Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.087.412 [Categoria DeCS]
Referências encontradas : 128 [refinar]
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[PMID]:28771546
[Au] Autor:Sun H; Li F; Xu Z; Sun M; Cong H; Qiao F; Zhong X
[Ad] Endereço:Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture / Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, China.
[Ti] Título:De novo leaf and root transcriptome analysis to identify putative genes involved in triterpenoid saponins biosynthesis in Hedera helix L.
[So] Source:PLoS One;12(8):e0182243, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Hedera helix L. is an important traditional medicinal plant in Europe. The main active components are triterpenoid saponins, but none of the potential enzymes involved in triterpenoid saponins biosynthesis have been discovered and annotated. Here is reported the first study of global transcriptome analyses using the Illumina HiSeq™ 2500 platform for H. helix. In total, over 24 million clean reads were produced and 96,333 unigenes were assembled, with an average length of 1385 nt; more than 79,085 unigenes had at least one significant match to an existing gene model. Differentially Expressed Gene analysis identified 6,222 and 7,012 unigenes which were expressed either higher or lower in leaf samples when compared with roots. After functional annotation and classification, two pathways and 410 unigenes related to triterpenoid saponins biosynthesis were discovered. The accuracy of these de novo sequences was validated by RT-qPCR analysis and a RACE clone. These data will enrich our knowledge of triterpenoid saponin biosynthesis and provide a theoretical foundation for molecular research on H. helix.
[Mh] Termos MeSH primário: Hedera/genética
Folhas de Planta/genética
Raízes de Plantas/genética
Saponinas/biossíntese
Saponinas/genética
Transcriptoma
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
DNA Complementar/química
DNA Complementar/metabolismo
Perfilação da Expressão Gênica
Hedera/metabolismo
Hidroximetilglutaril-CoA-Redutases NADP-Dependentes/química
Hidroximetilglutaril-CoA-Redutases NADP-Dependentes/genética
Hidroximetilglutaril-CoA-Redutases NADP-Dependentes/metabolismo
Folhas de Planta/metabolismo
Proteínas de Plantas/química
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
Raízes de Plantas/metabolismo
RNA de Plantas/isolamento & purificação
RNA de Plantas/metabolismo
Reação em Cadeia da Polimerase em Tempo Real
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Complementary); 0 (Plant Proteins); 0 (RNA, Plant); 0 (Saponins); EC 1.1.1.34 (Hydroxymethylglutaryl-CoA-Reductases, NADP-dependent)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170804
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182243


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[PMID]:28641575
[Au] Autor:Valcárcel V; Guzmán B; Medina NG; Vargas P; Wen J
[Ad] Endereço:Department of Biology (Botany), Universidad Autónoma de Madrid, Madrid, Spain. virginia.valcarcel@uam.es.
[Ti] Título:Phylogenetic and paleobotanical evidence for late Miocene diversification of the Tertiary subtropical lineage of ivies (Hedera L., Araliaceae).
[So] Source:BMC Evol Biol;17(1):146, 2017 Jun 22.
