Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.075.750 [Categoria DeCS]
Referências encontradas : 223 [refinar]
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[PMID]:28873566
[Au] Autor:Dadan M; Rybak K; Wiktor A; Nowacka M; Zubernik J; Witrowa-Rajchert D
[Ad] Endereço:Department of Food Engineering and Process Management, Faculty of Food Sciences, Warsaw University of Life Sciences, Nowoursynowska 159c, 02-776 Warsaw, Poland. Electronic address: magdalena_dadan@sggw.pl.
[Ti] Título:Selected chemical composition changes in microwave-convective dried parsley leaves affected by ultrasound and steaming pre-treatments - An optimization approach.
[So] Source:Food Chem;239:242-251, 2018 Jan 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Parsley leaves contain a high amount of bioactive components (especially lutein), therefore it is crucial to select the most appropriate pre-treatment and drying conditions, in order to obtain high quality of dried leaves, which was the aim of this study. The optimization was done using response surface methodology (RSM) for the following factors: microwave power (100, 200, 300W), air temperature (20, 30, 40°C) and pre-treatment variant (ultrasound, steaming and dipping as a control). Total phenolic content (TPC), antioxidant activity, chlorophyll and lutein contents (using UPLC-PDA) were determined in dried leaves. The analysed responses were dependent on the applied drying parameters and the pre-treatment type. The possibility of ultrasound and steam treatment application was proven and the optimal processing conditions were selected.
[Mh] Termos MeSH primário: Petroselinum
[Mh] Termos MeSH secundário: Micro-Ondas
Fenóis
Folhas de Planta
Vapor
Ultrassom
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Phenols); 0 (Steam)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE


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[PMID]:28441391
[Au] Autor:Bauer D; Redmon N; Mazzio E; Soliman KF
[Ad] Endereço:College of Pharmacy and Pharmaceutical Sciences, Florida A & M University, Tallahassee, Florida, United States of America.
[Ti] Título:Apigenin inhibits TNFα/IL-1α-induced CCL2 release through IKBK-epsilon signaling in MDA-MB-231 human breast cancer cells.
[So] Source:PLoS One;12(4):e0175558, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mortality associated with breast cancer is attributable to aggressive metastasis, to which TNFα plays a central orchestrating role. TNFα acts on breast tumor TNF receptors evoking the release of chemotactic proteins (e.g. MCP-1/CCL2). These proteins direct inward infiltration/migration of tumor-associated macrophages (TAMs), tumor-associated neutrophils (TANs), myeloid-derived suppressor cells (MDSCs), T-regulatory cells (Tregs), T helper IL-17-producing cells (Th17s), metastasis-associated macrophages (MAMs) and cancer-associated fibroblasts (CAFs). Tumor embedded infiltrates collectively enable immune evasion, tumor growth, angiogenesis, and metastasis. In the current study, we investigate the potential of apigenin, a known anti-inflammatory constituent of parsley, to downregulate TNFα mediated release of chemokines from human triple-negative cells (MDA-MB-231 cells). The results show that TNFα stimulation leads to large rise of CCL2, granulocyte macrophage colony-stimulating factor (GMCSF), IL-1α and IL-6, all suppressed by apigenin. While many aspects of the transcriptome for NFkB signaling were evaluated, the data show signaling patterns associated with CCL2 were blocked by apigenin and mediated through suppressed mRNA and protein synthesis of IKBKe. Moreover, the data show that the attenuation of CCL2 by apigenin in the presence TNFα paralleled the suppression of phosphorylated extracellular signal-regulated kinase 1 (ERK 1/ 2). In summary, the obtained findings suggest that there exists a TNFα evoked release of CCL2 and other LSP recruiting cytokines from human breast cancer cells, which can be attenuated by apigenin.
