Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.075.937 [Categoria DeCS]
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  1 / 22 MEDLINE  
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[PMID]:28111971
[Au] Autor:Neffati N; Aloui Z; Karoui H; Guizani I; Boussaid M; Zaouali Y
[Ad] Endereço:a Laboratoire d'Epidémiologie Moléculaire et Pathologie Expérimentale Appliquée aux Maladies Infectieuses (LR11IPT04) , Institut Pasteur de Tunis, Université de Tunis El Manar , Tunis , Tunisia.
[Ti] Título:Phytochemical composition and antioxidant activity of medicinal plants collected from the Tunisian flora.
[So] Source:Nat Prod Res;31(13):1583-1588, 2017 Jul.
[Is] ISSN:1478-6427
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Polyphenols, flavonoids and condensed tannins contents, as well as the antioxidant activity of the ethanolic and aqueous extracts obtained from aerial parts of 10 wild Tunisian plants, have been determined. Extracts showed appreciable levels of polyphenols and flavonoids, which reached 215.16 mg GAE g DW in Lavandula stoechas ethanolic extract, and 49.12 mg RE g DW in Thapsia garganica aqueous extract. The majority of tested extracts exhibited low total condensed tannins content, except for Rhus tripartitum and Periploca laevigata. The antioxidant activity tests showed great activity, especially for R. tripartitum and Lavandula multifida (IC = 5.16 and 5.1 µg mL , respectively). Canonical Correspondence Analysis revealed clear groupings of species according to the solvent used.
[Mh] Termos MeSH primário: Antioxidantes
Flavonoides/análise
Compostos Fitoquímicos
Extratos Vegetais/química
Plantas Medicinais/química
Polifenóis/análise
[Mh] Termos MeSH secundário: Antioxidantes/química
Etanol
Lavandula/química
Fenóis/análise
Proantocianidinas/análise
Rhus/química
Thapsia/química
Tunísia
Água
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Flavonoids); 0 (Phenols); 0 (Phytochemicals); 0 (Plant Extracts); 0 (Polyphenols); 0 (Proanthocyanidins); 059QF0KO0R (Water); 3K9958V90M (Ethanol)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170124
[St] Status:MEDLINE
[do] DOI:10.1080/14786419.2017.1280490


  2 / 22 MEDLINE  
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[PMID]:26540577
[Au] Autor:Casiglia S; Riccobono L; Bruno M; Rosselli S; Senatore F; Senatore F
[Ad] Endereço:a Department STEBICEF , University of Palermo , Palermo , Italy.
[Ti] Título:Chemical composition of the essential oil from Thapsia garganica L. (Apiaceae) grown wild in Sicily and its antimicrobial activity.
[So] Source:Nat Prod Res;30(9):1042-52, 2016.
[Is] ISSN:1478-6427
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In this study, the chemical composition of the essential oil from flowers and leaves of Thapsia garganica L. collected in Sicily was evaluated by GC and GC-MS. The main components of T. garganica flower oil (T.f.) were chamazulene (58.3%), humulene oxide II (9.0%), tricosane (8.2%) and pentacosane (8.2%). Also the oil from leaves (T.l.) was characterised by high content of chamazulene (49.2%). Other abundant metabolites were 1,4-dimethylazulene (18.5%), (E)-phytol (6.3%) and neophytadiene (5.1%). The comparison with other studied oils of genus Thapsia is discussed. Antimicrobial activity against several micro-organisms, including some ones infesting historical art craft, was also determined.
