Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.075.144 [Categoria DeCS]
Referências encontradas : 296 [refinar]
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[PMID]:27771535
[Au] Autor:Sim Y; Park G; Eo H; Huh E; Gu PS; Hong SP; Pak YK; Oh MS
[Ad] Endereço:Department of Life and Nanopharmaceutical Sciences, Graduate School, Kyung Hee University, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea.
[Ti] Título:Protective effects of a herbal extract combination of Bupleurum falcatum, Paeonia suffruticosa, and Angelica dahurica against MPTP-induced neurotoxicity via regulation of nuclear receptor-related 1 protein.
[So] Source:Neuroscience;340:166-175, 2017 Jan 06.
[Is] ISSN:1873-7544
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Parkinson's disease (PD) is one of the progressive neurodegenerative diseases of whose condition is characterized by dopaminergic neuronal cell loss and dysfunction in the substantia nigra pars compacta (SNpc) and the striatum. Recent studies have demonstrated that the nuclear receptor-related 1 protein (Nurr1) is critical of dopaminergic phenotype induction in mesencephalic dopaminergic neurons. Further, Nurr1 engages in synthesizing and storing dopamine through regulating levels of tyrosine hydroxylase (TH), dopamine transporter (DAT) and vesicular monoamine transporter 2 (VMAT2). The aim of this study was to investigate the protective effects of a herbal extract combination, consisting of Bupleurum falcatum, Paeonia suffruticosa, and Angelica dahurica (MABH), on 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced PD-like symptoms and to elucidate possible mechanisms of action focusing on Nurr1. In a subacute mouse model of MPTP-induced PD, MABH treatment resulted in recovery from movement impairments. MABH prevented dopamine depletion and protected against dopaminergic neuronal degradation induced by MPTP. Additionally, MABH increased Nurr1 expression in the SNpc of mice. To evaluate the effects of MABH on Nurr1 expression, we measured the protein levels of Nurr1 and its regulating factors using Western blot analysis in PC12 cells. MABH treatment induced the phosphorylation of extracellular signal-regulated kinase protein via increasing the protein expression levels of Nurr1 and ultimately the levels of TH, VMAT2, and DAT. These results indicate that MABH has protective effects on dopaminergic neurons in a mouse model of PD by regulating Nurr1.
[Mh] Termos MeSH primário: Angelica
Bupleurum
Intoxicação por MPTP/tratamento farmacológico
Fármacos Neuroprotetores/farmacologia
Paeonia
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Animais
Dopamina/metabolismo
Expressão Gênica/efeitos dos fármacos
Intoxicação por MPTP/metabolismo
Intoxicação por MPTP/patologia
Masculino
Camundongos Endogâmicos C57BL
Neurônios/efeitos dos fármacos
Neurônios/metabolismo
Neurônios/patologia
Membro 2 do Grupo A da Subfamília 4 de Receptores Nucleares/metabolismo
Células PC12
Parte Compacta da Substância Negra/efeitos dos fármacos
Parte Compacta da Substância Negra/metabolismo
Parte Compacta da Substância Negra/patologia
Fitoterapia
Ratos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Neuroprotective Agents); 0 (Nr4a2 protein, mouse); 0 (Nr4a2 protein, rat); 0 (Nuclear Receptor Subfamily 4, Group A, Member 2); 0 (Plant Extracts); VTD58H1Z2X (Dopamine)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171224
[Lr] Data última revisão:
171224
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


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[PMID]:28628838
[Au] Autor:Lei T; Zhang D; Guo K; Li M; Lv C; Wang J; Jia L; Lu J
[Ad] Endereço:Department of Medicinal Plant, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, PR China.