[Mh] Termos MeSH primário: Anti-Inflamatórios/farmacologia
Apigenina/farmacologia
Neoplasias da Mama/tratamento farmacológico
Quimiocina CCL2/imunologia
Quinase I-kappa B/imunologia
Interleucina-1alfa/imunologia
Fator de Necrose Tumoral alfa/imunologia
[Mh] Termos MeSH secundário: Anti-Inflamatórios/química
Antineoplásicos Fitogênicos/química
Antineoplásicos Fitogênicos/farmacologia
Apigenina/química
Mama/efeitos dos fármacos
Mama/imunologia
Mama/patologia
Neoplasias da Mama/imunologia
Neoplasias da Mama/patologia
Linhagem Celular Tumoral
Sobrevivência Celular/efeitos dos fármacos
Feminino
Seres Humanos
Petroselinum/química
Transdução de Sinais/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Antineoplastic Agents, Phytogenic); 0 (CCL2 protein, human); 0 (Chemokine CCL2); 0 (IL1A protein, human); 0 (Interleukin-1alpha); 0 (Tumor Necrosis Factor-alpha); 7V515PI7F6 (Apigenin); EC 2.7.11.10 (I-kappa B Kinase); EC 2.7.11.10 (IKBKE protein, human)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170426
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0175558


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[PMID]:28319334
[Au] Autor:Fontana G; Gershlak J; Adamski M; Lee JS; Matsumoto S; Le HD; Binder B; Wirth J; Gaudette G; Murphy WL
[Ad] Endereço:Department of Orthopedics and Rehabilitation, University of Wisconsin School of Medicine and Public Health, Madison, WI, 53705, USA.
[Ti] Título:Biofunctionalized Plants as Diverse Biomaterials for Human Cell Culture.
[So] Source:Adv Healthc Mater;6(8), 2017 Apr.
[Is] ISSN:2192-2659
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The commercial success of tissue engineering products requires efficacy, cost effectiveness, and the possibility of scaleup. Advances in tissue engineering require increased sophistication in the design of biomaterials, often challenging the current manufacturing techniques. Interestingly, several of the properties that are desirable for biomaterial design are embodied in the structure and function of plants. This study demonstrates that decellularized plant tissues can be used as adaptable scaffolds for culture of human cells. With simple biofunctionalization technique, it is possible to enable adhesion of human cells on a diverse set of plant tissues. The elevated hydrophilicity and excellent water transport abilities of plant tissues allow cell expansion over prolonged periods of culture. Moreover, cells are able to conform to the microstructure of the plant frameworks, resulting in cell alignment and pattern registration. In conclusion, the current study shows that it is feasible to use plant tissues as an alternative feedstock of scaffolds for mammalian cells.
[Mh] Termos MeSH primário: Técnicas de Cultura de Células/métodos
Marantaceae/química
Células Mesenquimais Estromais/metabolismo
Petroselinum/química
Tecidos Suporte/química
[Mh] Termos MeSH secundário: Linhagem Celular
Seres Humanos
Células Mesenquimais Estromais/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170630
[Lr] Data última revisão:
170630
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170321
[St] Status:MEDLINE
[do] DOI:10.1002/adhm.201601225


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[PMID]:28317756
[Au] Autor:Samuoliene G; Virsile A; Brazaityte A; Jankauskiene J; Sakalauskiene S; Vastakaite V; Novickovas A; Viskeliene A; Sasnauskas A; Duchovskis P
[Ad] Endereço:Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, Kaunas Str. 30, Babtai, LT-54333, Kaunas Dist., Lithuania; Aleksandras Stulginskis University, Studentu 11, Akademija, LT-53361 Kaunas Distr., Lithuania. Electronic address: g.samuoliene@lsdi.lt.
[Ti] Título:Blue light dosage affects carotenoids and tocopherols in microgreens.
[So] Source:Food Chem;228:50-56, 2017 Aug 01.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Mustard, beet and parsley were grown to harvest time under selected LEDs: 638+660+731+0% 445nm; 638+660+731+8% 445nm; 638+660+731+16% 445nm; 638+660+731+25% 445nm; 638+660+731+33% 445nm. From 1.2 to 4.3 times higher concentrations of chlorophylls a and b, carotenoids, α- and ß-carotenes, lutein, violaxanthin and zeaxanthin was found under blue 33% treatment in comparison to lower blue light dosages. Meanwhile, the accumulation of metabolites, which were not directly connected with light reactions, such as tocopherols, was more influenced by lower (16%) blue light dosage, increasing about 1.3 times. Thus, microgreen enrichment of carotenoid and xanthophyll pigments may be achieved using higher (16-33%) blue light intensities. Changes in metabolite quantities were not the result of changes of other carotenoid concentration, but were more influenced by light treatment and depended on the species. Significant quantitative changes in response to blue light percentage were obtained for both directly and not directly light-dependent metabolite groups.
