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Pesquisa : B01.650.940.800.575.912.063.750.044 [Categoria DeCS]
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[PMID]:28653913
[Au] Autor:Hamid J; Ahmed D; Waheed A
[Ad] Endereço:Department of Chemistry, Forman Christian College, Lahore, Pakistan.
[Ti] Título:Evaluation of anti-oxidative, antimicrobial and anti-diabetic potential of Adiantum venustum and identification of its phytochemicals through GC-MS.
[So] Source:Pak J Pharm Sci;30(3):705-712, 2017 May.
[Is] ISSN:1011-601X
[Cp] País de publicação:Pakistan
[La] Idioma:eng
[Ab] Resumo:The present study aimed to explore antioxidant, antimicrobial and anti-diabetic properties of the fern Adiantum venustum. The TPC (total phenolic content) of methanolic extract of the plant was 247.95±0.0007µg of Gallic acid equivalents per gram of dried extract (mg GAE/g). The highest TPC was in n-butanolic fraction, which was 981.45±0.1562mg GAE/g. Hexane fraction showed lowest TPC (256.95±0.0420mg GAE/g). Ethyl acetate fraction exhibited highest total flavonoid content (TFC), i.e., 62.0±0.050mg of Rutin equivalents per gram of sample. DPPH (1,1-diphenyl-2- picryl hydrazyl) radical scavenging activity of the plant was significant. n-Butanolic fraction was most potent with IC being 1.06mg/mL. The IC of methanolic extract was 1.50mg/mL, that of aqueous fraction was 2.51 mg/mL, and that of chloroform fraction was 2.65mg/mL. Antibacterial potential of the fern was tested against two Gram-positive bacterial strains, Pseudomonas aeruginosa and Escherichia coli and two Gram-negative bacterial strains, Bacillus subtilis and Staphylococcus aureus. n-Butanolic fraction showed highest zone of inhibition (ZOI, 25.13±1.237 mm) against P. aeruginosa. Ethyl acetate fraction was most active (ZOI, 15.75±1.060 mm) against S. aureus. Against E. coli, aqueous and n-butanolic fractions were most active (ZOI, 14.75±0.353 and 14.50±0.707mm, respectively). Minimum inhibitory concentration (MIC) of methanolic extract against B. subtilis was as low as 1.98mg/mL. The hexane fraction was least toxic against all the fractions. The plant displayed significant alpha-amylase inhibitory activity. Chloroform fraction was most effective with lowest IC (1.10mg/mL) followed by ethyl acetate fraction (1.92mg/mL). A. venustum is rich in phenolics and has substantial antioxidant, antimicrobial and alpha-amylase inhibitory potential.
[Mh] Termos MeSH primário: Adiantum/química
Anti-Infecciosos/farmacologia
Antioxidantes/farmacologia
Hipoglicemiantes/farmacologia
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Cromatografia Gasosa-Espectrometria de Massas
Testes de Sensibilidade Microbiana
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Antioxidants); 0 (Hypoglycemic Agents); 0 (Plant Extracts)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170907
[Lr] Data última revisão:
170907
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170628
[St] Status:MEDLINE


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[PMID]:28379966
[Au] Autor:Wang AH; Wang FG; Zhang WW; Ma XD; Li XW; Yi QF; Li DL; Duan L; Yan YH; Xing FW
[Ad] Endereço:Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China.
[Ti] Título:Revision of series Gravesiana (Adiantum L.) based on morphological characteristics, spores and phylogenetic analyses.