[Ti] Título:Validated UPLC-MS/MS method for simultaneous quantification of eight saikosaponins in rat plasma: Application to a comparative pharmacokinetic study in depression rats after oral administration of extracts of raw and vinegar-baked Bupleuri Radix.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1060:231-239, 2017 Aug 15.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A sensitive and efficient UPLC-MS/MS method was developed and validated for simultaneous quantification of eight saikosaponins (SSa, SSb , SSb , SSb , SSb , SSc, SSd and SSf) in rat plasma. Furthermore, comparative pharmacokinetic profiles of these sakosaponins were investigated, following oral administration of extracts of raw and vinegar-baked Bupleuri Radix to depression rats. Biosamples were processed with liquid-liquid extraction technique using ethyl acetate. Chromatographic separation was accomplished on Waters BEH C UPLC column. 0.05% formic acid in water and acetonitrile as mobile phase was used at a flow rate of 0.40mL/min. The analytes and internal standard, digoxin, were detected using negative ion electrospray ionization in multiple reaction monitoring mode. The lower limit of quantification was less than 0.62ng/mL for the each analyte. The validation parameters investigated, which were specificity, precision, accuracy, matrix effect, recovery and stability, were well within acceptable limits. Results showed that for some of analytes, AUC and C were significantly different after oral administration of extracts of raw and vinegar-baked Bupleuri Radix. Moreover, the pharmacokinetic study in pathological state could provide more useful information to guide the clinical usage of Bupleuri Radix.
[Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão/métodos
Depressão/metabolismo
Ácido Oleanólico/análogos & derivados
Extratos Vegetais/farmacocinética
Saponinas/sangue
Saponinas/farmacocinética
Espectrometria de Massas em Tandem/métodos
[Mh] Termos MeSH secundário: Ácido Acético
Administração Oral
Animais
Bupleurum
Medicamentos de Ervas Chinesas/administração & dosagem
Medicamentos de Ervas Chinesas/farmacocinética
Modelos Lineares
Masculino
Ácido Oleanólico/sangue
Ácido Oleanólico/química
Ácido Oleanólico/farmacocinética
Extratos Vegetais/administração & dosagem
Ratos
Ratos Sprague-Dawley
Reprodutibilidade dos Testes
Saponinas/química
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bupleurum root extract); 0 (Drugs, Chinese Herbal); 0 (Plant Extracts); 0 (Saponins); 6SMK8R7TGJ (Oleanolic Acid); Q40Q9N063P (Acetic Acid); UR635J3F00 (saikosaponin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170620
[St] Status:MEDLINE


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[PMID]:28606186
[Au] Autor:Zhu L; Liang ZT; Yi T; Ma Y; Zhao ZZ; Guo BL; Zhang JY; Chen HB
[Ad] Endereço:School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, Special Administrative Region, People's Republic of China.
[Ti] Título:Comparison of chemical profiles between the root and aerial parts from three Bupleurum species based on a UHPLC-QTOF-MS metabolomics approach.
[So] Source:BMC Complement Altern Med;17(1):305, 2017 Jun 12.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Bupleuri Radix (Chaihu) represents one of the most successful and widely used herbal medicines in Asia for the treatment of many diseases such as inflammatory disorders and infectious diseases over the past 2000 years. In the Chinese Pharmacopoeia, Chaihu is recorded as the dried roots of Bupleurum chinense DC. and B. scorzonerifolium Willd. (Umbelliferae). However, the widespread demand for the herb has tended to far outstrip the supply. Whether the aerial parts, which account for 70 ~ 85% of the dry weights of Bupleurum species, could be used as an alternative for the root has become an important scientific issue for the sustainable utilization of Bupleurum species. On the other hand, in some areas including the southeast of China as well as in Spain, the aerial parts of Bupleurum species have already been used in the folk medications. Therefore, to clarify whether the root and aerial parts of Bupleurum species are "equivalent" in the types and quantities of chemical constituents which subsequently influence their biological activities and therapeutic effects is of great importance for both the rational and sustainable use of this herb. METHODS: In the present study, the chemical profiles between the root and aerial parts of Bupleurum species from different species and collected from various locations were analyzed and compared by the ultra-high performance liquid chromatography quadrupole/time of flight-mass spectrometry (UHPLC-QTOF-MS). RESULTS: A total of 56 peaks were identified in the root and/or aerial parts from different batches of Bupleurum species, by comparison of references standards or with those reported in the literature. Principal Component Analysis (PCA) was conducted for displaying the differentiating clustering between these two parts. CONCLUSION: The results disclosed the distinct variations between them, which indicated that the aerial parts could not be used as an alternative of root from a chemodiversity perspective. The differentiating markers resulted from the PCA analysis could also be utilized for the differentiation between them. Further validation of their biological differences is anticipated in the future study.