[Mh] Termos MeSH primário: Anti-Infecciosos/farmacologia
Óleos Voláteis/química
Óleos Voláteis/farmacologia
Thapsia/química
[Mh] Termos MeSH secundário: Antibacterianos/farmacologia
Anti-Infecciosos/isolamento & purificação
Antifúngicos/farmacologia
Bactérias/efeitos dos fármacos
Flores/química
Fungos/efeitos dos fármacos
Cromatografia Gasosa-Espectrometria de Massas
Testes de Sensibilidade Microbiana
Folhas de Planta/química
Sicília
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Anti-Infective Agents); 0 (Antifungal Agents); 0 (Oils, Volatile)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151106
[St] Status:MEDLINE
[do] DOI:10.1080/14786419.2015.1104676


  3 / 22 MEDLINE  
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[PMID]:26078214
[Au] Autor:Doan NT; Crestey F; Olsen CE; Christensen SB
[Ad] Endereço:†Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, 2100 Copenhagen Ø, Denmark.
[Ti] Título:Chemo- and Regioselective Functionalization of Nortrilobolide: Application for Semisynthesis of the Natural Product 2-Acetoxytrilobolide.
[So] Source:J Nat Prod;78(6):1406-14, 2015 Jun 26.
[Is] ISSN:1520-6025
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The difference in reactivity of the hexaoxygenated natural product thapsigargin (1) and the pentaoxygenated nortrilobolide (3) was compared in order to develop a chemo- and regioselective method for the conversion of nortrilobolide (3) into the natural product 2-acetoxytrilobolide (4). For the first time, a stereoselective synthesis of 2-acetoxytrilobolide (4) is described, which involves two key reactions: the first chemical step was a one-pot substitution-oxidation reaction of an allylic ester into its corresponding α,ß-unsaturated ketone. The second process consisted of a stereoselective α'-acyloxylation of the key intermediate α,ß-unsaturated ketone to afford its corresponding acetoxyketone, which was converted into 2-acetoxytrilobolide (4) in a few steps. This innovative approach would allow the synthesis of a broad library of novel and valuable penta- and hexaoxygenated guaianolides as potential anticancer agents.
[Mh] Termos MeSH primário: Antineoplásicos/síntese química
Azulenos/química
Azulenos/síntese química
Sesquiterpenos de Guaiano/química
Sesquiterpenos de Guaiano/síntese química
Thapsia/química
[Mh] Termos MeSH secundário: Antineoplásicos/química
Antineoplásicos/farmacologia
Azulenos/farmacologia
Técnicas de Química Combinatória
Estrutura Molecular
Ressonância Magnética Nuclear Biomolecular
Sesquiterpenos de Guaiano/farmacologia
Estereoisomerismo
Tapsigargina/síntese química
Tapsigargina/química
Tapsigargina/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (2-acetoxytrilobolide); 0 (Antineoplastic Agents); 0 (Azulenes); 0 (Sesquiterpenes, Guaiane); 136051-63-3 (nortrilobolide); 67526-95-8 (Thapsigargin)
[Em] Mês de entrada:1509
[Cu] Atualização por classe:150626
[Lr] Data última revisão:
150626
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150617
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jnatprod.5b00333


  4 / 22 MEDLINE  
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[PMID]:25879507
[Au] Autor:Hassen I; M'Rabet Y; Belgacem C; Kesraoui O; Casabianca H; Hosni K
[Ad] Endereço:Laboratoire des Méthodes et Techniques Analytiques, Institut National de Recherche et d'Analyse Physico-Chimique (INRAP), Biotechpole de Sidi Thabet, 2020, Tunisia.
[Ti] Título:Chemodiversity of volatile oils in Thapsia garganica L. (Apiaceae).
[So] Source:Chem Biodivers;12(4):637-51, 2015 Apr.
