| [PMID]: | 27217558 |
| [Au] Autor: | Huang Y; Wang Y; Tan L; Sun L; Petrosino J; Cui MZ; Hao F; Zhang M |
| [Ad] Endereço: | Department of Biomedical Engineering, College of Engineering, The Ohio State University, Columbus, OH 43210; Interdisciplinary Biophysics Graduate Program, The Ohio State University, Columbus, OH 43210; Dorothy M. Davis Heart & Lung Research Institute, The Ohio State University Wexner Medical Ce |
| [Ti] Título: | Nanospherical arabinogalactan proteins are a key component of the high-strength adhesive secreted by English ivy. |
| [So] Source: | Proc Natl Acad Sci U S A;113(23):E3193-202, 2016 Jun 07. |
| [Is] ISSN: | 1091-6490 |
| [Cp] País de publicação: | United States |
| [La] Idioma: | eng |
| [Ab] Resumo: | Over 130 y have passed since Charles Darwin first discovered that the adventitious roots of English ivy (Hedera helix) exude a yellowish mucilage that promotes the capacity of this plant to climb vertical surfaces. Unfortunately, little progress has been made in elucidating the adhesion mechanisms underlying this high-strength adhesive. In the previous studies, spherical nanoparticles were observed in the viscous exudate. Here we show that these nanoparticles are predominantly composed of arabinogalactan proteins (AGPs), a superfamily of hydroxyproline-rich glycoproteins present in the extracellular spaces of plant cells. The spheroidal shape of the AGP-rich ivy nanoparticles results in a low viscosity of the ivy adhesive, and thus a favorable wetting behavior on the surface of substrates. Meanwhile, calcium-driven electrostatic interactions among carboxyl groups of the AGPs and the pectic acids give rise to the cross-linking of the exuded adhesive substances, favor subsequent curing (hardening) via formation of an adhesive film, and eventually promote the generation of mechanical interlocking between the adventitious roots of English ivy and the surface of substrates. Inspired by these molecular events, a reconstructed ivy-mimetic adhesive composite was developed by integrating purified AGP-rich ivy nanoparticles with pectic polysaccharides and calcium ions. Information gained from the subsequent tensile tests, in turn, substantiated the proposed adhesion mechanisms underlying the ivy-derived adhesive. Given that AGPs and pectic polysaccharides are also observed in bioadhesives exuded by other climbing plants, the adhesion mechanisms revealed by English ivy may forward the progress toward understanding the general principles underlying diverse botanic adhesives. |
| [Mh] Termos MeSH primário: |
Adesivos/química
Hedera/química
Mucoproteínas/química
|
| [Mh] Termos MeSH secundário: |
Sequência de Aminoácidos
Sequência de Bases
Cálcio/química
Reagentes para Ligações Cruzadas
DNA de Plantas/genética
Hedera/genética
Microscopia de Força Atômica
Modelos Moleculares
Estrutura Molecular
Mucoproteínas/genética
Mucoproteínas/ultraestrutura
Nanosferas/química
Nanosferas/ultraestrutura
Pectinas/química
Proteínas de Plantas/química
Proteínas de Plantas/genética
Proteínas de Plantas/ultraestrutura
Molhabilidade
|
| [Pt] Tipo de publicação: | JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S. |
[Nm] Nome de substância:
| 0 (Adhesives); 0 (Cross-Linking Reagents); 0 (DNA, Plant); 0 (Mucoproteins); 0 (Pectins); 0 (Plant Proteins); 0 (arabinogalactan proteins); 89NA02M4RX (pectin); SY7Q814VUP (Calcium) |
| [Em] Mês de entrada: | 1701 |
| [Cu] Atualização por classe: | 170802 |
[Lr] Data última revisão:
| 170802 |
| [Sb] Subgrupo de revista: | IM |
| [Da] Data de entrada para processamento: | 160525 |
| [St] Status: | MEDLINE |
| [do] DOI: | 10.1073/pnas.1600406113 |
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