[So] Source:PLoS One;12(4):e0172729, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Since the adoption of some ambiguous and quantitative characters in Flora Republicae Popularis Sinicae 3(1), species identifications of the series Gravesiana have been in disarray, requiring clarification. Two hundred and fifty-nine individuals from 47 different populations were collected for the estimation of morphological characters and phylogenetic analyses. Spores of 26 populations were observed through scanning electron microscope. Our results were different from those of previous research: (1) six identifiable species, rather than five species observed previously, were confirmed in the series Gravesiana, they are A. gravesii, A. juxtapositum, A. mariesii, A. dentatum, A. longzhouensis and A. obovatum, of which the latter three are newly recognized species. (2) Thirteen characters were measured and estimated through the program Mesquite v. 2.71. The character whether the pinna stalks were 1/3-1/2 times longer than the pinna was used to distinguish A. gravesii and A. lianxianense previously and was found to be unreliable here, whereas such characters as the height of the plant (H), pinna aligned forms (FP), number of pinna (NP), pinna margin (M), number of veins flabellate at base (NV), sori number and shape per pinna (NSS), pinna texture (T), and powder-covered or not on the abaxial surface of the pinna (P) are estimated to be stable and reliable characters useful for identification. Descriptions of new species and their retrieve keys are also listed. (3) Surface ornamentations and spore sizes are helpful for us to distinguish species in series Gravesiana.
[Mh] Termos MeSH primário: Adiantum/classificação
Esporos/classificação
[Mh] Termos MeSH secundário: Microscopia Eletrônica de Varredura/métodos
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170830
[Lr] Data última revisão:
170830
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170406
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0172729


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[PMID]:27663206
[Au] Autor:Kasabri V; Al-Hallaq EK; Bustanji YK; Abdul-Razzak KK; Abaza IF; Afifi FU
[Ad] Endereço:a School of Pharmacy , The University of Jordan , Amman , Jordan.
[Ti] Título:Antiobesity and antihyperglycaemic effects of Adiantum capillus-veneris extracts: in vitro and in vivo evaluations.
[So] Source:Pharm Biol;55(1):164-172, 2017 Dec.
[Is] ISSN:1744-5116
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:CONTEXT: Adiantum capillus-veneris L. (Adiantaceae) hypocholesterolemic activity is therapeutically praised. OBJECTIVES: Pharmacological modulation of pancreatic triacylglycerol lipase (PL) and α-amylase/α-glucosidase by A. capillus-veneris are evaluated. MATERIALS AND METHODS: Using positive controls (acarbose, orlistat, guar gum, atorvastatin, glipizide and metformin) as appropriate, crude aqueous extracts (AEs) of A. capillus-veneris aerial parts were tested via a combination of in vitro enzymatic (0.24-100 mg/mL), acute in vivo carbohydrate tolerance tests (125, 250 or 500 mg/kg body weight [b.wt]) and chronic in vivo studies (500 mg/kg b.wt) in high cholesterol diet (HCD) fed Wistar rats. RESULTS: Like acarbose, A. capillus-veneris as well as chlorogenic acid, with respective IC values (mg/mL) of 0.8 ± 0.0 and 0.2 ± 0.0, were identified as in vitro potent dual inhibitors of α-amylase/α-glucosidase. Unlike guar gum, A. capillus-veneris had no glucose diffusion hindrance capacity. Equivalent to orlistat, A. capillus-veneris and its phytoconstituents inhibited PL in vitro with an ascending order of PL- IC values (µg/mL): ferulic acid; 0.48 ± 0.06 < ellagic acid; 13.53 ± 1.83 < chlorogenic acid; 38.4 ± 2.8 < A. capillus-veneris; 1600 ± 100. Incomparable to acarbose or metformin and glipizide, A. capillus-veneris (125, 250 and 500 mg/kg b.wt) lacked antihyperglycaemic efficacies in acute starch- or glucose-evoked postprandial hyperglycaemia increments in normoglycaemic overnight fasting rats. Superior to atorvastatin; A. capillus-veneris exerted significant antiobesity (p < 0.001) with marked triacylglycerol-reducing capacities (p < 0.001) in comparison to rats fed with HCD for 10 weeks. DISCUSSION AND CONCLUSION: A. capillus-veneris, modulating pancreatic digestive enzymes, may be advocated as a combinatorial diabesity prevention/phytotherapy agent.