[Is] ISSN:1612-1880
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The chemical composition of the volatile oils obtained from the roots, leaves, flowers, and stems of Thapsia garganica of Tunisian origin was investigated by GC-FID and GC/MS analyses. Sesquiterpene hydrocarbons and oxygenated monoterpenes were predominant in the oils of all plant parts. Bicyclogermacrene (21.59-35.09%) was the main component in the former compound class, whereas geranial (3.31-14.84%) and linalool (0.81-10.9%) were the most prominent ones in the latter compound class. Principal-component (PCA) and hierarchical-cluster (HCA) analyses revealed some common constituents, but also significant variability amongst the oils of the different plant parts. This organ-specific oil composition was discussed in relation to their biological and ecological functions. For the evaluation of the intraspecific chemical variability in T. garganica, the composition of the flower volatile oils from four wild populations was investigated. Bicyclogermacrene, linalool, and geranial were predominant in the oils of three populations, whereas epicubenol, ß-sesquiphellandrene, and cadina-1,4-diene were the most prominent components of the oil of one population. PCA and HCA allowed the separation of the flower oils into three distinct groups, however, no relationship was found between the volatile-oil composition and the geographical distribution and pedoclimatic conditions of the studied populations.
[Mh] Termos MeSH primário: Óleos Voláteis/química
Thapsia/química
[Mh] Termos MeSH secundário: Biodiversidade
Análise por Conglomerados
Flores/química
Cromatografia Gasosa-Espectrometria de Massas
Monoterpenos/análise
Naftalenos/análise
Óleos Voláteis/análise
Folhas de Planta/química
Óleos Vegetais/química
Raízes de Plantas/química
Caules de Planta/química
Análise de Componente Principal
Sesquiterpenos/análise
Sesquiterpenos de Germacrano/análise
Tunísia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Monoterpenes); 0 (Naphthalenes); 0 (Oils, Volatile); 0 (Plant Oils); 0 (Sesquiterpenes); 0 (Sesquiterpenes, Germacrane); 0 (epicubenol); D81QY6I88E (linalool); T7EU0O9VPP (citral)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:150417
[Lr] Data última revisão:
150417
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150417
[St] Status:MEDLINE
[do] DOI:10.1002/cbdv.201400218


  5 / 22 MEDLINE  
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[PMID]:25856061
[Au] Autor:Andersen TB; López CQ; Manczak T; Martinez K; Simonsen HT
[Ad] Endereço:Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg, Denmark. trba@plen.ku.dk.
[Ti] Título:Thapsigargin--from Thapsia L. to mipsagargin.
[So] Source:Molecules;20(4):6113-27, 2015 Apr 08.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The sesquiterpene lactone thapsigargin is found in the plant Thapsia garganica L., and is one of the major constituents of the roots and fruits of this Mediterranean species. In 1978, the first pharmacological effects of thapsigargin were established and the full structure was elucidated in 1985. Shortly after, the overall mechanism of the Sarco-endoplasmic reticulum Ca2+-ATPase (SERCA) inhibition that leads to apoptosis was discovered. Thapsigargin has a potent antagonistic effect on the SERCA and is widely used to study Ca2+-signaling. The effect on SERCA has also been utilized in the treatment of solid tumors. A prodrug has been designed to target the blood vessels of cancer cells; the death of these blood vessels then leads to tumor necrosis. The first clinical trials of this drug were initiated in 2008, and the potent drug is expected to enter the market in the near future under the generic name Mipsagargin (G-202). This review will describe the discovery of the new drug, the on-going elucidation of the biosynthesis of thapsigargin in the plant and attempts to supply the global market with a novel potent anti-cancer drug.
[Mh] Termos MeSH primário: Thapsia/química
Thapsia/fisiologia
Tapsigargina/metabolismo
Tapsigargina/farmacologia
[Mh] Termos MeSH secundário: Fermentação
Thapsia/classificação
Tapsigargina/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Nm] Nome de substância:
67526-95-8 (Thapsigargin)
[Em] Mês de entrada:1601
[Cu] Atualização por classe:150410
[Lr] Data última revisão:
150410
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150410
[St] Status:MEDLINE
[do] DOI:10.3390/molecules20046113


  6 / 22 MEDLINE  
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[PMID]:25581992
[Au] Autor:Keller V
[Ad] Endereço:University of Oregon, Eugene, USA. vkeller@uoregon.edu
[Ti] Título:Nero and the last stalk of Silphion: collecting extinct nature in early modern Europe.