[Mh] Termos MeSH primário: Bupleurum/química
Medicamentos de Ervas Chinesas/química
Componentes Aéreos da Planta/química
[Mh] Termos MeSH secundário: China
Cromatografia Líquida de Alta Pressão
Espectrometria de Massas
Metabolômica
Raízes de Plantas/química
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drugs, Chinese Herbal)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170614
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-017-1816-y


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[PMID]:28423482
[Au] Autor:Zhao Y; Feng LM; Liu LJ; Zhang X; Zhao RZ
[Ad] Endereço:Department of Chinese Medicine Property Team, Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China.
[Ti] Título:Clerosterol from vinegar-baked radix bupleuri modifies drug transport.
[So] Source:Oncotarget;8(13):21351-21361, 2017 Mar 28.
[Is] ISSN:1949-2553
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Vinegar-baked Radix Bupleuri (VBRB) is reportedly used to treat liver cancer when combined with traditional chemotherapy and data show that this combination may modify drug transport. We isolated clerosterol from VBRB and studied its effect on drug transporters in normal or transporter-overexpressing cells. Transporter activity was assayed using cellular substrate concentration and transporter expression with Western blot and RT-qPCR. Clerosterol decreased cisplatin uptake in BRL cells mainly through increasing Mrp2 gene expression. Clerosterol also decreased the uptake of colchicine in HEK 293 cells by increasing both Pgp and Mrp1 activity; in detail, it could increase Pgp protein but had marginal effects on Mrp1 protein and gene expression. Further study showed clerosterol increased OCT2 activity in HEK293-Pgp cells by increasing OCT2 protein and mRNA. Clerosterol could suppress Pgp overexpression but not by regulating protein and gene expression. And clerosterol had marginal effects on Mrp2 and Mrp1 activity in Mrp2- and Mrp1-overexpressing HEK293 cells. Thus, Clerosterol may be an active constituent of VBRB and may work against cancer multidrug resistance by inhibiting Pgp activity.
[Mh] Termos MeSH primário: Membro 1 da Subfamília B de Cassetes de Ligação de ATP/efeitos dos fármacos
Antineoplásicos/farmacologia
Transporte Biológico/efeitos dos fármacos
Resistência a Medicamentos Antineoplásicos/efeitos dos fármacos
Esteroides/farmacologia
[Mh] Termos MeSH secundário: Membro 1 da Subfamília B de Cassetes de Ligação de ATP/biossíntese
Western Blotting
Bupleurum/química
Resistência a Múltiplos Medicamentos/efeitos dos fármacos
Medicamentos de Ervas Chinesas/farmacologia
Células HEK293
Seres Humanos
Proteínas Associadas à Resistência a Múltiplos Medicamentos/biossíntese
Proteínas Associadas à Resistência a Múltiplos Medicamentos/efeitos dos fármacos
Extratos Vegetais/farmacologia
Reação em Cadeia da Polimerase em Tempo Real
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (ATP-Binding Cassette, Sub-Family B, Member 1); 0 (Antineoplastic Agents); 0 (Drugs, Chinese Herbal); 0 (Multidrug Resistance-Associated Proteins); 0 (Plant Extracts); 0 (Steroids); 0 (clerosterol); 4AF605U6JN (multidrug resistance-associated protein 2); Y49M64GZ4Q (multidrug resistance-associated protein 1)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170421
[St] Status:MEDLINE
[do] DOI:10.18632/oncotarget.15212


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[PMID]:28420359
[Au] Autor:Li X; Li X; Lu J; Huang Y; Lv L; Luan Y; Liu R; Sun R
[Ad] Endereço:Department of Medical Pathomorphology, Shandong Academy of Traditional Chinese Medicine, Jinan, 250014, Shandong, China.
[Ti] Título:Saikosaponins induced hepatotoxicity in mice via lipid metabolism dysregulation and oxidative stress: a proteomic study.