[Mh] Termos MeSH primário: Adiantum/química
Fármacos Antiobesidade/farmacologia
Inibidores de Glicosídeo Hidrolases/farmacologia
Hipoglicemiantes/farmacologia
Lipase/antagonistas & inibidores
Obesidade/prevenção & controle
Pâncreas/efeitos dos fármacos
Extratos Vegetais/farmacologia
alfa-Amilases/antagonistas & inibidores
[Mh] Termos MeSH secundário: Animais
Fármacos Antiobesidade/isolamento & purificação
Biomarcadores/sangue
Glicemia/efeitos dos fármacos
Glicemia/metabolismo
Dieta Hiperlipídica
Modelos Animais de Doenças
Relação Dose-Resposta a Droga
Teste de Tolerância a Glucose
Inibidores de Glicosídeo Hidrolases/isolamento & purificação
Hipoglicemiantes/isolamento & purificação
Lipase/metabolismo
Obesidade/enzimologia
Obesidade/etiologia
Obesidade/fisiopatologia
Pâncreas/enzimologia
Fitoterapia
Componentes Aéreos da Planta
Extratos Vegetais/isolamento & purificação
Plantas Medicinais
Ratos Wistar
Fatores de Tempo
Triglicerídeos/sangue
Ganho de Peso/efeitos dos fármacos
alfa-Amilases/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Obesity Agents); 0 (Biomarkers); 0 (Blood Glucose); 0 (Glycoside Hydrolase Inhibitors); 0 (Hypoglycemic Agents); 0 (Plant Extracts); 0 (Triglycerides); EC 3.1.1.3 (Lipase); EC 3.2.1.1 (alpha-Amylases)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170309
[Lr] Data última revisão:
170309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160925
[St] Status:MEDLINE


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[PMID]:27716154
[Au] Autor:Galehdari H; Negahdari S; Kesmati M; Rezaie A; Shariati G
[Ad] Endereço:Toxicology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. galehdari187@yahoo.com.
[Ti] Título:Effect of the herbal mixture composed of Aloe Vera, Henna, Adiantum capillus-veneris, and Myrrha on wound healing in streptozotocin-induced diabetic rats.
[So] Source:BMC Complement Altern Med;16(1):386, 2016 Oct 06.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Wound healing is often impaired in diabetic animals and humans. Matrix metalloproteases act as pro-inflammatory agents in physiological wound healing pathways by stimulating cytokines including the interleukins, IL6, IL1A and IL1B, and the tumor necrosis factor and transforming growth factor beta1. Botanicals are traditionally used to assist healing of different types of wounds, because they produce fewer side effects. Our specific aim here was to develop a plant-based recipe supporting effective wound healing in diabetic animals. METHODS: Plant materials from Adiantum capillus-veneris, Commiphora molmol, Aloe Vera, and henna were collected for this study, and oven-dried at 60 °C. The dried leaves and resins were then crumbled into a powder and mixed in equal parts with Vaseline as a preservative. This mixture was used as an ointment on wounds induced in 60 diabetic and non-diabetic rats that were divided into 6 subgroups receiving agent or control treatments. Necrotic tissue surrounding the wound was periodically removed during wound healing. RNA was extracted from the healing region of the wound at days 7, 14 and 21 for cDNA synthesis to monitor changes in Tgfb1, Mmp3, Mmp9, Il6 and Tnf α expression using real-time PCR. RESULTS: The expression of the Mmp3, the Tnf α, and the Tgfb1 genes from wound tissue were significantly different (p < 0.05) between diabetic and non-diabetic (control) rats treated with the herbal mixture after 14 and 21 days. There was no significant difference (p > 0.05) of the Mmp9 gene expression in diabetic and non-diabetic rats treated only with Vaseline after 7, 14, and 21 days. But, the expression of the Mmp9 gene decreased significantly (p < 0.05) in diabetic rats after 14 days in comparison to non-diabetic rats, when the herbal mixture was added to Vaseline. CONCLUSIONS: Our study presents an herbal treatment that alters the gene expression signature at wounds induced in the rat model for type I diabetes in a manner consistent with accelerated healing, and demonstrates that this herbal treatment might be effective to treat wounds in diabetic patients.
[Mh] Termos MeSH primário: Adiantum/química
Aloe/química
Diabetes Mellitus Experimental/metabolismo
Lawsonia (Planta)/química
Extratos Vegetais/farmacologia
Cicatrização/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Expressão Gênica/efeitos dos fármacos
Masculino
Metaloproteinases da Matriz/metabolismo
Extratos Vegetais/química
Ratos
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Extracts); EC 3.4.24.- (Matrix Metalloproteinases)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161008
[St] Status:MEDLINE


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[PMID]:27603700
[Au] Autor:Wang FH; Lu JM; Wen J; Ebihara A; Li DZ
[Ad] Endereço:Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan, China.