[So] Source:Early Sci Med;19(5):424-47, 2014.
[Is] ISSN:1383-7427
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Many studies of early modern natural history focus upon observational, empirical techniques. Early moderns also contended with entities which could no longer be observed because they no longer existed. Although it is often assumed that extinction only emerged as a concept in the eighteenth century, the concept of natural loss appeared, often unproblematically, in areas outside natural philosophy. A survey of discussions of the extinct plant silphion across Europe in the sixteenth and seventeenth centuries shows that the possibility of natural loss was well aired. Paper technologies for collecting extinct nature ran parallel to investigations of newly found nature, and thus can place the latter in a new light. Although ideas of natural mutability often drew on ideas of historical or political change rather than philosophical concepts of natural constancy, techniques developed for extinct nature, such as the list of lost things, remained influential for the research agendas of naturalists.
[Mh] Termos MeSH primário: Extinção Biológica
Ferula/fisiologia
História Natural/história
Thapsia/fisiologia
[Mh] Termos MeSH secundário: Asteraceae
Europa (Continente)
História do Século XVI
História do Século XVII
Líbia
Plantas Medicinais/fisiologia
[Pt] Tipo de publicação:HISTORICAL ARTICLE; JOURNAL ARTICLE
[Em] Mês de entrada:1502
[Cu] Atualização por classe:161013
[Lr] Data última revisão:
161013
[Sb] Subgrupo de revista:QIS
[Da] Data de entrada para processamento:150114
[St] Status:MEDLINE


  7 / 22 MEDLINE  
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[PMID]:25433927
[Au] Autor:Mohamed M; Soua Y; Njim L; Hammemi S; Youssef M; Akkari H; Belhadjali H; Zili J
[Ad] Endereço:Service de dermatologie, CHU de Monastir, R4, N 112, 5000 Monastir, Tunisie. Electronic address: mariemmohamed79@yahoo.fr.
[Ti] Título:[Acute localized exanthematous pustulosis on the face: 6 cases in Tunisia].
[Ti] Título:Pustulose exanthématique aiguë localisée de la face : six cas tunisiens..
[So] Source:Ann Dermatol Venereol;141(12):756-64, 2014 Dec.
[Is] ISSN:0151-9638
[Cp] País de publicação:France
[La] Idioma:fre
[Ab] Resumo:BACKGROUND: Acute localized exanthematous pustulosis (ALEP) is a rare and localized variant of acute generalized exanthematous pustulosis (AGEP). Only 15 cases of ALEP have been reported to date in the literature, with all cases following drug administration. We report 6 paediatric cases of ALEP occurring in springtime, with no associated drug administration in any case. PATIENTS AND METHODS: Over the last three years (2011, 2012 and 2013), we observed 6 cases of ALEP in 6 Tunisian children aged between 9 and 14 years. All cases were observed during the spring months. Diagnosis of ALEP was based in all cases on the EuroSCAR criteria and on the definition of ALEP proposed by Prange et al. A drug-related aetiology was ruled out in all cases, with exposure to a specific planned plant (Thapsia garganica) being retained as an aetiological factor in one case. DISCUSSION: Drug administration is the most frequent though not the sole cause of ALEP. The seasonal nature of this dermatosis may suggest other causes, mainly viral infection, plant contact or airborne allergens.