[Is] ISSN:1471-2148
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Hedera (ivies) is one of the few temperate genera of the primarily tropical Asian Palmate group of the Araliaceae, which extends its range out of Asia to Europe and the Mediterranean basin. Phylogenetic and phylogeographic results suggested Asia as the center of origin and the western Mediterranean region as one of the secondary centers of diversification. The bird-dispersed fleshy fruits of ivies suggest frequent dispersal over long distances (e.g. Macaronesian archipelagos), although reducing the impact of geographic barriers to gene flow in mainland species. Genetic isolation associated with geographic barriers and independent polyploidization events have been postulated as the main driving forces of diversification. In this study we aim to evaluate past and present diversification patterns in Hedera within a geographic and temporal framework to clarify the biogeographic history of the genus. RESULTS: Phylogenetic (biogeographic, time divergence and diversification) and phylogeographic (coalescence) analyses using four DNA regions (nrITS, trnH-psbA, trnT-trnL, rpl32) revealed a complex spatial pattern of lineage divergence. Scarce geographic limitation to gene flow and limited diversification are observed during the early-mid Miocene, followed by a diversification rate increase related to geographic divergence from the Tortonian/Messinian. Genetic and palaeobotanical evidence points the origin of the Hedera clade in Asia, followed by a gradual E-W Asian extinction and the progressive E-W Mediterranean colonization. The temporal framework for the E Asia - W Mediterranean westward colonization herein reported is congruent with the fossil record. Subsequent range expansion in Europe and back colonization to Asia is also inferred. Uneven diversification among geographic areas occurred from the Tortonian/Messinian onwards with limited diversification in the newly colonized European and Asian regions. Eastern and western Mediterranean regions acted as refugia for Miocene and post-Miocene lineages, with a similar role as consecutive centers of centrifugal dispersal (including islands) and speciation. CONCLUSIONS: The Miocene Asian extinction and European survival of Hedera question the general pattern of Tertiary regional extinction of temperate angiosperms in Europe while they survived in Asia. The Tortonian/Messinian diversification increase of ivies in the Mediterranean challenges the idea that this aridity period was responsible for the extinction of the Mediterranean subtropical Tertiary flora. Differential responses of Hedera to geographic barriers throughout its evolutionary history, linked to spatial isolation related to historical geologic and climatic constraints may have shaped diversification of ivies in concert with recurrent polyploidy.
[Mh] Termos MeSH primário: Hedera/classificação
Hedera/genética
[Mh] Termos MeSH secundário: Ásia
Evolução Biológica
Ecossistema
Europa (Continente)
Fósseis
Especiação Genética
Filogenia
Filogeografia
Poliploidia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170624
[St] Status:MEDLINE
[do] DOI:10.1186/s12862-017-0984-1


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[PMID]:28605982
[Au] Autor:Rosca-Casian O; Mircea C; Vlase L; Gheldiu AM; Teuca DT; Pârvu M
[Ad] Endereço:"A. Borza" Botanical Garden, "Babes-Bolyai" University , 42 Republicii Street, 400015 Cluj-Napoca , Romania.
[Ti] Título:Chemical composition and antifungal activity of Hedera helix leaf ethanolic extract.
[So] Source:Acta Biol Hung;68(2):196-207, 2017 Jun.
[Is] ISSN:0236-5383
[Cp] País de publicação:Hungary
[La] Idioma:eng
[Ab] Resumo:The 50% ethanol extract obtained from Hedera helix leaves was investigated regarding the presence and quantity of polyphenols, sterols and in vitro antifungal activity against phytopathogenic fungi. The chemical analysis revealed the presence of rutin, quercetin and kaempferol in the non-hydrolysed sample and quercetin and kaempferol in the hydrolysed sample and stigmasterol in the ivy leaf extract (nonhydrolysed sample). The antifungal activity against phytopathogenic fungi (Aspergillus niger, Botrytis cinerea, B. tulipae, Fusarium oxysporum f. sp. tulipae, Penicillium gladioli, and Sclerotinia sclerotiorum) was assessed using an agar dilution assay. The results are expressed as the minimum inhibitory concentration (MIC = 10-14%) and were compared to a synthetic antifungal drug - fluconazole (MIC = 8-30%). This report presents the first screening of the antifungal activity of the ivy leaf extract on these plant pathogenic fungi species, aiming to use the ivy leaf extract for controlling different diseases of vegetables and ornamental plants, in addition to human disorders.
[Mh] Termos MeSH primário: Antifúngicos/farmacologia
Fungos/crescimento & desenvolvimento
Hedera/química
Extratos Vegetais/farmacologia
Folhas de Planta/química
[Mh] Termos MeSH secundário: Antifúngicos/química
Relação Dose-Resposta a Droga
Etanol/química
Extratos Vegetais/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Plant Extracts); 3K9958V90M (Ethanol)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170814
[Lr] Data última revisão:
170814
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170614
[St] Status:MEDLINE
[do] DOI:10.1556/018.68.2017.2.7


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[PMID]:27592174
[Au] Autor:Zhang D; Zhang QS; Yang XQ; Sheng ZT; Nan GN
[Ad] Endereço:Ocean School, Yantai University, Yantai, 264005, PR China.
[Ti] Título:The alternation between PSII and PSI in ivy (Hedera nepalensis) demonstrated by in vivo chlorophyll a fluorescence and modulated 820 nm reflection.
[So] Source:Plant Physiol Biochem;108:499-506, 2016 Nov.
[Is] ISSN:1873-2690
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:To examine the coordination between photosystem II (PSII) and photosystem I (PSI) in response to varying environmental conditions, both diurnal fluctuations and seasonal variability of photosynthetic electron transport activity in ivy (Hedera nepalensis, Araliaceae) were investigated: by measuring prompt fluorescence, delayed fluorescence (DF) and modulated reflection of 820 nm light (MR). During diurnal fluctuations, the PSII electron donor side was damaged, as evidenced by decreases of the fast amplitude of DF decay kinetics at I , although there was no significant change in relative variable fluorescence at K-step to amplitude of F - F . Decreases in the maximum photochemical efficiency (i.e., PSII photoinactivation) were accompanied by an increased maximum decrease in the slope of MR/MR (i.e., PSI photoactivation). Subsequently, PSII recovery and PSI relaxation occurred in the afternoon. Throughout the season, alternations between PSII and PSI were also suggested by the down-regulation of PSII and the up-regulation of PSI from summer to winter. Significant negative linear correlations between the activity of PSII and PSI across both diurnal fluctuations and seasonal variability were verified by correlation analyses. In addition, PSI was active throughout the year, suggesting PSI is independent from high temperatures. High PSI activity may maintain the functional integrity of the photosynthetic apparatus in overwintering ivy. The alternation between PSII and PSI activity may regulate the distribution of excitation energy between the two photosystems and balance the redox state of the electron transport change, thereby enabling ivy to respond to varying environmental conditions.
[Mh] Termos MeSH primário: Clorofila/metabolismo
Hedera/metabolismo
Complexo de Proteína do Fotossistema I/metabolismo
Complexo de Proteína do Fotossistema II/metabolismo
[Mh] Termos MeSH secundário: Ritmo Circadiano
Transporte de Elétrons
Fluorescência
Hedera/química
Hedera/fisiologia
Fotossíntese
Proteínas de Plantas/metabolismo
Estações do Ano
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Photosystem I Protein Complex); 0 (Photosystem II Protein Complex); 0 (Plant Proteins); 1406-65-1 (Chlorophyll); YF5Q9EJC8Y (chlorophyll a)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170813
[Lr] Data última revisão:
170813
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160905
[St] Status:MEDLINE


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[PMID]:27474936
[Au] Autor:Sun HP; Li F; Ruan QM; Zhong XH
[Ad] Endereço:Horticulture & Landscape College, Hunan Agricultural University, Changsha, Hunan 410128, China.
[Ti] Título:Identification and validation of reference genes for quantitative real-time PCR studies in Hedera helix L.
[So] Source:Plant Physiol Biochem;108:286-294, 2016 Nov.
[Is] ISSN:1873-2690
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Reference gene evaluation and selection are necessary steps in gene expression analysis, especially in new plant varieties, through reverse transcription quantitative real-time PCR (RT-qPCR). Hedera helix L. is an important traditional medicinal plant recorded in European Pharmacopoeia. Research on gene expression in H. helix has not been widely explored, and no RT-qPCR studies have been reported. Thus, it is important and necessary to identify and validate suitable reference genes to for normalizing RT-qPCR results. In our study, 14 candidate protein-coding reference genes were selected. Their expression stability in five tissues (root, stem, leaf, petiole and shoot tip) and under seven abiotic stress conditions (cold, heat, drought, salinity, UV-C irradiation, abscisic acid and methyl jasmonate) were evaluated using geNorm and NormFinder. This study is the first to evaluate the stability of reference genes in H. helix. The results show that different reference genes should be chosen for normalization on the basis of various experimental conditions. F-box was more stable than the other selected genes under all analysis conditions except ABA treatment; 40S was the most stable reference gene under ABA treatment; in contrast, EXP and UBQ were the most unstable reference genes. The expressions of HhSE and Hhß-AS, which are two genes related to the biosynthetic pathway of triterpenoid saponins, were also examined for reference genes in different tissues and under various cold stress conditions. The validation results confirmed the applicability and accuracy of reference genes. Additionally, this study provides a basis for the accurate and widespread use of RT-qPCR in selecting genes from the genome of H. helix.
[Mh] Termos MeSH primário: Genes de Plantas
Hedera/genética
Reação em Cadeia da Polimerase em Tempo Real/normas
[Mh] Termos MeSH secundário: Ácido Abscísico/farmacologia
Acetatos/farmacologia
Ciclopentanos/farmacologia
Primers do DNA
Secas
Perfilação da Expressão Gênica/métodos
Perfilação da Expressão Gênica/normas
Hedera/efeitos dos fármacos
Hedera/efeitos da radiação
Oxilipinas/farmacologia
Reação em Cadeia da Polimerase em Tempo Real/métodos
Padrões de Referência
Salinidade
Estresse Fisiológico/genética
Raios Ultravioleta
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Acetates); 0 (Cyclopentanes); 0 (DNA Primers); 0 (Oxylipins); 72S9A8J5GW (Abscisic Acid); 900N171A0F (methyl jasmonate)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170813
[Lr] Data última revisão:
170813
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160731
[St] Status:MEDLINE


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[PMID]:27411171
[Au] Autor:Rehman SU; Choi MS; Kim IS; Kim SH; Yoo HH
[Ad] Endereço:Institute of Pharmaceutical Science and Technology and College of Pharmacy, Hanyang University, Ansan, Gyeonggi-do 426-791, Republic of Korea.
[Ti] Título:An ultra-high-performance liquid chromatography-tandem mass spectrometric method for the determination of hederacoside C, a drug candidate for respiratory disorder, in rat plasma.
[So] Source:J Pharm Biomed Anal;129:90-95, 2016 Sep 10.
[Is] ISSN:1873-264X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Hederacoside C is a principal bioactive pharmaceutical ingredient of Hedera helix leaf extracts. H. helix extracts have long been used in folk medicine for the treatment of respiratory disorders. Currently, hederacoside C is investigated as a promising candidate for the treatment of respiratory diseases. In this study, an accurate, sensitive, rapid, and reliable bioanalytical method was developed for the determination of hederacoside C in rat plasma using ultra high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). For sample preparation, plasma proteins were precipitated with 0.1% acetic acid in acetonitrile. Waters UPLC BEH C18 (2.1mm I.D.×100mm, 1.7µm) column was used for chromatographic separation. A gradient elution of mobile phases consisting of 0.02% acetic acid in distilled water (solvent A) and 0.02% acetic acid in acetonitrile (solvent B) was used at a flow rate of 0.3mL/min. The multiple reaction monitoring (MRM) mode was used for mass spectrometric detection; the MRM transitions were m/z 1219.7→m/z 469.2 for hederacoside C and m/z 1108.3→m/z 221.2 for ginsenoside Rb1 (internal standard) in the negative ionization mode. A calibration curve was constructed in the range of 10-1000ng/mL. The intra- and inter-day precision and accuracy were within 5%. The developed UPLC-MS/MS method was successfully applied in a pharmacokinetic study of hederacoside C in rats. Hederacoside C was quickly but inadequately absorbed from the gastrointestinal tract of rats resulting in extremely low bioavailability and relatively slow clearance.
[Mh] Termos MeSH primário: Hedera
Ácido Oleanólico/análogos & derivados
Extratos Vegetais/sangue
Transtornos Respiratórios/sangue
Espectrometria de Massas em Tandem/métodos
[Mh] Termos MeSH secundário: Animais
Cromatografia Líquida de Alta Pressão/métodos
Masculino
Ácido Oleanólico/administração & dosagem
Ácido Oleanólico/sangue
Ácido Oleanólico/isolamento & purificação
Extratos Vegetais/administração & dosagem
Extratos Vegetais/isolamento & purificação
Ratos
Ratos Sprague-Dawley
Transtornos Respiratórios/tratamento farmacológico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Extracts); 0 (hederacoside C); 6SMK8R7TGJ (Oleanolic Acid)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170808
[Lr] Data última revisão:
170808
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160714
[St] Status:MEDLINE


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[PMID]:27217558
[Au] Autor:Huang Y; Wang Y; Tan L; Sun L; Petrosino J; Cui MZ; Hao F; Zhang M
[Ad] Endereço:Department of Biomedical Engineering, College of Engineering, The Ohio State University, Columbus, OH 43210; Interdisciplinary Biophysics Graduate Program, The Ohio State University, Columbus, OH 43210; Dorothy M. Davis Heart & Lung Research Institute, The Ohio State University Wexner Medical Ce
[Ti] Título:Nanospherical arabinogalactan proteins are a key component of the high-strength adhesive secreted by English ivy.
[So] Source:Proc Natl Acad Sci U S A;113(23):E3193-202, 2016 Jun 07.
[Is] ISSN:1091-6490
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Over 130 y have passed since Charles Darwin first discovered that the adventitious roots of English ivy (Hedera helix) exude a yellowish mucilage that promotes the capacity of this plant to climb vertical surfaces. Unfortunately, little progress has been made in elucidating the adhesion mechanisms underlying this high-strength adhesive. In the previous studies, spherical nanoparticles were observed in the viscous exudate. Here we show that these nanoparticles are predominantly composed of arabinogalactan proteins (AGPs), a superfamily of hydroxyproline-rich glycoproteins present in the extracellular spaces of plant cells. The spheroidal shape of the AGP-rich ivy nanoparticles results in a low viscosity of the ivy adhesive, and thus a favorable wetting behavior on the surface of substrates. Meanwhile, calcium-driven electrostatic interactions among carboxyl groups of the AGPs and the pectic acids give rise to the cross-linking of the exuded adhesive substances, favor subsequent curing (hardening) via formation of an adhesive film, and eventually promote the generation of mechanical interlocking between the adventitious roots of English ivy and the surface of substrates. Inspired by these molecular events, a reconstructed ivy-mimetic adhesive composite was developed by integrating purified AGP-rich ivy nanoparticles with pectic polysaccharides and calcium ions. Information gained from the subsequent tensile tests, in turn, substantiated the proposed adhesion mechanisms underlying the ivy-derived adhesive. Given that AGPs and pectic polysaccharides are also observed in bioadhesives exuded by other climbing plants, the adhesion mechanisms revealed by English ivy may forward the progress toward understanding the general principles underlying diverse botanic adhesives.
[Mh] Termos MeSH primário: Adesivos/química
Hedera/química
Mucoproteínas/química
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Sequência de Bases
Cálcio/química
Reagentes para Ligações Cruzadas
DNA de Plantas/genética
Hedera/genética
Microscopia de Força Atômica
Modelos Moleculares
Estrutura Molecular
Mucoproteínas/genética
Mucoproteínas/ultraestrutura
Nanosferas/química
Nanosferas/ultraestrutura
Pectinas/química
Proteínas de Plantas/química
Proteínas de Plantas/genética
Proteínas de Plantas/ultraestrutura
Molhabilidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Adhesives); 0 (Cross-Linking Reagents); 0 (DNA, Plant); 0 (Mucoproteins); 0 (Pectins); 0 (Plant Proteins); 0 (arabinogalactan proteins); 89NA02M4RX (pectin); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170802
[Lr] Data última revisão:
170802
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160525
[St] Status:MEDLINE
[do] DOI:10.1073/pnas.1600406113


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[PMID]:27183712
[Au] Autor:Schulte-Michels J; Runkel F; Gokorsch S; Häberlein H
[Ti] Título:Ivy leaves dry extract EA 575® decreases LPS-induced IL-6 release from murine macrophages.
[So] Source:Pharmazie;71(3):158-61, 2016 Mar.
[Is] ISSN:0031-7144
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:IL-6 plays a key role in the course of inflammatory processes as well as in the regulation of immune responses by the release of different cytokines. IL-6 is produced e.g. by macrophages recruited to the airways in response to a variety of inflammatory stimuli like allergens and respiratory viruses. Patients with inflammatory airway diseases therefore may benefit from therapies targeting the IL-6 pathway, e.g. reduction of the IL-6 release. Within this context, we tested the influence of the ivy leaves dry extract EA 575® on the LPS-induced release of IL-6 from murine macrophages (J774.2). One point seven µg/ml (5 µM) corticosterone served as positive control and was able to reduce LPS-induced IL-6 release by 46 ± 4%. EA 575® was tested in concentrations between 40 and 400 µg/ml. EA 575® decreased the LPS-induced IL-6 release in a dose-dependent manner and statistically significant by 25 ± 4%, 32 ± 4%, and 40 ± 7% in concentrations of 80, 160, and 400 µg/ml, respectively. The present data suggest an anti-inflammatory effect of EA 575® used in therapy of chronic- and acute inflammatory airway diseases accompanied with cough.
[Mh] Termos MeSH primário: Hedera/química
Interleucina-6/metabolismo
Lipopolissacarídeos/antagonistas & inibidores
Macrófagos/metabolismo
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Animais
Anti-Inflamatórios/farmacologia
Corticosterona/farmacologia
Relação Dose-Resposta a Droga
Macrófagos/efeitos dos fármacos
Camundongos
Extratos Vegetais/química
Folhas de Planta/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (EA 575 extract); 0 (Interleukin-6); 0 (Lipopolysaccharides); 0 (Plant Extracts); 0 (interleukin-6, mouse); W980KJ009P (Corticosterone)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:160517
[Lr] Data última revisão:
160517
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160518
[St] Status:MEDLINE


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[PMID]:26902407
[Au] Autor:Schulte-Michels J; Wolf A; Aatz S; Engelhard K; Sieben A; Martinez-Osuna M; Häberlein F; Häberlein H
[Ad] Endereço:Institute of Biochemistry and Molecular Biology, Rheinische Friedrich-Wilhelms-University of Bonn, Bonn, Germany.
[Ti] Título:α-Hederin inhibits G protein-coupled receptor kinase 2-mediated phosphorylation of ß2-adrenergic receptors.
[So] Source:Phytomedicine;23(1):52-7, 2016 Jan 15.
[Is] ISSN:1618-095X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Recently is has been shown that α- and ß-hederin increase the ß2-adrenergic responsiveness of alveolar type II cells (A549) and human airway smooth muscle cells (HASM), respectively, by inhibiting the internalization of ß2-adrenergic receptors (ß2AR) under stimulating conditions. Internalization of ß2AR is initiated by phosphorylations of certain serines and threonines by cAMP dependent protein kinase A (PKA) and G protein-coupled receptor kinases (GRK). PURPOSE: To evaluate the effect of α-hederin on PKA and GRK2 mediated phosphorylation of GFP-tagged ß2AR. STUDY DESIGN: To study this process we performed In-Cell Western using isoprenaline stimulated HEK293 cells overexpressing ß2AR as GFP fusion protein and specific antibodies against PKA (Ser345/346) and GRK2 (Ser355/356) phosphorylation sites. RESULTS: There was no effect found on the PKA mediated phosphorylation (n = 14) but we could show that α-hederin (1 µM, 12 h) significantly inhibits GRK2 mediated phosphorylation at Ser355/356 by 11 ± 5% (n ≥ 29, p ≤ 0.01) under stimulating conditions compared to the positive control. In Förster resonance energy transfer (FRET) experiments using the isolated kinases in solution α-hederin did not show any influence neither to GRK2 nor to PKA. CONCLUSION: Taken together, these results indicate that α-hederin acts as an indirect GRK2 inhibitor leading to a reduced homologous desensitization of ß2AR-GFP in HEK293 cells.
[Mh] Termos MeSH primário: Quinase 2 de Receptor Acoplado a Proteína G/metabolismo
Ácido Oleanólico/análogos & derivados
Receptores Adrenérgicos beta 2/metabolismo
Saponinas/farmacologia
[Mh] Termos MeSH secundário: Proteínas Quinases Dependentes de AMP Cíclico/metabolismo
Quinase 2 de Receptor Acoplado a Proteína G/antagonistas & inibidores
Células HEK293
Hedera/química
Seres Humanos
Isoproterenol/farmacologia
Ácido Oleanólico/farmacologia
Fosforilação
Proteínas Recombinantes de Fusão/metabolismo
Transdução de Sinais/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Receptors, Adrenergic, beta-2); 0 (Recombinant Fusion Proteins); 0 (Saponins); 27013-91-8 (alpha-hederin); 6SMK8R7TGJ (Oleanolic Acid); EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases); EC 2.7.11.15 (ADRBK1 protein, human); EC 2.7.11.16 (G-Protein-Coupled Receptor Kinase 2); L628TT009W (Isoproterenol)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:161126
[Lr] Data última revisão:
161126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160224
[St] Status:MEDLINE


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[PMID]:26837213
[Au] Autor:Klughammer C; Schreiber U
[Ad] Endereço:Julius-von-Sachs Institut für Biowissenschaften, Universität Würzburg, Julius-von-Sachs Platz 2, 97082, Würzburg, Germany.
[Ti] Título:Deconvolution of ferredoxin, plastocyanin, and P700 transmittance changes in intact leaves with a new type of kinetic LED array spectrophotometer.
[So] Source:Photosynth Res;128(2):195-214, 2016 May.
[Is] ISSN:1573-5079
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A newly developed compact measuring system for assessment of transmittance changes in the near-infrared spectral region is described; it allows deconvolution of redox changes due to ferredoxin (Fd), P700, and plastocyanin (PC) in intact leaves. In addition, it can also simultaneously measure chlorophyll fluorescence. The major opto-electronic components as well as the principles of data acquisition and signal deconvolution are outlined. Four original pulse-modulated dual-wavelength difference signals are measured (785-840 nm, 810-870 nm, 870-970 nm, and 795-970 nm). Deconvolution is based on specific spectral information presented graphically in the form of 'Differential Model Plots' (DMP) of Fd, P700, and PC that are derived empirically from selective changes of these three components under appropriately chosen physiological conditions. Whereas information on maximal changes of Fd is obtained upon illumination after dark-acclimation, maximal changes of P700 and PC can be readily induced by saturating light pulses in the presence of far-red light. Using the information of DMP and maximal changes, the new measuring system enables on-line deconvolution of Fd, P700, and PC. The performance of the new device is demonstrated by some examples of practical applications, including fast measurements of flash relaxation kinetics and of the Fd, P700, and PC changes paralleling the polyphasic fluorescence rise upon application of a 300-ms pulse of saturating light.
[Mh] Termos MeSH primário: Clorofila/metabolismo
Ferredoxinas/metabolismo
Hedera/metabolismo
Plastocianina/metabolismo
Espectrofotometria/instrumentação
[Mh] Termos MeSH secundário: Fluorescência
Hedera/efeitos da radiação
Cinética
Luz
Oxirredução
Folhas de Planta/metabolismo
Folhas de Planta/efeitos da radiação
Espectrofotometria/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ferredoxins); 1406-65-1 (Chlorophyll); 53321-11-2 (chlorophyll P 700); 9014-09-9 (Plastocyanin)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160204
[St] Status:MEDLINE
[do] DOI:10.1007/s11120-016-0219-0



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