[Mh] Termos MeSH primário: Beta vulgaris/química
Carotenoides/química
Mostardeira/química
Petroselinum/química
Tocoferóis/química
[Mh] Termos MeSH secundário: Luz
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
36-88-4 (Carotenoids); R0ZB2556P8 (Tocopherols)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170321
[St] Status:MEDLINE


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[PMID]:28282402
[Au] Autor:Bell SM; Wendt DJ; Zhang Y; Taylor TW; Long S; Tsuruda L; Zhao B; Laipis P; Fitzpatrick PA
[Ad] Endereço:BioMarin Pharmaceutical, Novato, California, United States of America.
[Ti] Título:Formulation and PEGylation optimization of the therapeutic PEGylated phenylalanine ammonia lyase for the treatment of phenylketonuria.
[So] Source:PLoS One;12(3):e0173269, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Phenylketonuria (PKU) is a genetic metabolic disease in which the decrease or loss of phenylalanine hydroxylase (PAH) activity results in elevated, neurotoxic levels of phenylalanine (Phe). Due to many obstacles, PAH enzyme replacement therapy is not currently an option. Treatment of PKU with an alternative enzyme, phenylalanine ammonia lyase (PAL), was first proposed in the 1970s. However, issues regarding immunogenicity, enzyme production and mode of delivery needed to be overcome. Through the evaluation of PAL enzymes from multiple species, three potential PAL enzymes from yeast and cyanobacteria were chosen for evaluation of their therapeutic potential. The addition of polyethylene glycol (PEG, MW = 20,000), at a particular ratio to modify the protein surface, attenuated immunogenicity in an animal model of PKU. All three PEGylated PAL candidates showed efficacy in a mouse model of PKU (BTBR Pahenu2) upon subcutaneous injection. However, only PEGylated Anabaena variabilis (Av) PAL-treated mice demonstrated sustained low Phe levels with weekly injection and was the only PAL evaluated that maintained full enzymatic activity upon PEGylation. A PEGylated recombinant double mutant version of AvPAL (Cys503Ser/Cys565Ser), rAvPAL-PEG, was selected for drug development based on its positive pharmacodynamic profile and favorable expression titers. PEGylation was shown to be critical for rAvPAL-PEG efficacy as under PEGylated rAvPAL had a lower pharmacodynamic effect. rAvPAL and rAvPAL-PEG had poor stability at 4°C. L-Phe and trans-cinnamate were identified as activity stabilizing excipients. rAvPAL-PEG is currently in Phase 3 clinical trials to assess efficacy in PKU patients.
[Mh] Termos MeSH primário: Fenilalanina Amônia-Liase/uso terapêutico
Fenilcetonúrias/tratamento farmacológico
Polietilenoglicóis/química
[Mh] Termos MeSH secundário: Anabaena/enzimologia
Animais
Anticorpos/sangue
Modelos Animais de Doenças
Composição de Medicamentos
Terapia de Reposição de Enzimas
Ensaio de Imunoadsorção Enzimática
Camundongos
Nostoc/enzimologia
Petroselinum/enzimologia
Fenilalanina Amônia-Liase/química
Fenilalanina Amônia-Liase/imunologia
Fenilalanina Amônia-Liase/isolamento & purificação
Fenilcetonúrias/patologia
Proteínas Recombinantes/biossíntese
Proteínas Recombinantes/química
Proteínas Recombinantes/isolamento & purificação
Proteínas Recombinantes/uso terapêutico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies); 0 (Recombinant Proteins); 30IQX730WE (Polyethylene Glycols); EC 4.3.1.24 (Phenylalanine Ammonia-Lyase)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170829
[Lr] Data última revisão:
170829
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170311
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0173269


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[PMID]:28254010
[Au] Autor:El-Zaeddi H; Calín-Sánchez Á; Nowicka P; Martínez-Tomé J; Noguera-Artiaga L; Burló F; Wojdylo A; Carbonell-Barrachina ÁA
[Ad] Endereço:Universidad Miguel Hernández de Elche (UMH), Escuela Politécnica Superior de Orihuela (EPSO), Department of Agro-Food Technology, Research Group "Food Quality and Safety", Carretera Beniel, km 3.2, 03312-Orihuela, Alicante, Spain.
[Ti] Título:Preharvest treatments with malic, oxalic, and acetylsalicylic acids affect the phenolic composition and antioxidant capacity of coriander, dill and parsley.