[Mh] Termos MeSH primário: Pustulose Exantematosa Aguda Generalizada/diagnóstico
Dermatoses Faciais/diagnóstico
[Mh] Termos MeSH secundário: Pustulose Exantematosa Aguda Generalizada/tratamento farmacológico
Pustulose Exantematosa Aguda Generalizada/epidemiologia
Pustulose Exantematosa Aguda Generalizada/etiologia
Pustulose Exantematosa Aguda Generalizada/patologia
Adolescente
Corticosteroides/uso terapêutico
Antibacterianos/uso terapêutico
Biópsia
Criança
Dermatoses Faciais/tratamento farmacológico
Dermatoses Faciais/epidemiologia
Dermatoses Faciais/etiologia
Dermatoses Faciais/patologia
Feminino
Flores/efeitos adversos
Seres Humanos
Leucócitos/patologia
Masculino
Estações do Ano
Thapsia/efeitos adversos
Tunísia/epidemiologia
[Pt] Tipo de publicação:CASE REPORTS; ENGLISH ABSTRACT; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adrenal Cortex Hormones); 0 (Anti-Bacterial Agents)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:141201
[Lr] Data última revisão:
141201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141201
[St] Status:MEDLINE


  8 / 22 MEDLINE  
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[PMID]:23698765
[Au] Autor:Drew DP; Dueholm B; Weitzel C; Zhang Y; Sensen CW; Simonsen HT
[Ad] Endereço:Department of Plant and Environmental Sciences, Faculty of Sciences, University of Copenhagen, Frederiksberg DK-1871, Denmark. hts@life.ku.dk.
[Ti] Título:Transcriptome analysis of Thapsia laciniata Rouy provides insights into terpenoid biosynthesis and diversity in Apiaceae.
[So] Source:Int J Mol Sci;14(5):9080-98, 2013 Apr 25.
[Is] ISSN:1422-0067
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Thapsia laciniata Rouy (Apiaceae) produces irregular and regular sesquiterpenoids with thapsane and guaiene carbon skeletons, as found in other Apiaceae species. A transcriptomic analysis utilizing Illumina next-generation sequencing enabled the identification of novel genes involved in the biosynthesis of terpenoids in Thapsia. From 66.78 million HQ paired-end reads obtained from T. laciniata roots, 64.58 million were assembled into 76,565 contigs (N50: 1261 bp). Seventeen contigs were annotated as terpene synthases and five of these were predicted to be sesquiterpene synthases. Of the 67 contigs annotated as cytochromes P450, 18 of these are part of the CYP71 clade that primarily performs hydroxylations of specialized metabolites. Three contigs annotated as aldehyde dehydrogenases grouped phylogenetically with the characterized ALDH1 from Artemisia annua and three contigs annotated as alcohol dehydrogenases grouped with the recently described ADH1 from A. annua. ALDH1 and ADH1 were characterized as part of the artemisinin biosynthesis. We have produced a comprehensive EST dataset for T. laciniata roots, which contains a large sample of the T. laciniata transcriptome. These transcriptome data provide the foundation for future research into the molecular basis for terpenoid biosynthesis in Thapsia and on the evolution of terpenoids in Apiaceae.
[Mh] Termos MeSH primário: Biodiversidade
Perfilação da Expressão Gênica/métodos
Terpenos/metabolismo
Thapsia/genética
[Mh] Termos MeSH secundário: Aldeído Desidrogenase/genética
Aldeído Desidrogenase/metabolismo
Alquil e Aril Transferases
Sequência de Aminoácidos
Ciclo do Ácido Cítrico
Sistema Enzimático do Citocromo P-450/genética
Sistema Enzimático do Citocromo P-450/metabolismo
Eletroforese em Gel de Ágar
Regulação da Expressão Gênica de Plantas
Genes de Plantas
Geraniltranstransferase/metabolismo
Dados de Sequência Molecular
Família Multigênica
Filogenia
Proteínas de Plantas/química
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
Reação em Cadeia da Polimerase
Alinhamento de Sequência
Análise de Sequência de RNA
Sesquiterpenos/metabolismo
Terpenos/química
Thapsia/enzimologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Plant Proteins); 0 (Sesquiterpenes); 0 (Terpenes); 9035-51-2 (Cytochrome P-450 Enzyme System); EC 1.2.1.3 (Aldehyde Dehydrogenase); EC 2.5.- (Alkyl and Aryl Transferases); EC 2.5.1.- (terpene synthase); EC 2.5.1.10 (Geranyltranstransferase)
[Em] Mês de entrada:1509
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130524
[St] Status:MEDLINE
[do] DOI:10.3390/ijms14059080


  9 / 22 MEDLINE  
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[PMID]:23681694
[Au] Autor:Nelson HM; Gordon JR; Virgil SC; Stoltz BM
[Ad] Endereço:Warren and Katharine Schlinger Laboratory for Chemistry and Chemical Engineering, Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 E. California Boulevard, MC 101-20, Pasadena, CA 91125, USA.
[Ti] Título:Total syntheses of (-)-transtaganolide A, (+)-transtaganolide B, (+)-transtaganolide C, and (-)-transtaganolide D and biosynthetic implications.
[So] Source:Angew Chem Int Ed Engl;52(26):6699-703, 2013 Jun 24.
[Is] ISSN:1521-3773
[Cp] País de publicação:Germany
[La] Idioma:eng
[Mh] Termos MeSH primário: Artemisininas/síntese química
Sesquiterpenos/síntese química
[Mh] Termos MeSH secundário: Desenho de Drogas
Estrutura Molecular
Extratos Vegetais/química
Pironas/química
Temperatura Ambiente
Thapsia/metabolismo
Tapsigargina/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Artemisinins); 0 (Plant Extracts); 0 (Pyrones); 0 (Sesquiterpenes); 0 (basiliolide B); 0 (transtaganolide C); 0 (transtaganolide D); 67526-95-8 (Thapsigargin)
[Em] Mês de entrada:1401
[Cu] Atualização por classe:161019
[Lr] Data última revisão:
161019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130518
[St] Status:MEDLINE
[do] DOI:10.1002/anie.201301212


  10 / 22 MEDLINE  
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[PMID]:23537742
[Au] Autor:Ollivier A; Grougnet R; Cachet X; Meriane D; Ardisson J; Boutefnouchet S; Deguin B
[Ad] Endereço:Laboratoire de Pharmacognosie, U.M.R./C.N.R.S. 8638, Faculté des Sciences Pharmaceutiques et Biologiques, Université Paris Descartes, Sorbonne Paris Cité, 4 Avenue de l'Observatoire, F-75006 Paris, France.
[Ti] Título:Large scale purification of the SERCA inhibitor thapsigargin from Thapsia garganica L. roots using centrifugal partition chromatography.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;926:16-20, 2013 May 01.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Thapsigargin (Tg) is a selective and irreversible inhibitor of the sarcoplasmic/endoplasmic reticulum calcium ATPase (SERCA)-dependent pump at subnanomolecular concentrations. As such, it has become a powerful tool in the study of Ca(2+) signaling pathway. Purification of Tg from Thapsia species requires repeated chromatographic steps with normal-phase alumina or silica and reverse phase chromatography. We thus developed an innovative procedure coupling high pressure automatized extraction with centrifugal partition chromatography allowing a fast and safe large-scale isolation of highly pure Tg, in two steps from Thapsia garganica L. roots. Comparison of influence of extraction procedures, storage conditions and harvesting areas on Tg content in different Algerian specimens of Thapsia garganica L. roots has been precised by mean of HPLC quantification procedure. Highest Tg content were found in the fresh material of the sample from Setif area.
[Mh] Termos MeSH primário: Centrifugação/métodos
Cromatografia Líquida de Alta Pressão/métodos
Inibidores Enzimáticos/farmacologia
Raízes de Plantas/química
ATPases Transportadoras de Cálcio do Retículo Sarcoplasmático/antagonistas & inibidores
Thapsia/química
[Mh] Termos MeSH secundário: Inibidores Enzimáticos/isolamento & purificação
Espectroscopia de Ressonância Magnética
Espectrofotometria Ultravioleta
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Enzyme Inhibitors); EC 3.6.3.8 (Sarcoplasmic Reticulum Calcium-Transporting ATPases)
[Em] Mês de entrada:1308
[Cu] Atualização por classe:130415
[Lr] Data última revisão:
130415
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130330
[St] Status:MEDLINE



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