[Ti] Título:Applying DNA Barcodes to Identify Closely Related Species of Ferns: A Case Study of the Chinese Adiantum (Pteridaceae).
[So] Source:PLoS One;11(9):e0160611, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:DNA barcoding is a fast-developing technique to identify species by using short and standard DNA sequences. Universal selection of DNA barcodes in ferns remains unresolved. In this study, five plastid regions (rbcL, matK, trnH-psbA, trnL-F and rps4-trnS) and eight nuclear regions (ITS, pgiC, gapC, LEAFY, ITS2, IBR3_2, DET1, and SQD1_1) were screened and evaluated in the fern genus Adiantum from China and neighboring areas. Due to low primer universality (matK) and/or the existence of multiple copies (ITS), the commonly used barcodes matK and ITS were not appropriate for Adiantum. The PCR amplification rate was extremely low in all nuclear genes except for IBR3_2. rbcL had the highest PCR amplification rate (94.33%) and sequencing success rate (90.78%), while trnH-psbA had the highest species identification rate (75%). With the consideration of discriminatory power, cost-efficiency and effort, the two-barcode combination of rbcL+ trnH-psbA seems to be the best choice for barcoding Adiantum, and perhaps basal polypod ferns in general. The nuclear IBR3_2 showed 100% PCR amplification success rate in Adiantum, however, it seemed that only diploid species could acquire clean sequences without cloning. With cloning, IBR3_2 can successfully distinguish cryptic species and hybrid species from their related species. Because hybridization and allopolyploidy are common in ferns, we argue for including a selected group of nuclear loci as barcodes, especially via the next-generation sequencing, as it is much more efficient to obtain single-copy nuclear loci without the cloning procedure.
[Mh] Termos MeSH primário: Adiantum/genética
Código de Barras de DNA Taxonômico
Gleiquênias/genética
[Mh] Termos MeSH secundário: Adiantum/classificação
China
DNA de Plantas/genética
DNA Espaçador Ribossômico/genética
Gleiquênias/classificação
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Plant); 0 (DNA, Ribosomal Spacer)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170802
[Lr] Data última revisão:
170802
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160908
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0160611


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[PMID]:27216814
[Au] Autor:Hou CJ; Yang CH
[Ad] Endereço:Institute of Biotechnology, National Chung Hsing University, Taichung, 40227, Taiwan, ROC.
[Ti] Título:Comparative analysis of the pteridophyte Adiantum MFT ortholog reveals the specificity of combined FT/MFT C and N terminal interaction with FD for the regulation of the downstream gene AP1.
[So] Source:Plant Mol Biol;91(4-5):563-79, 2016 Jul.
[Is] ISSN:1573-5028
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:To study the evolution of phosphatidylethanolamine-binding protein (PEBP) gene families in non-flowering plants, we performed a functional analysis of the PEBP gene AcMFT of the MFT clade in the pteridophyte Adiantum capillus-veneris. The expression of AcMFT was regulated by photoperiod similar to that for FT under both long day and short day conditions. Ectopic expression of AcMFT in Arabidopsis promotes the floral transition and partially complements the late flowering defect in transgenic Arabidopsis ft-1 mutants, suggesting that AcMFT functions similarly to FT in flowering plants. Interestingly, a similar partial compensation of the ft-1 late flowering phenotype was observed in Arabidopsis ectopically expressing only exon 4 of the C terminus of AcMFT and FT. This result indicated that the fourth exon of AcMFT and FT plays a similar and important role in promoting flowering. Further analysis indicated that exons 1-3 in the N terminus specifically enhanced the function of FT exon 4 in controlling flowering in Arabidopsis. Protein pull-down assays indicated that Arabidopsis FD proteins interact with full-length FT and AcMFT, as well as peptides encoded by 1-3 exon fragments or the 4th exon alone. Furthermore, similar FRET efficiencies for FT-FD and AcMFT-FD heterodimer in nucleus were observed. These results indicated that FD could form the similar complex with FT and AcMFT. Further analysis indicated that the expression of AP1, a gene downstream of FT, was up-regulated more strongly by FT than AcMFT in transgenic Arabidopsis. Our results revealed that AcMFT from a non-flowering plant could interact with FD to regulate the floral transition and that this function was reduced due to the weakened ability of AcMFT-FD to activate the downstream gene AP1.