[So] Source:Food Chem;226:179-186, 2017 Jul 01.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The effects of a preharvest treatment with malic (MA), oxalic (OA), or acetylsalicylic (ASA) acid at three concentrations (1, 2 and 3mM) on the bioactivity and antioxidant capacity of coriander, dill, and parsley were investigated. The antioxidant capacity of the herbs extracts was assayed by spectrophotometric methods by using three different analytical methods: ORAC, FRAP, and ABTS; the effects of treatments were very positive in coriander, produced intermediate results in dill, and no effects were found in parsley plants. Polyphenol compounds were identified by LC-MS-QTof and quantified by UPLC-PDA-FL. Thirty phenolic compounds were identified in these three herbs. The major compounds were (i) coriander: dimethoxycinnamoyl hexoside and quercetin-3-O-rutinoside, (ii) dill: neochlorogenic acid and quercetin glucuronide, and (iii) parsley: apigenin-7-apiosylglucoside (apiin) and isorhamnetin-3-O-hexoside. The application of these three organic acids favored the accumulation of phenolic compounds in coriander plants, but had no significant positive effects on dill and parsley. The treatments leading to the best results in all three plants were the application of MA or OA at 1mM.
[Mh] Termos MeSH primário: Anethum graveolens/química
Aspirina/química
Coriandrum/química
Malatos/química
Ácido Oxálico/química
Petroselinum/química
[Mh] Termos MeSH secundário: Antioxidantes
Fenóis
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Malates); 0 (Phenols); 817L1N4CKP (malic acid); 9E7R5L6H31 (Oxalic Acid); R16CO5Y76E (Aspirin)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170420
[Lr] Data última revisão:
170420
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170304
[St] Status:MEDLINE


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[PMID]:28040159
[Au] Autor:Jacobson AP; Wang H; Gill VS; Duvall R; Arce G; Chirtel S; Hammack TS
[Ad] Endereço:U.S. Food and Drug Administration, Center for Food Safety and Applied Nutrition, 5001 Campus Dr., College Park, MD 20740, USA. Electronic address: Andrew.Jacobson@fda.hhs.gov.
[Ti] Título:Relative effectiveness of selected preenrichment media for the detection of Salmonella from leafy green produce and herbs.
[So] Source:Food Microbiol;63:123-128, 2017 May.
[Is] ISSN:1095-9998
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Four buffered preenrichment media (BAX System MP Media (BAX)), Universal Preenrichment Broth (UPB), modified Buffered Peptone Water (mBPW), and Buffered Peptone Water (BPW)) were compared with lactose broth (LB) in the Bacteriological Analytical Manual's (BAM) Salmonella culture method for the analysis of 9 leafy green produce and herb types. Artificially contaminated test portions were pre-enriched in each medium and the results were analyzed statistically using Fisher's Exact 2-tailed F test (p < 0.05) with pairwise comparisons. There was no difference in recovery of Salmonella from curly parsley and basil among the five media (p > 0.05). UPB was consistently among the most effective media for recovery of Salmonella from the nine produce types; however, S. Typhimurium and S. Newport were isolated from cabbage more frequently with mBPW than with UPB (p < 0.05). Comparisons of the results among the preenrichment media from all experimental trials, with leafy green produce and herbs, demonstrate that Salmonella is more effectively detected and isolated using buffered enrichments than with the currently recommended LB (p < 0.05). There were no significant differences among the buffered preenrichments for the detection of Salmonella-positive test portions of the produce tested (BAX (160 Salmonella-positive test portions/480 test portions), UPB (176/480), mBPW (184/480), BPW (169/480), LB (128/480))(p > 0.05).
[Mh] Termos MeSH primário: Meios de Cultura/química
Ocimum basilicum/microbiologia
Petroselinum/microbiologia
Folhas de Planta/microbiologia
Salmonella/isolamento & purificação
Verduras/microbiologia
[Mh] Termos MeSH secundário: Carga Bacteriana
Técnicas Bacteriológicas/métodos
Tampões (Química)
Meios de Cultura/análise
Microbiologia de Alimentos
Lactose/metabolismo
Alface/microbiologia
Salmonella/crescimento & desenvolvimento
Spinacia oleracea/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Buffers); 0 (Culture Media); J2B2A4N98G (Lactose)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170307
[Lr] Data última revisão:
170307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170102
[St] Status:MEDLINE


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[PMID]:28012928
[Au] Autor:Abdellatief SA; Galal AA; Farouk SM; Abdel-Daim MM
[Ad] Endereço:Pharmacology Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Sharkia, Egypt.