[So] Source:BMC Complement Altern Med;17(1):219, 2017 Apr 19.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Radix Bupleuri (RB) has been popularly used for treating many liver diseases such as chronic hepatic inflammation and viral Hepatitis in China. Increasing clinical and experimental evidence indicates the potential hepatotoxicity of RB or prescriptions containing RB. Recently, Saikosaponins (SS) have been identified as major bioactive compounds isolated from RB, which may be also responsible for RB-induced liver injury. METHODS: Serum AST, ALT and LDH levels were determined to evaluate SS-induced liver injury in mice. Serum and liver total triglyceride and cholesterol were used to indicate lipid metabolism homeostasis. Liver ROS, GSH, MDA and iNOS were used to examine the oxidative stress level after SS administration. Western blot was used to detect CYP2E1 expression. A 8-Plex iTRAQ Labeling Coupled with 2D LC - MS/MS technique was applied to analyze the protein expression profiles in livers of mice administered with different doses of SS for different time periods. Gene ontology analysis, cluster and enrichment analysis were employed to elucidate potential mechanism involved. HepG2 cells were used to identify our findings in vitro. RESULTS: SS dose- and time-dependently induced liver injury in mice, indicated by increased serum AST, ALT and LDH levels. According to proteomic analysis, 487 differentially expressed proteins were identified in mice administrated with different dose of SS for different time periods. Altered proteins were enriched in pathways such as lipid metabolism, protein metabolism, macro molecular transportation, cytoskeleton structure and response to stress. SS enhanced CYP2E1 expression in a time and dose dependent manner, and induced oxidative stress both in vivo and in vitro. CONCLUSION: Our results identified hepatotoxicity and established dose-time course-liver toxicity relationship in mice model of SS administration and suggested potential mechanisms, including impaired lipid and protein metabolism and oxidative stress. The current study provides experimental evidence for clinical safe use of RB, and also new insights into understanding the mechanism by which SS and RB induced liver injury.
[Mh] Termos MeSH primário: Bupleurum/química
Medicamentos de Ervas Chinesas/toxicidade
Metabolismo dos Lipídeos/efeitos dos fármacos
Hepatopatias/etiologia
Fígado/efeitos dos fármacos
Ácido Oleanólico/análogos & derivados
Estresse Oxidativo
Saponinas/toxicidade
[Mh] Termos MeSH secundário: Alanina Transaminase/sangue
Animais
Aspartato Aminotransferases/sangue
Colesterol/sangue
Citocromo P-450 CYP2E1/sangue
Medicamentos de Ervas Chinesas/efeitos adversos
Medicamentos de Ervas Chinesas/uso terapêutico
Feminino
Células Hep G2
Seres Humanos
L-Lactato Desidrogenase/sangue
Fígado/enzimologia
Fígado/metabolismo
Hepatopatias/sangue
Hepatopatias/tratamento farmacológico
Hepatopatias/metabolismo
Masculino
Camundongos
Óxido Nítrico Sintase Tipo II/sangue
Ácido Oleanólico/toxicidade
Raízes de Plantas
Proteômica
Triglicerídeos/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drugs, Chinese Herbal); 0 (Saponins); 0 (Triglycerides); 6SMK8R7TGJ (Oleanolic Acid); 97C5T2UQ7J (Cholesterol); EC 1.1.1.27 (L-Lactate Dehydrogenase); EC 1.14.13.- (Cytochrome P-450 CYP2E1); EC 1.14.13.- (cytochrome P-450 2E1, mouse); EC 1.14.13.39 (Nitric Oxide Synthase Type II); EC 2.6.1.1 (Aspartate Aminotransferases); EC 2.6.1.2 (Alanine Transaminase); UR635J3F00 (saikosaponin)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170731
[Lr] Data última revisão:
170731
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170420
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-017-1733-0


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[PMID]:28412648
[Au] Autor:Wang S; Zhang Y; Zhang Q; Peng S; Shen C; Yu Y; Zhang M; Yang W; Wu Q; Zhang Y; Li S; Qiao Y
[Ad] Endereço:School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China.