[Mh] Termos MeSH primário: Adiantum/metabolismo
Proteínas de Arabidopsis/metabolismo
Arabidopsis/metabolismo
Regulação da Expressão Gênica de Plantas
Proteínas de Domínio MADS/metabolismo
Proteínas de Plantas/metabolismo
Homologia de Sequência de Aminoácidos
Fatores de Transcrição/metabolismo
[Mh] Termos MeSH secundário: Adiantum/genética
Sequência de Aminoácidos
DNA Complementar/genética
DNA Complementar/isolamento & purificação
Éxons/genética
Flores/genética
Flores/fisiologia
Genes de Plantas
Mutação/genética
Fenótipo
Filogenia
Proteínas de Plantas/química
Proteínas de Plantas/genética
Plantas Geneticamente Modificadas
Ligação Proteica
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (AP1 protein, Arabidopsis); 0 (Arabidopsis Proteins); 0 (DNA, Complementary); 0 (FD protein, Arabidopsis); 0 (MADS Domain Proteins); 0 (Plant Proteins); 0 (Transcription Factors)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160525
[St] Status:MEDLINE
[do] DOI:10.1007/s11103-016-0489-0


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[PMID]:26936479
[Au] Autor:Yousaf B; Amina; Liu G; Wang R; Qadir A; Ali MU; Kanwal Q; Munir B; Asmatullah; Abbas Z
[Ad] Endereço:Chinese Academy of Science (CAS) Key Laboratory of Crust-Mantle Materials and the Environments, School of Earth and Space Sciences, University of Science and Technology of China, Hefei, 230026, People's Republic of China.
[Ti] Título:Bisphenol A exposure and healing effects of Adiantum capillus-veneris L. plant extract (APE) in bisphenol A-induced reproductive toxicity in albino rats.
[So] Source:Environ Sci Pollut Res Int;23(12):11645-57, 2016 Jun.
[Is] ISSN:1614-7499
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The current study presents the bisphenol A exposure and the ameliorative effects of Adiantum capillus-veneris on testicular toxicity induced by bisphenol A. Adult male albino rats were divided into five groups of five animals each: A (control), B (vehicle control), C (toxic), D (protective), and E (ameliorative) were served distilled water, olive oil, bisphenol A (BPA) at 100 mg/kg body weight, A. capillus-veneris plant extract at 25 mg/kg body weight, and BPA + A. capillus-veneris, respectively. All of the doses were administered orally for 15 days, and the rats were then sacrificed. Blood samples for the testosterone assay and both testes were collected for histological examination. The body weight, paired testes weight, relative tissue weight index, Johnsen scoring of tubules, and level of serum testosterone decreased in BPA-treated rats. Similarly, histological examination of the testes in BPA-treated animals revealed a lower number of Leydig cells, an irregular basement membrane, sloughing of germinal layers, vacuolization, a lower number of spermatocytes, and debris in the lumen. However, co-administration of A. capillus-veneris with BPA increased the total antioxidative capacity (330.82 ± 22.46 µmol/mg protein) of the testes and restored the serum testosterone level (1.70 ng/ml); histological features showed restoration in the stages of spermatogenesis. Conclusively, A. capillus-veneris plant extract overcomes the estrogenic effects of BPA on the reproductive system of rats and protects rats' testes against BPA-induced injury/damage via an antioxidative mechanism that appears to be conciliated.