[Ti] Título:Ameliorative effect of parsley oil on cisplatin-induced hepato-cardiotoxicity: A biochemical, histopathological, and immunohistochemical study.
[So] Source:Biomed Pharmacother;86:482-491, 2017 Feb.
[Is] ISSN:1950-6007
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Cisplatin (cis-diamminedichloroplatinum, CDDP) is an effective DNA alkylating agent used in the treatment of different types of tumors; however, its clinical use is associated with hepato-cardiotoxicity. The current study was designed to assess the potential protective effect of parsley oil (PO) against CDDP-induced hepato-cardiotoxicity. For this purpose, 25 adult male rats were assigned into five groups, each containing five animals. Group I (control) was administered saline solution. Group II was administered PO at a dosage of 0.42ml/kg BW. Group III were administered CDDP at a dosage of 5mg/kg BW. Group IV was administered PO in addition to CDDP. Group V was administered saline solution in addition to CDDP, after which they were administered PO for five days. Oral administration of either saline solution or PO was performed each day for 10days, while administration of CDDP was via a single intraperitoneal injection five days following the commencement of the experiment. The recorded results revealed that CDDP induced obvious hepatic and cardiac injuries that were indicated by biochemical, histopathological, and immunohistochemical alterations, including elevation of serum hepatic and cardiac injury markers as well as proinflammatory cytokines. Moreover, CDDP induced an increase in the level of hepatic and cardiac injury biomarkers, decreases in the activities of antioxidant enzymes, a decrease in GSH concentration, and an increase in MDA concentration. CDDP also induced histopathological hepatocellular and myocardial changes, and overexpression of p53 and COX-2 in hepatic and cardiac tissues. Administration of PO either as a preventative medicine or as treatment significantly improved all the observed deleterious effects induced by CDDP in rat liver and heart. Thus, it may be concluded that PO, with its antioxidant, anti-inflammatory, and antiapoptotic activities, can potentially be used in the treatment of CDDP-induced hepatic and cardiac injuries.
[Mh] Termos MeSH primário: Cardiotoxicidade/tratamento farmacológico
Doença Hepática Induzida por Substâncias e Drogas/tratamento farmacológico
Cisplatino/farmacologia
Traumatismos Cardíacos/tratamento farmacológico
Petroselinum/química
Óleos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Animais
Anti-Inflamatórios/química
Anti-Inflamatórios/farmacologia
Antioxidantes/metabolismo
Apoptose/efeitos dos fármacos
Cardiotoxicidade/metabolismo
Doença Hepática Induzida por Substâncias e Drogas/metabolismo
Glutationa/metabolismo
Coração/efeitos dos fármacos
Traumatismos Cardíacos/metabolismo
Inflamação/tratamento farmacológico
Inflamação/metabolismo
Fígado/efeitos dos fármacos
Fígado/metabolismo
Masculino
Estresse Oxidativo/efeitos dos fármacos
Óleos Vegetais/química
Ratos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Antioxidants); 0 (Plant Oils); GAN16C9B8O (Glutathione); Q20Q21Q62J (Cisplatin)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170208
[Lr] Data última revisão:
170208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161226
[St] Status:MEDLINE


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[PMID]:28011479
[Au] Autor:Schröder L; Koch J; Mahner S; Kost BP; Hofmann S; Jeschke U; Haumann J; Schmedt J; Richter DU
[Ad] Endereço:LMU Munich, Department of Obstetrics and Gynecology, Munich, Germany.
[Ti] Título:The Effects of Petroselinum Crispum on Estrogen Receptor-positive Benign and Malignant Mammary Cells (MCF12A/MCF7).
[So] Source:Anticancer Res;37(1):95-102, 2017 01.