[Ti] Título:Content decline of SERCA inhibitors saikosaponin a and d attenuates cardiotoxicity and hepatotoxicity of vinegar-baked Radix bupleuri.
[So] Source:Environ Toxicol Pharmacol;52:129-137, 2017 Jun.
[Is] ISSN:1872-7077
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Improper usage of unprocessed Radix bupleuri root (chaihu) may cause cardiotoxicity and liver injury. Baking herb with vinegar is believed to attenuate the adverse responses. However, the chemical and molecular basis involved remained unclear. To this end, we investigated the in vitro toxicity of saikosaponin a, c, d, and their hydrolysates saikosaponin b1 and b2. Results showed that SSa and SSd possessed higher affinity with sarcoplasmic/endoplasmic reticulum calcium ATPase (SERCA) by molecular docking, and exhibited stronger toxic responses on cardiomyocytes and hepatocytes than the other three saikosaponins in equivalent concentrations. Further, SSa and SSd induced LC3 puncta formation in U2OS-mCherry-EGFP-LC3 cells. Blockage of autophagy by 3-methyladenine did not abrogate the cytotoxicities induced by SSa and SSd. In parallel, none of SSc, SSb1, or SSb2 caused cell injury. Our study reveals how changes in chemical ingredients are connected to the toxicity of Chaihu during vinegar baking process and also provides a guidance for structure optimization to reduce drug induced toxicity.
[Mh] Termos MeSH primário: Cardiotoxicidade/prevenção & controle
Doença Hepática Induzida por Substâncias e Drogas/prevenção & controle
Miócitos Cardíacos/efeitos dos fármacos
Ácido Oleanólico/análogos & derivados
Preparações de Plantas/toxicidade
Saponinas/toxicidade
ATPases Transportadoras de Cálcio do Retículo Sarcoplasmático/antagonistas & inibidores
[Mh] Termos MeSH secundário: Ácido Acético/química
Animais
Animais Recém-Nascidos
Apoptose/efeitos dos fármacos
Bupleurum
Linhagem Celular
Células Cultivadas
Feminino
Células Hep G2
Temperatura Alta
Seres Humanos
Masculino
Miócitos Cardíacos/fisiologia
Ácido Oleanólico/química
Ácido Oleanólico/toxicidade
Preparações de Plantas/química
Raízes de Plantas
Ratos Sprague-Dawley
Saponinas/química
ATPases Transportadoras de Cálcio do Retículo Sarcoplasmático/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Preparations); 0 (Saponins); 6SMK8R7TGJ (Oleanolic Acid); EC 3.6.3.8 (Sarcoplasmic Reticulum Calcium-Transporting ATPases); Q40Q9N063P (Acetic Acid); UR635J3F00 (saikosaponin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170417
[St] Status:MEDLINE


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[PMID]:28343034
[Au] Autor:Zengin G; Bulut G; Mollica A; Haznedaroglu MZ; Dogan A; Aktumsek A
[Ad] Endereço:Selcuk University, Science Faculty, Department of Biology, Campus, 42250, Konya, Turkey. Electronic address: gokhanzengin@selcuk.edu.tr.
[Ti] Título:Bioactivities of Achillea phrygia and Bupleurum croceum based on the composition of phenolic compounds: In vitro and in silico approaches.
[So] Source:Food Chem Toxicol;107(Pt B):597-608, 2017 Sep.
[Is] ISSN:1873-6351
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This study presents the effects of the Achillea phrygia Boiss. et Bal. and Bupleurum croceum Fenzl. extracts obtained by different solvents (ethyl acetate, methanol and water) on selected enzyme inhibitory effects and antioxidant ability with screening bioactive compounds. Total and individual bioactive compounds were detected by spectrophotometric and HPLC-DAD techniques. Antioxidant abilities were evaluated by different methods including free radical scavenging (ABTS and DPPH), reducing power (CUPRAC and FRAP), phosphomolybdenum and metal chelating. Enzyme inhibitory effects were tested against cholinesterases, tyrosinase, amylase, glucosidase and lipase. Total phenolic contents were ranged from 20.52 mgGAE/g extract (B. croceum methanol extract) to 41.13 mgGAE/g extract (A. phrygia methanol extract). Generally, methanol and water extracts showed the strongest antioxidant abilities, while the ethyl acetate extracts had the most promising enzyme inhibitory effects. HPLC analysis revealed the abundance of some phenolics including rutin, quercetin, sinapic acid and chlorogenic acid, respectively. These components were also assessed using molecular modelling with the aim to study their docking properties on a set of six enzymes used in this study. Overall, these species could be suggested as valuable sources of natural-bioactive agents for developing new functional, pharmacological and health-promoting ingredients.