[Mh] Termos MeSH primário: Adiantum
Antioxidantes/uso terapêutico
Compostos Benzidrílicos/toxicidade
Estrogênios não Esteroides/toxicidade
Fenóis/toxicidade
Extratos Vegetais/uso terapêutico
Reprodução/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Antioxidantes/farmacologia
Disruptores Endócrinos/toxicidade
Masculino
Tamanho do Órgão/efeitos dos fármacos
Extratos Vegetais/farmacologia
Ratos
Testículo/efeitos dos fármacos
Testículo/patologia
Testosterona/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Benzhydryl Compounds); 0 (Endocrine Disruptors); 0 (Estrogens, Non-Steroidal); 0 (Phenols); 0 (Plant Extracts); 3XMK78S47O (Testosterone); MLT3645I99 (bisphenol A)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160304
[St] Status:MEDLINE
[do] DOI:10.1007/s11356-016-6330-0


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[PMID]:26838902
[Au] Autor:Kalita S; Kandimalla R; Sharma KK; Kataki AC; Deka M; Kotoky J
[Ad] Endereço:Drug Discovery Lab, Life Science Division, Institute of Advanced Study in Science and Technology (IASST), Paschim Boragaon, Garchuk, Guwahati 781035, Assam, India.
[Ti] Título:Amoxicillin functionalized gold nanoparticles reverts MRSA resistance.
[So] Source:Mater Sci Eng C Mater Biol Appl;61:720-7, 2016 Apr 01.
[Is] ISSN:1873-0191
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In this study, we have described the biosynthesis of biocompatible gold nanoparticles (GNPs) from aqueous extract of the aerial parts of a pteridophyte, "Adiantum philippense" by microwave irradiation and its surface functionalization with broad spectrum beta lactam antibiotic, amoxicillin (Amox). The functionalization of amoxicillin on GNPs (GNP-Amox) was carried out via electrostatic interaction of protonated amino group and thioether moiety mediated attractive forces. The synthesized GNPs and GNP-Amox were physicochemically characterized. UV-Vis spectroscopy, Zeta potential, XRD, FTIR and SERS (surface enhanced raman spectra) results confirmed the loading of Amox into GNPs. Loading of Amox to GNPs reduce amoxicillin cytotoxicity, whereas GNPs were found to be nontoxic to mouse fibroblast cell line (L929) as evident from MTT and acridine orange/ethidium bromide (AO/EtBr) live/dead cell assays. The GNP-Amox conjugates demonstrated enhanced broad-spectrum bactericidal activity against both Gram-positive and Gram-negative bacteria. Furthermore, in-vitro and in-vivo assays of GNP-Amox revealed potent anti-MRSA activity and improved the survival rate. This indicates the subversion of antibiotic resistance mechanism by overcoming the effect of high levels of ß-lactamase produced by methicillin resistant Staphylococcus aureus (MRSA). Taken together, this study demonstrates the positive attributes from GNP-Amox conjugates as a promising antibacterial therapeutic agent against MRSA as well as other pathogens.
[Mh] Termos MeSH primário: Amoxicilina/química
Antibacterianos/química
Portadores de Fármacos/química
Ouro/química
Nanopartículas Metálicas/química
[Mh] Termos MeSH secundário: Adiantum/química
Adiantum/metabolismo
Amoxicilina/administração & dosagem
Amoxicilina/toxicidade
Animais
Antibacterianos/administração & dosagem
Antibacterianos/farmacologia
Linhagem Celular
Sobrevivência Celular/efeitos dos fármacos
Modelos Animais de Doenças
Ouro/análise
Bactérias Gram-Negativas/efeitos dos fármacos
Bactérias Gram-Positivas/efeitos dos fármacos
Química Verde
Rim/química
Rim/metabolismo
Fígado/química
Fígado/metabolismo
Nanopartículas Metálicas/ultraestrutura
Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos
Staphylococcus aureus Resistente à Meticilina/patogenicidade
Camundongos
Testes de Sensibilidade Microbiana
Micro-Ondas
Componentes Aéreos da Planta/química
Componentes Aéreos da Planta/metabolismo
Espectrofotometria Atômica
Infecções Estafilocócicas/tratamento farmacológico
Infecções Estafilocócicas/veterinária
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Drug Carriers); 7440-57-5 (Gold); 804826J2HU (Amoxicillin)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160204
[St] Status:MEDLINE


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[PMID]:26281979
[Au] Autor:Bonavita S; Regina TM
[Ad] Endereço:Dipartimento di Biologia, Ecologia e Scienze della Terra (DiBEST), Università della Calabria, via Ponte P. Bucci, 87036, Arcavacata di Rende, Cosenza, Italy.
