytochemical investigation on the aqueous extract from Dryopteris fragrans led to the isolation of one new chromone glycoside, frachromone C (1), and one new coumarin glycoside, dryofracoulin A (2), together with one known undulatoside A (3). Their structures were elucidated by a combination of 1D and 2D NMR, HRMS, and chemical analysis. Compounds 1-3 exhibited inhibition on nitric oxide production in lipopolysaccharide induced RAW 264.7 macrophages with their IC50 values of 45.8, 65.8, and 49.8 µM, respectively.
[Mh] Termos MeSH primário: Anti-Inflamatórios/isolamento & purificação
Anti-Inflamatórios/farmacologia
Cumarínicos/isolamento & purificação
Cumarínicos/farmacologia
Medicamentos de Ervas Chinesas/isolamento & purificação
Medicamentos de Ervas Chinesas/farmacologia
Dryopteris/química
Glicosídeos/isolamento & purificação
Glicosídeos/farmacologia
[Mh] Termos MeSH secundário: Animais
Anti-Inflamatórios/química
Cromonas
Cumarínicos/química
Medicamentos de Ervas Chinesas/química
Glicosídeos/química
Lipopolissacarídeos/farmacologia
Macrófagos/efeitos dos fármacos
Camundongos
Estrutura Molecular
Óxido Nítrico/biossíntese
Ressonância Magnética Nuclear Biomolecular
Extratos Vegetais/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Chromones); 0 (Coumarins); 0 (Drugs, Chinese Herbal); 0 (Glycosides); 0 (Lipopolysaccharides); 0 (Plant Extracts); 0 (dryofracoulin A); 0 (frachromone C); 31C4KY9ESH (Nitric Oxide)
[Em] Mês de entrada:1607
[Cu] Atualização por classe:160216
[Lr] Data última revisão:
160216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151225
[St] Status:MEDLINE
[do] DOI:10.1080/10286020.2015.1121853


  9 / 84 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26596576
[Au] Autor:Gao C; Guo N; Li N; Peng X; Wang P; Wang W; Luo M; Fu YJ
[Ad] Endereço:College of Basic Medical Science, Peking University Health Science Center, Beijing, 100083, People's Republic of China.
[Ti] Título:Investigation of antibacterial activity of aspidin BB against Propionibacterium acnes.
[So] Source:Arch Dermatol Res;308(2):79-86, 2016 Mar.
[Is] ISSN:1432-069X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:In the present study, antibacterial activity of four kinds of phloroglucinol derivatives extracted from Dryopteris fragrans (L.) Schott against S. aureus, S. epidermidis and P. acnes has been tested. Aspidin BB exerted the strongest antibacterial activity with minimal inhibition concentration (MIC) values ranging from 7.81 to 15.63 µg/mL. The time-kill assay indicated that aspidin BB could kill P. acnes completely at 2 MIC (MBC) within 4 h. By using AFM, we demonstrated extensive cell surface alterations of aspidin BB-treated P. acnes. SDS-PAGE of supernatant proteins and lipid peroxidation results showed that aspidin BB dose-dependently affected membrane permeability of P. acnes. DNA damage and protein degradation of P. acnes were also verified. SDS-PAGE of precipitated proteins revealed possible targets of aspidin BB, i.e., heat shock proteins (26 kDa) and lipase (33 kDa) which could all cause inflammation. Aspidin BB also seriously increased the inhibition rate of lipase activity from 10.20 to 65.20 % to possibly inhibit the inflammation. In conclusions, the effective constituents of D. fragrans (L.) Schott to treat acne might be phloroglucinol derivatives including aspidin BB, aspidin PB, aspidinol and dryofragin. Among this, aspidin BB inhibited the growth of P. acnes by disrupting their membrane, DNA and proteins and finally leaded to the cell death. The obtained data highlighted the potential of using aspidin BB as an alternative treatment for acne vulgaris.
[Mh] Termos MeSH primário: Acne Vulgar/tratamento farmacológico
Antibacterianos/uso terapêutico
Floroglucinol/análogos & derivados
Propionibacterium acnes/efeitos dos fármacos
Staphylococcus aureus/efeitos dos fármacos
[Mh] Termos MeSH secundário: Proteínas de Bactérias/metabolismo
Permeabilidade da Membrana Celular/efeitos dos fármacos
Fragmentação do DNA/efeitos dos fármacos
Dryopteris
Eletroforese em Gel de Poliacrilamida
Seres Humanos
Inflamação/tratamento farmacológico
Lipase/antagonistas & inibidores
Testes de Sensibilidade Microbiana
Microscopia de Força Atômica
Floroglucinol/uso terapêutico
Extratos Vegetais/uso terapêutico
Proteólise/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 0 (Plant Extracts); DHD7FFG6YS (Phloroglucinol); EC 3.1.1.3 (Lipase); N21SD2R570 (aspidin BB)
[Em] Mês de entrada:1611
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151125
[St] Status:MEDLINE
[do] DOI:10.1007/s00403-015-1603-x