[Mh] Termos MeSH primário: Achillea/química
Bupleurum/química
Fenóis/química
Extratos Vegetais/química
[Mh] Termos MeSH secundário: Antioxidantes/química
Cromatografia Líquida de Alta Pressão
Simulação por Computador
Modelos Moleculares
Estrutura Molecular
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Phenols); 0 (Plant Extracts)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170907
[Lr] Data última revisão:
170907
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170327
[St] Status:MEDLINE


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[PMID]:28314599
[Au] Autor:Fang W; Yang YJ; Guo BL; Cen S
[Ad] Endereço:Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, People's Republic of China.
[Ti] Título:Anti-influenza triterpenoid saponins (saikosaponins) from the roots of Bupleurum marginatum var. stenophyllum.
[So] Source:Bioorg Med Chem Lett;27(8):1654-1659, 2017 04 15.
[Is] ISSN:1464-3405
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This study to investigate antiviral components from the roots of Bupleurum marginatum var. stenophyllum led to the isolation of five novel saikosaponins, namely 6″-O-crotonyl-saikosaponin a (1), tibesaikosaponin I (2), tibesaikosaponin II (3), tibesaikosaponin III (4), tibesaikosaponin IV (5), along with 9 known analogues (6-14). Their structures were established by spectral data analyses (IR, MS, 1D and 2D NMR) and by comparison of spectral data with those of the related known compounds. Antiviral testing of all compounds against influenza A virus A/WSN/33 (H1N1) in 293TGluc cells showed that nepasaikosaponin k (12), saikosaponin n (13) and saikosaponin h (14) behaved more potent inhibitory activity and selectivity than the positive control, Ribavirin. The preliminary structure-activity relationship studies suggest that the 13, 28-epoxy group, the type of sugar chain and the type of olefinic bonds are significant for antiviral activity and selectivity.
[Mh] Termos MeSH primário: Antivirais/química
Antivirais/farmacologia
Bupleurum/química
Vírus da Influenza A Subtipo H1N1/efeitos dos fármacos
Ácido Oleanólico/análogos & derivados
Saponinas/química
Saponinas/farmacologia
[Mh] Termos MeSH secundário: Antivirais/isolamento & purificação
Linhagem Celular
Seres Humanos
Influenza Humana/tratamento farmacológico
Ácido Oleanólico/química
Ácido Oleanólico/isolamento & purificação
Ácido Oleanólico/farmacologia
Raízes de Plantas/química
Saponinas/isolamento & purificação
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiviral Agents); 0 (Saponins); 6SMK8R7TGJ (Oleanolic Acid); UR635J3F00 (saikosaponin)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171124
[Lr] Data última revisão:
171124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170319
[St] Status:MEDLINE


  9 / 296 MEDLINE  
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[PMID]:28288911
[Au] Autor:Wu YS; Lee MC; Huang CT; Kung TC; Huang CY; Nan FH
[Ad] Endereço:Department of Aquaculture, National Taiwan Ocean University, Keelung 20248, Taiwan, ROC.
[Ti] Título:Effects of traditional medical herbs "minor bupleurum decoction" on the non-specific immune responses of white shrimp (Litopenaeus vannamei).