[Ti] Título:The evolutionary conservation of rps3 introns and rps19-rps3-rpl16 gene cluster in Adiantum capillus-veneris mitochondria.
[So] Source:Curr Genet;62(1):173-84, 2016 Feb.
[Is] ISSN:1432-0983
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Ferns are a large and evolutionarily critical group of vascular land plants for which quite limited mitochondrial gene content and genome organization data are, currently, available. This study reports that the gene for the ribosomal protein S3 (rps3) is preserved and physically clustered to an upstream rps19 and a downstream overlapping rpl16 locus in the mitochondrial DNA of the true fern Adiantum capillus-veneris L. Sequence analysis also revealed that the rps3 gene is interrupted by two cis-splicing group II introns, like the counterpart in lycopod and gymnosperm representatives. A preliminary polymerase chain reaction (PCR) survey confirmed a scattered distribution pattern of both the rps3 introns also in other fern lineages. Northern blot and reverse transcription (RT)-PCR analyses demonstrated that the three ribosomal protein genes are co-transcribed as a polycistronic mRNA and modified by RNA editing. Particularly, the U-to-C type editing amends numerous genomic stop codons in the A. capillus-veneris rps19, rps3 and rpl16 sequences, thus, assuring the synthesis of complete and functional polypeptides. Collectively, the findings from this study further expand our knowledge of the mitochondrial rps3 architecture and evolution, also, bridging the significant molecular data gaps across the so far underrepresented ferns and all land plants.
[Mh] Termos MeSH primário: Adiantum/genética
Evolução Molecular
Genes Mitocondriais
Íntrons
Família Multigênica
Proteínas Ribossômicas/genética
[Mh] Termos MeSH secundário: Sequência de Bases
Regulação da Expressão Gênica de Plantas
Genoma Mitocondrial
Dados de Sequência Molecular
Filogenia
Proteínas Ribossômicas/química
Alinhamento de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Ribosomal Proteins); 0 (ribosomal protein S19); 0 (ribosomal protein S3)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150819
[St] Status:MEDLINE
[do] DOI:10.1007/s00294-015-0512-z


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[PMID]:26444002
[Au] Autor:Poppinga S; Haushahn T; Warnke M; Masselter T; Speck T
[Ad] Endereço:Plant Biomechanics Group, University of Freiburg, Botanic Garden, Faculty of Biology, Freiburg im Breisgau, Germany; Freiburg Materials Research Center (FMF), University of Freiburg, Freiburg im Breisgau, Germany.
[Ti] Título:Sporangium Exposure and Spore Release in the Peruvian Maidenhair Fern (Adiantum peruvianum, Pteridaceae).
[So] Source:PLoS One;10(10):e0138495, 2015.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We investigated the different processes involved in spore liberation in the polypod fern Adiantum peruvianum (Pteridaceae). Sporangia are being produced on the undersides of so-called false indusia, which are situated at the abaxial surface of the pinnule margins, and become exposed by a desiccation-induced movement of these pinnule flaps. The complex folding kinematics and functional morphology of false indusia are being described, and we discuss scenarios of movement initiation and passive hydraulic actuation of these structures. High-speed cinematography allowed for analyses of fast sporangium motion and for tracking ejected spores. Separation and liberation of spores from the sporangia are induced by relaxation of the annulus (the 'throwing arm' of the sporangium catapult) and conservation of momentum generated during this process, which leads to sporangium bouncing. The ultra-lightweight spores travel through air with a maximum velocity of ~5 m s(-1), and a launch acceleration of ~6300 g is measured. In some cases, the whole sporangium, or parts of it, together with contained spores break away from the false indusium and are shed as a whole. Also, spores can stick together and form spore clumps. Both findings are discussed in the context of wind dispersal.
[Mh] Termos MeSH primário: Adiantum/fisiologia
Gleiquênias/fisiologia
Pteridaceae/fisiologia
Esporângios/fisiologia
Esporos/fisiologia
[Mh] Termos MeSH secundário: Movimento/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1606
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151008
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0138495



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