[Is] ISSN:1791-7530
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Phytoestrogens have controversial effects on hormone-dependent tumors. Herein we investigated the effects of parsley root extract (PCE) on DNA synthesis performance, metabolic activity and cytotoxicity in malignant and benign breast cells. MATERIALS AND METHODS: The PCE was prepared and analyzed by mass spectrometry. MCF7 and MCF12A cells were incubated with various concentrations of PCE and analyzed for DNA synthesis performance, metabolic activity and cytotoxicity by BrdU proliferation, MTT and LDH assays, respectively. RESULTS: PCE was found to contain a substantial ratio of lignans. At a concentration range of 0.01 µg/ml-100 µg/ml the LDH assay analysis showed no significant cytotoxicity of PCE in both cell lines. However, at 500 µg/ml PCE's cytotoxicity was well over 70% of total cell population in both cell lines. According to the BrdU proliferation assay analysis, PCE demonstrated significant DNA synthesis inhibition of up to 80% at concentrations of 10, 50, 100 and 500 µg/ml in both cell lines. Based on the MTT assay analysis, only at a concentration of 500 µg/ml, PCE demonstrated a statistically significant inhibition of cellular metabolic activity of 63% in MCF7 and 75% in MCF12A of their respective normal capacity. CONCLUSION: PCE showed antiproliferative effects in MCF7 and MCF12A cells. Further investigation is required to determine whether this effect can be solely attributed to its phytoestrogens.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia
Neoplasias da Mama/tratamento farmacológico
Glândulas Mamárias Humanas/efeitos dos fármacos
Petroselinum
Fitoestrógenos/farmacologia
Extratos Vegetais/farmacologia
Receptores Estrogênicos/efeitos dos fármacos
[Mh] Termos MeSH secundário: Antineoplásicos Fitogênicos/isolamento & purificação
Neoplasias da Mama/metabolismo
Neoplasias da Mama/patologia
Proliferação Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Replicação do DNA/efeitos dos fármacos
Relação Dose-Resposta a Droga
Metabolismo Energético/efeitos dos fármacos
Feminino
Seres Humanos
Imuno-Histoquímica
Células MCF-7
Glândulas Mamárias Humanas/metabolismo
Glândulas Mamárias Humanas/patologia
Espectrometria de Massas
Petroselinum/química
Fitoestrógenos/isolamento & purificação
Fitoterapia
Extratos Vegetais/isolamento & purificação
Raízes de Plantas
Plantas Medicinais
Receptores Estrogênicos/metabolismo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Phytoestrogens); 0 (Plant Extracts); 0 (Receptors, Estrogen)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170130
[Lr] Data última revisão:
170130
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161225
[St] Status:MEDLINE


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[PMID]:27902522
[Au] Autor:Khosravan S; Alami A; Mohammadzadeh-Moghadam H; Ramezani V
[Ad] Endereço:Department of Community and Mental Health Nursing, Faculty of Nursing and Midwifery, Social Determinants of Health Research Centre (Dr Khosravan), Department of Health School of Public Health, Social Determinants of Health Research Center (Dr Alami), Department of Community Medicine, School of Medicine (Dr Mohammadzadeh-Moghadam), Student Research Committeen (Mr Ramezani); and Gonabad University of Medical Sciences, Gonabad, Iran.
[Ti] Título:The Effect of Topical Use of Petroselinum Crispum (Parsley) Versus That of Hydroquinone Cream on Reduction of Epidermal Melasma: A Randomized Clinical Trial.
[So] Source:Holist Nurs Pract;31(1):16-20, 2017 Jan/Feb.
[Is] ISSN:1550-5138
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Melasma disfigures the skin and thus influences people's self-image and self-concept. Therefore, melasma influences emotional and psychosocial health in addition to physical health. This clinical trial was performed to assess the effect of the topical use of Petroselinum crispum (parsley) on reduction of the severity of epidermal melasma.
[Mh] Termos MeSH primário: Fármacos Dermatológicos/uso terapêutico
Hidroquinonas/uso terapêutico
Melanose/tratamento farmacológico
Petroselinum/química
Extratos Vegetais/uso terapêutico
[Mh] Termos MeSH secundário: Adulto
Fármacos Dermatológicos/química
Feminino
Seres Humanos
Melanose/patologia
Meia-Idade
Extratos Vegetais/química
Resultado do Tratamento
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Dermatologic Agents); 0 (Hydroquinones); 0 (Plant Extracts); XV74C1N1AE (hydroquinone)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170227
[Lr] Data última revisão:
170227
[Sb] Subgrupo de revista:N
[Da] Data de entrada para processamento:161201
[St] Status:MEDLINE



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