  10 / 84 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26476395
[Au] Autor:Ekrt L; Koutecký P
[Ad] Endereço:Department of Botany, Faculty of Science, University of South Bohemia, Branisovská 1760, CZ-370 05 Ceské Budejovice, Czech Republic libor.ekrt@gmail.com.
[Ti] Título:Between sexual and apomictic: unexpectedly variable sporogenesis and production of viable polyhaploids in the pentaploid fern of the Dryopteris affinis agg. (Dryopteridaceae).
[So] Source:Ann Bot;117(1):97-106, 2016 Jan.
[Is] ISSN:1095-8290
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND AND AIMS: In ferns, apomixis is an important mode of asexual reproduction. Although the mechanisms of fern reproduction have been studied thoroughly, most previous work has focused on cases in which ferns reproduce either exclusively sexually or exclusively asexually. Reproduction of ferns with potentially mixed systems and inheritance of apomixis remains largely unknown. This study addresses reproduction of the pentaploid Dryopteris × critica, a hybrid of triploid apomictic D. borreri and tetraploid sexual D. filix-mas. METHODS: Spore size, abortion percentage and number of spores per sporangium were examined in pentaploid plants of D. × critica grown in an experimental garden. The sporangial content of leaf segments was cultivated on an agar medium, and DNA ploidy levels were estimated by DAPI flow cytometry in 259 gametophytes or sporophytes arising from the F2 generation of the pentaploid hybrid. KEY RESULTS: The hybrid is partly fertile (89-94% of aborted spores) and shows unstable sporogenesis with sexual and apomictic reproduction combined. The number of spores per sporangium varied from approx. 31 to 64. Within a single sporangium it was possible to detect formation of either only aborted spores or various mixtures of aborted and well-developed reduced spores and unreduced diplospores. The spores germinated in viable gametophytes with two ploidy levels: pentaploid (5x, from unreduced spores) and half of that (approx. 2·5x, from reduced spores). Moreover, 2-15% of gametophytes (both 2·5x and 5x) formed a viable sporophyte of the same ploidy level due to apogamy. CONCLUSIONS: This study documents the mixed reproductive mode of a hybrid between apomictic and sexual ferns. Both sexual reduced and apomictic unreduced spores can be produced by a single individual, and even within a single sporangium. Both types of spores give rise to viable F2 generation gametophytes and sporophytes.
[Mh] Termos MeSH primário: Apomixia
Dryopteris/fisiologia
Gametogênese Vegetal/fisiologia
Haploidia
Tetraploidia
[Mh] Termos MeSH secundário: Cruzamentos Genéticos
DNA de Plantas/metabolismo
Dryopteris/genética
Citometria de Fluxo
Gametogênese Vegetal/genética
Tamanho do Genoma
Genoma de Planta
Germinação
Esporos/citologia
Esporos/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Plant)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151018
[St] Status:MEDLINE
[do] DOI:10.1093/aob/mcv152



página 1 de 9 ir para página                      
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde