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[PMID]:28407236
[Au] Autor:Duncan SE; Moberg K; Amin KN; Wright M; Newkirk JJ; Ponder MA; Acuff GR; Dickson JS
[Ad] Endereço:Food Science and Technology Dept., Human and Agricultural Biosciences Building, 1230 Washington St. SW., Virginia Tech, Blacksburg, Va, 24061, U.S.A.
[Ti] Título:Processes to Preserve Spice and Herb Quality and Sensory Integrity During Pathogen Inactivation.
[So] Source:J Food Sci;82(5):1208-1215, 2017 May.
[Is] ISSN:1750-3841
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Selected processing methods, demonstrated to be effective at reducing Salmonella, were assessed to determine if spice and herb quality was affected. Black peppercorn, cumin seed, oregano, and onion powder were irradiated to a target dose of 8 kGy. Two additional processes were examined for whole black peppercorns and cumin seeds: ethylene oxide (EtO) fumigation and vacuum assisted-steam (82.22 °C, 7.5 psia). Treated and untreated spices/herbs were compared (visual, odor) using sensory similarity testing protocols (α = 0.20; ß = 0.05; proportion of discriminators: 20%) to determine if processing altered sensory quality. Analytical assessment of quality (color, water activity, and volatile chemistry) was completed. Irradiation did not alter visual or odor sensory quality of black peppercorn, cumin seed, or oregano but created differences in onion powder, which was lighter (higher L ) and more red (higher a ) in color, and resulted in nearly complete loss of measured volatile compounds. EtO processing did not create detectable odor or appearance differences in black peppercorn; however visual and odor sensory quality differences, supported by changes in color (higher b ; lower L ) and increased concentrations of most volatiles, were detected for cumin seeds. Steam processing of black peppercorn resulted in perceptible odor differences, supported by increased concentration of monoterpene volatiles and loss of all sesquiterpenes; only visual differences were noted for cumin seed. An important step in process validation is the verification that no effect is detectable from a sensory perspective.
[Mh] Termos MeSH primário: Cuminum/química
Óxido de Etileno/farmacologia
Piper nigrum/química
Especiarias/análise
Vapor
[Mh] Termos MeSH secundário: Monoterpenos/análise
Sementes/química
Sesquiterpenos/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Monoterpenes); 0 (Sesquiterpenes); 0 (Steam); JJH7GNN18P (Ethylene Oxide)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170414
[St] Status:MEDLINE
[do] DOI:10.1111/1750-3841.13702


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[PMID]:28062287
[Au] Autor:Wang Y; Sun Y; Han L; Zhang X; Feng J
[Ad] Endereço:Research and Development Center of Biorational Pesticides, Northwest A & F University, Yangling 712100, Shaanxi, China.
[Ti] Título:Potential use of cuminic acid as a botanical fungicide against Valsa mali.
[So] Source:Microb Pathog;106:9-15, 2017 May.
[Is] ISSN:1096-1208
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Valsa canker caused by Valsa mali is commonly present in eastern Asia and cause large economic losses. Because of limited agricultural measures and chemical residues of commonly used fungicides there is an urgent need of alternative plant protecting agents. On this background the activity of cuminic acid, a plant extract from the seed of Cuminum cyminum L, was assessed. The median effective concentration (EC ) values for inhibition of mycelial growth of seven V. mali strains ranged from 3.046 to 8.342 µg/mL, with an average EC value of 4.956 ± 0.281 µg/mL. The antifungal activity was the direct activity of cuminic acid instead of the influence on the pH of media by cuminic acid. After treated with cuminic acid, mycelia dissolved with decreased branches and swelling; cell membrane permeability increased while pectinases activity decreased significantly. Moreover, peroxidase (POD) activity of the apple leaves increased after treated with cuminic acid. Importantly, on detached branches of apple tree, cuminic acid exhibited both protective and curative activity. These results indicated that cuminic acid not only showed the antifungal activity, but also could improve the defense capacity of the plants. Taken together, cuminic acid showed the potential as a natural alternative to commercial fungicides or a lead compound to develop new fungicides for the control of Valsa canker.
[Mh] Termos MeSH primário: Antifúngicos/farmacologia
Doenças das Plantas/prevenção & controle
Saccharomycetales/efeitos dos fármacos
Saccharomycetales/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Antifúngicos/administração & dosagem
Antifúngicos/química
Permeabilidade da Membrana Celular/efeitos dos fármacos
Meios de Cultura/química
Cuminum/química
Fungicidas Industriais/química
Fungicidas Industriais/farmacologia
Concentração de Íons de Hidrogênio
Malus/efeitos dos fármacos
Malus/microbiologia
Microscopia Eletrônica de Varredura
Micélio/citologia
Micélio/efeitos dos fármacos
Micélio/crescimento & desenvolvimento
Ácido Oxálico/análise
Peroxidase/efeitos dos fármacos
Doenças das Plantas/microbiologia
Extratos Vegetais/farmacologia
Folhas de Planta/efeitos dos fármacos
Folhas de Planta/enzimologia
Poligalacturonase/efeitos dos fármacos
Saccharomycetales/patogenicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Culture Media); 0 (Fungicides, Industrial); 0 (Plant Extracts); 9E7R5L6H31 (Oxalic Acid); EC 1.11.1.7 (Peroxidase); EC 3.2.1.15 (Polygalacturonase)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171108
[Lr] Data última revisão:
171108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170108
[St] Status:MEDLINE


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[PMID]:28044460
[Au] Autor:Farvardin A; Ebrahimi A; Hosseinpour B; Khosrowshahli M
[Ad] Endereço:a Department of Plant Biotechnology, Science and Research Branch , Islamic Azad university , Tehran , Iran.
[Ti] Título:Effects of growth regulators on callus induction and secondary metabolite production in Cuminum cyminum.
[So] Source:Nat Prod Res;31(17):1963-1970, 2017 Sep.
[Is] ISSN:1478-6427
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Cumin (Cuminum cyminum) is an annual plant from Apiaceae family that is cultivated in Iran as landraces. The most important chemical composition of the cumin essential oil was cuminaldehyde. In this research, the effect of different landraces and growth regulators was evaluated on callus induction, and best callus was used for amount of cuminaldehyde content. Node, root, leaf and hypocotyl explant from seedlings of Birjand and Qaen landraces were cultured on MS and MS5 medium supplemented with different concentrations of 2, 4-D and Kin. This experiment has been carried out in a completely randomised design with 3 replications. Percentage of callogenesis, callus volume, fresh and dry weight were measured. The best treatment for callus induction was 2.5 mg/L 2, 4-D and 0.5 mg/L Kin in MS5 medium. The best callus result was evaluated for cuminaldehyde content. An amount of 5.7% cuminaldehyde was measured using hydrodistillation method.
[Mh] Termos MeSH primário: Benzaldeídos/isolamento & purificação
Cuminum/efeitos dos fármacos
Cuminum/metabolismo
Reguladores de Crescimento de Planta/farmacologia
Metabolismo Secundário/efeitos dos fármacos
[Mh] Termos MeSH secundário: Benzaldeídos/metabolismo
Cromatografia Gasosa
Meios de Cultura
Cuminum/citologia
Hipocótilo/citologia
Irã (Geográfico)
Óleos Voláteis/química
Folhas de Planta/citologia
Folhas de Planta/efeitos dos fármacos
Folhas de Planta/metabolismo
Raízes de Plantas/citologia
Raízes de Plantas/efeitos dos fármacos
Raízes de Plantas/metabolismo
Plântulas/citologia
Plântulas/efeitos dos fármacos
Plântulas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzaldehydes); 0 (Culture Media); 0 (Oils, Volatile); 0 (Plant Growth Regulators); O0893NC35F (cuminaldehyde)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170104
[St] Status:MEDLINE
[do] DOI:10.1080/14786419.2016.1272105


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[PMID]:27937075
[Au] Autor:Gómez-Mateos Pérez M; Navarro Moll C; Merino Espinosa G; Valero López A
[Ad] Endereço:a Department of Parasitology, Faculty of Pharmacy , University of Granada , Granada , Spain.
[Ti] Título:Evaluation of different Mediterranean essential oils as prophylactic agents in anisakidosis.
[So] Source:Pharm Biol;55(1):456-461, 2017 Dec.
[Is] ISSN:1744-5116
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:CONTEXT: Anisakis Dujardin 1845 (Anisakidae) nematodes can cause gastrointestinal and allergic diseases when humans eat raw or undercooked seafood containing larvae. There is currently no drug available in the market against this parasitic disease, and the study of plant-derived molecules could be useful in the discovery of effective compounds. OBJECTIVE: This research assesses the biocidal activity of a range of essential oils (EOs) from some Mediterranean plants against larvae found in the musculature of fresh fish. MATERIALS AND METHODS: EOs composition was analyzed by gas chromatography-mass spectroscopy. All the EOs were diluted at 5% v/v in olive oil to cover the fish with the solutions for 24 h. The larvae that abandoned the muscle and the larvae obtained from the artificial digestion of the fish were collected. Controls were carried out in parallel. Furthermore, Wistar rats were infected with the live larvae collected from the in vitro trials in order to find any larvae that may have penetrated the gastrointestinal wall. RESULTS: Between 60.8% and 87.6% of parasites treated with EOs abandoned the fish muscle, and the highest in vitro mortality rate was achieved with oregano EO (53.9%). Rats previously treated with oregano, cumin and Spanish lavender EOs showed no detectable lesions in the digestive tract due to the infection with larvae. CONCLUSIONS: Oregano (Origanum vulgare L. Lamiaceae), cumin (Cuminum cyminum L. Apiaceae) and Spanish lavender (Lavender stoechas L. Lamiaceae) EOs could be used as promising ingredients in the development of products for the control of anisakiasis.
[Mh] Termos MeSH primário: Anisaquíase/prevenção & controle
Anisakis/efeitos dos fármacos
Antinematódeos/farmacologia
Cuminum/química
Óleos Voláteis/farmacologia
Origanum/química
Extratos Vegetais/farmacologia
Óleos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Animais
Anisaquíase/parasitologia
Anisakis/crescimento & desenvolvimento
Antinematódeos/isolamento & purificação
Peixes
Cromatografia Gasosa-Espectrometria de Massas
Larva/efeitos dos fármacos
Região do Mediterrâneo
Óleos Voláteis/isolamento & purificação
Fitoterapia
Extratos Vegetais/isolamento & purificação
Óleos Vegetais/isolamento & purificação
Plantas Medicinais
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antinematodal Agents); 0 (Oils, Volatile); 0 (Plant Extracts); 0 (Plant Oils); ZBP1YXW0H8 (lavender oil)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170309
[Lr] Data última revisão:
170309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161213
[St] Status:MEDLINE


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[PMID]:27830269
[Au] Autor:Kakarla P; Floyd J; Mukherjee M; Devireddy AR; Inupakutika MA; Ranweera I; Kc R; 'Shrestha U; Cheeti UR; Willmon TM; Adams J; Bruns M; Gunda SK; Varela MF
[Ad] Endereço:Department of Biology, Eastern New Mexico University, Portales, NM, 88130, USA.
[Ti] Título:Inhibition of the multidrug efflux pump LmrS from Staphylococcus aureus by cumin spice Cuminum cyminum.
[So] Source:Arch Microbiol;199(3):465-474, 2017 Apr.
[Is] ISSN:1432-072X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Staphylococcus aureus is a serious causative agent of infectious disease. Multidrug-resistant strains like methicillin-resistant S. aureus compromise treatment efficacy, causing significant morbidity and mortality. Active efflux represents a major antimicrobial resistance mechanism. The proton-driven multidrug efflux pump, LmrS, actively exports structurally distinct antimicrobials. To circumvent resistance and restore clinical efficacy of antibiotics, efflux pump inhibitors are necessary, and natural edible spices like cumin are potential candidates. The mode of cumin antibacterial action and underlying mechanisms behind drug resistance inhibition, however, are unclear. We tested the hypothesis that cumin inhibits LmrS drug transport. We found that cumin inhibited bacterial growth and LmrS ethidium transport in a dosage-dependent manner. We demonstrate that cumin is antibacterial toward a multidrug-resistant host and that resistance modulation involves multidrug efflux inhibition.
[Mh] Termos MeSH primário: Cuminum/química
Farmacorresistência Bacteriana Múltipla/efeitos dos fármacos
Genes MDR/fisiologia
Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos
Extratos Vegetais/farmacologia
Staphylococcus aureus/efeitos dos fármacos
[Mh] Termos MeSH secundário: Antibacterianos/farmacologia
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Farmacorresistência Bacteriana Múltipla/genética
Etídio/metabolismo
Genes MDR/genética
Testes de Sensibilidade Microbiana
Proteínas Associadas à Resistência a Múltiplos Medicamentos/antagonistas & inibidores
Infecções Estafilocócicas/microbiologia
Staphylococcus aureus/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 0 (Multidrug Resistance-Associated Proteins); 0 (Plant Extracts); EN464416SI (Ethidium)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170530
[Lr] Data última revisão:
170530
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161111
[St] Status:MEDLINE
[do] DOI:10.1007/s00203-016-1314-5


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[PMID]:27751723
[Au] Autor:Minooeianhaghighi MH; Sepehrian L; Shokri H
[Ad] Endereço:Department of Basic Sciences, Faculty of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran.
[Ti] Título:Antifungal effects of Lavandula binaludensis and Cuminum cyminum essential oils against Candida albicans strains isolated from patients with recurrent vulvovaginal candidiasis.
[So] Source:J Mycol Med;27(1):65-71, 2017 Mar.
[Is] ISSN:1773-0449
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: Recurrent vulvovaginal candidiasis (RVVC), which affects approximately 5% of women of reproductive age, is defined as 4 or more episodes of symptomatic Candida vaginitis within a year. The purposes of this study were to determine the chemical compositions and antifungal susceptibility of Cuminum cyminum (C. cyminum) and Lavandula binaludensis (L. binaludensis) essential oils and their combination against Candida albicans (C. albicans) strains isolated from patients with RVVC. MATERIALS AND METHODS: C. albicans isolates were identified via germ tube test, CHROMagar and RapID Yeast Plus System. The essential oils were obtained by hydrodistillation in a Clevenger apparatus and analyzed by gas chromatography-mass spectroscopy (GC-MS). The broth microdilution method was used as antifungal susceptibility test (CLSI-M27-A3). RESULTS: The GC-MS analysis allowed 13 components to be determined; the main components of C. cyminum and L. binaludensis essential oils were γ-terpinene (21.07%) and 1,8-cineole (71.56%), respectively. L. binaludensis and C. cyminum oils were effective in inhibiting C. albicans growth at mean concentrations of 7.91±1.61µg/mL and 8.00±1.89µg/mL, respectively. In addition, the combination of C. cyminum with L. binaludensis oils were more active causing inhibition in all C. albicans isolates, with concentrations varying from 3.90 to 11.71µg/mL (mean value: 7.22±1.69µg/mL). CONCLUSION: The results suggested the potential substitution of the antifungal chemicals by C. cyminum and L. binaludensis essential oils as natural inhibitors to control the growth of the most important pathogenic Candida species and alternative therapies for RVVC.
[Mh] Termos MeSH primário: Antifúngicos/farmacologia
Candida albicans/efeitos dos fármacos
Candidíase Vulvovaginal/microbiologia
Cuminum/química
Lavandula/química
Óleos Voláteis/farmacologia
Óleos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Antifúngicos/isolamento & purificação
Candida albicans/isolamento & purificação
Feminino
Cromatografia Gasosa-Espectrometria de Massas
Seres Humanos
Testes de Sensibilidade Microbiana
Óleos Voláteis/isolamento & purificação
Óleos Vegetais/isolamento & purificação
Recidiva
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Oils, Volatile); 0 (Plant Oils)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161019
[St] Status:MEDLINE


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[PMID]:27507449
[Au] Autor:Siow HL; Lim TS; Gan CY
[Ad] Endereço:Analytical Biochemistry Research Centre, Universiti Sains Malaysia, 11800 USM, Penang, Malaysia.
[Ti] Título:Development of a workflow for screening and identification of α-amylase inhibitory peptides from food source using an integrated Bioinformatics-phage display approach: Case study - Cumin seed.
[So] Source:Food Chem;214:67-76, 2017 Jan 01.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The main objective of this study was to develop an efficient workflow to discover α-amylase inhibitory peptides from cumin seed. A total of 56 unknown peptides was initially found in the cumin seed protein hydrolysate. They were subjected to 2 different in silico screenings and 6 peptides were shortlisted. The peptides were then subjected to in vitro selection using phage display technique and 3 clones (CSP3, CSP4 and CSP6) showed high affinity in binding α-amylase. These clones were subjected to the inhibitory test and only CSP4 and CSP6 exhibited high inhibitory activity. Therefore, these peptides were chemically synthesized for validation purposes. CSP4 exhibited inhibition of bacterial and human salivary α-amylases with IC50 values of 0.11 and 0.04µmol, respectively, whereas CSP6 was about 0.10 and 0.15µmol, respectively. Results showed that the strength of each protocol has been successfully combined as deemed fit to enhance the α-amylase inhibitor peptide discovery.
[Mh] Termos MeSH primário: Bacteriófagos
Biologia Computacional/métodos
Cuminum
Fragmentos de Peptídeos/análise
Fluxo de Trabalho
alfa-Amilases/antagonistas & inibidores
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Bacteriófagos/genética
Bacteriófagos/metabolismo
Cuminum/genética
Cuminum/metabolismo
Seres Humanos
Fragmentos de Peptídeos/genética
Fragmentos de Peptídeos/metabolismo
Sementes/genética
Sementes/metabolismo
alfa-Amilases/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Peptide Fragments); EC 3.2.1.1 (alpha-Amylases)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:171008
[Lr] Data última revisão:
171008
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160811
[St] Status:MEDLINE


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[PMID]:26560460
[Au] Autor:Jessica Elizabeth T; Gassara F; Kouassi AP; Brar SK; Belkacemi K
[Ad] Endereço:a INRS-ETE, Université du Québec , Québec , Canada.
[Ti] Título:Spice use in food: Properties and benefits.
[So] Source:Crit Rev Food Sci Nutr;57(6):1078-1088, 2017 Apr 13.
[Is] ISSN:1549-7852
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Spices are parts of plants that due to their properties are used as colorants, preservatives, or medicine. The uses of spices have been known since long time, and the interest in the potential of spices is remarkable due to the chemical compounds contained in spices, such as phenylpropanoids, terpenes, flavonoids, and anthocyanins. Spices, such as cumin (cuminaldehyde), clove (eugenol), and cinnamon (cinnamaldehyde) among others, are known and studied for their antimicrobial and antioxidant properties due to their main chemical compounds. These spices have the potential to be used as preservatives in many foods namely in processed meat to replace chemical preservatives. Main chemical compounds in spices also confer other properties providing a variety of applications to spices, such as insecticidal, medicines, colorants, and natural flavoring. Spices provide beneficial effects, such as antioxidant activity levels that are comparable to regular chemical antioxidants used so they can be used as a natural alternative to synthetic preservatives. In this review, the main characteristics of spices will be described as well as their chemical properties, different applications of these spices, and the advantages and disadvantages of their use.
[Mh] Termos MeSH primário: Especiarias/análise
[Mh] Termos MeSH secundário: Antocianinas/análise
Antocianinas/química
Anti-Infecciosos/análise
Anti-Infecciosos/química
Antioxidantes/análise
Antioxidantes/química
Cinnamomum zeylanicum/química
Cuminum/química
Flavonoides/análise
Flavonoides/química
Corantes de Alimentos/análise
Conservantes de Alimentos/análise
Conservantes de Alimentos/química
Gengibre/química
Inseticidas/análise
Inseticidas/química
Piper nigrum/química
Extratos Vegetais/análise
Extratos Vegetais/química
Rosmarinus/química
Syzygium/química
Terpenos/análise
Terpenos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Anthocyanins); 0 (Anti-Infective Agents); 0 (Antioxidants); 0 (Flavonoids); 0 (Food Coloring Agents); 0 (Food Preservatives); 0 (Insecticides); 0 (Plant Extracts); 0 (Terpenes)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170315
[Lr] Data última revisão:
170315
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151113
[St] Status:MEDLINE
[do] DOI:10.1080/10408398.2013.858235


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[PMID]:27808391
[Au] Autor:Abdelhaliem E; Al-Huqail AA
[Ad] Endereço:Plant Molecular Genetic and Cytogenetic, Botany Department, Science College, Zagazig University, Sharkia, Egypt ekram@ksu.edu.sa.
[Ti] Título:Genetic linkage between protein and DNA polymorphisms and antioxidant capacity of Cuminum cyminum L. accessions.
[So] Source:Genet Mol Res;15(4), 2016 Oct 06.
[Is] ISSN:1676-5680
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:This study aimed to link the genetic variation observed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and random amplified polymorphic DNA (RAPD) analysis among 11 Cuminum cyminum L. accessions, collected from diverse ecogeographical areas in Saudi Arabia, with their antioxidant capacity to better identify potential genotypes for breeding programs for this medicinal spice. SDS-PAGE analysis revealed genetic variation among cumin germplasms and distinct polymorphisms (100%). Protein polymorphisms were identified based on the number of polypeptide bands (288) with molecular weights ranging from 13.85 to 350 kDa, band intensity, the appearance of new bands, and the absence of other bands. RAPD analysis revealed 363 amplified DNA products with a high polymorphism value (98.88%) based on DNA band type (unique, non-unique, and monomorphic), DNA 90 to 1085-bp long, and band intensity. The unweighted pair group method with arithmetic mean clustering based on SDS-PAGE or RAPD and Jaccard's similarity coefficient divided cumin accessions into similar but distinct clusters with respect to their location of collection. The antioxidant potential of cumin accessions based on 1, 1-diphenyl-2-picrylhydrazyl radical scavenging activity, the ß-carotene-linoleate model system, and total phenolic and flavonoid contents revealed distinct variability. These data indicate that cumin is a valuable genetic resource with high antioxidant activity. Additionally, clustering based on antioxidant activity was not identical to that based on SDS-PAGE and RAPD. Data and clustering of SDS-PAGE and RAPD, combined with the high antioxidant capacity of cumin accessions, are important for the efficient use of genetic resources of cumin in breeding strategies and genetic improvement programs.
[Mh] Termos MeSH primário: Cuminum/genética
Cuminum/metabolismo
[Mh] Termos MeSH secundário: Antioxidantes/metabolismo
Impressões Digitais de DNA
Eletroforese em Gel de Poliacrilamida/métodos
Ligação Genética
Variação Genética
Genótipo
Oxirredução
Melhoramento Vegetal
Polimorfismo Genético
Técnica de Amplificação ao Acaso de DNA Polimórfico/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170321
[Lr] Data última revisão:
170321
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161104
[St] Status:MEDLINE
[do] DOI:10.4238/gmr.15048916


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[PMID]:27802299
[Au] Autor:Sheweita SA; El-Hosseiny LS; Nashashibi MA
[Ad] Endereço:Department of Biotechnology, Institute of Graduate Studies and Research, Alexandria University, Alexandria, Egypt.
[Ti] Título:Protective Effects of Essential Oils as Natural Antioxidants against Hepatotoxicity Induced by Cyclophosphamide in Mice.
[So] Source:PLoS One;11(11):e0165667, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Clinical application of cyclophosphamide (CP) as an anticancer drug is often limited due to its toxicity. CP is metabolized mainly in the liver by cytochrome P450 system into acrolein which is the proximate toxic metabolite. Many different natural antioxidants were found to alleviate the toxic effects of various toxic agents via different mechanisms. Therefore, the present study aimed at investigating the role of essential oils extracted from fennel, cumin and clove as natural antioxidants in the alleviation of hepatotoxicity induced by CP through assessment of hepatotoxicity biomarkers (AST, ALT, ALP), histopathology of liver tissues as well as other biochemical parameters involved in the metabolism of CP. The data of the present study showed that treatment of male mice with cyclophosphamide (2.5 mg/Kg BW) as repeated dose for 28 consecutive days was found to induce hepatotoxicity through the elevation in the activities of AST, ALT, and ALP. Combined administration of any of these oils with CP to mice partially normalized the altered hepatic biochemical markers caused by CP, whereas administration of fennel, clove or cumin essential oils alone couldn't change liver function indices. Moreover, CP caused histological changes in livers of mice including swelling and dilation in sinusoidal space, inflammation in portal tract and hepatocytes, as well as, hyperplasia in Kuppfer cells. However, co-administration of any of the essential oils with CP alleviated to some extent the changes caused by CP but not as the normal liver. CP was also found to induce free radical levels (measured as thiobarbituric acid reactive substances) and inhibited the activities of superoxide dismutase, glutathione reductase, and catalase as well as activities and protein expressions of both glutathione S-transferase (GSTπ) and glutathione peroxidase. Essential oils restored changes in activities of antioxidant enzymes (SOD, CAT, GR, GST, and GPx) caused by CP to their normal levels compared to control group. In addition, treatment of mice with CP was found to induce the protein expression of CYP 3A4, 2B1/2, 2C6, 2C23. Moreover, the present study showed that essential oils reduced the expression of CYPs 2E1, 3A4 but could not restore the expression of CYP 2C6 and 2C23 compared to CP-treated mice. Interestingly, pretreatment of mice with essential oil of clove was found to restore activities of DMN-dI, AHH, and ECOD which were induced by CP to their normal control levels. It is concluded that EOs showed a marked hepatoprotective effect against hepatotoxicity induced by CP. In addition, co-administration of CP with any of these oils might be used as a new strategy for cancer treatment to alleviate the hepatotoxicity induced by CP.
[Mh] Termos MeSH primário: Antioxidantes/uso terapêutico
Doença Hepática Induzida por Substâncias e Drogas/tratamento farmacológico
Doença Hepática Induzida por Substâncias e Drogas/patologia
Ciclofosfamida
Fígado/efeitos dos fármacos
Fígado/patologia
Óleos Voláteis/uso terapêutico
[Mh] Termos MeSH secundário: Animais
Antioxidantes/química
Antioxidantes/isolamento & purificação
Antioxidantes/farmacologia
Doença Hepática Induzida por Substâncias e Drogas/metabolismo
Cuminum/química
Sistema Enzimático do Citocromo P-450/metabolismo
Foeniculum/química
Fígado/metabolismo
Masculino
Camundongos
Óleos Voláteis/química
Óleos Voláteis/isolamento & purificação
Óleos Voláteis/farmacologia
Estresse Oxidativo/efeitos dos fármacos
Óleos Vegetais/química
Óleos Vegetais/isolamento & purificação
Óleos Vegetais/farmacologia
Óleos Vegetais/uso terapêutico
Syzygium/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Oils, Volatile); 0 (Plant Oils); 8N3DW7272P (Cyclophosphamide); 9035-51-2 (Cytochrome P-450 Enzyme System)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170628
[Lr] Data última revisão:
170628
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161102
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0165667



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