[So] Source:Fish Shellfish Immunol;64:218-225, 2017 May.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This study is investigating the effect of minor bupleurum decoction (Xiao-Chai-Hu decoction) on the non-specific immune response of white shrimp (Litopenaeus vannamei). To determine prophenoloxidase activity (proPO), reactive oxygen species production (ROS), superoxide anion production (O ), nitric oxide production (NO), phagocytic rate (PR), phagocytic index (PI), superoxide dismutase activity (SOD), total haemocyte count (THC) and differential haemocyte count (DHC). In this experiment, treating with different dosages (0, 0.25, 0.5 and, 1%) of minor bupleurum decoction to detect immune parameters on day 0, 1, 2, 4, 7, 14, 21 and 28. Result is shown that 0.25% treatment significantly enhanced the superoxide dismutase (SOD) activity and, 0.25 and 1% treatment significantly increased the ROS production, nitric oxide (NO) production and phagocytic rate (PR) moreover, 0.5 and 1% treatment induced the proPO activity and superoxide anion (O ) production. Evidence exactly indicated that minor bupleurum decoction is able to enhance the non-specific immunity responses of white shrimp via in vivo examination.
[Mh] Termos MeSH primário: Bupleurum/química
Medicamentos de Ervas Chinesas/farmacologia
Imunidade Inata/efeitos dos fármacos
Penaeidae/efeitos dos fármacos
Penaeidae/imunologia
[Mh] Termos MeSH secundário: Animais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drugs, Chinese Herbal); 0 (xiaochaihu)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170628
[Lr] Data última revisão:
170628
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170315
[St] Status:MEDLINE


  10 / 296 MEDLINE  
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[PMID]:28231747
[Au] Autor:Chen CH; Chen MF; Huang SJ; Huang CY; Wang HK; Hsieh WC; Huang CH; Liu LF; Shiu LY
[Ad] Endereço:* Institute for Translational Research in Biomedicine, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan.
[Ti] Título:Saikosaponin a Induces Apoptosis through Mitochondria-Dependent Pathway in Hepatic Stellate Cells.
[So] Source:Am J Chin Med;45(2):351-368, 2017.
[Is] ISSN:0192-415X
[Cp] País de publicação:Singapore
[La] Idioma:eng
[Ab] Resumo:Saikosaponin a (SSa) is one of the main active components of Bupleurum falcatum. It is commonly used to treat liver injury and fibrosis in traditional Chinese medicine. Our previous study showed that SSa induces apoptosis and inhibits the proliferation of rat hepatic stellate cell (HSC) line HSC-T6. The aim of the present study was to elucidate the mechanism of SSa-mediated apoptosis. Rat HSC cell line HSC-T6 and human HSC cell line LX-2 were used in this study. SSa triggered cell death mainly by apoptosis, as indicated by the typical morphological changes, sub-G1 phase of cell cycle increase, and activation of the caspase-9/caspase-3 cascade. In addition, SSa-induced apoptosis was partially inhibited by the caspase-3 inhibitor Z-DEVD-FMK, suggesting an involvement of caspase-3 dependent and independent pathways. Moreover, SSa upregulated pro-apoptotic proteins [BAK, Bcl-2-associated death promoter (BAD), and p53 upregulated modulator of apoptosis (PUMA)] and downregulated anti-apoptotic proteins (Bcl-2). In the mitochondria, SSa triggered the translocation of BAX and BAK from the cytosol to the outer membrane, resulting in a reduction of mitochondrial functions and membrane potential and subsequent release of apoptotic factors. Therefore, this study demonstrates that SSa induces apoptosis through the intrinsic mitochondrial-dependent pathway in HSCs.
[Mh] Termos MeSH primário: Apoptose/efeitos dos fármacos
Células Estreladas do Fígado/citologia
Mitocôndrias/metabolismo
Ácido Oleanólico/análogos & derivados
Saponinas/farmacologia
[Mh] Termos MeSH secundário: Animais
Apoptose/genética
Bupleurum
Caspase 3/metabolismo
Proliferação Celular/efeitos dos fármacos
Proliferação Celular/genética
Células Cultivadas
Ácido Oleanólico/farmacologia
Ratos
Estimulação Química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Saponins); 6SMK8R7TGJ (Oleanolic Acid); EC 3.4.22.- (Caspase 3); UR635J3F00 (saikosaponin)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170508
[Lr] Data última revisão:
170508
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170225
[St] Status:MEDLINE
[do] DOI:10.1142/S0192415X17500227



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